自身免疫性甲状腺疾病患者血清和发硒含量的研究
摘要
目的:检测自身免疫性甲状腺疾病(AITD)患者(GD、HT)与正常对照人群血清、头发硒(Se)含量,探讨Se与自身免疫性甲状腺疾病之间的关系。
方法:研究对象分对照组(n=43),AITD组(n=112);AITD组分GD组(n=73)和HT组(n=39)亚组。采用放射免疫(RtA)法统一检测甲状腺过氧化酶抗体(TMAb).和甲状腺球蛋白抗体(TGAb)。入组人员抽取空腹静脉血,用湿法消化血清、头发,采用氢化物原子荧光法检测血清、头发硒,采用SPSS16.0统计软件对检测数据进行统计学处理,p＜0.05有统计学意义。
结果:①AITD组血清、头发硒含量低于正常对照组,p＜0.05;GD及HT组间血清硒、头发硒含量浓度无显著性差异,p＞0.05。②血清、头发硒与甲状腺肿大程度呈负相关,随甲状腺肿大程度加重,血清与头发硒含量逐渐下降,Ⅲ度肿大者血清与发硒含量低于无肿大者,p＜0.05。③血清和发硒含量与TMAb、TGAb呈负相关,p＜0.05。④血清硒水平与头发硒含量呈正相关,p＜0.05。
结论:GD和HT患者血清硒水平和头发硒含量均明显降低。血清硒水平和头发硒含量两者密切相关,联合监测有助于准确评价AITD患者体内低硒状态。血清硒水平和头发硒含量与AITD患者自身甲状腺免疫抗体呈显著负相关,提示低硒状态与自身免疫性甲状腺疾病发生有关。
Objective To detect the selenium(Se) in serum and hair of patients with autoimmune thyroid diseases(AITD),included Graves(GD),Hashimoto thyroiditis(HT) compared with normal controls.To investigate the relationship between Se and autoimmune thyroid diseases.
Methods The subjects were divided into control group(n=43) and AITD group(n=112 ). Then the AITD group was subdivided into GD group(n=73) and HT group(n=39).All the subjects were detected TMAb and TGAb.Every subject was drew fasting venous blood,the detection including TGAb,TMAb,selenium in the serum and hair.The methods of digestion hair and serum were wet methods,then applying the method of hydride generation atomic fluorescence spectrumetry to detect the Se in serum and hair.
Results①The levels of the selenium in the serum and the hair of patients with the AITD is lower than the control group,p＜0.05;there is no significant difference between the GD group and HT group,p＞0.05.②The correlation between the goiter and the level of selenium in the serum and the hair is negative;The correlation between the level of selenium in the serum and hair with theⅢ°goiter is significant,p＜0.05.③The correlation between the level of selenium in the serum and the hair with the level of TMAb and TGAb is negative,p＜0.05.④The correlation between the level of selenium in the serum and the hair is positive,p＜0.05.
Conclusion:The level of selenium in the serum and hair of GD patients is significant lower than the controls,so is the HT patients;The level of selenium in the serum is closely related to the selenium in the hair.The joint monitoring can accurate evaluate the patients with AITD in lower selenium status.The negative correlation between the level of selenium in the serum and the hair with the level of TMAb and TGAb show that the lower selenium status is related to the AITD.
方法:研究对象分对照组(n=43),AITD组(n=112);AITD组分GD组(n=73)和HT组(n=39)亚组。采用放射免疫(RtA)法统一检测甲状腺过氧化酶抗体(TMAb).和甲状腺球蛋白抗体(TGAb)。入组人员抽取空腹静脉血,用湿法消化血清、头发,采用氢化物原子荧光法检测血清、头发硒,采用SPSS16.0统计软件对检测数据进行统计学处理,p＜0.05有统计学意义。
结果:①AITD组血清、头发硒含量低于正常对照组,p＜0.05;GD及HT组间血清硒、头发硒含量浓度无显著性差异,p＞0.05。②血清、头发硒与甲状腺肿大程度呈负相关,随甲状腺肿大程度加重,血清与头发硒含量逐渐下降,Ⅲ度肿大者血清与发硒含量低于无肿大者,p＜0.05。③血清和发硒含量与TMAb、TGAb呈负相关,p＜0.05。④血清硒水平与头发硒含量呈正相关,p＜0.05。
结论:GD和HT患者血清硒水平和头发硒含量均明显降低。血清硒水平和头发硒含量两者密切相关,联合监测有助于准确评价AITD患者体内低硒状态。血清硒水平和头发硒含量与AITD患者自身甲状腺免疫抗体呈显著负相关,提示低硒状态与自身免疫性甲状腺疾病发生有关。
Objective To detect the selenium(Se) in serum and hair of patients with autoimmune thyroid diseases(AITD),included Graves(GD),Hashimoto thyroiditis(HT) compared with normal controls.To investigate the relationship between Se and autoimmune thyroid diseases.
