人体坐骨神经内部显微结构三维可视化研究
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摘要
背景:
坐骨神经损伤后的治疗和康复是创伤外科的重点研究课题。坐骨神经损伤后,不论采用何种神经修复技术,基本目的均在于准确对合原先的神经束或功能相同的神经束,创造再生轴突抵达适宜末梢器官、恢复功能的最佳条件。但在临床实践中,神经损伤治疗的效果不甚理想,重要原因就是同一功能神经束的远近端不能准确缝接所致的运动或感觉神经纤维之间错向生长。因此构建坐骨神经内部神经束和束组的三维结构,了解其交叉重组的规律对治疗坐骨神经损伤具有重要临床意义。
目的:
按序提取并处理经乙酰胆碱酯酶染色的新鲜人尸坐骨神经连续组织切片的二维图像数字信息,通过3D-Nerve软件系统对坐骨神经内部显微结构进行三维可视化研究。
方法:
取自愿捐献尸体右侧坐骨神经1例,以头发为标记物,OCT包埋、液氮速冻固定后做连续组织切片,乙酰胆碱酯酶染色,高分辨率扫描仪依序获取坐骨神经二维图像信息,应用自行开发的3D-Nerve软件系统重建坐骨神经内部神经束的三维结构。
结果:
人体坐骨神经在不同断面神经束的数目、大小、形态及性质不断变化,在近侧断面神经束间频繁交叉重组,远侧断面的交叉重组较少。3D-Nerve软件可清晰显示坐骨神经各断面的二维显微结构,并能重建坐骨神经内部神经束及束组的三维立体图像,还可从不同角度观察神经束间的交叉重组情况。
结论:
通过3D-Nerve软件系统可较准确形象地显示人体坐骨神经任意断面的二维显微结构信息以及三维立体行径,提供了一种坐骨神经内部显微结构三维可视化的有效方法,为医学生及临床医师了解坐骨神经内部显微结构提供参考,对坐骨神经损伤后修复方式的选择有一定指导意义。
Background:The healing and rehabilitation after sciatic nerve injuries are one of the intensive topics in traumatic surgery.No matter what reparative technology is applied,the basic destination is to suture the original nerve-tracts,to restore identical function ones and to create the optimum conditions for regenerated axis cylinders reaching the peripheral organs and functional recovery.But in clinical practice,the effect is not quite ideal.The considerable agent is that the distal and proximal end of funiculi with identical functions can't be accurately sutured,which lead to irregular accretion in nerve fibres.So constructing the three -dimensional microstructure of nerve-tracts in sciatic nerves and comprehending the decussate and custom-crafted regularity have very important clinical significances.
Objective:The two-dimensional human sciatic nerve image information of series freezing tissue sections stained with Ache was acquired ordinally, then the three-dimensional visualization of the sciatic nerve was investigated with 3D-Nerve software systems.
Methods:One fresh sciatic nerve specimen was dissected,tagged with female hairs and embedded in OCT.Series freezing tissue sections were made,stained with AChe histochemically,acquired the two-dimensional imageinformation through high resolution scanner.Finally, three-dimensional microstructure was reconstructed with 3D-nerve software.
Results:The number,size,form and pattern of the sciatic nerve funiculi vary continually in different transverse sections.The funiculi unit divides frequently at some proximal transverse sections while there are rarely changes at the distal levels.The microstructure of the nerve funiculi is displayed distinctly at different transverse sections and three-dimensional images was reconstructed with 3D-nerve software system.In addition,we can observe them in different angles.
Conclusions:The two-dimensional and three-dimensional microstructure of the funiculi of human sciatic nerve were clearly reconstructed with 3D-nerve,which might provide an effective method for three-dimensional visualization of sciatic nerve and supply references to understand the microstructure of sciatic nerve for medical students and surgeons.It is also useful for the repairing process of sciatic nerve injury by providing the precise regional anatomic structure atlas of the nerve.
