薄荷品种资源遗传多样性研究及优异种质评价
摘要
薄荷为唇形科薄荷属中的一类重要植物,其所含的薄荷精油具有十分重要的经济价值广泛应用于食品、医药及日化产品领域。本研究对所收集的薄荷品种资源分别从叶片性状,生物量、精油产量、精油成分等方面进行了分析评价,同时利用化学和分子标(?)(Amplified Fragment Length Polymorphism and chloroplast DNA)分别对其进行化学多样性和分子遗传多样性分析。主要研究结果如下：
1.薄荷叶片性状及精油产量评价。对薄荷叶片性状变异分析表明,除叶长宽比的变异系数较低外,其余各性状的变异系数均在35%以上,其中M×gracilis-01的叶片重量最大为0.15g,M. aquatica-01的叶长宽比最大为2.5,M. spicata-06的叶面积最大为7.8cm2,M.spicata-02的油腺数量最多为8475个。对精油含量、鲜草产量和精油产量变异分析表明,除鲜草产量的变异系数较低外,精油含量和精油产量的变异系数均在50%以上,其中M. spicata-08的鲜草亩产量最高为1467.4kg,M.canadensis-02的精油含量和精油亩产量最高分别为0.92%和10.3kg。相关性分析显示叶面积与精油产量、精油含量和叶长宽比之间显著正相关,与油腺数量呈现极显著正相关；精油产量与精油含量呈现极显著正相关。对薄荷精油产量进行综合评价显示精油亩产量在6.0kg以上的薄荷资源有M. canadensis-02等10种。结果表明所收集的薄荷品种在叶片性状和精油产量方面存在丰富的变异,在一些重要性状之间存在极显著的相关性。
2.薄荷精油成分研究及评价。精油成分分析结果表明,薄荷精油成分呈现多样性,其中相对含量在5.0%以上有menthol等27种精油成分。不同花期精油成分分析结果表明,精油成分相对含量在不同花期存在差异性变化,其中globulol等7种精油成分相对含量在初花期最高。menthol等16种精油成分相对含量在盛花期最高。limonene等4种精油成分相对含量在末花期最高。脱落酸处理对薄荷精油成分影响的分析结果表明,经脱落酸处理后menthofuran含量显著提高导致精油品质下降。脱落酸处理后的薄荷精油主成分相对含量间的相关性分析表明,薄荷精油各主成分相对含量间存在动态平衡,变化规律符合线性方程menthol=74.787-1.044×menthone-0.705×menthofuran-0.796×pulegonec脱落酸处理后的薄荷精油合成相关基因表达分析结果表明,ABA处理会显著影响薄荷精油合成相关基因的表达。精油成分在不同薄荷资源间存在差异性,这些差异是影响精油品质的主要因素,依据精油成分评价,具有生产高品质薄荷精油潜力的种质资源有M. spicata-08等10种。
3.薄荷资源chloroplast DNA亲缘关系分析。所收集的薄荷品种资源共产生了两个主要的聚类,聚类I包括M. spicata-M. suaveolens-M. longifolia-M.×gracilis-M. arvensis-M×piperita等薄荷群体,聚类II包括M. aquatica-M. xpiperita-M.×piperita等薄荷群体。其中聚类1分为3个亚组:①M. spicata-M. longifolia、②M. spicata-M. suaveolens和③M.arvensis-M. xgracilis,聚类11分为2个亚组：①M. canadensis-M.aquatica和②M.×piperita。结果表明,亲缘关系相近的薄荷群体聚为同一类,同时本研究结果清晰的反映了母本与其后代之间的亲缘关系。
4.薄荷资源精油化学型分析。M. spicata群体精油包含2个化学型：limonene-carvone型和1,8-cineole-piperitenone oxide型。M.arvensis-01群体精油的化学型为limonene-carvone-piperitenone oxide型。M. aquatica群体精油包含4种化学型：①menthone-menthol-pulegone型、②Hnalool-linalyl acetate型、③limenone-carvone型、④1,8-cineole-globulol型。M×piperit群体精油含有2种化学型：high menthone-low menthol型和low menthone-high menthol型。M. longifolia群体精油含有2种化学型：limonene-carvone型和piperitone-isocaryophyllene oxide-piperitenone oxide型。M. suaveolens群体精油化学型为：piperitenone oxide型。M. canadensis群体精油含有两种化学型：menthol-menthyl acetate型和menthone-menthol型。Mxgracilis群体精油的化学型为limonene-carvone型。M. pulegium L群体精油的化学型为menthone-pulegone-menthofuran型。结果表明,所收集的薄荷品种精油存在丰富的化学多样性。精油化学型与cpDNA标记相关性分析表明,薄荷品种精油化学型分析结果与cpDNA标记聚类结果具有紧密的相关性,说明二者之间存在一定的相互关系。
5.薄荷资源AFLP遗传多样性分析。分析结果表明,28份薄荷品种间的遗传相似系数0.44-0.91之间,平均相似系数为0.66。聚类分析结果将28个薄荷品种分为7个主要的聚类,分别为①M.spicata-M.suaveolens,②M×piperit,③M.aquatica-Mxgracilis,④M.spicata-M. longifolia,⑤M.aquatica,⑥M.canadensis-M.arvensis和⑦M.spicata-M.longifolia。结果表明所收集的薄荷品种存在丰富的遗传多样性。
Mint is the most important plant in the genus Mentha (Lamiaceae). All species and natural hybrids of Mentha are essential oil bearing; these essential oils have high economic value and are now widely used in foods, medicines industry and daily chemical field. In this article, we evaluated the leaf characters, biomass, production and composition of essential oil of the mint germplasm resources. The chemical and genetic diversity were also analyzed using chemical (composition of essential oil) and molecular marker (Amplified Fragment Length Polymorphism and chloroplast DNA) respectively.
