血浆网膜素-1在2型糖尿病合并冠心病中的变化及意义
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摘要
目的
2型糖尿病以心血管疾病的患病及死亡风险增加为主要特征,相对于非2型糖尿病而言,2型糖尿病患者心血管患病风险约为其2-3倍,但对连接2型糖尿病与其心血管并发症的潜在作用机制所知有限。研究表明其病理改变是动脉粥样硬化,主要病理基础是由多因素相互协作所形成的慢性、亚临床炎症以及炎症导致的胰岛素抵抗。血浆网膜素-1(Omentin-1)是新近发现的一个脂肪细胞因子,研究发现其与胰岛素抵抗、糖尿病、动脉粥样硬化、肥胖等关系密切。本研究通过检测不同组别患者的血浆网膜素-1(Omentin-1)水平,分析血浆Omentin-1水平与BMI、血压、糖脂代谢指标、hsCRP、IL-6水平间的关系,探讨血浆Omentin-1与2型糖尿病并发冠心病的关系,为临床合理防治2型糖尿病及其心血管并发症提供新的思路。
研究对象和方法
选取2010年5月至2011年8月郑州大学第一附属医院老年内分泌科住院的70例2型糖尿病患者,按照是否伴有冠心病分为单纯2型糖尿病组36例(T2DM组)、2型糖尿病合并冠心病组34例(T2DM+CHD组)。另选取性别及年龄相匹配的29例单纯冠心病患者(CHD组)及39例健康体检者为健康对照组(HC组)。四组均统计性别、年龄、收缩压(SBP)、舒张压(DBP)、BMI、空腹血糖(FBG)、糖化血红蛋白(HbAlc)、血清空腹胰岛素(Fins)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、高敏C反应蛋白(hsCRP)水平,计算LDL-C/HDL-C,并用稳态模型计算胰岛素抵抗指数HOMA-IR。同时采用酶联免疫吸附法(ELISA)测定血浆Omentin-1、IL-6水平。应用SPSS17.0软件进行数据处理,检验数据的正态性及方差齐性,组间比较采用单因素方差分析。Omentin-1与其他指标的相关性应用Pearson相关分析、stepwise逐步回归分析,以P<0.05为具有统计学意义。
结果
1.CHD组、T2DM组及T2DM+CHD组患者血浆Omentin-1水平明显低于HC组(P<0.05)。T2DM+CHD组患者血浆Omentin-1水平明显低于CHD组(392.97±9.97vs2017.33±8.86,P<0.01)。
2. Omentin-1与BMI、SBP、DBP、HbAlc、HOMA-IR、TG、LDL-C/HDL-C、 IL-6呈负相关(P<0.05)。
3. BMI、HOMA-IR、IL-6是影响血浆Omentin-1的独立危险因素(P=0.043、P=0.016、P=0.003)。
结论
1.血浆Omentin-1的降低可能参与了2型糖尿病、冠心病特别是2型糖尿病并发冠状动脉粥样硬化的发生发展过程。
2.血浆Omentin-1可能通过改善胰岛素抵抗、发挥抗炎作用、保护血管内皮功能、调节糖脂代谢等机制参与了2型糖尿病及其冠状动脉粥样硬化的发生及发展过程。
3. BMI、HOMA-IR、IL-6成为影响血浆Omentin-1水平最为显著的因素。提高血浆Omentin-1水平可能有助于预防2型糖尿病及其心血管并发症的发生。
Background and objective
Diabetes has reached pandemic proportions and is associated with serious cardio-metabolic sequelae. It is well known that T2DM is closely linked to obesity, insulin resistance, and inflammation. Subjects with T2DM also have increased cardiovascular morbidity and mortality. A historical cohort study spanning over a22-year period reported that compared with non-diabetic controls, patients with T2DM showed higher cardiovascular morbidity and higher cardiovascular mortality. Adipokines contribute directly to the atherosclerotic process, connecting metabolic disorders such as obesity and diabetes to cardiovascular disease. Omentin-1is a novel adipokine, the data about the relationship of this adipikine to vascularhealth in type2diabetes is limited. Our study is to investigate the level of plasma omentin-1and other clinical data, and to analyze the distinction between type2diabetes mellitus with coronary heart disease, type2diabetes mellitus, coronary heart disease and normal controls.
Research subjects and methods
A total of seventy patients with type2diabetes were divided into two groups: thirty-four patients with coronary heart disease (T2DM+CHD) and thirty-six patients without coronary heart disease (T2DM). They are from the inpatients of Geriatrics of the First Affiliated Hospital of Zhengzhou University from May2010to August2011. They are in line with1999WHO classification and diagnosis standards of diabetes. Another thirty-nine healthy control subjects (HC) and twenty-nine patients with coronary heart disease (CHD) were enrolled in this study. Plasma level of omentin-1were measured by enzyme-linked immunosorbent assay (ELISA), and other clinical markers were also measured. We analyzed the change tendency and influential factors of omentin-1in those control subjects. All the statistical analysis were dealt with SPSS17.0software.
Results
1. Plasma omentin-1level were found significantly decreased in patients with type2diabetes (1164.33±9.67ng/L)、patients with coronary heart disease(2017.33±8.86ng/L) and patients with type2diabetes and coronary heart disease (392.97±9.97ng/L) compared with healthy control (5764.23±9.62ng/L) at P<0.05or P<0.01.
