白菜型冬油菜抗寒性的遗传及SSR标记与蛋白质表达分析
|
摘要
白菜型冬油菜(Brassica.rapa, L.,var.biennis,f.winterness)具有优异的抗寒性,既是重要的油料作物,又是冬、春季覆盖作物,在北方地区的农业生产中具有重大的经济意义和生态意义。
本研究利用白菜型冬油菜超强抗寒性品种陇油6号和抗寒性较弱品种天油8号进行正反交得到F_1代以及F_2代。以F_2代两群体为基础研究群体,对白菜型冬油菜的抗寒性的遗传、抗寒性相关的SSR分子标记以及不同抗寒品种的蛋白质表达等进行了分析研究,主要研究结果如下:
1.通过陇油6号和天油8号F_2代植株的抗寒与不抗寒分离比例进行统计,实测值与理论值的比率用卡方适合性进行测验,确定分离比率。结果表明,白菜型冬油菜抗寒性是受两对主基因所控制的,用R1和R2表示,这两对基因作用于同一性状,其效应是累加的,等位基因间无显隐性关系;用抗寒性弱的亲本做母本,分析比例有一定偏离。正反交分离比不同,说明白菜型油菜的抗寒性遗传除受细胞核基因控制外,还与细胞质遗传有关。
2.利用SSR分子标记引物对陇油6号和天油8号的508个F_2代植株分析结果,获得了7个与白菜型冬油菜抗寒性Rf紧密连锁的分子标记,应用Mapdraw软件构建了遗传图谱。7个标记与抗寒连锁程度由近及远分别是:BRMS-050、Ol12-A04、BRMS-042-2、BRMS-036、Ra3-D02b、Na10-C06、Ra3-D04,连锁距离分别为:4.09 cM、5.83 cM、9.49 cM、12.73 cM、12.96 cM、16.88 cM、24.61 cM,其中,标记BRMS-050、Ol12-A04距抗寒性Rf的遗传距离较近,这些标记可以作为白菜型冬油菜分子标记辅助选择育种的参考依据。
3.对冬油菜亲本材料陇油6号、天油8号进行蛋白质双向电泳(2-DE)分析,在1000μg/IPG胶条的上样量条件下,超强抗寒性品种陇油6号和弱抗寒性品种天油8号分别检测到有效蛋白点1207和1053个。以天油8号蛋白质胶为参照胶,陇油6号与之比较,差异蛋白点有76个,其中49个蛋白点表达量上调,21个蛋白点表达量下调,新增的蛋白点5个,减少的蛋白点1个。结果表明,白菜型冬油菜陇油6号的抗寒性与某些蛋白质的表达量有关,抗寒性强的品种蛋白质表达量高于抗寒性弱得品种,陇油6号的强抗寒性还与特异蛋白的表达有关。
Winter rapeseed (Brassica campestris) has excellent cold hardiness, it is not only major oil crop, but it is winter and spring cover crop, and it causes great economic and ecological significance on agricultural production in the north areas.
This study used two varieties of winter Brassica.Rapeseeds: super-cold Longyou6 and weaker-cold Tianyou8, obtained F_1 and F_2 generation through reciprocal cross. The two groups of F_2 generation were used for Basic research. Analyzed and research on genetic of cold hardiness in winter Brassica Rapa, SSR marker be relared to cold hardiness and protein expression of different cold hardiness varieties. The main results are as follows:
1. Statistical separation ratio about the number of F_2 generation plants of Longyou6 and Tianyou8 by cold hardiness or not. The ratio of measurement values and the theoretical value used chi-square Suitability for testing, to checking separation ratio. The results showed that cold resistance of winter Brassica rapeseed was controlled by two the main genes, which expressed with R1 and R2. The two pairs of genes acted on the same characters, the effect was cumulative, and there was no recessive relationship between the allele. Separation ratio has deviation when weaker-cold varieties as female parent.Reciprocal cross ratio was different, it declared that the cold resistance heredity of winter Brassica rapeseed not only controlled by nuclear genes, but it connected with the cytoplasmic heredity.
2. Using the primer of SSR molecular marker analysis of the 508 per plant of F_2 generation of Longyou6 and Tianyou8, and the results following: which totally got 7 molecular markers closely linked with cold hardiness Rf in winter Brassica rapa. Applying software of Mapdraw builds genetic map. 7 markers and linkage degree of cold hardiness from near to far were as follows: BRMS-050、Ol12-A04、BRMS-042-2、BRMS-036、Ra3-D02b、Na10-C06、Ra3-D04, Linkage distance was that: 4.09、5.83、9.49、12.73、12.96、16.88、24.61, Among them, the marks BRMS-050, Ol12-A04 was nearer to the cold hardiness gene Rf genes from the genetic distance, These marks can be used as reference of molecule marker assisted selection breeding in winter Brassica rapa.
3. Analyzed on parental materials Longyou6 and Tianyou8 of winter rapeseed with two-dimensional electrophoresis of proteins (2-DE). When loading quantities were at 1000μg/IPG strips. Super cold varieties Longyou6 and weaker-cold varieties Tianyou8 were detected effective protein spots 1207 and 1053. Using Tianyou8 protein gel as the reference gel, Longyou6 in contrast, there were 76 differential protein spots, and in them, the expression of 49 protein level spots increased, 21 protein points decreased, increased 5 new protein spots and reduced 1 protein spots. The results showed that cold resistance of winter Brassica rapa Longyou6 related to the expression level of certain proteins, the varieties of strong cold hardiness had a high expression level of protein than weaker-cold varieties. Strong cold hardiness of Longyou6 also related to the specific protein expression.
