外源Ca~(2+)处理下杨树扦插苗光合同化物的分配格局
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本论文以南林895扦插苗为试验材料,采用形态指标检测、生理生化测定、稳定同位素分析等技术,研究了外源Ca~(2+)处理下南林895杨扦插苗的营养生长状况,探讨了外源Ca~(2+)对南林895杨扦插苗光合同化物分配格局的响应,结果表明:
     1.外源Ca~(2+)能够促进南林895杨扦插苗的营养生长。喷施外源Ca~(2+)后,扦插苗的株高、地径、生物量均优于对照,其中以200mg/L浓度水平的Ca~(2+)处理效果最好;2、20mg/L Ca~(2+)对扦插苗的促进效应不明显,300mg/L Ca~(2+)浓度对扦插苗的促进效应相对较弱。
     2.外源Ca~(2+)可以改善南林895杨扦插苗光合作用效率。不同浓度Ca~(2+)处理下扦插苗净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、细胞间CO2浓度(Ci)均呈现先升高后下降的趋势。随着Ca~(2+)浓度的升高,20mg/L Ca~(2+)处理下Ci值最大;但Pn、Tr、Gs在200mg/L Ca~(2+)处理下才达到最大值,与对照差异性显著。
     3.外源Ca~(2+)可以提高南林895杨扦插苗叶绿素、可溶性蛋白质的含量。较高浓度的Ca~(2+)(100~300mg/L)对植株的影响作用较大,在处理20d前,以200mg/L Ca~(2+)处理效果最佳;25d后以100mg/L Ca~(2+)处理效果最好。
     4.外源Ca~(2+)可以调控南林895杨扦插苗可溶性糖含量及其糖代谢相关酶活性的动态变化。“源”器官可溶性糖含量及代谢相关酶活性高于“库”器官,推测外源Ca~(2+)提高了南林895杨扦插苗“库”(茎)的强度,促进其光合同化物由“源”(叶)向“库”中转运。
     5.外源Ca~(2+)影响南林895杨扦插苗营养器官对稳定同位素碳、氮的富集能力。随着Ca~(2+)浓度的升高,δ13C、δ15N值均呈现逐渐上升然后缓慢下降的趋势。δ13C、δ15N值在植株中的分布遵从“库”器官大于“源”器官的规律。
     6.外源Ca~(2+)对南林895杨扦插苗生长的促进作用具有一定的累积效应。低浓度Ca~(2+)随着时间的延长而作用效果加强,而高浓度Ca~(2+)随着生长期的延长其作用效果逐渐减弱。
Using the methods of morphological index measuring, physiological and biochemical testing, stable isotope analysis techniques, we investigated the dynamic changes of regularity on vegetative growth status of Populus×euramericana‘Nanlin 895’cuttings under different concentration of exogenous Ca~(2+) treatment. And that we also explored the effects of exogenous Ca~(2+) on photo-assimilate allocation patterns of Populus×euramericana‘Nanlin 895’cuttings. The results showed as follows:
     1. The exogenous Ca~(2+)was able to accelerate the vegetative growth of Populus×Eurameri cana‘Nanlin 895’cuttings. The plant height, caliper, bio-yield were superior to control test after spraying different concentration of exogenous Ca~(2+). Thereinto, the 200mg/L Ca~(2+)concentration treatment was the best one especially. The 2, 20mg/L Ca~(2+)concentrations treatment had a slightly positive effect which was compared with the control test. And the enhance effect would be decreased if the Ca~(2+) concentration reached to 300mg/L.
     2. The exogenous Ca~(2+)could promote the photosynthesis efficiency of Populus×eurameri cana‘Nanlin 895’cuttings. With the Ca~(2+) concentration increasing, the Pn、Tr、Gs、Ci of leaves were presented gradually trends of increase in advance, and then decrease without exception. The value of Ci would reach to maximum when the Ca~(2+) concentration treatment was 20mg/L. However, the value of Pn、Tr、Gs would reach to the peak when the concentration exogenous Ca~(2+) concentration treatment was 200mg/L.
     3. The exogenous Ca~(2+) could increase the chlorophyll and soluble protein content of Populus×euramericana‘Nanlin 895’cuttings. And that the relative higher Ca~(2+) concentration presented an obvious stimulative effect. The 200mg/L Ca~(2+) was the optimal concentration before spraying 20 days, while the 100mg/L Ca~(2+) was the optimal concentration after 25 days.
     4. The exogenous Ca~(2+) could regulate the dynamic changes of soluble sugar content and sugar-metabolizing related-enzyme of Populus×euramericana‘Nanlin 895’cuttings. The soluble sugar content and the related enzyme activity of "sink" organ were higher than "source" organ. It was speculated that the exogenous Ca~(2+) could enhance the "sink’s" strength, and accelerate the assimilation transportation from "source".
     5. The stable isotope value of carbon and nitrogen enrichment capacity of Populus×euramericana‘Nanlin 895’cuttings were affected by exogenous Ca~(2+). With the Ca~(2+)concentration increasing, theδ13C,δ15N value of cuttings were presented gradually trends of increase in advance, and then decrease without exception. The distribution of plants’δ13C,δ15N value should be obeyed the principle that the "sink" organ was bigger than the "source" organ.
     6. It had a certain accumulative effect that the exogenous Ca~(2+) played a vital role in promoting the growth of Populus×euramericana‘Nanlin 895’cuttings. The positive effect of lower Ca~(2+) concentration gradually were enhanced, while the negative effect of higher Ca~(2+) concentration has gradually started to emerge as the extension of growth period.
引文
[1]艾天成,周治安,李方敏,等.小麦等作物叶绿素速测方法研究[J].甘肃农业科技,2001,(4):16-18.
    [2]曹恭,梁鸣早.钙-平衡栽培体系中植物必需的中量元素[J].土壤肥料,2003,2:48-49.
    [3]蔡德陵,毛兴华,韩贻兵.13C/12C比值在海洋生态系统营养关系研究中的应用[J].海洋与湖沼,1999,30(3):306-314.