2. Plasma omentin-1level correlated negatively with BMI、SBP、DBP、HbA1c、 HOMA-IR、TG、LDL-C/HDL-C and IL-6(P<0.05).
3. Multiple regression analysis showed that BMI、HOMA-IR and IL-6were the independent related factors influencing omentin-1level(P=0.043、P=0.016、P=0.003).
Conclusion
1. Plasma omentin-1level obviously decreased in patients with type2diabetes、 patients with coronary heart disease and patients with type2diabetes with coronary heart disease compared with healthy control. The findings suggest that omentin-1may associated with type2diabetes, coronary heart disease and type2diabetes complicated coronary atherosclerosis.
2. Plasma omentin-1level correlated negatively with BMI、SBPI、DBP、HbA1c、 HOMA-IR、TG、LDL-C/HDL-C、IL-6, indicating that omentin-1may play predominant roles as anti-inflammatory molecule, regulator of insulin-resistant and protector of vascular endothelial cell. Omentin-1may link to glucose and lipid metabolism and blood pressure, and it may contribute to the pathogenesis of IR and type2diabetes complicated coronary heart disease.
3. BMI、HOMA-IR、IL-6were the independent related factors in influencing the level of omentin-1. Thus, omentin-1plausibly may interact to glucose and lipid metabolism and inflammation. Thus, omentin-1may have beneficial effects on the type2diabetes and its complicated coronary heart disease, and could potentially be used as a biologic marker to those diseases.
2型糖尿病以心血管疾病的患病及死亡风险增加为主要特征,相对于非2型糖尿病而言,2型糖尿病患者心血管患病风险约为其2-3倍,但对连接2型糖尿病与其心血管并发症的潜在作用机制所知有限。研究表明其病理改变是动脉粥样硬化,主要病理基础是由多因素相互协作所形成的慢性、亚临床炎症以及炎症导致的胰岛素抵抗。血浆网膜素-1(Omentin-1)是新近发现的一个脂肪细胞因子,研究发现其与胰岛素抵抗、糖尿病、动脉粥样硬化、肥胖等关系密切。本研究通过检测不同组别患者的血浆网膜素-1(Omentin-1)水平,分析血浆Omentin-1水平与BMI、血压、糖脂代谢指标、hsCRP、IL-6水平间的关系,探讨血浆Omentin-1与2型糖尿病并发冠心病的关系,为临床合理防治2型糖尿病及其心血管并发症提供新的思路。
研究对象和方法
选取2010年5月至2011年8月郑州大学第一附属医院老年内分泌科住院的70例2型糖尿病患者,按照是否伴有冠心病分为单纯2型糖尿病组36例(T2DM组)、2型糖尿病合并冠心病组34例(T2DM+CHD组)。另选取性别及年龄相匹配的29例单纯冠心病患者(CHD组)及39例健康体检者为健康对照组(HC组)。四组均统计性别、年龄、收缩压(SBP)、舒张压(DBP)、BMI、空腹血糖(FBG)、糖化血红蛋白(HbAlc)、血清空腹胰岛素(Fins)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、高敏C反应蛋白(hsCRP)水平,计算LDL-C/HDL-C,并用稳态模型计算胰岛素抵抗指数HOMA-IR。同时采用酶联免疫吸附法(ELISA)测定血浆Omentin-1、IL-6水平。应用SPSS17.0软件进行数据处理,检验数据的正态性及方差齐性,组间比较采用单因素方差分析。Omentin-1与其他指标的相关性应用Pearson相关分析、stepwise逐步回归分析,以P<0.05为具有统计学意义。
结果
1.CHD组、T2DM组及T2DM+CHD组患者血浆Omentin-1水平明显低于HC组(P<0.05)。T2DM+CHD组患者血浆Omentin-1水平明显低于CHD组(392.97±9.97vs2017.33±8.86,P<0.01)。
2. Omentin-1与BMI、SBP、DBP、HbAlc、HOMA-IR、TG、LDL-C/HDL-C、 IL-6呈负相关(P<0.05)。
3. BMI、HOMA-IR、IL-6是影响血浆Omentin-1的独立危险因素(P=0.043、P=0.016、P=0.003)。
结论
1.血浆Omentin-1的降低可能参与了2型糖尿病、冠心病特别是2型糖尿病并发冠状动脉粥样硬化的发生发展过程。
2.血浆Omentin-1可能通过改善胰岛素抵抗、发挥抗炎作用、保护血管内皮功能、调节糖脂代谢等机制参与了2型糖尿病及其冠状动脉粥样硬化的发生及发展过程。
3. BMI、HOMA-IR、IL-6成为影响血浆Omentin-1水平最为显著的因素。提高血浆Omentin-1水平可能有助于预防2型糖尿病及其心血管并发症的发生。
Background and objective
Diabetes has reached pandemic proportions and is associated with serious cardio-metabolic sequelae. It is well known that T2DM is closely linked to obesity, insulin resistance, and inflammation. Subjects with T2DM also have increased cardiovascular morbidity and mortality. A historical cohort study spanning over a22-year period reported that compared with non-diabetic controls, patients with T2DM showed higher cardiovascular morbidity and higher cardiovascular mortality. Adipokines contribute directly to the atherosclerotic process, connecting metabolic disorders such as obesity and diabetes to cardiovascular disease. Omentin-1is a novel adipokine, the data about the relationship of this adipikine to vascularhealth in type2diabetes is limited. Our study is to investigate the level of plasma omentin-1and other clinical data, and to analyze the distinction between type2diabetes mellitus with coronary heart disease, type2diabetes mellitus, coronary heart disease and normal controls.
