矿区周边重金属污染农田发展桑树种植产业的可行性研究
|
摘要
本文为研究矿业污染耕地种桑养蚕利用模式的可行性,在广西环江矿业污染区开展了桑地土壤污染特征、桑叶重金属含量和品质调查,着重研究污染桑地土壤和桑叶重金属含量的关系、土壤重金属含量对桑叶品质的影响以及污染桑叶的生物毒性。主要研究结果如下:
1.广西环江污染桑地(受携带尾矿的洪水淹没的)重金属、酸污染严重,污染桑地土壤Pb、Zn、As含量分别是对照桑地(污染桑地附近的未受洪水淹没的)的17.2、3.8、3.5倍,铅、锌、砷含量超标率分别为61.7%、58.8%、47.1%(《土壤环境质量标准》二级);污染桑地在酸性修复之前土壤极度酸化,虽然经初步酸性修复,但是还有近三分之一的污染桑地为强酸性土壤(pH＜4.5)。
2.桑树耐酸性能力强,能在强酸性土壤上生长;桑树也比较耐土壤铅、锌、砷复合污染,能在铅、锌、砷含量分别高达734、1194、53 mg/kg的污染土壤上生长而没有在外观上表现出明显的受胁迫现象。
3.污染老桑叶的Pb、Zn、As和土壤相应重金属含量显著相关;污染桑叶的Pb、Zn含量均显著高于对照桑叶,污染桑叶与对照桑叶的As含量差异不显著;污染桑叶对Pb、Zn、As的富集能力与普通植物相当。
4.桑叶Pb、Zn、As的积累量随桑叶生长周期的延长而增加;在污染桑地,老桑叶(5-8叶位)中的Pb、Zn、As与嫩桑叶(4叶位以上)中相应重金属含量均显著相关;在对照桑地,老桑叶的Zn、As含量与嫩桑叶中相应重金属含量均显著相关,而Pb更易被嫩桑叶富集。
5.污染桑地的桑叶品质没有受到污染物的破坏,污染老桑叶的蛋白质含量反而显著高于未污染老桑叶的。污染桑叶的可溶性蛋白质含量的增高与桑叶重金属含量不相关,与土壤pH值负相关。土壤酸度影响到桑树植株体内的酸度,酸环境有助于可溶性蛋白质含量的增加。
6.污染桑叶重金属含量对蚕的生物毒性小。蚕对污染桑叶和对照桑叶的食下率、消化率无显著差异;污染桑叶和对照桑叶喂养的蚕结茧情况虽然表现为它们的上茧个数、下茧个数存在显著性差异,但是它们的上茧重量并没有达到显著性差异,蚕茧茧层率亦无显著性差异。
7.污染桑叶喂养的蚕吐的蚕丝重金属含量均在《生态纺织品技术要求(GBT18885-2002)》标准限量之内;污染桑叶喂养的蚕的蚕粪Pb、Zn、As含量显著高于对照桑叶喂养的蚕的蚕粪,但远低于《农用污泥中污染物控制标准(GB4284-84)》中相关重金属的限量;蚕蛹的重金属含量也均在《饲料卫生标准(GB3078-2001)》限量之内。
8.桑树耐土壤重金属毒性较强,桑叶重金属的生物毒性小,养蚕产物生态安全,养蚕经济效益高,因此种桑养蚕是矿业污染耕地的合理利用模式。
In order to study the possibility of mulberry-sericulture model applied in mining pollution farmland, a field investigation of soil pollution characteristic, heavy metal content and quality of mulberry leaf was conducted in mulberry land of mining pollution area in Huanjiang county of Guangxi province. The thesis mainly studied the heavy metal content relation between soil and mulberry leaf, the quality of mulberry leaf effected by polluted soil, the bio-toxicity of polluted mulberry leaf. The main result can be summarized as follows:
1) The soil heavy metal and acid pollution of contaminated mulberry land( submerged by flood with tailings) was severe in Guangxi Huanjiang. The content of Pb, Zn and As in contaminated soil was respectively 17.2, 3.8 and 3.5 times as the content of Pb, Zn and As in control soil( not submerged by flood with tailings, near polluted land). The over standard rate of Pb, Zn and As content was respectively 61.7%, 58.8% and 47.1%("Environmental quality standard for soils",Ⅱ). The polluted soil acidified extremely before acidity remediation. After primary acidity remediation, there was still about one third polluted land which was strong acid soil (pH<4.5).
