NM23-H1与IGF-Ⅱ在大肠癌中的表达及其意义
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摘要
目的:检测NM23—H1和16F—Ⅱ在大肠癌组织中的表达,分析它们与临床病例特征之间的关系及二者的相关性,探讨NM23—H1和IGF—Ⅱ在大肠癌进展、转移及预后中的作用。
方法:本实验采用免疫组化(SP三步法)检测NM23—H1和IGF—Ⅱ在正常大肠粘膜组织、大肠癌癌旁粘膜组织及大肠癌组织中的表达情况,分析它们与大肠癌临床病例特征之间的关系及二者的相关性。
结果:
1.NM23—H1在大肠癌组织中表达的阳性率为44.8%,在癌旁粘膜组织表达的阳性率为79.3%,正常肠粘膜组织表达的阳性率为90%,NM23-H1在癌组织中的表达明显低于癌旁粘膜组织和正常组织(p＜0.01);
2.IGF—Ⅱ在大肠癌组织中表达的阳性率为63.8%,在癌旁粘膜组织表达的阳性率为27.6%,正常大肠粘膜组织表达的阳性率为20%,IGF—Ⅱ在癌组织中的表达明显高于癌旁粘膜组织和正常组织(p＜0.01);
3.NM23-H1的表达与大肠癌患者的年龄、性别以及肿瘤大小和肿瘤分化程度无关;但与肿瘤的Dukes分期以及淋巴结转移(均p=0.011)有关。
4.IGF—Ⅱ的表达与大肠癌病人的年龄、性别、肿瘤大小及肿瘤分化程度无关;但与肿瘤Dukes分期以及淋巴结转移(均p=0.009)有关。
5.大肠癌组织中NM23—H1和IGF—Ⅱ表达呈负相关(r=-0.547,p＜0.001)
结论:
1.与癌旁粘膜组织及正常肠粘膜相比,NM23-H1在大肠癌组织中呈低表达,NM23-H1的低表达与大肠癌的Dukes分期以及淋巴结转移有关,而与大肠癌患者的年龄、性别、肿瘤大小及肿瘤分化程度无关。
2.与癌旁粘膜组织及正常肠粘膜相比,IGF—Ⅱ在大肠癌组织中呈高表达,IGF—Ⅱ的高表达与大肠癌的Dukes分期以及淋巴结转移有关,而与大肠癌患者的年龄、性别、肿瘤大小以及分化程度无关。
3.大肠癌组织中IGF—Ⅱ和NM23-H1表达呈负相关
OBJECTIVE: To investigate the expression of NM23-H1 and IGF-II in human colorectal carcinoma tissue, detect the relationship between them and clinical characteristics and analyze the relevance between IGF-II and NM23-H1.
METHODS: NM23-H1 and IGF-II expression were examined by immunohistochemistry (SP-method) in normal colorectal mucosa、paratumoral tissues and colorectal cancer tissue. The relationship between NM23-H1 and IGF-II expression and clinicopathological factors was statistically analyzed. The relevance of IGF- II and NM23-H1 was also observed.
RESULTS:
1. In colorectal cancer tissue, the positive expression rate of NM23-H1 was 44.8%; in paratumoral tissues, the positive expression rate was 79.3%, in normal colorectal mucosa tissues, the positive expression rate was 90%.The expression of NM23-H1 in cancer tissues was significantly lower than that in the paratumoral tissues and normal colorectal mucosa tissues (p <0.01).
2. In colorectal cancer tissues, the positive expression rate of IGF-II was 63.8%; in paratumoral tissues, the positive expression rate was 27.6%, In normal colon mucosa tissues, the positive expression rate was 20%. IGF- II protein in cancer tissue's expression was significantly higher than that in the paratumoral tissues and in normal colorectal mucosa tissues (P <0.01).
3. The expression of NM23-H1 in colorectal cancer tissues have no relationship with the age, gender, and tumor size and the degree of tumor differentiation. Decrease or loss of NM23-H1 expression was significantly correlated with Dukes staging of tumors (p=0.011) and lymph nodes metastasis (p=0.011).
4. The expression of IGF- II in colorectal cancer tissues have no relationship with the age, gender, tumor size and the degree of tumor differentiation. High IGF- II expression was significantly associated with Dukes staging of tumors (p = 0.009) and lymph nodes metastasis (p=0.009).
5. In colorectal cancer tissues, the negative correlation existed between NM23-H1 and IGF- II expression (r=-0.547,p <0.001).
CONCLUSIONS:
1. In colorectal cancer tissues NM23-H1 expression is lower significantly than expression in normal intestinal mucosa tissues and paratumoral intestinal mucosa tissues. NM23-H1 expression was significantly associated with tumor Dukes staging and lymph nodes metastasis, not correlated with age, gender, tumor size and the degree of tumor differentiation.
2. In colorectal cancer tissues IGF- II expression is higher significantly than expression in normal intestinal mucosa tissues and paratumoral intestinal mucosa tissues. IGF- II expression was significantly associated with tumor Dukes staging and lymph nodes metastasis , not correlated with the age, gender and tumor size and degree of tumor differentiation.