本研究利用白菜型冬油菜超强抗寒性品种陇油6号和抗寒性较弱品种天油8号进行正反交得到F_1代以及F_2代。以F_2代两群体为基础研究群体,对白菜型冬油菜的抗寒性的遗传、抗寒性相关的SSR分子标记以及不同抗寒品种的蛋白质表达等进行了分析研究,主要研究结果如下:
1.通过陇油6号和天油8号F_2代植株的抗寒与不抗寒分离比例进行统计,实测值与理论值的比率用卡方适合性进行测验,确定分离比率。结果表明,白菜型冬油菜抗寒性是受两对主基因所控制的,用R1和R2表示,这两对基因作用于同一性状,其效应是累加的,等位基因间无显隐性关系;用抗寒性弱的亲本做母本,分析比例有一定偏离。正反交分离比不同,说明白菜型油菜的抗寒性遗传除受细胞核基因控制外,还与细胞质遗传有关。
2.利用SSR分子标记引物对陇油6号和天油8号的508个F_2代植株分析结果,获得了7个与白菜型冬油菜抗寒性Rf紧密连锁的分子标记,应用Mapdraw软件构建了遗传图谱。7个标记与抗寒连锁程度由近及远分别是:BRMS-050、Ol12-A04、BRMS-042-2、BRMS-036、Ra3-D02b、Na10-C06、Ra3-D04,连锁距离分别为:4.09 cM、5.83 cM、9.49 cM、12.73 cM、12.96 cM、16.88 cM、24.61 cM,其中,标记BRMS-050、Ol12-A04距抗寒性Rf的遗传距离较近,这些标记可以作为白菜型冬油菜分子标记辅助选择育种的参考依据。
3.对冬油菜亲本材料陇油6号、天油8号进行蛋白质双向电泳(2-DE)分析,在1000μg/IPG胶条的上样量条件下,超强抗寒性品种陇油6号和弱抗寒性品种天油8号分别检测到有效蛋白点1207和1053个。以天油8号蛋白质胶为参照胶,陇油6号与之比较,差异蛋白点有76个,其中49个蛋白点表达量上调,21个蛋白点表达量下调,新增的蛋白点5个,减少的蛋白点1个。结果表明,白菜型冬油菜陇油6号的抗寒性与某些蛋白质的表达量有关,抗寒性强的品种蛋白质表达量高于抗寒性弱得品种,陇油6号的强抗寒性还与特异蛋白的表达有关。
Winter rapeseed (Brassica campestris) has excellent cold hardiness, it is not only major oil crop, but it is winter and spring cover crop, and it causes great economic and ecological significance on agricultural production in the north areas.
This study used two varieties of winter Brassica.Rapeseeds: super-cold Longyou6 and weaker-cold Tianyou8, obtained F_1 and F_2 generation through reciprocal cross. The two groups of F_2 generation were used for Basic research. Analyzed and research on genetic of cold hardiness in winter Brassica Rapa, SSR marker be relared to cold hardiness and protein expression of different cold hardiness varieties. The main results are as follows:
1. Statistical separation ratio about the number of F_2 generation plants of Longyou6 and Tianyou8 by cold hardiness or not. The ratio of measurement values and the theoretical value used chi-square Suitability for testing, to checking separation ratio. The results showed that cold resistance of winter Brassica rapeseed was controlled by two the main genes, which expressed with R1 and R2. The two pairs of genes acted on the same characters, the effect was cumulative, and there was no recessive relationship between the allele. Separation ratio has deviation when weaker-cold varieties as female parent.Reciprocal cross ratio was different, it declared that the cold resistance heredity of winter Brassica rapeseed not only controlled by nuclear genes, but it connected with the cytoplasmic heredity.
2. Using the primer of SSR molecular marker analysis of the 508 per plant of F_2 generation of Longyou6 and Tianyou8, and the results following: which totally got 7 molecular markers closely linked with cold hardiness Rf in winter Brassica rapa. Applying software of Mapdraw builds genetic map. 7 markers and linkage degree of cold hardiness from near to far were as follows: BRMS-050、Ol12-A04、BRMS-042-2、BRMS-036、Ra3-D02b、Na10-C06、Ra3-D04, Linkage distance was that: 4.09、5.83、9.49、12.73、12.96、16.88、24.61, Among them, the marks BRMS-050, Ol12-A04 was nearer to the cold hardiness gene Rf genes from the genetic distance, These marks can be used as reference of molecule marker assisted selection breeding in winter Brassica rapa.
3. Analyzed on parental materials Longyou6 and Tianyou8 of winter rapeseed with two-dimensional electrophoresis of proteins (2-DE). When loading quantities were at 1000μg/IPG strips. Super cold varieties Longyou6 and weaker-cold varieties Tianyou8 were detected effective protein spots 1207 and 1053. Using Tianyou8 protein gel as the reference gel, Longyou6 in contrast, there were 76 differential protein spots, and in them, the expression of 49 protein level spots increased, 21 protein points decreased, increased 5 new protein spots and reduced 1 protein spots. The results showed that cold resistance of winter Brassica rapa Longyou6 related to the expression level of certain proteins, the varieties of strong cold hardiness had a high expression level of protein than weaker-cold varieties. Strong cold hardiness of Longyou6 also related to the specific protein expression.
引文
[1]刘后利.油菜的遗传和育种[M].上海:上海科技出版社,1985.
[2]孙万仓,马卫国,雷建民,等.冬油菜在西北旱寒区的适应性和北移的可行性研究[J].中国农业科学,2007, 40(12):2716-2726.
[3]朱惠霞,孙万仓,邓斌,等.白菜型冬油菜品种的抗寒性及其生理生化特性[J].西北农业学报,2007,16(4): 34-38.
[4]蒲媛媛,孙万仓.白菜型冬油菜抗寒性与生理生化特性关系[J].分子植物育种,2010,8(2),335-339.
[5]盖玥,牛俊义,孙万仓,等.降温处理对白菜型油菜品种抗寒生理指标的影响[J].甘肃农业大学学报,2005, 40(2)182-185.
[6]宋良图,郭书普.醋酶同工酶和过氧化物同工酶与油菜抗寒性的关系[J].安徽农业科学,1995,23(4): 333-335.
[7]苏维埃.植物对温度逆境的适应[A].植物生理与分子生物学[M].北京:科学出版社,2003:721-738.
[8]周瑞莲,赵哈林.高寒山区牧草根质膜和脂肪酸组分对冷冻低温的适应反应[J].植物生理学报,2001, 25(1):115-118.
[9]Deng L Y. Study on relationship between membrane lips fatty acid and winter hardy in grape[J]. Acta Phytophysiologia Sinica, 1982(8): 273-281.
[10]Murelli C F, Rizza G, Marinone F L. Metabolic changes associated with cold acclimation in contrasting cultivars of barley[J]. Physiologia plantarum, 1995, 94: 87-93.
[11]江勇,贾士荣,费云标.抗冻蛋白及其在植物抗冻生理中的作用[J].植物学报,1999,41(7):677-685.
[12]Fei Y B, Shu N H, Huang T, et al. Cellular and biochemical cor-relates to chilling and freezing tolerance in plant[J]. Prog Biotech, 1994, 14(3): 40-43.
