转Bt基因欧洲黑杨对土壤微生物的影响
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摘要
转Bt基因欧洲黑杨(Bt transgenic Populus nigra)是在1993年国家自然科学基金的资助下,由中国科学院微生物研究所与中国林业科学研究院林业研究所共同完成的一项科研成果,其表现出良好的抗虫性。Bt基因欧洲黑杨的生物安全检测结果决定了其能否进行商品化推广。转Bt基因欧洲黑杨是否对微生物系统产生影响作为生物安全检测的主要内容有着十分重要的意义。本文选择1993年中国科学院微生物研究所与中国林科院研究所共同研发成功的转Bt基因欧洲黑杨以及非转基因欧洲黑杨的1a生无性系植株为试验材料,进行了外源基因稳定性的分子检测。通过直接培养土壤微生物和将辐射杀菌的落叶干粉加入培养基后培养5种具代表性的土壤微生物这两种方法,对转Bt基因欧洲黑杨是否对微生物系统产生影响进行了评估。主要研究结果如下:
(1)Bt基因稳定存在于转Bt基因欧洲黑杨无性系植株内。
(2)转Bt基因欧洲黑杨林和非转基因林的土样中细菌数量最多,放线菌次之,真菌最少;三种微生物的数量随月份的不同而变化。
(3)转Bt基因欧洲黑杨林间和根际土壤中的三大微生物数量与非转基因林中的无显著差异,转Bt基因欧洲黑杨未对土壤微生物系统产生影响。
(4)落叶干粉的最佳辐照剂量为1kGy。
(5)转Bt基因欧洲黑杨落叶培养的5种微生物与非转基因组无显著差异,表明转Bt基因欧洲黑杨对林间土壤微生物未产生显著影响。
Research of Bt transgenic Populus nigra is funded by the national science funds in 1993. This research is an advanced achievement mend by microbial institute of the Chinese academy of sciences and forestry research institute of China's forestry science research institute. This variety had good resistance to insect sex. Biological safety testing results of Bt transgenic Populus nigra determine the doubtful for the commercialization promotion. The effects of Bt transgenic Populus nigra on soil microorganism is a very important aspect. In this paper, Bt transgenic Populus nigra and Populus nigra were elected as test materials. PCR analysis of exogenous genes was carried on. We used two methods to assessment of the influence of Bt transgenic Populus nigra on microbial system were carried on. One is cultivating soil microbial, the other one is cultivating 5 species of microbial using the medium which has the powder of fallen. The main research results are as follows:
1. The Bt gene stabled in the Bt transgenic Populus nigra.
2. In the soil of Bt transgenic Populus nigra and Populus nigra forest, the number of bacterium sum is the most, the number of fungi is the second and the number of actinomyces is the last. The numbers of the three species of microbes were changed in different months.
3. Contrasted and analyzed the microorganism numbers of transgenic group and the non- transgenic group, the results showed that the Bt transgenic Populus nigra was not impacted on the soil microbial system.
4. Appropriate irradiation dose of the powder of fallen leaves is 1kGy.
5. There were no significant differences on the five kinds of training microbes between transgenic group and the non- transgenic group. Bt transgenic Populus nigra have on influence on the forest soil microbial system.
(1)Bt基因稳定存在于转Bt基因欧洲黑杨无性系植株内。
(2)转Bt基因欧洲黑杨林和非转基因林的土样中细菌数量最多,放线菌次之,真菌最少;三种微生物的数量随月份的不同而变化。
(3)转Bt基因欧洲黑杨林间和根际土壤中的三大微生物数量与非转基因林中的无显著差异,转Bt基因欧洲黑杨未对土壤微生物系统产生影响。
(4)落叶干粉的最佳辐照剂量为1kGy。
(5)转Bt基因欧洲黑杨落叶培养的5种微生物与非转基因组无显著差异,表明转Bt基因欧洲黑杨对林间土壤微生物未产生显著影响。
Research of Bt transgenic Populus nigra is funded by the national science funds in 1993. This research is an advanced achievement mend by microbial institute of the Chinese academy of sciences and forestry research institute of China's forestry science research institute. This variety had good resistance to insect sex. Biological safety testing results of Bt transgenic Populus nigra determine the doubtful for the commercialization promotion. The effects of Bt transgenic Populus nigra on soil microorganism is a very important aspect. In this paper, Bt transgenic Populus nigra and Populus nigra were elected as test materials. PCR analysis of exogenous genes was carried on. We used two methods to assessment of the influence of Bt transgenic Populus nigra on microbial system were carried on. One is cultivating soil microbial, the other one is cultivating 5 species of microbial using the medium which has the powder of fallen. The main research results are as follows:
1. The Bt gene stabled in the Bt transgenic Populus nigra.
2. In the soil of Bt transgenic Populus nigra and Populus nigra forest, the number of bacterium sum is the most, the number of fungi is the second and the number of actinomyces is the last. The numbers of the three species of microbes were changed in different months.
3. Contrasted and analyzed the microorganism numbers of transgenic group and the non- transgenic group, the results showed that the Bt transgenic Populus nigra was not impacted on the soil microbial system.
4. Appropriate irradiation dose of the powder of fallen leaves is 1kGy.
5. There were no significant differences on the five kinds of training microbes between transgenic group and the non- transgenic group. Bt transgenic Populus nigra have on influence on the forest soil microbial system.
引文
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