    [4]蔡德陵,张淑芳,张经.天然存在的碳、氮稳定同位素在生态系统研究中的应用[J].质谱学报,2003,24(3):434-440.
    [5]陈良,温贤芳,郭智芬.稳定同位素15N在我国农业研究中的应用进展[J].同位素,1991,4(1):60-64.
    [6]陈焱丽,王清连,石明旺,等.大豆源库流的研究进展[J].安徽农业科学,2007,35(8):2251-2252.
    [7]陈双林,吴柏林,张德明.林地覆盖对红竹林温度和出笋时间分配的影响研究[J].林业科学究,2004,17(专刊):90-94.
    [8]崔杰华,祁彪,王颜红.植物样品中稳定碳同位素的EA2IRMS系统分析方法[J].质谱学报,2008,29(1):24-29.
    [9]蔡金华,糜林.无花果秋季同化物的贮藏及转运[J].山东农业大学学报,2003,34(3):366-368.
    [10]陈明,姚允寅,张希忠.不同形态氮素对15N同位素稀释法估测苜蓿固氮的影响[J].核农学通报,1997,18(1):30-40.
    [11]陈世苹,白永飞,韩兴国.稳定性碳同位素技术在生态学研究中的应用[J].植物生态学报, 2002,26(5):549-560.
    [12]丁彬,岳晓翔,彭建云等.钙对NaCl胁迫下百合花粉萌发、脯氨酸含量和SOD活性的影响[J].菏泽学院学报.2006,28 (5):98-102.
    [13]董彩霞,周健民,赵世杰等.外源钙对不同钙敏感型番茄幼苗生理特性的影响[J].应用生态报.2005,16(2):267-272.
    [14]段留生,何钟佩,韩碧文.小麦籽粒发育期间旗叶碳同化物的输出和分配特征[J].中国农业大学学报,2000,5(1):69-74.
    [15]丁明明,苏晓华,黄秦军.欧洲黑杨基因资源稳定碳同位素组成特征[J].林业科学研究,2006,19(3):272-276.
    [16]丁九敏,高洪斌.黄瓜霜霉病抗性与叶片中生理生化物质含量的关系的研究[J].辽宁农业科学,2005,1:11-13.
    [17]董志新,李绍长,张煜星,等.大豆源库间物质转化及同化物运输规律[J].新疆农业科学,2001,38(4):174-176.
    [18]樊成,刘运良.不同钙肥对苹果品质的影响及其作用机理[J].腐质酸,2005,31-34.
    [19]冯虎元,安黎哲,王勋陵.环境条件对植物温度碳同位素组成的影响[J].植物学通报,2000,17(4):312-318.
    [20]傅金民等.大产量形成期光合速率和库源调节效应[J].中国油料作物学报,1998,20(1):51-55.
    [21]范爽,高东升,赵海亮.设施栽培条件下桃树果实不同发育时期14C同化物的运转及分配性[J].核农学报,2006,20(3):241-244.
    [22]范双喜,伊东正.钙素对叶用莴苣营养吸收和生长发育的影响[J].园艺学报.2002,29(2):149-152.
    [23]范武刚,张治民,杜明叶等.早熟桃果缺钙症的发生与防治[J].西北园艺.2008.2:53-53.
    [24]洑香香,方升佐,田野,等.钙离子浓度对青檀生长和檀皮质量的影响. [J]应用生态报,2004,15(9):1502-1506.
    [25]范仲学,王璞,梁振兴.利用同位素示踪研究夏玉米灌浆期同化产物的运转[J].核农学报2001,15(1):32-37.
    [26]关军锋,马智宏,李红琴.Li+新霉素和Ca2+对苹果花粉萌发与花粉管生长的影响[J].武汉植物学研究,2000,18(1):64-66.
    [27]龚明,杨中汉,曹宗翼.钙对花粉萌发的启动效应和对花粉管生长的调节作用[J].北京大学学报(自然科学版),1995,31(2):238-249.
    [28]刚明慧,齐曼.尤努斯,覃伟铭,等.不同植物生长调节剂对库尔勒香梨坐果率、脱萼果率、果实形态及叶绿素含量的影响[J].新疆农业大学学报,2009,32(6):26-30.
    [29]郭卫东,李嘉瑞.猕猴桃不同时期14C同化物贮藏动态及再利用规律[J].西北农业学报,1999,8(3):86-88.
    [30]黄东杰,张树珍,范海阔,等.甘蔗的蔗糖代谢[J].植物生理通讯,2006,42(4):1-6.
    [31]黄化刚,李廷轩,张锡洲.外源钙离子对东南景天生长及锌积累的影响[J].应用生态学报,2008,19(4):831-837.
    [32]何龙飞,沈振国,刘友良.铝胁迫下钙对小麦液泡膜功能和膜脂组成的影响[J].南京农业大学学报,2000,23(1):10-13.
    [33]胡金朝,施国新,王学,等.Cd对槐叶苹的生理影响及外源La、Ca的缓解效应比较[J].广西植物.2005,25(2):156-160.
    [34]胡梦芸,张正斌,徐萍.植物光合产物转运蛋白及其生物学功能[J].植物生理学通报,2008,44(1):1-6.
    [35]洪伟,黄锦湖,李键等.不同桉树品种稳定碳同位素研究[J].福建林学院学报,2008,28(3):193-197.
    [36]韩晓日,姜琳琳,王帅,等.不同施肥处理对春玉米穗位叶光合指标的影响[J].沈阳农业大学学报,2009,40(4):444-448.
    [37]贾忠峰,高桂生,李忠贤.芹菜缺钙性生育障碍及防治[J].农业科技与信息,2008,(05):18-18.
    [38]金爱红,储立民,徐冬青等.钙对葱兰花粉萌发和花粉管生长的影响[J].湖北农业科学,2005,03:91-93.
    [39]纪凤奎,高爱红,周元明.产量与源库的关系及其在作物高产中的意义[J].垦殖与稻作,2004.02:9-10.
    [40]江解增,邱届娟,曹碚生等.茭白膨大前后源库关系的研究[J].扬州大学学报(自然科学版),2001,4(4):39-42.
