逆灸对心肌缺血再灌注损伤大鼠HSP70的影响
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摘要
目的:观察逆灸内关、膻中穴对心肌缺血再灌注损伤大鼠心肌组织中HSP70表达及组织结构改变的影响,旨在揭示逆灸对心肌的保护作用,为艾灸“治未病”理论提供科学实验依据。
方法:采用在体鼠心肌缺血再灌注损伤模型。将30只健康雄性Wistar大鼠,随机分成5组:缺血再灌注组——0号医用缝合线结扎冠状动脉左前降支,缺血20分钟,再灌注40分钟;缺血预处理组——缺血5分钟,再灌注5分钟,反复三次,后同上组;逆灸组——缺血再灌注前七天,艾灸“内关”“膻中”,每天2次,每次30分钟,后同上组;假手术组——手术仅以0号医用缝合线绕过冠状动脉左前降支,不予结扎;正常对照组——正常饲养。HE染色观察组织形态学变化。光镜观察心肌组织超微结构,免疫组织化学法检测各组心肌HSP70的含量表达。
结果:逆灸组(PM)、缺血预处理组(IP)HSP70表达水平明显增高,与缺血再灌注组(IR)相比有显著性差异(P＜0.05);预处理组(逆灸、缺血)与正常对照组及假手术组相比,各项指标有显著性差异(P＜0.05);逆灸组(PM)与缺血预处理组(IP)比较,各项指标无明显差异(P＞0.05)。
结论:逆灸对心肌缺血再灌注损伤大鼠心肌具有保护作用;逆灸可以增加HSP70的表达;逆灸作为一种心肌缺血的预处理手段,可以诱导心肌缺血耐受,减轻心肌缺血再灌注对心肌造成的损伤。
Objective: To observe the effects of preventive moxibustion on "Nei Guan"and "Tan Zhong" on expression of heat shock protein 70 and on the general structure histomorphology, to reveal the protective mechanism of preventive moxibustion on rat myocardial ischemia-reperfusion injury, then provide scientific experiment evidence.
Method: Using rat ischemia-reperfusion injury model in vivo, thirty male Wistar rats were randomly divided into five groups .Purely Ischemia-reperfusion group: the chest was opened and left anterior coronary was occluded by suture silk ,the occlusion was maintained for 20 minutes and released with reperfusion for 40 minutes ;Ischemic preconditioning Group (IP) : prior to ischemic reperfusion ,three cycles of 5 minutes of myocardial ischemia follow by 5 minutes of myocardial reperfusion , after which the procedures were the same to ditto; Preventive moxibustion Group(PM): 7 days prior to ischemic reperfusion ,moxibustion was given on "Nei Guan" "Tan Zhong" ,30 minutes twice daily .After which the procedures were the same to ditto .Sham Operating Group : the chest was opened and left anterior coronary was exposed with no further procedures; Control Group: Cadiocyte HE staining to observe change of histomorphology, Ultra structural pathological changes were observed with light microscope and the expression of heat shock protein 70 associated with apoptosis was analyzed by immunohistochemistry technique.
Results : Preventive Moxibustion Group (PM) and Ischemic preconditioning Group (IP) was notable diversity compare with Purely Ischemia-reperfusion group in every term of index. Preventive Moxibustion Group (PM) and Ischemic preconditioning Group (IP) were notable diversity compare with Sham Operating Group and Control Group. Preventive Moxibustion Group (PM) and Ischemic preconditioning Group (IP) was not notable diversity.
Conclusion: Preventive moxibustion have effects on cardiac muscle in myocardial ischemia rats, Preventive moxibusion can promote expression of heat shock protein 70, and Preventive moxibusion can induce the tolerance of heart muscle ischemia as a pretreatment method and relieve the impairment of cardial muscle after ischemic reperfusion.
方法:采用在体鼠心肌缺血再灌注损伤模型。将30只健康雄性Wistar大鼠,随机分成5组:缺血再灌注组——0号医用缝合线结扎冠状动脉左前降支,缺血20分钟,再灌注40分钟;缺血预处理组——缺血5分钟,再灌注5分钟,反复三次,后同上组;逆灸组——缺血再灌注前七天,艾灸“内关”“膻中”,每天2次,每次30分钟,后同上组;假手术组——手术仅以0号医用缝合线绕过冠状动脉左前降支,不予结扎;正常对照组——正常饲养。HE染色观察组织形态学变化。光镜观察心肌组织超微结构,免疫组织化学法检测各组心肌HSP70的含量表达。
结果:逆灸组(PM)、缺血预处理组(IP)HSP70表达水平明显增高,与缺血再灌注组(IR)相比有显著性差异(P＜0.05);预处理组(逆灸、缺血)与正常对照组及假手术组相比,各项指标有显著性差异(P＜0.05);逆灸组(PM)与缺血预处理组(IP)比较,各项指标无明显差异(P＞0.05)。
结论:逆灸对心肌缺血再灌注损伤大鼠心肌具有保护作用;逆灸可以增加HSP70的表达;逆灸作为一种心肌缺血的预处理手段,可以诱导心肌缺血耐受,减轻心肌缺血再灌注对心肌造成的损伤。
Objective: To observe the effects of preventive moxibustion on "Nei Guan"and "Tan Zhong" on expression of heat shock protein 70 and on the general structure histomorphology, to reveal the protective mechanism of preventive moxibustion on rat myocardial ischemia-reperfusion injury, then provide scientific experiment evidence.
Method: Using rat ischemia-reperfusion injury model in vivo, thirty male Wistar rats were randomly divided into five groups .Purely Ischemia-reperfusion group: the chest was opened and left anterior coronary was occluded by suture silk ,the occlusion was maintained for 20 minutes and released with reperfusion for 40 minutes ;Ischemic preconditioning Group (IP) : prior to ischemic reperfusion ,three cycles of 5 minutes of myocardial ischemia follow by 5 minutes of myocardial reperfusion , after which the procedures were the same to ditto; Preventive moxibustion Group(PM): 7 days prior to ischemic reperfusion ,moxibustion was given on "Nei Guan" "Tan Zhong" ,30 minutes twice daily .After which the procedures were the same to ditto .Sham Operating Group : the chest was opened and left anterior coronary was exposed with no further procedures; Control Group: Cadiocyte HE staining to observe change of histomorphology, Ultra structural pathological changes were observed with light microscope and the expression of heat shock protein 70 associated with apoptosis was analyzed by immunohistochemistry technique.
Results : Preventive Moxibustion Group (PM) and Ischemic preconditioning Group (IP) was notable diversity compare with Purely Ischemia-reperfusion group in every term of index. Preventive Moxibustion Group (PM) and Ischemic preconditioning Group (IP) were notable diversity compare with Sham Operating Group and Control Group. Preventive Moxibustion Group (PM) and Ischemic preconditioning Group (IP) was not notable diversity.
Conclusion: Preventive moxibustion have effects on cardiac muscle in myocardial ischemia rats, Preventive moxibusion can promote expression of heat shock protein 70, and Preventive moxibusion can induce the tolerance of heart muscle ischemia as a pretreatment method and relieve the impairment of cardial muscle after ischemic reperfusion.
引文
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