痤疮灵颗粒及其拆方对金黄地鼠皮脂腺斑影响的实验研究
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摘要
目的：痤疮是一种青春期常见的毛囊皮脂腺的慢性炎症性疾病,表现为粉刺、丘疹、脓疱、结节和瘢痕等,好发于面背胸等富含皮脂腺的部位,但以颜面部居多,给患者的工作生活带来了不良影响,并危害患者的身心健康。本实验以金黄地鼠侧腹部皮脂腺斑为实验对象,以中医药理论为指导,通过测量皮脂腺斑的大小,观察皮脂腺斑的显微结构,阐明中药复方痤疮灵颗粒组方的合理性,找出复方中有效药味,为其在治疗痤疮中对性激素影响的物质基础及其作用机理研究做好更进一步基础工作。
方法：雄性金黄地鼠62只,重120～150g。正常饲养一周后,随机分为六组,空白组12只,全方组、拆方1组、拆方2组、拆方3组、阳性对照组各10只。
实验开始时先用电动剃须刀剃尽金黄地鼠双侧背部毛,充分暴露皮脂腺斑,在强光照射下,用游标卡尺测量双侧斑块的最大横径(DT)和最大纵径(DL)。灌胃时,各组金黄地鼠均按人的10倍剂量给药。全方组按金黄地鼠11g/kg生药的剂量给药,拆方1组、拆方2组、拆方3组分别按金黄地鼠6g/kg、2g/kg、3g/kg生药的剂量给药；阳性对照组按10mg/kg剂量给药；空白组灌服等量(1ml/100g)生理盐水。每日一次,连续灌胃4周。实验结束时再次用电动剃须刀剃尽金黄地鼠背部毛,充分暴露双侧皮脂腺斑,在强光照射下,用游标卡尺测量双侧斑块的最大横径和最大纵径。处死动物后取下金黄地鼠侧背部皮脂腺斑组织,切取约1.5cm*1.5cm组织块,放入10%甲醛固定液小瓶中固定,常规石蜡包埋、切片、HE染色,于光镜下观察皮脂腺斑的组织学变化。解剖各组金黄地鼠并称取其睾丸重量,计算睾丸系数。
结果：
1.皮脂腺斑大小：痤疮灵全方组、拆方1组、拆方2组、拆方3组及阳性对照组皮脂腺斑较治疗前缩小,具有非常显著性差异或显著性差异(p<0.01或0.05),空白组皮脂腺斑与治疗前比较无显著性差异(p>0.05)；治疗后全方组、拆方1组、拆方2组及阳性对照组左侧皮脂腺斑面积较空白组明显缩小,具有非常显著性差异(p<0.01),拆方3组与空白组比较有显著性差异(p<0.05)；全方组、拆方1组、拆方2组右侧皮脂腺斑面积较空白组明显缩小,具有非常显著性差异(p<0.01),阳性对照组、拆方3组与空白组比较有显著性差异(p<0.05)。痤疮灵全方组、拆方1组、拆方2组、拆方3组皮脂腺斑大小与阳性对照组比较无显著性差异(p>0.05)；全方组、拆方1组、拆方2组、拆方3组皮脂腺斑大小组间比较无显著性差异(p>0.05)。
2.皮脂腺斑组织学结构：痤疮灵全方组、拆方1组、拆方2组、拆方3组及阳性对照组均较空白组皮脂腺松散,厚度变薄,皮脂腺腺叶变小；其中全方组、拆方2组较明显。皮脂腺厚度的比较：痤疮灵全方组皮脂腺厚度较空白组明显变薄,具有非常显著性差异(P<0.01)；拆方2组较空白组变薄,具有显著性差异(P<0.05)；拆方1组、拆方3组、阳性对照组与空白组比较,无显著性差异(P>0.05)。与阳性对照组比较,全方组、拆方1组、拆方2组、拆方3组均无显著性差异(P>0.05)。全方组、拆方1组、拆方2组及拆方3组皮脂腺厚度组间比较无显著性差异(P>0.05)。皮脂腺松散程度的比较：与空白组比较,痤疮灵全方组、拆方1组、拆方2组及阳性对照组均有非常显著性差异(P<0.01)；拆方3组具有显著性差异(P<0.05)。与阳性对照组比较,全方组、拆方1组、拆方2组、拆方3组无显著性差异(P>0.05)。全方组、拆方1组、拆方2组及拆方3组皮脂腺松散度组间比较无显著性差异(P>0.05)。
3.睾丸系数：全方组、拆方1组、拆方2组、拆方3组、阳性组睾丸系数与空白组比较无显著性差异(P>0.05)。
结论
1.痤疮灵全方及其各拆方组、阳性对照组(安体舒通)均能使皮脂腺斑面积缩小。
2.痤疮灵全方组、拆方2组均能使皮脂腺斑萎缩、变薄、腺叶松散。拆方1组、拆方3组及阳性组能够使皮脂腺腺叶松散,而腺叶变薄不明显。
3.痤疮灵与安体舒通疗效相当,整方疗效大于拆方各组；拆方各组中,抑制皮脂腺斑增生的疗效可能依次为：拆方2组>拆方1组疗>拆方3组。
4.痤疮灵全方及其拆方各组、阳性组均没有导致金黄地鼠睾丸萎缩的副作用。
由此可见,痤疮灵能够通过调节性激素水平抑制皮脂腺细胞的增生和分泌功能,影响皮脂腺斑的增生,有效缩小金黄地鼠侧腹部皮脂腺斑。其中起主要作用的药味是丹参、制大黄,其次为蒲公英、蛇舌草、葛根。
Objective:Acne vulgaris is a chronic imflammatory disorder of the pilosebaceous unit that occurs most commonly during adolescence,characterised by areas of skin with seborrheal,comedones(blackheads and whiteheads),papules, pustules, nodules and possible scarring. Acne affects mostly skin with the densest population of sebaceous follicles,these area include the face,the upper part of the chest and the back.Aside from scarring,acne negatively affects psychological health and psychosocial functioning,and even quality of life.Using the lateral abdominal sebaceous glands of a golden hamster as the experimental model,traditional Chinese medicine theory as a guide, by measuring the size and observing the microscopic structure of the sebaceous glands,the objective of the experiment is to expound the formula rationality of the traditional Chinese medicine(TCM) compound "Cuo Chuang Ling"pellet, and to find out effective drugs in the compound group,in order to further the research of its hormone influencing and mechanism in acne treating.
Methods:62male golden hamsters,weighing120-150g,giving normal breeding, randomly divided into six groups after a week:non-treatment control group has12golden hamsters,each of the whole prescriptions group, disassembled prescriptions group1,2, and3, positive control group has10golden hamsters.