    [41]景岚,王丽芳,康俊.不同抗性的向日葵品种接种锈菌后叶片中可溶性蛋白、可溶性总糖及叶绿素含量的变化[J].临沂师范学院学报,2008,30(6):76-80.
    [42]孔云,王绍辉,马承伟等.轻度遮光对温室油桃结果枝光合碳同化物积累和分配的影响[J].农业工程学报,2007,23 (3):169-173.
    [43]李德军,莫江明,方运霆,等.氮沉降对森林植物的影响[J].生态学报,2003,23(9):1891-1900.
    [44]李功藩,吴亚君,刘冬,等.光系统Ⅱ颗粒的多肽组成分析和重组后的放氧活性[J].植物生理学报.1987,13(4):351-358.
    [45]李天来,李淼,李益清,等.CaCl2和水杨酸对昼间亚高温胁迫下番茄叶片光合作用的影响[J].西北农业学报,2009,18(4):284-289.
    [46]刘彩霞,蒋学皎,陈进红.作物蔗糖磷酸合成酶(SPS)活性调控的研究进展[J].科技通报,2008,24(3):355-360.
    [47]刘凤霞.植物生理学中“源库流”的教学[J].河南科学,1996,14(增刊):141-142.
    [48]刘慧英,朱祝军.转化酶在高等植物蔗糖代谢中的作用研究进展[J].植物学通报,2002,19(6):666-674.
    [49]刘玲,周桃华.外源钙离子(Ca2+)对万寿菊幼苗抗冷性的影响[J].安徽农学通报[J],2008,14(18):113-114.
    [50]刘全吉,孙学成,胡承孝,等.砷对小麦生长和光合作用特性的影响[J].生态学报,2009,29(2):854-859.
    [51]刘晓冰,崔守福.源库改变对小麦籽粒蛋白质、淀粉含量及产量的影响[J].种子,1995,5:5-7.
    [52]卢合全,沈法富,刘凌霄等.植物蔗糖合成酶功能与分子生物学研究进展[J].农业生物技术科学,2005,21(7):34-37.
    [53]刘海燕,李吉跃,赵燕等.沙柳稳定碳同位素值的特点及其水分利用效率[J].干旱区研究,2008,25(4):514-518.
    [54]刘微,吕豪豪,陈英旭.稳定碳同位素技术在土壤-植物系统碳循环中的应用[J].应用生态学报,2008,19(3):674-680.
    [55]李建国,黄辉白,黄旭明.妃子笑荔枝早花大果和晚花小果与营养竞争的关系[J].果树学报,2003,20(2):195-198.
    [56]吕金印,山仑,高俊凤.水分亏缺对小麦碳同化物的动员与分配[J].核农学报,2002,16(4):228-231.
    [57]吕金印,刘军,高俊凤.春小麦花前14C同化物分配与累积研究[J].核农学报,1999,13(6):357-361.
    [58]刘凌霄,沈法富,卢合全,等.蔗糖代谢中蔗糖磷酸合成酶(SPS)的研究进展[J].分子植物育种,2005,3(2):275-281.
    [59]李湘麟,熊月明,陆怪闽,等.柑桔钙素营养研究综述[J].福建果树,2001,115(l):13-20.
    [60]李明财,黎贞发,易现峰.青藏高原东部高寒草甸植物δ13C年变化及其环境分析[J].生态环境,2007,16(4):1205-1210.
    [61]李明财,罗天祥,刘新圣等.高山林线急尖长苞冷杉不同器官的稳定碳同位素组成分布特征[J].应用生态学报,2007,18(12):2654-2660.
    [62]吕英民,张大鹏.果实发育过程中糖的积累[J].植物生理学通讯,2000,36(3):258-265.
    [63]刘洋,龙应霞,文治瑞.稳定碳同位素技术在植物水分利用效率研究中的应用[J].黔南民族师范学院学报,2008, 03:59-61.
    [64]刘祖祺,张石城.植物抗性生理学[M].北京:农业出版社,1994,371-372.
    [65]梁颖,王三根.Ca2+对冷害水稻幼苗某些生理特性的影响[J].西南师范大学学报(自然科学版).1997,22(4):411-415.
    [66]李延菊,李宪利.设施油桃膨大期摘心和疏果对根外追15N的运转与分配的影响[J].中国生态农业学报,2008,16(1):117-120.
    [67]刘颖慧,贾海坤,高琼.植物同化物分配及其模型研究综述[J].生态学报,2006,26(6):1982-1992.
    [68]李玉平,江小清,刘苑秋.碳、氮同位素示踪法在农林业中的应用[J].江西科学,2007,25(5):582-587.
    [69]郎志红,陶玲.钙对盐胁迫下芨芨草萌发与生长的缓解效应[J].环境科学与管理.2008,33(4):130-133.
    [70]毛俊娟,倪婷,王胜华,等.干旱胁迫下外源钙对麻疯树相关生理指标的影响[J].四川大学学报(自然科学版),2008,45(3):669-673.
    [71]孟宪局,张平,刘铜.用15N示踪法研究人参吸氮及其对14C同化物分配的影响[J].核农学报,1999,13(1):34-38.
    [72]马淑英等.钙对拟南芥耐盐性的调节[J].作物学报.2006,32(11):1706-1711.
    [73]宁代峰.杨树光合同化物卸载的细胞学路径及其生理生化机制[M].硕士学位论文,2008.
    [74]潘一山,蔡晓东,曹芳.龙柚果肉糖积累与蔗糖代谢相关酶活性的研究[J].亚热带植物科学,2006,35(3):16-17.
    [75]潘瑞炽,王小菁,李娘辉.植物生理学[M].第四版,北京:高等教育出版社,2000.
    [76]潘瑞炽,王小菁,李娘辉.植物生理学[M].第四版,北京:高等教育出版社,2004.
    [77]钱树林.唐菖蒲籽球发育特点及其碳水化合物代谢变化[J].中国农业大学学报,2007,12(2):34-39.
    [78]钱骅,刘友良.盐胁迫下钙对大麦根系质膜和液泡膜功能的保护效应[J].植物生理学通讯,1995,31(2):102-104.
