平胃散治疗湿困脾胃证大鼠的作用机制初探
|
摘要
目的在传统中医学理论指导下,运用现代研究手段,以前期实验中成功创建的湿困脾胃证动物模型为实验平台,探讨平胃散治疗湿困脾胃证的作用机制。方法本文回顾了湿困脾胃证及平胃散的形成、发展、研究状况等,为本课题的研究提供了思路及奠定了理论基础。在此理论基础之上,以导师所创建湿困脾胃证动物模型为平台,通过间苯三酚法、放射免疫、酶联免疫法、硝酸还原酶法等检测平胃散对模型大鼠肠道组织血清D-木糖、胃肠激素P物质(SP)、血管活性肠肽(VIF)及神经递质(一氧化氮NO)的干预情况;通过定磷法检测脑组织Na~+-K~+-ATP酶活性,观测平胃散对模型大鼠脑组织钠泵活性影响情况。
结果
1、模型组大鼠血清D-木糖含量与空白对照组相比明显下降,经平胃散治疗后显著回升,治疗组与自然恢复组无统计学意义,但趋势好于自然恢复组;模型组大鼠肠道组织内SP含量比空白对照组显著上升,经平胃散治疗后明显下降,治疗组与自然恢复组无统计学意义,但趋势好于自然恢复组;模型大鼠肠道组织NO含量与空白对照组比较显著上升,治疗组比模型组含量明显下降,治疗组与自然恢复组无统计学意义,但趋势好于自然恢复组;模型组大鼠肠道组织VIP含量与空白对照组无统计学意义。
2、模型组脑组织Na~+-K~+-ATP酶活性比空白对照组明显降低,经平胃散治疗后该酶活性显著提高,同时自然恢复组该酶活性没有恢复,与平胃散组有明显差异。
结论
1、实验中发现湿困脾胃证大鼠胃肠功能下降、动力紊乱和胃肠激素分泌异常;平胃散对该证大鼠胃肠功能和动力的改善机制可能为调节肠神经系统(ENS)中某些相关胃肠激素(胃泌素、胃动素MTL、P物质SP)和神经递质(NO)的释放。
2、钠泵活性的降低是湿困脾胃证的复杂发病机制之一,平胃散可有效提高湿困脾胃所导致的Na~+-K~+-ATP酶活性降低,这是平胃散多层次、多靶点治疗该证的其中一项重要环节。
Objective: This experiment drew support from in the earlier period experiment tosucceed the establishment experimental animal model platform. With the guide of the theory of TCM, to discuss the Therapeutic Mechanism of the pingwei powder treats dampness obstructing spleen-stomach syndrome by modern research method.
Methods: This article reviewed the research status of dampness obstructingspleen-stomach syndrome and origin and development of pingwei powder, which providing a theoretical foundation for the experimental study. The experiment use the method of phloroglucinol to test the D-xylose of serum, radioimmunoassay and Enzyme-linked immenosorbent assay to test the intervention situation of pingwei powder to gastrointestinal hormone and the neurotransmitter, phosphorus measurement to test Na~+-K~+-ATP enzyme of brain tissue.
Results:
1. There was obvious decrease of D-xylose of serum of Model rats compare to rats of blank control group. There was no significant difference in D-xylose of serum between pingwei powder group and spontaneous recovery group, but general tendency of pingwei powder group much better than spontaneous recovery group. There was obvious increase of Substance P in intestinal tissue of Model rats compare to rats of blank control group. After the treatment by pingwei powder, no significant difference from Substance P in intestinal tissue between pingwei powder group and spontaneous recovery group. But in pingwei powder group, it was a downtrend. General tendency of pingwei powder group much better than spontaneous recovery group. There was obvious increase of Nitric Oxide in intestinal tissue of Model rats compare to rats of blank control group, and obvious decrease in rats of pingwei powder groups compare to rats of modern rats. Meanwhile, there isn't significant difference between pingwei powder group and spontaneous recovery group. There is no significant difference from VIP in intestinal tissue between modern rats and blank control group.
2. There was obvious decrease of Na~+-K~+-ATP enzyme in brain tissue of Model rats compare to rats of blank control group, and obvious increase in rats of pingwei powder groups compare to modern rats. Meanwhile, there is significant difference between pingwei powder group and spontaneous recovery group.