At the beginning, the bilateral abdominal feather of the golden hamsters were completely cut with an electric shaver fully to expose the bilateral sebaceous glands. The maximum diameter (DT) and maximum longitudinal diameter (DL)of bilateral plaques were measured with caliper under the strong light. The each group of hamsters was fed the drug which was10times of human dosage.Animals of the whole prescriptions group were fed with11g/kg crude drug. Disassembled prescriptions group1,2, and3were given6g/kg,2g/kg,3g/kg crude drug separately.And the positive control group were10mg/kg dosage. Non-treatment control group was given an amount (1ml/100g) normal saline. Each group of golden hamsters wes fed once daily for4weeks. At the end of the experiment, the bilateral back hair of the golden hamsters were shaved again with an electric shaver, fully revealing the bilateral sebaceous glands.DT and DL of bilateral sebaceous glands were measured with caliper under the strong light.The tissue on the sebaceous glands approximately1.5cm*1.5cm were cut off after the hamsters were executed and were put in small bottles filled with10%formaldehyde liquid. Then the sebaceous glands were paraffined, sectioned, HE dyed,and the microstructual changes was being strutinized under a light-microscope. After the golden hamsters were dissected,their testicles were weighed and the testicular coefficient was calculated.
Results
1. The size of sebaceous glands:The size of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group is smaller compared with the sebaceous glands before treating,which has a very significant difference or significant difference (p<0.01or0.05)than before.However, the non-treatment control group has no significant difference (p>0.05). The size of the left sebaceous glands of whole prescriptions group, disassembled prescriptions group1,2and positive control group is much smaller compared with the non-treatment control group,which has a very significant difference (p<0.01),and disassembled prescriptions group3with a significant difference (p<0.05). The size of the right sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2become much smaller compared with the non-treatment control group which has a very significant difference (p<0.01),while disassembled prescriptions group3and positive control group with significant differences (p<0.05). The size of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant difference compared with the positive control group (p>0.05). The size of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).
2. Micro structural analysis of the sebaceous glands:The microstructure of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group loosen,becomes thinner, and the gland leaves are much smaller compared with the Blank group.Of them, whole prescriptions group, disassembled prescriptions2are obvious. Comparison of the thickness of the sebaceous glands:Thickness of the sebaceous glands of whole prescriptions group is much thinner than the non-treatment control group group.which has a very significant difference (p<0.01). Thickness of the sebaceous glands of disassembled prescriptions group2is thiner than the non-treatment control group which has a significant difference (P<0.05). Thickness of the sebaceous glands of disassembled prescriptions group1,3and the positive control group has no significant differences(P>0.05) compared with the non-treatment control group.Thickness of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant differences(p>0.05) compared with the positive control group.Thickness of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).Comparison of the loose degree of the sebaceous glands:The loose degree of the sebaceous glands of whole prescriptions group and disassembled prescriptions group1,2and positive control group is much looser compared with the non-treatment control group,which has a very significant difference(p<0.01), while disassembled prescriptions group3with a significant difference (P<0.05).The loose degree of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant difference (p>0.05) compared with the positive control group. The loose degree of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).
3. Testicular coefficient:The testicular coefficient of whole prescriptions group and its disassembled prescriptions groups, positive control group has no significant difference compared with the non-treatment control group (p>0.05).
Conclusion
1. The size of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups,positive (spironolactone) control group becomes smaller.
2. The microstructure of sebaceous glands of whole prescriptions group,disassembled prescriptions group2becomes atrophy, thinner, and the gland leaves loosen. The gland leaves of disassembled prescriptions group1,3and the positive control group loosen, while the gland leaves not become thinner obviously.
3. The whole prescriptions group of "Cuochuang ling" has equivalent effect with spironolactone in inhibiting hyperplasia of the sebaceous glands. The whole prescriptions group is more effective than its disassembled prescriptions groups. And the effect of inhibiting the hyperplasia of the sebaceous glands of the disassembled ones, disassembled prescriptions2group is best.next is the disassembled prescriptions group1,and the disassembled prescriptions group3is the last.
4. None of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group has side effects of inducing atrophy to the testicular of hamsters.
This shows proliferation and secrete function of the sebaceous glands can be inhibited,the sebaceous glands hyperplasia are influenced, and the golden hamsrer sebaceous glands are narrowed effectively by "Cuo Chuang Ling" through adjusting the sex hormone levels.Of the prescription,Danshen,processed Dahuang may play the main effct, followed by Pugongying,Baihuasheshecao and Gegen.
方法：雄性金黄地鼠62只,重120～150g。正常饲养一周后,随机分为六组,空白组12只,全方组、拆方1组、拆方2组、拆方3组、阳性对照组各10只。
实验开始时先用电动剃须刀剃尽金黄地鼠双侧背部毛,充分暴露皮脂腺斑,在强光照射下,用游标卡尺测量双侧斑块的最大横径(DT)和最大纵径(DL)。灌胃时,各组金黄地鼠均按人的10倍剂量给药。全方组按金黄地鼠11g/kg生药的剂量给药,拆方1组、拆方2组、拆方3组分别按金黄地鼠6g/kg、2g/kg、3g/kg生药的剂量给药；阳性对照组按10mg/kg剂量给药；空白组灌服等量(1ml/100g)生理盐水。每日一次,连续灌胃4周。实验结束时再次用电动剃须刀剃尽金黄地鼠背部毛,充分暴露双侧皮脂腺斑,在强光照射下,用游标卡尺测量双侧斑块的最大横径和最大纵径。处死动物后取下金黄地鼠侧背部皮脂腺斑组织,切取约1.5cm*1.5cm组织块,放入10%甲醛固定液小瓶中固定,常规石蜡包埋、切片、HE染色,于光镜下观察皮脂腺斑的组织学变化。解剖各组金黄地鼠并称取其睾丸重量,计算睾丸系数。
结果：
1.皮脂腺斑大小：痤疮灵全方组、拆方1组、拆方2组、拆方3组及阳性对照组皮脂腺斑较治疗前缩小,具有非常显著性差异或显著性差异(p<0.01或0.05),空白组皮脂腺斑与治疗前比较无显著性差异(p>0.05)；治疗后全方组、拆方1组、拆方2组及阳性对照组左侧皮脂腺斑面积较空白组明显缩小,具有非常显著性差异(p<0.01),拆方3组与空白组比较有显著性差异(p<0.05)；全方组、拆方1组、拆方2组右侧皮脂腺斑面积较空白组明显缩小,具有非常显著性差异(p<0.01),阳性对照组、拆方3组与空白组比较有显著性差异(p<0.05)。痤疮灵全方组、拆方1组、拆方2组、拆方3组皮脂腺斑大小与阳性对照组比较无显著性差异(p>0.05)；全方组、拆方1组、拆方2组、拆方3组皮脂腺斑大小组间比较无显著性差异(p>0.05)。