    [79]苏波,韩兴国,黄建辉.15N自然丰度法在生态系统氮素循环研究中的应用[J].生态学报,1999,19(3):408-416.
    [80]苏艳,原牡丹,侯智霞,等.蔗糖代谢相关酶在果实中的作用[J].北方园艺,2008,(9):50-54.
    [81]尚忠林,李建华,李冰.钙通道抑制剂对川百合花粉萌发的影响[J].河北师范大学学报(自然科学版),2004,28(5):510-513.[82]隋方功,葛体达,刘鹏起,等.干旱对夏玉米碳素同化、运转与分配的影响研究[J].中国生态农业报,2006,14(3):234-237.
    [83]隋方功,王运华.植物碳素营养研究中碳同位素示踪技术的应用及进展[J].莱阳农学院学报,2001,18(2):107-111.
    [84]史树德,贺学勤,张子义,等.甜菜蔗糖代谢两种相关酶的活性变化及其相互关系[J].华北农报,2008,23(3):120-124.
    [85]孙宪芝,郭先锋,郑成淑,等.高温胁迫下外源钙对菊花叶片光合机构与活性氧清除酶系统的影响冰[J].应用生态学报, 2008,19(9):1983-1988.
    [86]苏艳,原牡丹,侯智霞,等.蔗糖代谢相关酶在果实中的作用[J].北方园艺,2008,09:50-54.
    [87]陶文菁.烟草花粉萌发和花粉管生长过程中钙调素的分布及功能研究[M].博士论文,2004.
    [88]魏长宾,武红霞,马蔚红,等.芒果成熟阶段蔗糖代谢及其相关酶类研究[J].西南农业学报,2008,21(4):972-974.
    [89]王宝增,张新军.外源钙对玉米幼苗耐盐性的影响[J].黑龙江农业科学,2009,03:50-51.
    [90]王光,钦佩,宰学明.叶面喷Ca2+增加海滨锦葵幼苗对高温的适应能力[J].生态与农村环境学报.2006,22(3):41-44.
    [91]王海华,谭新中,康健,等.钙对水稻幼苗镍毒害的缓解效应[J].农业环境科学学报,2003,22(3):357-359.
    [92]王利芬,夏仁学,周开兵.纽荷尔脐橙果肉糖分积累和蔗糖代谢相关酶活性的变化[J].果树学报,2004,21(3):220-223.
    [93]王晶英,张兴梅,李国兰.钙对大豆生长及产量的影响[J].中国油料作物学报,2004,26(1):60-62.
    [94]韦剑锋,韦冬萍,何燕文等.喷施钙硼对龙眼叶片和果实矿质营养状况的影响[J].西南农业学报,2007,20(1):77-80.
    [95]王巧兰,吴礼树,赵竹青.15N示踪技术在植物N素营养研究中的应用及进展[J].华中农业大学学报,2007,26(1):127-132.
    [96]吴文新,陈家驹,周恩生,等.钙硼对花生生长产量和品质的影响[J].亚热带植物科学,2001,30(2):20-23.
    [97]王晓玲,石雷,孙吉雄,等.遮荫对山麦冬生长特性和生物量分配的影响[J].植物研究,2006,26(2).
    [98]王志强,王春丽,林同保.外源钙离子对小麦幼苗氮素代谢的影响.生态学报,2008,28(8):3662-3667.
    [99]魏书銮,于继洲,宣有林,等.核桃叶片的叶绿素含量与光合速率关系的研究[J].北京农业科学,1994,12(5):3l-33.
    [100]辛建华,李天来,陈红波.外源钙处理对马铃薯块茎重量和数量的影响[J].西北农业学报, 2008,17(5):248-251.
    [101]薛慧勤,甘信民等.旱条件下花生水分利用效率与叶片碳同位素辨别力的相关性研究[J].中国油料作物学报,1999,21(1):27-30.
    [102]薛延丰,刘兆普.钙离子对盐胁迫下菊芋幼苗的生长、生理反应和光合能力的影响理论[J].农业工程学报.2006,22(9):44-47.
    [103]薛延丰,刘兆普.外源钙离子缓解海水胁迫下菊芋光合能力下降的研究[J].草业学报,2007,16(6):74-80.
    [104]许大全.光合作用气孔限制分析中的一些问题[J].植物生理学通讯,1997,33(4):241-244. [105 ]盛仙永,胡正海.Ca2+、pH在花粉及萌发花粉管生长中的作用研究进展.西北植物报,2005,25(1):0194-0199.
    [106]熊福生,高煜珠,詹勇昌,等.植物叶片蔗糖、淀粉积累与其降解酶活性关系研究[J].作物报,1994,20(1):52-58.
    [107]徐秋曼,陈宏,程景胜.外源Ca2+对水稻幼苗生长的影响[J].天津师大学报(自然科学版),1999,19(4):49-58.
    [108]夏宜平,郑慧俊,黄春辉,等.东方百合鳞茎更新发育的碳同化物积累与分配[J].核农学报,2006,20(5):417-422.
    [109]夏宜平,杨玉爱,杨肖娥,等.郁金香更新鳞茎发育的碳同化物积累与内源激素变化研究[J].园艺学报,2005,32(2): 278-283.
    [110]于佰双.15N示踪技术在大豆研究领域的应用概况[J].黑龙江农业科学,1995,06:35-37.
    [111]袁清昌.钙提高植物抗旱能力的研究进展[J].山东农业大学学报,1999,30(3):302-306.
    [112]于凤义,张玉文,张萍.春小麦灌浆期功能叶14C同化物源/库调控[J].华北农学报,1997,12(3):30-35.
    [113]雍立华,李树华,许兴,等.小麦碳同位素分辨率与抗旱生理、农艺性状的相关研究[J].宁夏农林科技,2007,(2):9-12.
    [114]杨俊峰,龚月桦,王俊儒,等.旱地覆膜对冬小麦花后14C同化物转运分配的影响[J].核农报,2007,21(1):70-74.
