基于穴位脏腑相关性的足三里穴对胃肠功能紊乱大鼠的调节作用及机制研究
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摘要
目的:通过观察电针足三里穴对正常及胃肠功能紊乱大鼠的调节作用探讨足三里穴与胃特异性的效应机制。
方法:80只SD大鼠随机分为正常和模型两部分,腹腔注射利血平制作胃肠功能紊乱模型,两种方法电针足三里、三阴交穴,共10组,每组8只。激光多普勒微循环血流分析仪监测胃、肠、肝、脑等器官表面微循环。多道生理记录仪监测胃肠生物电。高效液相、放免法检测血液及组织中5-HT、DA、NE、E、MTL、GAS、ET等神经递质、胃肠激素含量。免疫组化法检测5-HT、VIP在胃、肠、脑组织中的表达。原位杂交法检测VIPR_2 mRNA在胃、肠、脑组织中的表达。
结果:对于正常大鼠,电针能升高胃、肠微循环,足三里2组尤为显著(P＜0.05);能使胃肠生物电振幅降低,频率增快,足三里组胃电频率较三阴交组明显增快,有显著性差异(P＜0.05);血浆ET含量降低(P＜0.05),升高血及组织中MTL、GAS含量(P＜0.05),血清及下丘脑中NE、E、DA含量升高,但对5-HT影响不明显;胃、肠、脑组织中的5-HT、VIP、VIPR_2 mRNA表达升高(P＜0.05)。
对于模型大鼠,电针能升高降低的胃、肠微循环,较三阴交穴,足三里穴升高胃微循环更明显(P＜0.05);能使胃肠生物电振幅降低,频率增快;血浆ET含量降低(P＜0.05),升高血及组织中MTL含量,升高血、脑GAS含量,降低胃GAS含量,可升高E、DA含量,升高下丘脑5-HT含量;降低血清5-HT含量及组织中的表达(P＜0.05),可升高模型大鼠降低的VIP及其受体的表达(P＜0.05)。
结论:电针足三里穴能有效调节正常及胃肠功能紊乱模型大鼠胃肠微循环、生物电及神经递质、胃肠激素等相关指标的变化,具有一定的穴位特异性,提示足三里穴与胃之间存在特异性关系,为穴位脏腑相关性理论提供一定科学依据。
Objective:To explore the special interation between Zusanli and stomach through observing the regulating effect and mechanism of electroacupuncture(EA) at Zusanli in normal and model rats with functional gastrointestinal disorders(FGID).
Methods:80 SD rats were randomly divided into normal and model,included 10 groups with 6 cases in each according to the difference of Zusanli and Sanyinjiao.FGID model was established by intraperitoneal injection of reserpine.EA was carried on Zusanli and Sanyinjiao.The microcirculatory blood flow was observed with a laser doppler blood flow monitor in the surface of stomach,duodenum,liver and brain,and the bioelectrical feature of stomach and intestine detected with multi track polygraph.The neurotransmitter and gut hormone in the blood and tissues such as 5-HT,DA,NE,E,MTL,GAS and ET were detected with the methods of radioimmunity and high performance liquid chromatography.Methods of immunohistochemistry and in situ hybridization were used to detect the expression of 5-HT,VIP and VIPR_2 mRNA.
Results:For the normal rats,the microcirculatory of stomach and intestine was improved by EA,especially Zusanli 2 group(P＜0.05).The amplitude was decreased,while the frequency of bioelectricity was increased,Zusanli is more significant than Sanyinjiao at the frequency(P＜0.05).The content of ET was reduced by EA(P＜0.05),the content of MTL,GAS was enhanced,the content of NE,E and DA was raised by EA,while 5-HT was not significant.The expression of 5-HT,VIP,VIPR_2 mRNA in normal stomach was enhanced in stomach,intestine and brain(P＜0.05).For the model rats,the descendent microcirculatory of stomach and intestine was improved by EA,and Zusanli was more apparente than Sanyinjiao in the microcirculatory of stomach.The amplitude was decreased,while the frequency of bioelectricity was increased,The ascendant content of ET was reduced(P＜0.05),the content of MTL was enhanced,the serum and intestine content of GAS was increased,the stomach's was reduced,the content of E,DA,5-HT in hypothalamus was raised,the serum content of 5-HT was reduced by EA.The ascendent expression of 5-HT was depressed,and the decendant expression of VIP,VIPR_2 mRNA was enhanced in stomach,intestine and brain of the model rats by EA(P＜0.05).
Conclusion:Microcirculation,bioelectricity and related biochemical indicators of the normal and model rats with FGID can be effectively regulated by EA at Zusanli.That may be verified that Zusanli is a certain specific point to stomach and has special relations between them,which provided scientific evidence for the theory of acupoint and visceral coupling.
方法:80只SD大鼠随机分为正常和模型两部分,腹腔注射利血平制作胃肠功能紊乱模型,两种方法电针足三里、三阴交穴,共10组,每组8只。激光多普勒微循环血流分析仪监测胃、肠、肝、脑等器官表面微循环。多道生理记录仪监测胃肠生物电。高效液相、放免法检测血液及组织中5-HT、DA、NE、E、MTL、GAS、ET等神经递质、胃肠激素含量。免疫组化法检测5-HT、VIP在胃、肠、脑组织中的表达。原位杂交法检测VIPR_2 mRNA在胃、肠、脑组织中的表达。
结果:对于正常大鼠,电针能升高胃、肠微循环,足三里2组尤为显著(P＜0.05);能使胃肠生物电振幅降低,频率增快,足三里组胃电频率较三阴交组明显增快,有显著性差异(P＜0.05);血浆ET含量降低(P＜0.05),升高血及组织中MTL、GAS含量(P＜0.05),血清及下丘脑中NE、E、DA含量升高,但对5-HT影响不明显;胃、肠、脑组织中的5-HT、VIP、VIPR_2 mRNA表达升高(P＜0.05)。
对于模型大鼠,电针能升高降低的胃、肠微循环,较三阴交穴,足三里穴升高胃微循环更明显(P＜0.05);能使胃肠生物电振幅降低,频率增快;血浆ET含量降低(P＜0.05),升高血及组织中MTL含量,升高血、脑GAS含量,降低胃GAS含量,可升高E、DA含量,升高下丘脑5-HT含量;降低血清5-HT含量及组织中的表达(P＜0.05),可升高模型大鼠降低的VIP及其受体的表达(P＜0.05)。
结论:电针足三里穴能有效调节正常及胃肠功能紊乱模型大鼠胃肠微循环、生物电及神经递质、胃肠激素等相关指标的变化,具有一定的穴位特异性,提示足三里穴与胃之间存在特异性关系,为穴位脏腑相关性理论提供一定科学依据。
Objective:To explore the special interation between Zusanli and stomach through observing the regulating effect and mechanism of electroacupuncture(EA) at Zusanli in normal and model rats with functional gastrointestinal disorders(FGID).
Methods:80 SD rats were randomly divided into normal and model,included 10 groups with 6 cases in each according to the difference of Zusanli and Sanyinjiao.FGID model was established by intraperitoneal injection of reserpine.EA was carried on Zusanli and Sanyinjiao.The microcirculatory blood flow was observed with a laser doppler blood flow monitor in the surface of stomach,duodenum,liver and brain,and the bioelectrical feature of stomach and intestine detected with multi track polygraph.The neurotransmitter and gut hormone in the blood and tissues such as 5-HT,DA,NE,E,MTL,GAS and ET were detected with the methods of radioimmunity and high performance liquid chromatography.Methods of immunohistochemistry and in situ hybridization were used to detect the expression of 5-HT,VIP and VIPR_2 mRNA.
