脂环酸芽孢杆菌（Alicyclobacillus sp.A4）部分糖基水解酶研究及其基因组序列初步分析
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摘要
嗜酸菌是一类广泛分布在酸性环境中极其重要的微生物,然而目前对嗜酸菌的了解还十分有限。脂环酸芽孢杆菌(Alicyclobacillus)作为典型的一个嗜酸耐热菌属,近些年来受到了极大的关注。本文从云南保山高温温泉出水处的土样中分离得到一株嗜酸嗜热的菌株,经16S rDNA比较鉴定,初步确定为脂环酸芽孢杆菌,命名为Alicyclobacillus sp. A4。生长条件实验表明:该株菌的最适生长温度在55–60°C之间,最适生长pH为3.0–3.5。用各种诱导培养基培养后,测到了多种糖基水解酶活性,表明该菌株为产水解酶系丰富的菌株。
在双酶法制糖工艺中,目前广泛应用的是高温淀粉酶和中温糖化酶。然而高温淀粉酶的最适作用温度和pH与中温糖化酶均不相同,需要反复调整pH值和温度。同时,高温淀粉酶不具备降解生淀粉的能力,需要对生淀粉进行糊化,因此造成了生产的较高能耗和环境污染问题。利用可溶性淀粉为碳源,从Alicyclobacillus sp. A4中分离纯化了一个酸性中温α-1,4-淀粉酶(AmyA4)。AmyA4的最适pH值为4.2;最适温度为75°C;在55–75°C范围内都具有很高的活性,这与工业上应用的糖化酶性质相似;同时AmyA4还具有很强的生淀粉降解能力。在模拟双酶法制糖工艺中,以生淀粉为底物,AmyA4在配合商业化糖化酶的作用下,可以达到96.7%的水解率,表明具有很大的应用潜力。
利用大麦葡聚糖为碳源,分离纯化了一个极端酸性β-1,4-葡聚糖酶(CelA4)。CelA4的最适pH为2.6,在酸性pH范围内保持稳定,对大麦β-葡聚糖,纤维素,燕麦木聚糖和甘露聚糖均具有催化活性,同时还能对胃蛋白酶有很高的抗性。在人工模拟胃液中,CelA4可以明显降低大麦豆粕饲料的粘度,具有猪饲料添加剂的应用潜力。通过基质辅助激光解吸离子飞行质谱(MALDI-TOF-MS)和全基因组序列分析,确定了CelA4的基因序列。CelA4编码715个氨基酸,与来源于Alicyclobacillus acidocaldarius第51家族的β-1,4-葡聚糖酶CelB有最高的一致性(44%)。CelA4具有三个明显的结构区,即信号肽区(0–39 AAs)、催化区(40–498 AAs)、和苏氨酸高含量区(499–715 AAs)。对全长的成熟蛋白CelA4F(40–715 AAs)和截短的葡聚糖酶CelA4T(40–498 AAs)分别进行了毕赤酵母的外源表达和性质测定。结果表明,重组的CelA4F和CelA4T具有和原酶CelA4相似的性质。在3.7L发酵罐上,截短的葡聚糖酶CelA4T获得了更高的表达量。SDS-PAGE和脱糖基化后分析表明,重组CelA4F和原酶CelA4一样都发生了截短的现象,苏氨酸高含量区对其表达量有明显作用。
对来源于Alicyclobacillus sp.A4的第九家族β-1,3(4)-葡聚糖酶基因(agl9A)进行了毕赤酵母的外源表达和性质测定。Agl9A与来源于A. acidocaldarius第九家族的葡聚糖酶具有最高的氨基酸一致性(48%)。纯化的重组Agl9A最适pH为5.8,最适温度为75°C,具有钙离子依赖性,对各种中性蛋白酶包括添加到啤酒酿造的蛋白酶Neutrase 0.8L有抗性。在模拟麦芽汁糖化条件下,无论是单独添加Agl9A,还是和木聚糖酶Xyn(商业酶)同时添加均能有效降低麦芽汁的过滤速率和粘度。上述性质表明Agl9A是一个优秀的葡聚糖酶,可应用于啤酒酿造中的葡聚糖降解。
对来源于Alicyclobacillus sp.A4的第十家族β-1,4-木聚糖酶(XynA4)进行了大肠杆菌的外源表达和性质测定。XynA4与来源于Geobacillus stearohermophilus第10家族的木聚糖酶有最高的氨基酸一致性(53%)。纯化的重组XynA4在pH7.0和55°C具有最佳活性,在pH值3.8–9.4范围内,能保持40%以上的最大酶活。该酶还在非常宽的pH范围(pH值2.6–12.0,>80%)内保持稳定。由于XynA4具有良好的热稳定性和pH值稳定性,并且在很宽的pH范围内具有活力,因此它具有在造纸行业中应用的潜力。
鉴于脂环酸芽孢杆菌给饮料行业带来的巨大危害和对极端环境的特殊生长要求,并且其16S rDNA序列与已知全基因序列的A. acidocaldarius DSM 446一致性只有95%。因此对Alicyclobacillus sp. A4的全基因组进行了测序,并对测序结果进行了初步分析。结果表明:该菌与A. acidocaldarius DSM 446相似,有共线性。染色体基因组全长2,815,170 bp,编码2,884个基因,GC含量为46.5%。在染色体基因组编码的2,884个基因中,有2,359个基因(81.8%与A. acidocaldarius DSM 446的序列同源,平均一致性为65.4%。质粒基因组全长80,481 bp,编码88个基因,GC含量为52.1%。在质粒基因组编码的88个基因中,有58个基因(65.9%)与A. acidocaldarius DSM 446质粒pAACI01的序列同源,平均一致性为83.3%。对Alicyclobacillus sp. A4的分泌组蛋白进行了分析,结果表明有10.9%的序列被鉴定为具有明显信号肽序列。另外Alicyclobacillus sp. A4的全基因组共含有31条糖基水解酶序列,其中有6条蛋白序列具有明显的信号肽序列。
Acidophilus, widely distributed in extremely acidic environments, are important microorganisms; the knowledge of this group of microorganisms is very limited however. Alicyclobacillus sp., as the typical thermalacidophilus, has attracted much attention in the recent years. Herein a thermalacidophilus, denoted as A4, was isolated from the soil close to the outflow of a hot spring in Baoshan, Yunnan, China, and was identified as Alicyclobacillus sp. by comparison of the 16s rDNA sequence with known ones. The strain showed optimal growth at 55–60°C and pH 3.0–3.5. Activities of several glycosyl hydrolyses were detected when induced by various carbon sources, suggesting that stain A4 is a producer of multiple glycosyl hydrolyses.
During the double-enzymatic sugar-making process, thermophilicα-amylase and mesophilic saccharifying enzyme are widely applied. Because both enzymes have different optimum temperature and pH, the pH values and temperatures must be adjusted repeatedly. Moreover, thermophilicα-amylase has no ability to degrade raw starch, which must be gelatinized before treatment, thus causing problems such as high energy consumption and environmental contamination. Using soluble starch as carbon source, an acidα-amylase (AmyA4) was isolated and purified. AmyA4 had the optimum pH of 4.2 and optimal temperature at 75°C, showed high activity at 40?65°C, and possessed strong capacity to degrade raw starch. Under simulated double-enzymatic sugar-making conditions, AmyA4 combined with commercialized saccharifying enzyme could catalyze the hydrolysis of 96.7% substrate, and thus has great potential for application.
Using barleyβ-glucan as carbon source, an extremely acidβ-1,4-glucanase (CelA4) was isolated and purified from the culture supernatant of Alicyclobacillus sp. A4. CelA4 showed optimal activity at pH 2.6, was stable in acidic conditions, showed catalytic activity towards various substrates including barleyβ-glucan, cellulose, oat spelt xylan and mannan, and had high resistance to pepsin. Under simulated gastric conditions, CelA4 can effectively reduce the viscosity of soybean-barley meal and has potential to be a candidate of pig feed additives. The gene sequence of CelA4 was determined by the analysis of MALDI-TOF-MS and whole genome sequence. CelA4 coded 715 AAs, and its deduced amino acid sequence shared the highest identity (44%) with an endo-β-1,4-glucansae from Alicyclobacillus acidocaldarius that belongs to family 51 of the glycoside hydrolases. CelA4 has three domains, namely the signal peptide (0–39 AAs), catalytic doamin (40–498 AAs), and threonine-rich region (499-715 AAs). Furthermore, the genes coding for full-length CelA4F (40–715 AAs) and truncated CelA4T (40–498 AAs) were also expressed in Pichia pastries. Biochemical characteristics of CelA4T and CelA4F were similar with native CelA4, but CelA4T achieved higher throughout than CelA4F did in 3.7 L fermentor. Based on the analysis of deglycosylation and SDS-PAGE, CelA4F was determined to be truncated by itself as native CelA4 did, and the threonine-rich region might have a significant effect on enzyme expression. The gene encoding a GH 9 glucanase (Agl9A) from Alicyclobacillus sp. A4 was cloned and expressed in P. pastries. The deduced amino acid sequence of Agl9A shared the highest identity (48%) with an endo-β-1,4-glucansae from A. acidocaldarius that belongs to family 9 of the glycoside hydrolases. The purified recombinant Agl9A had optimal pH at 5.8 and temperature optimumat 65°C, was Ca2+-dependent, and showed strong resistance to various neutral proteases including Neutrase 0.8L (Novozymes), a protease widely added to the mash. Under simulated mashing conditions, addition of Agl9A alone or in combination with a commercial xylanase can both effectively reduce the filtration rate and viscosity. These characteristics indicate that Agl9A is a good candidate to improve glucan degradation in the malting and brewing industry.
The gene coding for a GH 10 xylanase (XynA4) from Alicyclobacillus sp. A4 was cloned and expressed in Escherichia coli. The deduced amino acid sequence of XynA4 shared the highest identity (53%) with an endo-β-1,4-xylanase from Geobacillus stearohermophilus that belongs to family 10 of the glycoside hydrolases. Purified recombinant XynA4 exhibited maximum activity at 55°C and pH 7.0, had broad pH adaptability (>40% activity at pH 3.8?9.4) and stability (retaining >80% activity at pH 2.6?12.0). The superior properties, such as good thermostability and pH stability and adapatability over a broad pH range, make XynA4 promising for application in the paper industry.
Considering the harm to beverage caused by Alicyclobacillus sp., its special demand for extreme environment, and low 16S rDNA sequence identity (only 95%) with A. acidocaldarius DSM 446 (whole genome sequenced), the whole genome sequencing of Alicyclobacillus sp. A4 and elementary analysis was carried out. Results showed that the genome of Alicyclobacillus sp. A4 consisted of a chromosome genome and a plasmid genome, and shared near genomic synteny with A. acidocaldarius DSM 446. The chromosome genome was 2,815,170 bp in length, coded 2,884 genes, and the GC content was 46.5%. Among the 2,884 coding genes, 2,359 (81.8%) genes were identified to be homologous with A. acidocaldarius DSM 446, and the average identity was 65.4%. The plasmid genome length was 80,481 bp, coding 88 genes, with 52.1% GC content. Fifty-eight genes (65.9%) were identified to be the homologues of pAACI01 from A. acidocaldarius DSM 446 with average identity of 83.3%. The secretion of histone proteins was also analyzed, and 10.9% of the sequences were identified with signal peptides. In addition, the genome of Alicyclobacillus sp. A4 contained 31 glycosyl hydrolyse-encoding sequences, and 6 of them had signal peptides.