2) Mulberry had a strong resistance of acid and toxicity of Pb, Zn and As in soil. Mulberry could grow normally in contaminated soil with 734mg/kg Pb, 1194mg/kg Zn, 53mg/kg As.
3) The contents of Pb, Zn and As in the old mulberry leaf and the content of corresponding heavy metals in soil were significantly related. Pb and Zn content of pollution leaf were significant higher than the control leaf(p<0.01). But As content of pollution leaf and control leaf had no significant difference. The accumulation Pb, Zn and As ability of polluted mulberry leaf was nearly equivalent to normal plants.
4) The accumulation of Pb, Zn and As in mulberry leaf increased with the. leaf growth cycle prolonging. The content of Pb, Zn and As between old leaf(5-8 leaf position) and tender leaf(above 4 leaf position) had significant correlation in polluted mulberry land. In unpolluted land, only the content of Zn and As between old leaf and tender leaf showed significant con-elation, but Pb was more easily accumulated by tender leaf.
5) Not only the quality of mulberry leaf were not reduced, but also the protein content of old leaf in pollution land were significantly higher than the control. The soluble protein content and heavy metal content in mulberry leaf didn't show correlation, but the soluble protein content in mulberry leaf and pH in soil showed negative correlation. The soil acidity affected on acidity inside mulberry plant body. Acid environment inside plant body promoted the soluble protein content increasing.
6) The bio-toxicity of heavy metals in polluted mulberry leaf was low. The percentage of food ingestion and digestion between the polluted leaf and control leaf had no significant difference. There was significant difference of the good cocoon number between the polluted leaf and control leaf. But there was not significant difference of the good cocoon weight between the polluted leaf and control leaf. The rate of silk between the polluted leaf and control leaf also had no significant difference.
7) The heavy metal content of silk from silkworm fed with polluted mulberry leaf was lower than the limit of heavy metals content seted by "Technical specification for ecological textiles(GBT18885-2002)". The heavy metal content of faecal from silkworm fed with polluted mulberry leaf was significant higher than the heavy metal content of faecal from silkworm fed with control leaf. But the heavy metal content of faecal from silkworm fed with polluted leaf was lower than the limit of heavy metals content seted by "Control standards for pollutants in sludges from agricultural use (GB4284-84)". The heavy metal content of silkworm chrysalis was lower than the limit of heavy metals content seted by "Hygienical standard for feeds(GB3078-2001)".
8). To sum up, mulberry-sericulture is the model of the rational utilization of heavy metal polluted farmland.
1.广西环江污染桑地(受携带尾矿的洪水淹没的)重金属、酸污染严重,污染桑地土壤Pb、Zn、As含量分别是对照桑地(污染桑地附近的未受洪水淹没的)的17.2、3.8、3.5倍,铅、锌、砷含量超标率分别为61.7%、58.8%、47.1%(《土壤环境质量标准》二级);污染桑地在酸性修复之前土壤极度酸化,虽然经初步酸性修复,但是还有近三分之一的污染桑地为强酸性土壤(pH＜4.5)。
2.桑树耐酸性能力强,能在强酸性土壤上生长;桑树也比较耐土壤铅、锌、砷复合污染,能在铅、锌、砷含量分别高达734、1194、53 mg/kg的污染土壤上生长而没有在外观上表现出明显的受胁迫现象。
3.污染老桑叶的Pb、Zn、As和土壤相应重金属含量显著相关;污染桑叶的Pb、Zn含量均显著高于对照桑叶,污染桑叶与对照桑叶的As含量差异不显著;污染桑叶对Pb、Zn、As的富集能力与普通植物相当。
4.桑叶Pb、Zn、As的积累量随桑叶生长周期的延长而增加;在污染桑地,老桑叶(5-8叶位)中的Pb、Zn、As与嫩桑叶(4叶位以上)中相应重金属含量均显著相关;在对照桑地,老桑叶的Zn、As含量与嫩桑叶中相应重金属含量均显著相关,而Pb更易被嫩桑叶富集。
5.污染桑地的桑叶品质没有受到污染物的破坏,污染老桑叶的蛋白质含量反而显著高于未污染老桑叶的。污染桑叶的可溶性蛋白质含量的增高与桑叶重金属含量不相关,与土壤pH值负相关。土壤酸度影响到桑树植株体内的酸度,酸环境有助于可溶性蛋白质含量的增加。