Results:For the normal rats,the microcirculatory of stomach and intestine was improved by EA,especially Zusanli 2 group(P＜0.05).The amplitude was decreased,while the frequency of bioelectricity was increased,Zusanli is more significant than Sanyinjiao at the frequency(P＜0.05).The content of ET was reduced by EA(P＜0.05),the content of MTL,GAS was enhanced,the content of NE,E and DA was raised by EA,while 5-HT was not significant.The expression of 5-HT,VIP,VIPR_2 mRNA in normal stomach was enhanced in stomach,intestine and brain(P＜0.05).For the model rats,the descendent microcirculatory of stomach and intestine was improved by EA,and Zusanli was more apparente than Sanyinjiao in the microcirculatory of stomach.The amplitude was decreased,while the frequency of bioelectricity was increased,The ascendant content of ET was reduced(P＜0.05),the content of MTL was enhanced,the serum and intestine content of GAS was increased,the stomach's was reduced,the content of E,DA,5-HT in hypothalamus was raised,the serum content of 5-HT was reduced by EA.The ascendent expression of 5-HT was depressed,and the decendant expression of VIP,VIPR_2 mRNA was enhanced in stomach,intestine and brain of the model rats by EA(P＜0.05).
Conclusion:Microcirculation,bioelectricity and related biochemical indicators of the normal and model rats with FGID can be effectively regulated by EA at Zusanli.That may be verified that Zusanli is a certain specific point to stomach and has special relations between them,which provided scientific evidence for the theory of acupoint and visceral coupling.
引文
[1]Rong PJ,Li L.Relationship between internal organ(viscera) and acupoints:toward meridian theory.World J Acu-moxi.2008;18(2):44-50.
[2]林亚平,易受乡,严洁,等.电针足阳明经穴对胃粘膜损伤大鼠胃粘膜血流量及生长抑素的影响.中国中西医结合消化杂志,2003;11(2):75-77.
[3]张泓,易受乡,严洁,等.电针足三阳经穴对家兔脑肠肽类物质影响的比较研究.中国临床康复.2004,12:2290-2291.
[4]英振吴.电针足三里穴对实验性胃肠道功能紊乱大鼠模型的影响及调理作用的研究.2007硕士学位论文全文数据库.
[5]邓春雷.实验针灸学.北京:人民卫生出版社,1998,第1版:7.
[6]姬广臣,史仁华,赵鲁鸣.电针对完全结扎双侧颈总动脉后大鼠软脑膜微循环血流量的影响.中国针灸,1997;17(10):606.
[7]连学建,王建武,郭莲军,等.胃克星对大鼠肠系膜微循环的影响.临床消化病杂志,2003;15(6):265-266.
[8]张丽,顾瑛,刘凡光,等.光动力作用对大鼠肠系膜微循环的影响.中国激光医学杂志,2003;5(12):69-75.
[9]赵保民,黄裕新,赵宁侠,等.电针调控大鼠胃酸分泌的机制.第四军医大学学报,2001;22(9):786-789.
[10]常小荣,严洁,易受乡,等.电针足三里对大鼠胃电及脑肠肽的影响.中医药学刊,2004;22(2):220.
[11]牟向东,谢鹏雁,刘建湘.电针刺大鼠足三里穴对LESP血浆胃泌素和胃动素的影响.世界华人消化杂志,2005;13(9):1069-1073.
[12]常小荣,严洁,刘玉群,等.电针足三里和阳陵泉穴对家兔胃胆运动及脑肠肽的影响.世界华人消化杂志,2006;14(17):1662-1668.
[13]王伯沄,李玉松,黄高异,等.病理学技术.北京:人民卫生出版社,2000:61.
[14]姚永莉,宋于刚,张万岱.实验性大鼠胃肠道功能紊乱模型的建立.第一军医大 学学报,2001:21(5):338-340.
[15]Dougas A,Drossnan R.The functional gastrointestinal disorder and the Rome Ⅲprocess.Gastroenterology,2006;130:1377-1390.
[16]徐敏.功能性消化不良的发病机制.交通医学,2008;22(4):375-377.
[17]程珠琴,郑立升.中西医结合治疗胃肠动力障碍性疾病的进展.中医药学刊,2006;24(10):1894-1897.
[18]陶欣艺,方泰惠,徐立,等.肠泰颗粒对实验性胃肠道功能紊乱症的研究.南京中医药大学学报,2004;20(2):110-111.
[19]贾甄伟,赵宁,张皖东,等.理中丸和四君子汤对利血平脾虚大鼠自主活动的影响.中国中医药信息杂志,2006;13(5):34-35.
[20]封吉化,任平,肖青林,等.脾虚证大鼠甘草甜素药物动力学特征及其机制的探讨.武警医学,2003;14(6):326-328.
[21]张勇,王振华.大鼠胃电节律失常模型的建立.华人消化杂志,1998;6(7):612-613.
[22]夏立营,葛文津,李明喜.和胃消痞胶囊对大鼠胃肠肌问神经丛一氧化氮合酶的影响.中国中西医结合消化杂志,2001;9(5):259-260.
[23]李利生,曲瑞瑶,王伟,等.冷束缚应激大鼠十二指肠电-机械活动变化.首都医科大学学报,2002,12;23(4):300-302.
[24]杜鹃,柯道平,胡金兰,等.银杏叶提取物对大鼠不同状态胃动力的影响及其机制.临床消化病杂志,2008;20(5):281-283.
[25]徐焱琛,蓝森麟,陈津岩,等.黄芪四君汤对脾虚证大鼠甲状腺素及环核苷酸水平的影响.中药新药与临床药理,2007;18(4):291-293.
[26]陈琼科,王再谟.脾虚宁浸膏对脾虚泄泻动物模型红细胞免疫的影响.中国中西医结合消化杂志,2002;10(5):273-275.
[27]易崇勤,叶百宽,金敬善,等.四君子汤对脾虚大鼠粘膜酶组织化学及血浆胃肠激素的影响.北京中医药大学学报,1997;11(6):31-34.
[28]画伟.心理应激胃粘膜损伤的动物模型及发病机制.国外医学·消化系疾病分册,2002:3(22):143-145.
[29]Mazzon E,Stumiolo GC,Puzzolo D,et al.Effect of stress on the paracellular barrier in the rat ileum.Gut,2002;51:507-513.
[30]Li L,Liu H,Li YZ,et al.The human brain response to acupuncture on same-meridian acupoints:evidence from an fMRI study.JAltern Complement Med,2008;14(6):673-678.
[31]彭随风,杨家耀,时昭红.电针改善功能性消化不良胃动力、自主神经功能及心理状态.世界华人消化杂志,2008;16(36):4105-4109.
[32]Ji LX,Yah LP,Wang H J,et al.Selection of basic acupoints for composing "gastric-disorder-formula" for electroacupuncture prevention of acute gastric mucosal lesionin rats.Zhen Ci Yah Jiu,2008;33(5):296-300,325.
[33]秦明,黄裕新,王景杰,等.预防性针刺足三里穴对冷应激大鼠胃粘膜损伤的影响.西南国防医药,2005;15(2):133-136.
[34]Senna-Fernandes V,Franca DL,de Souza D,et al.Acupuncture at "Zusanli"(St.36)and "Sanyinjiao"(SP.6) Points on the Gastrointestinal Tract:A Study of the Bioavailability of 99mTc-Sodium Pertechnetate in Rats.Evid Based Complement Alternat Med.2009;12(2):Epub ahead of print.
[35]吕志平,刘承才.“肝郁”大鼠血浆TXA_2、PGI_2水平与肝微循环变化及逍遥散作用.中国微循环,2000:4(3):160-161.
