川木香的化学成分及质量标准的初步研究
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摘要
川木香为菊科川木香属植物川木香Vladimiria souliei(Franch.)Ling.及灰毛川木香Vladimiria souliei(Franch.)Ling vat.cinerea Ling.的干燥根。曾一度被作为为中药木香的地方习用、代用品,1963年开始被《中国药典》单独收载,直到2005年。主产于四川地区,为中医常用行气止痛药,同时也是藏族、羌族、蒙古等族习用药,主要用于消化道疾病。历史上木香类药材品种众多,名称几经变化,导致现今临床运用混乱。本课题从川木香本草考证出发,研究了川木香药材的来源、功效、炮制及现代研究,对其进行了化学成分研究。继而在化学成分研究结果的基础上进行了其标志性成分木香烃内酯和去氢木香内酯,以及分离得到的三个化合物的含量测定;并通过色谱指纹图谱,初步建立了川木香药材的质量标准。
1木香类药材的本草考证
通过查阅数量众多的古代文献,对木香类药材的品种和名称的历史变迁进行了完善的考察和整理,理清各种代用品、混淆品之间的关系。
2川木香的化学成分研究
采用硅胶柱层析方法,对川木香醇水双提物的乙酸乙酯部位进行了化学成分研究,共分离得到11个化合物,分别为α-amyrin margarate(Ⅰ)、Isobauerenyl acetate(异降香萜烯酸乙酸酯)(Ⅱ)、Dehydrocostus lactone(去氢木香内酯)(Ⅲ)、Uvaol(熊果醇)(Ⅳ)、15-Acetoxycostunolide(15-乙酰氧基木香烯内酯)(Ⅴ)、Santamarine(珊塔玛内酯)(Ⅵ)、Prinsepiol(青刺尖木脂醇)(Ⅶ)、Syringaresinol(丁香脂素)(Ⅷ)、VladinolD(川木香醇D)(Ⅸ)、Costunolide(木香烃内酯)(Ⅹ)、Cynaropicrin(菜蓟苦素;洋蓟内酯)(Ⅺ)。除去木香烃内酯、去氢木香内酯及川木香醇D为已知化合物外,其余均为初次在川木香药材中发现。
3川木香生品及煨品的质量标准研究
通过高效液相色谱法,用木香烃内酯、去氢木香内酯以及化学成分研究分离得到的3种化合物(化合物Ⅱ、Ⅷ、Ⅺ)作为对照品,对川木香进行了含量测定。分析结果不同产地川木香在此5个化合物含量上有一定区别;采用高效液相色谱法,以木香烃内酯为指标,对川木香药材进行了指纹图谱研究。
Radix Dolomiaeae is the dried rhizome of Vladimiria souliei(Franch.)Ling.and Vladimiria souliei(Franch.)Ling vat.cinerea Ling.It was used as a succedaneum of Radix Aucklandiae for a long time.1963,began to be“chinese pharmacopoeia”singal collection until 2005. It is mainly produced in Sichuan.It is uses to relieving Qi and relieve pain as Chinese traditional medicine,but also Tibetan, Qiang, Mongolia, habitual medicine, mainly for digestive diseases.There were many varieties of plants which were used as Mu-xiang in history,and the name changed several times,leading to the Clinical application of chaos. From the textual research of materia medica,members of this project investigated the resources、effect、processing,carried out its chemical constituents.Then assayed Costunolide、Dehydrocostus lactone and other three compounds which obtained by ourself by HPLC. Initially established its qu-ality standards by chromatographic fingerprint.
1 materia medica herbal textual research of Mu-xiang and
Through access to a large number of ancient literature,we did complete inspection and sorting on its species and names of the historical changes. Clarified the relationships between the various alternatives and the confused.