Research subjects and methods
A total of seventy patients with type2diabetes were divided into two groups: thirty-four patients with coronary heart disease (T2DM+CHD) and thirty-six patients without coronary heart disease (T2DM). They are from the inpatients of Geriatrics of the First Affiliated Hospital of Zhengzhou University from May2010to August2011. They are in line with1999WHO classification and diagnosis standards of diabetes. Another thirty-nine healthy control subjects (HC) and twenty-nine patients with coronary heart disease (CHD) were enrolled in this study. Plasma level of omentin-1were measured by enzyme-linked immunosorbent assay (ELISA), and other clinical markers were also measured. We analyzed the change tendency and influential factors of omentin-1in those control subjects. All the statistical analysis were dealt with SPSS17.0software.
Results
1. Plasma omentin-1level were found significantly decreased in patients with type2diabetes (1164.33±9.67ng/L)、patients with coronary heart disease(2017.33±8.86ng/L) and patients with type2diabetes and coronary heart disease (392.97±9.97ng/L) compared with healthy control (5764.23±9.62ng/L) at P<0.05or P<0.01.
2. Plasma omentin-1level correlated negatively with BMI、SBP、DBP、HbA1c、 HOMA-IR、TG、LDL-C/HDL-C and IL-6(P<0.05).
3. Multiple regression analysis showed that BMI、HOMA-IR and IL-6were the independent related factors influencing omentin-1level(P=0.043、P=0.016、P=0.003).
Conclusion
1. Plasma omentin-1level obviously decreased in patients with type2diabetes、 patients with coronary heart disease and patients with type2diabetes with coronary heart disease compared with healthy control. The findings suggest that omentin-1may associated with type2diabetes, coronary heart disease and type2diabetes complicated coronary atherosclerosis.
2. Plasma omentin-1level correlated negatively with BMI、SBPI、DBP、HbA1c、 HOMA-IR、TG、LDL-C/HDL-C、IL-6, indicating that omentin-1may play predominant roles as anti-inflammatory molecule, regulator of insulin-resistant and protector of vascular endothelial cell. Omentin-1may link to glucose and lipid metabolism and blood pressure, and it may contribute to the pathogenesis of IR and type2diabetes complicated coronary heart disease.
3. BMI、HOMA-IR、IL-6were the independent related factors in influencing the level of omentin-1. Thus, omentin-1plausibly may interact to glucose and lipid metabolism and inflammation. Thus, omentin-1may have beneficial effects on the type2diabetes and its complicated coronary heart disease, and could potentially be used as a biologic marker to those diseases.
引文
[1]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2010年版)[S].中华糖尿病杂志,2010,(2)：6-8.
[2]Yang R Z,Lee M J,Hu H, et al. Identification of omentin as a noveldepot-specific adipokine in human adipose tissue:possible role in modulating insulin action[J]. Am J Physiol Endocrinol Metab,2006,290(6):E1253-1261.
[3]De Souza Batista C M,Yang R Z, Lee M J,et al. Omentin plasma levels and gene expression are decreased in obesity[J]. Diabetes,2007,56(6):1655-1661.
[4]Yu YH, Ginsberg HN. Adipocyte signaling and lipid homeostasis:sequelae of insulin-resistant adipose tissue[J]. Circ Res,2005,96(10):1042-1052.
[5]Wang Z, Nakayama T. Inflammation, a link between obesity and cardiovascular disease[J]. Mediators Inflamm,2010, Article ID:535918, Published online 2010 August 5. doi:10.1155/2010/535918.
[6]赵迪.内脂素及肿瘤坏死因子-α与2型糖尿病大血管并发症的关系[D].[硕士学位论文].河南：郑州大学：老年医学,2010.
[7]Moreno-Navarrete JM, Ortega F, Castro A, et al. Circulating omentin as a novel biomarker of endothelial dysfunction[J]. Obesity (Silver Spring),2011,19(8):1552-1559.
[8]Liu R, Wang X, Bu P. Omentin-1 is associated with carotid atherosclerosis in patients with metabolic syndrome[J]. Diabetes Res Clin Pract,2011,93(1):21-25.
[9]Shibata R, Takahashi R, Kataoka Y, et al. Association of a fat-derived plasma protein omentin with carotid artery intima-media thickness in apparently healthy men[J]. Hypertens Res,2011,34(12):1309-1312.
[10]El-Mesallamy HO, El-Derany MO, Hamdy NM. Serum omentin-1 and chemerin levels are interrelated in patients with Type 2 diabetes mellitus with or without ischaemic heart disease[J]. Diabet Med,2011,28(10):1194-1200.
[11]Zhong X, Zhang HY, Tan H, et al. Association of serum omentin-1 levels with coronary artery disease[J]. Acta Pharmacol Sin,2011,32(7):873-878.