    [115]尹伟伦,万雪琴,夏新莉.杨树稳定碳同位素分辨率与水分利用效率和生长的关系[J].林业科学,2007,43(8):15-22.
    [116]尹增芳,何祯祥,陈梦阳,等.外源钙对NaCl胁迫下海滨锦葵种子萌发和幼苗生长的缓解效应[J].西北植物学报.2006, 26(3):0462-0466.
    [117]姚艳丽,杨国正,宋峥,等.壮苗素对油菜幼苗可溶性糖和蛋白质含量的影响[J].湖北农业科学,2008,47(3):283-286.
    [118]苑智华,何秀丽,徐哲,等.唐菖蒲球茎形成期蔗糖和淀粉代谢及其相关酶活性[J].林业科学,2008,44(8):47-51.
    [119]赵炳梓,张佳宝.旱稻水分利用率与碳同位素识别值之间的关系[J].土壤学报,2004,41(5).
    [120]张丛志,张佳宝.稳定同位素在作物水分关系研究中的应用[J],土壤学报,2008,40(2):167-173.
    [121]赵凤霞,姜远茂,彭福田,等.甜樱桃对15N尿素的吸收、分配和利用特性[J].应用生态学报,2008,19(3):686-690.
    [122]郑国琦,罗霄,郑紫燕等.宁夏枸杞果实糖积累和蔗糖代谢相关酶活性的关系[J].西北植物学报,2008,28(6):1172-1178.
    [123]张帆,罗承德,张健.外源钙、磷、氮对铝胁迫下杉木幼苗生长影响的调控研究[J].应用生态学报,2005,16(2):213-217.
    [124]赵凤君,高荣孚,沈应柏,等.水分胁迫下美洲黑杨不同无性系间叶片δ13C和水分利用效率的研究.林业科学,2005,41(1):36-41.
    [125]张翠翠.杏树叶片叶绿素含量和比叶重变化的研究[J].安徽农业科学,2007,35(16):4762-4763.
    [126]张凌云,张大鹏.光合同化物韧皮部卸载途径和机制.植物生理学通讯,2003.39(4):399-403.
    [127]张明方,李志凌,陈昆松,等.网纹甜瓜发育果实糖分积累与蔗糖代谢参与酶的关系.植物生理与分子生物学报,2003,29(5):455-462.
    [128]张明方,李志凌.高等植物中与蔗糖代谢相关的酶[J].植物生理学通讯,2002,38(3):289-295.
    [129]张明生,谢波,谈锋.甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究[J].中国农业科学,2003,36(1):13-l6.
    [130]张建霞,李新国,杨波,等.Ca2+对冷胁迫柑橘离体叶片的相关生理生化指标的影响[J].武汉植物学研究,2005,23(5):470-474.
    [131]张建霞,李新国,孙中海.外源钙对柑橘抗热性的相关生理生化指标的影响[J].华中农业大学学报,2005,24(4):397-400.
    [132]周平,叶冰莹,陈由强,等.蔗糖磷酸合成酶研究的新进展.生物技术通讯[J],2006,06:1001-1003.
    [133]张蓓.在小麦受叶锈菌侵染过程中特异性钙信号形成机制的探究[M].博士论文,2008.
    [134]张起刚,何昌永,王化国,等.应用15N示踪技术研究冬灌对小麦生长发育及吸收N素的影响[J].核农学通报,1993,14(4):172-176.
    [135]张志良.植物生理学实验指导[M].北京:高等教育出版社,1990,154-158.
    [136]张宗申,利荣千,王建波.Ca2+预处理对热胁迫下辣椒叶肉细胞中Ca2+-ATPase活性的影响[J].植物生理学报,2001,27(6):451-454.
    [137]宰学明,吴国荣,钦佩等.Ca2+对花生幼苗耐热性的影响[J].南京林业大学学报(自然科学版),2006,30(3):47-50.
    [138]张晓燕,商振清等.抗病毒剂VA诱导烟草对TMV的抗性与Ca2+的关系[J].河北农业大学学报.2004,27(2):17-20.
    [139]张秀梅,杜丽清,孙光明,等.菠萝果实发育过程中糖积累与其代谢酶的关系[J].热带作物学报,2008,29(1):10-13.
    [140]章泳,俞炳呆,王薛修.氯化钙对批把贮藏期的效应及其作用机理[J].南京农业大学学报,1995,18(1):104-105.
    [141]朱林,许兴,李树华等.春小麦碳同位素分辨率的替代指标研究[J].西北植物报,2006,26(7):1436-1442.
    [142]朱晓军,杨劲松,梁永超,等.盐胁迫下钙对水稻幼苗光合作用及相关生理特性的影响[J].中国农业科学,2004,37(10): 147-150
    [143]赵希岳,龚荐,罗时石,等.棉花叶片光合产物输配的动力学研究[J].核农学报,2002,16(1):20-25.
    [144]周裕荣,陈明莉.14C同化物在黄花菜植株内的运输及分配研究[J].西南农业大学学报,1996,18(4):316-319.
    [145]左秀丽,刘文进,夏春婷.黄瓜缺钙症状与施肥技术[J].河北农业科技,2006,12-12.
    [146] Abdul K,Hiroshi F,Tetsushi H. Phtotosynthetic performance of Vigna radiate L.leaves developed at different temperature and irradiance leaves[J].Plant Science,2003,164:451-458.
    [147] Araus J L, Villegas D,Aparicio N, et al.2003.Environmental factors determining carbon isotope discrimination and yield in durum wheat under Mediterranean conditions[J].CropScience,43(1):170-180.
    [148] Arora R,Palta J P.In vivo perturbation of Membrance-Associated Calcium by Freeze-Thaw stress in Onion Bulb cells simulation of this perturbation in extracellular kci and alleviation by calcium[J].plant physiol,1988,87:622-628.
    [149] Badeck F W,Tcherkez G,Nogues S,et a1. Post-photosynthetic fractionation of stable carbon isotopes between plant organs:A widespread phenomenon[J].Rapid Communications in Mass Spectrometry,2005,19:1381-1391.