2 Chemical constituents of Radix Dolomiaeae
Silica gel column chromatography was used to analysis Radix Dolomiaeae.We got 11 compounds from it ,α-amyrin margarate(Ⅰ)、Isobauerenyl acetate(Ⅱ)、Dehydrocostus lactone(Ⅲ)、Uvaol(Ⅳ)、15-Acetoxycostunolide(Ⅴ)、Santamarine(Ⅵ)、Prinsepiol(Ⅶ)、Syringaresinol(Ⅷ)、VladinolD(Ⅸ)、Costunolide(Ⅹ)and Cynaropicrin(Ⅺ)。except Costunolide、VladinolD and Dehydrocostus lactone are known active ingredients,others are found in Radix Dolomiaeae the first time.
3 Quality Standards of Radix Dolomiaeae
By using the method of HPLC to determine the content of Dehydrocostus lactone、Costunolide、bauerenyl acetate、Syringaresinol and Cynaropicrin.The results showed that there are certain distinctions between the content of these compounds. By using the method of HPLC, cost-unolide be the index , we conducted fingerprint on Radix Dolomiaeae, established its quality standards initially.
1木香类药材的本草考证
通过查阅数量众多的古代文献,对木香类药材的品种和名称的历史变迁进行了完善的考察和整理,理清各种代用品、混淆品之间的关系。
2川木香的化学成分研究
采用硅胶柱层析方法,对川木香醇水双提物的乙酸乙酯部位进行了化学成分研究,共分离得到11个化合物,分别为α-amyrin margarate(Ⅰ)、Isobauerenyl acetate(异降香萜烯酸乙酸酯)(Ⅱ)、Dehydrocostus lactone(去氢木香内酯)(Ⅲ)、Uvaol(熊果醇)(Ⅳ)、15-Acetoxycostunolide(15-乙酰氧基木香烯内酯)(Ⅴ)、Santamarine(珊塔玛内酯)(Ⅵ)、Prinsepiol(青刺尖木脂醇)(Ⅶ)、Syringaresinol(丁香脂素)(Ⅷ)、VladinolD(川木香醇D)(Ⅸ)、Costunolide(木香烃内酯)(Ⅹ)、Cynaropicrin(菜蓟苦素;洋蓟内酯)(Ⅺ)。除去木香烃内酯、去氢木香内酯及川木香醇D为已知化合物外,其余均为初次在川木香药材中发现。
3川木香生品及煨品的质量标准研究
通过高效液相色谱法,用木香烃内酯、去氢木香内酯以及化学成分研究分离得到的3种化合物(化合物Ⅱ、Ⅷ、Ⅺ)作为对照品,对川木香进行了含量测定。分析结果不同产地川木香在此5个化合物含量上有一定区别;采用高效液相色谱法,以木香烃内酯为指标,对川木香药材进行了指纹图谱研究。
Radix Dolomiaeae is the dried rhizome of Vladimiria souliei(Franch.)Ling.and Vladimiria souliei(Franch.)Ling vat.cinerea Ling.It was used as a succedaneum of Radix Aucklandiae for a long time.1963,began to be“chinese pharmacopoeia”singal collection until 2005. It is mainly produced in Sichuan.It is uses to relieving Qi and relieve pain as Chinese traditional medicine,but also Tibetan, Qiang, Mongolia, habitual medicine, mainly for digestive diseases.There were many varieties of plants which were used as Mu-xiang in history,and the name changed several times,leading to the Clinical application of chaos. From the textual research of materia medica,members of this project investigated the resources、effect、processing,carried out its chemical constituents.Then assayed Costunolide、Dehydrocostus lactone and other three compounds which obtained by ourself by HPLC. Initially established its qu-ality standards by chromatographic fingerprint.
1 materia medica herbal textual research of Mu-xiang and
Through access to a large number of ancient literature,we did complete inspection and sorting on its species and names of the historical changes. Clarified the relationships between the various alternatives and the confused.