[12]Hye Jin Yoo, Soon Young Hwang, Ho Cheol Hong, et al. Association of circulating omentin-1 level with arterial stiffness and carotid plaque in type 2 diabetes[J].Cardiovasc Diabetol,2011,10:103. Published online 2011 November 22. doi:10.1186/1475-2840-10-103.
[13]Pan H Y, Guo L, Li Q. Changes of serum omentin-1 levels in normal subjects and in patients with impaired glucose regulation and with newly diagnosed and untreated type 2 diabetes[J]. Diabetes Res Clin Pract,2010,88(1):29-33.
[14]Schaffler A, Zeitoun M, Wobser H. Frequency and significance of the novel single nucleotide missense polymorphism Val109Asp in the human gene encoding omentin in Caucasian patients with type 2 diabetes mellitus or chronic inflammatory bowel diseases[J]. Cardiovascular Diabetology,2007,6:3.DOI:10.1186/1475-2840-6-3.
[15]R.C. Cai, L. Wei, J.Z. Di, et al. Expression of omentin in adipose tissues in obese and type 2 diabetic patients[J]. Zhonghua Yi Xue Za Zhi,2009,89:381-384.
[16]李晓红.2型糖尿病并急性心肌梗死的临床分析[J].中原医刊,2008,35(8)：56-57.
[17]Yamawaki H,Tsubaki N,Mukohda M,et al.Omentin,a novel adipokine induces vasodilation in rat isolated blood cessels[J]. Biochem Biophys Res Commun,2010,393(4):668-672.
[18]毕艳,朱大龙,等.网膜素的研究进展[J].中国糖尿病杂志,2007,15(9),574-575.
[19]陈茜,牛建民,等,脂肪细胞因子网膜素-1与代谢综合征[J].实用医学杂志,2011,27(10)：1887-1889.
[20]Maruyama S, Shibata R, Kikuchi R, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism[J]. J Biol Chem,2012,287(1):408-417.
[21]Shang FJ, Wang JP, Liu XT, et al. Serum omentin-1 levels are inversely associated with the presence and severity of coronary artery disease in patients with metabolic syndrome[J]. Biomarkers,2011,16(8):657-662.
[22]Shibata R, Ouchi N, Kikuchi R, et al. Circulating omentin is associated with coronary artery disease in men[J]. Atherosclerosis,2011,219(2):811-814.
[23]Yamawaki H, Kuramoto J, Kameshima S, et al. Omentin, a novel adipocytokine inhibits TNF-induced vascular inflammation in human endothelial cells[J]. Biochem Biophys Res Commun,2011,408(2):339-343.
[24]Xie H, Xie PL, Wu XP, et al. Omentin-1 attenuates arterial calcification and bone loss in osteoprotegerin-deficient mice by inhibition of RANKL expression [J]. Cardiovasc Res, 2011,92(2):296-306.
[25]Maruyama S, Shibata R, Kikuchi R, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism[J]. J Biol Chem,2012,287(1):408-417.
[26]Yamawaki H.Vascular effects of novel adipocytokines:focus on vascular contractility and inflammatory responses [J]. Biol Pharm Bull,2011,34(3):307-310.
[27]B.K. Tan, R. Adya, S. Farhatullah, et al. Omentin-1, a novel adipokine, is decreased in overweight insulin resistant women with the polycystic ovary syndrome:ex vivo and in vivo regulation of omentin-1 by insulin and glucose[J]. Diabetes,2008,57:801-808.
[1]Rabe K, Lehrke M, Parhofer KG, et al. Adipokines and insulin resistance[J].Mol Med, 2008,14(11-12):741-751.
[2]Yang RZ, Xu A, Pray J, et al. Cloning of omentin, a new adipocytokine from omental fat tissue in human[J]. Diabetes,2005,52(suppll):A1.
[3]B.K. Tan, R. Adya, S. Farhatullah, et al. Omentin-1, a novel adipokine, is decreased in overweight insulin resistant women with the polycystic ovary syndrome:ex vivo and in vivo regulation of omentin-1 by insulin and glucose[J]. Diabetes,2008,57:801-808.
[4]De Souza Batista CM, Yang RZ, Lee MJ, et,al. Omentin plasma lecels and gene expression are decreased in obesity[J]. Diabetes,2007,56(6):1655-1661.
[5]Yang RZ, Lee MJ, Hu H, et al. Identification of omentin as a novel depot-specific adipokine in human adipose tissue:possible role in modulating insulin action[J]. Am J Physiol Endocrinol Metab,2006,290:E1253-1261.
[6]毕艳,朱大龙,龚大为.网膜素的研究进展[J].中国糖尿病杂志,2007,15(9)：574-575.
[7]Schaffler A, Neumeier M, Herfarth H, et al. Genomic structure of human omentin, a new adipocytokine expressed in omental adipose tissue[J]. Bilchim Biophys Acta,2005,1732: 96-102.
[8]Fain J N, Sacks H S,Buehrer B, et al. Identification of omentin mRNAin human epicaedial adipose tissue comparison to omentin in subcutaneous, internal mammary artery periadventitial and cisceral abdominal depots[J].Int J Obes,2008,32(5):810-815.
[9]Tsuji S, Uehori J, Matsumoto M, et al. Human intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell wall[J]. J Biol Chem, 2001,276:23456-23463.