    [150] Bangerth F.Calcium related physiological disorders of plants[J].Annu.Rev.phytopathol,1979,17:97-122.
    [151] Beruter J,Studer Feusi M E.Comparision of sorbitol transport in excised tissue discs and cortex tissue of intact apple fruit.Plant [J]. Physiol,1995,146:95-102.
    [152] Brewbaker J L,Majumder S M.Cultural studies of the pollen population effect and the self-incom- patibility inhibition[J].Amer J Bot,1961,48:457-460.
    [153] Brewbaker J L,Kwack B H.The essential role of calcium ion in pollen tube germination and pollen tube growth[J]. Am.J.Bot.,1963,50:859-865.
    [154] Chiou T J,Bush D R.Sucrose is a signal molecule in assimilate partitioning[J].Proc Natl Acad Sci USA,1998,95: 4784-4788.
    [155] Chengappa S,Guilleroux M,Phillips W.Transgenic tomato plant swith decreased sucrose synthase are unaltered in starch and suga raccumulation in the fruit [J].Plant Molbiol,1999,40:213-221.
    [156] Cramer G R, Ep stein E,LauchliA. Na+、Ca2+interactions in barley seedlings:relationship to transport and growth[J]. Plant,Cell and Environ.1989,12:551-558.
    [157] Cramer G R,Lauchli A,Epstein E.Effects of NaCl and CoC/Zon ion activities in Complex nutrient Solutions and root growth of Cotton[J].Plant phsiol,1986,81:792-797.
    [158] Condon A G, Richards R A,Rebetzke G J,et al.Improving intrinsic water-use efficiency and crop yield[J].Crop Sci,2002,42:122-131.
    [159] D.Aoust M A,Yelle S,Nguyen-Quoc B.Antisense inhibition of tomato fruit sucrose in hetero trophic cell suspension cultures of Chenopodiumrubrum L[J].Planta,1999,182:223-231.
    [160] Dai G X, Peng K Q , Pi C H.The effects of calcium on salt tolerance in plant[J].Chinese Agricultural Science Bulletin, 2003,19(3):97-101.
    [161] Dawson T E,Mambelli S,Plamboeck A H et al,. Stable isotopes in plant ecology[J].Annual Review of Ecology and Systematic,2002(33):507-599.
    [162] Deines P.The isotopic composition of reduced organic carbon[A].In:Fritz,P.(eds).
    [163] Doehlert D.C,and Huber S.C, Regulation of spinach leaf sucrose phosphate synthase by glucose-6-phosphate,inorganic phosphate,and pH[J].Plant Physiol,1983,73(4):989-994.
    [164] Du L Q,B.W.Poovaiah.Ca2+/calmodulin is critical for brassinosteroid biosynthesis and plant growth[J].Nature,2005,437: 741-745.
    [165] Equiza M A,Mirac E J,Tognettij A.Differen tialinhibition of shoot vs.Root Growth at low temperature and its relationship with carbohydrate accumulation in different wheat Cultivars[J].Annu Bot,1997,80:657-663.
    [166] Erik T,Nilsen AND M ,Rasoul S,et al.Carbon isotopic compositon of legumes with Photosynthetic stems from Mediterranean and Desert habitats.American Journal of Botany[J],1997,84(12):1707-1713.
    [167] Farquhar G D,Sharkeyu T D.Stomatal conductance and photosynthesis[J].Ann.Rev.Plant Physiol.,1982,33:317-345.
    [168] Foyer C H,Noctor G.Photosynthetic nitrogen assimilation: inter-pathway control and signaling.In:Foyer C H,Noctor G,eds.Photosynthetic-NitrogenAssimilation and Associated Carbon and Respiratory Metabolism[J].Dordrecht: Kluwer Academic Publishers, 2002,1-22.
    [169] Frankling-Tong V E.Signaling and the modulation of pollen tubegrowth[J].PlantCell,1999(11):727-738.
    [170] Francey R J,Giord R M,Sharkey T D,et al.Physio-logical influences on carbon isotope-discrimination in huon pine(Lagarostrobus franklinii) [J].Oecologia,1985,66:469-481.
    [171] Furquhar G D,Ehleringer J R,Hubick K T.Carbon isotope discrimination and photosynt hesis[J].Ann Rev Plant Physical Plant Mal Bial,1989,40:503-537.
    [172] Gao H B,Chen G L.The Effect of Calmodulin Antagonist and Calcium on Chilling Resistance of eggplant Seedling[J]. Acta Horticulturae Sinica.2002,29(3):243-246.
    [173] Giselle Martínez-N.Jorge Tognetti.Vinay Nagaraj.Andres Wiemken.Horacio Pontis.Calcium is essential for fructan synthesis induction mediated by sucrose in wheat Planta[J],2006,225:183-191.
    [174] Gong M,Cao Z X.Regulation of Calcium and Calmodulin on pollen Germination and Growth of Pollen Tube[J].Plant Physiology Communications,1995,31(5):321-328.
    [175] Gong Y H,Gao J F. Transport and Partitioning of Photoassimilate in Higher Plant[J].Acta Bot.Boreal, Occident.Sin. 1999,19(3):564-570.
    [176] Gong Y H,Zhang J,GAO J F,Lu J Y,et al.Slow Export of Photoassimilate from Stay Green Leaves During Late Grain-Filling Stagein Hybrid Winter Wheat(Triticum aestivum L.)[J].J Agron Crop Sci,2005,191:292-299.
    [177] Guerrieri M R,Siegwolf R T W,Saurer M,et al.Impact of different nitrogen emission sources on tree physiology as assessed by a triple stable isotope approach[J].Atmospheric Environment,2009,43:410-418.
    [178] Guo KE,M arinus J A,W erger,D ifferent responses to shade and deciduous oak seedlings and the effect of acorn size[J].Acta Oecologica,1999,20(6):579-586.
    [179] Handbook of Environment Isotope Geochemistry[C].New York:Elsevier,1980, 329-406.
    [180] Hayata Y,Li X X, Osajima Y.Sucrose accumulation and related metabolizing enzyme activities in seeded and induced parthenocarpic muskmelons[J].J Am Soc Hortic Sci,2006,126:676-680.