The main results were described as follows:
1. Evaluation on foliage traits and essential oil production of mint germplasm resources. The results of leaf traits variation showed that great variation was found in leaf traits except the the trait of leaf length/width ratio. The coefficient of variation of these leaf traits was above35%. The leaf weight of M×gracilis-01was highest (0.15g) in the leaf weight trait. The leaf length/width ratio of M. aquatica-01was highest (2.5) in the leaf length/width ratio trait. The leaf area of M. spicata-06was highest (7.8cm2) in the leaf area trait. The oil gland numbers of M.spicata-02were highest (8475) in the oil gland numbers trait. The results of essential oil content, fresh yield and oil production traits showed that great variation was found in these traits except the the trait of fresh yield, the coefficient of variation of these traits was above50%. The fresh yield of M. spicata-08was highest (1467.4kg/mu) in the fresh yield trait. The oil content and production of M. canadensis-02were highest (0.92%and10.3kg respectively) in the content and production of essential oil trait. Correlation analysis showed that the leaf area had very significant positive correlation with content of essential oil, oil production, leaf length/width ratio and oil gland number. The oil production had significant positive correlation with content of essential oil. According to the comprehensive evaluation of oil production, there were ten mint germplasm resources whose per mu production of essential oil in more than6.0kg were selected. Our results showed that there were plentiful variation in leaf and oil production traits and extremely significant correlation among these characters in mint germplasm resources.
2. Study and evaluation on compositions of mint oil. The analysis results of compositions of mint oil showed that in total, twenty-seven main compounds whose contents were above5.0%were identified. The contents of these compounds had difference at different flowering stage, the levels of seven compounds reached the maximum in the initial flowering stage, the levels of sixteen compounds reached the maximum in the full flowering stage, and the levels of four compounds reached the maximum in the later flowering stage. Exogenous abscisic acid (ABA) application caused a decline in oil quality by improving the content of menthofuran and reduceing the content of menthol of mint oil, correlation analysis showed that a dynamic balance among the contents of menthol, menthone, menthofuran and pulegone was found, the relationship was menthol=74.78-1.044×menthone-0.705×menthofuran0.796×pulegone. gene expression analysis showed that ABA application affected the expression of genes in metabolic pathways of mint oil. According to the result of evaluation on essential oil compounds, there were20mint germplasm resources that had the potential to produce high quality oil were selected.