坐骨神经损伤后的治疗和康复是创伤外科的重点研究课题。坐骨神经损伤后,不论采用何种神经修复技术,基本目的均在于准确对合原先的神经束或功能相同的神经束,创造再生轴突抵达适宜末梢器官、恢复功能的最佳条件。但在临床实践中,神经损伤治疗的效果不甚理想,重要原因就是同一功能神经束的远近端不能准确缝接所致的运动或感觉神经纤维之间错向生长。因此构建坐骨神经内部神经束和束组的三维结构,了解其交叉重组的规律对治疗坐骨神经损伤具有重要临床意义。
目的:
按序提取并处理经乙酰胆碱酯酶染色的新鲜人尸坐骨神经连续组织切片的二维图像数字信息,通过3D-Nerve软件系统对坐骨神经内部显微结构进行三维可视化研究。
方法:
取自愿捐献尸体右侧坐骨神经1例,以头发为标记物,OCT包埋、液氮速冻固定后做连续组织切片,乙酰胆碱酯酶染色,高分辨率扫描仪依序获取坐骨神经二维图像信息,应用自行开发的3D-Nerve软件系统重建坐骨神经内部神经束的三维结构。
结果:
人体坐骨神经在不同断面神经束的数目、大小、形态及性质不断变化,在近侧断面神经束间频繁交叉重组,远侧断面的交叉重组较少。3D-Nerve软件可清晰显示坐骨神经各断面的二维显微结构,并能重建坐骨神经内部神经束及束组的三维立体图像,还可从不同角度观察神经束间的交叉重组情况。
结论:
通过3D-Nerve软件系统可较准确形象地显示人体坐骨神经任意断面的二维显微结构信息以及三维立体行径,提供了一种坐骨神经内部显微结构三维可视化的有效方法,为医学生及临床医师了解坐骨神经内部显微结构提供参考,对坐骨神经损伤后修复方式的选择有一定指导意义。
Background:The healing and rehabilitation after sciatic nerve injuries are one of the intensive topics in traumatic surgery.No matter what reparative technology is applied,the basic destination is to suture the original nerve-tracts,to restore identical function ones and to create the optimum conditions for regenerated axis cylinders reaching the peripheral organs and functional recovery.But in clinical practice,the effect is not quite ideal.The considerable agent is that the distal and proximal end of funiculi with identical functions can't be accurately sutured,which lead to irregular accretion in nerve fibres.So constructing the three -dimensional microstructure of nerve-tracts in sciatic nerves and comprehending the decussate and custom-crafted regularity have very important clinical significances.
Objective:The two-dimensional human sciatic nerve image information of series freezing tissue sections stained with Ache was acquired ordinally, then the three-dimensional visualization of the sciatic nerve was investigated with 3D-Nerve software systems.
Methods:One fresh sciatic nerve specimen was dissected,tagged with female hairs and embedded in OCT.Series freezing tissue sections were made,stained with AChe histochemically,acquired the two-dimensional imageinformation through high resolution scanner.Finally, three-dimensional microstructure was reconstructed with 3D-nerve software.
Results:The number,size,form and pattern of the sciatic nerve funiculi vary continually in different transverse sections.The funiculi unit divides frequently at some proximal transverse sections while there are rarely changes at the distal levels.The microstructure of the nerve funiculi is displayed distinctly at different transverse sections and three-dimensional images was reconstructed with 3D-nerve software system.In addition,we can observe them in different angles.
Conclusions:The two-dimensional and three-dimensional microstructure of the funiculi of human sciatic nerve were clearly reconstructed with 3D-nerve,which might provide an effective method for three-dimensional visualization of sciatic nerve and supply references to understand the microstructure of sciatic nerve for medical students and surgeons.It is also useful for the repairing process of sciatic nerve injury by providing the precise regional anatomic structure atlas of the nerve.
引文
1.陈菁,彭承琳,楚燕飞,等.周围神经损伤与再生的可视化研究.生物医学工程学杂志,2005;22(2):324-326.
2.戚剑,刘小林,熊卓,等.短段腓总神经功能束三维重建的初步研究.中国修复重建外科杂志,2008;22(9):1031-1035.