Methods The subjects were divided into control group(n=43) and AITD group(n=112 ). Then the AITD group was subdivided into GD group(n=73) and HT group(n=39).All the subjects were detected TMAb and TGAb.Every subject was drew fasting venous blood,the detection including TGAb,TMAb,selenium in the serum and hair.The methods of digestion hair and serum were wet methods,then applying the method of hydride generation atomic fluorescence spectrumetry to detect the Se in serum and hair.
Results①The levels of the selenium in the serum and the hair of patients with the AITD is lower than the control group,p＜0.05;there is no significant difference between the GD group and HT group,p＞0.05.②The correlation between the goiter and the level of selenium in the serum and the hair is negative;The correlation between the level of selenium in the serum and hair with theⅢ°goiter is significant,p＜0.05.③The correlation between the level of selenium in the serum and the hair with the level of TMAb and TGAb is negative,p＜0.05.④The correlation between the level of selenium in the serum and the hair is positive,p＜0.05.
Conclusion:The level of selenium in the serum and hair of GD patients is significant lower than the controls,so is the HT patients;The level of selenium in the serum is closely related to the selenium in the hair.The joint monitoring can accurate evaluate the patients with AITD in lower selenium status.The negative correlation between the level of selenium in the serum and the hair with the level of TMAb and TGAb show that the lower selenium status is related to the AITD.
引文
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3 Guangqi Yang,shuzhen Wang.Endemic selenium intoxication of humans in China.American Journal of clinical Nutrition 37:MAY 1983,pp 872-881.Printed in USA
4 佟雅洁,,滕卫平.不同碘摄入量地区硒与甲状腺功能关系的流行病学调查.中华内分泌学杂志2003年12月10日第83卷第23期164-166
5 迟海燕,阎胜利,余霄龙,等.亚硒酸钠对大鼠自身免疫性甲状腺炎影响的研究.2007 Vo17,No2.205 113-115
6 张方华,阎胜利,等.亚硒酸钠对体外培养人甲状腺细胞氧化损伤的影响.免疫学杂地 2008242,139-141
7 GUO H,YANG X,XU J,Effect of selenium on thyroid hormone metabolism in filial cerebrum of mice with excessive iodine exposure.Biol Trace-Elem Res.2006 Dec:113(3)281-95.
8 YANG XF,HOUXH,XU J.Effect of selenium supplementation on activity and mRNA expression of type 1 deiodinase in mice with excessive iodine intake.Biomed Environ Sci.2006Aug:19(4):302-8.
9 Turker,K.Kumanlioglu,I.Karapolat,and I.Dogan Selenium treatment in autoimmune thyroidditis:9-month follow-up with variable doses.J.Endocrinol.July 1,2006;190(1):151 - 156.
10 Gartner R,Gasnier BC.Selenium in the treatment of autoimmune thyroiditis.Biofactors,2003,19:165-170.
11 Broome CS,Mc Ardle F,Kyle JA,Andrews F,Lowe NM,Hart CA,Arthur JR & Jackson MJ.An increase in selenium intake improves immune function and poliovirus handling in adults with marginal selenium status.American Journal of Clinical Nutrition 200480 154- 162.
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13 Thomson CD,Mclachlan SK,The effect of selenium on thyroid status in a population with marginal selenium and iodine status.Br J Nutr.2005 Dec:94(6):962-968.
14 Berger M M,Reymond M J,Shenkin A,et al.Influence of selenium supplements on the postraumatic alterations of the thyroidaxis;a placebo-controlled trial[J]Intensive Care Med,20-01,27:91-100.
15 XU JIN,HOU XF,Selenium supplement alleviated the toxic effects of excessive iodine in mice.Biol Trace Elem Res.2006 Summer;111(1-3):229-238.
16 Kucharzewski M,Braziewicz J,Majewska L,etal.Concentration of selenium in the whole blood and the thyroid tissue of patients with various thyroid diseases[J].Biol Trace Elem Res,2000,88(1):25-30.
