中药补脑止痫散对癫痫大鼠海马c-fos、PKCa表达和谷氨酸、γ-氨基丁酸含量的影响
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摘要
目的
研究戊四氮致痫大鼠在使用补脑止痫散治疗前后一般状态、行为学海马组织病理学以及海马内c-fos、PKCa表达和谷氨酸(Glu)、γ-氨基丁酸(GABA)含量的变化情况,探讨补脑止痫散的抗痫机制,为该方的临床应用提供参考依据
方法
成年Wistar大鼠100只,随机选取10只作为空白组,其余90只用于癫痫模型点燃。点燃成功的大鼠随机平均分配到以下7组：模型组、补脑止痫散低、中、高剂量10天组以及低、中、高剂量21天组。在开始灌胃治疗的前1天以及疗程中的第10和21天观察相应组别大鼠的一般状态并称取体质量,记录痫性发作潜伏期和发作级别。HE染色观察海马组织病理学变化情况。免疫组化法检测海马c-fos、PKCa、Glu、GABA阳性细胞数,计算阳性细胞的平均灰度值；并计算Glu和GABA阳性细胞数的比值以及二者平均灰度值的比值。实验数据均输入SPSS17.0进行统计学处理,P<0.05为有显著差异,P<0.01为有极显著差异。
结果
共有70只大鼠成功点燃。灌胃过程中,低、中剂量10天组各死亡1只,模型组和中、高剂量21天组各死亡2只
1一般状态和体质量变化情况
模型组大鼠一般状态明显较空白组差,补脑止痫散各组与模型组相比均有改善,中、高剂量组好于低剂量组,21天组好于10天组。
治疗前,模型组、补脑止痫散各组大鼠的体质量与空白组相比均明显变小(均为P<0.01)。治疗10天后,与模型组相比,低剂量组无显著变化(P>0.05),而中、高剂量组明显增加(均为P<0.01)。治疗21天后,补脑止痫散各组的体质量与模型组相比均明显增加(均为P<0.01)。
2行为学观察
空白组无痫性发作。灌胃前1天,模型组和补脑止痫散各组均可出现Racine4级或以上发作。治疗10天后,与模型组相比,低剂量组发作潜伏期无显著变化(P>0.05),但发作级别明显降低(P<0.05)；而中、高剂量组的发作潜伏期均明显延长,发作级别均明显降低(均为P<0.01)。治疗21天后,与模型组相比,补脑止痫散各组的发作潜伏期均明显延长,发作级别均明显降低(P<0.05或P<0.01)。
3HE染色
空白组大鼠海马细胞数量和结构均正常。模型组细胞数量明显减少,层次紊乱,细胞肿胀变形,胞浆染色加深,胞核固缩、碎裂。补脑止痫散各组较模型组均有改善,中、高剂量组的改善程度大于低剂量组,21天组大于10天组。
4c-fos的表达情况
空白组c-fos阳性细胞很少,平均灰度值最高,与之相比模型组阳性细胞数明显增多,平均灰度值明显降低(均为P<0.01)。与模型组相比,除低剂量10天组外,补脑止痫散各组阳性细胞数均明显减少,平均灰度值均明显升高(P<0.05或P<0.01)。无论是阳性细胞数还是平均灰度值,疗程相同的低、中、高剂量组之间两两比较均有极显著差异。剂量相同的10天组和21天组之间阳性细胞数均无显著差异,而平均灰度值均有显著差异。
5PKCa的表达情况
阳性细胞数方面,空白组很少,与之相比模型组明显增多(P<0.01)。与模型组相比,补脑止痫散各组均明显减少(P<0.05或P<0.01)。低、中高剂量10天组之间均有显著或极显著差异。低、中剂量21天组之间有极显著差异,中、高剂量21天组之间无显著差异。平均灰度值方面,模型组较空白组明显降低(P<0.01)。与模型组相比,除低剂量10天组外,补脑止痫散各组均明显升高(P<0.05或P<0.01)。疗程相同的低、中剂量组之间均有显著差异,中、高剂量组之间均无显著差异。无论是阳性细胞数还是平均灰度值,剂量相同的10天组和21天组之间均无显著差异
6Glu和GABA的含量变化情况
空白组Gl u阳性细胞数量最少,平均灰度值最高；GABA阳性细胞数量最多,平均灰度值最低。与之相比模型组Glu阳性细胞数明显增多,平均灰度值明显降低：GABA阳性细胞数明显减少,平均灰度值明显升高(均为P<0.01)。与模型组相比,除低剂量10天组外,补脑止痫散各组的Glu阳性细胞数均明显减少,平均灰度值均明显升高；GABA阳性细胞数均明显增多,平均灰度值均明显降低(P<0.05或P<0.01)。无论是Glu和GABA的阳性细胞数还是二者的平均灰度值,疗程相同的低、中剂量组之间均有显著或极显著差异,而中、高剂量组之间均无显著差异；剂量相同的10天组和21天组之间均无显著差异。
根据秩均值,Glu和GABA阳性细胞数的比值在空白组最小,模型组最大。与模型组相比,补脑止痫散各组均明显变小,且高剂量组小于中剂量组,中剂量组小于低剂量组,21天组小于10天组。Glu和GABA平均灰度值的比值在各组之间的变化趋势与阳性细胞数的比值相反。
结论
1、补脑止痫散能明显改善癫痫大鼠的一般状态,纠正其体质量减轻的趋势；中、高剂量组作用强于低剂量组,21天组强于10天组。
2、本方能明显延长癫痫大鼠的发作潜伏期,降低其痫性发作级别；中、高剂量组作用强于低剂量组,21天组略强于10天组。
3、本方能明显减轻癫痫大鼠海马神经元的病理损伤；中、高剂量组作用强于低剂量组,21天组强于10天组。
4、本方能明显抑制癫痫大鼠海马c-fos的表达；高剂量组作用最强中剂量组次之,低剂量组最弱,21天组略强于10天组
5、本方能明显抑制癫痫大鼠海马PKCa的表达；中、高剂量组作用强十低剂量组,21天组与10天组相当。
6、本方能降低癫痫大鼠海马G1u含量,提高GABA含量,纠正二者的失衡状态；中、高剂量组作用强于低剂量组,21天组略强于10天组。
Obj ect i ve:
We induced kindling epileptic rats using pentylenetetrazole, observed their changes in general state, behavioristics and hippocampal pathology, examined the changes in the expression of c-fos, PKCa and in the contents of Glutamate(Glu), GABA in the hippocampus of them before and after they were treated with the Bunaozhixian powder. According to the experimental results, we explored the antiepileptic mechanism of this Chinese medicinal formule, so as to provide reference basis for its clinical use.