在双酶法制糖工艺中,目前广泛应用的是高温淀粉酶和中温糖化酶。然而高温淀粉酶的最适作用温度和pH与中温糖化酶均不相同,需要反复调整pH值和温度。同时,高温淀粉酶不具备降解生淀粉的能力,需要对生淀粉进行糊化,因此造成了生产的较高能耗和环境污染问题。利用可溶性淀粉为碳源,从Alicyclobacillus sp. A4中分离纯化了一个酸性中温α-1,4-淀粉酶(AmyA4)。AmyA4的最适pH值为4.2;最适温度为75°C;在55–75°C范围内都具有很高的活性,这与工业上应用的糖化酶性质相似;同时AmyA4还具有很强的生淀粉降解能力。在模拟双酶法制糖工艺中,以生淀粉为底物,AmyA4在配合商业化糖化酶的作用下,可以达到96.7%的水解率,表明具有很大的应用潜力。
利用大麦葡聚糖为碳源,分离纯化了一个极端酸性β-1,4-葡聚糖酶(CelA4)。CelA4的最适pH为2.6,在酸性pH范围内保持稳定,对大麦β-葡聚糖,纤维素,燕麦木聚糖和甘露聚糖均具有催化活性,同时还能对胃蛋白酶有很高的抗性。在人工模拟胃液中,CelA4可以明显降低大麦豆粕饲料的粘度,具有猪饲料添加剂的应用潜力。通过基质辅助激光解吸离子飞行质谱(MALDI-TOF-MS)和全基因组序列分析,确定了CelA4的基因序列。CelA4编码715个氨基酸,与来源于Alicyclobacillus acidocaldarius第51家族的β-1,4-葡聚糖酶CelB有最高的一致性(44%)。CelA4具有三个明显的结构区,即信号肽区(0–39 AAs)、催化区(40–498 AAs)、和苏氨酸高含量区(499–715 AAs)。对全长的成熟蛋白CelA4F(40–715 AAs)和截短的葡聚糖酶CelA4T(40–498 AAs)分别进行了毕赤酵母的外源表达和性质测定。结果表明,重组的CelA4F和CelA4T具有和原酶CelA4相似的性质。在3.7L发酵罐上,截短的葡聚糖酶CelA4T获得了更高的表达量。SDS-PAGE和脱糖基化后分析表明,重组CelA4F和原酶CelA4一样都发生了截短的现象,苏氨酸高含量区对其表达量有明显作用。
对来源于Alicyclobacillus sp.A4的第九家族β-1,3(4)-葡聚糖酶基因(agl9A)进行了毕赤酵母的外源表达和性质测定。Agl9A与来源于A. acidocaldarius第九家族的葡聚糖酶具有最高的氨基酸一致性(48%)。纯化的重组Agl9A最适pH为5.8,最适温度为75°C,具有钙离子依赖性,对各种中性蛋白酶包括添加到啤酒酿造的蛋白酶Neutrase 0.8L有抗性。在模拟麦芽汁糖化条件下,无论是单独添加Agl9A,还是和木聚糖酶Xyn(商业酶)同时添加均能有效降低麦芽汁的过滤速率和粘度。上述性质表明Agl9A是一个优秀的葡聚糖酶,可应用于啤酒酿造中的葡聚糖降解。
对来源于Alicyclobacillus sp.A4的第十家族β-1,4-木聚糖酶(XynA4)进行了大肠杆菌的外源表达和性质测定。XynA4与来源于Geobacillus stearohermophilus第10家族的木聚糖酶有最高的氨基酸一致性(53%)。纯化的重组XynA4在pH7.0和55°C具有最佳活性,在pH值3.8–9.4范围内,能保持40%以上的最大酶活。该酶还在非常宽的pH范围(pH值2.6–12.0,>80%)内保持稳定。由于XynA4具有良好的热稳定性和pH值稳定性,并且在很宽的pH范围内具有活力,因此它具有在造纸行业中应用的潜力。
鉴于脂环酸芽孢杆菌给饮料行业带来的巨大危害和对极端环境的特殊生长要求,并且其16S rDNA序列与已知全基因序列的A. acidocaldarius DSM 446一致性只有95%。因此对Alicyclobacillus sp. A4的全基因组进行了测序,并对测序结果进行了初步分析。结果表明:该菌与A. acidocaldarius DSM 446相似,有共线性。染色体基因组全长2,815,170 bp,编码2,884个基因,GC含量为46.5%。在染色体基因组编码的2,884个基因中,有2,359个基因(81.8%与A. acidocaldarius DSM 446的序列同源,平均一致性为65.4%。质粒基因组全长80,481 bp,编码88个基因,GC含量为52.1%。在质粒基因组编码的88个基因中,有58个基因(65.9%)与A. acidocaldarius DSM 446质粒pAACI01的序列同源,平均一致性为83.3%。对Alicyclobacillus sp. A4的分泌组蛋白进行了分析,结果表明有10.9%的序列被鉴定为具有明显信号肽序列。另外Alicyclobacillus sp. A4的全基因组共含有31条糖基水解酶序列,其中有6条蛋白序列具有明显的信号肽序列。
Acidophilus, widely distributed in extremely acidic environments, are important microorganisms; the knowledge of this group of microorganisms is very limited however. Alicyclobacillus sp., as the typical thermalacidophilus, has attracted much attention in the recent years. Herein a thermalacidophilus, denoted as A4, was isolated from the soil close to the outflow of a hot spring in Baoshan, Yunnan, China, and was identified as Alicyclobacillus sp. by comparison of the 16s rDNA sequence with known ones. The strain showed optimal growth at 55–60°C and pH 3.0–3.5. Activities of several glycosyl hydrolyses were detected when induced by various carbon sources, suggesting that stain A4 is a producer of multiple glycosyl hydrolyses.
During the double-enzymatic sugar-making process, thermophilicα-amylase and mesophilic saccharifying enzyme are widely applied. Because both enzymes have different optimum temperature and pH, the pH values and temperatures must be adjusted repeatedly. Moreover, thermophilicα-amylase has no ability to degrade raw starch, which must be gelatinized before treatment, thus causing problems such as high energy consumption and environmental contamination. Using soluble starch as carbon source, an acidα-amylase (AmyA4) was isolated and purified. AmyA4 had the optimum pH of 4.2 and optimal temperature at 75°C, showed high activity at 40?65°C, and possessed strong capacity to degrade raw starch. Under simulated double-enzymatic sugar-making conditions, AmyA4 combined with commercialized saccharifying enzyme could catalyze the hydrolysis of 96.7% substrate, and thus has great potential for application.
Using barleyβ-glucan as carbon source, an extremely acidβ-1,4-glucanase (CelA4) was isolated and purified from the culture supernatant of Alicyclobacillus sp. A4. CelA4 showed optimal activity at pH 2.6, was stable in acidic conditions, showed catalytic activity towards various substrates including barleyβ-glucan, cellulose, oat spelt xylan and mannan, and had high resistance to pepsin. Under simulated gastric conditions, CelA4 can effectively reduce the viscosity of soybean-barley meal and has potential to be a candidate of pig feed additives. The gene sequence of CelA4 was determined by the analysis of MALDI-TOF-MS and whole genome sequence. CelA4 coded 715 AAs, and its deduced amino acid sequence shared the highest identity (44%) with an endo-β-1,4-glucansae from Alicyclobacillus acidocaldarius that belongs to family 51 of the glycoside hydrolases. CelA4 has three domains, namely the signal peptide (0–39 AAs), catalytic doamin (40–498 AAs), and threonine-rich region (499-715 AAs). Furthermore, the genes coding for full-length CelA4F (40–715 AAs) and truncated CelA4T (40–498 AAs) were also expressed in Pichia pastries. Biochemical characteristics of CelA4T and CelA4F were similar with native CelA4, but CelA4T achieved higher throughout than CelA4F did in 3.7 L fermentor. Based on the analysis of deglycosylation and SDS-PAGE, CelA4F was determined to be truncated by itself as native CelA4 did, and the threonine-rich region might have a significant effect on enzyme expression. The gene encoding a GH 9 glucanase (Agl9A) from Alicyclobacillus sp. A4 was cloned and expressed in P. pastries. The deduced amino acid sequence of Agl9A shared the highest identity (48%) with an endo-β-1,4-glucansae from A. acidocaldarius that belongs to family 9 of the glycoside hydrolases. The purified recombinant Agl9A had optimal pH at 5.8 and temperature optimumat 65°C, was Ca2+-dependent, and showed strong resistance to various neutral proteases including Neutrase 0.8L (Novozymes), a protease widely added to the mash. Under simulated mashing conditions, addition of Agl9A alone or in combination with a commercial xylanase can both effectively reduce the filtration rate and viscosity. These characteristics indicate that Agl9A is a good candidate to improve glucan degradation in the malting and brewing industry.
The gene coding for a GH 10 xylanase (XynA4) from Alicyclobacillus sp. A4 was cloned and expressed in Escherichia coli. The deduced amino acid sequence of XynA4 shared the highest identity (53%) with an endo-β-1,4-xylanase from Geobacillus stearohermophilus that belongs to family 10 of the glycoside hydrolases. Purified recombinant XynA4 exhibited maximum activity at 55°C and pH 7.0, had broad pH adaptability (>40% activity at pH 3.8?9.4) and stability (retaining >80% activity at pH 2.6?12.0). The superior properties, such as good thermostability and pH stability and adapatability over a broad pH range, make XynA4 promising for application in the paper industry.
Considering the harm to beverage caused by Alicyclobacillus sp., its special demand for extreme environment, and low 16S rDNA sequence identity (only 95%) with A. acidocaldarius DSM 446 (whole genome sequenced), the whole genome sequencing of Alicyclobacillus sp. A4 and elementary analysis was carried out. Results showed that the genome of Alicyclobacillus sp. A4 consisted of a chromosome genome and a plasmid genome, and shared near genomic synteny with A. acidocaldarius DSM 446. The chromosome genome was 2,815,170 bp in length, coded 2,884 genes, and the GC content was 46.5%. Among the 2,884 coding genes, 2,359 (81.8%) genes were identified to be homologous with A. acidocaldarius DSM 446, and the average identity was 65.4%. The plasmid genome length was 80,481 bp, coding 88 genes, with 52.1% GC content. Fifty-eight genes (65.9%) were identified to be the homologues of pAACI01 from A. acidocaldarius DSM 446 with average identity of 83.3%. The secretion of histone proteins was also analyzed, and 10.9% of the sequences were identified with signal peptides. In addition, the genome of Alicyclobacillus sp. A4 contained 31 glycosyl hydrolyse-encoding sequences, and 6 of them had signal peptides.