2 Chemical constituents of Radix Dolomiaeae
Silica gel column chromatography was used to analysis Radix Dolomiaeae.We got 11 compounds from it ,α-amyrin margarate(Ⅰ)、Isobauerenyl acetate(Ⅱ)、Dehydrocostus lactone(Ⅲ)、Uvaol(Ⅳ)、15-Acetoxycostunolide(Ⅴ)、Santamarine(Ⅵ)、Prinsepiol(Ⅶ)、Syringaresinol(Ⅷ)、VladinolD(Ⅸ)、Costunolide(Ⅹ)and Cynaropicrin(Ⅺ)。except Costunolide、VladinolD and Dehydrocostus lactone are known active ingredients,others are found in Radix Dolomiaeae the first time.
3 Quality Standards of Radix Dolomiaeae
By using the method of HPLC to determine the content of Dehydrocostus lactone、Costunolide、bauerenyl acetate、Syringaresinol and Cynaropicrin.The results showed that there are certain distinctions between the content of these compounds. By using the method of HPLC, cost-unolide be the index , we conducted fingerprint on Radix Dolomiaeae, established its quality standards initially.
引文
[1]TaD-RX,Jakupovic J,Bohlmann F,el a1.Sesquiterpenelactones frornVladimiriasouliei[J].Pkytockemistry,1990,29(4):1 209
[2]李兆琳.川I木香挥发油化学成分的研究[J].兰州大学学报(自然科学版),1991,27(4):94.
[3]黄好武,施文科,梁晟.川木香挥发性成分的气相色谱-质谱分析[J].中国医院用药评价与分析.2008,8(9):675-676.
[4]蒋淼,王家葵.青木香名实小考[J].四川省卫生管理干部学院.2009,28(2):162-163.
[5][日]小西天二.土木香中抑制肿瘤癌细胞增殖的成分[J].国外医学中医中药分册.2003,25(3):174.
[6]赖先荣,曾桢,许凌霞.藏药布嘎木拉(川木香)研究进展[J].2004全国藏医药学术讨论会论文集:506.
[7]Copeland BJ,NeffNH,Hadjiconstantinou M.Enhanced dopamine up—take in the striatum following repeated restraint stress[J]_Synapse,2005,57:167—174
[8]David L,Walker Kerry J,Ressler Kwak—Tung LU,et a1.Facilitation of conditioned fear exinction by systemic administration or in~a-amygdala infusions ofD—Cycloserine as assessed with fear-potentiatedstart1e in rats[J].The Joumal ofNeuroscience,2002,22(6):2343—2351
[9] J. Chin. Triterpenoid from viburnum odoratissimum[J].Chem. Soc. 2003, 50(2), 298-230
[10]刘文粢,何风雷,阮子镛等.狼毒大戟的化学成分研究[J].中国中药杂志2001,26(3):180-182.
[11]A.K.Chakravarty et al.Peracid oxidation products of swertanone[J].Tetraledron.1991,47(1213):2337～2350.
[12]杨辉,谢金轮,孙汉董.云木香化学成分研究[J].云南植物研究.1997,19(1):85～91.
[13] S.Siddiqui et al.Kaneric acid ,a new triterpene from the leaves of Nerium Oleander[J].Journal of Natural Products.1986,49(6):1086~1090.
[14]R.X.Tan et al.Sesquiterpene lactones from Vladimiria Souliei[J].Phytochemistry.1990,29(4):1209～1212.
[15]Piccinelli et al.New Lignans from the Roots of Valeriana prionophylla with Antioxidative and Vasorelaxant Activities [J].Journal of Natural Products.2004,67(7):1134～1137.
[16]Zuo G Y,He H P,Hong X,el a1.Chemical constituents ofSpiraea japonica var.ovalifoh'a [J].Acta Bot Yunnan(云南植物研究),2005,27:101—106.
[17]Fumiko Abe and Tatsuo Yamauchi. 9α-Hydroxypinoresinol,9α-Hydroxymedioresinol and related lignans from Allamanda Neriifolia J].Phytochemistry[.1988,27(2):575～577.
[18]付朝晖,张玉梅,谭宁华等.云南油杉的化学成分研究[J].天然产物研究与开发.2008,20:257～261.