    [181] Harbron S.,Foyer C.,and Walker D. The purification and properties of sucrose-Phosphate synthetase from spinach leaves:the involvement of this enzyme and fructose biophosphatase in the regulation of sucrose biosyn-thesis[J]. Arch.Biochem.Biophys,1981,212:237-246.
    [182] He L F, Shen Z,Liu Y L. Effects of calcium on the function and lipid composition of tonop last from two wheat (Triticum aestivum L.) cultivar roots under alum inum stress[J].Journal of Nanjing Agricultural University,2000, 23(1):10-13.
    [183] Hepler P K.Tip growth in pollen tubes:Calcium leads the way [J].Trends Plant Sci,1997,(2):79-88.
    [184] Hobbie E A,Macko S A,Williams M.Correlations between foliarδ15N and nitrogen concentrations may indicate plant mycorrhizal interactions.Oecologia, 2000,122:273-283
    [185] Hogberg P and Alexander I J. Roles of root symbioses in African woodland and forest: evidence from 15N abundance and foliar analysis[J].Journal of Ecology,1995,83:217-224.
    [186] Hogberg P.Nitrogen impacts on forest carbon[J].Nature,2007,447:781-782.
    [187] McArthur J V and Moorhead K K. Characterization of riparian species and stream detritus using multiple stable isotopes [J].Oecologia,1996,107:232-238.
    [188] Huber S.C.Role of sucrose-phosphate synthase in partitioning of carbon in leaves[J].Plant Physiol.1983,71:818-821.
    [189] Impa S M,Nadaradjan S,Boominathan P,et al. Carbon isotope discrimination accurately reflects variability in WUE measured at a whole plant level in rice[J].Crop Science,2005,45(6):2517-2522.
    [190] Inanaga and Yoshihara.Translocation and distribution of assimilated carbon in peanut plant[J].Soil science and plant nutrition,1997,43(2):267-274.
    [191] Jiang G H, Xie M, Fang L.et al. Effects of boron, calcium, pesticide and germicide on pollen germina- tion and tube growth of strawberry[J].Journal of Fruit Science,2007,24(2):234-236.
    [192] Jiang Y B,Cui G W,Li H.Effect of coating Medica go s ativa seeds in calcium on drought resistance and interrelatedphysiological indexes under drought conditions[J].Acta pratacul tyrae subuca,2005,14(5).
    [193] Jiang X Y,Song J,Fan H. et al. Regulations of Exogenous Calcium and Spermidine on Ion Balance and Polyamine Levels in Maize Seedlings Under NaCl Stress[J].Acta Phyto-physiologica Sinica.2000,26(6):539-544.
    [194]Jiang L G,Dai T B,Jian G D,et al.Characterizing physiological N2 use efficiency as influenced by nitrogen managementin three rice cultivars[J].Field Crops Research,2004,88:239-250.
    [195] Jorg-Peter,Schnitzler. Stable Isotopes,Plant VOCs and Secondary Organic Aerosols in Biosphere-Atmosphere Carbon Exchange[J].ISONET-VOCBAS Short Course,Kuopio,Finland,2006,28-30.
    [196] Knight H, Trewaras A J, Knight M R.Calcium signaling in Arabidopsis thliana responding to drought and salinity [J]. Plant J,1997,12(5):1067-1072.
    [197] Liang W J,Wang M L,Ai X Z.The role of calcium in regulating photosynthesis and related physiological indexes of cucumber seedlings under low light intensity and suboptimal temperature stress[J].Scientia Horticulturae,2009(123):34-38.
    [198] Li Y.G,Yu Z.W,Jiang D,and Yu S.L. Studies on the dynamic changes of the synthesis of sucrose in the flag leaf and starch in the grain and related enzymes of high-yielding wheat[J].Acta Agronomica Sinica, 2001,27(5):658-664.
    [199] Li Q Y, Ge H B, Hu S M.et al. Effects of Exogenous Calcium on Chlorophyll Fluor escence Par ameter of Strawberry under Salt Stress[J].Journal of Shenyang Agricultural University,2006,37(3):482-484.
    [200] Lrnvell C A,Tomlinson P T, Koch K E et al.Sucrose-metabolizing enzymes in transport and adjacent sink structure in developing citrus fruit[J].Plant Physiol,1989,90:1394-1402.
    [201] Mediavilla S,Santuago H,Escudero A.Stomatal and mesophyll limitations to photosynthesis in one evergreen and one deciduous mediterranean oak species[J].Photosynthetica,2002,40:553-559.
    [202] Michopoulos P,Baloutsos G,Economou A,et al.Effects of nitrogen deposition on nitrogen cycling in an Aleppo-pine stand in Athens,Greece[J].Science of the Total Environment,2004,323:211-218.
    [203] Mohapatra PK,Masamoto Y,Morita S,et al.Partitioning of 13C-labelled photosynthate varies with growth stage and panicle size in high-yielding rice[J].Functional Plant Bioloy,2004,31:131-139.
    [204] Mukherjee,S.P,M.A.Choudhwri. Effect of water stress on some oxidative enzymes and senescence invigna seed-lings[J]. Physiol Plant,1981,52:37-42.
    [205] Nathalie Galtier,Christine H.Foyer,Joan Huber.Effects of evevated sucrose phoshpate synthase acticitivies on photosynthesis,assimilate partition in and growth in tomato[J].Plant Physiol,1993,101:535-543.
    [206] Nissenbaum A, ShallingerKM.The distribution of the stable carbon isotope(13C/12C) in fractions of soil or-ganicmatter[J]. Geoderma,1974,11:137-145.
    [207] Oparka K J.What is phloem unloading[J].Plant Physiol.1990,94:393-396.
    [208] Patrick J W.Phloem unloading:sieve element unloading and post sieve element transport[J].Annu Rev Plant Physiol Plant Mol Biol,1997,48:191-222.
    [209] Qi H Y,Li T L,Liu H T,Zhang J.St udies on carbohydrate content and sucrose metabolizing enzymes activities in different parts of tomato[J].Acta Horticult urae Sinica,2005,32(2):239-243.