3. Analysis on relationship of mint germplasm resources using chloroplast DNA marker. The results showed that two main clusters (cluster I and cluster II) were found by neighbour-joining method. Cluster I included M. spicata-M. suaveolens-M. longifolia-M.×gracilis-M. arvensis-M×piperita, and Cluster II included M. aquatica-M.×piperita-M.×piperita-01, the Cluster I was divided three subgroups:①M. spicata-M. longifolia、②M. spicata-M. suaveolens and③M.arvensis-M.×gracilis, the Cluster II was divided two subgroup:①M. canadensis-M.aquatica and②M.×piperita. Our results reflected the relationship between parent lines and offspring lines.
4. Analysis on diversity of chemotype of mint germplasm resources. M. spicata accessions included two chemtypes, one was limonene-carvone type, another was1,8-cineole-piperitenone oxide type. The chemotype of M.arvensis-01belonged to limonene-carvone-piperitenone oxide type. The M. aquatica accessions were divided into four chemotype:①menthone-menthol-pulegone type,②linalool-linalyl acetate type,③limenone-carvone type,④1,8-cineole-globulol type. The M×piperit accessions included two chemotypes, one was high menthone-low menthol type, another was low menthone-high menthol type. The M. longifolia accessions were divided into two chemotype, one was limonene-carvone type, and another was piperitone-isocaryophyllene oxide-piperitenone oxide type. The chemotype of M. suaveolens accessions belonged to piperitenone oxide type. The M. canadensis included two chemotypes:menthol-menthyl acetate type and menthone-menthol type. The chemotype of M×gracilis and M. pulegium L. belonged to limonene-carvone type and menthone-pulegone-menthofuran type respectively. The results showed that there were rich chemotypes among these mint germplasm resources that we collected.
Correlation analysis between chemotype and chloroplast DNA marker. There was a consistent relationship between chemotype and chloroplast DNA marker, which hinted that there was a certain correlation between chemotype and parent lines.
6. Analysis on genetic diversity of mint germplasm resources using amplified fragment length polymorphism (AFLP). The similarity coefficient was between0.44and0.91among the mint germplasm resources, and the average similarity coefficient was0.66. The28mint germplasm resources were could be divided into seven groups:①M. spicata-M. suaveolens,②M×piperit,③M. aquatica-M×gracilis,④M.spicata-M. longifolia,⑤M. aquatica,⑥M. canadensis-M.arvensis and⑦M. spicata-M. longifolia. Our results showed that there was rich genomic diversity among these mint germplasm resources.
1.薄荷叶片性状及精油产量评价。对薄荷叶片性状变异分析表明,除叶长宽比的变异系数较低外,其余各性状的变异系数均在35%以上,其中M×gracilis-01的叶片重量最大为0.15g,M. aquatica-01的叶长宽比最大为2.5,M. spicata-06的叶面积最大为7.8cm2,M.spicata-02的油腺数量最多为8475个。对精油含量、鲜草产量和精油产量变异分析表明,除鲜草产量的变异系数较低外,精油含量和精油产量的变异系数均在50%以上,其中M. spicata-08的鲜草亩产量最高为1467.4kg,M.canadensis-02的精油含量和精油亩产量最高分别为0.92%和10.3kg。相关性分析显示叶面积与精油产量、精油含量和叶长宽比之间显著正相关,与油腺数量呈现极显著正相关；精油产量与精油含量呈现极显著正相关。对薄荷精油产量进行综合评价显示精油亩产量在6.0kg以上的薄荷资源有M. canadensis-02等10种。结果表明所收集的薄荷品种在叶片性状和精油产量方面存在丰富的变异,在一些重要性状之间存在极显著的相关性。
2.薄荷精油成分研究及评价。精油成分分析结果表明,薄荷精油成分呈现多样性,其中相对含量在5.0%以上有menthol等27种精油成分。不同花期精油成分分析结果表明,精油成分相对含量在不同花期存在差异性变化,其中globulol等7种精油成分相对含量在初花期最高。menthol等16种精油成分相对含量在盛花期最高。limonene等4种精油成分相对含量在末花期最高。脱落酸处理对薄荷精油成分影响的分析结果表明,经脱落酸处理后menthofuran含量显著提高导致精油品质下降。脱落酸处理后的薄荷精油主成分相对含量间的相关性分析表明,薄荷精油各主成分相对含量间存在动态平衡,变化规律符合线性方程menthol=74.787-1.044×menthone-0.705×menthofuran-0.796×pulegonec脱落酸处理后的薄荷精油合成相关基因表达分析结果表明,ABA处理会显著影响薄荷精油合成相关基因的表达。精油成分在不同薄荷资源间存在差异性,这些差异是影响精油品质的主要因素,依据精油成分评价,具有生产高品质薄荷精油潜力的种质资源有M. spicata-08等10种。
