盆腔炎颗粒治疗慢性盆腔炎的疗效观察及对模型大鼠细胞凋亡影响的研究
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摘要
目的:通过观察盆腔炎颗粒治疗血瘀肾虚型慢性盆腔炎的临床疗效及对慢性盆腔炎模型大鼠细胞凋亡的变化,探讨盆腔炎颗粒治疗慢性盆腔炎(CPID)的细胞凋亡机制,为临床应用提供依据。
方法:将60例慢性盆腔炎患者随机分为治疗组(服用盆腔炎颗粒)、对照组(服用盆炎净颗粒),观察治疗前后临床症状、体征的改善情况。以Wistar雌性大鼠为实验对象,采用混合细菌加机械损伤方法,制备慢性盆腔炎动物模型。将60只大鼠随机分为7组,药物干预后,免疫组化S-P法检测各组大鼠子宫组织Caspase-3的表达;逆转录-聚合酶链反应法(RT-PCR)检测大鼠子宫组织Fas/FasLmRNA和p53mRNA的表达。
结果:临床观察结果显示,治疗组与对照组综合临床疗效的愈显率、总有效率比较有极显著性差异(P<0.01),治疗组疗效优于对照组;两组的中医证候疗效的愈显率、总有效率比较,均有显著性差异(P<0.05),治疗组疗效优于对照组。治疗后两组患者的综合积分、中医症状积分、局部体征积分比较均有显著性差异(P<0.05),治疗组明显优于对照组。慢性盆腔炎模型大鼠子宫组织中Caspase-3、Fas/FasLmRNA和p53mRNA的表达较正常组大鼠升高,差异均有极显著性(P<0.01)。药物干预后,。各治疗组大鼠子宫组织中Caspase-3、Fas/FasLmRNA和p53mRNA的表达与模型组相比较均增强(P<0.05)。除Caspase-3外,盆腔炎颗粒大剂量组的作用均明显优于盆炎净颗粒组(P<0.01)。盆腔炎颗粒各剂量组子宫组织FasmRNA及FasLmRNA表达相关系数为0.894,呈显著正相关性(P<0.01)。
结论:盆腔炎颗粒治疗血瘀肾虚型慢性盆腔炎有较好的临床疗效,通过增强慢性盆腔炎模型大鼠子宫组织中相关凋亡指标的表达,诱导炎细胞凋亡,抑制炎细胞生长,从而减轻炎症反应。
Objective: Through the observation pelvic inflammatory disease pellet treatment blood stasis concurrently kidney empty chronic pelvic inflammatory disease's clinical curative effect and the change which perishes weakly to the chronic pelvic inflammatory disease model big mouse cell, discusses the pelvic inflammatory disease pellet treatment chronic pelvic inflammatory disease (CPID) the cell perishes weakly the mechanism, provides the basis for the clinical practice.
Method: 60 cases of chronic pelvic inflammatory disease were divided into two groups randomly: Pen Qiangyan Granule treatment group and Pen Yanjing Granule control group, each group of 30 examples. Female Wistar rats were used and the CPID model was established with mixed bacteria injection combined with mechanic injury. Sixty rats were randomly divided into seven groups. After the histomorphological changes of the rats' uteri were observed, Immunity set the expression that turn a S-P law to examine each big rat womb organization Caspase-3;Converse to record-the polymerization Mao chain reaction the law(RT-PCR) examine the big rat womb organization Fas/FasLmRNA and the expression of the p 53 mRNAs.
Result: The clinical observation result suggests that more showing of the treatment set and matched control comprehensive clinical curative effect rate, always efficient relatively has polar show Zhao discrepancy(P<0.01), treatment set curative effect better than matched control;Two sets of Chinese medicine certificates wait more showing of the curative effect rate, always efficient compare, all have already shown Zhao discrepancy(P<0.05), the treatment set curative effect surpasses a matched control.After curing two sets of sufferers comprehensive integral, Chinese medicine symptom integral, partial body's advertising for integral a little bit all has already shown Zhao discrepancy(P<0.05), the treatment set obviously surpasses a matched control. Chronic basin chamber fire pattern big rat womb in the middle of the organization the expression of Caspase-3, Fas/FasLmRNA and the p 53 mRNAs more normal matched control big rat scale - up, the discrepancy all has very show Zhao.(P<0.01) After medicine interferes, each treatment set big rat womb organization amid expression and pattern set vis - a - vis of Caspase-3, Fas/FasLmRNA and the p 53 mRNAs all enhancement.(P<0.05)In addition to Caspase-3, the basin chamber blazes all obviously better than basin of the action of grain big amount of agent set burning net set.(P<0.01)Basin chamber burning each amount of agent of grain set womb organization FasmRNA and FasLmRNA voice a coefficient of correlation to 0.894 and present to show the Zhao positive correlation.(P<0.01).