17 Mazokopakis EE,Papadakis JA,Effects of 12 months treatment with L-selenomethionine on serum anti-TPO Levels in Patients with Hashimotos thyroiditis.Thyroid.2007 Jul;17(7):609-612
18 Contempre B,Dumont JE,Denef J-F,Many MC,Effects of selenium deficiency on thyroid necrosis,fibrosis and proliferations possible role in myxoedematous cretinism.Eur J Endocrinol 1995 133:99-109
19 Arnaud J,Malvy D,Richard M J,etal.Selenium status in an iodine deficient population of the West Ivory Coast[J]Physiol Anthropol Appl Human Sci,2001,20(2):81-84
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2 赵霖,鲍善芬.青少年近视患者发硒水平的观察.J Nucl Med 1987 13 187-191
3 Guangqi Yang,shuzhen Wang.Endemic selenium intoxication of humans in China.American Journal of clinical Nutrition 37:MAY 1983,pp 872-881.Printed in USA
4 佟雅洁,,滕卫平.不同碘摄入量地区硒与甲状腺功能关系的流行病学调查.中华内分泌学杂志2003年12月10日第83卷第23期164-166
5 迟海燕,阎胜利,余霄龙,等.亚硒酸钠对大鼠自身免疫性甲状腺炎影响的研究.2007 Vo17,No2.205 113-115
6 张方华,阎胜利,等.亚硒酸钠对体外培养人甲状腺细胞氧化损伤的影响.免疫学杂地 2008242,139-141
7 GUO H,YANG X,XU J,Effect of selenium on thyroid hormone metabolism in filial cerebrum of mice with excessive iodine exposure.Biol Trace-Elem Res.2006 Dec:113(3)281-95.
8 YANG XF,HOUXH,XU J.Effect of selenium supplementation on activity and mRNA expression of type 1 deiodinase in mice with excessive iodine intake.Biomed Environ Sci.2006Aug:19(4):302-8.
9 Turker,K.Kumanlioglu,I.Karapolat,and I.Dogan Selenium treatment in autoimmune thyroidditis:9-month follow-up with variable doses.J.Endocrinol.July 1,2006;190(1):151 - 156.
10 Gartner R,Gasnier BC.Selenium in the treatment of autoimmune thyroiditis.Biofactors,2003,19:165-170.
11 Broome CS,Mc Ardle F,Kyle JA,Andrews F,Lowe NM,Hart CA,Arthur JR & Jackson MJ.An increase in selenium intake improves immune function and poliovirus handling in adults with marginal selenium status.American Journal of Clinical Nutrition 200480 154- 162.
12 Y.Song,N.Driessens,M.Costa,X.De Deken,V.Detours,B.Corvilain,C.Maenhaut,F.Miot,J.Van Sande,MC.Many,etal.Roles of Hydrogen Peroxide in Thyroid Physiology and Disease J.Clin.Endocrinol.Metab.October 1,2007;92(10):3764 - 3773.
13 Thomson CD,Mclachlan SK,The effect of selenium on thyroid status in a population with marginal selenium and iodine status.Br J Nutr.2005 Dec:94(6):962-968.
14 Berger M M,Reymond M J,Shenkin A,et al.Influence of selenium supplements on the postraumatic alterations of the thyroidaxis;a placebo-controlled trial[J]Intensive Care Med,20-01,27:91-100.
15 XU JIN,HOU XF,Selenium supplement alleviated the toxic effects of excessive iodine in mice.Biol Trace Elem Res.2006 Summer;111(1-3):229-238.
16 Kucharzewski M,Braziewicz J,Majewska L,etal.Concentration of selenium in the whole blood and the thyroid tissue of patients with various thyroid diseases[J].Biol Trace Elem Res,2000,88(1):25-30.
17 Mazokopakis EE,Papadakis JA,Effects of 12 months treatment with L-selenomethionine on serum anti-TPO Levels in Patients with Hashimotos thyroiditis.Thyroid.2007 Jul;17(7):609-612
18 Contempre B,Dumont JE,Denef J-F,Many MC,Effects of selenium deficiency on thyroid necrosis,fibrosis and proliferations possible role in myxoedematous cretinism.Eur J Endocrinol 1995 133:99-109
19 Arnaud J,Malvy D,Richard M J,etal.Selenium status in an iodine deficient population of the West Ivory Coast[J]Physiol Anthropol Appl Human Sci,2001,20(2):81-84
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