6.污染桑叶重金属含量对蚕的生物毒性小。蚕对污染桑叶和对照桑叶的食下率、消化率无显著差异;污染桑叶和对照桑叶喂养的蚕结茧情况虽然表现为它们的上茧个数、下茧个数存在显著性差异,但是它们的上茧重量并没有达到显著性差异,蚕茧茧层率亦无显著性差异。
7.污染桑叶喂养的蚕吐的蚕丝重金属含量均在《生态纺织品技术要求(GBT18885-2002)》标准限量之内;污染桑叶喂养的蚕的蚕粪Pb、Zn、As含量显著高于对照桑叶喂养的蚕的蚕粪,但远低于《农用污泥中污染物控制标准(GB4284-84)》中相关重金属的限量;蚕蛹的重金属含量也均在《饲料卫生标准(GB3078-2001)》限量之内。
8.桑树耐土壤重金属毒性较强,桑叶重金属的生物毒性小,养蚕产物生态安全,养蚕经济效益高,因此种桑养蚕是矿业污染耕地的合理利用模式。
In order to study the possibility of mulberry-sericulture model applied in mining pollution farmland, a field investigation of soil pollution characteristic, heavy metal content and quality of mulberry leaf was conducted in mulberry land of mining pollution area in Huanjiang county of Guangxi province. The thesis mainly studied the heavy metal content relation between soil and mulberry leaf, the quality of mulberry leaf effected by polluted soil, the bio-toxicity of polluted mulberry leaf. The main result can be summarized as follows:
1) The soil heavy metal and acid pollution of contaminated mulberry land( submerged by flood with tailings) was severe in Guangxi Huanjiang. The content of Pb, Zn and As in contaminated soil was respectively 17.2, 3.8 and 3.5 times as the content of Pb, Zn and As in control soil( not submerged by flood with tailings, near polluted land). The over standard rate of Pb, Zn and As content was respectively 61.7%, 58.8% and 47.1%("Environmental quality standard for soils",Ⅱ). The polluted soil acidified extremely before acidity remediation. After primary acidity remediation, there was still about one third polluted land which was strong acid soil (pH<4.5).
2) Mulberry had a strong resistance of acid and toxicity of Pb, Zn and As in soil. Mulberry could grow normally in contaminated soil with 734mg/kg Pb, 1194mg/kg Zn, 53mg/kg As.
3) The contents of Pb, Zn and As in the old mulberry leaf and the content of corresponding heavy metals in soil were significantly related. Pb and Zn content of pollution leaf were significant higher than the control leaf(p<0.01). But As content of pollution leaf and control leaf had no significant difference. The accumulation Pb, Zn and As ability of polluted mulberry leaf was nearly equivalent to normal plants.
4) The accumulation of Pb, Zn and As in mulberry leaf increased with the. leaf growth cycle prolonging. The content of Pb, Zn and As between old leaf(5-8 leaf position) and tender leaf(above 4 leaf position) had significant correlation in polluted mulberry land. In unpolluted land, only the content of Zn and As between old leaf and tender leaf showed significant con-elation, but Pb was more easily accumulated by tender leaf.
5) Not only the quality of mulberry leaf were not reduced, but also the protein content of old leaf in pollution land were significantly higher than the control. The soluble protein content and heavy metal content in mulberry leaf didn't show correlation, but the soluble protein content in mulberry leaf and pH in soil showed negative correlation. The soil acidity affected on acidity inside mulberry plant body. Acid environment inside plant body promoted the soluble protein content increasing.
6) The bio-toxicity of heavy metals in polluted mulberry leaf was low. The percentage of food ingestion and digestion between the polluted leaf and control leaf had no significant difference. There was significant difference of the good cocoon number between the polluted leaf and control leaf. But there was not significant difference of the good cocoon weight between the polluted leaf and control leaf. The rate of silk between the polluted leaf and control leaf also had no significant difference.
