促胃肠动力药对活体SD大鼠下消化道电活动影响的研究
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摘要
目的:研究促胃肠动力药:多潘立酮、莫沙必利、伊托必利和克拉霉素对下消化道电活动的影响,及相互之间作用的比较;并进一步探讨其作用机制,从而为临床提供参考意见。
方法:采用SD大鼠活体埋置电极法,设多潘立酮组、莫沙比利组、伊托比利组、克拉霉素组以及生理盐水对照组,用生物机能实验系统BL-420E分别记录其对SD大鼠回肠及结肠快波振幅及频率的影响;然后加用阿托品、酚妥拉明及普萘洛尔作为拮抗剂,进一步探讨以上促胃肠动力药的作用机制。
结果:1.莫沙比利组、伊托比利组及克拉霉素组加用促动力药后均能够提高SD大鼠回肠及结肠快波的振幅和频率,与加药前比较有显著性差异(P＜0.05);而加用多潘立酮和等体积生理盐水后SD大鼠回肠及结肠快波振幅和频率的改变无统计学意义。
2.莫沙比利组、伊托比利组及克拉霉素组均能提高SD大鼠回肠及结肠快波振幅和频率的作用,与多潘立酮组及生理盐水对照组比较有显著性差异(P＜0.05),其中以伊托比利组的作用最明显(P＜0.05),克拉霉素组与莫沙比利组比较无显著性差异。
3.阿托品能够抑制莫沙比利组、伊托比利组、克拉霉素组及生理盐水对照组快波的振幅及频率(P＜0.05);而酚妥拉明及普萘洛尔对莫沙比利组、伊托比利组、克拉霉素组及生理盐水组的影响均无统计学意义。
结论:1.伊托必利、莫沙必利和克拉霉素均能够提高SD大鼠下消化道快波的振幅及频率,前者的作用优于后两者;而多潘立酮对其无明显影响。
2.伊托必利、莫沙必利和克拉霉素的其作用机制之一可能与胆碱能途径有关;而与肾上腺素能受体无关。
Objective: Contriving this experiment to investigate the effects of the domperidone, mosapride, itopride,and clarithromycin to the Sprague-Dawley's ileum and colon's fast wave,and further approach the mechanism of these drugs,thus providing accordings to clinic.
Methods: Imbed electrode in the body of the Sprague- Dawley rat,and set up the domperidone group, mosapride group, itopride, clarithromycin group and normal saline control group, using BL-420E to record effects of these drugs to the Sprague- Dawley's ileum and colon's fast wave; then add antagonists :atropine, phentolamine, and propranolol to further approach the mechanism of the motor drugs.
Results: 1. Mosapride, itopride,and clarithromycin can raise the amplitude and frequency of the Sprague- Dawley's ileum and colon comparing with before;but domperidone and normal saline can not produce obviously effect on the electric activity to Sprague- Dawley.
2. Mosapride, itopride,and clarithromycin can do obviously effect on the amplitude and frequency of the Sprague-Dawley's ileum and colon comparing with normal saline and domperidone,and the itopride is the most notable.There are no difference between mosapride and clarithromycin. Domperidon has no difference to the electric activity comparing with normal saline.
3.Atropine can depress the the amplitude and frequency of the Sprague- Dawley's ileum and colon in mosapride, itopride, clarithromycin, and normal saline group;but phentolamine and propranolol can not depress the action of the motor drugs.
Conclusion:1. Mosapride,itopride,clarithromycin can raise the amplitude and frequency of the Sprague- Dawley's lower digestive tract.The former is more obvious comparing with the latter.But domperidone has no effect.
2. Cholinergic pathway maybe is one of the mechanism of both of mosapride,itopride,clarithromycin; but there are no correlation to adnephrc pathway.
方法:采用SD大鼠活体埋置电极法,设多潘立酮组、莫沙比利组、伊托比利组、克拉霉素组以及生理盐水对照组,用生物机能实验系统BL-420E分别记录其对SD大鼠回肠及结肠快波振幅及频率的影响;然后加用阿托品、酚妥拉明及普萘洛尔作为拮抗剂,进一步探讨以上促胃肠动力药的作用机制。
结果:1.莫沙比利组、伊托比利组及克拉霉素组加用促动力药后均能够提高SD大鼠回肠及结肠快波的振幅和频率,与加药前比较有显著性差异(P＜0.05);而加用多潘立酮和等体积生理盐水后SD大鼠回肠及结肠快波振幅和频率的改变无统计学意义。
2.莫沙比利组、伊托比利组及克拉霉素组均能提高SD大鼠回肠及结肠快波振幅和频率的作用,与多潘立酮组及生理盐水对照组比较有显著性差异(P＜0.05),其中以伊托比利组的作用最明显(P＜0.05),克拉霉素组与莫沙比利组比较无显著性差异。
3.阿托品能够抑制莫沙比利组、伊托比利组、克拉霉素组及生理盐水对照组快波的振幅及频率(P＜0.05);而酚妥拉明及普萘洛尔对莫沙比利组、伊托比利组、克拉霉素组及生理盐水组的影响均无统计学意义。
结论:1.伊托必利、莫沙必利和克拉霉素均能够提高SD大鼠下消化道快波的振幅及频率,前者的作用优于后两者;而多潘立酮对其无明显影响。
2.伊托必利、莫沙必利和克拉霉素的其作用机制之一可能与胆碱能途径有关;而与肾上腺素能受体无关。
Objective: Contriving this experiment to investigate the effects of the domperidone, mosapride, itopride,and clarithromycin to the Sprague-Dawley's ileum and colon's fast wave,and further approach the mechanism of these drugs,thus providing accordings to clinic.