引文
(1) Akiba, S, Yamamoto, K, Kumagai, H. Effects of size of carbohydrate chain on protease digestion of Aspergillus niger endo-beta-1,4-glucanase. Bioscience, biotechnology, and biochemistry 1995, 59: 1048
(2) Albuquerque L, Rainey FA, Chung AP, Sunna A, Nobre MF, Grote R, Antranikian G. Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of Sao Miguel in the Azores. Int J Syst Evol Microbiol 2000, 50: 451-457
(3) Almirall M, Esteve-Garcia E. In vitro stability of aβ-glucanase preparation from Trichoderma longibrachiatum and its effect in a barley based diet fed to broiler chicks.Время, 2009,54: 149-158
(4) An H, Peavy T, Hedrick J, Lebrilla C. Determination of N-glycosylation sites and site heterogeneity in glycoproteins. Anal Chem 2003, 75: 5628-5637
(5) Aono R, Hammura M, Yamamoto M, Asano T. Isolation of extracellular 28- and 42-kilodalton beta-1,3-glucanases and comparison of three beta-1,3-glucanases produced by Bacillus circulans IAM1165. Appl Environ Microbiol 1995, 61: 122-129
(6) Aquino AC, Jorge JA, Terenzi HF, Polizeli ML. Studies on a thermostable alpha-amylase from the thermophilic fungus Scytalidium thermophilum. Appl Microbiol Biotechnol 2003, 61: 323-328
(7) Bamforth CW. The adaptability, purification and properties of exo-beta-1,3-glucanase from the fungus Trichoderma reesei. Biochem J 1980, 191: 863-866
(8) Belev TN, Singh M, McCarthy JE. A fully modular vector system for the optimization of gene expression in Escherichia coli. Plasmid 1991, 26: 147-150
(9) Bevilacqua A, Sinigaglia M, Corbo MR. Alicyclobacillus acidoterrestris: new methods for inhibiting spore germination. Int J Food Microbiol 2008, 125: 103-110
(10) Borlinghaus A, Engel R. Alicyclobacillus incidence in commercial apple juice concentrate (AJC) supplies-method development and validation. Fruit processing 1997, 7: 262-266
(11) Buliga GS, Brant DA, Fincher GB. The sequence statistics and solution conformation of a barley (1-3, 1-4)-beta-D-glucan. Carbohydr Res 1986, 157: 139-156
(12) Burnell T, Cromwell G, Stahly T. Effects of dried whey and copper sulfate on the growth responses to organic acid in diets for weanling pigs. Journal of animal science 1988, 66: 1100
(13) Campbell LL. Purification and properties of an alpha-amylase from facultative thermophilic bacteria. Arch Biochem 1955, 54: 154-161
(14) Cebe R, Geiser M. Rapid and easy thermodynamic optimization of the 5'-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli. Protein Expr Purif 2006, 45: 374-380
(15) Celestino, K., Cunha, R., Felix, C., 2006. Characterization of aβ-glucanase produced byRhizopus microsporus var. microsporus, and its potential for application in the brewing industry. BMC biochemistry, 7, 23
(16) Celik E, Calik P, Oliver SG. Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast 2009, 26: 473-484
(17) Chadha BS, Rubinder K, Saini HS. Constitutive alpha-amylase producing mutant and recombinant haploid strains of thermophilic fungus Thermomyces lanuginosus. Folia Microbiol (Praha) 2005, 50: 133-140
(18) Chae HJ, Delisa MP, Cha HJ, Weigand WA, Rao G, Bentley WE. Framework for online optimization of recombinant protein expression in high-cell-density Escherichia coli cultures using GFP-fusion monitoring. Biotechnol Bioeng 2000, 69: 275-285
(19) Chang GD, Li ZL, Qin JY, Ma CQ, Luo YZ, Xu P. Optimization of fermentation of recombinant human Endostatin (rh-Endostatin) expression in Escherichia coli. Sheng Wu Gong Cheng Xue Bao 2005, 21: 662-666
(20) Chang SS, Kang DH. Alicyclobacillus spp. in the fruit juice industry: history, characteristics, and current isolation/detection procedures. Crit Rev Microbiol 2004, 30: 55-74
(21) Chang SW, Lee GC, Shaw JF. Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase. J Agric Food Chem 2006, 54: 815-822
(22) Clark S, Muslin E, Henson C. Effect of adding and removing N-glycosylation recognition sites on the thermostability of barley {alpha}-glucosidase. Protein Engineering Design and Selection 2004, 17: 245
(23) Connor CJ, Luo H, McSpadden Gardener BB, Wang HH. Development of a real-time PCR-based system targeting the 16S rRNA gene sequence for rapid detection of Alicyclobacillus spp. in juice products. International Journal of Food Microbiology 2005, 99: 229-235
(24) Cotelle N, Lohez M, Cotelle P, Hénichart J. Conformational study of the threonine-rich C-terminal pentapeptide of peptide T. Biochemical and biophysical research communications 1990, 171: 596-602
(25) Dai X. Yeast Pichia pastoris-a notable heterologous gene expression system. Wei Sheng Wu Xue Bao 1997, 37: 483-485
(26) Danicke S, Vahjen W, Simon O, Jeroch H. Effects of dietary fat type and xylanase supplementation to rye-based broiler diets on selected bacterial groups adhering to the intestinal epithelium on transit time of feed, and on nutrient digestibility. Poult Sci 1999, 78: 1292-1299
(27) Darland G, Brock T. Bacillus acidocaldarius sp. nov., an acidophilic thermophilic spore-forming bacterium. Microbiology 1971, 67: 9
(28) de la Cruz J, Pintor-Toro JA, Benitez T, Llobell A. Purification and characterization of an endo-beta-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism. J Bacteriol 1995, 177: 1864-1871
(29) Deinhard G, Blanz P, Poralla K, Altan E. Bacillus acidoterrestris sp. nov., a new thermotolerantacidophile isolated from different soils. Systematic and Applied Microbiology 1987, 10: 47-53
(30) Delcher A, Harmon D, Kasif S. Improved microbial gene identification with GLIMMER. Nucleic acids research 1999, 27: 4636
(31) Dersjant-Li Y, Schulze H, Schrama JW, Verreth JA, Verstegen MW. Feed intake, growth, digestibility of dry matter and nitrogen in young pigs as affected by dietary cation-anion difference and supplementation of xylanase. J Anim Physiol Anim Nutr (Berl) 2001, 85: 101-109
(32) Di Carlo FJ, Redfern S. alpha-Amylase from Bacillus subtilis; effects of inhibitors upon liquefaction and dextrinization. Arch Biochem 1948, 17: 1
(33) Dybkjaer L, Jacobsen A, Togersen F, et al. Eating and drinking activity of newly weaned piglets: Effects of individual characteristics, social mixing, and addition of extra zinc to the feed. Journal of animal science 2006, 84: 702
(34) Eckert K, Ernst HA, Schneider E, Larsen S, Lo Leggio L. Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA. Acta Crystallogr D Biol Crystallogr 2003, 59: 139-141
(35) Eckert K, Schneider E. A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases. Eur J Biochem 2003, 270: 3593-3602
(36) Eckert K, Zielinski F, Lo Leggio L, Schneider, E. Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009. Appl Microbiol Biotechnol 2002, 60: 428-436
(37) Eckert K, Schneider E. A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases. Eur J Biochem 2003, 270: 3593-602
(38) Erasmus JA. Some features of coagulase positive staphylococci from bovine milk. 1. Carbohydrate metabolism: comparison of conventional techniques and the API 50 CH system. Onderstepoort J Vet Res 1985, 52: 25-29
(39) Fidler AE, Lin JS, Lun S, Ng Chie W, Western A, Stent V, McNatty KP. Production of biologically active tethered ovine FSHbetaalpha by the methylotrophic yeast Pichia pastoris. J Mol Endocrinol 2003, 30: 213-225
(40) Gabert V, Sauer W, Li S, Fan M. Exocrine pancreatic secretions in young pigs fed diets containing faba beans (Vicia faba) and peas (Pisum sativum): concentrations and flows of total, protein-bound and free amino acids. Journal of the Science of Food and Agriculture 1999, 70: 256-262
(41) Gangadharan D, Nampoothiri KM, Sivaramakrishnan S, Pandey A. Biochemical characterization of raw-starch-digesting alpha amylase purified from Bacillus amyloliquefaciens. Appl Biochem Biotechnol 2009, 158: 653-662
(42) Garrett J, Kretz K, O'Donoghue E, Kerovuo J, Kim W, Barton N, Hazlewood G, Short J, Robertson D, Gray K. Enhancing the thermal tolerance and gastric performance of a microbialphytase for use as a phosphate-mobilizing monogastric-feed supplement. Applied and Environmental Microbiology 2004, 70: 3041-3046
(43) Gessesse A. Purification and properties of two thermostable alkaline xylanases from an alkaliphilic bacillus sp. Appl Environ Microbiol 1998, 64: 3533-3535
(44) Gianinetti A. A theoretical framework for beta-glucan degradation during barley malting. Theory Biosci 2009, 128: 97-108.
(45) Goto K, Matsubara H, Mochida K, Matsumura T, Hara Y, Niwa M, Yamasato K. Alicyclobacillus herbarius sp. nov., a novel bacterium containing omega-cycloheptane fatty acids, isolated from herbal tea. Int J Syst Evol Microbiol 2002, 52: 109-113
(46) Graham H, Fadel JG, Newman CW, Newman RK. Effect of pelleting and beta-glucanase supplementation on the ileal and fecal digestibility of a barley-based diet in the pig. J Anim Sci 1989, 67: 1293-1298
(47) Groenewald WH, Gouws PA, Witthuhn RC. Isolation and identification of species of Alicyclobacillus from orchard soil in the Western Cape, South Africa. Extremophiles 2008, 12: 159-163
(48) Groenewald WH, Gouws PA, Witthuhn RC. Isolation, identification and typification of Alicyclobacillus acidoterrestris and Alicyclobacillus acidocaldarius strains from orchard soil and the fruit processing environment in South Africa. Food Microbiol 2009, 26: 71-76
(49) Guo X, You XY, Liu LJ, Zhang JY, Liu SJ, Jiang CY. Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine. Int J Syst Evol Microbiol 2009, 59: 2415-2420
(50) Haraldsson A, Veide J, Andlid T, Alminger M, Sandberg A. Degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. Journal of agricultural and food chemistry 2005, 53: 5438-5444
(51) Hasegawa S, Nordin JH. Enzymes that hydrolyze fungal cell wall polysaccharides. I. Purification and properties of an endo-alpha-D-(1,3)-glucanase from Trichoderma. J Biol Chem 1969, 244: 5460-5470
(52) Hayashida S, Teramoto Y, Inoue T. Production and Characteristics of Raw-Potato-Starch-Digesting alpha-Amylase from Bacillus subtilis 65. Appl Environ Microbiol 1988, 54: 1516-1522
(53) Hayashida S, Teramoto Y. Production and Characteristics of Raw-Starch-Digesting alpha-Amylase from a Protease-Negative Aspergillus ficum Mutant. Appl Environ Microbiol 1986, 52: 1068-1073
(54) Hien NH, Fleet GH. Separation and characterization of six (1 leads to 3)-beta-glucanases from Saccharomyces cerevisiae. J Bacteriol 1983, 156: 1204-1213
(55) Hirsch K, Simon O, Vahjen W. Influence of a xylanase feed additive on Lactobacillus species in the jejunum of piglets. Berl Munch Tierarztl Wochenschr 2006, 119: 486-492
(56) Hu S, Li L, Qiao J, Guo Y, Cheng L, Liu J. Codon optimization, expression, and characterizationof an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris. Protein Expr Purif 2006, 47: 249-257
(57) Hwang KY, Song HK, Chang C, Lee J, Lee SY, Kim KK, Choe S. Crystal structure of thermostable alpha-amylase from Bacillus licheniformis refined at 1.7 A resolution. Mol Cells 1997, 7: 251-258
(58) Inagaki K, Nakahira K, Mukai K, Tamura T, Tanaka H. Gene cloning and characterization of an acidic xylanase from Acidobacterium capsulatum. Biosci Biotechnol Biochem 1998, 62: 1061?1067.
