摘要
本研究以864只河西绒山羊、223只柴达木绒山羊和633只陇东绒山羊(共计1720只)为研究对象,采用PCR-SSCP和克隆测序技术分析3个山羊群体DQA1基因第2外显子的多态性和遗传特征,并进行河西绒山羊流产关联性分析。主要研究结果如下:(1)在3个山羊群体中共发现9个DQA1等位基因,其中等位基因GoLA-DQA1*B、GoLA-DQA1*E和GoLA-DQA1*H为本研究新发现。9个等位基因均存在于河西绒山羊和陇东绒山羊中,但柴达木绒山羊中未检测到等位基因GoLA-DQA1*F、GoLA-DQA1*H。(2)9个等位基因中发现42个核苷酸变异位点和23个氨基酸多态位点。河西绒山羊GG、CC、DD为优势基因型,G为优势等位基因;柴达木绒山羊DG、DE和D为优势基因型和优势等位基因;陇东绒山羊DD和D为优势基因型和优势等位基因。遗传结构分析表明3个山羊群体具有丰富的遗传多样性,且都处于Hardy-Weinberg不平衡状态。(3)经SPSS分析表明,河西绒山羊不同表型间的等位基因差异显著(P<0.05),流产组中等位基因GoLA-DQA1*A的频率高于正常组(P<0.05),等位基因GoLA-DQA1*H的频率低于正常组(P<0.01)。其余各等位基因频率的分布无差异(P>0.05)。等位基因GoLA-DQA1*A可能与流产的易感性相关,GoLA-DQA1*H可能与流产抗性有关。
With the view of studying polymorphism of DQA1gene exon2in864HexiCashmere goat、223Chaidamu Cashmere goat and633Longdong Cashmere goat(1720in total)and the association of alleles with pregnancy in Hexi Cashmere Goat,using PCR-SSCP technology and by cloning and sequencing. We got the followingconclusions:(1) There were9DQA1alleles were obtained, GoLA-DQA1*B、GoLA-DQA1*E and GoLA-DQA1*H were new DQA1alleles in this study. AlleleGoLA-DQA1*F and GoLA-DQA1*H were not found in Chaidamu Cashmere goat.(2)Sequence analysis indicated that there were42single nucleotide polymorphism sites,and23amino acid polymorphism sites were found; there were observed19genotypes,GG、CC、DD is advantage genotype in Hexi Cashmere goat, G is advantage alleles;DG、DE is advantage genotype in Chaidamu Cashmere goat, D is advantage alleles;DD is advantage genotype in Longdong Cashmere goat, D is advantage alleles.(3)The univariategeneral linear model analysis showed significant statistic differencebetween different alleles (P<0.05). Frequencies of GoLA-DQA1*A were higher thanthose in normal group (P<0.01); in contrast, GoLA-DQA1*Hallelic frequencies inabortion group were lower than those in normal group (P<0.05). The result suggestedthat the alleles GoLA-DQA1*A might associated with abortion and GoLA-DQA1*Hmight related to pregnancy protection in Hexi Cashmere Goat.
引文
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