达乌里胡枝子品系特性研究
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摘要
以充分挖掘利用我国北方广泛分布的达乌里胡枝子种质资源为目的,以中国农业科学院草原研究所筛选到的达乌里胡枝子不同品系及其相关种和种质为试验材料,在已有研究的基础上,采用生态学、形态学、生理学和育种学相结合的实验技术手段,对达乌里胡枝子不同品系进行了品比试验、区域试验、抗逆性鉴定(抗旱、抗盐),以及种子贮藏蛋白遗传多样性和指纹图谱研究。在抗逆性、遗传多样性和指纹图谱等方面还与胡枝子属的其它相关种和种质进行了比较,从多个层面上揭示了达乌里胡枝子4个品系的品系特性。
1.根据达乌里胡枝子4个品系的品比试验、区域试验,初步筛选出达乌里胡枝子优秀品系2个,即:达乌里胡枝子品系8号和牛枝子品系11号。8号品系的分枝数多,株丛直径大,叶量大,植株田间表现整齐,干草产量和种子产量均最高,分别为2823.75kg/ha和452.25 kg/ha;11号品系枝条长、丛径大,分枝较多,匍匐型贴地生长,干草产量和种子产量分别达2463.75kg/ha和339.75kg/ha,与8号品系相比其生产性能稍差。
2.采用不同浓度梯度的PEG和NaCl分别对达乌里胡枝子4个品系及2个对照种的进行胁迫处理,通过发芽动态、相对发芽率、发芽势、活力指数及半致死浓度综合分析表明:种子萌发期耐旱性和耐盐性顺序均为对照种二色胡枝子13号>品系11号>品系8号>品系9号>品系10号>对照种尖叶胡枝子12号; PEG胁迫的半致死浓度分别为0.84MPa、0.72MPa、0.69MPa、0.66MPa、0.58Mpa和0.41Mpa;NaCl胁迫的半致死浓度分别为5.83%、3.12%、2.6%、1.94%、1.6%和0.78%。
3.对达乌里胡枝子4个品系及其相关种和种质在个体和群体两个水平上进行种子贮藏蛋白提取,对其遗传多样性和聚类结果进行分析表明:13份供试材料总基因多样性为0.2785,遗传分化系数(Gst)为0.6325(>0.51),表明63.25%遗传变异存在于供试材料之间,36.75%存在于各供试材料内部。达乌里胡枝子4个品系的总基因多样性为0.1891,遗传分化系数(Gst)为0.4185(<0.51),表明有41.85%的遗传变异存在于各品系之间,58.15%的遗传变异存在于各品系之内。聚类结果显示:供试的13份材料可以聚为4类,1号和13号聚为第一类;2号和3号聚为第二类;4号和12号聚为第三类;其余7份材料聚为第4类。从聚类图反应的系统进化树来看,达乌里胡枝子各种质之间亲缘关系最近,其次是达乌里胡枝子与其变种牛枝子和无梗达乌里胡枝子。而在种级水平上,达乌里胡枝子与尖叶胡枝子亲缘关系较近,其次是截叶胡枝子,与二色胡枝子亲缘关系最远。
4.品比试验、区域试验和抗逆性鉴定(抗旱、耐盐)试验结果综合分析表明,供试的达乌里胡枝子8号品系主要农艺性状、生产性能最好、基因多样性指数最高,抗旱性和耐盐性仅次于11号品系牛枝子。而11号品系牛枝子抗旱性和耐盐性最强。二者各有所长,均可优先申报品种。
The aim of the study was to well use the germplasm resources of the Lespedeza davurica, which was widely distributed in the north of China. The experimental material was the different lines and the related species and germplasms that were screened by Grassland Research Institute of Chinese Academy of Agricultural Science. On the base of previous studies, using ecology, morphology, physiology and breeding methods, this dissertation did the variety comparative test, regional test, the salt resistance and drought resistance, seed storage protains, genetic diversity and fingerprint in different lines of the Lespedeza davurica. Through compare to the other related species and germplasms on stress resistance, genetic diversity and fingerprint and so on, revealed the lines characteristics of Lespedeza davurica.
1. According to the variety comparative and regional test of four lines of Lespedeza davurica, two outstanding lines of Lespedeza davurica be screened out initially that was line 8 and line 11 . The line 8 has many branches , a big clump diameter , many leaves, and the field performance is more uniform. The hay and seed production is 2823.75 kg/ha, 452.25 kg/ha, respectively. Line11 has long branches , more big clump diameter, many branches, its field performance also better, but its hay and seed production is slightly lower than the line 8.