[13]周丽英,杨丽涛,郑坚瑜.植物抗寒冻基因工程研究进展[J].植物学通报,2001,18(3):325-331.
[14]陈亚华,沈振国,刘友良.低温、高pH胁迫对水稻根系质膜、液泡膜ATP酶活性的影响[J].植物生理学报,2000,26(5):407-412.
[15]杨德浩,杨敏生,王进茂,等.欧洲白桦苗期低温胁迫时膜系统的变化[J].东北林业大学学报,2004,32(6): 13-15.
[16]艾琳,张萍,胡成志.低温胁迫对葡萄根系膜系统和可溶性糖及脯氨酸含量的影响[J].新疆农业大学学报,2004,27(4):47-50.
[17]P. H. LI. Plant Cold Tolerance and Freezing Stress[J]. New York, Academic Press, 1987: 37-48.
[18]Lyons, J. M. Chilling injury in plant[J]. Ann Rev Plant physiol, 1975, 24: 445-466.
[19]张泽煌,黄碧琦,陈钟佃,等.低温胁迫对茄子的伤害及茄子抗寒机理[J].福建农业学报,2000,15(1):40- 42.
[20]刘鸿先,曾韶西,王以柔,等.低温对不同耐寒力黄瓜幼苗子叶各细胞器官中SOD的影响[J].植物生理学报,1985,11(1):48-57.
[21]何若韫.植物低温逆境生理[M].北京:中国农业出版社,1995.
[22]马德华,卢育华,庞金安.低温对黄瓜幼苗膜质过氧化的影响[J].园艺学报,1998,25(1):61-64.
[23]张静.番茄苗期低温下生理生化特性的研究[D].硕士学位论文,扬州,扬州大学,2002.
[24]Chen H. H, Li D. H. Plant cold hardness and freezing stress[M]. Academic press, 1982, 5.
[25]谢吉容,向邓云,梅虎,等.南方红豆杉抗寒性的变化与内源激素的关系[J].西南师范大学学报(自然科学版), 2002, 27(2):231-234.
[26]Chen THH, Gusta LV. Abscisic acid-induced freezing re-sistance in cultured plant cells[J]. Plant Physiol, 1983, 73: 71-74.
[27]Violalang, E. T. Palva. Seasonal changes in tissue elasticity in chaparralshrubs[J]. Plant Physiol, 1994, 104(4): 1341-1349.
[28]梁建生.ABA对高等植物基因表达的调节作用[J].植物生理学通讯,1991,27(3):230-233.
[29]于贤昌,邢禹贤,马红,等.李滨低温胁迫下黄瓜嫁接苗和自根苗内源激素的变化[J].园艺学报,1999,26 (6):406-407.
[30]张荣华,李拥军,张叶玲.脯氨酸含量对苜蓿抗寒性影响的研究[J].现代化农业,2006,4:17-22.
[31]赵剑颖,宋晓莉,杨蕊,等.4℃胁迫过程中大叶黄杨和北海道黄杨叶片抗寒生理生化指标的变化[J].北京农学院学报,2010,25(2):57-61.
[32]王瑾,廖康,王燕凌,等.秋季自然降温对野生樱桃李叶片渗透调节物质的影响[J].新疆农业科学,2010, 47(5):952-957.
[33]任丽,岳桦. 4种中原牡丹在哈尔滨地区的抗寒性研究[J].植物研究,2010,30(2): 228-237.
[34]崔国文.紫花苜蓿田间越冬期抗寒生理研究[J].草地学报,2009,17(2):145-150.
[35]亓白岩,周冬琴,於朝广,等.8种含笑属植物的抗寒性研究[J].江苏农业科学,2010(5): 258-263.
[36]简令成,吴素萱.植物抗寒性的细胞学研究-小麦越冬过程中细胞内物质的变化[J].植物学报,1995(13): 1-16.
[37]郝丽娟,邵青玲.不同杏品种的抗寒性研究[J].山西果树,2006(5):3-5.
[38]马艳青,戴雄泽.低温胁迫对辣椒抗寒性相关生理指标的影响[J].湖南农业大学学报:自然科学版, 2000,26(6):461-462.
[39]郑荣梁.自由基生物学[M].北京:高等教育出版社,1992.
[40]王佩芝,符献琼,杜景智.冬小麦抗寒指标的研究[J].新疆农业科学,1997,4:18-22.
[41]孙学成,胡承孝,魏文学.钼肥对冬小麦脯氨酸及抗坏血酸含量的影响[J].三峡大学学报(自然科学版) 2002,23(5):474-477.
[42]李丽,黄先群, Chokchai Wanapu,等.贵州省2008年油菜区试材料的SSR遗传多样性分析[J].贵州农业科学,2010,38(11):1-4.
[43]唐容,王通强,黄泽素,等.利用SSR对甘蓝型黄籽油菜遗传多样性的研究[J].贵州农业科学,2009, 37(2): 7-10.
[44]伍宁丰,李汝刚,伍晓明,等.中国甘蓝型油菜遗传多样性的RAPD分子标记[J].生物多样性,1997,5(4):246-250.
[45]安贤惠,陈宝元,傅廷栋,等.利用RAPD标记研究中国芥菜型油菜遗传多样性[J].华中农业大学学报, 1999,18(6):524-527.
[46]李海渤,杨光圣,傅廷栋,等.部分甘蓝型油菜双低品种(系)的遗传多样性评估[J].韶关学院学报(自然科学版),2003,24(6):96-100.
[47]Diers B W, Osborn T C. Genetic diversity of oilseedBrassica napus germplasmbased on restriction fragment length polymorphisms[J].Theor Appl Genet, 1994, 88(6): 662-668.
[48]马朝芝,Sakai Takako,傅廷栋,等.RAPDs和RFLPs分析甘蓝型杂交油菜亲本的遗传多样性[J].作物学报,2003,29(5):701-707.
[49]曹维荣,王超.分子标记在甘蓝遗传育种中的应用[J].分子植物育种,2003, 4(1):547-550.
[50]Landry B. S, Hubert N, Etoh T, Harada J. J, and Lincoln S. E. A genetic map for Brassica napus based on restriction fragment length polymorphism detected with expressed DNA sequences[J]. Genome, 1991, 34 (4): 543-552.