3.陈增淦,陈统一,张键,等.臂丛神经显微结构的计算机三维重建.中华骨科杂志,2004;24(8):462-466.
4.李智,张键,陈统一,等.人体桡神经连续组织切片计算机辅助三维重建研究.中国临床医学,2007;14(3):420-421.
5.谢小棉,陈思平,李树祥,等.尺神经功能束组三维可视化研究.中国生物医学工程学报,2004;23(3):199-204.
6.孙廓,胡平,张峰,等.人体正中神经内部显微结构的三维重建与可视化研究.中国矫形外科杂志,2008;16(18):1385-1388.
7.刘艇,胡平,张键,等.人体尺神经显微结构三维可视化研究.中国修复重建外科杂志,2008;22(9):1026-1030.
8.Karnoysky MJ,Root LA.Direteoloring thiocholine method for cholinesterase.J Histochem Cytochem,1964:12:219
9.王彦,孙桂媛,宋可饮.胆碱酯酶组化学技术在显微外科的应用.实用外科杂志,1987;7:252.
10.何蕴韶,钟世镇,乙酰胆碱酯酶组化在手术中鉴别神经束可能性的探讨.中国临床解剖学杂志,1987;5:13.
11.岳喜军,曹晓建,卜寿山.近红外光谱和聚类分析法快速鉴别神经束性质的初步研究.南京医科大学学报(自然科学版),2006;26(5):313-317.
12.王虎,路来金,孔祥贵等.微区喇曼光谱法快速鉴别周围神经束性质.中国临床康复,2006;10(8):104-106.
13.Merickel M.3D-reconstruction:The registration problem.Computer Version:Graphies Image Processing,1988;42(2):206-212.
14.Duerstock BS,Bajaj CL,Pascucci V,et al.Advances in three-dimensional reconstruction of the experimental spinal cord injury. Comput Med Imaging Graph,2000;24(6):389-406.
15.Salvi J,Matabosch C,Fofi D,et al.A review of recent range image registration methods with accuracy evaluation.Image and Vision Computing,2007;25(5):578-596.
16.Otten E,van Lecuwen JL.A 3-D reconstruction package from serial sections images.Eur J CellBiol,1989;48(Suppls 25):73
17.张显全,李自双,杨力等.肝血窦连续切片的计算机三维重建.生物医学工程学杂志,1997;14(2):195.
18.张景强,叶巧珍,赖德锦等.小白鼠肾脏线粒体三维结构的重构.中山大学学报(自然科学版),1990;129(3):73.
19.Carlbom I,Terzopoulos D,Harris M.Computer assisted registration segmentation and 3D reconstruction from images of neuronal tissue sections.IEEE Trans On Medi imaging,1994;13.
20.Sjostrand FS.J U Itrastrust.Res,1958;2:122.
21.Willey TJ.Computer graphics in three dimensions for prepective reconstruction of brain ultrastructure.IEEE Trans On Biomed Eng,July 1973;288
22.王今著.生物组织显微镜结构三维重建的灰度阴影立体图对显示技术.生物物理学报,1990;2(3):17
23.Heffeman PB,Robb RA.A new method of shaded surface display of biological and medical image.IEEE Trans on Medical Imaging,1985:MI-4:25
24.Udapa JK.Interactive segmentation and boundary surface formation for 3D digital images.Comput Graph Imag Proc,1982;18(15):213
25.张义,李时光,杨恬.生物组织显微切片图像计算机三维重建的截面重建技术.中国生物医学工程学报,1992;11(1):17
26.李淑梁,陈洁.三维CT图像的显示与重建.清华大学学报,1986;26(5):10
27.陈菁,等.多目标离散断层图像序列的三维重建算法研究.重庆大学硕士学位论文,1995
28.谢水生,李华,林宗楷等.基于连续组织切片的神经三维可视化研究.计算机应用研究,2007;24(6):170-172.