2. The stress treatment of the 4 lines and 2 contrast varieties were conducted by PEG and NaCl at different concentration gradient.The comprehensive analysis of the germination dynamic, the relative germination percentage, the germination power, vigor index and the half-lethal density indicated, the drought resistance and salt resistance order of the 6 materials as follows: contrast species13 > line 11 > line 8 > line 9 > line 10 > contrast species12. The half-lethal density of PEG is 0.84Mpa, 0.72Mpa, 0.69Mpa, 0.66Mpa, 0.58Mp, 0.41Mpa, respectively. The half-lethal density of the NaCl stress is 5.83%, 3.12%, 2.6%, 1.94%,1.6%, 0.78%, respectively.
3. Extract seed storage proteins of four L. davurica lines and its relative species and germplasms at individual and population levels, analyzed the genetic diversity and result of clusters. The total genetic diversity of 13 materials was 0.2785, genetic differentiation coefficient(Gst) was 0.6325(>0.51), it showed that there were 63.25% genetic variation between the materials, and 36.75% genetic variation in every samples. The total genetic diversity of 4 Lespedeza davurica lines was 0.1891, genetic differentiation coefficient(Gst) was 0.4185(<0.51), it showed that there were 41.85% genetic variation between the lines, and 58.15% genetic variation in every line. Cluster result showed that 13 materials may gather four kinds: 1 and 13 gathered for the first kind; 2 and 3 gathered for the second kind; 4 and 12 gathered for the third kind; other 7 materials(include the four lines) gathered for the 4th kind. The system cladogram which reflect from the genetic distance indicated: lines and germplasms of Lespedeza davurica has the more close sibships.On the species leve, Lespedeza davurica and L. hedysaroides has the more close sibships, next is the L. cuneata, finally is L. bicolor.
4. The comprehensive analysis the variety comparative test, regional test , the salt resistance and drought resistance test result indicated: The line8 of Lespedeza davurica has the best agronomic characters and the field performance, the drought resistance and the salt endurance are only inferior to line11. The line 11 of L. davurica var. has better drought resistance and salt resistance than line8. They both has own distinctive trait, may prior be registered variety.
1.根据达乌里胡枝子4个品系的品比试验、区域试验,初步筛选出达乌里胡枝子优秀品系2个,即:达乌里胡枝子品系8号和牛枝子品系11号。8号品系的分枝数多,株丛直径大,叶量大,植株田间表现整齐,干草产量和种子产量均最高,分别为2823.75kg/ha和452.25 kg/ha;11号品系枝条长、丛径大,分枝较多,匍匐型贴地生长,干草产量和种子产量分别达2463.75kg/ha和339.75kg/ha,与8号品系相比其生产性能稍差。
2.采用不同浓度梯度的PEG和NaCl分别对达乌里胡枝子4个品系及2个对照种的进行胁迫处理,通过发芽动态、相对发芽率、发芽势、活力指数及半致死浓度综合分析表明:种子萌发期耐旱性和耐盐性顺序均为对照种二色胡枝子13号>品系11号>品系8号>品系9号>品系10号>对照种尖叶胡枝子12号; PEG胁迫的半致死浓度分别为0.84MPa、0.72MPa、0.69MPa、0.66MPa、0.58Mpa和0.41Mpa;NaCl胁迫的半致死浓度分别为5.83%、3.12%、2.6%、1.94%、1.6%和0.78%。
3.对达乌里胡枝子4个品系及其相关种和种质在个体和群体两个水平上进行种子贮藏蛋白提取,对其遗传多样性和聚类结果进行分析表明:13份供试材料总基因多样性为0.2785,遗传分化系数(Gst)为0.6325(>0.51),表明63.25%遗传变异存在于供试材料之间,36.75%存在于各供试材料内部。达乌里胡枝子4个品系的总基因多样性为0.1891,遗传分化系数(Gst)为0.4185(<0.51),表明有41.85%的遗传变异存在于各品系之间,58.15%的遗传变异存在于各品系之内。聚类结果显示:供试的13份材料可以聚为4类,1号和13号聚为第一类;2号和3号聚为第二类;4号和12号聚为第三类;其余7份材料聚为第4类。从聚类图反应的系统进化树来看,达乌里胡枝子各种质之间亲缘关系最近,其次是达乌里胡枝子与其变种牛枝子和无梗达乌里胡枝子。而在种级水平上,达乌里胡枝子与尖叶胡枝子亲缘关系较近,其次是截叶胡枝子,与二色胡枝子亲缘关系最远。
4.品比试验、区域试验和抗逆性鉴定(抗旱、耐盐)试验结果综合分析表明,供试的达乌里胡枝子8号品系主要农艺性状、生产性能最好、基因多样性指数最高,抗旱性和耐盐性仅次于11号品系牛枝子。而11号品系牛枝子抗旱性和耐盐性最强。二者各有所长,均可优先申报品种。
The aim of the study was to well use the germplasm resources of the Lespedeza davurica, which was widely distributed in the north of China. The experimental material was the different lines and the related species and germplasms that were screened by Grassland Research Institute of Chinese Academy of Agricultural Science. On the base of previous studies, using ecology, morphology, physiology and breeding methods, this dissertation did the variety comparative test, regional test, the salt resistance and drought resistance, seed storage protains, genetic diversity and fingerprint in different lines of the Lespedeza davurica. Through compare to the other related species and germplasms on stress resistance, genetic diversity and fingerprint and so on, revealed the lines characteristics of Lespedeza davurica.