[36]于德林,董少义.激光多普勒在神经外科的应用.武警医学院学报,1999;11(2):134-135.
[37]孙大勇,黄裕新,高巍,等.穴位电针影响狗血浆、胃粘膜组织中ET含量及意义.广州医学院学报,2002;30(2):14-16.
[38]孙大勇,黄裕新,高巍,等.电针对狗血浆部分脑肠肽含量的影响及意义.解放军医学杂志,2002;27(11):997-998.
[39]高希言,吕金秀,刘望乐,等.针灸对胃粘膜屏障功能影响的实验研究.中国中医药科技,2001;8(5):277-279.
[40]杨凤江,王景杰.针刺足三里穴对实验性脾虚大鼠胃粘膜血流量及胃肠激素水平的影响.武警医学,2008;19(8):716-718.
[41]Zhang D,Li SY,Ma HM,et al.Study on effect of electroacupuncture on gastric ischemia-reperfusion by laser Doppler blood perfusion imaging.Zhongguo Zhen Jiu,2007;27(11):833-838.
[42]申国明,江安宏,徐颖,等.电针对胃粘膜损伤大鼠血管活性肠肽影响的实验研究.中国中医药科技,2002;9(6):321-323.
[43]徐颖,中国明,吕磊,等.血管活性肠肽参与电针对大鼠胃粘膜损伤的保护作用.中国中西医结合消化杂志,2003;11(4):215-217.
[44]Xu GS,Sun Y,Wang ZJ,et al.Effects ofelectroacupuncture on gastric mucosal blood flow and transmucosal potential difference instress rats.World J China Gastroenterol,1998;6(1):4-6.
[45]林庶茹,才丽平,林大勇.电针“足三里”穴对胃粘膜损伤大鼠血流量及一氧化氮、内皮素影响的实验研究.针刺研究,2006;31(2):110-112.
[46]林主坤,胡品津,李初俊,等.功能性消化不良胃电图与胃窦部动力.中华消化杂志,2002;22(5):315-316.
[47]连至诚,欧阳守,许冠荪.胃肠电及胃肠动力研究在中国.广州:华南理工大学出版社,1997;335.
[48]周吕,王礼建,云天佑,等.电针胃经穴对狗胃肠移行性复合运动及脑肠肽释放的作用.科学通报,2000;45(22):2419-2426.
[49]常小荣,严洁,易受乡,等.电针“足三里”对大鼠胃电及脑肠肽的影响.中国针灸,2004;24(2):125-127.
[50]常小荣,严洁,易受乡,等.电针足三里对大鼠胃电及脑肠肽的影响.中医药学刊,2004;22(2):220.
[51]Sun GJ,Wang SJ,Du YJ,et al.Study on dual-directional regulation induced by acupuncture at different acupoints in the rat with motility abnormality of the stomach.Zhongguo Zhen Jiu,2007;27(11):839-842.
[52]王述菊,孙国杰,杜艳军,等.孤束核在针刺“足三里”调节胃运动中的作用机制.中国中医药信息杂志,2007;14(9):28-30.
[53]王述菊,孙国杰,杜艳军,等.针刺对胃运动异常大鼠调节作用的神经机制研究.中国中医急症,2008;17(3):352-354.
[54]黄裕新,王景杰,王胜智,等.胶质细胞参与电针足三里对胃运动的调节作用.山西医科大学学报,2007;38(10):886-890.
[55]于航,贾庆波,王晶.电针“足三里”穴对家兔胃运动、胃电的影响及与蓝斑核关系的研究.四川解剖学杂志,2004;12(3):208-211.
[56]陈香梅,刘志敏,王新梅,等.电针对家兔胃运动、胃电的影响及与中缝大核关系的研究.针灸临床杂志,2002;18(1):49.
[57]王晶,刘志敏,金淑英,等.电针足三里对胃运动胃电的抑制作用与蓝斑核的递质受体关系的研究.中医药学刊,2002;20(2):219.
[58]王景杰,夏德雨,卢王,等.针刺功能性消化不良患者足三罩穴对其胃运动功能的影响.胃肠病学和肝病学杂志,2008;17(7):561-563.
[59]邓元江,易受乡,林亚平,等.针刺足阳明经穴对家兔胃窦平滑肌细胞内钙离子浓度的影响.中医杂志,2004;15(8):587-590.
[60]黄碧群.针刺足三里对胃窦面积的超声观察.上海针灸杂志,2001;20(1):8-9.
[61]刘劲松,杨菊,侯晓华,等.寒冷浸水应激对大鼠胃排空及肠肌间神经递质的影响.中华消化杂志,2004;24(8):492-493.
[62]赵景涛,刘玉兰.消化内科学.北京:中国协和医科大学出版社,2000.
[63]方正清,申国明,徐颖,等.电针和半夏泻心汤对功能性消化不良大鼠胃肠电图的影响.安徽中医学院学报,2005;24(1):14-16.
[64]雷君,李琴,李国成,等.电针对大鼠胃肠动力障碍的调整作用及其神经化学机制.针刺研究,2005;30(3):131-137.
[65]向荣成,厉有名.神经胃肠病.北京:人民卫生出版社,2001.
[66]贾萍,陈刚,陈日新.电针“足三里”穴对家兔胃电节律紊乱调整的时间效应关系研究.四川中医,2007;25(3):12-14.
[67]Wang YG,Yao SK.Study on effects of low frequency pulse plus auricular point magnetic therapy on electrogastrogram and clinical therapeutic effect in the patient of functional dyspepsia.ZhongguoZhenJiu,2007;27(4):245-248.
[68]赵保民,黄裕新,郝悦,等.电针“足三里”和“三阴交”调节大鼠胃酸分泌的作用比较.针刺研究,2001;26(4):279-283.
[69]赵保民,黄裕新,张伟,等.电针对大鼠胃粘膜胃泌素和EGFR表达及AgNOK 的影响.第四军医大学学报,2001;22(9):782-785.
[70]严洁,常小荣,邓沅江,等.电针足阳明经穴对家兔胃粘膜损伤防御性保护作用的研究.中国针灸,2001;21(6):35.
[71]常小荣,严洁,林亚平,等.针刺足三里对功能性消化不良患者血浆胃肠激素的影响.中国中西医结合消化杂志,2001;9(5):283-284.
[72]胡柏平,范学辉.电针足三里穴对运动应激性溃疡大鼠几种脑肠肽含量的影响.中国体育科技,2008;44(2):133-136.
[73]吴湘,刘磊,郝悦,等.针刺“足三里”对实验性脾虚大鼠胃排空及胃肠激素的影响.实用医学杂志,2008;24(11):1896-1897.
[74]周国平,严洁,常小荣,等.电针足三里对胃粘膜损伤家兔前列腺素E2、胃泌素水平的影响.中国中医药科技,2004;11(1):1.
[75]Wang X,Teng JR,Lu B,et al.Effect of acupuncture of"Zusanli"(ST 36) on serum gastrin and cortisol contents in rats with spleen deficiency syndrome.Zhen Ci Yan Jiu,2007;32(2):125-127.
[76]Dou DP,Cai G.Regulation of gastric motility.Shijie Huaren Xiaohua Zazhi,1999;7:353.
[77]Zhou L,Wang LJ,Yuan B,et al.Effect of motilin on gastric smooth contraction induced by interstitial cells of Calal.Zhonghua Yixue Zazhi,2003;83:1422-1427.
[78]Huang J,Zhou H,Mahavadi S,et al.Signaling pathways mediating gastrointestinal smooth muscle contraction and MLC20 phosphorylation by motilin receptors.Am J Physiol GastrointestLiverPhysiol,2005;288:G23-31.