(59) Inborr J, Puhakka J, Bakker JG, Van der Meulen J. Beta-glucanase and xylanase activities in stomach and ileum of growing pigs fed wheat bran based diets with and without enzyme treatment. Arch Tierernahr 1999, 52: 263-274
(60) Jeang CL, Chen LS, Chen MY, Shiau RJ. Cloning of a gene encoding raw-starch-digesting amylase from a Cytophaga sp. and its expression in Escherichia coli. Appl Environ Microbiol 2002, 68: 3651-3654
(61) Jeang CL, Lee YH, Chang LW. Purification and characterization of a raw-starch digesting amylase from a soil bacterium-Cytophaga sp. Biochem Mol Biol Int 1995, 35: 549-557
(62) Jiang ZB, Song HT, Ma LX. Secreted expression of Bacillus pumilus xylanase gene in Pichia pastoris and study on enzymatic properties. Sheng Wu Gong Cheng Xue Bao 2003, 19: 50-55
(63) Joao H, Scragg I, Dwek R. Effects of glycosylation on protein conformation and amide proton exchange rates in RNase B. FEBS Letters 1992, 307: 343-346
(64) Jonsson LJ, Saloheimo M, Penttila M. Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr Genet 1997, 32: 425-430
(65) Joseleau J, Comtat J, Ruel K. Chemical structure of xylans and their interaction in the plant cell walls. Progress in Biotechnology 1992, 7: 1-5
(66) Jungo C, Marison I, von Stockar U. Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures. J Biotechnol 2007, 128: 824-837
(67) Kanehisa M, Araki M, Goto S. KEGG for linking genomes to life and the environment. Nucleic acids research 2008, 36: D480
(68) Karlin S, Bucher P, Brendel V, et al. Statistical methods and insights for protein and DNA sequences. Annual review of biophysics and biophysical chemistry 1991, 20: 175-203
(69) Khatri NK, Hoffmann F. Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol Bioeng 2006, 93: 871-879
(70) Kim CH, Sata H, Taniguchi H, Maruyama Y. Cloning and expression of raw-starch-digesting alpha-amylase gene from Bacillus circulans F-2 in Escherichia coli. Biochim Biophys Acta 1990, 1048: 223-230
(71) Kim J, Nanmori T, Shinke R. Thermostable, Raw-Starch-Digesting Amylase from Bacillus stearothermophilus. Appl Environ Microbiol 1989, 55: 1638-1639
(72) Kim KH, Kim YO, Ko BS, Youn HJ, Lee DS. Over-expression of the gene (bglBC1) from Bacillus circulans encoding an endo-beta-1,3-1,4-glucanase useful for the preparation of oligosaccharides from barley beta-glucan. Biotechnol Lett 2004, 26: 1749-1755
(73) Kotake T, Nakagawa N, Takeda K, Sakurai N. Purification and characterization of wall-bound exo-1,3-beta-D-glucanase from barley (Hordeum vulgare L) seedlings. Plant Cell Physiol 1997, 38: 194-200
(74) Kubicek CP. beta-Glucosidase excretion by Trichoderma pseudokoningii: correlation with cell wall bound beta-1.3-glucanase activities. Arch Microbiol 1982, 132: 349-354
(75) Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680-685
(76) Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 1985, 82: 6955-6959
(77) Lee CC, Kibblewhite-Accinelli RE, Wagschal K, Robertson GH, Wong DW. Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library. Extremophiles 2006, 10: 295-300
(78) Lee CK, Darah I, Ibrahim CO. Enzymatic deinking of laser printed office waste papers: some governing parameters on deinking efficiency. Bioresour Technol 2007, 98: 1684-1689
(79) Lee S, Oneda H, Minoda M, Tanaka A, Inouye K. Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis alpha-amylases and insights into engineering alpha-amylase variants active under acidic conditions. J Biochem 2006, 139: 997-1005
(80) Lee TH, Lim PO, Lee YE. Cloning, characterization, and expression of xylanase A gene from Paenibacillus sp. DG-22 in Escherichia coli. J Microbiol Biotechnol 2007, 17: 29-36
(81) Li N, Shi P, Yang P, Wang Y, Luo H, Bai Y, Zhou Z, et al. A xylanase with high pH stability from Streptomyces sp. S27 and its carbohydrate-binding module with/without linker-region-truncated versions. Appl Microbiol Biotechnol 2009, 83:99-107
(82) Li S, Sauer WC, Huang SX, Gabert VM. Effect of beta-glucanase supplementation to hulless barley- or wheat-soybean meal diets on the digestibilities of energy, protein, beta-glucans, and amino acids in young pigs. J Anim Sci 1996, 74: 1649-1656
(83) Li Y, Lu J, Gu G, Shi Z, Mao Z. Mathematical modeling for prediction of endo-xylanase activity and arabinoxylans concentration during mashing of barley malts for brewing. Biotechnol Lett 2004, 26: 779-785
(84) Lim HK, Choi SJ, Kim KY, Jung KH. Dissolved-oxygen-stat controlling two variables for methanol induction of rGuamerin in Pichia pastoris and its application to repeated fed-batch. Appl Microbiol Biotechnol 2003, 62: 342-348
(85) Liu R, Lin Q, Sun Y, Qiu Y, Xu T, Ding H, Guo X. Optimization of fermentation conditions for maximal recombinant hepatitis B surface antigen particle production in Pichia pastoris. Sheng Wu Gong Cheng Xue Bao 2008, 24: 2098-2105
(86) Liu XD, Xu Y. A novel raw starch digesting alpha-amylase from a newly isolated Bacillus sp. YX-1: purification and characterization. Bioresour Technol 2008, 99: 4315-4320
(87) Liu YG, Mitsukawa N, Oosumi T, Whittier RF. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 1995, 8: 457-463
(88) Loginova LG, Karpukhina S. Some features of alpha-amylase in thermophilic bacteria. Dokl Akad Nauk SSSR 1968, 180: 225-228
(89) Luo H, Yang J, Yang P, et al. Gene cloning and expression of a new acidic family 7 endo-beta-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1. Appl Microbiol Biotechnol, 85: 1015-1023
(90) Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22: 249-270
(91) Machius M, Wiegand G, Huber R. Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution. J Mol Biol 1995, 246: 545-559
(92) Manimaran A, Kumar KS, Permaul K, Singh S. Hyper production of cellulase-free xylanase by Thermomyces lanuginosus SSBP on bagasse pulp and its application in biobleaching. Appl Microbiol Biotechnol 2009, 81: 887-893
(93) Marques S, Pala H, Alves L, Amaral-Collaco MT, Gama FM, Girio FM. Characterisation and application of glycanases secreted by Aspergillus terreus CCMI 498 and Trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper. J Biotechnol 2003, 100: 209-219
(94) Marshall JJ. Degradation of barley glucan by a purified (1 yields 4)-beta-D-glucanase from the snail, Helix pomatia. Carbohydr Res 1975, 42: 203-207
(95) Martin KL, McDougall BM, Unkles SE, Seviour RJ. The three beta-1,3-glucanases from Acremonium blochii strain C59 appear to be encoded by separate genes. Mycol Res 2006, 110: 66-74
(96) Mathlouthi N, Saulnier L, Quemener B, Larbier M. Xylanase, beta-glucanase, and other side enzymatic activities have greater effects on the viscosity of several feedstuffs than xylanase and beta-glucanase used alone or in combination. J Agric Food Chem 2002, 50: 5121-5127
(97) Matsubara H, Goto K, Matsumura T, Mochida K, Iwaki M, Niwa M, Yamasato K. Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages. Int J Syst Evol Microbiol 2002, 52: 1681-1685
(98) Mattanovich D, Weik R, Thim S, Kramer W, Bayer K, Katinger H. Optimization of recombinant gene expression in Escherichia coli. Ann N Y Acad Sci 1996, 782: 182-190
(99) Matzke J, Schwermann B, Bakker EP. Acidostable and acidophilic proteins: the example of the alpha-amylase from Alicyclobacillus acidocaldarius. Comp Biochem Physiol A Physiol 1997, 118: 475-479
(100) McCarthy T, Hanniffy O, Savage AV, Tuohy MG. Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3-1,4-linkedglucans. Int J Biol Macromol 2003, 33: 141-148
(101) McQueen R, Van Soest P. Fungal cellulase and hemicellulase prediction of forage digestibility. Journal of Dairy Science 1975, 58: 1482
(102) Mikami B, Adachi M, Kage T, Sarikaya E, Nanmori T, Shinke R, Utsumi S. Structure of raw starch-digesting Bacillus cereus beta-amylase complexed with maltose. Biochemistry 1999, 38: 7050-7061
(103) Minekus M, Marteau P, Havenaar R, HUIS I. A multicompartmental dynamic computer-controlled model simulating the stomach and small intestine. ATLA Alternatives to laboratory animals 1995, 23: 197-209
(104) Monfort A, Blasco A, Prieto JA, Sanz P. Combined Expression of Aspergillus nidulans Endoxylanase X24 and Aspergillus oryzae (alpha)-Amylase in Industrial Baker's Yeasts and Their Use in Bread Making. Appl Environ Microbiol 1996, 62: 3712-3715
(105) Monma M, Kainuma K. Heterogeneity of the glucoamylase components of the raw-starch-digesting amylase from Chalara paradoxa. Carbohydr Res 1992, 227: 385-388
(106) Morana A, Esposito A, Maurelli L, Ruggiero G, Ionata E, Rossi M, La Cara F.A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius. Protein Pept Lett 2008, 15: 1017-1021
(107) Mountfort DO, Asher RA. Production of alpha-Amylase by the Ruminal Anaerobic Fungus Neocallimastix frontalis. Appl Environ Microbiol 1988, 54: 2293-2299
(108) Nakamura T, Namba H, Ohmoto T, Liu Y, Hirata D, Miyakawa T. Cloning and characterization of the Saccharomyces cerevisiae SVS1 gene which encodes a serine-and threonine-rich protein required for vanadate resistance. Gene 1995, 165: 25-29
(109) Niwa M, Kuriyama A. A. acidoterrestris rapid detection kit. Fruit processing 2003: 328-331
(110) Ohya T, Ohyama M, Kobayashi K. Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation. Biotechnol Bioeng 2005, 90: 876-887
(111) Oka C, Tanaka M, Muraki M, Harata K, Suzuki K, Jigami Y. Human lysozyme secretion increased by alpha-factor pro-sequence in Pichia pastoris. Biosci Biotechnol Biochem 1999, 63: 1977-1983
(112) Oliveira R, Clemente JJ, Cunha AE, Carrondo MJ. Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation. J Biotechnol 2005, 116: 35-50
(113) Pala H, Mota M, Gama FM. Enzymatic versus chemical deinking of non-impact ink printed paper. J Biotechnol 2004, 108: 79-89
(114) Yang P, Shi P, Wang Y, Bai Y, Meng K, Luo H, Yuan T, Yao B. Cloning and Overexpression of a Paenibacillusβ-Glucanase in Pichia pastoris: Purification and Characterization of the Recombinant Enzyme. Journal of microbiology and biotechnology 2007, 17: 58-66
(115) Penayanez A, Vilches A, Moralara RJ. Application of Somogyi's Method to the Determination ofAmylase in the Urine. Rev Clin Esp 1963, 91: 93-96
(116) Plantz BA, Sinha J, Villarete L, Nickerson KW, Schlegel VL. Pichia pastoris fermentation optimization: energy state and testing a growth-associated model. Appl Microbiol Biotechnol 2006, 72: 297-305
(117) Ponte PI, Lordelo MM, Guerreiro CI, Soares MC, Mourao JL, Crespo JP, Crespo DG, et al. Crop beta-glucanase activity limits the effectiveness of a recombinant cellulase used to supplement a barley-based feed for free-range broilers. Br Poult Sci 2008, 49: 347-359
(118) Rajendran S, Radha C, Prakash V. Mechanism of solvent-induced thermal stabilization of alpha-amylase from Bacillus amyloliquefaciens. Int J Pept Protein Res 1995, 45: 122-128
(119) Ramchuran SO, Holst O, Karlsson EN. Effect of postinduction nutrient feed composition and use of lactose as inducer during production of thermostable xylanase in Escherichia coli glucose-limited fed-batch cultivations. J Biosci Bioeng 2005, 99: 477-484
(120) Ramon R, Feliu JX, Cos O, Montesinos JL, Berthet FX, Valero F. Improving the monitoring of methanol concentration during high cell density fermentation of Pichia pastoris. Biotechnol Lett 2004, 26: 1447-1452
(121) Rapp P. Formation, separation and characterization of three beta-1,3-glucanases from Sclerotium glucanicum. Biochim Biophys Acta 1992, 1117: 7-14
(122) Rauceo J, De Armond R, Otoo H, Kahn P, Klotz S, Gaur N, Lipke P. Threonine-rich repeats increase fibronectin binding in the Candida albicans adhesin Als5p. Eukaryotic Cell 2006, 5: 1664
(123) Richardson TH, Tan X, Frey G, Callen W, Cabell M, Lam D, Macomber J, et al. A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable alpha-amylase. J Biol Chem 2002, 277: 26501-26507
(124) Saha BC, Lecureux LW, Zeikus JG. Raw starch adsorption-desorption purification of a thermostable beta-amylase from Clostridium thermosulfurogenes. Anal Biochem 1988, 175: 569-572
(125) Saito N. alpha-Amylase inhibitor from fungus Cladosporium herbarum F-828. J Biol Chem 1982, 257: 3120-3125
(126) Schwermann B, Pfau K, Liliensiek B, Schleyer M, Fischer T, Bakker EP. Purification, properties and structural aspects of a thermoacidophilic alpha-amylase from Alicyclobacillus acidocaldarius atcc 27009. Insight into acidostability of proteins. Eur J Biochem 1994, 226: 981-991
(127) Shaw A, Bott R, Day A. Protein engineering ofα-amylase for low pH performance. Current opinion in biotechnology 1999, 10: 349-352
(128) Simbahan J, Drijber R, Blum P. Alicyclobacillus vulcanalis sp. nov., a thermophilic, acidophilic bacterium isolated from Coso Hot Springs, California, USA. Int J Syst Evol Microbiol 2004, 54: 1703-1707
(129) Simpson HD, Haufler UR, Daniel RM. An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga. Biochem J 1991, 277: 413-417
(130) Singh VK, Mangalam AK, Dwivedi S, Naik S. Primer premier: program for design of degenerate primers from a protein sequence. Biotechniques 1998, 24: 318-319
(131) Spllttstoesser D, Churey J, Lee C. Growth characteristics of aciduric sporeforming bacilli isolated from fruit juices. Journal of Food Protectionand 1994, 174: 1080-1083
(132) Suzek B, Ermolaeva M, Schreiber M. A probabilistic method for identifying start codons in bacterial genomes. Bioinformatics 2001, 17: 1123
(133) Tabernero C, Sánchez-Torres J, Pérez P, Santamaría RI Cloning and DNA sequencing of xyaA, a gene encoding an endo-beta-1,4-xylanase from an alkalophilic Bacillus strain (N137). Appl Environ Microbiol 1995, 61:2420?2424.