1. According to the variety comparative and regional test of four lines of Lespedeza davurica, two outstanding lines of Lespedeza davurica be screened out initially that was line 8 and line 11 . The line 8 has many branches , a big clump diameter , many leaves, and the field performance is more uniform. The hay and seed production is 2823.75 kg/ha, 452.25 kg/ha, respectively. Line11 has long branches , more big clump diameter, many branches, its field performance also better, but its hay and seed production is slightly lower than the line 8.
2. The stress treatment of the 4 lines and 2 contrast varieties were conducted by PEG and NaCl at different concentration gradient.The comprehensive analysis of the germination dynamic, the relative germination percentage, the germination power, vigor index and the half-lethal density indicated, the drought resistance and salt resistance order of the 6 materials as follows: contrast species13 > line 11 > line 8 > line 9 > line 10 > contrast species12. The half-lethal density of PEG is 0.84Mpa, 0.72Mpa, 0.69Mpa, 0.66Mpa, 0.58Mp, 0.41Mpa, respectively. The half-lethal density of the NaCl stress is 5.83%, 3.12%, 2.6%, 1.94%,1.6%, 0.78%, respectively.
3. Extract seed storage proteins of four L. davurica lines and its relative species and germplasms at individual and population levels, analyzed the genetic diversity and result of clusters. The total genetic diversity of 13 materials was 0.2785, genetic differentiation coefficient(Gst) was 0.6325(>0.51), it showed that there were 63.25% genetic variation between the materials, and 36.75% genetic variation in every samples. The total genetic diversity of 4 Lespedeza davurica lines was 0.1891, genetic differentiation coefficient(Gst) was 0.4185(<0.51), it showed that there were 41.85% genetic variation between the lines, and 58.15% genetic variation in every line. Cluster result showed that 13 materials may gather four kinds: 1 and 13 gathered for the first kind; 2 and 3 gathered for the second kind; 4 and 12 gathered for the third kind; other 7 materials(include the four lines) gathered for the 4th kind. The system cladogram which reflect from the genetic distance indicated: lines and germplasms of Lespedeza davurica has the more close sibships.On the species leve, Lespedeza davurica and L. hedysaroides has the more close sibships, next is the L. cuneata, finally is L. bicolor.
4. The comprehensive analysis the variety comparative test, regional test , the salt resistance and drought resistance test result indicated: The line8 of Lespedeza davurica has the best agronomic characters and the field performance, the drought resistance and the salt endurance are only inferior to line11. The line 11 of L. davurica var. has better drought resistance and salt resistance than line8. They both has own distinctive trait, may prior be registered variety.
引文
[1]李树刚,杨衔晋,黄普华等编.中国植物志[M].北京:科学出版社,1995,41:131~159.
[2]傅书遐.中国主要植物图说一豆科[M].北京:科学出版社,1955.
[3]陈默君,贾慎修,黄兆华.中国饲用植物志〔第二卷〕[M].北京:农业出版社,1989, 172~177.
[4]中国植物志编写委员会.中国植物志第(四十一卷)[M].北京:中国科学出版社,1999.
[5]大井次三郎(ohwi).日本植物志[M].东京:大同印刷株式会社,1956.
[6]贺士元.北京植物志[M].北京:中国科学出版社,1982.
[7]贺士元,邢其华,尹祖棠等编.北京植物志(上册) [M].北京:北京出版社,1993.462~467.
[8]张月民.胡枝子的育苗及造林技术[J].中国林副特产,1998,(2):27.
[9]孙秀殿,李纯丽,张凤霞.胡枝子的利用与栽培[J].特种经济动植物,1999,(2):33.
[10]吴景才,程润柏等.关于胡枝子开发利用的研究[J].水土保持科技情报,1996,(3):52~53.
[11]马毓泉.内蒙古植物志(第三卷)[M].呼和浩特:内蒙古人民出版社,1983.
[12]夏亦荠.二色胡枝子和达乌里胡枝子若干生物学特性和营养成分的分析[J].草业科学,1990,7(1):9~14.
[13]夏亦荠,苏加楷,熊德邵.二色胡枝子和达乌里胡枝子若干生物学特性和营养成分的分析[J].草业科学,1990,7(1):9~14.
[14]朱志诚,贾东林.达乌里胡枝子群落生物量初步研究[J].中国草原,1994,(3):25~28.
[15]孙启忠,韩建国,桂荣等.科尔沁沙地达乌里胡枝子生物量研究[J].中国草地,2001,23(4):21~26.
[16]张宝田,穆春生,金成吉,张福田.松嫩草地2种胡枝子地上生物量动态及其种间比较[J].草业学报2006,15(3):36~41.