[51]许鲲,张冬晓,伍晓明,等.国家冬油菜区试新品种SSR指纹图谱构建与遗传关系分析[J].中国油料作物学报,2008,30(1):29-34.
[52]雷天刚,张学昆,陆合,等.甘蓝型油菜黄籽品种SSR指纹图谱的构建[J].西南农业大学学报,2005,27(3): 361-364.
[53]刘平武,周国岭,杨光圣,等.双低甘蓝型油菜杂交种亲本指纹图谱构建和杂交种纯度鉴定[J].作物学报,2005,3l(5):640-646.
[54]唐容,王通强,黄泽素,等.利用SSR对甘蓝型黄籽油菜遗传多样性的研究[J].贵州农业科学,2009,37(2): 7-10.
[55]周红伟,姚艳梅,付忠,等.春性双低甘蓝型油菜杂交种和亲本指纹图谱构建及杂交种纯度鉴定[J].西北农业学报,2010,19(3):81-86.
[56]段院生,刘平武,杨光圣.甘蓝型油菜杂交种亲本指纹图谱分析[J].中国油料作物学报,2002,24(2):5-9.
[57]文雁成,王汉中,沈金雄,等.SRAP和SSR标记构建的甘蓝型油菜品种指纹图谱比较[J].中国油料作物学报,2006,28(3):233-239.
[58]林道哲,董顺文,刘朝辉,等.26份甘蓝型油菜自交系指纹图谱分析[J].安徽农业科学,2008,36(20): 8493-8496.
[59]陈碧云,伍晓明,张冬晓,等.国家冬油菜区试新品种的SSR指纹图谱分析[J].分子植物育种,2008,6(4): 709-716.
[60]钟军,李栒,官春云,等.中国芸芥栽培品种亲缘关系的RAPD分析[J].中国农业科学2004,37(10): 1434-1438.
[61]杨畅,郑普英,张学昆,等.甘蓝型油菜EST分子标记技术体系的建立[J].中国油料作物学报, 2008,30(1): 17-24.
[62]Hu J,. Quiros C, F. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 1991, 10: 505-511.
[63]刘杰,刘公社,李殿荣,等. RAPD方法鉴定油菜“杂油59”种子纯度的研究[J].应用与环境生物学报, 2000, 6(6):526-529.
[64]段院生,刘平武,杨光圣.用RAPD标记鉴定甘蓝型油菜杂种H9909的纯度[J].华中农业大学学报, 2002,21(4):301-305.
[65]汤天泽,肖小余,税红霞.SSR标记与形态学方法鉴定杂交油菜纯度的比较研究[J].分子植物育种, 2008,6(2):377-380.
[66]Burns M J, Barnes S R, Bowman J G, Clarke M H E, Werner C P, Kearsey M J. QTL analysis of an intervarietal set of substitution lines in Brassica napus:seed oil content and fatty acid composition[J]. Heredity, 2003, 90: 39-48.
[67]Mahmood T, Rahman M H, Stringam G R, Yeh F, Good A G. Identification of quantitative trait loci(QTL) for oil and protein contents and their relationships with other seed quality traits in Brassica juncea[J]. Theoretical and Applied Genetics, 2006, 113: 1211-1220.
[68]Qiu D, Morgan J, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I. A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content[J]. Theoretical and Applied Genetics, 2006, 114: 67-80.
[69]邵玉锁,倪西源,黄吉祥,等.利用BC3F1群体定位和分析甘蓝型油菜A7-含油量QTL[J].中国农业科学, 2010,43(1):20-28.
[70]Hong D F, Liu J, Yang G S, He Q B. Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed[J]. Plant Breeding, 2008, 127: 69-73.
[71]Song L Q, Fu T D, Tu J X, Ma C Z, Yang G S. Molecular validation of multiple allele inheritance for dominant genic male sterility gene in Brassica napus L[J]. Theoretical and Applied Genetics, 2006, 113: 55- 62.
[72]高玉千,张书芬,朱家成,等.应用AFLP标记筛选甘蓝型油菜R4和22B的多态性[J].河南农业科学,2005, 8:32-35.
[73]Wasinger V C, Cordwell S J, Cerpa Poljak A, et al. Progress with gene produet mapping the mapping the Mollieutes: MyeoPlasma genitalium[J]. Electrophoresis, 1995, 16(7): 1090-1094.
[74]Wasinger V C, Cordewell S J, Cerpa-Poljak A, et al. Progress with gene-product mapping of the Mollicutes: MyeoPlasma geniitalium[J]. Electrophoresis, 1995, 16: 1090-1094.
[75]范海延,陈捷,曲波,等.蛋白质组学及其在植物科学研究中的应用[J].生物学通报,2006,41(l):6-8.
[76]孟慧,段翠芳,曾日中.植物蛋白质组学研究概况[J].热带农业科学,2006,26(2):60-65.
[77]Porubleva L, Velden K V, Kothari S, Oliver D J, Chitnis P R. The proteome of maize leaves: Use of gene sequences and expressed sequence tag data for identification of proteins with peptide mass fingerprints[J]. Electrophoresis, 2001, 22(9): 1724-1738.
[78]Wilson K A, McManusMargaret M T, Gordon E, Jordan T W. The proteomics of senescence in leaves of white clover, Trifolium repens(L.) [J]. Proteomics, 2002, 2(9): 1114-1122.
[79]Leonardi A, Damerval D, Vienne D. Organ-specific variability and inheritance of maize proteins revealed by two-dimensional electrophoresis[J]. Genet Res Camb, 1988, 52(1): 97-103.
[80]Bahrman N, Zivy M, Thiellement H. Genetic relationships in the sitopsis section of Triticum and the origin of the B genome of the polyploidy wheats[J]. Heredity, 1988, 61(6): 473-480.
[81]Nover L, Scharf K D. Synthesis,modification and structural binding of heat-shock proteins in tomato cell cultures[J]. Eur J Biochem, 1984, 139(2): 303-313.
[82]Majoul T, Bance E, Tribo Ee, Hamida J B, Branlard G.Proteomic analysis of the effect of heat stress on hexaploid wheat grain: Characterization of heat-responsive proteins from total endosperm[J]. Proteomics, 2003, 3(2): 175-183.
[83]Meza-Basso L, Alberdi M, Raynal M, Ferrero-Cadinanos M L, Delseny M. Changes in protein synthesis in rapeseed(Brassica napus)seedling during a low temperature treatment[J]. Plant Physiol, 1986, 82(3): 733- 738.