29.朱家恺.现代周围神经外科学.上海:上海科学技术出版社,2007:29-30.
30.朱家恺.现代周围神经外科学.上海:上海科学技术出版社,2007:485-491.
31.Terzis JK.神经修复的过去、现在与未来.第1版.济南:山东科学技术出版社,2000;354-364.
1.Chappell KE,Robson MD,Stonebridge-Foster A,et al.Magic angle effects in MR neurography.AJNR Am J Neuroradiol,2004,25(3):431-440.
2.任高宏,裴国献.虚拟技术对21世纪微创外科发展的影响.中华创伤骨科杂志,2004,6(8):899-902,909.
3.Pommert A,H(o|¨)hne KH,Burmester E,et al.Computer-based anatomy a prerequisite for computer-assisted radiology and surgery.Acad Radiol,2006,13(1):104-112.
4.Penkert G,Carvalho GA,Nikkhah G,et al.Diagnosis and surgery of brachial plexus injuries.J Reconstr Microsurg,1999,15(1):3-8.
5.刘光久,张绍祥,刘正津,等.首例中国数字化可视人体脊柱区颈段的三维重建研究.局解手术学杂志,2003,12(3):177-179.
6.张元智,顾立强,尹博,等.虚拟中国人女Ⅰ号(VCH-FⅠ)臂丛神经可视化初步研究.中华手外科杂志,2005,21(5):277-279.
7.刘光久,张绍祥,刘正津,等.臂丛神经的断层解剖观察与可视化研究.第三军医大学学报,2007,29(5):373-375.
8.彭田红,张元智,李严斌,等.上肢血管与神经的三维重建研究.中华手外科杂志,2007,23(3):180-182.
9.张元智,顾立强,原林,等.腰骶丛神经的断层解剖学及可视化初步研究.中华创伤骨科杂志,2004,6(12):1362-1364.
10.邱明国,张绍祥,刘正津,等.男性盆底可视化研究.第三军医大学学报,2004,26(4):328-331.
11.Jokisch DW,Patton PW,Rajon DA,et al.Chord distributions across 3D digital images of a human thoraci vertebra.Med Phys,2001,28(7):1493-1504.
12.张景僚,顾立强,王龙江,等.L4、5神经前支和腰骶干与骶髂关节关系的三维重建.南方医科大学学报,2006,26(3):364-366.
13.陆声,徐永清,张元智,等.腰丛神经的数字化解剖及其临床意义.解剖与临床,2007,12(4):233-235.
14.Sjostrand FS.J U Itrastrust.Res,1958:2:122.
15.Willey TJ.Computer graphics in three dimensions for prepective reconstruction of brain ultrastructure.IEEE Trans On Biomed Eng,July 1973:288
16.王今著.生物组织显微镜结构三维重建的灰度阴影立体图对显示技术.生物物理学报,1990;2(3):17
17.Heffeman PB,Robb RA.A new method of shaded surface display of biological and medical image.IEEE Trans on Medical Imaging,1985:MI-4:25
18.Udapa JK.Interactive segmentation and boundary surface formation for 3D digital images.Comput Graph Imag Proc,1982;18(15):213
19.张义,李时光,杨恬.生物组织显微切片图像计算机三维重建的截面重建技术.中国生物医学工程学报,1992;11(1):17
20.李淑梁,陈洁.三维CT图像的显示与重建.清华大学学报,1986;26(5):10
21.陈菁,等.多目标离散断层图像序列的三维重建算法研究.重庆大学硕士学位论文,1995
22.Watchemaker GP,Gumucio CA,Crandall RE,et al.Fascicular topograpy of the median nerve:A computer based to identify branching patterns.J Hand Surg(Am),1991:16(1):53-59.
23.陈增淦,陈统一,张键,等.臂丛神经显微结构的计算机三维重建.中华骨科杂志,2004;24(8):462-466.
24.李智,张键,陈统一,等.人体桡神经连续组织切片计算机辅助三维重建研究.中国临床医学,2007;14(3):420-421.