3.薄荷资源chloroplast DNA亲缘关系分析。所收集的薄荷品种资源共产生了两个主要的聚类,聚类I包括M. spicata-M. suaveolens-M. longifolia-M.×gracilis-M. arvensis-M×piperita等薄荷群体,聚类II包括M. aquatica-M. xpiperita-M.×piperita等薄荷群体。其中聚类1分为3个亚组:①M. spicata-M. longifolia、②M. spicata-M. suaveolens和③M.arvensis-M. xgracilis,聚类11分为2个亚组：①M. canadensis-M.aquatica和②M.×piperita。结果表明,亲缘关系相近的薄荷群体聚为同一类,同时本研究结果清晰的反映了母本与其后代之间的亲缘关系。
4.薄荷资源精油化学型分析。M. spicata群体精油包含2个化学型：limonene-carvone型和1,8-cineole-piperitenone oxide型。M.arvensis-01群体精油的化学型为limonene-carvone-piperitenone oxide型。M. aquatica群体精油包含4种化学型：①menthone-menthol-pulegone型、②Hnalool-linalyl acetate型、③limenone-carvone型、④1,8-cineole-globulol型。M×piperit群体精油含有2种化学型：high menthone-low menthol型和low menthone-high menthol型。M. longifolia群体精油含有2种化学型：limonene-carvone型和piperitone-isocaryophyllene oxide-piperitenone oxide型。M. suaveolens群体精油化学型为：piperitenone oxide型。M. canadensis群体精油含有两种化学型：menthol-menthyl acetate型和menthone-menthol型。Mxgracilis群体精油的化学型为limonene-carvone型。M. pulegium L群体精油的化学型为menthone-pulegone-menthofuran型。结果表明,所收集的薄荷品种精油存在丰富的化学多样性。精油化学型与cpDNA标记相关性分析表明,薄荷品种精油化学型分析结果与cpDNA标记聚类结果具有紧密的相关性,说明二者之间存在一定的相互关系。
5.薄荷资源AFLP遗传多样性分析。分析结果表明,28份薄荷品种间的遗传相似系数0.44-0.91之间,平均相似系数为0.66。聚类分析结果将28个薄荷品种分为7个主要的聚类,分别为①M.spicata-M.suaveolens,②M×piperit,③M.aquatica-Mxgracilis,④M.spicata-M. longifolia,⑤M.aquatica,⑥M.canadensis-M.arvensis和⑦M.spicata-M.longifolia。结果表明所收集的薄荷品种存在丰富的遗传多样性。
Mint is the most important plant in the genus Mentha (Lamiaceae). All species and natural hybrids of Mentha are essential oil bearing; these essential oils have high economic value and are now widely used in foods, medicines industry and daily chemical field. In this article, we evaluated the leaf characters, biomass, production and composition of essential oil of the mint germplasm resources. The chemical and genetic diversity were also analyzed using chemical (composition of essential oil) and molecular marker (Amplified Fragment Length Polymorphism and chloroplast DNA) respectively.
The main results were described as follows:
1. Evaluation on foliage traits and essential oil production of mint germplasm resources. The results of leaf traits variation showed that great variation was found in leaf traits except the the trait of leaf length/width ratio. The coefficient of variation of these leaf traits was above35%. The leaf weight of M×gracilis-01was highest (0.15g) in the leaf weight trait. The leaf length/width ratio of M. aquatica-01was highest (2.5) in the leaf length/width ratio trait. The leaf area of M. spicata-06was highest (7.8cm2) in the leaf area trait. The oil gland numbers of M.spicata-02were highest (8475) in the oil gland numbers trait. The results of essential oil content, fresh yield and oil production traits showed that great variation was found in these traits except the the trait of fresh yield, the coefficient of variation of these traits was above50%. The fresh yield of M. spicata-08was highest (1467.4kg/mu) in the fresh yield trait. The oil content and production of M. canadensis-02were highest (0.92%and10.3kg respectively) in the content and production of essential oil trait. Correlation analysis showed that the leaf area had very significant positive correlation with content of essential oil, oil production, leaf length/width ratio and oil gland number. The oil production had significant positive correlation with content of essential oil. According to the comprehensive evaluation of oil production, there were ten mint germplasm resources whose per mu production of essential oil in more than6.0kg were selected. Our results showed that there were plentiful variation in leaf and oil production traits and extremely significant correlation among these characters in mint germplasm resources.