[79]严洁,常小荣,邓沅江,等.电针足阳明经穴对家兔胃粘膜损伤防御性保护作用的研究.中国针灸,2001;21(6):35.
[80]常小荣,严洁,林亚平,等.针刺足阳明经穴对健康人血浆胃动素及胃泌素含量的影响.中国中西医结合消化杂志,2001;9(2):69-70.
[81]Niu WX,He GD,Liu H,et al.Effects and probable mechanisms of electroacupuncture at the Zusanli point on upper gastrointestinal motility in rabbits.J Gastroenterol Hepatol,2007;22(10):1683-1699.
[82]易受乡,林亚平,严洁,等.电针对大鼠胃运动P物质和胃动素的影响.世界华人消化杂志,2001;9(3):284-287.
[83]方正清,张育琴,许冠荪.电针和半夏泻心汤对功能性消化不良大鼠胃动素的影响.广州中医药大学学报,2007;24(5):379-383.
[84]刘元全,王健,郝志友.针刺对功能性消化不良胃肠动力影响的临床研究.中国针灸,2001;21(5):267.
[85]Lin YP,Yi SX,Yan J,et al.Effect of acupuncture at Foot-Yangming Meridian on gastric mucosal blood flow,gastric motility and brain-gut peptide.World J Gastroenterol,2007;13(15):2229-2233.
[86]Iaquinto G,Giardullo N,Taccone W,et al.Role of endogenous endothelin-1 in ethanol-induced gastric mucosal damage in humans.Dig DisSci,2003;48:663-669.
[87]孙大勇,黄裕新,高巍,等.电针对狗血浆、胃粘膜组织中内皮素含量的影响及意义.针刺研究,2002;27(3):197-200.
[88]韩炜,郭成浩,李延青,等.肠易激综合征患者内皮素和一氧化氮含量变化的意义.胃肠病学,2002;7(6):342-345.
[89]易受乡,彭艳,常小荣,等.艾灸足三里、梁门穴对应激性溃疡大鼠胃粘膜细胞凋亡的干预作用.世界华人消化杂志,2006;14(33):3163-3168.
[90]李莉,盖全武,姚岚,等.电针抗大鼠急性胃粘膜损伤机理的实验研究.上海针灸杂志,2004;23(12):36-38.
[91]Jin JG,Murthy KS,Grider JR,Makhlouf GM.Characterization of PACAP receptors and signaling pathways in rabbit gastric muscle cells.Am J Physiol,1997;272:G1391-1399.
[92]Jin HO.Inhibition of acid secretion by electrical acupuncture is mediated via beta-endorphin and somatostatin.Am J Physiol,1996;271(3pt1):G524-G530.
[93]高巍,黄裕新,陈洪,等.电针足三里对大鼠垂体和外周血中脑肠肽含量的影响.第四军医大学学报,2001;22(22):2023-2025.
[94]高巍,黄裕新,陈洪,等.脑肠肽在针刺调节免疫反应中的作用.中国免疫学杂 志,2001;17:473-477.
[95]郭华,曲瑞瑶,常延滨,等.实验性脾虚证大鼠回肠P物质和血管活性肠肽含量的变化.首都医科大学学报,2001;22(3):199-201.
[96]李利生,曲瑞瑶,郭华,等.冷应激大鼠血浆及回肠P物质和VIP的变化.首都医科大学学报,2002;23(2):113-114.
[97]李利生,曲瑞瑶,王伟,等.实验性脾虚大鼠与冷应激大鼠回肠段SP、VIP含量的变化.深圳中西医结合杂志,2002;12(2):75-76.
[98]黄裕新,赵保民,张卫东,等.纳络酮和西咪替丁对电针大鼠足三里胃酸分泌效应的影响.第四军医大学学报,2001;22(9):825-827.
[99]刘凯,齐清会.实验性脾虚证平滑肌细胞VIP受体信号传导障碍.天津中医药,2003,20(6):52-55.
[100]徐颖,中国明,杨永兴,等.中枢VIP参与电针对大鼠胃黏膜的保护作用.安徽中医学院学报,2004;21(3):31-34.
[101]申国明,徐颖,吕磊,等.电针对冷应激致胃黏膜损伤大鼠VIP免疫反应性的影响.安徽中医学院学报,2004;23(3):29-32.
[102]李春元.5-HT受体调节剂在肠易激综合征治疗中的作用.世界临床药物,2003;24(5):309-310.
[103]Foxx-Orenstein AE,Kuemmerle JF,Grider JR.Distinct 5-HT receptors mediate the peristaltic reflex induced by mucosal stimuli in human and guinea pig intestine.Gastroenterology,1996;111:1281-1290.
[104]李兆申,詹丽杏,邹多武,等.肠易激综合征患者分泌5-羟色胺的肠嗜铬细胞形态及功能的改变.中华消化杂志,2004;24:94-97.
[105]Spiller RC,Jenkins D,Thomley JP,et al.Increased rectal mucosal entereendoefine cells T lymphoeytes and increased gut permeability following acute camplobacter enteritis and in post-dysenteric irritable bowel syndrome.Gut,2000;47(6):804.
[106]Houghton LA,Atkinson W,Lockhart S,et al.Sigmoid-colonic motility in health and irritable bowel syndrome:a role for 5-hydroxytryptamine.Neuro gastroenterol Motil,2007;19:724-731.
[107]刘志敏,陈宝忠,王东岩.中枢5-HT受体在电针对胃运动、胃电影响中的作用.上海针灸杂志,2000;19(4):41-42.
[108]陈香梅,柳滨,刘志敏.中缝大核微量注射5-HT等药物对电针抑制胃运动、胃电的影响.针灸临床杂志,2004;20(4):47-50.
[109]朱舜丽,许冠荪,王振玖,等.电针对应激大鼠5-HT、NOS及胃粘膜的影响.新消化病学杂志,1997;5(8):493.
[110]刘丽宏,梅其炳,陈蕾,等.5-羟色胺对培养胃底平滑肌细胞内游离钙动员的影响.第四军医大学学报,2001;22(10):891.
[111]李兆申,詹丽杏,赵兴刚,等.腹腔注射卵清白蛋白致大鼠内脏高敏感的研究.第二军医大学学报,2003;24(2):127-130.
[112]陈锡强,刘可春,韩利文,等.醋酸直肠刺激对大鼠肠道P物质、5-HT免疫阳性神经纤维的影响.中国实验方剂学杂志,2008;14(11):71-73.
[113]姜敏,凌立平,傅宝玉.肠易激综合征患者结肠5-HT的分布特点.世界华人消化杂志,2005;13(4):541-543.
[114]王静,赖华梅,褚奇,等.内脏高敏感大鼠肠道5-羟色胺转运体的变化以及中药肠吉安治疗的研究.胃肠病学,2008;13:18-21.
[115]王胜.针刺对实验性坐骨神经根压迫模型大鼠脑组织中单胺递质的影响.中国临床康复,2005;8(9):94-96.
[116]韩济生.中枢5-羟色胺在针刺镇痛中的作用.中国科学,1978;(5):578.
[117]Whitehead WE,Palsson O,Jones KR.Systematic review of the comorbidity of irritable bowel syndrome with other disorders:what are the causes and implications?Gastroenterology,2002;122:1140-1156.
[118]Schultz W.Getting formal with dopamine and reward.Neuron,2002;36(23):241-263.
[119]Dive A,Foret F,JamartJ,et al.Intensive Care Med,2000;26:901-907.
[120]Miyazawa T,Matsumoto M,Kato S,et al.Med Sci Monit,2003;9:71-77.