2.皮脂腺斑组织学结构：痤疮灵全方组、拆方1组、拆方2组、拆方3组及阳性对照组均较空白组皮脂腺松散,厚度变薄,皮脂腺腺叶变小；其中全方组、拆方2组较明显。皮脂腺厚度的比较：痤疮灵全方组皮脂腺厚度较空白组明显变薄,具有非常显著性差异(P<0.01)；拆方2组较空白组变薄,具有显著性差异(P<0.05)；拆方1组、拆方3组、阳性对照组与空白组比较,无显著性差异(P>0.05)。与阳性对照组比较,全方组、拆方1组、拆方2组、拆方3组均无显著性差异(P>0.05)。全方组、拆方1组、拆方2组及拆方3组皮脂腺厚度组间比较无显著性差异(P>0.05)。皮脂腺松散程度的比较：与空白组比较,痤疮灵全方组、拆方1组、拆方2组及阳性对照组均有非常显著性差异(P<0.01)；拆方3组具有显著性差异(P<0.05)。与阳性对照组比较,全方组、拆方1组、拆方2组、拆方3组无显著性差异(P>0.05)。全方组、拆方1组、拆方2组及拆方3组皮脂腺松散度组间比较无显著性差异(P>0.05)。
3.睾丸系数：全方组、拆方1组、拆方2组、拆方3组、阳性组睾丸系数与空白组比较无显著性差异(P>0.05)。
结论
1.痤疮灵全方及其各拆方组、阳性对照组(安体舒通)均能使皮脂腺斑面积缩小。
2.痤疮灵全方组、拆方2组均能使皮脂腺斑萎缩、变薄、腺叶松散。拆方1组、拆方3组及阳性组能够使皮脂腺腺叶松散,而腺叶变薄不明显。
3.痤疮灵与安体舒通疗效相当,整方疗效大于拆方各组；拆方各组中,抑制皮脂腺斑增生的疗效可能依次为：拆方2组>拆方1组疗>拆方3组。
4.痤疮灵全方及其拆方各组、阳性组均没有导致金黄地鼠睾丸萎缩的副作用。
由此可见,痤疮灵能够通过调节性激素水平抑制皮脂腺细胞的增生和分泌功能,影响皮脂腺斑的增生,有效缩小金黄地鼠侧腹部皮脂腺斑。其中起主要作用的药味是丹参、制大黄,其次为蒲公英、蛇舌草、葛根。
Objective:Acne vulgaris is a chronic imflammatory disorder of the pilosebaceous unit that occurs most commonly during adolescence,characterised by areas of skin with seborrheal,comedones(blackheads and whiteheads),papules, pustules, nodules and possible scarring. Acne affects mostly skin with the densest population of sebaceous follicles,these area include the face,the upper part of the chest and the back.Aside from scarring,acne negatively affects psychological health and psychosocial functioning,and even quality of life.Using the lateral abdominal sebaceous glands of a golden hamster as the experimental model,traditional Chinese medicine theory as a guide, by measuring the size and observing the microscopic structure of the sebaceous glands,the objective of the experiment is to expound the formula rationality of the traditional Chinese medicine(TCM) compound "Cuo Chuang Ling"pellet, and to find out effective drugs in the compound group,in order to further the research of its hormone influencing and mechanism in acne treating.
Methods:62male golden hamsters,weighing120-150g,giving normal breeding, randomly divided into six groups after a week:non-treatment control group has12golden hamsters,each of the whole prescriptions group, disassembled prescriptions group1,2, and3, positive control group has10golden hamsters.
At the beginning, the bilateral abdominal feather of the golden hamsters were completely cut with an electric shaver fully to expose the bilateral sebaceous glands. The maximum diameter (DT) and maximum longitudinal diameter (DL)of bilateral plaques were measured with caliper under the strong light. The each group of hamsters was fed the drug which was10times of human dosage.Animals of the whole prescriptions group were fed with11g/kg crude drug. Disassembled prescriptions group1,2, and3were given6g/kg,2g/kg,3g/kg crude drug separately.And the positive control group were10mg/kg dosage. Non-treatment control group was given an amount (1ml/100g) normal saline. Each group of golden hamsters wes fed once daily for4weeks. At the end of the experiment, the bilateral back hair of the golden hamsters were shaved again with an electric shaver, fully revealing the bilateral sebaceous glands.DT and DL of bilateral sebaceous glands were measured with caliper under the strong light.The tissue on the sebaceous glands approximately1.5cm*1.5cm were cut off after the hamsters were executed and were put in small bottles filled with10%formaldehyde liquid. Then the sebaceous glands were paraffined, sectioned, HE dyed,and the microstructual changes was being strutinized under a light-microscope. After the golden hamsters were dissected,their testicles were weighed and the testicular coefficient was calculated.
Results
1. The size of sebaceous glands:The size of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group is smaller compared with the sebaceous glands before treating,which has a very significant difference or significant difference (p<0.01or0.05)than before.However, the non-treatment control group has no significant difference (p>0.05). The size of the left sebaceous glands of whole prescriptions group, disassembled prescriptions group1,2and positive control group is much smaller compared with the non-treatment control group,which has a very significant difference (p<0.01),and disassembled prescriptions group3with a significant difference (p<0.05). The size of the right sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2become much smaller compared with the non-treatment control group which has a very significant difference (p<0.01),while disassembled prescriptions group3and positive control group with significant differences (p<0.05). The size of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant difference compared with the positive control group (p>0.05). The size of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).
2. Micro structural analysis of the sebaceous glands:The microstructure of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group loosen,becomes thinner, and the gland leaves are much smaller compared with the Blank group.Of them, whole prescriptions group, disassembled prescriptions2are obvious. Comparison of the thickness of the sebaceous glands:Thickness of the sebaceous glands of whole prescriptions group is much thinner than the non-treatment control group group.which has a very significant difference (p<0.01). Thickness of the sebaceous glands of disassembled prescriptions group2is thiner than the non-treatment control group which has a significant difference (P<0.05). Thickness of the sebaceous glands of disassembled prescriptions group1,3and the positive control group has no significant differences(P>0.05) compared with the non-treatment control group.Thickness of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant differences(p>0.05) compared with the positive control group.Thickness of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).Comparison of the loose degree of the sebaceous glands:The loose degree of the sebaceous glands of whole prescriptions group and disassembled prescriptions group1,2and positive control group is much looser compared with the non-treatment control group,which has a very significant difference(p<0.01), while disassembled prescriptions group3with a significant difference (P<0.05).The loose degree of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant difference (p>0.05) compared with the positive control group. The loose degree of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).
3. Testicular coefficient:The testicular coefficient of whole prescriptions group and its disassembled prescriptions groups, positive control group has no significant difference compared with the non-treatment control group (p>0.05).
Conclusion
1. The size of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups,positive (spironolactone) control group becomes smaller.
2. The microstructure of sebaceous glands of whole prescriptions group,disassembled prescriptions group2becomes atrophy, thinner, and the gland leaves loosen. The gland leaves of disassembled prescriptions group1,3and the positive control group loosen, while the gland leaves not become thinner obviously.