7) The heavy metal content of silk from silkworm fed with polluted mulberry leaf was lower than the limit of heavy metals content seted by "Technical specification for ecological textiles(GBT18885-2002)". The heavy metal content of faecal from silkworm fed with polluted mulberry leaf was significant higher than the heavy metal content of faecal from silkworm fed with control leaf. But the heavy metal content of faecal from silkworm fed with polluted leaf was lower than the limit of heavy metals content seted by "Control standards for pollutants in sludges from agricultural use (GB4284-84)". The heavy metal content of silkworm chrysalis was lower than the limit of heavy metals content seted by "Hygienical standard for feeds(GB3078-2001)".
8). To sum up, mulberry-sericulture is the model of the rational utilization of heavy metal polluted farmland.
引文
蔡铞辉,吴明光,汪雄武,等.广东大宝山多金属矿山环境污染问题及启不.华南地质与矿产,2005,(4):50-54
曾清如,廖铭长,等.郴县东西河流域重金属污染农田的防治技术和生态利用模式.农业环境保护,2002,21(5):428-431
崔德杰,张玉龙.土壤重金属污染现状与修复技术研究进展.土壤通报,2004,35(3):366-370
陈朝明,龚惠群,王凯荣.Cd对桑叶品质,生理生化特性的影响及其机理研究.应用生态学报,1996,7(4):417-423
陈朝明,龚惠群.桑—蚕系统中镉的吸收,累积与迁移.生态学,1999,19(5):664-669
陈默君,贾慎修.中国饲用植物.北京:中国农业出版社,2002,1018
陈同斌,范稚莲,等.磷对超富集植物蜈蚣草吸收砷的影响及其科学意义.科学通报,2002,47(15):1156-1159
戴建一,朱祥瑞.桑叶桑蚕幼虫及茧壳重金属含量的分析测定.蚕桑通报,2004,35(4):20-22
董立军.德兴铜矿生产废水的治理.世界采矿快报,2000,16(10-11):362-363
葛顺楠,宁北芳,丁劲,等.镍致癌机制的研究进展.肿瘤学杂志,1997,13(1):74-77
龚惠群,陈朝明,王凯荣,等.镉对桑蚕生长发育和蚕茧质量影响的研究.应用与环境生物学报,1998,4(2):159-162
管竞芳,潘如圭,徐风梅.大气苯酚对蚕桑污染毒性.农村生态环境(学报),1994,10(2):19-21
赫秀珍,周东美.金属尾矿砂的改良和植被重建研究进展.土壤(Soils),2005,37(1):13-19
黄昀,李道高,刘光德,等.三峡库区柑橘对土壤重金属吸收富集特征研究.中国环境监测,2005.21(1):12-15
黄尚彪.环江桑蚕示范区通过国家级农业标准化验收:http://news.gxradio.com/news/2007/1107/bgxsy/111829.htm,2007
黄自然,杨军,吕雪娟.桑树作为动物饲料的应用价值与研究进展.蚕业科学,2006,32(3):377-385
莫嘉凌.广西蚕桑业发展趋势分析及对策.广西蚕业,2005,42(1):38-43
郦民中,邹晓强.环境污染与养蚕安全.江苏蚕业,2006,(4):15-16
李天煜.我国采矿废弃地生态恢复事业问题剖析.有色金属(矿山部分),2003,55(6):45-48
林初夏,卢文洲,吴永贵,等.大宝山矿水外排的环境影响:Ⅱ.农业生态系统.生态环境,2005. 14(2):169-172
林初夏,龙新宪,童晓立,等.广东大宝山矿区生态环境退化现状及治理途径探讨.生态科学,2003,22(3):205-208
刘庆信,张万英.蚕桑生产中氟化物污染研究进展.山东农业大学学报,1994,25(4):24-29
刘艳青.广西环江沿岸农田土壤重金属污染成因初探:[学位论文]北京:中国科学院研究生院,2007
陆尚旭.河池地区江河水质趋势分析及其污染防治对策.广西水利水电,1999 4):39-41,46
陆尚旭.河池地区江河水质污染现状评价及防治对策.水资源保护,2004,20(2):41-43
陆原.广西桑蚕业坐上全国第一把交椅被称为“广西现象”:http://www.csh.gov.cn/xxlr1_71392.html,2007
鲁如坤.土壤农业化学分析方法.北京:中国农业科技出版社,1999,13-14