[10]Pan H Y,Guo L, Li Q. Changes of serum omentin-1 levels in normal subjects and in patients with impaired glucose regulation and with newly diagnosed and untreated type 2 diabetes[J]. Diabetes Res Clin Pract,2010,88(1):9-33.
[11]Yoon J C, Chickering T W, Rosen E D, et al. Peroxisome proliferator-activated receptor gamma target gene encoding a novel angiopoietin-related protein associated with adipose differentiation[J]. Mol Cell Biol,2000,20:5343-5349.
[12]陈茜,牛建民,温济英,等.脂肪细胞因子网膜素-1与代谢综合征[J].实用医学杂志,2011,27(10)：1887-1889.
[13]Lee JK, Sxhnee J,Pang M, et al. Human homologs of the Xenopus oocyte cortical granule lectin XL35[J]. Glycobilogy,2001,11:65-73.
[14]Suzuki YA, Shin K, Lonnerdal B. Molecular cloning and fuctional expression of a human intestinal lactoferrin receptor[J]. Biochemistry,2001,40:15771-15779.
[15]J.M. Moreno-Navarrete, V. Catalan, F. Ortega, et al.Circulating omentin concentration increases after weight loss[J]. Nutr Metab (Lond),2010,7:27
[16]T.Komiya, Y. Tanigawa, S. Hirohashi. Cloning of the novel gene intelectin, which is expressed in intestinal Paneth cells in mice[J]. Biochem Biophys Res Commun,1998,251: 759-762.
[17]Cantarini L, Simonini G, Fioravanti A, et al. Circulating levels of the adipokines vaspin and omentin in patients with juvenile idiopathic arthritis, and relation to disease activity[J]. Clin Exp Rheumatol,2011,29(6):1044-1048.
[18]Tan BK, Adya R, Syed F,et al, Metformin trement may increase omentin-1 levels in women with polycystic ovary syndrome[J]. Diabetes,2010,59:3023-2031.
[19]Bee K. Tan. Raghu Adya, HArpal S. Randeva. Omentin:A novel link between inflammation, diabesity, and cardiovascular disease[J]. Trends Cardiocasc Med,2010, 20(5):143-148.
[20]Gustafson B. Adipose tissue,inflammation and atherosclerosis [J]. J Atheroscler Thromb, 2010,17(4):332-341.
[21]Orr A.W., Hastings N.E., Blackman B.R., et al. Complex regulation and function of the inflammatory smooth muscle cell phenotypa in atherosclerosis [J]. J Vasc Res,2010,47(2): 168-180.
[22]Yamawaki H, Tsubaki N, Mukohda M, et al. Omentin,a novel adipokine,induces vasodilation in rat isolated blood[J]. Biochem Biophys Res Commun,2010,393(4):668-672.
[23]Yamawaki H.Vascular effects of novel adipocytokines:focus on vascular contractility and inflammatory responses[J]. Biol Pharm Bull,2011,34(3):307-310.
[24]Xie H, Xie PL, Wu XP, et al. Omentin-1 attenuates arterial calcification and bone loss in osteoprotegerin-deficient mice by inhibition of RANKL expression[J]. Cardiovasc Res, 2011,92(2):296-306.
[25]Xie H, Xie PL, Luo XH, et al. Omentin-1 exerts bone-sparing effect in ovariectomized mice[J]. Osteoporos Int,2012,23(4):1425-1436.
[26]El-Mesallamy HO, El-Derany MO, Hamdy NM. Serum omentin-1 and chemerin levels are interrelated in patients with Type 2 diabetes mellitus with or without ischaemic heart disease[J]. Diabet Med,2011,28(10):1194-1200.
[27]R.C. Cai, L. Wei, J.Z. Di, et al. Expression of omentin in adipose tissues in obese and type 2 diabetic patients[J]. Zhonghua Yi Xue Za Zhi,2009,89:381-384.
[28]项守奎,冯文焕,毕艳,等.超重及肥胖人群血清网膜素-1水平的变化[J].中国糖尿病杂志,2009(1)：39-42.
[29]Wurm S, Neumeier M, Weigert J, et al. Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults[J]. Cardiovasc Diabetol,2007,20:6-7.
[30]Akbarzadeh S, Nabipour I, Assadi M, et al. The normoglycemic first-degree relatives of patients with type 2 diabetes mellitus have low circulating omentin-1 and adiponectin levels[J]. Cytokine,2012,58(2):295-299.
[31]Choi JH, Rhee EJ, Kim KH, et al. Plasma omentin-1 levels are reduced in non-obese women with normal glucose tolerance and polycystic ovary syndrome[J]. Eur J Endocrinol. 2011,165(5):789-796.
[32]Yan P, Li L, Yang M, et al. Effects of the long-acting human glucagon-like peptide-1 analog liraglutide on plasma omentin-1 levels in patients with type 2 diabetes mellitus[J]. Diabetes Res Clin Pract,2011,92(3):368-374.
[33]A. Saremi, M. Asghari, A. Ghorbani. Effects of aerobic training on serum omentin-1 and cardiometabolic risk factors in overweight and obese men[J]. J Sports Sci,2010,4:1-6
[34]Sacks HS, Fain JN. Human epicaedial adipose tissue:a reciew[J]. Am Heart J,2007,153: 907-917.