3. The whole prescriptions group of "Cuochuang ling" has equivalent effect with spironolactone in inhibiting hyperplasia of the sebaceous glands. The whole prescriptions group is more effective than its disassembled prescriptions groups. And the effect of inhibiting the hyperplasia of the sebaceous glands of the disassembled ones, disassembled prescriptions2group is best.next is the disassembled prescriptions group1,and the disassembled prescriptions group3is the last.
4. None of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group has side effects of inducing atrophy to the testicular of hamsters.
This shows proliferation and secrete function of the sebaceous glands can be inhibited,the sebaceous glands hyperplasia are influenced, and the golden hamsrer sebaceous glands are narrowed effectively by "Cuo Chuang Ling" through adjusting the sex hormone levels.Of the prescription,Danshen,processed Dahuang may play the main effct, followed by Pugongying,Baihuasheshecao and Gegen.
引文
[1]Jeong Eun Do, M.D.,Sun-Mi Cho, Ph.D., Sung-I1 In, M.D.et al. Psychosocial Aspects of Acne Vulgaris:A Community-based Study with Korean Adolescents. Ann Dermatol,2009,21 (2):125-126.
[2]Jon A Halvorsen, Florence Dalgard, Magne Thoresen.et al. Is the association between acne and mental distress influenced by diet? Results from a cross-sectional population study among 3775 late adolescents in Oslo, Norway. BMC Public Health,2009,9:340.
[3]S. Zahra Ghodsi 1, Helmut Orawa, Christos C. Zouboulis. Prevalence, Severity, and Severity Risk Factors of Acne in High School Pupils:A Community-Based Study. Journal of Investigative Dermatology,2009, 129:2136.
[4]Yiwei Shen, Tinglin Wang, Cheng Zhou. Prevalence of Acne Vulgaris in Chinese Adolescents and Adults:A Community-based Study of 17,345 Subjects in Six Cities[R]. Acta Derm Venereol 2012,92: 40-44.
[5]冯洁莹,范瑞强.广州市1561名中学生痤疮流行病学调查[J].岭南皮肤性病科杂志,2008,15(1)：49-51.
[6]庞莹.痤疮的流行病学及与雄激素受体基因多态性的相关研究[D].中国医科大学,2008.
[7]王大光,朱文元.痤疮发病中毛囊皮脂腺导管异常角化的机制[J].中国麻风皮肤病杂志,2005,21(1)
[8]赵辨.中国临床皮肤病学[M].第四版.南京：江苏科学技术出版社,2010.1165.
[9]中国医师协会皮肤科医师分会《中国痤疮治疗指南》专家组.中国痤疮治疗指南(讨论稿)[J].临床皮肤科杂志,2008,37(5)：339
[10]吴小红,刘瓦利.细胞免疫和体液免疫在寻常性痤疮发病中的作用[J].中国麻风皮肤病杂志,2006,22(11)：915-916.
[1]]林新瑜,罗旭松,董巍,等.痤疮患者血清白介素-1α、白介素-6、白介素-8、α-肿瘤坏死因子水平的检测分析[J].临床皮肤科杂志,2003,32(6)：335-336.
[12]Wen-Chieh Chen, Christos C.Zouboulis.Hormones and the pilosebaceous unit[J].Dermato-Endocrinology, 2009,1 (2):81-86.
[13]鞠强,沈丹蓓,夏隆庆.人皮脂腺细胞与雄激素关系的研究进展.国外医学皮肤性病学分册,2002,28(6)：372-374.
[14]鞠强,夏隆庆.皮脂腺与痤疮[J].中国医学科学院学报,2007,,4：272-274.
[15]Zouboulis CC,Seltmann H,Hiroi N,et al.Corticotropin releasing hormone:an autocrine hormone that promotes lipogenesis in human sebocytes[J].Proc Natl Acad Sci USA,2002,99(10):7148-7153.
[16]郭红卫,郝飞.皮肤HPA轴的研究进展[J].中国皮肤性病学杂志,2009,23(12)：841-843.
[17]吴巧云,郑敏.皮脂腺功能及调控的研究进展[J].医学综述,2006,.12(20)：1217-1219.
[18]Ignacio Vega-Naredo,Cristina Tomas-Zapico,Ana Coto-Montes. Potential role of autophagy in behavioral changes of the flank organ[J]. Autophagy,2009,5(2):265.
[19]刘文彬.王晖.痤疮模型的研究现状[J].广东药学院学报,2010,26(2)：212.
[20]GomezEC. Hamster flank organ:assessment of drugs modulating sebaceous gland function.In Maibach H, eds.Models in Dermatology.Basel:Ka rger,1985,100-111.
[21]程玮,翟所迪.痤疮的药物治疗评价[J].中国医院用药评价与分析,2008,8(11).
[22]Dreno B, Bettoli V, Ochsendorf F, et al. European recommendations on the use of oral antibiotics for acne[J]. Eur J Dermatol,2004,14(6):391-399.
[23]Katsambas AD, Stefanaki C, Cunliffe WJ. Guidelines for treating acne[J]. Clin Dermatol,2004,22(5): 439-444.
[24]Sinclair W, Jordaan HF, Global Alliance to Improve Outcomes in Acne. Acne guideline 2005 update[J]. S Afr Med J,2005,95:881-892.
[25]金艳霞,孙虹.痤疮的中西医治疗现状[J].皮肤病与性病,2008.30(4)：27.
[26]王强.异维A酸治疗中、重度痤疮的疗效观察[J].上海医药,2009,30(9)
[27]王昌媛,张福仁.光与寻常性痤疮的治疗.国外医学皮肤性病学分册,2004,30(6)：348-350.
[28]穆艳蕾,杨蓉娅.物理疗法应用于痤疮治疗[J].感染、炎症、修复,2006,7(3)
[29]游明芝,王远清.LED红蓝光治疗痤疮145例临床疗效观察[J].中国医药指南,2011,9(22)：275-276.
[30]樊昕,刘丽红,岳丹霞,等.5-氨基酮戊酸光动力疗法治疗中重度痤疮的临床观察[J].中国美容医学,2010,19(3)：363-365.
[31]邢月贞摘译,杨蓉娅校.585nm脉冲激光在治疗痤疮中的作用及其与其他局部疗法的比较[J].实用皮肤病学杂志,2010,3(2)：108.
[32]吴春华.皮肤病名家验案精选[M].第1版.北京：人民军医出版社,2007.161.
[33]高新彦,马宽玉.古今名医皮肤性病科医案赏析[M].第1版.北京：人民军医出版社,2006.298.
[34]李艳萍,张友堂.痤疮病因病机探讨[J].中国中医基础医学杂志,2004,10(11)：59-60..
[35]李敏,李红毅.范瑞强教授治疗女性痤疮经验简介[J].中国中西医结合皮肤性病学杂志,2009,8(3)160-161.
[36]中国中医研究院广安门医院.朱仁康临床经验集——皮肤外科[M].第1版.北京：人民卫生出版社,2005.204-206.
[37]管汾.实用中医皮肤病学[M].第1版.兰州：甘肃人民出版社,1981.211-212.