2. Study and evaluation on compositions of mint oil. The analysis results of compositions of mint oil showed that in total, twenty-seven main compounds whose contents were above5.0%were identified. The contents of these compounds had difference at different flowering stage, the levels of seven compounds reached the maximum in the initial flowering stage, the levels of sixteen compounds reached the maximum in the full flowering stage, and the levels of four compounds reached the maximum in the later flowering stage. Exogenous abscisic acid (ABA) application caused a decline in oil quality by improving the content of menthofuran and reduceing the content of menthol of mint oil, correlation analysis showed that a dynamic balance among the contents of menthol, menthone, menthofuran and pulegone was found, the relationship was menthol=74.78-1.044×menthone-0.705×menthofuran0.796×pulegone. gene expression analysis showed that ABA application affected the expression of genes in metabolic pathways of mint oil. According to the result of evaluation on essential oil compounds, there were20mint germplasm resources that had the potential to produce high quality oil were selected.
3. Analysis on relationship of mint germplasm resources using chloroplast DNA marker. The results showed that two main clusters (cluster I and cluster II) were found by neighbour-joining method. Cluster I included M. spicata-M. suaveolens-M. longifolia-M.×gracilis-M. arvensis-M×piperita, and Cluster II included M. aquatica-M.×piperita-M.×piperita-01, the Cluster I was divided three subgroups:①M. spicata-M. longifolia、②M. spicata-M. suaveolens and③M.arvensis-M.×gracilis, the Cluster II was divided two subgroup:①M. canadensis-M.aquatica and②M.×piperita. Our results reflected the relationship between parent lines and offspring lines.
4. Analysis on diversity of chemotype of mint germplasm resources. M. spicata accessions included two chemtypes, one was limonene-carvone type, another was1,8-cineole-piperitenone oxide type. The chemotype of M.arvensis-01belonged to limonene-carvone-piperitenone oxide type. The M. aquatica accessions were divided into four chemotype:①menthone-menthol-pulegone type,②linalool-linalyl acetate type,③limenone-carvone type,④1,8-cineole-globulol type. The M×piperit accessions included two chemotypes, one was high menthone-low menthol type, another was low menthone-high menthol type. The M. longifolia accessions were divided into two chemotype, one was limonene-carvone type, and another was piperitone-isocaryophyllene oxide-piperitenone oxide type. The chemotype of M. suaveolens accessions belonged to piperitenone oxide type. The M. canadensis included two chemotypes:menthol-menthyl acetate type and menthone-menthol type. The chemotype of M×gracilis and M. pulegium L. belonged to limonene-carvone type and menthone-pulegone-menthofuran type respectively. The results showed that there were rich chemotypes among these mint germplasm resources that we collected.
Correlation analysis between chemotype and chloroplast DNA marker. There was a consistent relationship between chemotype and chloroplast DNA marker, which hinted that there was a certain correlation between chemotype and parent lines.
6. Analysis on genetic diversity of mint germplasm resources using amplified fragment length polymorphism (AFLP). The similarity coefficient was between0.44and0.91among the mint germplasm resources, and the average similarity coefficient was0.66. The28mint germplasm resources were could be divided into seven groups:①M. spicata-M. suaveolens,②M×piperit,③M. aquatica-M×gracilis,④M.spicata-M. longifolia,⑤M. aquatica,⑥M. canadensis-M.arvensis and⑦M. spicata-M. longifolia. Our results showed that there was rich genomic diversity among these mint germplasm resources.
引文
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[10]中国对印度薄荷脑的需求.国内外香化信息,2008(6)：6.
[11]国内薄荷油9月上旬走势.国内外香化信息,2010(9)：3-4.
[12]国内薄荷油近期行情报告.国内外香化信息,2011(3)：6.