Conclusion:
1. Model rats of dampness obstructing spleen-stomach syndrome to be found gastrointestinal function deceased, gastric motility disorders and abnormal gastrointestinal hormone secretion in the earlier period experiment, Pingwei powder can intensify the gastrointestinal function and regulate gastrointestinal motility by modifying interrelated gastrointestinal hormone and neuro transmitters release in enteric nervous system.
2. The activities of natrium pump be the one of the complicated pathogenesis, pingwei powder can enhance the activity of the Na~+-K~+-ATP enzyme of the model rats. It suggests that above-mentioned results be likely to some of multi - hierarchy and multi-targets therapeutic mechanism.
结果
1、模型组大鼠血清D-木糖含量与空白对照组相比明显下降,经平胃散治疗后显著回升,治疗组与自然恢复组无统计学意义,但趋势好于自然恢复组;模型组大鼠肠道组织内SP含量比空白对照组显著上升,经平胃散治疗后明显下降,治疗组与自然恢复组无统计学意义,但趋势好于自然恢复组;模型大鼠肠道组织NO含量与空白对照组比较显著上升,治疗组比模型组含量明显下降,治疗组与自然恢复组无统计学意义,但趋势好于自然恢复组;模型组大鼠肠道组织VIP含量与空白对照组无统计学意义。
2、模型组脑组织Na~+-K~+-ATP酶活性比空白对照组明显降低,经平胃散治疗后该酶活性显著提高,同时自然恢复组该酶活性没有恢复,与平胃散组有明显差异。
结论
1、实验中发现湿困脾胃证大鼠胃肠功能下降、动力紊乱和胃肠激素分泌异常;平胃散对该证大鼠胃肠功能和动力的改善机制可能为调节肠神经系统(ENS)中某些相关胃肠激素(胃泌素、胃动素MTL、P物质SP)和神经递质(NO)的释放。
2、钠泵活性的降低是湿困脾胃证的复杂发病机制之一,平胃散可有效提高湿困脾胃所导致的Na~+-K~+-ATP酶活性降低,这是平胃散多层次、多靶点治疗该证的其中一项重要环节。
Objective: This experiment drew support from in the earlier period experiment tosucceed the establishment experimental animal model platform. With the guide of the theory of TCM, to discuss the Therapeutic Mechanism of the pingwei powder treats dampness obstructing spleen-stomach syndrome by modern research method.
Methods: This article reviewed the research status of dampness obstructingspleen-stomach syndrome and origin and development of pingwei powder, which providing a theoretical foundation for the experimental study. The experiment use the method of phloroglucinol to test the D-xylose of serum, radioimmunoassay and Enzyme-linked immenosorbent assay to test the intervention situation of pingwei powder to gastrointestinal hormone and the neurotransmitter, phosphorus measurement to test Na~+-K~+-ATP enzyme of brain tissue.
Results:
1. There was obvious decrease of D-xylose of serum of Model rats compare to rats of blank control group. There was no significant difference in D-xylose of serum between pingwei powder group and spontaneous recovery group, but general tendency of pingwei powder group much better than spontaneous recovery group. There was obvious increase of Substance P in intestinal tissue of Model rats compare to rats of blank control group. After the treatment by pingwei powder, no significant difference from Substance P in intestinal tissue between pingwei powder group and spontaneous recovery group. But in pingwei powder group, it was a downtrend. General tendency of pingwei powder group much better than spontaneous recovery group. There was obvious increase of Nitric Oxide in intestinal tissue of Model rats compare to rats of blank control group, and obvious decrease in rats of pingwei powder groups compare to rats of modern rats. Meanwhile, there isn't significant difference between pingwei powder group and spontaneous recovery group. There is no significant difference from VIP in intestinal tissue between modern rats and blank control group.
2. There was obvious decrease of Na~+-K~+-ATP enzyme in brain tissue of Model rats compare to rats of blank control group, and obvious increase in rats of pingwei powder groups compare to modern rats. Meanwhile, there is significant difference between pingwei powder group and spontaneous recovery group.
Conclusion:
1. Model rats of dampness obstructing spleen-stomach syndrome to be found gastrointestinal function deceased, gastric motility disorders and abnormal gastrointestinal hormone secretion in the earlier period experiment, Pingwei powder can intensify the gastrointestinal function and regulate gastrointestinal motility by modifying interrelated gastrointestinal hormone and neuro transmitters release in enteric nervous system.