[121]Wiley J,Owyang C.J Pharmacol Exp Ther,1987;242:548-55.
[122]慈蕾,安书成.眶额叶区多巴胺对胃运动的影响及其机制研究.陕西师范大学学报(自然科学版),2007;35(1):99-102.
[123]朱舜丽,许冠荪,陈全珠,等.电针足三里穴对应激性胃溃疡大鼠一氧化氮和儿茶酚胺的影响.中国中西医结合脾胃杂志,1996;4(1):39.
[124]Posserud I,Agerforz P,EkmanR,et al.Altered visceral perceptual and neuroendocrine response inpatients with irritable bowel syndrome durine mental stress.Gut,2004;53:1102-1108.
[125]刘建中,伍忍,姚运纬.侧脑室微量注射去甲肾上腺素对大鼠应激性胃溃疡形成的影响.河南医科大学学报,1994;29,4.
[126]李家玲.第二届全国针灸针麻学术讨论会论文摘要,1984,334.
[127]王巍峰,杨云生,孙刚,等.肠易激综合征大鼠模型脊髓后角神经递质表达的改变.胃肠病学和肝病学杂志,2006;15(4):405-408.
[128]Ohtsuka T,Yokohata K,Inoue K,et al.Biliary sphincter motility after neural isolation of the pancreatoduodenal region in conscious dogs.Surgery,2002;131:139-148.
[1]Slomiany BL,Slomiany A.Induction of endothelin-converting enzymel in gastric mucosal injury by idomethacin.Biochem Biophys Res Commun,2000;269:377.
[2]孙大勇,黄裕新,高巍,等.穴位电针影响狗血浆、胃粘膜组织中ET含量及意义.广州医学院学报,2002;30(2):14-16.
[3]孙大勇,黄裕新,高巍,等.电针对狗血浆部分脑肠肽含量的影响及意义.解放军医学杂志,2002;27(11):997-998.
[4]高希言,吕金秀,刘望乐,等.针灸对胃粘膜屏障功能影响的实验研究.中国中医药科技,2001;8(5):277-279.
[5]张燕,于浩.针刺耳胃穴对大鼠乙酸实验性胃溃疡的保护作用及机理探讨.贵阳中医学院学报,1998;20(2):33-35.
[6]秦明,黄裕新,王景杰,等.预防性针刺足三里穴对冷应激大鼠胃粘膜损伤的影响.西南国防医药,2005;15(2):133-136.
[7]魏庆兴,菊地一博,郑义弘,等.针灸预防大鼠应激性胃溃疡作用的实验研究.中国针灸,1997;17(10):608.
[8]谢守鹏.胃必灵胶囊配合针刺对慢性萎缩性胃炎患者胃粘膜血流量的影响.湖北中医杂志,2006;28(4):6-7.
[9]林亚平,易受乡,严洁,等.电针足阳明经穴对胃粘膜损伤大鼠胃粘膜血流量及生长抑素的影响.中国中西医结合消化杂志,2003;11(2):75-77.
[10]刘涌,许冠荪.电针足三里对应激性胃粘膜损伤的保护作用.安徽中医学院学报,2000;19(2):27-30.
[11]杨凤江,王景杰.针刺足三里穴对实验性脾虚大鼠胃粘膜血流量及胃肠激素水平的影响.武警医学,2008;19(8):716-718.
[12]申国明,江安宏,徐颖,等.电针对胃粘膜损伤大鼠血管活性肠肽影响的实验研究.中国中医药科技,2002;9(6):321-323.
[13]徐颖,申国明,吕磊,等.血管活性肠肽参与电针对大鼠胃粘膜损伤的保护作用.中国中西医结合消化杂志,2003;11(4):215-217.
[14]杨永兴,许冠荪,沈德凯,等.DVC微量注射VIP对大鼠GMBF放大和电针调整效应.安徽中医学院学报,2001;20(4):38-40.
[15]林庶茹,才丽平,林大勇.电针“足三里”穴对胃粘膜损伤大鼠血流量及一氧化氮、内皮素影响的实验研究.针刺研究,2006;31(2):110-112.
[16]黄裕新,楮自宏,张少玲,等.针刺对血浆胃动素、生长抑素及内皮素的影响及意义.第四军医大学学报,1998;19(1):28-30.
[17]Xu GS,Sun Y,Wang ZJ,et al.Effects of electroacupuncture on gastric mucosal blood flow and transmucosal potential difference instress rats.World J China Gastroenterol,1998;6(1):4-6.
[18]杨琦,秦明,黄裕新,等.电针对应激大鼠胃粘膜血流及血浆ET,NO,CGRP 的影响.第四军医大学学报,2001;22(9):800-802.
[2]林亚平,易受乡,严洁,等.电针足阳明经穴对胃粘膜损伤大鼠胃粘膜血流量及生长抑素的影响.中国中西医结合消化杂志,2003;11(2):75-77.
[3]张泓,易受乡,严洁,等.电针足三阳经穴对家兔脑肠肽类物质影响的比较研究.中国临床康复.2004,12:2290-2291.
[4]英振吴.电针足三里穴对实验性胃肠道功能紊乱大鼠模型的影响及调理作用的研究.2007硕士学位论文全文数据库.
[5]邓春雷.实验针灸学.北京:人民卫生出版社,1998,第1版:7.
[6]姬广臣,史仁华,赵鲁鸣.电针对完全结扎双侧颈总动脉后大鼠软脑膜微循环血流量的影响.中国针灸,1997;17(10):606.
[7]连学建,王建武,郭莲军,等.胃克星对大鼠肠系膜微循环的影响.临床消化病杂志,2003;15(6):265-266.
[8]张丽,顾瑛,刘凡光,等.光动力作用对大鼠肠系膜微循环的影响.中国激光医学杂志,2003;5(12):69-75.
[9]赵保民,黄裕新,赵宁侠,等.电针调控大鼠胃酸分泌的机制.第四军医大学学报,2001;22(9):786-789.
[10]常小荣,严洁,易受乡,等.电针足三里对大鼠胃电及脑肠肽的影响.中医药学刊,2004;22(2):220.
[11]牟向东,谢鹏雁,刘建湘.电针刺大鼠足三里穴对LESP血浆胃泌素和胃动素的影响.世界华人消化杂志,2005;13(9):1069-1073.
[12]常小荣,严洁,刘玉群,等.电针足三里和阳陵泉穴对家兔胃胆运动及脑肠肽的影响.世界华人消化杂志,2006;14(17):1662-1668.
[13]王伯沄,李玉松,黄高异,等.病理学技术.北京:人民卫生出版社,2000:61.
[14]姚永莉,宋于刚,张万岱.实验性大鼠胃肠道功能紊乱模型的建立.第一军医大 学学报,2001:21(5):338-340.
[15]Dougas A,Drossnan R.The functional gastrointestinal disorder and the Rome Ⅲprocess.Gastroenterology,2006;130:1377-1390.
[16]徐敏.功能性消化不良的发病机制.交通医学,2008;22(4):375-377.
[17]程珠琴,郑立升.中西医结合治疗胃肠动力障碍性疾病的进展.中医药学刊,2006;24(10):1894-1897.
[18]陶欣艺,方泰惠,徐立,等.肠泰颗粒对实验性胃肠道功能紊乱症的研究.南京中医药大学学报,2004;20(2):110-111.
[19]贾甄伟,赵宁,张皖东,等.理中丸和四君子汤对利血平脾虚大鼠自主活动的影响.中国中医药信息杂志,2006;13(5):34-35.