25.谢小棉,陈思平,李树祥,等.尺神经功能束组三维可视化研究.中国生物医学工程学报,2004;23(3):199-204.
26.孙廓,胡平,张峰,等.人体正中神经内部显微结构的三维重建与可视化研究。中国矫形外科杂志,2008;16(18):1385-1388.
27.刘艇,胡平,张键,等.人体尺神经显微结构三维可视化研究.中国修复重建 外科杂志,2008;22(9):1026-1030.
28.戚剑,刘小林,熊卓,等.短段腓总神经功能束三维重建的初步研究.中国修复重建外科杂志,2008;22(9):1031-1035.
29.陈菁,彭承琳,楚燕飞,等.周围神经损伤与再生的可视化研究.生物医学工程学杂志,2005;22(2):324-326.
30.Imel i(?)ska C,Dowries MS,Yuan W.Semi-automated color segmentation of anatomical tissue.Comput Med Imaging Graph,2000,24(3):173-180.
31.Schiemann T,Tiede U,H(o|¨)hne KH.Segmentation of the visible human for high-qualify volume-based visualization.Med Image Anal,1997,1(4):263-270.
32.Kuxhaus L,Roach SS,Valero-Cuevas FJ.Quantifying deficits in the 3D force capabilities of a digit caused by selective paralysis:application to the thumb with simulated low ulnar nerve palsy.J Biomech,2005,38(4):725-736.
33.Fodil R,Laurent V,Planus E,et al.Characterization of cytoskeleton mechanical properties and 3D-actin structure in twisted adherent epithel ial cells.Biorheology,2003,40(1-3):241-245.
34.Hang PA,Fung PF,Wong TT,et al.Virtual lwonchoscopy.Int J Virt Real,2000,4(4):10-20.
35.Hounnou GM,Uhl JF,Plaisant O,et al.Murphometry by computerized three-dimensional reconstruction of the hypogastric plexus of a human fetus.Surg Radiol Anat,2003,25(1):21-31.
2.戚剑,刘小林,熊卓,等.短段腓总神经功能束三维重建的初步研究.中国修复重建外科杂志,2008;22(9):1031-1035.
3.陈增淦,陈统一,张键,等.臂丛神经显微结构的计算机三维重建.中华骨科杂志,2004;24(8):462-466.
4.李智,张键,陈统一,等.人体桡神经连续组织切片计算机辅助三维重建研究.中国临床医学,2007;14(3):420-421.
5.谢小棉,陈思平,李树祥,等.尺神经功能束组三维可视化研究.中国生物医学工程学报,2004;23(3):199-204.
6.孙廓,胡平,张峰,等.人体正中神经内部显微结构的三维重建与可视化研究.中国矫形外科杂志,2008;16(18):1385-1388.
7.刘艇,胡平,张键,等.人体尺神经显微结构三维可视化研究.中国修复重建外科杂志,2008;22(9):1026-1030.
8.Karnoysky MJ,Root LA.Direteoloring thiocholine method for cholinesterase.J Histochem Cytochem,1964:12:219
9.王彦,孙桂媛,宋可饮.胆碱酯酶组化学技术在显微外科的应用.实用外科杂志,1987;7:252.
10.何蕴韶,钟世镇,乙酰胆碱酯酶组化在手术中鉴别神经束可能性的探讨.中国临床解剖学杂志,1987;5:13.
11.岳喜军,曹晓建,卜寿山.近红外光谱和聚类分析法快速鉴别神经束性质的初步研究.南京医科大学学报(自然科学版),2006;26(5):313-317.
12.王虎,路来金,孔祥贵等.微区喇曼光谱法快速鉴别周围神经束性质.中国临床康复,2006;10(8):104-106.
13.Merickel M.3D-reconstruction:The registration problem.Computer Version:Graphies Image Processing,1988;42(2):206-212.
14.Duerstock BS,Bajaj CL,Pascucci V,et al.Advances in three-dimensional reconstruction of the experimental spinal cord injury. Comput Med Imaging Graph,2000;24(6):389-406.