    [210] Radajewski S,Ineson P,Parekh N,et al.Stable-iso-tope probing as a tool in microbial ecology[J].Nature,2000,403: 646-649.
    [211] Rebetzke G J,CondonA G,RichardsR A,et al.Selection for reduced carbon isotope discrimination increases aerial biomass and grain yield of rainfed bread wheat[J].Crop Science,2002,42(3):739-745.
    [212] Ren L H,Weng B Q, Fang J M. Progress about the relationship between calcium fertilizers and drought-resistance of plants[J].Subtropical Agriculture Research.2005.8,Vol.1,No.3.
    [213] Richards R A,Condon A G.Challenges ahead in using carbon isotope discrimination in plant breeding program Ehleringer J R,et al.Stable isotopes and plant carbon water relations[M].Academic Press,New York,2002,451-462.
    [214] Royo C, Martos V,Ramdani A,et al.Changes in Yield and Carbon Isotope Discrimination of Italian and Spanish Durum Wheat during the 20th Century.American Society of Agronomy,2008,100:352-360.
    [215] Ruan Y L,Churey P S.A fiberles seed mutation in cotton is associated with lack of fiber initiation in ovule epidermis andalterations in sucrose sybthase expressio and carbon partitioning in developing seeds[J]. Plant Physiol.1998,118(2): 399-406.
    [216] Sanders D,Brownlee C,Harper J F.Communicationwith calcium[J].Plant Cell,1999(11):691-706.
    [217] Sandra L.Jackson,I.Brent Heath.Effects of exogenous calcium ions on tip growth,Intracell-ular Ca2+ Concentration,and actin arrays in hyphae of the fungus Saprolegnia ferax[J].Experimental Mycology,1989,13(1):1-12.
    [218] Schuhz H R,Mattews M A.Vegatative growth distribution during waterdeficits in Vitis viniferu L.[J1.Aust J Plant Physiol,1988,15:641-656.
    [219] Seekens S,Rook F,.ugar sensing and sugar-mediated signal transoluction in plant[J].Plant Physiol,1997,115:7-13.
    [220] Schmalstig JG, Hitz WZ. Contributions of sucrose synthase and invertase to the metabolism of sucrose in developing leaves[J].Plant Physiol,1987,85:407-412.
    [221] Sheng X Y,Hu Z H.Roles of Ca2+and pH in Pollen Germination and Pollen Tube Growth[J].Acta Bot.Boreal.Occident. Sin,2005,25(1):0194-0199.
    [222] Shivanna KR,Johri BM.The Angiosperm Pollen[J].New Delhi:Willey Eastern,1985,118-122.
    [223] Sun D Y,Tang W Q,Ma L G.Extracellular calmodulin:A polypeptide signal in plants[J].Science in China,Ser.C,2001,(05):449-460.
    [224] Xue Y F,Liu Z P et.Abating Effect of Calcium Ion on Seawater- stressed Jerusalem Artichoke (Helian thus tuberosus L. ) [J]. Acta Bot.Boreal.-Occident.Sin.2006, 26(6): 1267-1271.
    [225] Sheng L, Jrg K,Manuel R C,et al.Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants [J]. The Plant Cell,2002,5(14):389.
    [226] Skin K S,Chakrabarty D,Paek K Y.Sprouting rate,change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vitro[J].Scientia Hort,2002,96:195-204.
    [227] Very A A,Davis J M.Hyperpolarization-activated calcium channels at the tip of Arabidopsis root hairs [J].Proc Natl Acad Sci USA,2000,97:9801-9806.
    [228] Vizzotto G,Pinton R,Varanini Z,et al.Sucrose accumulation in developing peach fruit[J].Physiol Plant,1996,96:225-230.
    [229] Walley F L,Lafond G P,Matus A,et al.Water2Use efficiency and carbon isotopic composition in reduced tillage systems [J].Soil Sci.Soc.Am.J.,1999,63:356-361.
    [230] Wang Q L, Wu L S,Zhao Z Q. Advance and Application of 15N Tracer Method on Research of Plant Nitrogen Nutrition [J].Journal of Huazhong Agricultural University,2007,26(1):127-132.
    [231] Wang S P,Li J,et al,.Effects of NaCl stress on growth and photosynthetic characteristics of cucunber(cucumber sativus L.) seedlings[J].ActaBot.Boreal.Occident.sin.2006,26(3):455-461
    [232] Wright G C,Hubic K T,Farquhar G D,et al.Genetic and environmental variation intran- spiration efficiency and its correlation with carbon isotope discrim- ination and specific leaf area in peanut.In:Eh leringer J et al.(ed).Stable isotop- es and plant carbon-water relations[J].San D iego C A:A cadem ic P ress,1993,247-267.
    [233] Wu X Z,Yi Y.Effect of Ca2+ on the growth of Dendrobium nolie Lindl seedlings under drought stress[J].Journal of Guizhou NormalUniversity(NaturalSciences)2006,24(2).
    [234] Xu F A,Zhao B Z,Tang W L.Changes in some environmental factors and their ecological significance in north Henan Province,China[J].Acta Pedologica Sinica,200340(1):29-36.
    [235] Xu Z L,Yang Y H. Effect of Organic Manure Applied during Preceding Crops on Nitrogen Fertilizer Availability of Flue-cured Tobacco Using the Isotope 15N Trace Technique[J]. Chinese Agricultural Science Bulletin,2007,23(9).
    [236] Yu H W, L I Y. Research p rogress on salt tolerance of p lant[J]. Journal of Beihua University(Natural Sciences Edition). 2004,5(3):257-263.
    [237] Zavala J A,Ravetta D A.Allocation of photoassimilates to biomass,resin and carbohydrates in Grindelia chiloensis as affected by light intensity[J].FieldCrops Research,2001,69:143-149.
    [238] Zheng G Q,Xu X,et al,.The effect of saltstress on the stomatal and non-stomatal limitation of photosynthsis of Lycium barbarum[J].Acta Bot.Boreal.Occident.sin.2002,22(6):1355-1359.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700