2. The activities of natrium pump be the one of the complicated pathogenesis, pingwei powder can enhance the activity of the Na~+-K~+-ATP enzyme of the model rats. It suggests that above-mentioned results be likely to some of multi - hierarchy and multi-targets therapeutic mechanism.
引文
[1]郭金龙,颜正华.湿阻证病理造型的实验研究[J].中医杂志1988,(8):39-41
[2]张六通,梅家俊,黄志红,等.外湿致病机理的实验研究[J].中医杂志,1999,40(8):496-497
[3]姜学连.湿邪致病机理的初步实验研究[J].宾州医学院学报,1992,15(4):306-308
[4]陈孝银,劳永生,严灿,等.湿困脾胃证大鼠胃肠功能的改变及健脾祛湿方药的影响[J].四川中医,1999,17(1):4-5
[5]黄秀深,赵燕,等.中焦湿阻证大鼠红细胞膜Na~+-K~+ATP酶活性研究[J].成都中医药大学学报.2000,23(4):23-24.
[6]黄秀深,刘德芳,陈洁,等.大鼠中焦湿阻证与抗利尿激素关系的实验研究[J].成都中医药大学学报.2002,25(1):35-36
[7]严灿,高敏,等.湿阻证患者调节功能的临床观察及其机理探讨[J].中国中医基础医学杂志,1998,4(6):41-43
[8]刘德芳,黄秀深,胡鹏,等.湿阻证大鼠红细胞免疫功能变化及平胃散对它的影响[J].深圳中西结合杂志.2002,12(3):141-143
[9]王学清,等.平胃散对大鼠胃排空影响的实验研究[J].世界华人消化杂志,2002,10(6):719-720
[10]刘德芳,黄秀深,胡鹏,等[J].湿阻证大鼠红细胞免疫功能变化及平胃散对它的影响.深圳中西结合杂志.2002,12(3):141-143
[11]肖桦,黄秀深,张丰华,等.湿阻中焦大鼠红细胞内外电解质浓度变化的实验研究[J].成都中医药大学学报2004,27(3):25-26
[12]黄秀深,赵燕.中焦湿阻证大鼠红细胞膜Na~+-K~+-ATP酶活性研究.成都中医药大学学报[J].2000,23(4):23-24
[13]刘德芳,黄秀深,张丰华,等.大鼠湿阻中焦证的水盐代谢调节机制及平胃散对其的影响[J].中国中医药信息杂志 2005,12(1)26-27
[14]黄秀深,张丰华.平胃散对湿阻中焦模型大鼠血浆抗利尿激素及红细胞内钠、钾浓度的影响.[J]中医杂志.2003,(6):456-458
[15]黄秀深,贾波,张丰华.平胃散拆方研究对湿阻模型ALD,ANF,NT的结论分析[J].湖南中医学院学报.2004,24:140-142
[16]Kunt T,Forst T,Schmidt S,et al.serum levels of substance P are decreased in patients with type Ⅰ diabetes.[J]Exp Clin Endocrinol Diabetes,2000,108:164-167
[17]陈永萍,李任先,等.肠易激综合征不同中医证型的神经内分泌学研究[J].第一军医大学学报,2001,21(3):202-204
[18]贾波,邓中甲,黄秀深,等.白术茯苓汤及其配伍对脾虚大鼠胃肠激素的影响.[J]成都中医药大学学报,2002,25(2):13-15
[19]王斌,严灿.湿阻证患者免疫功能调节改变的临床研究.[J]新中医.2005,37(8):15-16.
[20]刘伟,黄秀深,罗玉熙.湿阻证动物模型研究进展.[J]四川中医.2005,24(4):35-36
[21]KimuraH,HokariR,MiuraS,etal.Increased expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitdte in colonic mucosa of patients with active ulcerative colitis.[J]Gut,1998,42:180-7
[22]Sanders KM,Ward SM.Nitric oxide as a mediator nonadrennergic nocholinergic neurotransmission.[J]AMJ.Physiol.1992,262:379-392
[23]吴丽丽,陈吉力.自由基损伤与湿邪致病机理的探讨[J].贵阳中医学院学报,2000,22(4):1-2
[24]罗玉熙,黄秀深,张瑞,等.平胃散对湿困脾胃证大鼠肠道P物质及NO含量的调控.高洁等主编.方剂的现代研究[M].中医古籍出版社,2006:111-114
[25]黄秀深,罗玉熙,沈涛,等.胃苓汤调节湿困脾胃证大鼠脑组织Na~+.K~+-ATP酶活性的实验研究.高洁等主编.方剂的现代研究[M].中医古籍出版社,2006:151-154
[26]周礼卿.脾虚患者小肠吸收、胰外分泌功能的同步观察[J].广州中医药大学学报,1986,3(4):41
[27]郑芝田.胃肠病学[M].第2版.北京:人民卫生出版社,1993.1201-1207.