[17]夏富才.松嫩平原三种豆科植物抗盐结构研究[硕士学位论文].2002
[18]夏亦荠等.十种胡枝子核型的研究[J].中国草地,1989,(2).
[19]阎贵兴,云锦凤等.35种国产饲用植物染色体数目的研究[J].中国草地,1991,(6).
[20]阎贵兴.中国草地饲用植物染色体研究[M].呼和浩特:内蒙古人民出版社,2001.
[21]夏亦荠.十种胡枝子的核型及二种胡枝子的若干形态学性状的初步研究[D].北京,1986.
[22]徐泽荣.野生截叶胡枝子生物学特性和经济性状的研究[J].草业学报,1993,2(3):76~80.
[23]张义贤.胡枝子的核型研究[J].山西大学学报(自然科学版),1990,13(1):87~89.
[24] Kawakami J.Chromosome numbers in Leguminosae[J],BMag.Tokyo,1930,44:319~328.
[25] Cooper D C.Chromosome numbers in the Leguminosae[J]Amer.Journ.Bot.,1936,23:231~233.
[26] Lee Y N.Chromosome numbers of flowering plants in Kor[M].Journ.KoreanRes.Inst,1969.
[27] Kondo K.Interspecific variation of karyotypes in some species of Lespedeza and Dismodium[J].La. Chromosome.1977,2(5):123~137.
[28]乌仁其木格,陈海云.胡枝子属牧草种子同工酶的分析[J].内蒙古农牧学院学报,1998,19(1):13~17.
[29]李昌林,陈默君,颜艳.二色胡枝子品种SOD、POD同工酶的酶谱分析[J].草地学报,2003,9:210~213.
[30]张吉宇.14个野生胡枝子居群遗传多样性研究[D].甘肃兰州:甘肃农业大学硕士论文,2003.
[31]李昌林,陈默君,颜艳.二色胡枝子品种SOD. POD下酶的酶谱分析[J].草地学报,2003(3): 210~213.
[32]乌仁其木格,布仁占雅等.胡枝子属牧草种子同工酶的分析[J].内蒙古农牧学院学报,1998(1): 13~17.
[33]周关宇.有关同工酶分析的几个问题[J].植物生理学通讯,1983,(1):1~4.
[34]乌仁其木格.二色胡枝子种子硬实特性的研究[J].内蒙古农牧学院学报,1996,(1).
[35]周永刚,王洪新,胡志昂等.植株内种子蛋白多样性与繁育系统[J].植物学报,2000, 42 (9): 910~912.
[36]胡志昂,王洪新.蛋白质多样性和品种鉴定[J].植物学报,1991, 33(7): 556~564.
[37]兰海燕,李立会.蛋白质凝胶电泳技术在作物品种鉴定中的应用[J].中国农业科学,2002,35(8):916~920.
[38]曾慕衡,杜庭碧,马守才.棉花种子蛋白凝胶电泳方法及其在品种鉴定中的应用[J].陕西农业科学,1996,(2):14~16.
[39]甘莉.油菜种子贮藏蛋白的遗传多样性分析[J].华中农业大学学报,1999,(6):528~532.
[40]郭尧君.蛋白质电泳实验技术[M].北京:科学出版社,2001.
[41]颜廷进.蛋白质电泳技术的改良及其应用效果[J].山东农业科学,1998,(5):19~21
[42]张吉宁,袁庆华等.多花胡枝子基因组DNA提取与RAPD反应体系优化[J].草地学报,2004(3):219~222.
[43]阎伟红.胡枝子属植物野生种质资源遗传多样性研究[D].中国农业科学院草原研究所.2007.
[44] Maximowicz C J. Synopsis generis Lespedeza Michx[M]. Bot. Amer, 1873, 2:329~388.
[45] Pierce W P.Cytogy of the genus Lespedeza[J].Amer.Bot.1939,26:736~744.
[46] Young J O. Cytological investigations in Desmodium and Lepedeza[J]. Bot. Gaz. 1940,101: 839~850.
[47] Clewell A F.The biology of the common native Lespedezas in southern Indiana[J]. Brittonia,1964,16:208~219.
[48] Akiynma S.Taxonomic studies of the Japanese species of the section Macro Lespedeza of the genus Lespedeza(Legumnosae)[M].Tokyo:University of Tokyo,1985.
[49] Donnclly E.D.Selection for chasmogamy in Sericea Lespedeza[J]. Crop Scicnce,1979, 19(4):528~531.
[50]杨瑞丽.植物耐旱性及其机理[J].内蒙古科技与经济,2003,4:107~108.
[51]董晓霞,赵树慧等.苇状羊茅盐胁迫下生理效应的研究[J].草业科学.1998,15(5):10~14.
[52]赵可夫,李法曾主编.中国盐生植物[M].北京:科学出版.1998:1~2.