楼程富.桑实生苗对镍元素的吸收和迁移.江苏蚕业,1993,(1):1-4
楼程富,本间慎.Ni在桑细胞内的分布及其结合物的分离.浙江农业大学学报,1995,21(1):85-88
罗日杰.环江桑蚕已成县域经济优势产业:http://gxrb.gxnews.com.cn/html/2006-10/27/content_18102.htm,2006
宁树才.马钢南山矿酸性废水的治理及改进.金属矿山,2000,(6):43-45
鲍负,常前发.尾矿利用是实现矿业可持续发展的重要途往.矿业快报,2000,(7):3-5
孙健,铁柏清,钱湛,等.湖南省有色金属矿区重金属污染土壤的植物修复.中南林学院学报,2006,26(1):125-128
宋书巧,吴浩东,蓝唯源.土壤锑污染对桑树的影响初探.资源开发与市场,2008,24(1):1-3,88
万飞.镉对桑蚕生长发育及茧质影响的试验初报.中国蚕业,2004,25(04):23-24
王亚平,王苏明.矿山固体废物的环境地球化学研究进展.矿产综合利用,1998,(3):30-34
王凯荣,陈朝明.镉污染农田农业生态整治与安全高效利用模式.中国环境科学,1998,18(2):97-101
王鸣刚,任小换,刘晓风.植物修复重金属污染土壤的机理及其应用前景.甘肃农业大学学报,2007,42(5):108-113
韦细姣.对大环江上游河段污染物总量控制的探讨.广西水利水电,2001 4):34-36
韦朝阳,陈同斌.重金属超富集植物及植物修复技术研究进展.生态学报,2001,(07)
荆旭慧,李恋卿,潘根兴.不同环境下土壤作物系统中重金属元素迁移分配特点.生态环境,2007,(03):120-124
徐凤平,周兴龙,胡天喜.国内尾矿资源综合利用的现状及建议.矿业快报,2007,23(3):4-6
许乃政,袁旭音,陶于祥.硫多金属矿床开采对水环境的影响——以福建大田地区矿产开发为例.地质通报,2003,22(9):718-724
杨惠芬,李明元,文沈.食品卫生理化检验标准手册.北京:中国标准出版社,1998,103-113
阳正熙.矿区酸性废水的成因及其防治.世界采矿快报,1999,15(10):42-51
余章机,黄时宪.锌元素对桑蚕生长和蚕茧品质的影响.西南农业学报,1997,10(2):81-84
叶志毅,刘红.利用桑树叶资源发展畜牧业生产的可行性分析.中国畜牧杂志,2003,39(1):43-44
增井博之.关于重金属对无菌蚕毒性的研究.江苏蚕业,1996,(3):57-61
翟丽梅.矿业活动密集区农田土壤重金属的污染特征-以广西西江流域为例:[学位论文]北京:中国科学院研究生院,2007
中国农业科学院蚕业研究所.桑蚕.北京:科学出版社,1980,36-39
周连碧.我国矿区土地复垦与生态重建的研究与实践.有色金属,2007,59(2):90-94
周生贤.在全国土壤污染状况调查工作视频会议上的讲话.中国环境监测,2006,22(4):1-2
周伟.镉和铅污染土壤对桑树生长的影响.蚕业科学,1995,21(4):265-266
周伟,尹文胜.汞,砷,铬,铅等无机环境污染物对家蚕的为害.蚕业科学,1991,17(2):109-111
朱玉芳,吴康,崔勇华,等.镉铅对家蚕茧质及血细胞DNA损伤的影响.农业环境保护,2002,21(06):502-504
张晓梅,任发政,葛克山.饲料中添加桑饲料对蛋鸡生产性能和鸡蛋品质的影响.食品科学,2007,28(3):89-91
钟格梅,唐振柱.我国环境中镉、铅、砷污染及其对暴露人群健康影响的研究进展.环境与健康杂志,2006,23(6):562-565
Alpers C.N.,Blowes D.W.Environmental Geochemistry of Sulfide Oxidation.Columbus:American Chemical Society,1994,752
Clementel R.,Walker D.J.,Roig A.,et al.Heavy metal bioavailability in a soil affected by mineral sulphides contamination following the mine spillage at Aznalc611ar(Spain)Biodegradation,2003,14(3):199-205
Cui Y.J.,Zhu Y.G.,Zhai R.H.,et al.Exposure to metal mixtures and human health impacts in a contaminated area in Nanning China.Environment International,2005,31:784-790
Dill H.,Pollmann H.,Bosecker K.,et al.Supergene mineralization in mining residues of the Matchless cupreous pyrite deposit(Namibia)-a clue to the origin of modern and fossil duricrusts in semiarid climates.Journal of Geochemical Exploration,2002,75(1-3):43-70