[20]封吉化,任平,肖青林,等.脾虚证大鼠甘草甜素药物动力学特征及其机制的探讨.武警医学,2003;14(6):326-328.
[21]张勇,王振华.大鼠胃电节律失常模型的建立.华人消化杂志,1998;6(7):612-613.
[22]夏立营,葛文津,李明喜.和胃消痞胶囊对大鼠胃肠肌问神经丛一氧化氮合酶的影响.中国中西医结合消化杂志,2001;9(5):259-260.
[23]李利生,曲瑞瑶,王伟,等.冷束缚应激大鼠十二指肠电-机械活动变化.首都医科大学学报,2002,12;23(4):300-302.
[24]杜鹃,柯道平,胡金兰,等.银杏叶提取物对大鼠不同状态胃动力的影响及其机制.临床消化病杂志,2008;20(5):281-283.
[25]徐焱琛,蓝森麟,陈津岩,等.黄芪四君汤对脾虚证大鼠甲状腺素及环核苷酸水平的影响.中药新药与临床药理,2007;18(4):291-293.
[26]陈琼科,王再谟.脾虚宁浸膏对脾虚泄泻动物模型红细胞免疫的影响.中国中西医结合消化杂志,2002;10(5):273-275.
[27]易崇勤,叶百宽,金敬善,等.四君子汤对脾虚大鼠粘膜酶组织化学及血浆胃肠激素的影响.北京中医药大学学报,1997;11(6):31-34.
[28]画伟.心理应激胃粘膜损伤的动物模型及发病机制.国外医学·消化系疾病分册,2002:3(22):143-145.
[29]Mazzon E,Stumiolo GC,Puzzolo D,et al.Effect of stress on the paracellular barrier in the rat ileum.Gut,2002;51:507-513.
[30]Li L,Liu H,Li YZ,et al.The human brain response to acupuncture on same-meridian acupoints:evidence from an fMRI study.JAltern Complement Med,2008;14(6):673-678.
[31]彭随风,杨家耀,时昭红.电针改善功能性消化不良胃动力、自主神经功能及心理状态.世界华人消化杂志,2008;16(36):4105-4109.
[32]Ji LX,Yah LP,Wang H J,et al.Selection of basic acupoints for composing "gastric-disorder-formula" for electroacupuncture prevention of acute gastric mucosal lesionin rats.Zhen Ci Yah Jiu,2008;33(5):296-300,325.
[33]秦明,黄裕新,王景杰,等.预防性针刺足三里穴对冷应激大鼠胃粘膜损伤的影响.西南国防医药,2005;15(2):133-136.
[34]Senna-Fernandes V,Franca DL,de Souza D,et al.Acupuncture at "Zusanli"(St.36)and "Sanyinjiao"(SP.6) Points on the Gastrointestinal Tract:A Study of the Bioavailability of 99mTc-Sodium Pertechnetate in Rats.Evid Based Complement Alternat Med.2009;12(2):Epub ahead of print.
[35]吕志平,刘承才.“肝郁”大鼠血浆TXA_2、PGI_2水平与肝微循环变化及逍遥散作用.中国微循环,2000:4(3):160-161.
[36]于德林,董少义.激光多普勒在神经外科的应用.武警医学院学报,1999;11(2):134-135.
[37]孙大勇,黄裕新,高巍,等.穴位电针影响狗血浆、胃粘膜组织中ET含量及意义.广州医学院学报,2002;30(2):14-16.
[38]孙大勇,黄裕新,高巍,等.电针对狗血浆部分脑肠肽含量的影响及意义.解放军医学杂志,2002;27(11):997-998.
[39]高希言,吕金秀,刘望乐,等.针灸对胃粘膜屏障功能影响的实验研究.中国中医药科技,2001;8(5):277-279.
[40]杨凤江,王景杰.针刺足三里穴对实验性脾虚大鼠胃粘膜血流量及胃肠激素水平的影响.武警医学,2008;19(8):716-718.
[41]Zhang D,Li SY,Ma HM,et al.Study on effect of electroacupuncture on gastric ischemia-reperfusion by laser Doppler blood perfusion imaging.Zhongguo Zhen Jiu,2007;27(11):833-838.
[42]申国明,江安宏,徐颖,等.电针对胃粘膜损伤大鼠血管活性肠肽影响的实验研究.中国中医药科技,2002;9(6):321-323.
[43]徐颖,中国明,吕磊,等.血管活性肠肽参与电针对大鼠胃粘膜损伤的保护作用.中国中西医结合消化杂志,2003;11(4):215-217.
[44]Xu GS,Sun Y,Wang ZJ,et al.Effects ofelectroacupuncture on gastric mucosal blood flow and transmucosal potential difference instress rats.World J China Gastroenterol,1998;6(1):4-6.
[45]林庶茹,才丽平,林大勇.电针“足三里”穴对胃粘膜损伤大鼠血流量及一氧化氮、内皮素影响的实验研究.针刺研究,2006;31(2):110-112.
[46]林主坤,胡品津,李初俊,等.功能性消化不良胃电图与胃窦部动力.中华消化杂志,2002;22(5):315-316.
[47]连至诚,欧阳守,许冠荪.胃肠电及胃肠动力研究在中国.广州:华南理工大学出版社,1997;335.
[48]周吕,王礼建,云天佑,等.电针胃经穴对狗胃肠移行性复合运动及脑肠肽释放的作用.科学通报,2000;45(22):2419-2426.
[49]常小荣,严洁,易受乡,等.电针“足三里”对大鼠胃电及脑肠肽的影响.中国针灸,2004;24(2):125-127.
[50]常小荣,严洁,易受乡,等.电针足三里对大鼠胃电及脑肠肽的影响.中医药学刊,2004;22(2):220.
[51]Sun GJ,Wang SJ,Du YJ,et al.Study on dual-directional regulation induced by acupuncture at different acupoints in the rat with motility abnormality of the stomach.Zhongguo Zhen Jiu,2007;27(11):839-842.
[52]王述菊,孙国杰,杜艳军,等.孤束核在针刺“足三里”调节胃运动中的作用机制.中国中医药信息杂志,2007;14(9):28-30.
[53]王述菊,孙国杰,杜艳军,等.针刺对胃运动异常大鼠调节作用的神经机制研究.中国中医急症,2008;17(3):352-354.
[54]黄裕新,王景杰,王胜智,等.胶质细胞参与电针足三里对胃运动的调节作用.山西医科大学学报,2007;38(10):886-890.
[55]于航,贾庆波,王晶.电针“足三里”穴对家兔胃运动、胃电的影响及与蓝斑核关系的研究.四川解剖学杂志,2004;12(3):208-211.
[56]陈香梅,刘志敏,王新梅,等.电针对家兔胃运动、胃电的影响及与中缝大核关系的研究.针灸临床杂志,2002;18(1):49.
[57]王晶,刘志敏,金淑英,等.电针足三里对胃运动胃电的抑制作用与蓝斑核的递质受体关系的研究.中医药学刊,2002;20(2):219.
[58]王景杰,夏德雨,卢王,等.针刺功能性消化不良患者足三罩穴对其胃运动功能的影响.胃肠病学和肝病学杂志,2008;17(7):561-563.
[59]邓元江,易受乡,林亚平,等.针刺足阳明经穴对家兔胃窦平滑肌细胞内钙离子浓度的影响.中医杂志,2004;15(8):587-590.
[60]黄碧群.针刺足三里对胃窦面积的超声观察.上海针灸杂志,2001;20(1):8-9.
[61]刘劲松,杨菊,侯晓华,等.寒冷浸水应激对大鼠胃排空及肠肌间神经递质的影响.中华消化杂志,2004;24(8):492-493.