[38]顾伯康.中医外科学[M].第1版.上海：上海科学技术出版社,1986.151.
[39]郑春玉,李兴广.李兴广治疗痤疮临床经验[J].中国中医基础医学杂志,201],17(3)：341-342.
[40]涂明.程桂英教授治疗痤疮经验[J].中医药导报,2011,17(2)：7-8.
[41]李慧林.中医辨证治疗痤疮的体会[J].新中医,2011,43(1)：132
[42]郑定辉,杨娴.消痤汤治疗女性痤疮的临床研究[J].中国美容医学,2008,17(12)：1791-1792.
[43]胡艳君,雪彦锋,姚守恩等.清肺利湿解毒方药治疗痤疮的临床观察及对血清相关性激素水平的影响[J].中国皮肤性病学杂志,2010,24(6)：568-569.
[44]熊德,马丽昕,陈绿娥.养阴清痤冲剂与螺内酯治疗女性持久性痤疮对比研究[J].实用中医药杂志,2008,24(9)：551-552.
[45]李智珍,池凤好,范瑞强.滋阴清肝消痤方配合痤灵酊治疗成年女性阴虚肝热型痤疮临床疗效观察[J].广州中医药大学学报,2007,24(1)：30-32.
[46]刘俐,唐春蕾,隋丽华等.消毒饮治疗重度痤疮疗效观察[J].辽宁中医杂志,2008,35(5)：735.
[47]徐萍,陈力.疏肝滋肾法治疗女性寻常性痤疮的临床疗效观察[J],中国美容医学,2010,19(])：106-107.
[48]杨柳.颠倒散加味治疗寻常痤疮342例[J].中医外治杂志,2001,10(5)：34.
[49]朱红军.中药消痤霜外治面部寻常痤疮198例疗效观察[J].四川中医,2009,27(12)：101-102.
[50]王醊恩,林莺,杨毅.痤消膏外治痤疮54例疗效观察[J].长春中医药大学学报,2010,26(2)：244.
[51]易建平,黄联继,易建昌等.粉刺灵凝胶外治青年寻常痤疮128例疗效观察[J].新中医,2003,35(4)：23-24.
[52]张理梅.中西医结合治疗顽固性痤疮经验及体会[J].现代中医药,2010,30(3)：3.
[53]韩平,戴永江,辜孔进等.桑丹消痤净面膜治疗寻常性痤疮43例临床观察[J].中国中医药科技,2009,16(5)：410-411.
[54]戚合德,周勇.龙胆消痤方对寻常型痤疮临床疗效及皮脂分泌的影响[J].中国临床医生,2010,38(5)：47-48.
[55]周展超,郑家润,徐文严.外用2.5%丹参酮霜治疗寻常痤疮的临床和实验研究[J].临床皮肤科杂志,1996(6)
[56]吕全兴.丹参等中药治疗寻常痤疮机理的实验研究[D].山东中医药大学硕士学位论文,2006.
[57]杨岚,瞿幸.痤疮合剂治疗寻常痤疮肺胃湿热证的临床和实验研究[J].中华中医药杂志,2010,35(3)：470-472.
[58]张友堂,尹霞,孙丽英.痤疮颗粒治疗寻常痤疮的临床与实验研究[J].中医药信息,2007,24(2)58-59.
[59]刘靖,邝枣园,陈妙欢.滋阴清肝消座汤治疗寻常座疮48例临床与实验研究[J].中医杂志,2005,46(8)：605-607.
[60]何遥,杜玲.清热散结汤治疗痤疮的机理探讨[J].海南医学,2010,21(22)：99.
[61]于中蛟,李保强,薛承岩等.丹参酮对女性迟发性痤疮患者血清性激素水平的影响及疗效观察[J].河北医药,2009,31(2)：149-150.
[62]周展超,郑家润,徐文严.安体舒通及丹参酮抗金黄地鼠皮脂腺斑增生的研究[J].中华皮肤科杂志,1997,30(3)：182-183.
[63]汪五清,杜锡贤.中药痤疮饮性激素样活性的实验研究[J].福建中医药,2006,37(2)：43-44.
[64]陈力,徐萍,郭顺.痤疮灵颗粒对金黄地鼠皮脂腺斑组织形态的影响[J].南京中医药大学学报,2011,26(6)：459-460.
[65]鞠强,尹兴平,石继海,等.丹参酮对人皮脂腺细胞增殖&脂质合成及雄性激素受体mRNA表达的影响[J].中华皮肤科杂志,2005,38(2)：98-100.
[66]兰东,司天润,赵树玲等.清热养阴、理气活血法治疗女性迟发性与持久性痤疮的临床与实验室研究[J].中国中西医结合皮肤性病学杂志,2004,3(1)：11-14.
[67]张志勇,刘慧,杨巧利.粉刺消颗粒治疗痤疮的临床疗效及对血清IL-6,IL-8水平的影响[J].实用临床医药杂志,2011.15(19)：63-65.
[68]李果,罗云,肖小河等.六味地黄汤及其拆方的配伍规律实验研究[J].中药材,2007,30(2)：205-206.
[69]高晶,曾勇,于飞等.痹祺胶囊全方及拆方抗炎镇痛作用研究[J].中草药,2009,40(])：93-96.
[70]倪新强,韩新民.泻肺平喘灵及其拆方对“肺合大肠”模型大鼠血清内毒素及肿瘤坏死因子-α的影响[J].2011,17(14)：137-138.
[71]崔翰博,韩涛,张东钰等.补阳还五汤及拆方对代谢综合征模型大鼠胰岛素、TXA2水平的影响[J].山东中医药大学学报,2011,35(5)：446-447.
[72]韩凌,彭燕,赵瑞芝等.银屑灵全方及拆方对角质形成细胞HaCaT增殖的影响[J].广州中医药大学学报,2011,28(2)：159-162.
[73]胡锐,冯康,杨瑞瑞等.正交试验设计孔圣枕中丹抗小鼠抑郁作用的拆方研究[J].中药药理与临床,2010,26(5)：19-21.
[74]沈鸿,黄芳,窦昌贵.酸枣仁汤处方的配比研究[J].中国药科大学学报,2002,33,(1)：21-23.
[75]徐长化,孙江桥,李波等.定喘汤及其拆方的药理作用[J].中国医院药学杂志,2002,22(4)：202-204.
[76]吴忠祥,贺龙刚,周丽等.半夏泻心汤及其拆方对Hp感染小鼠胃黏膜保护作用的研究[J].湖南中医药大学学报,2010,30(5)：23-25.
[77]丁杨,朱萱萱,郁晓维.运脾温阳颗粒及拆方含药血清对正常和脾虚小鼠脾淋巴细胞增殖的影响[J].实用中医内科杂志,2008,22(5)：65-66.
[78]王停,韩玲,荆鲁.中药、天然药物复方口服制剂临床前拆方研究中的问题[J].中国中药杂志,2006,31(15)：1299.