Methods: Imbed electrode in the body of the Sprague- Dawley rat,and set up the domperidone group, mosapride group, itopride, clarithromycin group and normal saline control group, using BL-420E to record effects of these drugs to the Sprague- Dawley's ileum and colon's fast wave; then add antagonists :atropine, phentolamine, and propranolol to further approach the mechanism of the motor drugs.
Results: 1. Mosapride, itopride,and clarithromycin can raise the amplitude and frequency of the Sprague- Dawley's ileum and colon comparing with before;but domperidone and normal saline can not produce obviously effect on the electric activity to Sprague- Dawley.
2. Mosapride, itopride,and clarithromycin can do obviously effect on the amplitude and frequency of the Sprague-Dawley's ileum and colon comparing with normal saline and domperidone,and the itopride is the most notable.There are no difference between mosapride and clarithromycin. Domperidon has no difference to the electric activity comparing with normal saline.
3.Atropine can depress the the amplitude and frequency of the Sprague- Dawley's ileum and colon in mosapride, itopride, clarithromycin, and normal saline group;but phentolamine and propranolol can not depress the action of the motor drugs.
Conclusion:1. Mosapride,itopride,clarithromycin can raise the amplitude and frequency of the Sprague- Dawley's lower digestive tract.The former is more obvious comparing with the latter.But domperidone has no effect.
2. Cholinergic pathway maybe is one of the mechanism of both of mosapride,itopride,clarithromycin; but there are no correlation to adnephrc pathway.
引文
[1]Hveem K,Svebak S,Hausken T,et al.Scand J Gastroenterol,1998;33(2):123
[2]姚希贤.临床消化病学.天津科技出版社,1995.618-624
[3]黄象谦.胃肠道疾病治疗学.天津科学技术出版社,1996:508
[4]李乾构,功能性消化不良的辨证论治探析.中国中西医结合脾胃杂志,19964(2):11
[5]姚泰.生理学.人民卫生出版社第六版.1978:183-190
[6]朴莲花 许文燮.胃肠道起搏细胞Cajal间质细胞的电生理学研究现状.延边大学学报 2005;3:227-230
[7]Sanders KM,Koh SD,Ward SM.Interstitial cells of cajal as pacemakers in the gastrointestinal tract Annu Rev Physiol 2006;68:307-343
[8]Sean MW,Tamas O,Julia RB,et al..Development of interstitial cells of Cajal and pacemaking in mice lacking enteric nerves[J].Gastroenterology 1999;117:584
[9]Ward SM.Sanders KM.Physiology and pathophysiology of the interstitial cell of Cajal;from bench to bedside.I.Functional development and plasticity of interstitial cells of Cajal networks.Am J Physiol Gastrointetest Liver Physiol 2001;281(3):602
[10]Hanani M,Freund HR.interstitial cells of Cajal-their role in pacing and signal transmission in the digestive system.Acta Phsiol Scand 2000;170(3):177
[11]Horiguchi K.Semple GS.Sanders KM.Ward SM.Distribution of pacemaker function through the tunica muscularis of the canine gastric antrum.J Physiol 2001;537(Pt 1):237
[12]TakayamaIHoriguchi K.Daigo Y.Mine T.Fujino MA.Ohno S.The interstitial cells of Cajal and a gastroenteric pacemaker system.Arch Histol Cytol 2002;65(1):1
[13]杨敏.房殿春.胃肠起搏治疗胃肠动力紊乱性疾病.中国医疗器械信息2003;9(4):14-17
[14]房殿春.胃肠起搏治疗胃肠动力紊乱性疾病.解放军医学杂志 2004;29(10):850-853
[15]张根葆.左保华.Cajal间质细胞与胃肠起搏.生理科学进展 2001;32(1):74-76
[16]徐爱忠,彭洪云.Cajal间质细胞研究进展.世界华人消化杂志.2006;14(24):2432-2435
[17]Ordog T Ward SM Sanders KM.Interstitial cells of Cajal generate electrical slow waves in the murine stomach.J Physiol 1999;518(Pt 1):257
[18]Tack J.Functional dyspepsia:impaired fundic accommodation.Curr Treat Options Gastroenterol,2000,3:287-294.