[62]赵景涛,刘玉兰.消化内科学.北京:中国协和医科大学出版社,2000.
[63]方正清,申国明,徐颖,等.电针和半夏泻心汤对功能性消化不良大鼠胃肠电图的影响.安徽中医学院学报,2005;24(1):14-16.
[64]雷君,李琴,李国成,等.电针对大鼠胃肠动力障碍的调整作用及其神经化学机制.针刺研究,2005;30(3):131-137.
[65]向荣成,厉有名.神经胃肠病.北京:人民卫生出版社,2001.
[66]贾萍,陈刚,陈日新.电针“足三里”穴对家兔胃电节律紊乱调整的时间效应关系研究.四川中医,2007;25(3):12-14.
[67]Wang YG,Yao SK.Study on effects of low frequency pulse plus auricular point magnetic therapy on electrogastrogram and clinical therapeutic effect in the patient of functional dyspepsia.ZhongguoZhenJiu,2007;27(4):245-248.
[68]赵保民,黄裕新,郝悦,等.电针“足三里”和“三阴交”调节大鼠胃酸分泌的作用比较.针刺研究,2001;26(4):279-283.
[69]赵保民,黄裕新,张伟,等.电针对大鼠胃粘膜胃泌素和EGFR表达及AgNOK 的影响.第四军医大学学报,2001;22(9):782-785.
[70]严洁,常小荣,邓沅江,等.电针足阳明经穴对家兔胃粘膜损伤防御性保护作用的研究.中国针灸,2001;21(6):35.
[71]常小荣,严洁,林亚平,等.针刺足三里对功能性消化不良患者血浆胃肠激素的影响.中国中西医结合消化杂志,2001;9(5):283-284.
[72]胡柏平,范学辉.电针足三里穴对运动应激性溃疡大鼠几种脑肠肽含量的影响.中国体育科技,2008;44(2):133-136.
[73]吴湘,刘磊,郝悦,等.针刺“足三里”对实验性脾虚大鼠胃排空及胃肠激素的影响.实用医学杂志,2008;24(11):1896-1897.
[74]周国平,严洁,常小荣,等.电针足三里对胃粘膜损伤家兔前列腺素E2、胃泌素水平的影响.中国中医药科技,2004;11(1):1.
[75]Wang X,Teng JR,Lu B,et al.Effect of acupuncture of"Zusanli"(ST 36) on serum gastrin and cortisol contents in rats with spleen deficiency syndrome.Zhen Ci Yan Jiu,2007;32(2):125-127.
[76]Dou DP,Cai G.Regulation of gastric motility.Shijie Huaren Xiaohua Zazhi,1999;7:353.
[77]Zhou L,Wang LJ,Yuan B,et al.Effect of motilin on gastric smooth contraction induced by interstitial cells of Calal.Zhonghua Yixue Zazhi,2003;83:1422-1427.
[78]Huang J,Zhou H,Mahavadi S,et al.Signaling pathways mediating gastrointestinal smooth muscle contraction and MLC20 phosphorylation by motilin receptors.Am J Physiol GastrointestLiverPhysiol,2005;288:G23-31.
[79]严洁,常小荣,邓沅江,等.电针足阳明经穴对家兔胃粘膜损伤防御性保护作用的研究.中国针灸,2001;21(6):35.
[80]常小荣,严洁,林亚平,等.针刺足阳明经穴对健康人血浆胃动素及胃泌素含量的影响.中国中西医结合消化杂志,2001;9(2):69-70.
[81]Niu WX,He GD,Liu H,et al.Effects and probable mechanisms of electroacupuncture at the Zusanli point on upper gastrointestinal motility in rabbits.J Gastroenterol Hepatol,2007;22(10):1683-1699.
[82]易受乡,林亚平,严洁,等.电针对大鼠胃运动P物质和胃动素的影响.世界华人消化杂志,2001;9(3):284-287.
[83]方正清,张育琴,许冠荪.电针和半夏泻心汤对功能性消化不良大鼠胃动素的影响.广州中医药大学学报,2007;24(5):379-383.
[84]刘元全,王健,郝志友.针刺对功能性消化不良胃肠动力影响的临床研究.中国针灸,2001;21(5):267.
[85]Lin YP,Yi SX,Yan J,et al.Effect of acupuncture at Foot-Yangming Meridian on gastric mucosal blood flow,gastric motility and brain-gut peptide.World J Gastroenterol,2007;13(15):2229-2233.
[86]Iaquinto G,Giardullo N,Taccone W,et al.Role of endogenous endothelin-1 in ethanol-induced gastric mucosal damage in humans.Dig DisSci,2003;48:663-669.
[87]孙大勇,黄裕新,高巍,等.电针对狗血浆、胃粘膜组织中内皮素含量的影响及意义.针刺研究,2002;27(3):197-200.
[88]韩炜,郭成浩,李延青,等.肠易激综合征患者内皮素和一氧化氮含量变化的意义.胃肠病学,2002;7(6):342-345.
[89]易受乡,彭艳,常小荣,等.艾灸足三里、梁门穴对应激性溃疡大鼠胃粘膜细胞凋亡的干预作用.世界华人消化杂志,2006;14(33):3163-3168.
[90]李莉,盖全武,姚岚,等.电针抗大鼠急性胃粘膜损伤机理的实验研究.上海针灸杂志,2004;23(12):36-38.
[91]Jin JG,Murthy KS,Grider JR,Makhlouf GM.Characterization of PACAP receptors and signaling pathways in rabbit gastric muscle cells.Am J Physiol,1997;272:G1391-1399.
[92]Jin HO.Inhibition of acid secretion by electrical acupuncture is mediated via beta-endorphin and somatostatin.Am J Physiol,1996;271(3pt1):G524-G530.
[93]高巍,黄裕新,陈洪,等.电针足三里对大鼠垂体和外周血中脑肠肽含量的影响.第四军医大学学报,2001;22(22):2023-2025.
[94]高巍,黄裕新,陈洪,等.脑肠肽在针刺调节免疫反应中的作用.中国免疫学杂 志,2001;17:473-477.
[95]郭华,曲瑞瑶,常延滨,等.实验性脾虚证大鼠回肠P物质和血管活性肠肽含量的变化.首都医科大学学报,2001;22(3):199-201.
[96]李利生,曲瑞瑶,郭华,等.冷应激大鼠血浆及回肠P物质和VIP的变化.首都医科大学学报,2002;23(2):113-114.
[97]李利生,曲瑞瑶,王伟,等.实验性脾虚大鼠与冷应激大鼠回肠段SP、VIP含量的变化.深圳中西医结合杂志,2002;12(2):75-76.
[98]黄裕新,赵保民,张卫东,等.纳络酮和西咪替丁对电针大鼠足三里胃酸分泌效应的影响.第四军医大学学报,2001;22(9):825-827.
[99]刘凯,齐清会.实验性脾虚证平滑肌细胞VIP受体信号传导障碍.天津中医药,2003,20(6):52-55.
[100]徐颖,中国明,杨永兴,等.中枢VIP参与电针对大鼠胃黏膜的保护作用.安徽中医学院学报,2004;21(3):31-34.
[101]申国明,徐颖,吕磊,等.电针对冷应激致胃黏膜损伤大鼠VIP免疫反应性的影响.安徽中医学院学报,2004;23(3):29-32.
[102]李春元.5-HT受体调节剂在肠易激综合征治疗中的作用.世界临床药物,2003;24(5):309-310.
[103]Foxx-Orenstein AE,Kuemmerle JF,Grider JR.Distinct 5-HT receptors mediate the peristaltic reflex induced by mucosal stimuli in human and guinea pig intestine.Gastroenterology,1996;111:1281-1290.