[53]李瑞云,鲁纯养等.植物耐盐行研究现状与展望[J].盐碱地利用.1989,(1):38~41.
[54]谢振宇,杨光穗.牧草耐盐行研究进展[J].草业科学.2003,20(8):11~17.
[55]刘祖,张石成主编.植物抗性生理学[M].北京:中国农业出版社,1993:248~249.
[56]李树华,许兴等.不同小麦品种对盐碱胁迫的生理及农艺性状的反应[J].麦类作物学报,2000,20(4):63~67.
[57]罗庆云,於丙军等.大豆苗期耐盐行鉴定指标的检验[J].大豆科学,2001,20(3):177~182.
[58]邹志国,潘昌保等.江苏海垦区主要农作物耐盐性实验研究[J].土壤通报,1993,30(1):23~25.
[59]赵可夫著.植物抗盐生理[J].北京:中国科学技术出版社,1993,63~68.
[60]易克贤,陈功.我国亚热带红壤丘陵岗地豆科牧草引进研究[J].草业科,1994,11: 2,15-18.
[61]徐炳声,李学禹,顾德兴等.胡枝子种内地形梯度变异的分析[J].植物分类学报.1983(3):21.
[62]杨艳生,刘柏根等.水土资源恢复中的先锋豆科灌木一胡枝子的栽植研究[J].长江流域资源与环境.1994,3(4) :330~336.
[63]欧阳延生等.美国截叶胡枝子在红壤丘陵岗地的适应性及其应用研究[J].江西农业科技.1996,(2) :44~45.
[64]高琼,陈晓阳,杜金友等.不同种和种源胡枝子的耐旱性差异研究[J].北华大学学报(自然科学版) ,2005.
[65]徐本美,顾增辉.硫酸处理硬实种子的效果[J].植物生理学通报,1985,(2):22~25.
[66]李昌林,陈默君.胡枝子种子萌发及幼苗生长[J].黑龙江畜牧兽医,1994(7):18~21.
[67]谷安琳,Holzworth-L.进行过几种豆科牧草在内蒙古干早草原的建植试验[J].中国草地,1994:(6),12~15.
[68]谷安琳等.几种豆科牧草早作条件下的牧草产量分析[J].中国草地,1998 (5) ;26~30.
[69]奚同行,林圣玉.胡枝子的开发利用价值及发展前景[J].中国水土保持,1995,(4):42~43.
[70]顾振文.胡枝子林的营造管理技术及水土保持效益分析[J].中国水土保持,1996,(5):29~31.
[71]李延安,贾黎明,杨丽.胡枝子应用价值及丰产栽培技术研究[J].河北林果研究,2004,(2):165~169.
[72]王桑基.优质菌材胡枝子的栽培[J].福建农林科技,1999,(增刊):143.
[73]吴景才,程润柏等.关于胡枝子开发利用的研究[J].水土保持科技情报,1996,(3):52~53.
[74]李延交,贾黎明,杨丽等.胡枝子应用价伯及丰产栽培技术研究进展[J].河北林果研究,2004,19(2):185~192.
[75]李延交.饲用型胡枝子引种、筛选及栽培技术研究[D].北京林业大学学位论文,2003.
[76]王平,温玉柱,张晓梅.胡枝子水流调节林带保持水土效益研究[J].黑龙江水利科技,2000,(2):6~7.
[77]刘修圣,吕鹏怀,李辉.胡枝子对水土保持作用的研究[J].黑龙江水专学报,2000,27(2):41~42.
[78]李延安,贾黎明,杨丽等.胡枝子应用价值及丰产栽培技术研究进展[J].河北林果研究,2004,19(2):185~192.
[79] Powell,J.D.Beaty,E.R.Young,W.C.Registration of‘Ambro’virgata Lespedeza(Reg No.7)[J]. Crop science, 1978,18(2): 354.
[80] Henry D.S and Taylo;N .L .Registration of‘Appalow’sericea Lespedeza(Reg No.10)[J].Crop science, 1981,21(1):144.
[81] Owsley,C.M.and Surrcncy,E.D.Registration of‘Amquail’thunberg Lespedeza[J].Crop science,1989,29(1):238.
[82] West . C. P, BeuselinCK , P R , Huneycutt. H . J and McGraw . R L‘Marion’Lespedeza:a new forage cultivar Arkansas farm research[J] . Arkansas Agriculture Experiment Station, 1991, 40(41): 44.
[83] Mosjidis,J.A .Registration of‘AU Grazer’Sericea Lespedeza[J].Crop science,2001,41(1):262.
[84] Donnelly,E.D. and Minton, N. A. Registration of Serala 76 and Interstate 76 sericea Lespedeza[J].Crop science,1979b.19(6):929.
[85]欧阳延生,戴征煌,吴志勇等.美国截叶胡枝子在红壤丘陵岗地的适应性及其应用研究[J].江西农业科技,1996(2):44~45.