[79]周斌,刘可越,常军.麻杏石甘汤的拆方和抗平喘之间相关性研究[J].时珍国医国药,2011,22(1)：136-137.
[80]徐维佳,周海虹,陈少东.均匀设计在中药复方研究中的应用分析[J].中国实验方剂学杂志,2010,16(13)：236.
[81]高艳青,刘晓霓,高蔚等.半夏泻心汤及其类方不同配伍对正常大鼠胃酸分泌的影响[J].数理医药学杂志,2004,17(2)：149-152.
[82]李广清.痹痛灵拆方的实验研究[D].南京中医药大学,2005.
[83]龙旭阳,郑攀.方剂拆方研究现状[J].浙江中医药大学学报,2010,34(1)：120-121.
[84]刘晴,施建蓉.中药复方拆方研究[J].中西医结合学报,2003,1(3)：173-176.
[85]李国华,席小平,贾继峰Wistar大鼠主要脏器系数与体重相关性的探讨[J].山西医药杂志,2005,34(8)：661-662.
[86]Monica Ottaviani, Emanuela Camera, Mauro Picardo.LipidMediators in Acne. Mediators of Inflammation,2010.
[87]陈秋江.安体舒通联合维A酸霜治疗痤疮的临床疗效观察[J].中外医疗,2011(3)：108.
[88]郝国娈,来文华,高宜云.咪唑斯汀联合安体舒通治疗女性迟发性痤疮的疗效[J].实用医学杂志, 2010,26(23)：4391-4392.
[89]鲁祖清,杨闰平,徐辉.米诺环素胶囊联合螺内酯治疗痤疮的疗效观察[J].中国临床医生,2011,39(8)：58-59.
[90]高学敏.中药学[M].第一版.北京：中国中医药出版社,2002.129,158,580.
[91]孙长霞,童应凯,黄亮等。蒲公英不同溶剂提取物体外抑菌效果的研究[J].食品科技,2012,37(1)：204-206.
[92]吴小红,刘瓦利,于勇.蛇丹方复方及其构成生药对痤疮丙酸杆菌的抑制作用[J].中国中西医结合皮肤性病学,2003,2(4)：222-224.
[93]李国旺,苗志国,赵恒章.大黄的体外抑菌实验[J].光谱实验室,2011,28(1)：66-68.
[94]张骏,翁福海,李会强等.大黄素对大鼠腹腔巨噬细胞产生的TNFA、IL-1、IL-6及细胞[Ca2+]i的影响[J].中草药,2001,32(8)：718-721.
[95]刘昌,刘原兴,吕毅.大黄素抑制体外淋巴细胞增殖的作用研究[J]第四军医大学学报,2006,27(24)：2251-2252.
[96]李林,瞿小婷,陆阳.植物雌激素的筛选及抗皮肤老化的体外研究[J].上海交通大学学报(医学版),2011,31(10)：1361-1365
[97]林岷格,韩平,戴永江,等.丹参酮对寻常性痤疮患者血清睾酮、雌二醇的影响[J].山东医药,2009,49(48)：58-59.
[98]高玉桂,王灵芝,唐翼雪..丹参酮的性激素样活性[J].中国医学科学院学报,1980,2(3)：189-191.
[99]赵丕文,牛建昭,王继峰.6种中药活性成分植物雌激素作用的比较研究[J].中国药学杂志,2007,42(24)：1852-1855.
[100]程科军.Ⅰ覆盆子活性成分研究Ⅱ金雀根中二苯乙烯类成分的稳定性研究[D].复旦大学,2008.
[101]陈力,王晨,徐萍.痤疮灵颗粒治疗寻常痤疮的临床研究.南京中医药大学学报,2009,25(6)：467-469.
[102]Katsuhiro Iinuma, Takashi Sato, Noriko Akimoto.et al. Involvement of Propionibacterium acnes in the Augmentation of Lipogenesis in Hamster Sebaceous Glands In Vivo and In Vitro[J]. The Society for Investigative Dermatology,2009:2113-2118.
[2]Jon A Halvorsen, Florence Dalgard, Magne Thoresen.et al. Is the association between acne and mental distress influenced by diet? Results from a cross-sectional population study among 3775 late adolescents in Oslo, Norway. BMC Public Health,2009,9:340.
[3]S. Zahra Ghodsi 1, Helmut Orawa, Christos C. Zouboulis. Prevalence, Severity, and Severity Risk Factors of Acne in High School Pupils:A Community-Based Study. Journal of Investigative Dermatology,2009, 129:2136.
[4]Yiwei Shen, Tinglin Wang, Cheng Zhou. Prevalence of Acne Vulgaris in Chinese Adolescents and Adults:A Community-based Study of 17,345 Subjects in Six Cities[R]. Acta Derm Venereol 2012,92: 40-44.
[5]冯洁莹,范瑞强.广州市1561名中学生痤疮流行病学调查[J].岭南皮肤性病科杂志,2008,15(1)：49-51.
[6]庞莹.痤疮的流行病学及与雄激素受体基因多态性的相关研究[D].中国医科大学,2008.
[7]王大光,朱文元.痤疮发病中毛囊皮脂腺导管异常角化的机制[J].中国麻风皮肤病杂志,2005,21(1)
[8]赵辨.中国临床皮肤病学[M].第四版.南京：江苏科学技术出版社,2010.1165.
[9]中国医师协会皮肤科医师分会《中国痤疮治疗指南》专家组.中国痤疮治疗指南(讨论稿)[J].临床皮肤科杂志,2008,37(5)：339
[10]吴小红,刘瓦利.细胞免疫和体液免疫在寻常性痤疮发病中的作用[J].中国麻风皮肤病杂志,2006,22(11)：915-916.
[1]]林新瑜,罗旭松,董巍,等.痤疮患者血清白介素-1α、白介素-6、白介素-8、α-肿瘤坏死因子水平的检测分析[J].临床皮肤科杂志,2003,32(6)：335-336.
[12]Wen-Chieh Chen, Christos C.Zouboulis.Hormones and the pilosebaceous unit[J].Dermato-Endocrinology, 2009,1 (2):81-86.
[13]鞠强,沈丹蓓,夏隆庆.人皮脂腺细胞与雄激素关系的研究进展.国外医学皮肤性病学分册,2002,28(6)：372-374.
[14]鞠强,夏隆庆.皮脂腺与痤疮[J].中国医学科学院学报,2007,,4：272-274.
[15]Zouboulis CC,Seltmann H,Hiroi N,et al.Corticotropin releasing hormone:an autocrine hormone that promotes lipogenesis in human sebocytes[J].Proc Natl Acad Sci USA,2002,99(10):7148-7153.
[16]郭红卫,郝飞.皮肤HPA轴的研究进展[J].中国皮肤性病学杂志,2009,23(12)：841-843.
[17]吴巧云,郑敏.皮脂腺功能及调控的研究进展[J].医学综述,2006,.12(20)：1217-1219.