Kim J.Y.,Kim K.W.,Lee J.U.,et al.Assessment of As and heavy metalcontaminate on in the vicinity of duckum Au-Ag mine,Korea.Environmental Geochemistry and Health,2002,24:215-227
Knoche D.,Embacher A.,Katzur J.Element dynamics of oak ecosystems on acid-sulphurous mine soils in the Lusatian lignite mining district(Eastern Germany).Landscape and Urban Planning,2000,51(2-4):113-122
Nakagawa T.,Handa S.,Maruyama A.,et al.Distribution and diversity of themophilic sulfate-reducing bacteria within a Cu-Pb-Zn mine(Toyoha,Japan).FEMS Microbiology Ecology,2002,41:199-209
Piamphongsant T.Chronic environmental arsenic poisoning.International Journal of Dermatology,1999,38:401-410
Prince S.,Senthilkumar P.,Subburam V.Mulberry-Silkworm Food Chain-A Templet to Assess Heavy Metal Mobility in Terrestrial Ecosystems.Environmental Monitoring and Assessment,2001,69(3):231-238
Tewari R.K.,Kumar P.,Sharma P.N.Antioxidant responses to enhanced generation of superoxide anion radical and hydrogen peroxide in the copper-stressed mulberry plants.Planta,2006,223:1145-1153
Tucker F.B.,Wang K.X.,Fang J.,et al.Effect of Chromium on Hemolymph Catalase Activity and Cocoon Quality of Two Mulberry Silkworm(Bombyx mori L.)Races.Bulletin of Environmental Contamination and Toxicology,2004,73(3):443-447
Tyler G.,Balsberg Pahlsson A.M.,Bengtsson G.,et al.Heavy metal ecology of terrestrial plants,microorganisms and invertebrates:A review.Water,Air and Soil Pollution.1989,189-225
Wang K.R.,Gong H.,Wang Y.,et al.Toxic Effects of Cadmium on Morus alba L.and Bombyx moril L.Plant and Soil,2004,261(1-2):171-180
曾清如,廖铭长,等.郴县东西河流域重金属污染农田的防治技术和生态利用模式.农业环境保护,2002,21(5):428-431
崔德杰,张玉龙.土壤重金属污染现状与修复技术研究进展.土壤通报,2004,35(3):366-370
陈朝明,龚惠群,王凯荣.Cd对桑叶品质,生理生化特性的影响及其机理研究.应用生态学报,1996,7(4):417-423
陈朝明,龚惠群.桑—蚕系统中镉的吸收,累积与迁移.生态学,1999,19(5):664-669
陈默君,贾慎修.中国饲用植物.北京:中国农业出版社,2002,1018
陈同斌,范稚莲,等.磷对超富集植物蜈蚣草吸收砷的影响及其科学意义.科学通报,2002,47(15):1156-1159
戴建一,朱祥瑞.桑叶桑蚕幼虫及茧壳重金属含量的分析测定.蚕桑通报,2004,35(4):20-22
董立军.德兴铜矿生产废水的治理.世界采矿快报,2000,16(10-11):362-363
葛顺楠,宁北芳,丁劲,等.镍致癌机制的研究进展.肿瘤学杂志,1997,13(1):74-77
龚惠群,陈朝明,王凯荣,等.镉对桑蚕生长发育和蚕茧质量影响的研究.应用与环境生物学报,1998,4(2):159-162
管竞芳,潘如圭,徐风梅.大气苯酚对蚕桑污染毒性.农村生态环境(学报),1994,10(2):19-21
赫秀珍,周东美.金属尾矿砂的改良和植被重建研究进展.土壤(Soils),2005,37(1):13-19
黄昀,李道高,刘光德,等.三峡库区柑橘对土壤重金属吸收富集特征研究.中国环境监测,2005.21(1):12-15