[86]Akiynma S.Taxonomic studies of the Japanese species of the section Macro Lespedeza of the genus Lespedeza(Legumnosae)[M]. Tokyo:University of Tokyo, 1985.
[87] Qiu J,MosJidis J A,Williams J C.Variability for temperature of germination in sericea Lespedeza germplasm[J].Crop Science,1995,35(1):237~241.
[88]杜国坚.马尾松林下栽植胡枝子对土壤肥力的影响[J].河北林果研究,1998,13(4):322-327.
[89] Cole C. T and BicsBocr D. D. Monomoxphism,reduced gene flow, and cleistogamy in rare and common species of Lespedeza ( Fnbneene)[J]. American Journal of Botany ,1992, 79(5): 567~575.
[90]曹帮华,吕世海,刘欣玲,武德.羊柴种子抗旱抗盐萌发生理研究[J].中国草地学报,2007,4:33~37.
[91]安守芹,于卓,孔丽娟,陈晓荣.花棒等四种豆科植物种子萌发及苗期耐盐性的研究[J].中国草地,1995,6:29~32.
[92]罗桂花,马继雄,花立民,陈振宁,曾阳,马永贵.青海燕麦农家品种的醇溶蛋白
[93]罗桂花,马基雄,花立民,陈振宁等.青海燕麦农家品种的醇溶蛋白电泳分析[J].草业学报,2004,13(2):l12~l14.
[94]王照兰.苜蓿种质资源评价及优良育种材料的遗传分析[D].呼和浩特:内蒙古农业大学,2003.
[2]傅书遐.中国主要植物图说一豆科[M].北京:科学出版社,1955.
[3]陈默君,贾慎修,黄兆华.中国饲用植物志〔第二卷〕[M].北京:农业出版社,1989, 172~177.
[4]中国植物志编写委员会.中国植物志第(四十一卷)[M].北京:中国科学出版社,1999.
[5]大井次三郎(ohwi).日本植物志[M].东京:大同印刷株式会社,1956.
[6]贺士元.北京植物志[M].北京:中国科学出版社,1982.
[7]贺士元,邢其华,尹祖棠等编.北京植物志(上册) [M].北京:北京出版社,1993.462~467.
[8]张月民.胡枝子的育苗及造林技术[J].中国林副特产,1998,(2):27.
[9]孙秀殿,李纯丽,张凤霞.胡枝子的利用与栽培[J].特种经济动植物,1999,(2):33.
[10]吴景才,程润柏等.关于胡枝子开发利用的研究[J].水土保持科技情报,1996,(3):52~53.
[11]马毓泉.内蒙古植物志(第三卷)[M].呼和浩特:内蒙古人民出版社,1983.
[12]夏亦荠.二色胡枝子和达乌里胡枝子若干生物学特性和营养成分的分析[J].草业科学,1990,7(1):9~14.
[13]夏亦荠,苏加楷,熊德邵.二色胡枝子和达乌里胡枝子若干生物学特性和营养成分的分析[J].草业科学,1990,7(1):9~14.
[14]朱志诚,贾东林.达乌里胡枝子群落生物量初步研究[J].中国草原,1994,(3):25~28.
[15]孙启忠,韩建国,桂荣等.科尔沁沙地达乌里胡枝子生物量研究[J].中国草地,2001,23(4):21~26.
[16]张宝田,穆春生,金成吉,张福田.松嫩草地2种胡枝子地上生物量动态及其种间比较[J].草业学报2006,15(3):36~41.
[17]夏富才.松嫩平原三种豆科植物抗盐结构研究[硕士学位论文].2002
[18]夏亦荠等.十种胡枝子核型的研究[J].中国草地,1989,(2).
[19]阎贵兴,云锦凤等.35种国产饲用植物染色体数目的研究[J].中国草地,1991,(6).
[20]阎贵兴.中国草地饲用植物染色体研究[M].呼和浩特:内蒙古人民出版社,2001.
[21]夏亦荠.十种胡枝子的核型及二种胡枝子的若干形态学性状的初步研究[D].北京,1986.
[22]徐泽荣.野生截叶胡枝子生物学特性和经济性状的研究[J].草业学报,1993,2(3):76~80.
[23]张义贤.胡枝子的核型研究[J].山西大学学报(自然科学版),1990,13(1):87~89.
[24] Kawakami J.Chromosome numbers in Leguminosae[J],BMag.Tokyo,1930,44:319~328.
[25] Cooper D C.Chromosome numbers in the Leguminosae[J]Amer.Journ.Bot.,1936,23:231~233.
[26] Lee Y N.Chromosome numbers of flowering plants in Kor[M].Journ.KoreanRes.Inst,1969.
[27] Kondo K.Interspecific variation of karyotypes in some species of Lespedeza and Dismodium[J].La. Chromosome.1977,2(5):123~137.
[28]乌仁其木格,陈海云.胡枝子属牧草种子同工酶的分析[J].内蒙古农牧学院学报,1998,19(1):13~17.