[18]Ignacio Vega-Naredo,Cristina Tomas-Zapico,Ana Coto-Montes. Potential role of autophagy in behavioral changes of the flank organ[J]. Autophagy,2009,5(2):265.
[19]刘文彬.王晖.痤疮模型的研究现状[J].广东药学院学报,2010,26(2)：212.
[20]GomezEC. Hamster flank organ:assessment of drugs modulating sebaceous gland function.In Maibach H, eds.Models in Dermatology.Basel:Ka rger,1985,100-111.
[21]程玮,翟所迪.痤疮的药物治疗评价[J].中国医院用药评价与分析,2008,8(11).
[22]Dreno B, Bettoli V, Ochsendorf F, et al. European recommendations on the use of oral antibiotics for acne[J]. Eur J Dermatol,2004,14(6):391-399.
[23]Katsambas AD, Stefanaki C, Cunliffe WJ. Guidelines for treating acne[J]. Clin Dermatol,2004,22(5): 439-444.
[24]Sinclair W, Jordaan HF, Global Alliance to Improve Outcomes in Acne. Acne guideline 2005 update[J]. S Afr Med J,2005,95:881-892.
[25]金艳霞,孙虹.痤疮的中西医治疗现状[J].皮肤病与性病,2008.30(4)：27.
[26]王强.异维A酸治疗中、重度痤疮的疗效观察[J].上海医药,2009,30(9)
[27]王昌媛,张福仁.光与寻常性痤疮的治疗.国外医学皮肤性病学分册,2004,30(6)：348-350.
[28]穆艳蕾,杨蓉娅.物理疗法应用于痤疮治疗[J].感染、炎症、修复,2006,7(3)
[29]游明芝,王远清.LED红蓝光治疗痤疮145例临床疗效观察[J].中国医药指南,2011,9(22)：275-276.
[30]樊昕,刘丽红,岳丹霞,等.5-氨基酮戊酸光动力疗法治疗中重度痤疮的临床观察[J].中国美容医学,2010,19(3)：363-365.
[31]邢月贞摘译,杨蓉娅校.585nm脉冲激光在治疗痤疮中的作用及其与其他局部疗法的比较[J].实用皮肤病学杂志,2010,3(2)：108.
[32]吴春华.皮肤病名家验案精选[M].第1版.北京：人民军医出版社,2007.161.
[33]高新彦,马宽玉.古今名医皮肤性病科医案赏析[M].第1版.北京：人民军医出版社,2006.298.
[34]李艳萍,张友堂.痤疮病因病机探讨[J].中国中医基础医学杂志,2004,10(11)：59-60..
[35]李敏,李红毅.范瑞强教授治疗女性痤疮经验简介[J].中国中西医结合皮肤性病学杂志,2009,8(3)160-161.
[36]中国中医研究院广安门医院.朱仁康临床经验集——皮肤外科[M].第1版.北京：人民卫生出版社,2005.204-206.
[37]管汾.实用中医皮肤病学[M].第1版.兰州：甘肃人民出版社,1981.211-212.
[38]顾伯康.中医外科学[M].第1版.上海：上海科学技术出版社,1986.151.
[39]郑春玉,李兴广.李兴广治疗痤疮临床经验[J].中国中医基础医学杂志,201],17(3)：341-342.
[40]涂明.程桂英教授治疗痤疮经验[J].中医药导报,2011,17(2)：7-8.
[41]李慧林.中医辨证治疗痤疮的体会[J].新中医,2011,43(1)：132
[42]郑定辉,杨娴.消痤汤治疗女性痤疮的临床研究[J].中国美容医学,2008,17(12)：1791-1792.
[43]胡艳君,雪彦锋,姚守恩等.清肺利湿解毒方药治疗痤疮的临床观察及对血清相关性激素水平的影响[J].中国皮肤性病学杂志,2010,24(6)：568-569.
[44]熊德,马丽昕,陈绿娥.养阴清痤冲剂与螺内酯治疗女性持久性痤疮对比研究[J].实用中医药杂志,2008,24(9)：551-552.
[45]李智珍,池凤好,范瑞强.滋阴清肝消痤方配合痤灵酊治疗成年女性阴虚肝热型痤疮临床疗效观察[J].广州中医药大学学报,2007,24(1)：30-32.
[46]刘俐,唐春蕾,隋丽华等.消毒饮治疗重度痤疮疗效观察[J].辽宁中医杂志,2008,35(5)：735.
[47]徐萍,陈力.疏肝滋肾法治疗女性寻常性痤疮的临床疗效观察[J],中国美容医学,2010,19(])：106-107.
[48]杨柳.颠倒散加味治疗寻常痤疮342例[J].中医外治杂志,2001,10(5)：34.
[49]朱红军.中药消痤霜外治面部寻常痤疮198例疗效观察[J].四川中医,2009,27(12)：101-102.
[50]王醊恩,林莺,杨毅.痤消膏外治痤疮54例疗效观察[J].长春中医药大学学报,2010,26(2)：244.
[51]易建平,黄联继,易建昌等.粉刺灵凝胶外治青年寻常痤疮128例疗效观察[J].新中医,2003,35(4)：23-24.
[52]张理梅.中西医结合治疗顽固性痤疮经验及体会[J].现代中医药,2010,30(3)：3.
[53]韩平,戴永江,辜孔进等.桑丹消痤净面膜治疗寻常性痤疮43例临床观察[J].中国中医药科技,2009,16(5)：410-411.
[54]戚合德,周勇.龙胆消痤方对寻常型痤疮临床疗效及皮脂分泌的影响[J].中国临床医生,2010,38(5)：47-48.
[55]周展超,郑家润,徐文严.外用2.5%丹参酮霜治疗寻常痤疮的临床和实验研究[J].临床皮肤科杂志,1996(6)
[56]吕全兴.丹参等中药治疗寻常痤疮机理的实验研究[D].山东中医药大学硕士学位论文,2006.
[57]杨岚,瞿幸.痤疮合剂治疗寻常痤疮肺胃湿热证的临床和实验研究[J].中华中医药杂志,2010,35(3)：470-472.
[58]张友堂,尹霞,孙丽英.痤疮颗粒治疗寻常痤疮的临床与实验研究[J].中医药信息,2007,24(2)58-59.
[59]刘靖,邝枣园,陈妙欢.滋阴清肝消座汤治疗寻常座疮48例临床与实验研究[J].中医杂志,2005,46(8)：605-607.
[60]何遥,杜玲.清热散结汤治疗痤疮的机理探讨[J].海南医学,2010,21(22)：99.
[61]于中蛟,李保强,薛承岩等.丹参酮对女性迟发性痤疮患者血清性激素水平的影响及疗效观察[J].河北医药,2009,31(2)：149-150.
[62]周展超,郑家润,徐文严.安体舒通及丹参酮抗金黄地鼠皮脂腺斑增生的研究[J].中华皮肤科杂志,1997,30(3)：182-183.