[19]Schelven IJ Nieuvenhuljs L Akkermans LM.Automated quantative analysis of interdigestive small intestinal myoelectric activity in rats.Neurogastroenterol Motil 2002;14(1):15
[20]Mellander A Mattsson A Svennerholm AM.Relationship between interdigestive motility and serection of immunoglobulin A in humanJ DigDisSci;1997,42(3):554-67
[21]JouetP Sarna S K Singaram C.Immunocytes and abnormal gas trointestinal motor activity duringileitis in dogsJ.Am J Physiol 1995;269(6pt 1):G913-24
[22]Fandriks L Mattsson A Dalenback J.Gastric output of IgA in man relation to migrating motility complexes and shum feedings J.Scand J Gastroenterol 1995;30(7):657-63
[23]郭宏 杨德治.胃肠移行性肌电复合波的发生机制.南京铁道医学院学报2000;19(2):141-144
[24]柯美云.胃肠动力学.科学出版社.1996:17-26
[25]Kuschinsky G,Lullmann H.Kurzes Lehrbuch der Pharmacologie,4 de druk.Stuttgart:Georg Thieme,1996
[26]Haug TT,Wilhmsen I,Ursin H,et al.What are the real Problems for Patients with functional dyspepsia?[J].Scand J Gastroenterol 1995;30(2):97
[27]杨敏,房殿春,龙庆林等.胃起搏加速胃动力紊乱犬的胃排空和纠正异常胃电节律.中华消化杂志;2003;23(5):137
[2]姚希贤.临床消化病学.天津科技出版社,1995.618-624
[3]黄象谦.胃肠道疾病治疗学.天津科学技术出版社,1996:508
[4]李乾构,功能性消化不良的辨证论治探析.中国中西医结合脾胃杂志,19964(2):11
[5]姚泰.生理学.人民卫生出版社第六版.1978:183-190
[6]朴莲花 许文燮.胃肠道起搏细胞Cajal间质细胞的电生理学研究现状.延边大学学报 2005;3:227-230
[7]Sanders KM,Koh SD,Ward SM.Interstitial cells of cajal as pacemakers in the gastrointestinal tract Annu Rev Physiol 2006;68:307-343
[8]Sean MW,Tamas O,Julia RB,et al..Development of interstitial cells of Cajal and pacemaking in mice lacking enteric nerves[J].Gastroenterology 1999;117:584
[9]Ward SM.Sanders KM.Physiology and pathophysiology of the interstitial cell of Cajal;from bench to bedside.I.Functional development and plasticity of interstitial cells of Cajal networks.Am J Physiol Gastrointetest Liver Physiol 2001;281(3):602
[10]Hanani M,Freund HR.interstitial cells of Cajal-their role in pacing and signal transmission in the digestive system.Acta Phsiol Scand 2000;170(3):177
[11]Horiguchi K.Semple GS.Sanders KM.Ward SM.Distribution of pacemaker function through the tunica muscularis of the canine gastric antrum.J Physiol 2001;537(Pt 1):237
[12]TakayamaIHoriguchi K.Daigo Y.Mine T.Fujino MA.Ohno S.The interstitial cells of Cajal and a gastroenteric pacemaker system.Arch Histol Cytol 2002;65(1):1
[13]杨敏.房殿春.胃肠起搏治疗胃肠动力紊乱性疾病.中国医疗器械信息2003;9(4):14-17
[14]房殿春.胃肠起搏治疗胃肠动力紊乱性疾病.解放军医学杂志 2004;29(10):850-853
[15]张根葆.左保华.Cajal间质细胞与胃肠起搏.生理科学进展 2001;32(1):74-76
[16]徐爱忠,彭洪云.Cajal间质细胞研究进展.世界华人消化杂志.2006;14(24):2432-2435
[17]Ordog T Ward SM Sanders KM.Interstitial cells of Cajal generate electrical slow waves in the murine stomach.J Physiol 1999;518(Pt 1):257
[18]Tack J.Functional dyspepsia:impaired fundic accommodation.Curr Treat Options Gastroenterol,2000,3:287-294.
[19]Schelven IJ Nieuvenhuljs L Akkermans LM.Automated quantative analysis of interdigestive small intestinal myoelectric activity in rats.Neurogastroenterol Motil 2002;14(1):15
[20]Mellander A Mattsson A Svennerholm AM.Relationship between interdigestive motility and serection of immunoglobulin A in humanJ DigDisSci;1997,42(3):554-67
[21]JouetP Sarna S K Singaram C.Immunocytes and abnormal gas trointestinal motor activity duringileitis in dogsJ.Am J Physiol 1995;269(6pt 1):G913-24
[22]Fandriks L Mattsson A Dalenback J.Gastric output of IgA in man relation to migrating motility complexes and shum feedings J.Scand J Gastroenterol 1995;30(7):657-63
[23]郭宏 杨德治.胃肠移行性肌电复合波的发生机制.南京铁道医学院学报2000;19(2):141-144
[24]柯美云.胃肠动力学.科学出版社.1996:17-26
[25]Kuschinsky G,Lullmann H.Kurzes Lehrbuch der Pharmacologie,4 de druk.Stuttgart:Georg Thieme,1996
[26]Haug TT,Wilhmsen I,Ursin H,et al.What are the real Problems for Patients with functional dyspepsia?[J].Scand J Gastroenterol 1995;30(2):97
[27]杨敏,房殿春,龙庆林等.胃起搏加速胃动力紊乱犬的胃排空和纠正异常胃电节律.中华消化杂志;2003;23(5):137