(134) Tager M, Kroning H, Thiel U, Ansorge S. Membrane-bound proteindisulfide isomerase (PDI) is involved in regulation of surface expression of thiols and drug sensitivity of B-CLL cells. Exp Hematol 1997, 25: 601-607
(135) Tan LU, Mayers P, Illing M, Saddler JN. The copurification of beta-glucosidase, beta-xylosidase, and 1,3-beta-glucanase in two separate enzyme complexes isolated from Trichoderma harzianum E58. Biochem Cell Biol 1987, 65: 822-832
(136) Tanaka A, Hoshino E. Similarities between the thermal inactivation kinetics of Bacillus amyloliquefaciens alpha-amylase in an aqueous solution of sodium dodecyl sulphate and the kinetics in the solution of anionic-phospholipid vesicles. Biotechnol Appl Biochem 2003, 38: 175-181
(137) Tangarone B, Royer JC, Nakas JP. Purification and Characterization of an Endo-(1,3)-beta-D-Glucanase from Trichoderma longibrachiatum. Appl Environ Microbiol 1989, 55: 177-184
(138) Telenius H, Carter NP, Bebb CE, Nordenskjold M, Ponder BA, Tunnacliffe A. Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 1992, 13: 718-725
(139) Teng D, Fan Y, Yang YL, Tian ZG, Luo J, Wang JH. Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Appl Microbiol Biotechnol 2007, 74: 1074-1083
(140) Thornton CR. Use of monoclonal antibodies to quantify the dynamics of alpha-galactosidase and endo-1,4-beta-glucanase production by Trichoderma hamatum during saprotrophic growth and sporulation in peat. Environ Microbiol 2005, 7: 737-749
(141) Tricarico JM, Dawson KA. Influence of supplemental endoglucanase or xylanase on volatile fatty acid production from ruminant feed by ruminal in vitro cultures. Arch Anim Nutr 2005, 59: 325-334
(142) Tsuruoka N, Isono Y, Shida O, Hemmi H, Nakayama T, Nishino T. Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan. Int J Syst Evol Microbiol 2003, 53: 1081-1084
(143) Violet M, Meunier JC. Kinetic study of the irreversible thermal denaturation of Bacilluslicheniformis alpha-amylase. Biochem J 1989, 263: 665-670
(144) Von Wettstein D, Warner J, Kannangara CG. Supplements of transgenic malt or grain containing (1,3-1,4)-beta-glucanase increase the nutritive value of barley-based broiler diets to that of maize. Br Poult Sci 2003, 44: 438-449
(145) Walker M, Phillips CA. The effect of preservatives on Alicyclobacillus acidoterrestris and Propionibacterium cyclohexanicum in fruit juice. Food Control 2008, 19: 974-981
(146) Walls I, Chuyate R. Spoilage of fruit juices by Alicyclobacillus acidoterrestris: Alicyclobacillus in the food industry. Food Australia 2000, 52: 286-288
(147) Walsh GA, Murphy RA, Killeen GF, Headon DR, Power RF. Technical note: detection and quantification of supplemental fungal beta-glucanase activity in animal feed. J Anim Sci 1995, 73: 1074-1076
(148) Walther R, Atlas E, Carrigan A, et al. A serine/threonine-rich motif is one of three nuclear localization signals that determine unidirectional transport of the mineralocorticoid receptor to the nucleus. Journal of Biological Chemistry 2005, 280: 17549
(149) Wang Y, Wang Z, Du G, Hua Z, Liu L, Li J, Chen J. Enhancement of alkaline polygalacturonate lyase production in recombinant Pichia pastoris according to the ratio of methanol to cell concentration. Bioresour Technol 2009, 100: 1343-1349
(150) Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991, 173: 697-703
(151) Wisotzkey JD, Jurtshuk P, Jr., Fox GE, Deinhard G, Poralla K. Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 1992, 42: 263-269
(152) Wu S, Liu B, Zhang X (2006) Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific. Appl Microbiol Biotechnol 72:1210?1216. DOI: 10.1007/s00253-006-0416-4
(153) Wyss M, Pasamontes L, Friedlein A, Remy R, Tessier M, Kronenberger A, Middendorf A, Lehmann M, Schnoebelen L, Rothlisberger U. Biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance. Applied and Environmental Microbiology 1999, 65: 359
(154) Xiong AS, Peng RH, Li X, Fan HQ, Yao QH, Guo MJ, Zhang SL. Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 2003, 35: 154-160
(155) Xu P, Harvey AJ, Fincher GB. Heterologous expression of cDNAs encoding barley (Hordeum vulgare) (1-->3)-beta-glucanase isoenzyme GV. FEBS Lett 1994, 348: 206-210
(156) Xu P, Raden D, Doyle FJ, 3rd, Robinson AS. Analysis of unfolded protein response during single-chain antibody expression in Saccaromyces cerevisiae reveals different roles for BiP and PDI in folding. Metab Eng 2005, 7: 269-279
(157) Yamazaki K, Teduka H, Inoue N, Shinano H. Specific primers for detection of Alicyclobacillus acidoterrestris by RT-PCR. Lett Appl Microbiol 1996, 23: 350-354
(158) Yin Y, Baidoo S, Schulze H, Simmins P. Effects of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with [beta]-glucanase and xylanase on the physiological status of the gastrointestinal tract and nutrient digestibility of weaned pigs. Livestock Production Science 2001, 71: 97-107
(159) Yuan TZ, Yao B, Luo HY, Wang YR, Wu NF, Fan YL. Expression of acidophilic alpha-amylase from Alicyclobacillus acidocaldarius. Sheng Wu Gong Cheng Xue Bao 2005, 21: 78-83
(160) Zhang AL, Luo JX, Zhang TY, Chen SC, Guan WJ. Constitutive expression of human angiostatin in Pichia pastoris using the GAP promoter. Yi Chuan Xue Bao 2004, 31: 552-557
(161) Zhang AL, Luo JX, Zhang TY, Pan YW, Tan YH, Fu CY, Tu FZ. Recent advances on the GAP promoter derived expression system of Pichia pastoris. Mol Biol Rep 2009, 36: 1611-1619
(162)陈冠军,秦梦华。纤维素酶脱墨机理的研究进展。生物工程进展2001,21: 17-22
(163)冯德芹,杨苏声,极端微生物蛋白质组学的研究现状。微生物学报2008,48: 1675-1680
(164)顾琪萍,勇强,叶汉玲,尤纪雪。纤维素酶与木聚糖酶混合用于脱墨的研究。林产化学与工业2003,23: 19-22
(165)顾琪萍,尤纪雪,勇强,余世袁。脂肪酶和纤维素酶/木聚糖酶混合用于ONP脱墨。中国造纸2004,23: 7-9
(166)和致中,彭谦。云南温泉高温菌的研究:Ⅶ腾冲酸性高温温泉中的极端嗜热性芽孢杆菌。微生物学报1989,29: 161-165
(167)胡贻椿,岳田利,袁亚宏,高振鹏。果汁中脂环酸芽孢杆菌(Alicyclobacillus spp.)的危害及其控制。食品科学2008,29: 364-368
(168)黄茂福。染整用酶制剂的研究与开发现状(Ⅱ)。印染助剂2004,21: 1-4
(169)李宁。链霉菌来源的木聚糖降解酶相关基因的克隆及酶学性质研究。中国农业科学院2009。
(170)李平凡,邱玉美,吴海峰。双酶法生产葡萄糖工艺优化研究。现代食品科技2008,24: 262-264
(171)吕保国。双酶法葡萄糖的生产改进。发酵科技通讯1992,21: 20-21
(172)罗会颖。极端嗜酸真菌Bispora sp.MEY-1胞外糖苷水解酶类的产酶分析及其相关基因的克隆与表达。中国农业科学院2008。
(173)玛丽,蒂勒利,张明慧。酶在餐具洗涤剂中的应用。日用化学品科学2002,25。
(174)聂志扬,肖飞,郭健。DNA测序技术与仪器的发展。中国医疗器械信息2009,15: 13-16。
(175)曲音波。纺织,造纸工业用酶的研制。精细与专用化学品2002,10: 14-15。
(176)佘元莉,李秀婷,宋焕禄,马家津,吕跃钢。微生物木聚糖酶的研究进展。中国酿造2009: 1-4。
(177)孙玉英,王瑞明。β-葡聚糖酶的研究进展。山东商业职业技术学院学报2002,2: 11-13。
(178)王梅,焦必宁,薛杨。果汁中脂环酸芽孢杆菌研究进展。食品与发酵工业2009: 96-102。
(179)王楠,马荣山。耐高温α-淀粉酶的研究进展。中兽医医药杂志2007,26: 68-69。
(180)谢响明,何晓青。微生物的木聚糖酶及其生物漂白研究进展。北京林业大学学报2003, 25:111-116。
(181)徐春,花芸淀粉酶在表面施胶中的应用。黑龙江造纸2006,34: 44-44
(182)徐清。洗涤剂中的淀粉酶。日用化学品科学1997, 5。
(183)尤新。淀粉糖品生产与应用手册。1997。
(184)岳田利,胡贻椿,袁亚宏,高振鹏。脂环酸芽孢杆菌(Alicyclobacillus)分离鉴定研究进展。食品科学2008,29: 487-492
(185)张素风陈鹏,郑冬颖。木聚糖酶用于旧书刊纸脱墨的研究。中国造纸2005, 24: 13-16
(186)朱旭芬,曾云中。生物体内泛醌的种类及合成条件的探讨。浙江大学学报:理学版2000,27: 324-328
(2) Albuquerque L, Rainey FA, Chung AP, Sunna A, Nobre MF, Grote R, Antranikian G. Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of Sao Miguel in the Azores. Int J Syst Evol Microbiol 2000, 50: 451-457
(3) Almirall M, Esteve-Garcia E. In vitro stability of aβ-glucanase preparation from Trichoderma longibrachiatum and its effect in a barley based diet fed to broiler chicks.Время, 2009,54: 149-158
(4) An H, Peavy T, Hedrick J, Lebrilla C. Determination of N-glycosylation sites and site heterogeneity in glycoproteins. Anal Chem 2003, 75: 5628-5637
(5) Aono R, Hammura M, Yamamoto M, Asano T. Isolation of extracellular 28- and 42-kilodalton beta-1,3-glucanases and comparison of three beta-1,3-glucanases produced by Bacillus circulans IAM1165. Appl Environ Microbiol 1995, 61: 122-129
(6) Aquino AC, Jorge JA, Terenzi HF, Polizeli ML. Studies on a thermostable alpha-amylase from the thermophilic fungus Scytalidium thermophilum. Appl Microbiol Biotechnol 2003, 61: 323-328
(7) Bamforth CW. The adaptability, purification and properties of exo-beta-1,3-glucanase from the fungus Trichoderma reesei. Biochem J 1980, 191: 863-866
(8) Belev TN, Singh M, McCarthy JE. A fully modular vector system for the optimization of gene expression in Escherichia coli. Plasmid 1991, 26: 147-150
(9) Bevilacqua A, Sinigaglia M, Corbo MR. Alicyclobacillus acidoterrestris: new methods for inhibiting spore germination. Int J Food Microbiol 2008, 125: 103-110
(10) Borlinghaus A, Engel R. Alicyclobacillus incidence in commercial apple juice concentrate (AJC) supplies-method development and validation. Fruit processing 1997, 7: 262-266
(11) Buliga GS, Brant DA, Fincher GB. The sequence statistics and solution conformation of a barley (1-3, 1-4)-beta-D-glucan. Carbohydr Res 1986, 157: 139-156
(12) Burnell T, Cromwell G, Stahly T. Effects of dried whey and copper sulfate on the growth responses to organic acid in diets for weanling pigs. Journal of animal science 1988, 66: 1100
