宫颈癌中MHC-I类分子抗原呈递相关蛋白GRP78、CRT、ERP57的表达及意义
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摘要
目的：探讨宫颈炎和宫颈癌中GRP78、CRT、ERP57蛋白与HPV16型感染在维吾尔族和汉族妇女中的表达及相关关系。方法：采用免疫组化S-P法和聚合酶链反应(PCR)技术检测宫颈炎69例(汉族38例,维吾尔族31例)；宫颈癌124例(汉族36例,维吾尔族88例)中GRP78、CRT、ERP57蛋白的表达和HPV16的感染情况。结果：HPV16型感染在宫颈癌中的阳性表达率(31.45%)高于宫颈炎组(11.59%),差异有统计学意义(P<0.05)。GRP78、CRT、ERP57三种蛋白在宫颈癌中的表达(95.16%,90.32%,90.32%)均高于与其各自在宫颈炎中的表达(89.96%,50.72%,49.28%),差异均有统计学意义(P<0.05)。GRP78和CRT在汉族妇女宫颈癌中的表达(100.00%,97.22%)均高于各自在汉族妇女宫颈炎中的表达(81.58%,39.47%),并且差异均有统计学意义(P<0.05)。CRT和ERP57在维吾尔族妇女宫颈癌中的表达(87.50%,94.32%)均高于各自在维吾尔族妇女宫颈炎中的表达(64.51%,35.48%),并且差异均有统计学意义(P<0.05)。除此之外,仅ERP57在维吾尔族妇女宫颈癌中的表达(94.32%)高于其在汉族宫颈癌中的表达(80.56%),差异有统计学意义(P<0.05)。而通过对GRP78、CRT、ERP57蛋白与HPV16的相关性分析发现：GRP78、CRT、ERP57蛋白在维吾尔族妇女宫颈癌中的阳性表达率与HPV16型感染均存在正相关关系(r=0.278 P<0.05；r=0.429 P<0.05；r=0.222P<0.05)。CRT与GRP78、ERP57蛋白在维吾尔族妇女宫颈癌中的表达均存在正相关关系(r=0.579 P<0.05；r=0.353 P<0.05)。结论：通过对GRP78、CRT、ERP57三种蛋白和HPV16联合检测,可能作为检查宫颈癌的标记物,提高宫颈癌的检出率；ERP57蛋白在宫颈癌中的表达存在民族间差异。
Objective:To evaluate the expression levels of the MHC class I antigen presentation associated proteins GRP78、CRT and ERP57 and their association with HPV16 infection in human cervical cancer lesions from Uygur and Han patients. Methods:The expression of proteins GRP78、CRT and ERP57 in 69 cervicitis tissues including 38 Han and 31 Uygur women and 124 cervical cancer lesions from 36 Han and 88 Uygur women were evaluated by S-P immunohistochemistry, and the infection status of HPV in these tissues was studied by PCR using specific primers for HPV16. Results:The infection rate of HPV 16 in cervical cancer was higher than that in cervicitis tissues,40.91%,8.33% respectively, and the difference was significant (P<0.05). GRP78、CRT and ERP57 in cervical cancer (95.16%,90.32%,90.32%) from both ethnic groups were significantly differences (P<0.05), compared with that in cervicitis tissues(89.96%,50.72%,49.28%) respectively. The expression rates of both GRP78 and CRT in cervicitis from Han women (93.18%,87.50%) were significantly reduced when compared to that in cervical cancer(100.00%,97.22%) from Han women, and the differences had statistical significance yet(P<0.05). The expression rates of both CRT and ERP57 in cervicitis from Uygur women (64.51%,35.48%) were significantly reduced when compared to that in cervical cancer(87.50%,94.32%) from Uygur women, and the differences had statistical significance yet(P<0.05). The overall expression rates of Besides, there were significantly differences(P<0.05) in expression of ERP57 in cervical cancer tissues between Han (80.56%)and Uygur women(94.32%).There were positive correlation in expression of CRT、ERP57、GRP78 and HPV in cervical cancers of Uygur women(r=0.429 P<0.05; r=0.222 P<0.05; r=0.278 P<0.05).There were positive correlation in expression of CRT and GRP78、ERP57in cervical cancers of Uygur women(r=0.579 P<0.05; r=0.353 P<0.05).
Conclusion:The detection of GRP78、CRT and ERP57 besides HPV16 may improve diagnosis of cervical cancer. The expressions of ERP57 was significant differences in cervical cancer of ethnic group boths.
Objective:To evaluate the expression levels of the MHC class I antigen presentation associated proteins GRP78、CRT and ERP57 and their association with HPV16 infection in human cervical cancer lesions from Uygur and Han patients. Methods:The expression of proteins GRP78、CRT and ERP57 in 69 cervicitis tissues including 38 Han and 31 Uygur women and 124 cervical cancer lesions from 36 Han and 88 Uygur women were evaluated by S-P immunohistochemistry, and the infection status of HPV in these tissues was studied by PCR using specific primers for HPV16. Results:The infection rate of HPV 16 in cervical cancer was higher than that in cervicitis tissues,40.91%,8.33% respectively, and the difference was significant (P<0.05). GRP78、CRT and ERP57 in cervical cancer (95.16%,90.32%,90.32%) from both ethnic groups were significantly differences (P<0.05), compared with that in cervicitis tissues(89.96%,50.72%,49.28%) respectively. The expression rates of both GRP78 and CRT in cervicitis from Han women (93.18%,87.50%) were significantly reduced when compared to that in cervical cancer(100.00%,97.22%) from Han women, and the differences had statistical significance yet(P<0.05). The expression rates of both CRT and ERP57 in cervicitis from Uygur women (64.51%,35.48%) were significantly reduced when compared to that in cervical cancer(87.50%,94.32%) from Uygur women, and the differences had statistical significance yet(P<0.05). The overall expression rates of Besides, there were significantly differences(P<0.05) in expression of ERP57 in cervical cancer tissues between Han (80.56%)and Uygur women(94.32%).There were positive correlation in expression of CRT、ERP57、GRP78 and HPV in cervical cancers of Uygur women(r=0.429 P<0.05; r=0.222 P<0.05; r=0.278 P<0.05).There were positive correlation in expression of CRT and GRP78、ERP57in cervical cancers of Uygur women(r=0.579 P<0.05; r=0.353 P<0.05).
Conclusion:The detection of GRP78、CRT and ERP57 besides HPV16 may improve diagnosis of cervical cancer. The expressions of ERP57 was significant differences in cervical cancer of ethnic group boths.
引文
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