野生番茄感染叶霉菌后生化性状的变化及其抗病机制的初步研究
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摘要
Tomato leaf mold caused by ckhdosporiwn fi~Mun Cooke often takes places dining the tomato production of protective field. Tomato wild species ware used to solve this problem in tomato breeding.
This experiment was composed of four palts. Firstly, tomato leaf mold from the plastic houses of
local area was studied by aitificia] spray inoculation, consulting N. Hubbeling physiologic race differential hosts and adopting P. Day classification system. The results indicated that there ware three races in local area, including race 123,1.3 and 3. Secondly, the seedling resistance to leaf mold of eight tomato wild species were identified by inoculating with Cladosporiwnfuh4on race 12.3 that is dominant in our region. The result showed that all these tomato wild species were resistant to race 12.3. Third1~ based on this result~ we studied dynamic changes of biochemical characters (POD, PPO. relatively conductivity rate,lignin content) in leaves of these wild species inoculated after I d,3d,5d,8d, 11 d,1 5d to find biochemical mechanism of tomato wild species resistance to Cladosporiwn fidvwn . After inoculation, generally, the peroxidase (POD) or polyphenol oxidase (PPO) activities in leaves of tested tomato wild species increased significantly in comparison with those of their control plants. Relatively conductivity rate in leaves significantly increased, especially at 1st and 3rd day after inoculation. And, lignin content in tested wild species leaves after inoculation also increased than those of the control plants. Finally, transmission and scanning electron microscope were used to study leaves of tomato wild species infected by Ckidosp~riwn fidvwn. The fiinga]
gro~th on the leaves of tomato wild species was completely arrested by wild host 15 days after inoculation. Also the cell walls of leaves of tested wild species were thicker than those of susceptible variety (L402).
All these made tomato wild species resisting to Cladosporiwnfidvwn.
Postgraduate: Lv xiao-mei
Majon Vegetable Breeding
Advisot Prof. Lijing-fu
引文
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2 董汉松 植物抗病防卫基因表达调控与诱导抗性遗传的机制 植物病理学报 1996，26 (4) ：289～293
3 杜永臣等 番茄育种研究主要进展-文献综述 园艺学报 1999，26(3) ：161～169
4 冯兰香等 番茄苗期对病毒和叶霉病的多抗性鉴定方法 中国蔬菜 1994(3) ：52～55
5 高元成等 番茄野生种质资源及其利用 长江蔬菜 1994，7：3～5
6 郭兴启等 不同抗性番茄品种感染番茄花叶病毒(TMV)后若干生化反应 山东农业大 学学报 1997，28(3) 275～281
7 郭泽建 李德葆 活性氧与植物抗病性 植物学报2000，42(9) ：881～891
8 韩文华等 番茄叶霉菌形态特征鉴定及生物学特性研究 辽宁农业科学 1997 5：16～17
9 韩文华等 辽宁省番茄叶霉病菌生理小种分化研究 中国蔬菜 1999(6) ：6～9
10 何晨阳等 植物防卫反应基因的类型、表达、调控和应用 生物工程进展 1994，14(4) ： 46～50
11 何晨阳等 植物过敏反应中的生理生化变化植物生理学通讯1996，32(2) ：150～154
12 何承坤等 干旱胁迫对番茄活性氧代谢的影响 福建农业大学学报 1996，25(3) ：307～ 311
13 李柏林等 燕麦叶片衰老与活性氧代谢的关系 植物生理学报1989，15(1) ：6～12 李桂英等 东北三省番茄叶霉病生理小种分化的初步研究 东北农业大学学报 1994，25 (2) 122～125
14 李靖等 黄瓜对霜霉病抗性的显微和超显微结构的研究 武汉植物学研究 1991，9(3) 209～213
15 李靖等 黄瓜感染霜霉病菌叶片中一些酶活性的变化 植物病理学报1991，21． 4：277～ 283
16 李树德等 番茄叶霉菌生理小种的鉴定 蔬菜抗病育种进展1995 260～262，257～259
17 林义章等 水分胁迫对若干芸薹属蔬菜某些生理生化指标的影响 福建农业大学学报 1996，25(4) ：438～441
17 林植芳等 水稻叶片的衰老与超氧化物歧化酶活性及脂质过氧化作用的关系 植物学 报 1984，26(6) ：605～615
18 刘曼西 病原激发子对番茄阴离子过氧化物酶表达与反应性氧迸发的诱导 植物生理 学报 1997，23(3) ：220～226
19 刘旭 种质创新的由来与发展 作物品种资源 1999，2：1～4
20 刘晔等 番茄品种对叶霉病的抗病性鉴定结果 辽宁农业科学 1996(5) ：48～49
21 洛桂芬等 黄瓜叶片中糖和木质素含量与霜霉病诱导抗病性的关系 植物病理学报
1997，27(1) ：65～69
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