15.Salvi J,Matabosch C,Fofi D,et al.A review of recent range image registration methods with accuracy evaluation.Image and Vision Computing,2007;25(5):578-596.
16.Otten E,van Lecuwen JL.A 3-D reconstruction package from serial sections images.Eur J CellBiol,1989;48(Suppls 25):73
17.张显全,李自双,杨力等.肝血窦连续切片的计算机三维重建.生物医学工程学杂志,1997;14(2):195.
18.张景强,叶巧珍,赖德锦等.小白鼠肾脏线粒体三维结构的重构.中山大学学报(自然科学版),1990;129(3):73.
19.Carlbom I,Terzopoulos D,Harris M.Computer assisted registration segmentation and 3D reconstruction from images of neuronal tissue sections.IEEE Trans On Medi imaging,1994;13.
20.Sjostrand FS.J U Itrastrust.Res,1958;2:122.
21.Willey TJ.Computer graphics in three dimensions for prepective reconstruction of brain ultrastructure.IEEE Trans On Biomed Eng,July 1973;288
22.王今著.生物组织显微镜结构三维重建的灰度阴影立体图对显示技术.生物物理学报,1990;2(3):17
23.Heffeman PB,Robb RA.A new method of shaded surface display of biological and medical image.IEEE Trans on Medical Imaging,1985:MI-4:25
24.Udapa JK.Interactive segmentation and boundary surface formation for 3D digital images.Comput Graph Imag Proc,1982;18(15):213
25.张义,李时光,杨恬.生物组织显微切片图像计算机三维重建的截面重建技术.中国生物医学工程学报,1992;11(1):17
26.李淑梁,陈洁.三维CT图像的显示与重建.清华大学学报,1986;26(5):10
27.陈菁,等.多目标离散断层图像序列的三维重建算法研究.重庆大学硕士学位论文,1995
28.谢水生,李华,林宗楷等.基于连续组织切片的神经三维可视化研究.计算机应用研究,2007;24(6):170-172.
29.朱家恺.现代周围神经外科学.上海:上海科学技术出版社,2007:29-30.
30.朱家恺.现代周围神经外科学.上海:上海科学技术出版社,2007:485-491.
31.Terzis JK.神经修复的过去、现在与未来.第1版.济南:山东科学技术出版社,2000;354-364.
1.Chappell KE,Robson MD,Stonebridge-Foster A,et al.Magic angle effects in MR neurography.AJNR Am J Neuroradiol,2004,25(3):431-440.
2.任高宏,裴国献.虚拟技术对21世纪微创外科发展的影响.中华创伤骨科杂志,2004,6(8):899-902,909.
3.Pommert A,H(o|¨)hne KH,Burmester E,et al.Computer-based anatomy a prerequisite for computer-assisted radiology and surgery.Acad Radiol,2006,13(1):104-112.
4.Penkert G,Carvalho GA,Nikkhah G,et al.Diagnosis and surgery of brachial plexus injuries.J Reconstr Microsurg,1999,15(1):3-8.
5.刘光久,张绍祥,刘正津,等.首例中国数字化可视人体脊柱区颈段的三维重建研究.局解手术学杂志,2003,12(3):177-179.
6.张元智,顾立强,尹博,等.虚拟中国人女Ⅰ号(VCH-FⅠ)臂丛神经可视化初步研究.中华手外科杂志,2005,21(5):277-279.
7.刘光久,张绍祥,刘正津,等.臂丛神经的断层解剖观察与可视化研究.第三军医大学学报,2007,29(5):373-375.
8.彭田红,张元智,李严斌,等.上肢血管与神经的三维重建研究.中华手外科杂志,2007,23(3):180-182.
9.张元智,顾立强,原林,等.腰骶丛神经的断层解剖学及可视化初步研究.中华创伤骨科杂志,2004,6(12):1362-1364.
10.邱明国,张绍祥,刘正津,等.男性盆底可视化研究.第三军医大学学报,2004,26(4):328-331.