[19]I.Muhammad et al.Cytotoxic Sesquiterpene Lactones from Centaurothamnus maximus and Vicoa pentanema[J]. Phytotherapy Research, 2003, 17(2), 168-173.
[20]彭镰心,刘圆,孙卓然等.RP-HPLC法测定木香与川木香中木香烃内酯的含量[J].西南民族大学学报(自然科学版).2007,33(2):337-339.
[21]王永兵,许华,王强.RP-HPLC法测定川木香中木香烃内酯和去氢木香内酯的含量[J].西北药学杂志.2000,15(6):250-251.
[22]李蓉涛,韩全斌,郑永唐等.小花五味子中抗HIV活性的木脂素类化合物[J].中国天然药物.2005,3(4):212~213.
[23] Cho JY, Park J, Yoo ES, et al·Inhibitory effect of sesquiterpene lactones from saussurea lappa on tumor necrosis factoralpha production in murine macrophagelike cells[J]·Planta Med·, 1998, 64(7): 594~597·
[24] Cho Jae Youl,Park Jisoo ,Yoo Euri Sook,et al.Inhibitory effect of sesquiterpene lactone fromSaussurea lappaon tumor necrosis factor-αproduction in murine macrophage like cells[J].Plant Med,1998,64(7):594.
[25][日]松田久司等.洋蓟叶中抑制血脂升高的成分.国外医学中医中药分册.2003,25(3):173.
[26]林萍,毕志明,徐红等.石斛属植物药理活性研究进展[J].中草药.2003,34(11):附19~附22.
[1]赖先荣,曾桢,许凌霞.藏药布嘎木拉(川木香)研究进展.2004全国藏医药学术讨论会论文集:506
[2]彭镰心,刘圆,孙卓然等.RP-HPLC法测定木香与川木香中木香烃内酯的含量.西南民族大学学报(自然科学版).2007,33(2):337-339
[3]李兆琳,薛敦渊,王明奎等.川木香挥发油化学成分的研究.兰州大学学报(自然科学版).1991,27(4):94-97.
[4]黄好武,施文科,梁晟.川木香挥发性成分的气相色谱-质谱分析.中国医院用药评价与分析.2008,8(9):675-676.
[5]赖先荣,孟保华,江志尧等.川木香对实验性溃疡形成的抑制作用研究.现代生物医学进展.2008,8(1):34-36
[6]于浴生主编.中药药理与应用[M].北京:人民卫生出版社,1983:170、171.
[7]王永兵,王强.木香的药效学研究[J].中国药科大学学报,2001,32(2):146.
[8]李秀芳,林青,代蓉.云木香丙酮提取物对大鼠实验性溃疡模型的影响.云南中医中药杂志.2007,28(6):34-35.
[9]杨辉,谢金伦,孙汉董·云木香化学成分及药理作用研究概况[J]·天然产物研究与开发,1998,10(2)∶90~98.
[1]谢培山.现代化中药产业关键技术系列研讨会(一)国际色谱指纹图评价中药质量研讨会学术报告论文集[C].广州, 2001. i3-1,i3-27.
[2]罗国安,王义明.中药指纹图谱的分类和发展[J].中国新药杂志.2002,11(1):46~51.
[3]高晓霞,严寒静,梁从庆,等.何首乌药材的薄层色谱指纹图谱质量评价初步研究.中药材,2007,30:407-409.
[4]林明美,罗燕.药物分析杂志,1987,7(l):13
[5]张恩景,张晓燕,李宏亮等.薜荔果药材中黄酮类成分的指纹图谱研究[J].医药导报.2009,28(11):1507~1509.
[6]李贺敏,周艳.不同产地白花蛇舌草的高效液相色谱指纹图谱分析[J].河南农业大学学报.2009,43(6):596~600.
[7]姜勇,张娜,崔振等.远志药材的HPLC指纹图谱[J].药学学报.2006,41(2):179~183.