[28]许国铭,李石.现代消化病学[M].第1版.北京:人民军医出版社,1997.16-27.
[29]杨文友,等.平胃散加味治疗慢性肾功能不全氮质血症的研究[J].中国中医药信息杂志,1998,5(5):33
[30]王美霞.平胃散的临床新用[J].陕西中医,1998,19(1):35
[31]张茹,王福贤.肠易激综合征患者血浆及乙状结肠粘膜中VIP及SS的含量[J].标记免疫分析与临床,2004,11(2):81-84
[32]李兆申,董文珠,邹多武,等.肠易激综合征肠黏膜SP、VIP、CGRP变化的研究[J].第二军医大学学报,2003,24(2):147-151
[33]赵梦云,娄桂贤,陈继龙,等.肠易激综合症患者回结肠黏膜VIP活性与结肠动力的关系研究[J].基础医学与临床,2003,23(增刊):91
[34]赵智强,项小人,汪受传,等.儿宝颗粒对厌食症大鼠模型血中5-HT及空肠段SP、VIP、SS表达的影响[J].中国中西医结合消化杂志,2006,14(2):96-99
[35]李非,孙家邦,刘爽,等.实验性胆道梗阻时大鼠胃运动和胃肠肽类激素的变化[J].中华普通外科杂志,2000,15(3):165-169
[36]计敏,林琳,赵志泉,等.糖尿病动物模型血浆和肠道组织中P物质含量的变化及其意义[J].基础医学与临床,2001:21增刊:78
[37]杜立峰,展淑琴,郭新奎,等.P物质、血管活性肠肽与异常胃肠通过的关系[J].西安交通大学学报(医学版)2003,24(4):363
[38]刘渡舟.湿证论[J].北京中医药大学学报,1998,21(1):3-9
[39]李克华,邹宝生,胡玲,等.湿阻中焦的辨证施治[J].陕西中医,2001,22(10):640
[40]陈元方主编.胃肠激素基础与临床[M].北匕京:中国协和医科大学出版社,1997.
[41]周吕,柯美云.胃肠动力学[M].第一版,北京:中国科学技术出版社,1999:29-40,51-60
[42]Fukuo K.Enhancement f NO bioavailability by ACEI and ARB[J].NIPPon Rinsho,2004,62(9):561-566.
[43]姚永莉,宋于刚,张万岱,等.胃肠道功能紊乱与胃动力关系的实验研究川[J].第一军医大学学报,2003,27,(8):33.
[44]彭成.试论中医证候动物模型的研究技术[J].成都中医学院学报,1989,12(3):44
[45]吴建红.简述平胃散源流发展[J].湖南中医学院学报,1996,1(2):29-30
[46]彭怀仁主编.中医方剂大辞典卷三[M].北京:人民卫生出版社,1996:28
[1]刘伟,黄秀深,罗玉熙.湿阻证动物模型研究进展.四川中医.2005,24(4):35-36
[2]KimuraH,HokariR,MiuraS,etal.Increased expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitrite in colonic mucosa of patients with active ulcerative colitis.Gut,1998,42:180-7[
3]Sanders KM,Ward SM.Nitric oxide as a mediator nonadrennergic nocholinergic neurotransmission.AMJ.Physiol.1992,262:379-392
[4]郭金龙,颜正华.湿阻证病理造型的实验研究[J].中医杂志 1988,(8):39-41
[5]姜学连.湿邪致病机理的初步实验研究[J].宾州医学院学报,1992,15(4):306-308
[6]吴丽丽,陈吉力.自由基损伤与湿邪致病机理的探讨[J].贵阳中医学院学报,2000,22(4):1-2
[7]张六通,梅家俊,黄志红等.外湿致病机理的实验研究[J].中医杂志,1999,40(8):496-497
[8]陈孝银,劳永生,严灿,等.湿困脾胃证大鼠胃肠功能的改变及健脾祛湿方药的影响[J].四川中医,1999,17(1):4-5
[2]张六通,梅家俊,黄志红,等.外湿致病机理的实验研究[J].中医杂志,1999,40(8):496-497
[3]姜学连.湿邪致病机理的初步实验研究[J].宾州医学院学报,1992,15(4):306-308
[4]陈孝银,劳永生,严灿,等.湿困脾胃证大鼠胃肠功能的改变及健脾祛湿方药的影响[J].四川中医,1999,17(1):4-5
[5]黄秀深,赵燕,等.中焦湿阻证大鼠红细胞膜Na~+-K~+ATP酶活性研究[J].成都中医药大学学报.2000,23(4):23-24.