11.Jokisch DW,Patton PW,Rajon DA,et al.Chord distributions across 3D digital images of a human thoraci vertebra.Med Phys,2001,28(7):1493-1504.
12.张景僚,顾立强,王龙江,等.L4、5神经前支和腰骶干与骶髂关节关系的三维重建.南方医科大学学报,2006,26(3):364-366.
13.陆声,徐永清,张元智,等.腰丛神经的数字化解剖及其临床意义.解剖与临床,2007,12(4):233-235.
14.Sjostrand FS.J U Itrastrust.Res,1958:2:122.
15.Willey TJ.Computer graphics in three dimensions for prepective reconstruction of brain ultrastructure.IEEE Trans On Biomed Eng,July 1973:288
16.王今著.生物组织显微镜结构三维重建的灰度阴影立体图对显示技术.生物物理学报,1990;2(3):17
17.Heffeman PB,Robb RA.A new method of shaded surface display of biological and medical image.IEEE Trans on Medical Imaging,1985:MI-4:25
18.Udapa JK.Interactive segmentation and boundary surface formation for 3D digital images.Comput Graph Imag Proc,1982;18(15):213
19.张义,李时光,杨恬.生物组织显微切片图像计算机三维重建的截面重建技术.中国生物医学工程学报,1992;11(1):17
20.李淑梁,陈洁.三维CT图像的显示与重建.清华大学学报,1986;26(5):10
21.陈菁,等.多目标离散断层图像序列的三维重建算法研究.重庆大学硕士学位论文,1995
22.Watchemaker GP,Gumucio CA,Crandall RE,et al.Fascicular topograpy of the median nerve:A computer based to identify branching patterns.J Hand Surg(Am),1991:16(1):53-59.
23.陈增淦,陈统一,张键,等.臂丛神经显微结构的计算机三维重建.中华骨科杂志,2004;24(8):462-466.
24.李智,张键,陈统一,等.人体桡神经连续组织切片计算机辅助三维重建研究.中国临床医学,2007;14(3):420-421.
25.谢小棉,陈思平,李树祥,等.尺神经功能束组三维可视化研究.中国生物医学工程学报,2004;23(3):199-204.
26.孙廓,胡平,张峰,等.人体正中神经内部显微结构的三维重建与可视化研究。中国矫形外科杂志,2008;16(18):1385-1388.
27.刘艇,胡平,张键,等.人体尺神经显微结构三维可视化研究.中国修复重建 外科杂志,2008;22(9):1026-1030.
28.戚剑,刘小林,熊卓,等.短段腓总神经功能束三维重建的初步研究.中国修复重建外科杂志,2008;22(9):1031-1035.
29.陈菁,彭承琳,楚燕飞,等.周围神经损伤与再生的可视化研究.生物医学工程学杂志,2005;22(2):324-326.
30.Imel i(?)ska C,Dowries MS,Yuan W.Semi-automated color segmentation of anatomical tissue.Comput Med Imaging Graph,2000,24(3):173-180.
31.Schiemann T,Tiede U,H(o|¨)hne KH.Segmentation of the visible human for high-qualify volume-based visualization.Med Image Anal,1997,1(4):263-270.
32.Kuxhaus L,Roach SS,Valero-Cuevas FJ.Quantifying deficits in the 3D force capabilities of a digit caused by selective paralysis:application to the thumb with simulated low ulnar nerve palsy.J Biomech,2005,38(4):725-736.
33.Fodil R,Laurent V,Planus E,et al.Characterization of cytoskeleton mechanical properties and 3D-actin structure in twisted adherent epithel ial cells.Biorheology,2003,40(1-3):241-245.
34.Hang PA,Fung PF,Wong TT,et al.Virtual lwonchoscopy.Int J Virt Real,2000,4(4):10-20.
35.Hounnou GM,Uhl JF,Plaisant O,et al.Murphometry by computerized three-dimensional reconstruction of the hypogastric plexus of a human fetus.Surg Radiol Anat,2003,25(1):21-31.