[8]周婧,都晓伟,马宏跃等.西洋参高效液相色谱法指纹图谱的研究[J].时珍国医国药.2006,17(12):2381~2382.
[9]张峰,付绍平,徐青等.杜衡GC指纹图谱的初步研究[J].现代中药研究与实践.2004,18(增刊):21~23.
[10]万明珠梁逸曾,赵晨曦等.温莪术挥发性化学成分分析和指纹图谱研究[J].时珍国医国药.2009,20(10):2428~2430.
[11]王世勇,宋力飞,黄晓玲等.郁金的指纹图谱研究[J].中药新药与临床药理,2004,15(6):406~409.
[12]王秀坤,李家实,魏璐雪等.苦参质量的化学模式识别[J].中国中药杂志.1996,21(4):198.
[13]李莹,孙卓然,刘圆等.不同种石斛的红外指纹图谱研究[J].西南民族大学学报(自然科学版).2009,35(5):1024~1027.
[14]王秀文,刘养清,王庚等.六神丸红外指纹图谱的研究[J].山西中医学院学报.2009,10(3):23~25.
[15]孙国祥,王真,金杰等.中药红外光谱指纹图谱专家网络系统(TCM-IRFP-ESG)构建与应用[J].中南药学.2009,7(8):622~625
[16]孙国祥,孙毓庆,王宇.复方甘草片的毛细管电泳指纹图谱研究[J].中南药学.2003,1(3):131~134.
[17]孙沂,隋因,郭涛等.不同产地红花药材的高效毛细管电泳指纹图谱研究[J].中国药学杂志.2003,38(3):176~178.
[18]吴惠勤,黄晓兰,林晓珊等.白豆蔻挥发油GC-MS指纹图谱研究[J].中药材.2006,29(8):788~792.
[19]孙国祥,胡玥珊,金杰等.中药X射线衍射指纹图谱专家网络系统网格(TCM-XFP-ESG)构建与应用[J].中南药学.2009,7(10):766~769.
[20]王树春,吕扬,昊楠,等.中草药,2000,31(3):214
[21]孙庆雷,赵红霞,林云良等.黄芩的1HNMR指纹图谱研究[J].波普学杂志.2007,24(2):163~168.
[22]宁井铭,张正竹,方世辉等.指纹图谱技术及其在茶叶品质控制中的应用[J].中国茶叶加工.2009,(3):39~41.
[23]黎洪利,赵朋贤,杨俊等.紫外指纹图谱在烟用香精质量控制中的应用[J].中国烟草学报.2009,15(4):19~21.
[2]李兆琳.川I木香挥发油化学成分的研究[J].兰州大学学报(自然科学版),1991,27(4):94.
[3]黄好武,施文科,梁晟.川木香挥发性成分的气相色谱-质谱分析[J].中国医院用药评价与分析.2008,8(9):675-676.
[4]蒋淼,王家葵.青木香名实小考[J].四川省卫生管理干部学院.2009,28(2):162-163.
[5][日]小西天二.土木香中抑制肿瘤癌细胞增殖的成分[J].国外医学中医中药分册.2003,25(3):174.
[6]赖先荣,曾桢,许凌霞.藏药布嘎木拉(川木香)研究进展[J].2004全国藏医药学术讨论会论文集:506.
[7]Copeland BJ,NeffNH,Hadjiconstantinou M.Enhanced dopamine up—take in the striatum following repeated restraint stress[J]_Synapse,2005,57:167—174
[8]David L,Walker Kerry J,Ressler Kwak—Tung LU,et a1.Facilitation of conditioned fear exinction by systemic administration or in~a-amygdala infusions ofD—Cycloserine as assessed with fear-potentiatedstart1e in rats[J].The Joumal ofNeuroscience,2002,22(6):2343—2351
[9] J. Chin. Triterpenoid from viburnum odoratissimum[J].Chem. Soc. 2003, 50(2), 298-230
[10]刘文粢,何风雷,阮子镛等.狼毒大戟的化学成分研究[J].中国中药杂志2001,26(3):180-182.