[6]黄秀深,刘德芳,陈洁,等.大鼠中焦湿阻证与抗利尿激素关系的实验研究[J].成都中医药大学学报.2002,25(1):35-36
[7]严灿,高敏,等.湿阻证患者调节功能的临床观察及其机理探讨[J].中国中医基础医学杂志,1998,4(6):41-43
[8]刘德芳,黄秀深,胡鹏,等.湿阻证大鼠红细胞免疫功能变化及平胃散对它的影响[J].深圳中西结合杂志.2002,12(3):141-143
[9]王学清,等.平胃散对大鼠胃排空影响的实验研究[J].世界华人消化杂志,2002,10(6):719-720
[10]刘德芳,黄秀深,胡鹏,等[J].湿阻证大鼠红细胞免疫功能变化及平胃散对它的影响.深圳中西结合杂志.2002,12(3):141-143
[11]肖桦,黄秀深,张丰华,等.湿阻中焦大鼠红细胞内外电解质浓度变化的实验研究[J].成都中医药大学学报2004,27(3):25-26
[12]黄秀深,赵燕.中焦湿阻证大鼠红细胞膜Na~+-K~+-ATP酶活性研究.成都中医药大学学报[J].2000,23(4):23-24
[13]刘德芳,黄秀深,张丰华,等.大鼠湿阻中焦证的水盐代谢调节机制及平胃散对其的影响[J].中国中医药信息杂志 2005,12(1)26-27
[14]黄秀深,张丰华.平胃散对湿阻中焦模型大鼠血浆抗利尿激素及红细胞内钠、钾浓度的影响.[J]中医杂志.2003,(6):456-458
[15]黄秀深,贾波,张丰华.平胃散拆方研究对湿阻模型ALD,ANF,NT的结论分析[J].湖南中医学院学报.2004,24:140-142
[16]Kunt T,Forst T,Schmidt S,et al.serum levels of substance P are decreased in patients with type Ⅰ diabetes.[J]Exp Clin Endocrinol Diabetes,2000,108:164-167
[17]陈永萍,李任先,等.肠易激综合征不同中医证型的神经内分泌学研究[J].第一军医大学学报,2001,21(3):202-204
[18]贾波,邓中甲,黄秀深,等.白术茯苓汤及其配伍对脾虚大鼠胃肠激素的影响.[J]成都中医药大学学报,2002,25(2):13-15
[19]王斌,严灿.湿阻证患者免疫功能调节改变的临床研究.[J]新中医.2005,37(8):15-16.
[20]刘伟,黄秀深,罗玉熙.湿阻证动物模型研究进展.[J]四川中医.2005,24(4):35-36
[21]KimuraH,HokariR,MiuraS,etal.Increased expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitdte in colonic mucosa of patients with active ulcerative colitis.[J]Gut,1998,42:180-7
[22]Sanders KM,Ward SM.Nitric oxide as a mediator nonadrennergic nocholinergic neurotransmission.[J]AMJ.Physiol.1992,262:379-392
[23]吴丽丽,陈吉力.自由基损伤与湿邪致病机理的探讨[J].贵阳中医学院学报,2000,22(4):1-2
[24]罗玉熙,黄秀深,张瑞,等.平胃散对湿困脾胃证大鼠肠道P物质及NO含量的调控.高洁等主编.方剂的现代研究[M].中医古籍出版社,2006:111-114
[25]黄秀深,罗玉熙,沈涛,等.胃苓汤调节湿困脾胃证大鼠脑组织Na~+.K~+-ATP酶活性的实验研究.高洁等主编.方剂的现代研究[M].中医古籍出版社,2006:151-154
[26]周礼卿.脾虚患者小肠吸收、胰外分泌功能的同步观察[J].广州中医药大学学报,1986,3(4):41
[27]郑芝田.胃肠病学[M].第2版.北京:人民卫生出版社,1993.1201-1207.