[29]李昌林,陈默君,颜艳.二色胡枝子品种SOD、POD同工酶的酶谱分析[J].草地学报,2003,9:210~213.
[30]张吉宇.14个野生胡枝子居群遗传多样性研究[D].甘肃兰州:甘肃农业大学硕士论文,2003.
[31]李昌林,陈默君,颜艳.二色胡枝子品种SOD. POD下酶的酶谱分析[J].草地学报,2003(3): 210~213.
[32]乌仁其木格,布仁占雅等.胡枝子属牧草种子同工酶的分析[J].内蒙古农牧学院学报,1998(1): 13~17.
[33]周关宇.有关同工酶分析的几个问题[J].植物生理学通讯,1983,(1):1~4.
[34]乌仁其木格.二色胡枝子种子硬实特性的研究[J].内蒙古农牧学院学报,1996,(1).
[35]周永刚,王洪新,胡志昂等.植株内种子蛋白多样性与繁育系统[J].植物学报,2000, 42 (9): 910~912.
[36]胡志昂,王洪新.蛋白质多样性和品种鉴定[J].植物学报,1991, 33(7): 556~564.
[37]兰海燕,李立会.蛋白质凝胶电泳技术在作物品种鉴定中的应用[J].中国农业科学,2002,35(8):916~920.
[38]曾慕衡,杜庭碧,马守才.棉花种子蛋白凝胶电泳方法及其在品种鉴定中的应用[J].陕西农业科学,1996,(2):14~16.
[39]甘莉.油菜种子贮藏蛋白的遗传多样性分析[J].华中农业大学学报,1999,(6):528~532.
[40]郭尧君.蛋白质电泳实验技术[M].北京:科学出版社,2001.
[41]颜廷进.蛋白质电泳技术的改良及其应用效果[J].山东农业科学,1998,(5):19~21
[42]张吉宁,袁庆华等.多花胡枝子基因组DNA提取与RAPD反应体系优化[J].草地学报,2004(3):219~222.
[43]阎伟红.胡枝子属植物野生种质资源遗传多样性研究[D].中国农业科学院草原研究所.2007.
[44] Maximowicz C J. Synopsis generis Lespedeza Michx[M]. Bot. Amer, 1873, 2:329~388.
[45] Pierce W P.Cytogy of the genus Lespedeza[J].Amer.Bot.1939,26:736~744.
[46] Young J O. Cytological investigations in Desmodium and Lepedeza[J]. Bot. Gaz. 1940,101: 839~850.
[47] Clewell A F.The biology of the common native Lespedezas in southern Indiana[J]. Brittonia,1964,16:208~219.
[48] Akiynma S.Taxonomic studies of the Japanese species of the section Macro Lespedeza of the genus Lespedeza(Legumnosae)[M].Tokyo:University of Tokyo,1985.
[49] Donnclly E.D.Selection for chasmogamy in Sericea Lespedeza[J]. Crop Scicnce,1979, 19(4):528~531.
[50]杨瑞丽.植物耐旱性及其机理[J].内蒙古科技与经济,2003,4:107~108.
[51]董晓霞,赵树慧等.苇状羊茅盐胁迫下生理效应的研究[J].草业科学.1998,15(5):10~14.
[52]赵可夫,李法曾主编.中国盐生植物[M].北京:科学出版.1998:1~2.
[53]李瑞云,鲁纯养等.植物耐盐行研究现状与展望[J].盐碱地利用.1989,(1):38~41.
[54]谢振宇,杨光穗.牧草耐盐行研究进展[J].草业科学.2003,20(8):11~17.
[55]刘祖,张石成主编.植物抗性生理学[M].北京:中国农业出版社,1993:248~249.
[56]李树华,许兴等.不同小麦品种对盐碱胁迫的生理及农艺性状的反应[J].麦类作物学报,2000,20(4):63~67.
[57]罗庆云,於丙军等.大豆苗期耐盐行鉴定指标的检验[J].大豆科学,2001,20(3):177~182.
[58]邹志国,潘昌保等.江苏海垦区主要农作物耐盐性实验研究[J].土壤通报,1993,30(1):23~25.
[59]赵可夫著.植物抗盐生理[J].北京:中国科学技术出版社,1993,63~68.
[60]易克贤,陈功.我国亚热带红壤丘陵岗地豆科牧草引进研究[J].草业科,1994,11: 2,15-18.
[61]徐炳声,李学禹,顾德兴等.胡枝子种内地形梯度变异的分析[J].植物分类学报.1983(3):21.
[62]杨艳生,刘柏根等.水土资源恢复中的先锋豆科灌木一胡枝子的栽植研究[J].长江流域资源与环境.1994,3(4) :330~336.
[63]欧阳延生等.美国截叶胡枝子在红壤丘陵岗地的适应性及其应用研究[J].江西农业科技.1996,(2) :44~45.