[11]A.K.Chakravarty et al.Peracid oxidation products of swertanone[J].Tetraledron.1991,47(1213):2337～2350.
[12]杨辉,谢金轮,孙汉董.云木香化学成分研究[J].云南植物研究.1997,19(1):85～91.
[13] S.Siddiqui et al.Kaneric acid ,a new triterpene from the leaves of Nerium Oleander[J].Journal of Natural Products.1986,49(6):1086~1090.
[14]R.X.Tan et al.Sesquiterpene lactones from Vladimiria Souliei[J].Phytochemistry.1990,29(4):1209～1212.
[15]Piccinelli et al.New Lignans from the Roots of Valeriana prionophylla with Antioxidative and Vasorelaxant Activities [J].Journal of Natural Products.2004,67(7):1134～1137.
[16]Zuo G Y,He H P,Hong X,el a1.Chemical constituents ofSpiraea japonica var.ovalifoh'a [J].Acta Bot Yunnan(云南植物研究),2005,27:101—106.
[17]Fumiko Abe and Tatsuo Yamauchi. 9α-Hydroxypinoresinol,9α-Hydroxymedioresinol and related lignans from Allamanda Neriifolia J].Phytochemistry[.1988,27(2):575～577.
[18]付朝晖,张玉梅,谭宁华等.云南油杉的化学成分研究[J].天然产物研究与开发.2008,20:257～261.
[19]I.Muhammad et al.Cytotoxic Sesquiterpene Lactones from Centaurothamnus maximus and Vicoa pentanema[J]. Phytotherapy Research, 2003, 17(2), 168-173.
[20]彭镰心,刘圆,孙卓然等.RP-HPLC法测定木香与川木香中木香烃内酯的含量[J].西南民族大学学报(自然科学版).2007,33(2):337-339.
[21]王永兵,许华,王强.RP-HPLC法测定川木香中木香烃内酯和去氢木香内酯的含量[J].西北药学杂志.2000,15(6):250-251.
[22]李蓉涛,韩全斌,郑永唐等.小花五味子中抗HIV活性的木脂素类化合物[J].中国天然药物.2005,3(4):212~213.
[23] Cho JY, Park J, Yoo ES, et al·Inhibitory effect of sesquiterpene lactones from saussurea lappa on tumor necrosis factoralpha production in murine macrophagelike cells[J]·Planta Med·, 1998, 64(7): 594~597·
[24] Cho Jae Youl,Park Jisoo ,Yoo Euri Sook,et al.Inhibitory effect of sesquiterpene lactone fromSaussurea lappaon tumor necrosis factor-αproduction in murine macrophage like cells[J].Plant Med,1998,64(7):594.
[25][日]松田久司等.洋蓟叶中抑制血脂升高的成分.国外医学中医中药分册.2003,25(3):173.
[26]林萍,毕志明,徐红等.石斛属植物药理活性研究进展[J].中草药.2003,34(11):附19~附22.
[1]赖先荣,曾桢,许凌霞.藏药布嘎木拉(川木香)研究进展.2004全国藏医药学术讨论会论文集:506
[2]彭镰心,刘圆,孙卓然等.RP-HPLC法测定木香与川木香中木香烃内酯的含量.西南民族大学学报(自然科学版).2007,33(2):337-339
[3]李兆琳,薛敦渊,王明奎等.川木香挥发油化学成分的研究.兰州大学学报(自然科学版).1991,27(4):94-97.
[4]黄好武,施文科,梁晟.川木香挥发性成分的气相色谱-质谱分析.中国医院用药评价与分析.2008,8(9):675-676.
[5]赖先荣,孟保华,江志尧等.川木香对实验性溃疡形成的抑制作用研究.现代生物医学进展.2008,8(1):34-36
[6]于浴生主编.中药药理与应用[M].北京:人民卫生出版社,1983:170、171.