Conclusions: Basin chamber fire the grain treatment blood Yu and kidney falsely the type chronic basin chamber fire contain better clinical curative effect and pass to strengthen chronic basin a chamber fire pattern big rat womb in the middle of the organization the expression of related beacon of Diao Wu, induce to blaze cell Diao Wu, repress to blaze a cell growth, ease to blaze disease reaction thus.
方法:将60例慢性盆腔炎患者随机分为治疗组(服用盆腔炎颗粒)、对照组(服用盆炎净颗粒),观察治疗前后临床症状、体征的改善情况。以Wistar雌性大鼠为实验对象,采用混合细菌加机械损伤方法,制备慢性盆腔炎动物模型。将60只大鼠随机分为7组,药物干预后,免疫组化S-P法检测各组大鼠子宫组织Caspase-3的表达;逆转录-聚合酶链反应法(RT-PCR)检测大鼠子宫组织Fas/FasLmRNA和p53mRNA的表达。
结果:临床观察结果显示,治疗组与对照组综合临床疗效的愈显率、总有效率比较有极显著性差异(P<0.01),治疗组疗效优于对照组;两组的中医证候疗效的愈显率、总有效率比较,均有显著性差异(P<0.05),治疗组疗效优于对照组。治疗后两组患者的综合积分、中医症状积分、局部体征积分比较均有显著性差异(P<0.05),治疗组明显优于对照组。慢性盆腔炎模型大鼠子宫组织中Caspase-3、Fas/FasLmRNA和p53mRNA的表达较正常组大鼠升高,差异均有极显著性(P<0.01)。药物干预后,。各治疗组大鼠子宫组织中Caspase-3、Fas/FasLmRNA和p53mRNA的表达与模型组相比较均增强(P<0.05)。除Caspase-3外,盆腔炎颗粒大剂量组的作用均明显优于盆炎净颗粒组(P<0.01)。盆腔炎颗粒各剂量组子宫组织FasmRNA及FasLmRNA表达相关系数为0.894,呈显著正相关性(P<0.01)。
结论:盆腔炎颗粒治疗血瘀肾虚型慢性盆腔炎有较好的临床疗效,通过增强慢性盆腔炎模型大鼠子宫组织中相关凋亡指标的表达,诱导炎细胞凋亡,抑制炎细胞生长,从而减轻炎症反应。
Objective: Through the observation pelvic inflammatory disease pellet treatment blood stasis concurrently kidney empty chronic pelvic inflammatory disease's clinical curative effect and the change which perishes weakly to the chronic pelvic inflammatory disease model big mouse cell, discusses the pelvic inflammatory disease pellet treatment chronic pelvic inflammatory disease (CPID) the cell perishes weakly the mechanism, provides the basis for the clinical practice.
Method: 60 cases of chronic pelvic inflammatory disease were divided into two groups randomly: Pen Qiangyan Granule treatment group and Pen Yanjing Granule control group, each group of 30 examples. Female Wistar rats were used and the CPID model was established with mixed bacteria injection combined with mechanic injury. Sixty rats were randomly divided into seven groups. After the histomorphological changes of the rats' uteri were observed, Immunity set the expression that turn a S-P law to examine each big rat womb organization Caspase-3;Converse to record-the polymerization Mao chain reaction the law(RT-PCR) examine the big rat womb organization Fas/FasLmRNA and the expression of the p 53 mRNAs.
Result: The clinical observation result suggests that more showing of the treatment set and matched control comprehensive clinical curative effect rate, always efficient relatively has polar show Zhao discrepancy(P<0.01), treatment set curative effect better than matched control;Two sets of Chinese medicine certificates wait more showing of the curative effect rate, always efficient compare, all have already shown Zhao discrepancy(P<0.05), the treatment set curative effect surpasses a matched control.After curing two sets of sufferers comprehensive integral, Chinese medicine symptom integral, partial body's advertising for integral a little bit all has already shown Zhao discrepancy(P<0.05), the treatment set obviously surpasses a matched control. Chronic basin chamber fire pattern big rat womb in the middle of the organization the expression of Caspase-3, Fas/FasLmRNA and the p 53 mRNAs more normal matched control big rat scale - up, the discrepancy all has very show Zhao.(P<0.01) After medicine interferes, each treatment set big rat womb organization amid expression and pattern set vis - a - vis of Caspase-3, Fas/FasLmRNA and the p 53 mRNAs all enhancement.(P<0.05)In addition to Caspase-3, the basin chamber blazes all obviously better than basin of the action of grain big amount of agent set burning net set.(P<0.01)Basin chamber burning each amount of agent of grain set womb organization FasmRNA and FasLmRNA voice a coefficient of correlation to 0.894 and present to show the Zhao positive correlation.(P<0.01).
Conclusions: Basin chamber fire the grain treatment blood Yu and kidney falsely the type chronic basin chamber fire contain better clinical curative effect and pass to strengthen chronic basin a chamber fire pattern big rat womb in the middle of the organization the expression of related beacon of Diao Wu, induce to blaze cell Diao Wu, repress to blaze a cell growth, ease to blaze disease reaction thus.
引文
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