[64]高琼,陈晓阳,杜金友等.不同种和种源胡枝子的耐旱性差异研究[J].北华大学学报(自然科学版) ,2005.
[65]徐本美,顾增辉.硫酸处理硬实种子的效果[J].植物生理学通报,1985,(2):22~25.
[66]李昌林,陈默君.胡枝子种子萌发及幼苗生长[J].黑龙江畜牧兽医,1994(7):18~21.
[67]谷安琳,Holzworth-L.进行过几种豆科牧草在内蒙古干早草原的建植试验[J].中国草地,1994:(6),12~15.
[68]谷安琳等.几种豆科牧草早作条件下的牧草产量分析[J].中国草地,1998 (5) ;26~30.
[69]奚同行,林圣玉.胡枝子的开发利用价值及发展前景[J].中国水土保持,1995,(4):42~43.
[70]顾振文.胡枝子林的营造管理技术及水土保持效益分析[J].中国水土保持,1996,(5):29~31.
[71]李延安,贾黎明,杨丽.胡枝子应用价值及丰产栽培技术研究[J].河北林果研究,2004,(2):165~169.
[72]王桑基.优质菌材胡枝子的栽培[J].福建农林科技,1999,(增刊):143.
[73]吴景才,程润柏等.关于胡枝子开发利用的研究[J].水土保持科技情报,1996,(3):52~53.
[74]李延交,贾黎明,杨丽等.胡枝子应用价伯及丰产栽培技术研究进展[J].河北林果研究,2004,19(2):185~192.
[75]李延交.饲用型胡枝子引种、筛选及栽培技术研究[D].北京林业大学学位论文,2003.
[76]王平,温玉柱,张晓梅.胡枝子水流调节林带保持水土效益研究[J].黑龙江水利科技,2000,(2):6~7.
[77]刘修圣,吕鹏怀,李辉.胡枝子对水土保持作用的研究[J].黑龙江水专学报,2000,27(2):41~42.
[78]李延安,贾黎明,杨丽等.胡枝子应用价值及丰产栽培技术研究进展[J].河北林果研究,2004,19(2):185~192.
[79] Powell,J.D.Beaty,E.R.Young,W.C.Registration of‘Ambro’virgata Lespedeza(Reg No.7)[J]. Crop science, 1978,18(2): 354.
[80] Henry D.S and Taylo;N .L .Registration of‘Appalow’sericea Lespedeza(Reg No.10)[J].Crop science, 1981,21(1):144.
[81] Owsley,C.M.and Surrcncy,E.D.Registration of‘Amquail’thunberg Lespedeza[J].Crop science,1989,29(1):238.
[82] West . C. P, BeuselinCK , P R , Huneycutt. H . J and McGraw . R L‘Marion’Lespedeza:a new forage cultivar Arkansas farm research[J] . Arkansas Agriculture Experiment Station, 1991, 40(41): 44.
[83] Mosjidis,J.A .Registration of‘AU Grazer’Sericea Lespedeza[J].Crop science,2001,41(1):262.
[84] Donnelly,E.D. and Minton, N. A. Registration of Serala 76 and Interstate 76 sericea Lespedeza[J].Crop science,1979b.19(6):929.
[85]欧阳延生,戴征煌,吴志勇等.美国截叶胡枝子在红壤丘陵岗地的适应性及其应用研究[J].江西农业科技,1996(2):44~45.
[86]Akiynma S.Taxonomic studies of the Japanese species of the section Macro Lespedeza of the genus Lespedeza(Legumnosae)[M]. Tokyo:University of Tokyo, 1985.
[87] Qiu J,MosJidis J A,Williams J C.Variability for temperature of germination in sericea Lespedeza germplasm[J].Crop Science,1995,35(1):237~241.
[88]杜国坚.马尾松林下栽植胡枝子对土壤肥力的影响[J].河北林果研究,1998,13(4):322-327.
[89] Cole C. T and BicsBocr D. D. Monomoxphism,reduced gene flow, and cleistogamy in rare and common species of Lespedeza ( Fnbneene)[J]. American Journal of Botany ,1992, 79(5): 567~575.
[90]曹帮华,吕世海,刘欣玲,武德.羊柴种子抗旱抗盐萌发生理研究[J].中国草地学报,2007,4:33~37.
[91]安守芹,于卓,孔丽娟,陈晓荣.花棒等四种豆科植物种子萌发及苗期耐盐性的研究[J].中国草地,1995,6:29~32.
[92]罗桂花,马继雄,花立民,陈振宁,曾阳,马永贵.青海燕麦农家品种的醇溶蛋白
[93]罗桂花,马基雄,花立民,陈振宁等.青海燕麦农家品种的醇溶蛋白电泳分析[J].草业学报,2004,13(2):l12~l14.
[94]王照兰.苜蓿种质资源评价及优良育种材料的遗传分析[D].呼和浩特:内蒙古农业大学,2003.