黄尚彪.环江桑蚕示范区通过国家级农业标准化验收:http://news.gxradio.com/news/2007/1107/bgxsy/111829.htm,2007
黄自然,杨军,吕雪娟.桑树作为动物饲料的应用价值与研究进展.蚕业科学,2006,32(3):377-385
莫嘉凌.广西蚕桑业发展趋势分析及对策.广西蚕业,2005,42(1):38-43
郦民中,邹晓强.环境污染与养蚕安全.江苏蚕业,2006,(4):15-16
李天煜.我国采矿废弃地生态恢复事业问题剖析.有色金属(矿山部分),2003,55(6):45-48
林初夏,卢文洲,吴永贵,等.大宝山矿水外排的环境影响:Ⅱ.农业生态系统.生态环境,2005. 14(2):169-172
林初夏,龙新宪,童晓立,等.广东大宝山矿区生态环境退化现状及治理途径探讨.生态科学,2003,22(3):205-208
刘庆信,张万英.蚕桑生产中氟化物污染研究进展.山东农业大学学报,1994,25(4):24-29
刘艳青.广西环江沿岸农田土壤重金属污染成因初探:[学位论文]北京:中国科学院研究生院,2007
陆尚旭.河池地区江河水质趋势分析及其污染防治对策.广西水利水电,1999 4):39-41,46
陆尚旭.河池地区江河水质污染现状评价及防治对策.水资源保护,2004,20(2):41-43
陆原.广西桑蚕业坐上全国第一把交椅被称为“广西现象”:http://www.csh.gov.cn/xxlr1_71392.html,2007
鲁如坤.土壤农业化学分析方法.北京:中国农业科技出版社,1999,13-14
楼程富.桑实生苗对镍元素的吸收和迁移.江苏蚕业,1993,(1):1-4
楼程富,本间慎.Ni在桑细胞内的分布及其结合物的分离.浙江农业大学学报,1995,21(1):85-88
罗日杰.环江桑蚕已成县域经济优势产业:http://gxrb.gxnews.com.cn/html/2006-10/27/content_18102.htm,2006
宁树才.马钢南山矿酸性废水的治理及改进.金属矿山,2000,(6):43-45
鲍负,常前发.尾矿利用是实现矿业可持续发展的重要途往.矿业快报,2000,(7):3-5
孙健,铁柏清,钱湛,等.湖南省有色金属矿区重金属污染土壤的植物修复.中南林学院学报,2006,26(1):125-128
宋书巧,吴浩东,蓝唯源.土壤锑污染对桑树的影响初探.资源开发与市场,2008,24(1):1-3,88
万飞.镉对桑蚕生长发育及茧质影响的试验初报.中国蚕业,2004,25(04):23-24
王亚平,王苏明.矿山固体废物的环境地球化学研究进展.矿产综合利用,1998,(3):30-34
王凯荣,陈朝明.镉污染农田农业生态整治与安全高效利用模式.中国环境科学,1998,18(2):97-101
王鸣刚,任小换,刘晓风.植物修复重金属污染土壤的机理及其应用前景.甘肃农业大学学报,2007,42(5):108-113
韦细姣.对大环江上游河段污染物总量控制的探讨.广西水利水电,2001 4):34-36
韦朝阳,陈同斌.重金属超富集植物及植物修复技术研究进展.生态学报,2001,(07)
荆旭慧,李恋卿,潘根兴.不同环境下土壤作物系统中重金属元素迁移分配特点.生态环境,2007,(03):120-124
徐凤平,周兴龙,胡天喜.国内尾矿资源综合利用的现状及建议.矿业快报,2007,23(3):4-6
许乃政,袁旭音,陶于祥.硫多金属矿床开采对水环境的影响——以福建大田地区矿产开发为例.地质通报,2003,22(9):718-724
杨惠芬,李明元,文沈.食品卫生理化检验标准手册.北京:中国标准出版社,1998,103-113
阳正熙.矿区酸性废水的成因及其防治.世界采矿快报,1999,15(10):42-51
余章机,黄时宪.锌元素对桑蚕生长和蚕茧品质的影响.西南农业学报,1997,10(2):81-84
叶志毅,刘红.利用桑树叶资源发展畜牧业生产的可行性分析.中国畜牧杂志,2003,39(1):43-44
增井博之.关于重金属对无菌蚕毒性的研究.江苏蚕业,1996,(3):57-61
翟丽梅.矿业活动密集区农田土壤重金属的污染特征-以广西西江流域为例:[学位论文]北京:中国科学院研究生院,2007
中国农业科学院蚕业研究所.桑蚕.北京:科学出版社,1980,36-39
周连碧.我国矿区土地复垦与生态重建的研究与实践.有色金属,2007,59(2):90-94
周生贤.在全国土壤污染状况调查工作视频会议上的讲话.中国环境监测,2006,22(4):1-2
周伟.镉和铅污染土壤对桑树生长的影响.蚕业科学,1995,21(4):265-266
周伟,尹文胜.汞,砷,铬,铅等无机环境污染物对家蚕的为害.蚕业科学,1991,17(2):109-111
朱玉芳,吴康,崔勇华,等.镉铅对家蚕茧质及血细胞DNA损伤的影响.农业环境保护,2002,21(06):502-504
张晓梅,任发政,葛克山.饲料中添加桑饲料对蛋鸡生产性能和鸡蛋品质的影响.食品科学,2007,28(3):89-91
钟格梅,唐振柱.我国环境中镉、铅、砷污染及其对暴露人群健康影响的研究进展.环境与健康杂志,2006,23(6):562-565