[13]国内薄荷油近期行情报告.国内外香化信息,2011(4)：6.
[14]国内薄荷油市场现状分析-难过的一道坎.国内外香化信息,2006(7)：4-5.
[15]国内各地几种产品的行情报价.国内外香化信息,2011(9)：5.
[16]近期国内薄荷市场报告.国内外香化信息,2010(8)：5.
[17]近期国内薄荷油市场报告.国内外香化信息,2010(11)：3.
[18]今日薄荷市场明显回落.国内外香化信息,2004(10)：7.
[19]太和薄荷复兴之路受挫.国内外香化信息,2012(1)：5.
[20]太和基地考察报告.国内外香化信息,2006(9)：5-6.
[21]我国薄荷脑隐退国际市场个中原因要深思.国内外香化信息,2004(10)：13.
[22]我国薄荷育苗其的突破.国内外香化信息,2003(10)：11.
[23]Valtcho, Zheljazkov D. Peppermint productivity and oil composition as a function of nitrogen, growth stage, and harvest time. Agron J,2010(102):24-128.
[24]Kumar S, Suresh R, Singh V, Singh AK. Economic analysis of menthol mint cultivation in Uttar Pradesh:a case study of Barabanki district. Agricultural Economics Research Review,2011(24):345-350.
[25]1月11日印度薄荷类产品价格及行情月评.国内外香化信息,2012(1)：10.
[26]2-3月份印度薄荷类产品行情.国内外香化信息,2008(3)：12.
[27]3月份印度薄荷类产品现货／期货行情.国内外香化信息,2007(3)：10.
[28]4月薄荷脑国际市场评估.国内外香化信息,2009(4)：5-6.
[29]4月薄荷脑国际市场评估.国内外香化信息,2009(5)：3.
[30]4月上旬印度薄荷油综合分析以及国内市场走向.国内外香化信息,2007(4)：5.
[31]6月份印度薄荷类产品行情.国内外香化信息,2007(6)：9.
[32]7-8月份印度薄荷类产品行情.国内外香化信息,2008(8)：10.
[33]8-9月份印度薄荷类产品行情.国内外香化信息,2008(9)：12.
[34]8-9月份印度薄荷类产品行情.国内外香化信息,2008(9)：8.
[35]9月15日印度薄荷类产品现货价格及行情月评.国内外香化信息,2011(9)：10.
[36]10～11月份印度薄荷类产品行情.国内外香化信息,2008(11)：10.
[37]10月15日印度薄荷类产品现货价格及行情月评.国内外香化信息,2011(10)：8.
[38]11月19日印度薄荷类产品现货价格及行情月评.国内外香化信息,2011(11)：4.
[39]11月份印度薄荷类产品行情.国内外香化信息,2007(11)：11.
[40]12-1月份印度薄荷类产品行情.国内外香化信息,2008(1)：11.
[41]2007年1月我国精油进出口情况.国内外香化信息,2007(3)：7.
[42]2007年2月我国精油进出口情况.国内外香化信息,2007(4)：9.
[43]2007年3月我国精油进出口情况.国内外香化信息,2007(5)：8.
[44]2007年4月我国精油进出口情况.国内外香化信息,2007(6)：6.
[45]2007年上半年国内部分香料市场动态.国内外香化信息,2007(6)：2.
[46]2007年6月我国精油进出口情况.国内外香化信息,2007(8)：9.
[47]2007年7月我国精油进出口情况.国内外香化信息,2007(9)：9.
[48]2007年8月我国精油进出口情况.国内外香化信息,2007(10)：9.
[49]2007年9月我国精油进出口情况.国内外香化信息,2007(11)：8.
[50]2007年10月我国精油进出口情况.国内外香化信息,2007(12)：7.
[51]2007年11月我国精油进出口情况.国内外香化信息,2008(1)：8.
[52]2008年1月我国精油进出口情况.国内外香化信息,2008(3)：9-10.
[53]2008年2月我国精油进出口情况.国内外香化信息,2008(4)：7.
[54]2008年3月我国精油进出口情况.国内外香化信息,2008(5)：7-8.
[55]2008年4月我国精油进出口情况.国内外香化信息,2008(6)：9-10.
[56]2008年5月我国精油进出口情况.国内外香化信息,2008(7)：9-10.