3. In colorectal cancer tissues, IGF- II and NM23-H1 expression was negatively correlated.
方法:本实验采用免疫组化(SP三步法)检测NM23—H1和IGF—Ⅱ在正常大肠粘膜组织、大肠癌癌旁粘膜组织及大肠癌组织中的表达情况,分析它们与大肠癌临床病例特征之间的关系及二者的相关性。
结果:
1.NM23—H1在大肠癌组织中表达的阳性率为44.8%,在癌旁粘膜组织表达的阳性率为79.3%,正常肠粘膜组织表达的阳性率为90%,NM23-H1在癌组织中的表达明显低于癌旁粘膜组织和正常组织(p＜0.01);
2.IGF—Ⅱ在大肠癌组织中表达的阳性率为63.8%,在癌旁粘膜组织表达的阳性率为27.6%,正常大肠粘膜组织表达的阳性率为20%,IGF—Ⅱ在癌组织中的表达明显高于癌旁粘膜组织和正常组织(p＜0.01);
3.NM23-H1的表达与大肠癌患者的年龄、性别以及肿瘤大小和肿瘤分化程度无关;但与肿瘤的Dukes分期以及淋巴结转移(均p=0.011)有关。
4.IGF—Ⅱ的表达与大肠癌病人的年龄、性别、肿瘤大小及肿瘤分化程度无关;但与肿瘤Dukes分期以及淋巴结转移(均p=0.009)有关。
5.大肠癌组织中NM23—H1和IGF—Ⅱ表达呈负相关(r=-0.547,p＜0.001)
结论:
1.与癌旁粘膜组织及正常肠粘膜相比,NM23-H1在大肠癌组织中呈低表达,NM23-H1的低表达与大肠癌的Dukes分期以及淋巴结转移有关,而与大肠癌患者的年龄、性别、肿瘤大小及肿瘤分化程度无关。
2.与癌旁粘膜组织及正常肠粘膜相比,IGF—Ⅱ在大肠癌组织中呈高表达,IGF—Ⅱ的高表达与大肠癌的Dukes分期以及淋巴结转移有关,而与大肠癌患者的年龄、性别、肿瘤大小以及分化程度无关。
3.大肠癌组织中IGF—Ⅱ和NM23-H1表达呈负相关
OBJECTIVE: To investigate the expression of NM23-H1 and IGF-II in human colorectal carcinoma tissue, detect the relationship between them and clinical characteristics and analyze the relevance between IGF-II and NM23-H1.
METHODS: NM23-H1 and IGF-II expression were examined by immunohistochemistry (SP-method) in normal colorectal mucosa、paratumoral tissues and colorectal cancer tissue. The relationship between NM23-H1 and IGF-II expression and clinicopathological factors was statistically analyzed. The relevance of IGF- II and NM23-H1 was also observed.
RESULTS:
1. In colorectal cancer tissue, the positive expression rate of NM23-H1 was 44.8%; in paratumoral tissues, the positive expression rate was 79.3%, in normal colorectal mucosa tissues, the positive expression rate was 90%.The expression of NM23-H1 in cancer tissues was significantly lower than that in the paratumoral tissues and normal colorectal mucosa tissues (p <0.01).
2. In colorectal cancer tissues, the positive expression rate of IGF-II was 63.8%; in paratumoral tissues, the positive expression rate was 27.6%, In normal colon mucosa tissues, the positive expression rate was 20%. IGF- II protein in cancer tissue's expression was significantly higher than that in the paratumoral tissues and in normal colorectal mucosa tissues (P <0.01).
3. The expression of NM23-H1 in colorectal cancer tissues have no relationship with the age, gender, and tumor size and the degree of tumor differentiation. Decrease or loss of NM23-H1 expression was significantly correlated with Dukes staging of tumors (p=0.011) and lymph nodes metastasis (p=0.011).
4. The expression of IGF- II in colorectal cancer tissues have no relationship with the age, gender, tumor size and the degree of tumor differentiation. High IGF- II expression was significantly associated with Dukes staging of tumors (p = 0.009) and lymph nodes metastasis (p=0.009).
5. In colorectal cancer tissues, the negative correlation existed between NM23-H1 and IGF- II expression (r=-0.547,p <0.001).
CONCLUSIONS:
1. In colorectal cancer tissues NM23-H1 expression is lower significantly than expression in normal intestinal mucosa tissues and paratumoral intestinal mucosa tissues. NM23-H1 expression was significantly associated with tumor Dukes staging and lymph nodes metastasis, not correlated with age, gender, tumor size and the degree of tumor differentiation.
2. In colorectal cancer tissues IGF- II expression is higher significantly than expression in normal intestinal mucosa tissues and paratumoral intestinal mucosa tissues. IGF- II expression was significantly associated with tumor Dukes staging and lymph nodes metastasis , not correlated with the age, gender and tumor size and degree of tumor differentiation.
3. In colorectal cancer tissues, IGF- II and NM23-H1 expression was negatively correlated.
引文
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