养心颗粒对冠心病快速性心律失常家兔模型血浆NO含量影响的研究
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摘要
目的:通过观察以益气养血、养心安神法组成的中药复方养心颗粒对冠心病快速性心律失常家兔模型血浆一氧化氮(NO)含量的影响,探讨其防治冠心病快速性心律失常的作用机制,为指导临床应用提供依据。
方法:将28只高脂饮食喂养的家兔随机分为模型对照组、养心颗粒防治组、养心颗粒治疗组及胺碘酮组,另取7只健康家兔做为空白对照组,结合静脉注射肾上腺素的方法复制冠心病快速性心律失常模型,观察各组实验家兔心律失常出现及恢复的时间,检测各组实验家兔血脂含量的变化,采用硝酸还原酶法检测实验家兔血浆NO的含量,通过光镜和电镜观察家兔主动脉的病理形态学及超微结构的变化。
结果:养心颗粒可提高NO的含量,调节血脂的含量,明显改善家兔动脉粥样硬化的病理形态学及超微结构的异常;养心颗粒防治组、养心颗粒治疗组、胺碘酮组与模型对照组比较,快速性心律失常出现时间延迟,快速性心律失常持续时间缩短,差异有统计学意义(P＜0.05)。
养心颗粒防治组与模型对照组比较,TG、CHO、ApoB明显降低,HDL-C、ApoA明显升高,差异有统计学意义(P＜0.05);模型对照组、养心颗粒治疗组、胺碘酮组与空白对照组相比,家兔血浆NO含量降低,差异统计学意义(P＜0.05);养心颗粒防治组与模型对照组相比,家兔血浆NO含量较均显著提高,差异有统计学意义(P＜0.05)。
结论:养心颗粒可以延迟冠心病快速性心律失常出现的时间,缩短冠心病快速性心律失常的持续时间养心颗粒可以改善血脂异常,改善动脉粥样硬化的病理形态学及超微结构异常;养心颗粒能够提高家兔模型血浆NO的含量,对血管内皮功能具有保护作用,这可能是其防治冠心病快速性心律失常的作用机制之一。
Objective: Observing the effects of Yangxin granule on Plasma NO content on CHD celerity arrhythmia model , to investigate the mechanism of prevention and cure CHD celerity arrhythmia and offer science basis for clinic application.
Methods: CHD celerity arrhythmia rabbit models established by high fat and mainlining adrenalin were divided randomly into model group, Yangxin granule threat group, Yangxin granule prevention group, Amiodarone threat group. Other 7 natural rabbits were comparison group. Then observe NO and TCX、TG content.
Results: Compared with comparison group, model group , Yangxin granule group and Amiodarone thread group show that plasma NO content decreased, the different has statistics meaning (p<0.05). Compared with model group, Yangxin granule group show that plasma NO contend raised, the difference has statistics meaning (p<0.05). Compared with comparison group, model group show that appearing time of arrhythmia shorten, resuming time prolong, TC and TG obviously rise, the difference has statistics meaning. Compared with model group, Yangxin granule threat group, Yangxin granule prevention group and Amiodarone threat group show that appearing time of arrhythmia prolong, duration shorten, the difference has statistics meaning; Yangxin granule prevention group show that TC, TG decent, the difference has statistics meaning.
Conclusion: CHD celerity arrhythmia model copied by high fat and mainlining adrenalin is close to human being's arrhythmia. Yangxin granule made by the method of replenish Qi, recuperate heart could increase the synthesis of NO content thus improve endothelial cell function,this's maybe its mechanism of prevention and cure CHD celerity arrhythmia.
方法:将28只高脂饮食喂养的家兔随机分为模型对照组、养心颗粒防治组、养心颗粒治疗组及胺碘酮组,另取7只健康家兔做为空白对照组,结合静脉注射肾上腺素的方法复制冠心病快速性心律失常模型,观察各组实验家兔心律失常出现及恢复的时间,检测各组实验家兔血脂含量的变化,采用硝酸还原酶法检测实验家兔血浆NO的含量,通过光镜和电镜观察家兔主动脉的病理形态学及超微结构的变化。
结果:养心颗粒可提高NO的含量,调节血脂的含量,明显改善家兔动脉粥样硬化的病理形态学及超微结构的异常;养心颗粒防治组、养心颗粒治疗组、胺碘酮组与模型对照组比较,快速性心律失常出现时间延迟,快速性心律失常持续时间缩短,差异有统计学意义(P＜0.05)。
养心颗粒防治组与模型对照组比较,TG、CHO、ApoB明显降低,HDL-C、ApoA明显升高,差异有统计学意义(P＜0.05);模型对照组、养心颗粒治疗组、胺碘酮组与空白对照组相比,家兔血浆NO含量降低,差异统计学意义(P＜0.05);养心颗粒防治组与模型对照组相比,家兔血浆NO含量较均显著提高,差异有统计学意义(P＜0.05)。
结论:养心颗粒可以延迟冠心病快速性心律失常出现的时间,缩短冠心病快速性心律失常的持续时间养心颗粒可以改善血脂异常,改善动脉粥样硬化的病理形态学及超微结构异常;养心颗粒能够提高家兔模型血浆NO的含量,对血管内皮功能具有保护作用,这可能是其防治冠心病快速性心律失常的作用机制之一。
Objective: Observing the effects of Yangxin granule on Plasma NO content on CHD celerity arrhythmia model , to investigate the mechanism of prevention and cure CHD celerity arrhythmia and offer science basis for clinic application.
Methods: CHD celerity arrhythmia rabbit models established by high fat and mainlining adrenalin were divided randomly into model group, Yangxin granule threat group, Yangxin granule prevention group, Amiodarone threat group. Other 7 natural rabbits were comparison group. Then observe NO and TCX、TG content.