[84]Cui S X, Huang F, Wang J, Ma X, Cheng Y S, Liu J Y. A proteomic analysis of cold stress responses in rice seedlings[J]. Proteomics, 2005, 5(12): 3162-3172.
[85]Leymarie J, Damerval C, Marcotte L, Combes V, Vartanian N. two-dimensional protein patterns of Arabidopsis wild-type and auxin insensitive mutants, axr1, axr2,reveal interactions between drought and hormonal responses[J]. Plant Cell Physiol, 1996, 37(7): 966-975.
[86]Leone A, Costa A, Tucci M, Grillo S. Comparative analysis of short-and long-term changes in gene expression caused by low water potential in potato(Solanum tuberosum)cell suspension cultures[J]. Plant Physiol, 1994, 106(2): 703-712.
[87]Ramagopal S. Salinity stress induces tissue-specific proteins in barley seedlings[J]. Plant Physiol, 1987, 84(2): 324-331.
[88]Gerber S, Rodolphe F, Bahrman N, Baradat P. Seed-protein variation in maritime pine(Pinus pinaster Ait)revealed by two-dimensional electrophoresis: genetic determinism and construction of a linkage map [J]. Theoret Appl Genet, 1993, 85(5): 521-528.
[89]Vienne D, Leonardi A, Damerval C, Zivy M.Genetics of proteome variation as a tool for QTL characteri- zation. Application to drought stress responses in maize[J]. J Exp Bot, 1999, 50(332): 303-309.
[90]Nei M. Molecular Evolutionary Genetics[M]. New York: Columbia University Press, 1987.
[91]王学芳,孙万仓,李芳,等.中国西部冬油菜种植的生态效应评价[J].应用生态学报,2009,20(3): 647-652.
[92]郭海林,高雅丹,薛丹丹,等.结缕草属植物抗寒性的遗传分析[J].草业学报,2009,18(3):53-58.
[93]勃朗AG,莱尼REC,瞿梅HA(蒲富慎译).苹果、梨育种进展[J].北京:农业出版社,1981.117-120.
[94]Slushmoff C, Garley B. stushmoff, Breeding for cold hardiness[J]. in: Garley B. pear. New york: pleaun Press, 1990, 189-197.
[95]Charles L G. Freezing tolerance of plants: Current understanding and selected emerging concepts[J]. Canadian Journal of Botany, 2003, 81: 1216-1223.
[96]Philley H W, Watson C E, Krans J V. Inheritance of cold tolerance in St. Augustinegrass[J]. Crop Science,1998, 38: 451-454.
[97]王亚馥,粟翼玟,袁妙葆,等.遗传学[M].兰州大学出版社,1990,410-415.
[98]刘瞳,张学博.水稻对稻瘟病抗性的遗传研究——福建主要籼稻抗源的基因分析[J].植物病理学报, 1990, 20(1):41-46.
[99]陆光远,伍晓明,张冬晓,等.SSR标记分析国家油菜区试品种的特异性和一致性[J].中国农业科学,2008, 41(1):32-42.
[100]李丽,黄先群,Chokchai,等.AFLP与SSR标记及其在油菜研究中的应用[J].贵州农业科学,2009,37(11): 15-19.
[101]Haymes K. Mini-prep method suitable for a plant breeding program[J]. Plant Molecular Biology Report, 1996, 14: 280-284.
[102]陈德富,陈喜文.现代分子生物学实验原理与技术[M].北京,科学出版社,2006.
[103]王关林.植物基因工程[M].北京:科学出版社, 1998,608-609.
[104]吴乃虎.基因工程原理[M].科学出版社, 1998.
[105]Michelmore RW, Paran I, Kesseli RV. Identification of markers linked to disease-resistance genes by bulked segregant regions by using segregating populations[J]. Proceedings if the National Academy of Sciences, USA, 1991, 88: 9828-9832.
[106]刘仁虎,孟金陵. MapDraw,在Excel中绘制遗传连锁图的宏[J].遗传,2003,25(3):317-321.
[107]甘露,李殿荣,臧新,等.甘蓝型油菜蛋白质双向电泳体系的建立[J].作物学报,2010,36(4):612-619.
[108]刘海衡,,胡胜武,刘胜毅,等.油菜不同器官高质量总蛋白提取方法和双向电泳体系的优化[J].中国油料作物学报, 2009,31(4):426-433.
[109]皇甫海燕,官春云.甘蓝型油菜抗菌核病近等基因系和感病亲本蛋白差异的初步研究[J].中国农业科学,2010,43(10):2000-2007.
[110]熊志勇,夏伏建,陆师国.优质油菜籽粕蛋白质分类研究[J].武汉植物学研究,2001,19(3):259-261.
[111]孔芳,蒋金金,吴磊,等.油菜叶片总蛋白质双向电泳样品制备方法的改进[J].生物技术通报,2010,5: 93-96.
[112] Chen RZ, Weng QM, Huang Z, Zhu LL, He GC.Analysis of resistance-related proteins in rice against brown plantthopper by two-dimensional electrophoresis[J]. Acta Botanica Sinica, 2002, 44: 427-432.
[113]Damerval C, Vinne D, Zivy M, Thiellement H. Technical improvement in two-dimensional electrophoresis increase the level of genetic variation detected in wheat seedling proteins[J]. Electrop- horesis, 1986, 7: 52-54.
[114]汪家政,范明.蛋白质技术手册[M].北京:科学出版社,2004.
[2]孙万仓,马卫国,雷建民,等.冬油菜在西北旱寒区的适应性和北移的可行性研究[J].中国农业科学,2007, 40(12):2716-2726.
[3]朱惠霞,孙万仓,邓斌,等.白菜型冬油菜品种的抗寒性及其生理生化特性[J].西北农业学报,2007,16(4): 34-38.
[4]蒲媛媛,孙万仓.白菜型冬油菜抗寒性与生理生化特性关系[J].分子植物育种,2010,8(2),335-339.
[5]盖玥,牛俊义,孙万仓,等.降温处理对白菜型油菜品种抗寒生理指标的影响[J].甘肃农业大学学报,2005, 40(2)182-185.
[6]宋良图,郭书普.醋酶同工酶和过氧化物同工酶与油菜抗寒性的关系[J].安徽农业科学,1995,23(4): 333-335.