[35]M. Montagnani, L.V. Ravichandran, H. Chen, et al. Insulin receptor substrate-1 and phosphoinositide-dependent kinase-1 are required for insulin-stimulated production of nitric oxide in endothelial cells[J]. Mol Endocrinol,2002,16:1931-1942
[36]Maruyama S, Shibata R, Kikuchi R, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism[J]. J Biol Chem,2012,287(1):408-417.
[37]Shang FJ, Wang JP, Liu XT, et al. Serum omentin-1 levels are inversely associated with the presence and severity of coronary artery disease in patients with metabolic syndrome[J]. Biomarkers,2011,16(8):657-662.
[38]Shibata R, Takahashi R, Kataoka Y, et al. Association of a fat-derived plasma protein omentin with carotid artery intima-media thickness in apparently healthy men[J]. Hypertens Res,2011,34(12):1309-1312.
[39]Shibata R, Ouchi N, Kikuchi R, et al. Circulating omentin is associated with coronary artery disease in men[J]. Atherosclerosis,2011,219(2):811-814.
[40]许曼音,陆广华,陈名道.糖尿病学[M].上海：上海科学技术出版社,2010.471-479.
[41]赵迪,李华,杨再刚,等.内脂素及肿瘤坏死因子-α与2型糖尿病大血管并发症的关系[J].山东医药,2010,50(17)：48-50.
[42]Fu M, Gong DW, Damcott C, et al. Systematic analysis of omentin 1 and omentin 2 on 1q23 as candidate genes for type 2 diabetes in the Old Order Amish (Abstract) [J]. Diabetes, 2004,53:A59.
[2]Yang R Z,Lee M J,Hu H, et al. Identification of omentin as a noveldepot-specific adipokine in human adipose tissue:possible role in modulating insulin action[J]. Am J Physiol Endocrinol Metab,2006,290(6):E1253-1261.
[3]De Souza Batista C M,Yang R Z, Lee M J,et al. Omentin plasma levels and gene expression are decreased in obesity[J]. Diabetes,2007,56(6):1655-1661.
[4]Yu YH, Ginsberg HN. Adipocyte signaling and lipid homeostasis:sequelae of insulin-resistant adipose tissue[J]. Circ Res,2005,96(10):1042-1052.
[5]Wang Z, Nakayama T. Inflammation, a link between obesity and cardiovascular disease[J]. Mediators Inflamm,2010, Article ID:535918, Published online 2010 August 5. doi:10.1155/2010/535918.
[6]赵迪.内脂素及肿瘤坏死因子-α与2型糖尿病大血管并发症的关系[D].[硕士学位论文].河南：郑州大学：老年医学,2010.
[7]Moreno-Navarrete JM, Ortega F, Castro A, et al. Circulating omentin as a novel biomarker of endothelial dysfunction[J]. Obesity (Silver Spring),2011,19(8):1552-1559.
[8]Liu R, Wang X, Bu P. Omentin-1 is associated with carotid atherosclerosis in patients with metabolic syndrome[J]. Diabetes Res Clin Pract,2011,93(1):21-25.
[9]Shibata R, Takahashi R, Kataoka Y, et al. Association of a fat-derived plasma protein omentin with carotid artery intima-media thickness in apparently healthy men[J]. Hypertens Res,2011,34(12):1309-1312.
[10]El-Mesallamy HO, El-Derany MO, Hamdy NM. Serum omentin-1 and chemerin levels are interrelated in patients with Type 2 diabetes mellitus with or without ischaemic heart disease[J]. Diabet Med,2011,28(10):1194-1200.
[11]Zhong X, Zhang HY, Tan H, et al. Association of serum omentin-1 levels with coronary artery disease[J]. Acta Pharmacol Sin,2011,32(7):873-878.
[12]Hye Jin Yoo, Soon Young Hwang, Ho Cheol Hong, et al. Association of circulating omentin-1 level with arterial stiffness and carotid plaque in type 2 diabetes[J].Cardiovasc Diabetol,2011,10:103. Published online 2011 November 22. doi:10.1186/1475-2840-10-103.
[13]Pan H Y, Guo L, Li Q. Changes of serum omentin-1 levels in normal subjects and in patients with impaired glucose regulation and with newly diagnosed and untreated type 2 diabetes[J]. Diabetes Res Clin Pract,2010,88(1):29-33.
[14]Schaffler A, Zeitoun M, Wobser H. Frequency and significance of the novel single nucleotide missense polymorphism Val109Asp in the human gene encoding omentin in Caucasian patients with type 2 diabetes mellitus or chronic inflammatory bowel diseases[J]. Cardiovascular Diabetology,2007,6:3.DOI:10.1186/1475-2840-6-3.
[15]R.C. Cai, L. Wei, J.Z. Di, et al. Expression of omentin in adipose tissues in obese and type 2 diabetic patients[J]. Zhonghua Yi Xue Za Zhi,2009,89:381-384.
[16]李晓红.2型糖尿病并急性心肌梗死的临床分析[J].中原医刊,2008,35(8)：56-57.
[17]Yamawaki H,Tsubaki N,Mukohda M,et al.Omentin,a novel adipokine induces vasodilation in rat isolated blood cessels[J]. Biochem Biophys Res Commun,2010,393(4):668-672.
[18]毕艳,朱大龙,等.网膜素的研究进展[J].中国糖尿病杂志,2007,15(9),574-575.