Results: Compared with comparison group, model group , Yangxin granule group and Amiodarone thread group show that plasma NO content decreased, the different has statistics meaning (p<0.05). Compared with model group, Yangxin granule group show that plasma NO contend raised, the difference has statistics meaning (p<0.05). Compared with comparison group, model group show that appearing time of arrhythmia shorten, resuming time prolong, TC and TG obviously rise, the difference has statistics meaning. Compared with model group, Yangxin granule threat group, Yangxin granule prevention group and Amiodarone threat group show that appearing time of arrhythmia prolong, duration shorten, the difference has statistics meaning; Yangxin granule prevention group show that TC, TG decent, the difference has statistics meaning.
Conclusion: CHD celerity arrhythmia model copied by high fat and mainlining adrenalin is close to human being's arrhythmia. Yangxin granule made by the method of replenish Qi, recuperate heart could increase the synthesis of NO content thus improve endothelial cell function,this's maybe its mechanism of prevention and cure CHD celerity arrhythmia.
引文
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24.尹克春,周文斌,刘淑娟等.当归Ⅱ号穴位注射治疗快速性心律失常50例[J].中西医结合心脑血管病杂志.2006,22(02):37-38.
25.刘斌.内关穴位注射苯肾上腺素、维生素B1治疗阵发性室上性心动过速疗效观察[J].中西医结合实用临床急救。1997,4(7);332.
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2.黄彩云,谢世荣,黄胜英.苦参总碱抗心律失常作用的实验研究[J].大连大学学报.2002,(4):108-110.
3.陈鼎褀.以苦参为主治疗心律失常疗效观察[J].中医杂志.1995,36(8):453.
4.巫志峰,袁小红.莲子心微囊抗心律失常作用研究[J]。中国药房.2006,17(23):1770-1771.
5.张俊红,肖军花,徐凯等.莲心总碱抗心律失常的实验研究[J].医药导报.2005,24(9):759-761.
6.肖志洁,王维芝,梁日昕.生菜籽提取物对实验性心律失常作用的初探[J].锦州医学院学报.1991,13(5)56.
7.纪相福,史道华.黄芩苷的心脑血管作用研究进展[J].海峡学.2006,18(5):10-12.
8.张志雄,祁小燕,王翔等.银杏叶提取物(EGB761)抗心律失常的实验研究[J].上海中医药杂志.2002,5(11):40-42.
9.曾靖,黄贤华,赖华等。三七总皂甙对离子通道及抗心律失常作用的研究进展[J].赣南医学院学报。2001,3(9):346-348。
10。李学军,张宝恒.三七中人参三醇甙抗心律失常作用的交感神经机理[J].中国药理学与毒理学杂志.1990,21(3):54.
11。张新荣。参松养心胶囊联合美托洛尔治疗阵发性心房颤动的临床观察[J].中外健康文摘。2008,5(2):128-129.
12.宋世飞.步长稳心颗粒与倍他乐克联用治疗心律失常的临床观察[J].实用医技杂志.2008,15(3):335-336.
13.杨思进,陈美娟,杨海涛等.心安颗粒对缺血家兔心室肌细胞电生理效应的影响[J].中国中西医结合杂志.2006,26(6):1-3。
14.郭健,席时芳,张莉.不同配比黄连肉桂煎剂抗心律失常和镇静安神作用的实验研究[J].北京中医药大学学报。2006,24(07)56-57.
15.李红,牛欣,李国彰等。调脉饮注射液抗心律失常的实验研究[J].中国中药杂志.2006,31(9):759-762.
16.郭佳,王红宁,董联玲等。舒心胶囊对大鼠实验性心律失常的影响[J].基层医学论坛。2005,9(12):1057-1058.
17.高晓东,赵健雄,刘家骏.加味保元汤注射液抗心律失常的实验研究[J].中医药学刊.2004,22(5):925-928.
18.何绪屏,陈东风,郑建宏等.健心平律丸抗心律失常的机理研究[J].广州中医药大学学报.2004,21(04):281-283。
19.徐树楠,张再康,李艳新等.增脉灵抗缓慢性心律失常的实验研究[J].中国药理学通报.2004,20(08):954-955.
20。曹湘萍,朱鹏程。穴位注射治疗冠心病的临床观察[J].中国针灸.2004。24(12);825.
21.韦泽禹,韦扬韬,韦巍.生脉注射液联合胺碘酮治疗心房纤颤30例[J].实用中医药杂志.2005,21,(6):356.
22.邹超英,朱燕文,张聘.参脉注射液治疗冠状动脉粥样硬化性心脏病心律失常疗效观察[J].中国中西医结合急救杂志.1999:6(12):572
23.徐桂芬,王文华,姚凤祯.穴位注射生脉注射液抗家兔快速性心律失常的实验研究[J].中国急救医学.2006,26(10):683-684.
24.尹克春,周文斌,刘淑娟等.当归Ⅱ号穴位注射治疗快速性心律失常50例[J].中西医结合心脑血管病杂志.2006,22(02):37-38.
25.刘斌.内关穴位注射苯肾上腺素、维生素B1治疗阵发性室上性心动过速疗效观察[J].中西医结合实用临床急救。1997,4(7);332.
26。曹广珍,王学军.穴位注射治疗心动过速 50例观察[J].穴位注射疗法临床大全.1996,12(6):341。
27.王菊光 李岩.穴位注射硫酸镁治疗小儿病毒性心肌炎心律失常78例[J].针灸临床杂志.1997,13(2);37.
28.刘三太,安康.穴位注射治疗室性心律失常 30 例[J].天津中医学院学报.1996.4:29.
29.王吉耀,廖二元,胡品津等.内科学七年制规划教材[M].第一版.人民卫生出版社.2002:196.
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