胶原基生物膜的制备及性能研究
摘要
胶原是最丰富的哺乳动物蛋白质,约占体内蛋白质的20%~30%,是皮肤、软骨、动脉血管壁及结缔组织的主要成分,在动物体内起支持、保护、连接等多种作用。可制成不同形式的材料,如膜、海绵、带、薄片等。成胶材料应用于血管和伤口敷料、皮肤缺陷的弥补中。这些基质可帮助受损组织的重建。除了在生物学上的相适性外,这些材料的物理特性也与被取代的组织基本相似。
明胶是胶原降解的产物,胶原是明胶的前身,降解后的明胶仍然保持着胶原的某些构象与属性。主要由20种氨基酸所组成,其中甘氨酸占三分之一,脯氨酸与羟基脯氨酸之和占三分之一,主要组分为α_1、α_2、β以及γ三联体,微黄色,易溶于水,随温度升高或降低有溶胶与凝胶的可逆转化的优良特性,干燥后的明胶涂层呈透明状、具有一定的机械强度,在感光工业、医药工业、食品工业以及其他工业中获得非常广泛的应用。
本论文选用γ-(2,3-环氧丙氧)丙基三甲氧基硅烷(KH-560)作为交联剂对明胶膜进行交联。KH-560主要用来改善有机材料与无机材料表面的粘接性能,可提高制品的机械以及力学性能。
本论文我们还采用聚乙二醇、壳聚糖以及聚乙烯醇分别对明胶/KH-560复合膜进行改性。聚乙二醇为乙二醇与环氧乙烷的缩合物,在各种混合物中作为增湿剂来吸收和保持水分,还可用作薄膜的增塑润湿剂,可增强薄膜的强度和尺寸稳定性。壳聚糖是自然界中唯一的碱性多糖,在体内可以降解,无生物毒性及排斥反应,有很好的成形性及操作性。壳聚糖分子中含有大量的氨基,可形成Sehif碱式凝胶。聚乙烯醇是水溶性高分子聚合物,无毒,生物相容性好,可生物降解。这些材料均可明显改善明胶膜的力学性能和溶胀性能等,可望用于稳定性差的生物活性物质的包覆和作为其载体。随着组织工程的发展,无抗原性的壳聚糖与聚乙烯醇等共混物将在细胞培养、细胞外基质、骨架及生物活性物质的释放等众多方面得到应用,日益显示其在生物医学材料领域的重要性。
本文对复合膜的脆性、透光度、膨胀度、抗张强度、断裂伸长率等性能进行了表征。结果表明,聚乙二醇的使用可在一定程度上提高明胶/KH-560膜的机械性能。壳聚糖的加入引入大量的氨基、羟基和-C-O-C-基团,可与明胶的羧基反应,大大提高明胶/KH-560膜的机械性能,但是成膜后的透光度较差。聚乙烯醇不但可以提高复合膜的透明度,而且大大提高明胶/KH-560复合膜的最大负荷、断裂点负荷、伸长率、膨胀性能等。
Collagen is the most abundant mammal protein, nearly about 20%~30% in the body. It is the basis component of the skin, cartilage, artery afferent wall and knot organization, there are various function in the animal body, such as support and protection, conjunction…etc. Besides, it also can be made into the material of different form, like memrane, sponge, cingulum, thin slice etc.. The membrane can be applied into parenchyma aggrandize, blood vessel and wound dressing, skin disfigurement recuperate. Except the biologically suitability, this material can help the reconstruct of damage tissue, and its physics characteristic is also similar with the substitutional tissue.
Glutin is the outcome that the collagen disorganization, some of its contracture and property was similar with collagen. It’s composed with 20 kinds of amino acidses, among them, 1/3 was glycin, 1/3 was proline and hydroproline. Mostly element wasα_1,α_2,βandγcomplement, who easily dissolved in water, having the good characteristic that dissolved gum and gel can conver to each other along with the tempreture going up or lower, drily, presents a transparent form and has certain machine strength, acquire a very extensive application in exposed to light industry, the medicine industry, the food industry and other industry.
This thesis chooses KH-560 as to act with glutin. It is mainly used to improve organic and inorganic materials surface connect function and, can raise machine and mechanics function of ware. This thesis we still adopt Polyglycol, Chitosan and Polyvinyl alcohol to change the properties of menbrane which compounded by glutin and KH-560.Polyglycol is the polymer of glycol and ethane, is used to increase humidity and absorb moisture and to strengthen the membranes intensity and stability. The chitosan is the only alkaline amylose in the nature, can decline in the body, have good biocompatibility, low toxicity, high mechanical strength and good film-forming ability. There are many amido in the chitosan member, can fome Sehif alkali gelatin. PVA is the water solubility macromolecule polymer, which is innoxious and has a good biological adaption. These materials all can obviously improve the mechanics and dissolve property of glutine membranes. They can be made into cover materials and as the carrier of some less stability products. Along with the development of organization engineering, the polysaccharide and PVA will be used in many areas, such as cell incubate, framewoke and biological materials release, for its unantigentic,
In this paper, transport ratio, tensile strength, rupture elongation ratio were determined. The results indicated that the polyglycol can increase mechanical properties of. the membranes. Chitosan can enhance mechanical properties greatly, but the membranes transport ratio is lowest. The amido, hydroxide, and–C-O-C- groups in chitosan can act with carboxyl in glutin. PVA can enhance the membranes taansport ratio, and the other mechanical properties, such as peak load, breakpoint load, elongate rate, distensibility all are most.