[57]2008年6月我国精油进出口情况.国内外香化信息,2008(8)：6-7.
[58]2008年7月我国精油进出口情况.国内外香化信息,2008(9)：5-6.
[59]2008年8月我国精油进出口情况.国内外香化信息,2008(10)：5-6.
[60]2008年9月我国精油进出口情况.国内外香化信息,2008(11)：6-7.
[61]2008年10月我国精油进出口情况.国内外香化信息,2008(12)：7-8.
[62]2008年11月我国精油进出口情况.国内外香化信息,2009(1)：8-9.
[63]2009年1月我国精油进出口情况.国内外香化信息,2009(3)：8-9.
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[2]芳香原料特写：薄荷脑的历史、消费量、合成、副产品、替代品和衍生物(一).国内外香化信息,2009(4)：6.
[3]芳香原料特写：薄荷脑的历史、消费量、合成、副产品、替代品和衍生物(二).国内外香化信息,2009(5)：6-7.
[7]芳香原料特写：薄荷脑的历史、消费量、合成、副产品、替代品和衍生物(三).国内外香化信息,2009(6)：5-6.
[8]芳香原料特写：薄荷脑的历史、消费量、合成、副产品、替代品和衍生物(三).国内外香化信息,2009(7)：5-6.
[9]芳香原料特写：薄荷脑的历史、消费量、合成、副产品、替代品和衍生物(三).国内外香化信息,2009(8)：3-4.
[10]中国对印度薄荷脑的需求.国内外香化信息,2008(6)：6.
[11]国内薄荷油9月上旬走势.国内外香化信息,2010(9)：3-4.
[12]国内薄荷油近期行情报告.国内外香化信息,2011(3)：6.
[13]国内薄荷油近期行情报告.国内外香化信息,2011(4)：6.
[14]国内薄荷油市场现状分析-难过的一道坎.国内外香化信息,2006(7)：4-5.
[15]国内各地几种产品的行情报价.国内外香化信息,2011(9)：5.
[16]近期国内薄荷市场报告.国内外香化信息,2010(8)：5.
[17]近期国内薄荷油市场报告.国内外香化信息,2010(11)：3.
[18]今日薄荷市场明显回落.国内外香化信息,2004(10)：7.
[19]太和薄荷复兴之路受挫.国内外香化信息,2012(1)：5.
[20]太和基地考察报告.国内外香化信息,2006(9)：5-6.
[21]我国薄荷脑隐退国际市场个中原因要深思.国内外香化信息,2004(10)：13.
[22]我国薄荷育苗其的突破.国内外香化信息,2003(10)：11.
[23]Valtcho, Zheljazkov D. Peppermint productivity and oil composition as a function of nitrogen, growth stage, and harvest time. Agron J,2010(102):24-128.
[24]Kumar S, Suresh R, Singh V, Singh AK. Economic analysis of menthol mint cultivation in Uttar Pradesh:a case study of Barabanki district. Agricultural Economics Research Review,2011(24):345-350.
[25]1月11日印度薄荷类产品价格及行情月评.国内外香化信息,2012(1)：10.
[26]2-3月份印度薄荷类产品行情.国内外香化信息,2008(3)：12.
[27]3月份印度薄荷类产品现货／期货行情.国内外香化信息,2007(3)：10.
[28]4月薄荷脑国际市场评估.国内外香化信息,2009(4)：5-6.
[29]4月薄荷脑国际市场评估.国内外香化信息,2009(5)：3.
[30]4月上旬印度薄荷油综合分析以及国内市场走向.国内外香化信息,2007(4)：5.
[31]6月份印度薄荷类产品行情.国内外香化信息,2007(6)：9.
[32]7-8月份印度薄荷类产品行情.国内外香化信息,2008(8)：10.
[33]8-9月份印度薄荷类产品行情.国内外香化信息,2008(9)：12.
[34]8-9月份印度薄荷类产品行情.国内外香化信息,2008(9)：8.
[35]9月15日印度薄荷类产品现货价格及行情月评.国内外香化信息,2011(9)：10.
[36]10～11月份印度薄荷类产品行情.国内外香化信息,2008(11)：10.
[37]10月15日印度薄荷类产品现货价格及行情月评.国内外香化信息,2011(10)：8.
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