(13) Campbell LL. Purification and properties of an alpha-amylase from facultative thermophilic bacteria. Arch Biochem 1955, 54: 154-161
(14) Cebe R, Geiser M. Rapid and easy thermodynamic optimization of the 5'-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli. Protein Expr Purif 2006, 45: 374-380
(15) Celestino, K., Cunha, R., Felix, C., 2006. Characterization of aβ-glucanase produced byRhizopus microsporus var. microsporus, and its potential for application in the brewing industry. BMC biochemistry, 7, 23
(16) Celik E, Calik P, Oliver SG. Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast 2009, 26: 473-484
(17) Chadha BS, Rubinder K, Saini HS. Constitutive alpha-amylase producing mutant and recombinant haploid strains of thermophilic fungus Thermomyces lanuginosus. Folia Microbiol (Praha) 2005, 50: 133-140
(18) Chae HJ, Delisa MP, Cha HJ, Weigand WA, Rao G, Bentley WE. Framework for online optimization of recombinant protein expression in high-cell-density Escherichia coli cultures using GFP-fusion monitoring. Biotechnol Bioeng 2000, 69: 275-285
(19) Chang GD, Li ZL, Qin JY, Ma CQ, Luo YZ, Xu P. Optimization of fermentation of recombinant human Endostatin (rh-Endostatin) expression in Escherichia coli. Sheng Wu Gong Cheng Xue Bao 2005, 21: 662-666
(20) Chang SS, Kang DH. Alicyclobacillus spp. in the fruit juice industry: history, characteristics, and current isolation/detection procedures. Crit Rev Microbiol 2004, 30: 55-74
(21) Chang SW, Lee GC, Shaw JF. Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase. J Agric Food Chem 2006, 54: 815-822
(22) Clark S, Muslin E, Henson C. Effect of adding and removing N-glycosylation recognition sites on the thermostability of barley {alpha}-glucosidase. Protein Engineering Design and Selection 2004, 17: 245
(23) Connor CJ, Luo H, McSpadden Gardener BB, Wang HH. Development of a real-time PCR-based system targeting the 16S rRNA gene sequence for rapid detection of Alicyclobacillus spp. in juice products. International Journal of Food Microbiology 2005, 99: 229-235
(24) Cotelle N, Lohez M, Cotelle P, Hénichart J. Conformational study of the threonine-rich C-terminal pentapeptide of peptide T. Biochemical and biophysical research communications 1990, 171: 596-602
(25) Dai X. Yeast Pichia pastoris-a notable heterologous gene expression system. Wei Sheng Wu Xue Bao 1997, 37: 483-485
(26) Danicke S, Vahjen W, Simon O, Jeroch H. Effects of dietary fat type and xylanase supplementation to rye-based broiler diets on selected bacterial groups adhering to the intestinal epithelium on transit time of feed, and on nutrient digestibility. Poult Sci 1999, 78: 1292-1299
(27) Darland G, Brock T. Bacillus acidocaldarius sp. nov., an acidophilic thermophilic spore-forming bacterium. Microbiology 1971, 67: 9
(28) de la Cruz J, Pintor-Toro JA, Benitez T, Llobell A. Purification and characterization of an endo-beta-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism. J Bacteriol 1995, 177: 1864-1871
(29) Deinhard G, Blanz P, Poralla K, Altan E. Bacillus acidoterrestris sp. nov., a new thermotolerantacidophile isolated from different soils. Systematic and Applied Microbiology 1987, 10: 47-53
(30) Delcher A, Harmon D, Kasif S. Improved microbial gene identification with GLIMMER. Nucleic acids research 1999, 27: 4636
(31) Dersjant-Li Y, Schulze H, Schrama JW, Verreth JA, Verstegen MW. Feed intake, growth, digestibility of dry matter and nitrogen in young pigs as affected by dietary cation-anion difference and supplementation of xylanase. J Anim Physiol Anim Nutr (Berl) 2001, 85: 101-109
(32) Di Carlo FJ, Redfern S. alpha-Amylase from Bacillus subtilis; effects of inhibitors upon liquefaction and dextrinization. Arch Biochem 1948, 17: 1
(33) Dybkjaer L, Jacobsen A, Togersen F, et al. Eating and drinking activity of newly weaned piglets: Effects of individual characteristics, social mixing, and addition of extra zinc to the feed. Journal of animal science 2006, 84: 702
(34) Eckert K, Ernst HA, Schneider E, Larsen S, Lo Leggio L. Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA. Acta Crystallogr D Biol Crystallogr 2003, 59: 139-141
(35) Eckert K, Schneider E. A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases. Eur J Biochem 2003, 270: 3593-3602
(36) Eckert K, Zielinski F, Lo Leggio L, Schneider, E. Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009. Appl Microbiol Biotechnol 2002, 60: 428-436
(37) Eckert K, Schneider E. A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases. Eur J Biochem 2003, 270: 3593-602
(38) Erasmus JA. Some features of coagulase positive staphylococci from bovine milk. 1. Carbohydrate metabolism: comparison of conventional techniques and the API 50 CH system. Onderstepoort J Vet Res 1985, 52: 25-29
(39) Fidler AE, Lin JS, Lun S, Ng Chie W, Western A, Stent V, McNatty KP. Production of biologically active tethered ovine FSHbetaalpha by the methylotrophic yeast Pichia pastoris. J Mol Endocrinol 2003, 30: 213-225
(40) Gabert V, Sauer W, Li S, Fan M. Exocrine pancreatic secretions in young pigs fed diets containing faba beans (Vicia faba) and peas (Pisum sativum): concentrations and flows of total, protein-bound and free amino acids. Journal of the Science of Food and Agriculture 1999, 70: 256-262
(41) Gangadharan D, Nampoothiri KM, Sivaramakrishnan S, Pandey A. Biochemical characterization of raw-starch-digesting alpha amylase purified from Bacillus amyloliquefaciens. Appl Biochem Biotechnol 2009, 158: 653-662
(42) Garrett J, Kretz K, O'Donoghue E, Kerovuo J, Kim W, Barton N, Hazlewood G, Short J, Robertson D, Gray K. Enhancing the thermal tolerance and gastric performance of a microbialphytase for use as a phosphate-mobilizing monogastric-feed supplement. Applied and Environmental Microbiology 2004, 70: 3041-3046
(43) Gessesse A. Purification and properties of two thermostable alkaline xylanases from an alkaliphilic bacillus sp. Appl Environ Microbiol 1998, 64: 3533-3535
(44) Gianinetti A. A theoretical framework for beta-glucan degradation during barley malting. Theory Biosci 2009, 128: 97-108.
(45) Goto K, Matsubara H, Mochida K, Matsumura T, Hara Y, Niwa M, Yamasato K. Alicyclobacillus herbarius sp. nov., a novel bacterium containing omega-cycloheptane fatty acids, isolated from herbal tea. Int J Syst Evol Microbiol 2002, 52: 109-113
(46) Graham H, Fadel JG, Newman CW, Newman RK. Effect of pelleting and beta-glucanase supplementation on the ileal and fecal digestibility of a barley-based diet in the pig. J Anim Sci 1989, 67: 1293-1298
(47) Groenewald WH, Gouws PA, Witthuhn RC. Isolation and identification of species of Alicyclobacillus from orchard soil in the Western Cape, South Africa. Extremophiles 2008, 12: 159-163
(48) Groenewald WH, Gouws PA, Witthuhn RC. Isolation, identification and typification of Alicyclobacillus acidoterrestris and Alicyclobacillus acidocaldarius strains from orchard soil and the fruit processing environment in South Africa. Food Microbiol 2009, 26: 71-76
(49) Guo X, You XY, Liu LJ, Zhang JY, Liu SJ, Jiang CY. Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine. Int J Syst Evol Microbiol 2009, 59: 2415-2420
(50) Haraldsson A, Veide J, Andlid T, Alminger M, Sandberg A. Degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. Journal of agricultural and food chemistry 2005, 53: 5438-5444
(51) Hasegawa S, Nordin JH. Enzymes that hydrolyze fungal cell wall polysaccharides. I. Purification and properties of an endo-alpha-D-(1,3)-glucanase from Trichoderma. J Biol Chem 1969, 244: 5460-5470
(52) Hayashida S, Teramoto Y, Inoue T. Production and Characteristics of Raw-Potato-Starch-Digesting alpha-Amylase from Bacillus subtilis 65. Appl Environ Microbiol 1988, 54: 1516-1522
(53) Hayashida S, Teramoto Y. Production and Characteristics of Raw-Starch-Digesting alpha-Amylase from a Protease-Negative Aspergillus ficum Mutant. Appl Environ Microbiol 1986, 52: 1068-1073
(54) Hien NH, Fleet GH. Separation and characterization of six (1 leads to 3)-beta-glucanases from Saccharomyces cerevisiae. J Bacteriol 1983, 156: 1204-1213
(55) Hirsch K, Simon O, Vahjen W. Influence of a xylanase feed additive on Lactobacillus species in the jejunum of piglets. Berl Munch Tierarztl Wochenschr 2006, 119: 486-492
(56) Hu S, Li L, Qiao J, Guo Y, Cheng L, Liu J. Codon optimization, expression, and characterizationof an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris. Protein Expr Purif 2006, 47: 249-257
(57) Hwang KY, Song HK, Chang C, Lee J, Lee SY, Kim KK, Choe S. Crystal structure of thermostable alpha-amylase from Bacillus licheniformis refined at 1.7 A resolution. Mol Cells 1997, 7: 251-258
(58) Inagaki K, Nakahira K, Mukai K, Tamura T, Tanaka H. Gene cloning and characterization of an acidic xylanase from Acidobacterium capsulatum. Biosci Biotechnol Biochem 1998, 62: 1061?1067.