明胶是胶原降解的产物,胶原是明胶的前身,降解后的明胶仍然保持着胶原的某些构象与属性。主要由20种氨基酸所组成,其中甘氨酸占三分之一,脯氨酸与羟基脯氨酸之和占三分之一,主要组分为α_1、α_2、β以及γ三联体,微黄色,易溶于水,随温度升高或降低有溶胶与凝胶的可逆转化的优良特性,干燥后的明胶涂层呈透明状、具有一定的机械强度,在感光工业、医药工业、食品工业以及其他工业中获得非常广泛的应用。
本论文选用γ-(2,3-环氧丙氧)丙基三甲氧基硅烷(KH-560)作为交联剂对明胶膜进行交联。KH-560主要用来改善有机材料与无机材料表面的粘接性能,可提高制品的机械以及力学性能。
本论文我们还采用聚乙二醇、壳聚糖以及聚乙烯醇分别对明胶/KH-560复合膜进行改性。聚乙二醇为乙二醇与环氧乙烷的缩合物,在各种混合物中作为增湿剂来吸收和保持水分,还可用作薄膜的增塑润湿剂,可增强薄膜的强度和尺寸稳定性。壳聚糖是自然界中唯一的碱性多糖,在体内可以降解,无生物毒性及排斥反应,有很好的成形性及操作性。壳聚糖分子中含有大量的氨基,可形成Sehif碱式凝胶。聚乙烯醇是水溶性高分子聚合物,无毒,生物相容性好,可生物降解。这些材料均可明显改善明胶膜的力学性能和溶胀性能等,可望用于稳定性差的生物活性物质的包覆和作为其载体。随着组织工程的发展,无抗原性的壳聚糖与聚乙烯醇等共混物将在细胞培养、细胞外基质、骨架及生物活性物质的释放等众多方面得到应用,日益显示其在生物医学材料领域的重要性。
本文对复合膜的脆性、透光度、膨胀度、抗张强度、断裂伸长率等性能进行了表征。结果表明,聚乙二醇的使用可在一定程度上提高明胶/KH-560膜的机械性能。壳聚糖的加入引入大量的氨基、羟基和-C-O-C-基团,可与明胶的羧基反应,大大提高明胶/KH-560膜的机械性能,但是成膜后的透光度较差。聚乙烯醇不但可以提高复合膜的透明度,而且大大提高明胶/KH-560复合膜的最大负荷、断裂点负荷、伸长率、膨胀性能等。
Collagen is the most abundant mammal protein, nearly about 20%~30% in the body. It is the basis component of the skin, cartilage, artery afferent wall and knot organization, there are various function in the animal body, such as support and protection, conjunction…etc. Besides, it also can be made into the material of different form, like memrane, sponge, cingulum, thin slice etc.. The membrane can be applied into parenchyma aggrandize, blood vessel and wound dressing, skin disfigurement recuperate. Except the biologically suitability, this material can help the reconstruct of damage tissue, and its physics characteristic is also similar with the substitutional tissue.
Glutin is the outcome that the collagen disorganization, some of its contracture and property was similar with collagen. It’s composed with 20 kinds of amino acidses, among them, 1/3 was glycin, 1/3 was proline and hydroproline. Mostly element wasα_1,α_2,βandγcomplement, who easily dissolved in water, having the good characteristic that dissolved gum and gel can conver to each other along with the tempreture going up or lower, drily, presents a transparent form and has certain machine strength, acquire a very extensive application in exposed to light industry, the medicine industry, the food industry and other industry.
This thesis chooses KH-560 as to act with glutin. It is mainly used to improve organic and inorganic materials surface connect function and, can raise machine and mechanics function of ware. This thesis we still adopt Polyglycol, Chitosan and Polyvinyl alcohol to change the properties of menbrane which compounded by glutin and KH-560.Polyglycol is the polymer of glycol and ethane, is used to increase humidity and absorb moisture and to strengthen the membranes intensity and stability. The chitosan is the only alkaline amylose in the nature, can decline in the body, have good biocompatibility, low toxicity, high mechanical strength and good film-forming ability. There are many amido in the chitosan member, can fome Sehif alkali gelatin. PVA is the water solubility macromolecule polymer, which is innoxious and has a good biological adaption. These materials all can obviously improve the mechanics and dissolve property of glutine membranes. They can be made into cover materials and as the carrier of some less stability products. Along with the development of organization engineering, the polysaccharide and PVA will be used in many areas, such as cell incubate, framewoke and biological materials release, for its unantigentic,
In this paper, transport ratio, tensile strength, rupture elongation ratio were determined. The results indicated that the polyglycol can increase mechanical properties of. the membranes. Chitosan can enhance mechanical properties greatly, but the membranes transport ratio is lowest. The amido, hydroxide, and–C-O-C- groups in chitosan can act with carboxyl in glutin. PVA can enhance the membranes taansport ratio, and the other mechanical properties, such as peak load, breakpoint load, elongate rate, distensibility all are most.
引文
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