[7]苏维埃.植物对温度逆境的适应[A].植物生理与分子生物学[M].北京:科学出版社,2003:721-738.
[8]周瑞莲,赵哈林.高寒山区牧草根质膜和脂肪酸组分对冷冻低温的适应反应[J].植物生理学报,2001, 25(1):115-118.
[9]Deng L Y. Study on relationship between membrane lips fatty acid and winter hardy in grape[J]. Acta Phytophysiologia Sinica, 1982(8): 273-281.
[10]Murelli C F, Rizza G, Marinone F L. Metabolic changes associated with cold acclimation in contrasting cultivars of barley[J]. Physiologia plantarum, 1995, 94: 87-93.
[11]江勇,贾士荣,费云标.抗冻蛋白及其在植物抗冻生理中的作用[J].植物学报,1999,41(7):677-685.
[12]Fei Y B, Shu N H, Huang T, et al. Cellular and biochemical cor-relates to chilling and freezing tolerance in plant[J]. Prog Biotech, 1994, 14(3): 40-43.
[13]周丽英,杨丽涛,郑坚瑜.植物抗寒冻基因工程研究进展[J].植物学通报,2001,18(3):325-331.
[14]陈亚华,沈振国,刘友良.低温、高pH胁迫对水稻根系质膜、液泡膜ATP酶活性的影响[J].植物生理学报,2000,26(5):407-412.
[15]杨德浩,杨敏生,王进茂,等.欧洲白桦苗期低温胁迫时膜系统的变化[J].东北林业大学学报,2004,32(6): 13-15.
[16]艾琳,张萍,胡成志.低温胁迫对葡萄根系膜系统和可溶性糖及脯氨酸含量的影响[J].新疆农业大学学报,2004,27(4):47-50.
[17]P. H. LI. Plant Cold Tolerance and Freezing Stress[J]. New York, Academic Press, 1987: 37-48.
[18]Lyons, J. M. Chilling injury in plant[J]. Ann Rev Plant physiol, 1975, 24: 445-466.
[19]张泽煌,黄碧琦,陈钟佃,等.低温胁迫对茄子的伤害及茄子抗寒机理[J].福建农业学报,2000,15(1):40- 42.
[20]刘鸿先,曾韶西,王以柔,等.低温对不同耐寒力黄瓜幼苗子叶各细胞器官中SOD的影响[J].植物生理学报,1985,11(1):48-57.
[21]何若韫.植物低温逆境生理[M].北京:中国农业出版社,1995.
[22]马德华,卢育华,庞金安.低温对黄瓜幼苗膜质过氧化的影响[J].园艺学报,1998,25(1):61-64.
[23]张静.番茄苗期低温下生理生化特性的研究[D].硕士学位论文,扬州,扬州大学,2002.
[24]Chen H. H, Li D. H. Plant cold hardness and freezing stress[M]. Academic press, 1982, 5.
[25]谢吉容,向邓云,梅虎,等.南方红豆杉抗寒性的变化与内源激素的关系[J].西南师范大学学报(自然科学版), 2002, 27(2):231-234.
[26]Chen THH, Gusta LV. Abscisic acid-induced freezing re-sistance in cultured plant cells[J]. Plant Physiol, 1983, 73: 71-74.
[27]Violalang, E. T. Palva. Seasonal changes in tissue elasticity in chaparralshrubs[J]. Plant Physiol, 1994, 104(4): 1341-1349.
[28]梁建生.ABA对高等植物基因表达的调节作用[J].植物生理学通讯,1991,27(3):230-233.
[29]于贤昌,邢禹贤,马红,等.李滨低温胁迫下黄瓜嫁接苗和自根苗内源激素的变化[J].园艺学报,1999,26 (6):406-407.
[30]张荣华,李拥军,张叶玲.脯氨酸含量对苜蓿抗寒性影响的研究[J].现代化农业,2006,4:17-22.
[31]赵剑颖,宋晓莉,杨蕊,等.4℃胁迫过程中大叶黄杨和北海道黄杨叶片抗寒生理生化指标的变化[J].北京农学院学报,2010,25(2):57-61.
[32]王瑾,廖康,王燕凌,等.秋季自然降温对野生樱桃李叶片渗透调节物质的影响[J].新疆农业科学,2010, 47(5):952-957.
[33]任丽,岳桦. 4种中原牡丹在哈尔滨地区的抗寒性研究[J].植物研究,2010,30(2): 228-237.
[34]崔国文.紫花苜蓿田间越冬期抗寒生理研究[J].草地学报,2009,17(2):145-150.
[35]亓白岩,周冬琴,於朝广,等.8种含笑属植物的抗寒性研究[J].江苏农业科学,2010(5): 258-263.
[36]简令成,吴素萱.植物抗寒性的细胞学研究-小麦越冬过程中细胞内物质的变化[J].植物学报,1995(13): 1-16.
[37]郝丽娟,邵青玲.不同杏品种的抗寒性研究[J].山西果树,2006(5):3-5.
[38]马艳青,戴雄泽.低温胁迫对辣椒抗寒性相关生理指标的影响[J].湖南农业大学学报:自然科学版, 2000,26(6):461-462.
[39]郑荣梁.自由基生物学[M].北京:高等教育出版社,1992.
[40]王佩芝,符献琼,杜景智.冬小麦抗寒指标的研究[J].新疆农业科学,1997,4:18-22.
[41]孙学成,胡承孝,魏文学.钼肥对冬小麦脯氨酸及抗坏血酸含量的影响[J].三峡大学学报(自然科学版) 2002,23(5):474-477.
[42]李丽,黄先群, Chokchai Wanapu,等.贵州省2008年油菜区试材料的SSR遗传多样性分析[J].贵州农业科学,2010,38(11):1-4.
[43]唐容,王通强,黄泽素,等.利用SSR对甘蓝型黄籽油菜遗传多样性的研究[J].贵州农业科学,2009, 37(2): 7-10.
[44]伍宁丰,李汝刚,伍晓明,等.中国甘蓝型油菜遗传多样性的RAPD分子标记[J].生物多样性,1997,5(4):246-250.
[45]安贤惠,陈宝元,傅廷栋,等.利用RAPD标记研究中国芥菜型油菜遗传多样性[J].华中农业大学学报, 1999,18(6):524-527.
[46]李海渤,杨光圣,傅廷栋,等.部分甘蓝型油菜双低品种(系)的遗传多样性评估[J].韶关学院学报(自然科学版),2003,24(6):96-100.