(59) Inborr J, Puhakka J, Bakker JG, Van der Meulen J. Beta-glucanase and xylanase activities in stomach and ileum of growing pigs fed wheat bran based diets with and without enzyme treatment. Arch Tierernahr 1999, 52: 263-274
(60) Jeang CL, Chen LS, Chen MY, Shiau RJ. Cloning of a gene encoding raw-starch-digesting amylase from a Cytophaga sp. and its expression in Escherichia coli. Appl Environ Microbiol 2002, 68: 3651-3654
(61) Jeang CL, Lee YH, Chang LW. Purification and characterization of a raw-starch digesting amylase from a soil bacterium-Cytophaga sp. Biochem Mol Biol Int 1995, 35: 549-557
(62) Jiang ZB, Song HT, Ma LX. Secreted expression of Bacillus pumilus xylanase gene in Pichia pastoris and study on enzymatic properties. Sheng Wu Gong Cheng Xue Bao 2003, 19: 50-55
(63) Joao H, Scragg I, Dwek R. Effects of glycosylation on protein conformation and amide proton exchange rates in RNase B. FEBS Letters 1992, 307: 343-346
(64) Jonsson LJ, Saloheimo M, Penttila M. Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr Genet 1997, 32: 425-430
(65) Joseleau J, Comtat J, Ruel K. Chemical structure of xylans and their interaction in the plant cell walls. Progress in Biotechnology 1992, 7: 1-5
(66) Jungo C, Marison I, von Stockar U. Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures. J Biotechnol 2007, 128: 824-837
(67) Kanehisa M, Araki M, Goto S. KEGG for linking genomes to life and the environment. Nucleic acids research 2008, 36: D480
(68) Karlin S, Bucher P, Brendel V, et al. Statistical methods and insights for protein and DNA sequences. Annual review of biophysics and biophysical chemistry 1991, 20: 175-203
(69) Khatri NK, Hoffmann F. Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol Bioeng 2006, 93: 871-879
(70) Kim CH, Sata H, Taniguchi H, Maruyama Y. Cloning and expression of raw-starch-digesting alpha-amylase gene from Bacillus circulans F-2 in Escherichia coli. Biochim Biophys Acta 1990, 1048: 223-230
(71) Kim J, Nanmori T, Shinke R. Thermostable, Raw-Starch-Digesting Amylase from Bacillus stearothermophilus. Appl Environ Microbiol 1989, 55: 1638-1639
(72) Kim KH, Kim YO, Ko BS, Youn HJ, Lee DS. Over-expression of the gene (bglBC1) from Bacillus circulans encoding an endo-beta-1,3-1,4-glucanase useful for the preparation of oligosaccharides from barley beta-glucan. Biotechnol Lett 2004, 26: 1749-1755
(73) Kotake T, Nakagawa N, Takeda K, Sakurai N. Purification and characterization of wall-bound exo-1,3-beta-D-glucanase from barley (Hordeum vulgare L) seedlings. Plant Cell Physiol 1997, 38: 194-200
(74) Kubicek CP. beta-Glucosidase excretion by Trichoderma pseudokoningii: correlation with cell wall bound beta-1.3-glucanase activities. Arch Microbiol 1982, 132: 349-354
(75) Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680-685
(76) Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 1985, 82: 6955-6959
(77) Lee CC, Kibblewhite-Accinelli RE, Wagschal K, Robertson GH, Wong DW. Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library. Extremophiles 2006, 10: 295-300
(78) Lee CK, Darah I, Ibrahim CO. Enzymatic deinking of laser printed office waste papers: some governing parameters on deinking efficiency. Bioresour Technol 2007, 98: 1684-1689
(79) Lee S, Oneda H, Minoda M, Tanaka A, Inouye K. Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis alpha-amylases and insights into engineering alpha-amylase variants active under acidic conditions. J Biochem 2006, 139: 997-1005
(80) Lee TH, Lim PO, Lee YE. Cloning, characterization, and expression of xylanase A gene from Paenibacillus sp. DG-22 in Escherichia coli. J Microbiol Biotechnol 2007, 17: 29-36
(81) Li N, Shi P, Yang P, Wang Y, Luo H, Bai Y, Zhou Z, et al. A xylanase with high pH stability from Streptomyces sp. S27 and its carbohydrate-binding module with/without linker-region-truncated versions. Appl Microbiol Biotechnol 2009, 83:99-107
(82) Li S, Sauer WC, Huang SX, Gabert VM. Effect of beta-glucanase supplementation to hulless barley- or wheat-soybean meal diets on the digestibilities of energy, protein, beta-glucans, and amino acids in young pigs. J Anim Sci 1996, 74: 1649-1656
(83) Li Y, Lu J, Gu G, Shi Z, Mao Z. Mathematical modeling for prediction of endo-xylanase activity and arabinoxylans concentration during mashing of barley malts for brewing. Biotechnol Lett 2004, 26: 779-785
(84) Lim HK, Choi SJ, Kim KY, Jung KH. Dissolved-oxygen-stat controlling two variables for methanol induction of rGuamerin in Pichia pastoris and its application to repeated fed-batch. Appl Microbiol Biotechnol 2003, 62: 342-348
(85) Liu R, Lin Q, Sun Y, Qiu Y, Xu T, Ding H, Guo X. Optimization of fermentation conditions for maximal recombinant hepatitis B surface antigen particle production in Pichia pastoris. Sheng Wu Gong Cheng Xue Bao 2008, 24: 2098-2105
(86) Liu XD, Xu Y. A novel raw starch digesting alpha-amylase from a newly isolated Bacillus sp. YX-1: purification and characterization. Bioresour Technol 2008, 99: 4315-4320
(87) Liu YG, Mitsukawa N, Oosumi T, Whittier RF. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 1995, 8: 457-463
(88) Loginova LG, Karpukhina S. Some features of alpha-amylase in thermophilic bacteria. Dokl Akad Nauk SSSR 1968, 180: 225-228
(89) Luo H, Yang J, Yang P, et al. Gene cloning and expression of a new acidic family 7 endo-beta-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1. Appl Microbiol Biotechnol, 85: 1015-1023
(90) Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22: 249-270
(91) Machius M, Wiegand G, Huber R. Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution. J Mol Biol 1995, 246: 545-559
(92) Manimaran A, Kumar KS, Permaul K, Singh S. Hyper production of cellulase-free xylanase by Thermomyces lanuginosus SSBP on bagasse pulp and its application in biobleaching. Appl Microbiol Biotechnol 2009, 81: 887-893
(93) Marques S, Pala H, Alves L, Amaral-Collaco MT, Gama FM, Girio FM. Characterisation and application of glycanases secreted by Aspergillus terreus CCMI 498 and Trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper. J Biotechnol 2003, 100: 209-219
(94) Marshall JJ. Degradation of barley glucan by a purified (1 yields 4)-beta-D-glucanase from the snail, Helix pomatia. Carbohydr Res 1975, 42: 203-207
(95) Martin KL, McDougall BM, Unkles SE, Seviour RJ. The three beta-1,3-glucanases from Acremonium blochii strain C59 appear to be encoded by separate genes. Mycol Res 2006, 110: 66-74
(96) Mathlouthi N, Saulnier L, Quemener B, Larbier M. Xylanase, beta-glucanase, and other side enzymatic activities have greater effects on the viscosity of several feedstuffs than xylanase and beta-glucanase used alone or in combination. J Agric Food Chem 2002, 50: 5121-5127
(97) Matsubara H, Goto K, Matsumura T, Mochida K, Iwaki M, Niwa M, Yamasato K. Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages. Int J Syst Evol Microbiol 2002, 52: 1681-1685
(98) Mattanovich D, Weik R, Thim S, Kramer W, Bayer K, Katinger H. Optimization of recombinant gene expression in Escherichia coli. Ann N Y Acad Sci 1996, 782: 182-190
(99) Matzke J, Schwermann B, Bakker EP. Acidostable and acidophilic proteins: the example of the alpha-amylase from Alicyclobacillus acidocaldarius. Comp Biochem Physiol A Physiol 1997, 118: 475-479
(100) McCarthy T, Hanniffy O, Savage AV, Tuohy MG. Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3-1,4-linkedglucans. Int J Biol Macromol 2003, 33: 141-148
(101) McQueen R, Van Soest P. Fungal cellulase and hemicellulase prediction of forage digestibility. Journal of Dairy Science 1975, 58: 1482
(102) Mikami B, Adachi M, Kage T, Sarikaya E, Nanmori T, Shinke R, Utsumi S. Structure of raw starch-digesting Bacillus cereus beta-amylase complexed with maltose. Biochemistry 1999, 38: 7050-7061
(103) Minekus M, Marteau P, Havenaar R, HUIS I. A multicompartmental dynamic computer-controlled model simulating the stomach and small intestine. ATLA Alternatives to laboratory animals 1995, 23: 197-209
(104) Monfort A, Blasco A, Prieto JA, Sanz P. Combined Expression of Aspergillus nidulans Endoxylanase X24 and Aspergillus oryzae (alpha)-Amylase in Industrial Baker's Yeasts and Their Use in Bread Making. Appl Environ Microbiol 1996, 62: 3712-3715
(105) Monma M, Kainuma K. Heterogeneity of the glucoamylase components of the raw-starch-digesting amylase from Chalara paradoxa. Carbohydr Res 1992, 227: 385-388
(106) Morana A, Esposito A, Maurelli L, Ruggiero G, Ionata E, Rossi M, La Cara F.A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius. Protein Pept Lett 2008, 15: 1017-1021
(107) Mountfort DO, Asher RA. Production of alpha-Amylase by the Ruminal Anaerobic Fungus Neocallimastix frontalis. Appl Environ Microbiol 1988, 54: 2293-2299
(108) Nakamura T, Namba H, Ohmoto T, Liu Y, Hirata D, Miyakawa T. Cloning and characterization of the Saccharomyces cerevisiae SVS1 gene which encodes a serine-and threonine-rich protein required for vanadate resistance. Gene 1995, 165: 25-29
(109) Niwa M, Kuriyama A. A. acidoterrestris rapid detection kit. Fruit processing 2003: 328-331
(110) Ohya T, Ohyama M, Kobayashi K. Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation. Biotechnol Bioeng 2005, 90: 876-887
(111) Oka C, Tanaka M, Muraki M, Harata K, Suzuki K, Jigami Y. Human lysozyme secretion increased by alpha-factor pro-sequence in Pichia pastoris. Biosci Biotechnol Biochem 1999, 63: 1977-1983
(112) Oliveira R, Clemente JJ, Cunha AE, Carrondo MJ. Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation. J Biotechnol 2005, 116: 35-50
(113) Pala H, Mota M, Gama FM. Enzymatic versus chemical deinking of non-impact ink printed paper. J Biotechnol 2004, 108: 79-89
(114) Yang P, Shi P, Wang Y, Bai Y, Meng K, Luo H, Yuan T, Yao B. Cloning and Overexpression of a Paenibacillusβ-Glucanase in Pichia pastoris: Purification and Characterization of the Recombinant Enzyme. Journal of microbiology and biotechnology 2007, 17: 58-66
(115) Penayanez A, Vilches A, Moralara RJ. Application of Somogyi's Method to the Determination ofAmylase in the Urine. Rev Clin Esp 1963, 91: 93-96
(116) Plantz BA, Sinha J, Villarete L, Nickerson KW, Schlegel VL. Pichia pastoris fermentation optimization: energy state and testing a growth-associated model. Appl Microbiol Biotechnol 2006, 72: 297-305
(117) Ponte PI, Lordelo MM, Guerreiro CI, Soares MC, Mourao JL, Crespo JP, Crespo DG, et al. Crop beta-glucanase activity limits the effectiveness of a recombinant cellulase used to supplement a barley-based feed for free-range broilers. Br Poult Sci 2008, 49: 347-359
(118) Rajendran S, Radha C, Prakash V. Mechanism of solvent-induced thermal stabilization of alpha-amylase from Bacillus amyloliquefaciens. Int J Pept Protein Res 1995, 45: 122-128
(119) Ramchuran SO, Holst O, Karlsson EN. Effect of postinduction nutrient feed composition and use of lactose as inducer during production of thermostable xylanase in Escherichia coli glucose-limited fed-batch cultivations. J Biosci Bioeng 2005, 99: 477-484
(120) Ramon R, Feliu JX, Cos O, Montesinos JL, Berthet FX, Valero F. Improving the monitoring of methanol concentration during high cell density fermentation of Pichia pastoris. Biotechnol Lett 2004, 26: 1447-1452
(121) Rapp P. Formation, separation and characterization of three beta-1,3-glucanases from Sclerotium glucanicum. Biochim Biophys Acta 1992, 1117: 7-14
(122) Rauceo J, De Armond R, Otoo H, Kahn P, Klotz S, Gaur N, Lipke P. Threonine-rich repeats increase fibronectin binding in the Candida albicans adhesin Als5p. Eukaryotic Cell 2006, 5: 1664
(123) Richardson TH, Tan X, Frey G, Callen W, Cabell M, Lam D, Macomber J, et al. A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable alpha-amylase. J Biol Chem 2002, 277: 26501-26507
(124) Saha BC, Lecureux LW, Zeikus JG. Raw starch adsorption-desorption purification of a thermostable beta-amylase from Clostridium thermosulfurogenes. Anal Biochem 1988, 175: 569-572
(125) Saito N. alpha-Amylase inhibitor from fungus Cladosporium herbarum F-828. J Biol Chem 1982, 257: 3120-3125
(126) Schwermann B, Pfau K, Liliensiek B, Schleyer M, Fischer T, Bakker EP. Purification, properties and structural aspects of a thermoacidophilic alpha-amylase from Alicyclobacillus acidocaldarius atcc 27009. Insight into acidostability of proteins. Eur J Biochem 1994, 226: 981-991
(127) Shaw A, Bott R, Day A. Protein engineering ofα-amylase for low pH performance. Current opinion in biotechnology 1999, 10: 349-352
(128) Simbahan J, Drijber R, Blum P. Alicyclobacillus vulcanalis sp. nov., a thermophilic, acidophilic bacterium isolated from Coso Hot Springs, California, USA. Int J Syst Evol Microbiol 2004, 54: 1703-1707
(129) Simpson HD, Haufler UR, Daniel RM. An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga. Biochem J 1991, 277: 413-417
(130) Singh VK, Mangalam AK, Dwivedi S, Naik S. Primer premier: program for design of degenerate primers from a protein sequence. Biotechniques 1998, 24: 318-319
(131) Spllttstoesser D, Churey J, Lee C. Growth characteristics of aciduric sporeforming bacilli isolated from fruit juices. Journal of Food Protectionand 1994, 174: 1080-1083
(132) Suzek B, Ermolaeva M, Schreiber M. A probabilistic method for identifying start codons in bacterial genomes. Bioinformatics 2001, 17: 1123
(133) Tabernero C, Sánchez-Torres J, Pérez P, Santamaría RI Cloning and DNA sequencing of xyaA, a gene encoding an endo-beta-1,4-xylanase from an alkalophilic Bacillus strain (N137). Appl Environ Microbiol 1995, 61:2420?2424.
