绒山羊DQA1基因第2外显子遗传特征分析
摘要
本研究以864只河西绒山羊、223只柴达木绒山羊和633只陇东绒山羊（共计1720只）为研究对象，采用PCR-SSCP和克隆测序技术分析3个山羊群体DQA1基因第2外显子的多态性和遗传特征，并进行河西绒山羊流产关联性分析。主要研究结果如下：（1）在3个山羊群体中共发现9个DQA1等位基因，其中等位基因GoLA-DQA1*B、GoLA-DQA1*E和GoLA-DQA1*H为本研究新发现。9个等位基因均存在于河西绒山羊和陇东绒山羊中，但柴达木绒山羊中未检测到等位基因GoLA-DQA1*F、GoLA-DQA1*H。（2）9个等位基因中发现42个核苷酸变异位点和23个氨基酸多态位点。河西绒山羊GG、CC、DD为优势基因型，G为优势等位基因；柴达木绒山羊DG、DE和D为优势基因型和优势等位基因；陇东绒山羊DD和D为优势基因型和优势等位基因。遗传结构分析表明3个山羊群体具有丰富的遗传多样性，且都处于Hardy-Weinberg不平衡状态。（3）经SPSS分析表明，河西绒山羊不同表型间的等位基因差异显著（P<0.05），流产组中等位基因GoLA-DQA1*A的频率高于正常组（P<0.05），等位基因GoLA-DQA1*H的频率低于正常组（P<0.01）。其余各等位基因频率的分布无差异（P>0.05）。等位基因GoLA-DQA1*A可能与流产的易感性相关，GoLA-DQA1*H可能与流产抗性有关。
With the view of studying polymorphism of DQA1gene exon2in864HexiCashmere goat、223Chaidamu Cashmere goat and633Longdong Cashmere goat(1720in total)and the association of alleles with pregnancy in Hexi Cashmere Goat,using PCR-SSCP technology and by cloning and sequencing. We got the followingconclusions:(1) There were9DQA1alleles were obtained, GoLA-DQA1*B、GoLA-DQA1*E and GoLA-DQA1*H were new DQA1alleles in this study. AlleleGoLA-DQA1*F and GoLA-DQA1*H were not found in Chaidamu Cashmere goat.(2)Sequence analysis indicated that there were42single nucleotide polymorphism sites,and23amino acid polymorphism sites were found; there were observed19genotypes,GG、CC、DD is advantage genotype in Hexi Cashmere goat, G is advantage alleles;DG、DE is advantage genotype in Chaidamu Cashmere goat, D is advantage alleles;DD is advantage genotype in Longdong Cashmere goat, D is advantage alleles.(3)The univariategeneral linear model analysis showed significant statistic differencebetween different alleles (P<0.05). Frequencies of GoLA-DQA1*A were higher thanthose in normal group (P<0.01); in contrast, GoLA-DQA1*Hallelic frequencies inabortion group were lower than those in normal group (P<0.05). The result suggestedthat the alleles GoLA-DQA1*A might associated with abortion and GoLA-DQA1*Hmight related to pregnancy protection in Hexi Cashmere Goat.
With the view of studying polymorphism of DQA1gene exon2in864HexiCashmere goat、223Chaidamu Cashmere goat and633Longdong Cashmere goat(1720in total)and the association of alleles with pregnancy in Hexi Cashmere Goat,using PCR-SSCP technology and by cloning and sequencing. We got the followingconclusions:(1) There were9DQA1alleles were obtained, GoLA-DQA1*B、GoLA-DQA1*E and GoLA-DQA1*H were new DQA1alleles in this study. AlleleGoLA-DQA1*F and GoLA-DQA1*H were not found in Chaidamu Cashmere goat.(2)Sequence analysis indicated that there were42single nucleotide polymorphism sites,and23amino acid polymorphism sites were found; there were observed19genotypes,GG、CC、DD is advantage genotype in Hexi Cashmere goat, G is advantage alleles;DG、DE is advantage genotype in Chaidamu Cashmere goat, D is advantage alleles;DD is advantage genotype in Longdong Cashmere goat, D is advantage alleles.(3)The univariategeneral linear model analysis showed significant statistic differencebetween different alleles (P<0.05). Frequencies of GoLA-DQA1*A were higher thanthose in normal group (P<0.01); in contrast, GoLA-DQA1*Hallelic frequencies inabortion group were lower than those in normal group (P<0.05). The result suggestedthat the alleles GoLA-DQA1*A might associated with abortion and GoLA-DQA1*Hmight related to pregnancy protection in Hexi Cashmere Goat.
引文
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