[7]王永兵,王强.木香的药效学研究[J].中国药科大学学报,2001,32(2):146.
[8]李秀芳,林青,代蓉.云木香丙酮提取物对大鼠实验性溃疡模型的影响.云南中医中药杂志.2007,28(6):34-35.
[9]杨辉,谢金伦,孙汉董·云木香化学成分及药理作用研究概况[J]·天然产物研究与开发,1998,10(2)∶90~98.
[1]谢培山.现代化中药产业关键技术系列研讨会(一)国际色谱指纹图评价中药质量研讨会学术报告论文集[C].广州, 2001. i3-1,i3-27.
[2]罗国安,王义明.中药指纹图谱的分类和发展[J].中国新药杂志.2002,11(1):46~51.
[3]高晓霞,严寒静,梁从庆,等.何首乌药材的薄层色谱指纹图谱质量评价初步研究.中药材,2007,30:407-409.
[4]林明美,罗燕.药物分析杂志,1987,7(l):13
[5]张恩景,张晓燕,李宏亮等.薜荔果药材中黄酮类成分的指纹图谱研究[J].医药导报.2009,28(11):1507~1509.
[6]李贺敏,周艳.不同产地白花蛇舌草的高效液相色谱指纹图谱分析[J].河南农业大学学报.2009,43(6):596~600.
[7]姜勇,张娜,崔振等.远志药材的HPLC指纹图谱[J].药学学报.2006,41(2):179~183.
[8]周婧,都晓伟,马宏跃等.西洋参高效液相色谱法指纹图谱的研究[J].时珍国医国药.2006,17(12):2381~2382.
[9]张峰,付绍平,徐青等.杜衡GC指纹图谱的初步研究[J].现代中药研究与实践.2004,18(增刊):21~23.
[10]万明珠梁逸曾,赵晨曦等.温莪术挥发性化学成分分析和指纹图谱研究[J].时珍国医国药.2009,20(10):2428~2430.
[11]王世勇,宋力飞,黄晓玲等.郁金的指纹图谱研究[J].中药新药与临床药理,2004,15(6):406~409.
[12]王秀坤,李家实,魏璐雪等.苦参质量的化学模式识别[J].中国中药杂志.1996,21(4):198.
[13]李莹,孙卓然,刘圆等.不同种石斛的红外指纹图谱研究[J].西南民族大学学报(自然科学版).2009,35(5):1024~1027.
[14]王秀文,刘养清,王庚等.六神丸红外指纹图谱的研究[J].山西中医学院学报.2009,10(3):23~25.
[15]孙国祥,王真,金杰等.中药红外光谱指纹图谱专家网络系统(TCM-IRFP-ESG)构建与应用[J].中南药学.2009,7(8):622~625
[16]孙国祥,孙毓庆,王宇.复方甘草片的毛细管电泳指纹图谱研究[J].中南药学.2003,1(3):131~134.
[17]孙沂,隋因,郭涛等.不同产地红花药材的高效毛细管电泳指纹图谱研究[J].中国药学杂志.2003,38(3):176~178.
[18]吴惠勤,黄晓兰,林晓珊等.白豆蔻挥发油GC-MS指纹图谱研究[J].中药材.2006,29(8):788~792.
[19]孙国祥,胡玥珊,金杰等.中药X射线衍射指纹图谱专家网络系统网格(TCM-XFP-ESG)构建与应用[J].中南药学.2009,7(10):766~769.
[20]王树春,吕扬,昊楠,等.中草药,2000,31(3):214
[21]孙庆雷,赵红霞,林云良等.黄芩的1HNMR指纹图谱研究[J].波普学杂志.2007,24(2):163~168.
[22]宁井铭,张正竹,方世辉等.指纹图谱技术及其在茶叶品质控制中的应用[J].中国茶叶加工.2009,(3):39~41.
[23]黎洪利,赵朋贤,杨俊等.紫外指纹图谱在烟用香精质量控制中的应用[J].中国烟草学报.2009,15(4):19~21.