[28]许国铭,李石.现代消化病学[M].第1版.北京:人民军医出版社,1997.16-27.
[29]杨文友,等.平胃散加味治疗慢性肾功能不全氮质血症的研究[J].中国中医药信息杂志,1998,5(5):33
[30]王美霞.平胃散的临床新用[J].陕西中医,1998,19(1):35
[31]张茹,王福贤.肠易激综合征患者血浆及乙状结肠粘膜中VIP及SS的含量[J].标记免疫分析与临床,2004,11(2):81-84
[32]李兆申,董文珠,邹多武,等.肠易激综合征肠黏膜SP、VIP、CGRP变化的研究[J].第二军医大学学报,2003,24(2):147-151
[33]赵梦云,娄桂贤,陈继龙,等.肠易激综合症患者回结肠黏膜VIP活性与结肠动力的关系研究[J].基础医学与临床,2003,23(增刊):91
[34]赵智强,项小人,汪受传,等.儿宝颗粒对厌食症大鼠模型血中5-HT及空肠段SP、VIP、SS表达的影响[J].中国中西医结合消化杂志,2006,14(2):96-99
[35]李非,孙家邦,刘爽,等.实验性胆道梗阻时大鼠胃运动和胃肠肽类激素的变化[J].中华普通外科杂志,2000,15(3):165-169
[36]计敏,林琳,赵志泉,等.糖尿病动物模型血浆和肠道组织中P物质含量的变化及其意义[J].基础医学与临床,2001:21增刊:78
[37]杜立峰,展淑琴,郭新奎,等.P物质、血管活性肠肽与异常胃肠通过的关系[J].西安交通大学学报(医学版)2003,24(4):363
[38]刘渡舟.湿证论[J].北京中医药大学学报,1998,21(1):3-9
[39]李克华,邹宝生,胡玲,等.湿阻中焦的辨证施治[J].陕西中医,2001,22(10):640
[40]陈元方主编.胃肠激素基础与临床[M].北匕京:中国协和医科大学出版社,1997.
[41]周吕,柯美云.胃肠动力学[M].第一版,北京:中国科学技术出版社,1999:29-40,51-60
[42]Fukuo K.Enhancement f NO bioavailability by ACEI and ARB[J].NIPPon Rinsho,2004,62(9):561-566.
[43]姚永莉,宋于刚,张万岱,等.胃肠道功能紊乱与胃动力关系的实验研究川[J].第一军医大学学报,2003,27,(8):33.
[44]彭成.试论中医证候动物模型的研究技术[J].成都中医学院学报,1989,12(3):44
[45]吴建红.简述平胃散源流发展[J].湖南中医学院学报,1996,1(2):29-30
[46]彭怀仁主编.中医方剂大辞典卷三[M].北京:人民卫生出版社,1996:28
[1]刘伟,黄秀深,罗玉熙.湿阻证动物模型研究进展.四川中医.2005,24(4):35-36
[2]KimuraH,HokariR,MiuraS,etal.Increased expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitrite in colonic mucosa of patients with active ulcerative colitis.Gut,1998,42:180-7[
3]Sanders KM,Ward SM.Nitric oxide as a mediator nonadrennergic nocholinergic neurotransmission.AMJ.Physiol.1992,262:379-392
[4]郭金龙,颜正华.湿阻证病理造型的实验研究[J].中医杂志 1988,(8):39-41
[5]姜学连.湿邪致病机理的初步实验研究[J].宾州医学院学报,1992,15(4):306-308
[6]吴丽丽,陈吉力.自由基损伤与湿邪致病机理的探讨[J].贵阳中医学院学报,2000,22(4):1-2
[7]张六通,梅家俊,黄志红等.外湿致病机理的实验研究[J].中医杂志,1999,40(8):496-497
[8]陈孝银,劳永生,严灿,等.湿困脾胃证大鼠胃肠功能的改变及健脾祛湿方药的影响[J].四川中医,1999,17(1):4-5