(134) Tager M, Kroning H, Thiel U, Ansorge S. Membrane-bound proteindisulfide isomerase (PDI) is involved in regulation of surface expression of thiols and drug sensitivity of B-CLL cells. Exp Hematol 1997, 25: 601-607
(135) Tan LU, Mayers P, Illing M, Saddler JN. The copurification of beta-glucosidase, beta-xylosidase, and 1,3-beta-glucanase in two separate enzyme complexes isolated from Trichoderma harzianum E58. Biochem Cell Biol 1987, 65: 822-832
(136) Tanaka A, Hoshino E. Similarities between the thermal inactivation kinetics of Bacillus amyloliquefaciens alpha-amylase in an aqueous solution of sodium dodecyl sulphate and the kinetics in the solution of anionic-phospholipid vesicles. Biotechnol Appl Biochem 2003, 38: 175-181
(137) Tangarone B, Royer JC, Nakas JP. Purification and Characterization of an Endo-(1,3)-beta-D-Glucanase from Trichoderma longibrachiatum. Appl Environ Microbiol 1989, 55: 177-184
(138) Telenius H, Carter NP, Bebb CE, Nordenskjold M, Ponder BA, Tunnacliffe A. Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 1992, 13: 718-725
(139) Teng D, Fan Y, Yang YL, Tian ZG, Luo J, Wang JH. Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Appl Microbiol Biotechnol 2007, 74: 1074-1083
(140) Thornton CR. Use of monoclonal antibodies to quantify the dynamics of alpha-galactosidase and endo-1,4-beta-glucanase production by Trichoderma hamatum during saprotrophic growth and sporulation in peat. Environ Microbiol 2005, 7: 737-749
(141) Tricarico JM, Dawson KA. Influence of supplemental endoglucanase or xylanase on volatile fatty acid production from ruminant feed by ruminal in vitro cultures. Arch Anim Nutr 2005, 59: 325-334
(142) Tsuruoka N, Isono Y, Shida O, Hemmi H, Nakayama T, Nishino T. Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan. Int J Syst Evol Microbiol 2003, 53: 1081-1084
(143) Violet M, Meunier JC. Kinetic study of the irreversible thermal denaturation of Bacilluslicheniformis alpha-amylase. Biochem J 1989, 263: 665-670
(144) Von Wettstein D, Warner J, Kannangara CG. Supplements of transgenic malt or grain containing (1,3-1,4)-beta-glucanase increase the nutritive value of barley-based broiler diets to that of maize. Br Poult Sci 2003, 44: 438-449
(145) Walker M, Phillips CA. The effect of preservatives on Alicyclobacillus acidoterrestris and Propionibacterium cyclohexanicum in fruit juice. Food Control 2008, 19: 974-981
(146) Walls I, Chuyate R. Spoilage of fruit juices by Alicyclobacillus acidoterrestris: Alicyclobacillus in the food industry. Food Australia 2000, 52: 286-288
(147) Walsh GA, Murphy RA, Killeen GF, Headon DR, Power RF. Technical note: detection and quantification of supplemental fungal beta-glucanase activity in animal feed. J Anim Sci 1995, 73: 1074-1076
(148) Walther R, Atlas E, Carrigan A, et al. A serine/threonine-rich motif is one of three nuclear localization signals that determine unidirectional transport of the mineralocorticoid receptor to the nucleus. Journal of Biological Chemistry 2005, 280: 17549
(149) Wang Y, Wang Z, Du G, Hua Z, Liu L, Li J, Chen J. Enhancement of alkaline polygalacturonate lyase production in recombinant Pichia pastoris according to the ratio of methanol to cell concentration. Bioresour Technol 2009, 100: 1343-1349
(150) Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991, 173: 697-703
(151) Wisotzkey JD, Jurtshuk P, Jr., Fox GE, Deinhard G, Poralla K. Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 1992, 42: 263-269
(152) Wu S, Liu B, Zhang X (2006) Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific. Appl Microbiol Biotechnol 72:1210?1216. DOI: 10.1007/s00253-006-0416-4
(153) Wyss M, Pasamontes L, Friedlein A, Remy R, Tessier M, Kronenberger A, Middendorf A, Lehmann M, Schnoebelen L, Rothlisberger U. Biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance. Applied and Environmental Microbiology 1999, 65: 359
(154) Xiong AS, Peng RH, Li X, Fan HQ, Yao QH, Guo MJ, Zhang SL. Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 2003, 35: 154-160
(155) Xu P, Harvey AJ, Fincher GB. Heterologous expression of cDNAs encoding barley (Hordeum vulgare) (1-->3)-beta-glucanase isoenzyme GV. FEBS Lett 1994, 348: 206-210
(156) Xu P, Raden D, Doyle FJ, 3rd, Robinson AS. Analysis of unfolded protein response during single-chain antibody expression in Saccaromyces cerevisiae reveals different roles for BiP and PDI in folding. Metab Eng 2005, 7: 269-279
(157) Yamazaki K, Teduka H, Inoue N, Shinano H. Specific primers for detection of Alicyclobacillus acidoterrestris by RT-PCR. Lett Appl Microbiol 1996, 23: 350-354
(158) Yin Y, Baidoo S, Schulze H, Simmins P. Effects of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with [beta]-glucanase and xylanase on the physiological status of the gastrointestinal tract and nutrient digestibility of weaned pigs. Livestock Production Science 2001, 71: 97-107
(159) Yuan TZ, Yao B, Luo HY, Wang YR, Wu NF, Fan YL. Expression of acidophilic alpha-amylase from Alicyclobacillus acidocaldarius. Sheng Wu Gong Cheng Xue Bao 2005, 21: 78-83
(160) Zhang AL, Luo JX, Zhang TY, Chen SC, Guan WJ. Constitutive expression of human angiostatin in Pichia pastoris using the GAP promoter. Yi Chuan Xue Bao 2004, 31: 552-557
(161) Zhang AL, Luo JX, Zhang TY, Pan YW, Tan YH, Fu CY, Tu FZ. Recent advances on the GAP promoter derived expression system of Pichia pastoris. Mol Biol Rep 2009, 36: 1611-1619
(162)陈冠军,秦梦华。纤维素酶脱墨机理的研究进展。生物工程进展2001,21: 17-22
(163)冯德芹,杨苏声,极端微生物蛋白质组学的研究现状。微生物学报2008,48: 1675-1680
(164)顾琪萍,勇强,叶汉玲,尤纪雪。纤维素酶与木聚糖酶混合用于脱墨的研究。林产化学与工业2003,23: 19-22
(165)顾琪萍,尤纪雪,勇强,余世袁。脂肪酶和纤维素酶/木聚糖酶混合用于ONP脱墨。中国造纸2004,23: 7-9
(166)和致中,彭谦。云南温泉高温菌的研究:Ⅶ腾冲酸性高温温泉中的极端嗜热性芽孢杆菌。微生物学报1989,29: 161-165
(167)胡贻椿,岳田利,袁亚宏,高振鹏。果汁中脂环酸芽孢杆菌(Alicyclobacillus spp.)的危害及其控制。食品科学2008,29: 364-368
(168)黄茂福。染整用酶制剂的研究与开发现状(Ⅱ)。印染助剂2004,21: 1-4
(169)李宁。链霉菌来源的木聚糖降解酶相关基因的克隆及酶学性质研究。中国农业科学院2009。
(170)李平凡,邱玉美,吴海峰。双酶法生产葡萄糖工艺优化研究。现代食品科技2008,24: 262-264
(171)吕保国。双酶法葡萄糖的生产改进。发酵科技通讯1992,21: 20-21
(172)罗会颖。极端嗜酸真菌Bispora sp.MEY-1胞外糖苷水解酶类的产酶分析及其相关基因的克隆与表达。中国农业科学院2008。
(173)玛丽,蒂勒利,张明慧。酶在餐具洗涤剂中的应用。日用化学品科学2002,25。
(174)聂志扬,肖飞,郭健。DNA测序技术与仪器的发展。中国医疗器械信息2009,15: 13-16。
(175)曲音波。纺织,造纸工业用酶的研制。精细与专用化学品2002,10: 14-15。
(176)佘元莉,李秀婷,宋焕禄,马家津,吕跃钢。微生物木聚糖酶的研究进展。中国酿造2009: 1-4。
(177)孙玉英,王瑞明。β-葡聚糖酶的研究进展。山东商业职业技术学院学报2002,2: 11-13。
(178)王梅,焦必宁,薛杨。果汁中脂环酸芽孢杆菌研究进展。食品与发酵工业2009: 96-102。
(179)王楠,马荣山。耐高温α-淀粉酶的研究进展。中兽医医药杂志2007,26: 68-69。
(180)谢响明,何晓青。微生物的木聚糖酶及其生物漂白研究进展。北京林业大学学报2003, 25:111-116。
(181)徐春,花芸淀粉酶在表面施胶中的应用。黑龙江造纸2006,34: 44-44
(182)徐清。洗涤剂中的淀粉酶。日用化学品科学1997, 5。
(183)尤新。淀粉糖品生产与应用手册。1997。
(184)岳田利,胡贻椿,袁亚宏,高振鹏。脂环酸芽孢杆菌(Alicyclobacillus)分离鉴定研究进展。食品科学2008,29: 487-492
(185)张素风陈鹏,郑冬颖。木聚糖酶用于旧书刊纸脱墨的研究。中国造纸2005, 24: 13-16
(186)朱旭芬,曾云中。生物体内泛醌的种类及合成条件的探讨。浙江大学学报:理学版2000,27: 324-328