[47]Diers B W, Osborn T C. Genetic diversity of oilseedBrassica napus germplasmbased on restriction fragment length polymorphisms[J].Theor Appl Genet, 1994, 88(6): 662-668.
[48]马朝芝,Sakai Takako,傅廷栋,等.RAPDs和RFLPs分析甘蓝型杂交油菜亲本的遗传多样性[J].作物学报,2003,29(5):701-707.
[49]曹维荣,王超.分子标记在甘蓝遗传育种中的应用[J].分子植物育种,2003, 4(1):547-550.
[50]Landry B. S, Hubert N, Etoh T, Harada J. J, and Lincoln S. E. A genetic map for Brassica napus based on restriction fragment length polymorphism detected with expressed DNA sequences[J]. Genome, 1991, 34 (4): 543-552.
[51]许鲲,张冬晓,伍晓明,等.国家冬油菜区试新品种SSR指纹图谱构建与遗传关系分析[J].中国油料作物学报,2008,30(1):29-34.
[52]雷天刚,张学昆,陆合,等.甘蓝型油菜黄籽品种SSR指纹图谱的构建[J].西南农业大学学报,2005,27(3): 361-364.
[53]刘平武,周国岭,杨光圣,等.双低甘蓝型油菜杂交种亲本指纹图谱构建和杂交种纯度鉴定[J].作物学报,2005,3l(5):640-646.
[54]唐容,王通强,黄泽素,等.利用SSR对甘蓝型黄籽油菜遗传多样性的研究[J].贵州农业科学,2009,37(2): 7-10.
[55]周红伟,姚艳梅,付忠,等.春性双低甘蓝型油菜杂交种和亲本指纹图谱构建及杂交种纯度鉴定[J].西北农业学报,2010,19(3):81-86.
[56]段院生,刘平武,杨光圣.甘蓝型油菜杂交种亲本指纹图谱分析[J].中国油料作物学报,2002,24(2):5-9.
[57]文雁成,王汉中,沈金雄,等.SRAP和SSR标记构建的甘蓝型油菜品种指纹图谱比较[J].中国油料作物学报,2006,28(3):233-239.
[58]林道哲,董顺文,刘朝辉,等.26份甘蓝型油菜自交系指纹图谱分析[J].安徽农业科学,2008,36(20): 8493-8496.
[59]陈碧云,伍晓明,张冬晓,等.国家冬油菜区试新品种的SSR指纹图谱分析[J].分子植物育种,2008,6(4): 709-716.
[60]钟军,李栒,官春云,等.中国芸芥栽培品种亲缘关系的RAPD分析[J].中国农业科学2004,37(10): 1434-1438.
[61]杨畅,郑普英,张学昆,等.甘蓝型油菜EST分子标记技术体系的建立[J].中国油料作物学报, 2008,30(1): 17-24.
[62]Hu J,. Quiros C, F. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 1991, 10: 505-511.
[63]刘杰,刘公社,李殿荣,等. RAPD方法鉴定油菜“杂油59”种子纯度的研究[J].应用与环境生物学报, 2000, 6(6):526-529.
[64]段院生,刘平武,杨光圣.用RAPD标记鉴定甘蓝型油菜杂种H9909的纯度[J].华中农业大学学报, 2002,21(4):301-305.
[65]汤天泽,肖小余,税红霞.SSR标记与形态学方法鉴定杂交油菜纯度的比较研究[J].分子植物育种, 2008,6(2):377-380.
[66]Burns M J, Barnes S R, Bowman J G, Clarke M H E, Werner C P, Kearsey M J. QTL analysis of an intervarietal set of substitution lines in Brassica napus:seed oil content and fatty acid composition[J]. Heredity, 2003, 90: 39-48.
[67]Mahmood T, Rahman M H, Stringam G R, Yeh F, Good A G. Identification of quantitative trait loci(QTL) for oil and protein contents and their relationships with other seed quality traits in Brassica juncea[J]. Theoretical and Applied Genetics, 2006, 113: 1211-1220.
[68]Qiu D, Morgan J, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I. A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content[J]. Theoretical and Applied Genetics, 2006, 114: 67-80.
[69]邵玉锁,倪西源,黄吉祥,等.利用BC3F1群体定位和分析甘蓝型油菜A7-含油量QTL[J].中国农业科学, 2010,43(1):20-28.
[70]Hong D F, Liu J, Yang G S, He Q B. Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed[J]. Plant Breeding, 2008, 127: 69-73.
[71]Song L Q, Fu T D, Tu J X, Ma C Z, Yang G S. Molecular validation of multiple allele inheritance for dominant genic male sterility gene in Brassica napus L[J]. Theoretical and Applied Genetics, 2006, 113: 55- 62.
[72]高玉千,张书芬,朱家成,等.应用AFLP标记筛选甘蓝型油菜R4和22B的多态性[J].河南农业科学,2005, 8:32-35.
[73]Wasinger V C, Cordwell S J, Cerpa Poljak A, et al. Progress with gene produet mapping the mapping the Mollieutes: MyeoPlasma genitalium[J]. Electrophoresis, 1995, 16(7): 1090-1094.
[74]Wasinger V C, Cordewell S J, Cerpa-Poljak A, et al. Progress with gene-product mapping of the Mollicutes: MyeoPlasma geniitalium[J]. Electrophoresis, 1995, 16: 1090-1094.
[75]范海延,陈捷,曲波,等.蛋白质组学及其在植物科学研究中的应用[J].生物学通报,2006,41(l):6-8.
[76]孟慧,段翠芳,曾日中.植物蛋白质组学研究概况[J].热带农业科学,2006,26(2):60-65.
[77]Porubleva L, Velden K V, Kothari S, Oliver D J, Chitnis P R. The proteome of maize leaves: Use of gene sequences and expressed sequence tag data for identification of proteins with peptide mass fingerprints[J]. Electrophoresis, 2001, 22(9): 1724-1738.
[78]Wilson K A, McManusMargaret M T, Gordon E, Jordan T W. The proteomics of senescence in leaves of white clover, Trifolium repens(L.) [J]. Proteomics, 2002, 2(9): 1114-1122.
[79]Leonardi A, Damerval D, Vienne D. Organ-specific variability and inheritance of maize proteins revealed by two-dimensional electrophoresis[J]. Genet Res Camb, 1988, 52(1): 97-103.