[19]陈茜,牛建民,等,脂肪细胞因子网膜素-1与代谢综合征[J].实用医学杂志,2011,27(10)：1887-1889.
[20]Maruyama S, Shibata R, Kikuchi R, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism[J]. J Biol Chem,2012,287(1):408-417.
[21]Shang FJ, Wang JP, Liu XT, et al. Serum omentin-1 levels are inversely associated with the presence and severity of coronary artery disease in patients with metabolic syndrome[J]. Biomarkers,2011,16(8):657-662.
[22]Shibata R, Ouchi N, Kikuchi R, et al. Circulating omentin is associated with coronary artery disease in men[J]. Atherosclerosis,2011,219(2):811-814.
[23]Yamawaki H, Kuramoto J, Kameshima S, et al. Omentin, a novel adipocytokine inhibits TNF-induced vascular inflammation in human endothelial cells[J]. Biochem Biophys Res Commun,2011,408(2):339-343.
[24]Xie H, Xie PL, Wu XP, et al. Omentin-1 attenuates arterial calcification and bone loss in osteoprotegerin-deficient mice by inhibition of RANKL expression [J]. Cardiovasc Res, 2011,92(2):296-306.
[25]Maruyama S, Shibata R, Kikuchi R, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism[J]. J Biol Chem,2012,287(1):408-417.
[26]Yamawaki H.Vascular effects of novel adipocytokines:focus on vascular contractility and inflammatory responses [J]. Biol Pharm Bull,2011,34(3):307-310.
[27]B.K. Tan, R. Adya, S. Farhatullah, et al. Omentin-1, a novel adipokine, is decreased in overweight insulin resistant women with the polycystic ovary syndrome:ex vivo and in vivo regulation of omentin-1 by insulin and glucose[J]. Diabetes,2008,57:801-808.
[1]Rabe K, Lehrke M, Parhofer KG, et al. Adipokines and insulin resistance[J].Mol Med, 2008,14(11-12):741-751.
[2]Yang RZ, Xu A, Pray J, et al. Cloning of omentin, a new adipocytokine from omental fat tissue in human[J]. Diabetes,2005,52(suppll):A1.
[3]B.K. Tan, R. Adya, S. Farhatullah, et al. Omentin-1, a novel adipokine, is decreased in overweight insulin resistant women with the polycystic ovary syndrome:ex vivo and in vivo regulation of omentin-1 by insulin and glucose[J]. Diabetes,2008,57:801-808.
[4]De Souza Batista CM, Yang RZ, Lee MJ, et,al. Omentin plasma lecels and gene expression are decreased in obesity[J]. Diabetes,2007,56(6):1655-1661.
[5]Yang RZ, Lee MJ, Hu H, et al. Identification of omentin as a novel depot-specific adipokine in human adipose tissue:possible role in modulating insulin action[J]. Am J Physiol Endocrinol Metab,2006,290:E1253-1261.
[6]毕艳,朱大龙,龚大为.网膜素的研究进展[J].中国糖尿病杂志,2007,15(9)：574-575.
[7]Schaffler A, Neumeier M, Herfarth H, et al. Genomic structure of human omentin, a new adipocytokine expressed in omental adipose tissue[J]. Bilchim Biophys Acta,2005,1732: 96-102.
[8]Fain J N, Sacks H S,Buehrer B, et al. Identification of omentin mRNAin human epicaedial adipose tissue comparison to omentin in subcutaneous, internal mammary artery periadventitial and cisceral abdominal depots[J].Int J Obes,2008,32(5):810-815.
[9]Tsuji S, Uehori J, Matsumoto M, et al. Human intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell wall[J]. J Biol Chem, 2001,276:23456-23463.
[10]Pan H Y,Guo L, Li Q. Changes of serum omentin-1 levels in normal subjects and in patients with impaired glucose regulation and with newly diagnosed and untreated type 2 diabetes[J]. Diabetes Res Clin Pract,2010,88(1):9-33.
[11]Yoon J C, Chickering T W, Rosen E D, et al. Peroxisome proliferator-activated receptor gamma target gene encoding a novel angiopoietin-related protein associated with adipose differentiation[J]. Mol Cell Biol,2000,20:5343-5349.
[12]陈茜,牛建民,温济英,等.脂肪细胞因子网膜素-1与代谢综合征[J].实用医学杂志,2011,27(10)：1887-1889.
[13]Lee JK, Sxhnee J,Pang M, et al. Human homologs of the Xenopus oocyte cortical granule lectin XL35[J]. Glycobilogy,2001,11:65-73.
[14]Suzuki YA, Shin K, Lonnerdal B. Molecular cloning and fuctional expression of a human intestinal lactoferrin receptor[J]. Biochemistry,2001,40:15771-15779.
[15]J.M. Moreno-Navarrete, V. Catalan, F. Ortega, et al.Circulating omentin concentration increases after weight loss[J]. Nutr Metab (Lond),2010,7:27
[16]T.Komiya, Y. Tanigawa, S. Hirohashi. Cloning of the novel gene intelectin, which is expressed in intestinal Paneth cells in mice[J]. Biochem Biophys Res Commun,1998,251: 759-762.