[63]汪五清,杜锡贤.中药痤疮饮性激素样活性的实验研究[J].福建中医药,2006,37(2)：43-44.
[64]陈力,徐萍,郭顺.痤疮灵颗粒对金黄地鼠皮脂腺斑组织形态的影响[J].南京中医药大学学报,2011,26(6)：459-460.
[65]鞠强,尹兴平,石继海,等.丹参酮对人皮脂腺细胞增殖&脂质合成及雄性激素受体mRNA表达的影响[J].中华皮肤科杂志,2005,38(2)：98-100.
[66]兰东,司天润,赵树玲等.清热养阴、理气活血法治疗女性迟发性与持久性痤疮的临床与实验室研究[J].中国中西医结合皮肤性病学杂志,2004,3(1)：11-14.
[67]张志勇,刘慧,杨巧利.粉刺消颗粒治疗痤疮的临床疗效及对血清IL-6,IL-8水平的影响[J].实用临床医药杂志,2011.15(19)：63-65.
[68]李果,罗云,肖小河等.六味地黄汤及其拆方的配伍规律实验研究[J].中药材,2007,30(2)：205-206.
[69]高晶,曾勇,于飞等.痹祺胶囊全方及拆方抗炎镇痛作用研究[J].中草药,2009,40(])：93-96.
[70]倪新强,韩新民.泻肺平喘灵及其拆方对“肺合大肠”模型大鼠血清内毒素及肿瘤坏死因子-α的影响[J].2011,17(14)：137-138.
[71]崔翰博,韩涛,张东钰等.补阳还五汤及拆方对代谢综合征模型大鼠胰岛素、TXA2水平的影响[J].山东中医药大学学报,2011,35(5)：446-447.
[72]韩凌,彭燕,赵瑞芝等.银屑灵全方及拆方对角质形成细胞HaCaT增殖的影响[J].广州中医药大学学报,2011,28(2)：159-162.
[73]胡锐,冯康,杨瑞瑞等.正交试验设计孔圣枕中丹抗小鼠抑郁作用的拆方研究[J].中药药理与临床,2010,26(5)：19-21.
[74]沈鸿,黄芳,窦昌贵.酸枣仁汤处方的配比研究[J].中国药科大学学报,2002,33,(1)：21-23.
[75]徐长化,孙江桥,李波等.定喘汤及其拆方的药理作用[J].中国医院药学杂志,2002,22(4)：202-204.
[76]吴忠祥,贺龙刚,周丽等.半夏泻心汤及其拆方对Hp感染小鼠胃黏膜保护作用的研究[J].湖南中医药大学学报,2010,30(5)：23-25.
[77]丁杨,朱萱萱,郁晓维.运脾温阳颗粒及拆方含药血清对正常和脾虚小鼠脾淋巴细胞增殖的影响[J].实用中医内科杂志,2008,22(5)：65-66.
[78]王停,韩玲,荆鲁.中药、天然药物复方口服制剂临床前拆方研究中的问题[J].中国中药杂志,2006,31(15)：1299.
[79]周斌,刘可越,常军.麻杏石甘汤的拆方和抗平喘之间相关性研究[J].时珍国医国药,2011,22(1)：136-137.
[80]徐维佳,周海虹,陈少东.均匀设计在中药复方研究中的应用分析[J].中国实验方剂学杂志,2010,16(13)：236.
[81]高艳青,刘晓霓,高蔚等.半夏泻心汤及其类方不同配伍对正常大鼠胃酸分泌的影响[J].数理医药学杂志,2004,17(2)：149-152.
[82]李广清.痹痛灵拆方的实验研究[D].南京中医药大学,2005.
[83]龙旭阳,郑攀.方剂拆方研究现状[J].浙江中医药大学学报,2010,34(1)：120-121.
[84]刘晴,施建蓉.中药复方拆方研究[J].中西医结合学报,2003,1(3)：173-176.
[85]李国华,席小平,贾继峰Wistar大鼠主要脏器系数与体重相关性的探讨[J].山西医药杂志,2005,34(8)：661-662.
[86]Monica Ottaviani, Emanuela Camera, Mauro Picardo.LipidMediators in Acne. Mediators of Inflammation,2010.
[87]陈秋江.安体舒通联合维A酸霜治疗痤疮的临床疗效观察[J].中外医疗,2011(3)：108.
[88]郝国娈,来文华,高宜云.咪唑斯汀联合安体舒通治疗女性迟发性痤疮的疗效[J].实用医学杂志, 2010,26(23)：4391-4392.
[89]鲁祖清,杨闰平,徐辉.米诺环素胶囊联合螺内酯治疗痤疮的疗效观察[J].中国临床医生,2011,39(8)：58-59.
[90]高学敏.中药学[M].第一版.北京：中国中医药出版社,2002.129,158,580.
[91]孙长霞,童应凯,黄亮等。蒲公英不同溶剂提取物体外抑菌效果的研究[J].食品科技,2012,37(1)：204-206.
[92]吴小红,刘瓦利,于勇.蛇丹方复方及其构成生药对痤疮丙酸杆菌的抑制作用[J].中国中西医结合皮肤性病学,2003,2(4)：222-224.
[93]李国旺,苗志国,赵恒章.大黄的体外抑菌实验[J].光谱实验室,2011,28(1)：66-68.
[94]张骏,翁福海,李会强等.大黄素对大鼠腹腔巨噬细胞产生的TNFA、IL-1、IL-6及细胞[Ca2+]i的影响[J].中草药,2001,32(8)：718-721.
[95]刘昌,刘原兴,吕毅.大黄素抑制体外淋巴细胞增殖的作用研究[J]第四军医大学学报,2006,27(24)：2251-2252.
[96]李林,瞿小婷,陆阳.植物雌激素的筛选及抗皮肤老化的体外研究[J].上海交通大学学报(医学版),2011,31(10)：1361-1365
[97]林岷格,韩平,戴永江,等.丹参酮对寻常性痤疮患者血清睾酮、雌二醇的影响[J].山东医药,2009,49(48)：58-59.
[98]高玉桂,王灵芝,唐翼雪..丹参酮的性激素样活性[J].中国医学科学院学报,1980,2(3)：189-191.
[99]赵丕文,牛建昭,王继峰.6种中药活性成分植物雌激素作用的比较研究[J].中国药学杂志,2007,42(24)：1852-1855.
[100]程科军.Ⅰ覆盆子活性成分研究Ⅱ金雀根中二苯乙烯类成分的稳定性研究[D].复旦大学,2008.
[101]陈力,王晨,徐萍.痤疮灵颗粒治疗寻常痤疮的临床研究.南京中医药大学学报,2009,25(6)：467-469.
[102]Katsuhiro Iinuma, Takashi Sato, Noriko Akimoto.et al. Involvement of Propionibacterium acnes in the Augmentation of Lipogenesis in Hamster Sebaceous Glands In Vivo and In Vitro[J]. The Society for Investigative Dermatology,2009:2113-2118.