[80]Bahrman N, Zivy M, Thiellement H. Genetic relationships in the sitopsis section of Triticum and the origin of the B genome of the polyploidy wheats[J]. Heredity, 1988, 61(6): 473-480.
[81]Nover L, Scharf K D. Synthesis,modification and structural binding of heat-shock proteins in tomato cell cultures[J]. Eur J Biochem, 1984, 139(2): 303-313.
[82]Majoul T, Bance E, Tribo Ee, Hamida J B, Branlard G.Proteomic analysis of the effect of heat stress on hexaploid wheat grain: Characterization of heat-responsive proteins from total endosperm[J]. Proteomics, 2003, 3(2): 175-183.
[83]Meza-Basso L, Alberdi M, Raynal M, Ferrero-Cadinanos M L, Delseny M. Changes in protein synthesis in rapeseed(Brassica napus)seedling during a low temperature treatment[J]. Plant Physiol, 1986, 82(3): 733- 738.
[84]Cui S X, Huang F, Wang J, Ma X, Cheng Y S, Liu J Y. A proteomic analysis of cold stress responses in rice seedlings[J]. Proteomics, 2005, 5(12): 3162-3172.
[85]Leymarie J, Damerval C, Marcotte L, Combes V, Vartanian N. two-dimensional protein patterns of Arabidopsis wild-type and auxin insensitive mutants, axr1, axr2,reveal interactions between drought and hormonal responses[J]. Plant Cell Physiol, 1996, 37(7): 966-975.
[86]Leone A, Costa A, Tucci M, Grillo S. Comparative analysis of short-and long-term changes in gene expression caused by low water potential in potato(Solanum tuberosum)cell suspension cultures[J]. Plant Physiol, 1994, 106(2): 703-712.
[87]Ramagopal S. Salinity stress induces tissue-specific proteins in barley seedlings[J]. Plant Physiol, 1987, 84(2): 324-331.
[88]Gerber S, Rodolphe F, Bahrman N, Baradat P. Seed-protein variation in maritime pine(Pinus pinaster Ait)revealed by two-dimensional electrophoresis: genetic determinism and construction of a linkage map [J]. Theoret Appl Genet, 1993, 85(5): 521-528.
[89]Vienne D, Leonardi A, Damerval C, Zivy M.Genetics of proteome variation as a tool for QTL characteri- zation. Application to drought stress responses in maize[J]. J Exp Bot, 1999, 50(332): 303-309.
[90]Nei M. Molecular Evolutionary Genetics[M]. New York: Columbia University Press, 1987.
[91]王学芳,孙万仓,李芳,等.中国西部冬油菜种植的生态效应评价[J].应用生态学报,2009,20(3): 647-652.
[92]郭海林,高雅丹,薛丹丹,等.结缕草属植物抗寒性的遗传分析[J].草业学报,2009,18(3):53-58.
[93]勃朗AG,莱尼REC,瞿梅HA(蒲富慎译).苹果、梨育种进展[J].北京:农业出版社,1981.117-120.
[94]Slushmoff C, Garley B. stushmoff, Breeding for cold hardiness[J]. in: Garley B. pear. New york: pleaun Press, 1990, 189-197.
[95]Charles L G. Freezing tolerance of plants: Current understanding and selected emerging concepts[J]. Canadian Journal of Botany, 2003, 81: 1216-1223.
[96]Philley H W, Watson C E, Krans J V. Inheritance of cold tolerance in St. Augustinegrass[J]. Crop Science,1998, 38: 451-454.
[97]王亚馥,粟翼玟,袁妙葆,等.遗传学[M].兰州大学出版社,1990,410-415.
[98]刘瞳,张学博.水稻对稻瘟病抗性的遗传研究——福建主要籼稻抗源的基因分析[J].植物病理学报, 1990, 20(1):41-46.
[99]陆光远,伍晓明,张冬晓,等.SSR标记分析国家油菜区试品种的特异性和一致性[J].中国农业科学,2008, 41(1):32-42.
[100]李丽,黄先群,Chokchai,等.AFLP与SSR标记及其在油菜研究中的应用[J].贵州农业科学,2009,37(11): 15-19.
[101]Haymes K. Mini-prep method suitable for a plant breeding program[J]. Plant Molecular Biology Report, 1996, 14: 280-284.
[102]陈德富,陈喜文.现代分子生物学实验原理与技术[M].北京,科学出版社,2006.
[103]王关林.植物基因工程[M].北京:科学出版社, 1998,608-609.
[104]吴乃虎.基因工程原理[M].科学出版社, 1998.
[105]Michelmore RW, Paran I, Kesseli RV. Identification of markers linked to disease-resistance genes by bulked segregant regions by using segregating populations[J]. Proceedings if the National Academy of Sciences, USA, 1991, 88: 9828-9832.
[106]刘仁虎,孟金陵. MapDraw,在Excel中绘制遗传连锁图的宏[J].遗传,2003,25(3):317-321.
[107]甘露,李殿荣,臧新,等.甘蓝型油菜蛋白质双向电泳体系的建立[J].作物学报,2010,36(4):612-619.
[108]刘海衡,,胡胜武,刘胜毅,等.油菜不同器官高质量总蛋白提取方法和双向电泳体系的优化[J].中国油料作物学报, 2009,31(4):426-433.
[109]皇甫海燕,官春云.甘蓝型油菜抗菌核病近等基因系和感病亲本蛋白差异的初步研究[J].中国农业科学,2010,43(10):2000-2007.
[110]熊志勇,夏伏建,陆师国.优质油菜籽粕蛋白质分类研究[J].武汉植物学研究,2001,19(3):259-261.
[111]孔芳,蒋金金,吴磊,等.油菜叶片总蛋白质双向电泳样品制备方法的改进[J].生物技术通报,2010,5: 93-96.
[112] Chen RZ, Weng QM, Huang Z, Zhu LL, He GC.Analysis of resistance-related proteins in rice against brown plantthopper by two-dimensional electrophoresis[J]. Acta Botanica Sinica, 2002, 44: 427-432.
[113]Damerval C, Vinne D, Zivy M, Thiellement H. Technical improvement in two-dimensional electrophoresis increase the level of genetic variation detected in wheat seedling proteins[J]. Electrop- horesis, 1986, 7: 52-54.
[114]汪家政,范明.蛋白质技术手册[M].北京:科学出版社,2004.