[17]Cantarini L, Simonini G, Fioravanti A, et al. Circulating levels of the adipokines vaspin and omentin in patients with juvenile idiopathic arthritis, and relation to disease activity[J]. Clin Exp Rheumatol,2011,29(6):1044-1048.
[18]Tan BK, Adya R, Syed F,et al, Metformin trement may increase omentin-1 levels in women with polycystic ovary syndrome[J]. Diabetes,2010,59:3023-2031.
[19]Bee K. Tan. Raghu Adya, HArpal S. Randeva. Omentin:A novel link between inflammation, diabesity, and cardiovascular disease[J]. Trends Cardiocasc Med,2010, 20(5):143-148.
[20]Gustafson B. Adipose tissue,inflammation and atherosclerosis [J]. J Atheroscler Thromb, 2010,17(4):332-341.
[21]Orr A.W., Hastings N.E., Blackman B.R., et al. Complex regulation and function of the inflammatory smooth muscle cell phenotypa in atherosclerosis [J]. J Vasc Res,2010,47(2): 168-180.
[22]Yamawaki H, Tsubaki N, Mukohda M, et al. Omentin,a novel adipokine,induces vasodilation in rat isolated blood[J]. Biochem Biophys Res Commun,2010,393(4):668-672.
[23]Yamawaki H.Vascular effects of novel adipocytokines:focus on vascular contractility and inflammatory responses[J]. Biol Pharm Bull,2011,34(3):307-310.
[24]Xie H, Xie PL, Wu XP, et al. Omentin-1 attenuates arterial calcification and bone loss in osteoprotegerin-deficient mice by inhibition of RANKL expression[J]. Cardiovasc Res, 2011,92(2):296-306.
[25]Xie H, Xie PL, Luo XH, et al. Omentin-1 exerts bone-sparing effect in ovariectomized mice[J]. Osteoporos Int,2012,23(4):1425-1436.
[26]El-Mesallamy HO, El-Derany MO, Hamdy NM. Serum omentin-1 and chemerin levels are interrelated in patients with Type 2 diabetes mellitus with or without ischaemic heart disease[J]. Diabet Med,2011,28(10):1194-1200.
[27]R.C. Cai, L. Wei, J.Z. Di, et al. Expression of omentin in adipose tissues in obese and type 2 diabetic patients[J]. Zhonghua Yi Xue Za Zhi,2009,89:381-384.
[28]项守奎,冯文焕,毕艳,等.超重及肥胖人群血清网膜素-1水平的变化[J].中国糖尿病杂志,2009(1)：39-42.
[29]Wurm S, Neumeier M, Weigert J, et al. Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults[J]. Cardiovasc Diabetol,2007,20:6-7.
[30]Akbarzadeh S, Nabipour I, Assadi M, et al. The normoglycemic first-degree relatives of patients with type 2 diabetes mellitus have low circulating omentin-1 and adiponectin levels[J]. Cytokine,2012,58(2):295-299.
[31]Choi JH, Rhee EJ, Kim KH, et al. Plasma omentin-1 levels are reduced in non-obese women with normal glucose tolerance and polycystic ovary syndrome[J]. Eur J Endocrinol. 2011,165(5):789-796.
[32]Yan P, Li L, Yang M, et al. Effects of the long-acting human glucagon-like peptide-1 analog liraglutide on plasma omentin-1 levels in patients with type 2 diabetes mellitus[J]. Diabetes Res Clin Pract,2011,92(3):368-374.
[33]A. Saremi, M. Asghari, A. Ghorbani. Effects of aerobic training on serum omentin-1 and cardiometabolic risk factors in overweight and obese men[J]. J Sports Sci,2010,4:1-6
[34]Sacks HS, Fain JN. Human epicaedial adipose tissue:a reciew[J]. Am Heart J,2007,153: 907-917.
[35]M. Montagnani, L.V. Ravichandran, H. Chen, et al. Insulin receptor substrate-1 and phosphoinositide-dependent kinase-1 are required for insulin-stimulated production of nitric oxide in endothelial cells[J]. Mol Endocrinol,2002,16:1931-1942
[36]Maruyama S, Shibata R, Kikuchi R, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism[J]. J Biol Chem,2012,287(1):408-417.
[37]Shang FJ, Wang JP, Liu XT, et al. Serum omentin-1 levels are inversely associated with the presence and severity of coronary artery disease in patients with metabolic syndrome[J]. Biomarkers,2011,16(8):657-662.
[38]Shibata R, Takahashi R, Kataoka Y, et al. Association of a fat-derived plasma protein omentin with carotid artery intima-media thickness in apparently healthy men[J]. Hypertens Res,2011,34(12):1309-1312.
[39]Shibata R, Ouchi N, Kikuchi R, et al. Circulating omentin is associated with coronary artery disease in men[J]. Atherosclerosis,2011,219(2):811-814.
[40]许曼音,陆广华,陈名道.糖尿病学[M].上海：上海科学技术出版社,2010.471-479.
[41]赵迪,李华,杨再刚,等.内脂素及肿瘤坏死因子-α与2型糖尿病大血管并发症的关系[J].山东医药,2010,50(17)：48-50.
[42]Fu M, Gong DW, Damcott C, et al. Systematic analysis of omentin 1 and omentin 2 on 1q23 as candidate genes for type 2 diabetes in the Old Order Amish (Abstract) [J]. Diabetes, 2004,53:A59.