[104]李兆申,詹丽杏,邹多武,等.肠易激综合征患者分泌5-羟色胺的肠嗜铬细胞形态及功能的改变.中华消化杂志,2004;24:94-97.
[105]Spiller RC,Jenkins D,Thomley JP,et al.Increased rectal mucosal entereendoefine cells T lymphoeytes and increased gut permeability following acute camplobacter enteritis and in post-dysenteric irritable bowel syndrome.Gut,2000;47(6):804.
[106]Houghton LA,Atkinson W,Lockhart S,et al.Sigmoid-colonic motility in health and irritable bowel syndrome:a role for 5-hydroxytryptamine.Neuro gastroenterol Motil,2007;19:724-731.
[107]刘志敏,陈宝忠,王东岩.中枢5-HT受体在电针对胃运动、胃电影响中的作用.上海针灸杂志,2000;19(4):41-42.
[108]陈香梅,柳滨,刘志敏.中缝大核微量注射5-HT等药物对电针抑制胃运动、胃电的影响.针灸临床杂志,2004;20(4):47-50.
[109]朱舜丽,许冠荪,王振玖,等.电针对应激大鼠5-HT、NOS及胃粘膜的影响.新消化病学杂志,1997;5(8):493.
[110]刘丽宏,梅其炳,陈蕾,等.5-羟色胺对培养胃底平滑肌细胞内游离钙动员的影响.第四军医大学学报,2001;22(10):891.
[111]李兆申,詹丽杏,赵兴刚,等.腹腔注射卵清白蛋白致大鼠内脏高敏感的研究.第二军医大学学报,2003;24(2):127-130.
[112]陈锡强,刘可春,韩利文,等.醋酸直肠刺激对大鼠肠道P物质、5-HT免疫阳性神经纤维的影响.中国实验方剂学杂志,2008;14(11):71-73.
[113]姜敏,凌立平,傅宝玉.肠易激综合征患者结肠5-HT的分布特点.世界华人消化杂志,2005;13(4):541-543.
[114]王静,赖华梅,褚奇,等.内脏高敏感大鼠肠道5-羟色胺转运体的变化以及中药肠吉安治疗的研究.胃肠病学,2008;13:18-21.
[115]王胜.针刺对实验性坐骨神经根压迫模型大鼠脑组织中单胺递质的影响.中国临床康复,2005;8(9):94-96.
[116]韩济生.中枢5-羟色胺在针刺镇痛中的作用.中国科学,1978;(5):578.
[117]Whitehead WE,Palsson O,Jones KR.Systematic review of the comorbidity of irritable bowel syndrome with other disorders:what are the causes and implications?Gastroenterology,2002;122:1140-1156.
[118]Schultz W.Getting formal with dopamine and reward.Neuron,2002;36(23):241-263.
[119]Dive A,Foret F,JamartJ,et al.Intensive Care Med,2000;26:901-907.
[120]Miyazawa T,Matsumoto M,Kato S,et al.Med Sci Monit,2003;9:71-77.
[121]Wiley J,Owyang C.J Pharmacol Exp Ther,1987;242:548-55.
[122]慈蕾,安书成.眶额叶区多巴胺对胃运动的影响及其机制研究.陕西师范大学学报(自然科学版),2007;35(1):99-102.
[123]朱舜丽,许冠荪,陈全珠,等.电针足三里穴对应激性胃溃疡大鼠一氧化氮和儿茶酚胺的影响.中国中西医结合脾胃杂志,1996;4(1):39.
[124]Posserud I,Agerforz P,EkmanR,et al.Altered visceral perceptual and neuroendocrine response inpatients with irritable bowel syndrome durine mental stress.Gut,2004;53:1102-1108.
[125]刘建中,伍忍,姚运纬.侧脑室微量注射去甲肾上腺素对大鼠应激性胃溃疡形成的影响.河南医科大学学报,1994;29,4.
[126]李家玲.第二届全国针灸针麻学术讨论会论文摘要,1984,334.
[127]王巍峰,杨云生,孙刚,等.肠易激综合征大鼠模型脊髓后角神经递质表达的改变.胃肠病学和肝病学杂志,2006;15(4):405-408.
[128]Ohtsuka T,Yokohata K,Inoue K,et al.Biliary sphincter motility after neural isolation of the pancreatoduodenal region in conscious dogs.Surgery,2002;131:139-148.
[1]Slomiany BL,Slomiany A.Induction of endothelin-converting enzymel in gastric mucosal injury by idomethacin.Biochem Biophys Res Commun,2000;269:377.
[2]孙大勇,黄裕新,高巍,等.穴位电针影响狗血浆、胃粘膜组织中ET含量及意义.广州医学院学报,2002;30(2):14-16.
[3]孙大勇,黄裕新,高巍,等.电针对狗血浆部分脑肠肽含量的影响及意义.解放军医学杂志,2002;27(11):997-998.
[4]高希言,吕金秀,刘望乐,等.针灸对胃粘膜屏障功能影响的实验研究.中国中医药科技,2001;8(5):277-279.
[5]张燕,于浩.针刺耳胃穴对大鼠乙酸实验性胃溃疡的保护作用及机理探讨.贵阳中医学院学报,1998;20(2):33-35.
[6]秦明,黄裕新,王景杰,等.预防性针刺足三里穴对冷应激大鼠胃粘膜损伤的影响.西南国防医药,2005;15(2):133-136.
[7]魏庆兴,菊地一博,郑义弘,等.针灸预防大鼠应激性胃溃疡作用的实验研究.中国针灸,1997;17(10):608.
[8]谢守鹏.胃必灵胶囊配合针刺对慢性萎缩性胃炎患者胃粘膜血流量的影响.湖北中医杂志,2006;28(4):6-7.
[9]林亚平,易受乡,严洁,等.电针足阳明经穴对胃粘膜损伤大鼠胃粘膜血流量及生长抑素的影响.中国中西医结合消化杂志,2003;11(2):75-77.
[10]刘涌,许冠荪.电针足三里对应激性胃粘膜损伤的保护作用.安徽中医学院学报,2000;19(2):27-30.
[11]杨凤江,王景杰.针刺足三里穴对实验性脾虚大鼠胃粘膜血流量及胃肠激素水平的影响.武警医学,2008;19(8):716-718.
[12]申国明,江安宏,徐颖,等.电针对胃粘膜损伤大鼠血管活性肠肽影响的实验研究.中国中医药科技,2002;9(6):321-323.
[13]徐颖,申国明,吕磊,等.血管活性肠肽参与电针对大鼠胃粘膜损伤的保护作用.中国中西医结合消化杂志,2003;11(4):215-217.
[14]杨永兴,许冠荪,沈德凯,等.DVC微量注射VIP对大鼠GMBF放大和电针调整效应.安徽中医学院学报,2001;20(4):38-40.
[15]林庶茹,才丽平,林大勇.电针“足三里”穴对胃粘膜损伤大鼠血流量及一氧化氮、内皮素影响的实验研究.针刺研究,2006;31(2):110-112.
[16]黄裕新,楮自宏,张少玲,等.针刺对血浆胃动素、生长抑素及内皮素的影响及意义.第四军医大学学报,1998;19(1):28-30.
[17]Xu GS,Sun Y,Wang ZJ,et al.Effects of electroacupuncture on gastric mucosal blood flow and transmucosal potential difference instress rats.World J China Gastroenterol,1998;6(1):4-6.
[18]杨琦,秦明,黄裕新,等.电针对应激大鼠胃粘膜血流及血浆ET,NO,CGRP 的影响.第四军医大学学报,2001;22(9):800-802.