Alpers C.N.,Blowes D.W.Environmental Geochemistry of Sulfide Oxidation.Columbus:American Chemical Society,1994,752
Clementel R.,Walker D.J.,Roig A.,et al.Heavy metal bioavailability in a soil affected by mineral sulphides contamination following the mine spillage at Aznalc611ar(Spain)Biodegradation,2003,14(3):199-205
Cui Y.J.,Zhu Y.G.,Zhai R.H.,et al.Exposure to metal mixtures and human health impacts in a contaminated area in Nanning China.Environment International,2005,31:784-790
Dill H.,Pollmann H.,Bosecker K.,et al.Supergene mineralization in mining residues of the Matchless cupreous pyrite deposit(Namibia)-a clue to the origin of modern and fossil duricrusts in semiarid climates.Journal of Geochemical Exploration,2002,75(1-3):43-70
Kim J.Y.,Kim K.W.,Lee J.U.,et al.Assessment of As and heavy metalcontaminate on in the vicinity of duckum Au-Ag mine,Korea.Environmental Geochemistry and Health,2002,24:215-227
Knoche D.,Embacher A.,Katzur J.Element dynamics of oak ecosystems on acid-sulphurous mine soils in the Lusatian lignite mining district(Eastern Germany).Landscape and Urban Planning,2000,51(2-4):113-122
Nakagawa T.,Handa S.,Maruyama A.,et al.Distribution and diversity of themophilic sulfate-reducing bacteria within a Cu-Pb-Zn mine(Toyoha,Japan).FEMS Microbiology Ecology,2002,41:199-209
Piamphongsant T.Chronic environmental arsenic poisoning.International Journal of Dermatology,1999,38:401-410
Prince S.,Senthilkumar P.,Subburam V.Mulberry-Silkworm Food Chain-A Templet to Assess Heavy Metal Mobility in Terrestrial Ecosystems.Environmental Monitoring and Assessment,2001,69(3):231-238
Tewari R.K.,Kumar P.,Sharma P.N.Antioxidant responses to enhanced generation of superoxide anion radical and hydrogen peroxide in the copper-stressed mulberry plants.Planta,2006,223:1145-1153
Tucker F.B.,Wang K.X.,Fang J.,et al.Effect of Chromium on Hemolymph Catalase Activity and Cocoon Quality of Two Mulberry Silkworm(Bombyx mori L.)Races.Bulletin of Environmental Contamination and Toxicology,2004,73(3):443-447
Tyler G.,Balsberg Pahlsson A.M.,Bengtsson G.,et al.Heavy metal ecology of terrestrial plants,microorganisms and invertebrates:A review.Water,Air and Soil Pollution.1989,189-225
Wang K.R.,Gong H.,Wang Y.,et al.Toxic Effects of Cadmium on Morus alba L.and Bombyx moril L.Plant and Soil,2004,261(1-2):171-180