Methods:
10rats were randomly chosen from100adult Wistar ones to make up the blank group, and the remaining90were used to induce epileptic models by PTZ. The kindled rats were randomly divided into7groups:the model group, the low, medium and high Bunaozhixian powder groups treated for10days and the low, medium and high Bunaozhixian powder groups treated for21days. All the rats in the Bunaozhixian powder groups were intragastrically treated with Bunaozhixian powder with certain dosages. We observed the general state of the rats in certain groups and weighed them on the day before the treatment was given and the10th,21st day of the treatment. Meanwhile, their incubation period and seizure grade were also recorded. The hippocampal slices were stained using HE, in this way the hippocampal pathological changes were observed. Meanwhile, we examined the positive cell numbers of c-fos, PKCa, Glu and GABA, and calculated the average grey values by immunohistochemistry. At the same time, we calculated the ratios of the positive cell number of Glu and GABA, as well as the ratios of the grey values of them. The experimental data were input into SPSS17.0for statistical process, P<0.05was considered to be significant difference and P<0.01to be very significant difference.
Results:
70rats were successfully kindled. During the treatment course.1rat in the10-day low dosage group died, as well as in the10-day medium group.2rats in the model group died, as well as in the21-day medium and high dosage group.
1The changes in general state and weight
The rats'general state was apparently worse in the model group than in the blank. Compared with the model, it improved obviously in the Bunaozhixian powder groups, and the effects of the medium and high dosage groups are more obvious than the low, as well as the21-day groups are than the10-day ones.
On the day before the treatment was given, compared with the blank group, weight of the rats in the model and all the Bunaozhixian powder groups lightened very significantly (P<0.01). On the10th day of the treatment course, compared with the model group, it increased very significantly in the medium and high dosage group (P<0.01), but it had no significant change in the low (P>0.05). On the21st day. weight of the rats in all the Bunaozhixian powder groups increased very significantly (P<0.01).
2The changes in behavioristics
Rats in the blank group had no seizures. On the day before the treatment was given, all the rats' seizures in the model and the Bunaozhixian powder groups reached4grade or higher according to the Racine scale. On the10th day, compared with the model group, the incubation period of the low dosage group has no significant change (P>0.05). but its seizure grade decreased significantly (P<0.05). The incubation period of the medium and high lengthened and their seizure grade decreased, the differences were both very significant (P<0.01). On the21st day. the incubation period of all the Bunaozhixian powder groups lengthened and their seizure grade decreased, the differences were significant or very significant (P<0.05or P<0.01).
3Hippocampal pathological changes
Microscopically, in the blank group, the number and structure of neurons were normal in the hippocampus. However, the situation in the model group was quite different:the number of neurons decreased obviously, their arrangement was in disorder. The neurons swelled and their cytoplasms were stained darker than normal, their nucleuses were pyknotic and cataclastic. Compared with the model group, the situation in the Bunaozhixian powder groups improved apparently, the effect of the medium and high dosage groups are more obvious than the low, and the21-day groups are more obvious than the10-day ones.
4The expression of c-fos
In the blank group, only a few c-fos positive cells could be found, and the average grey value was highest. Compared with that, in the model group, positive cells increased and the grey value lowered, the differences were both very significant (P<0.01). Compared with the model group, positive cells decreased and the grey value rose in the Bunaozhixian powder groups except the10-day low dosage group, the differences were significant or very significant (P<0.05or P<0.01).
Comparisons among the groups treated with the same course, either in positive cell number or in average grey value, there were very significant differences among the low, the medium and the high dosage groups. Comparisons between the groups treated with the same dosage, there was no significant difference in positive cell number between the10-day groups and the21-day ones, but there were significant differences in average grey value between them.
5The expression of PKCa
There were only a few PKCa positive cells in the blank group. Compared with that, it increased very significantly in the model group (P<0.01). Compared with the model group, it decreased in the Bunaozhixian powder groups, the differences were significant or very significant (P<0.05or P<0.01). Comparisons among the10-day groups, the differences were all significant or very significant. Comparisons among the21-day groups, there was very significant difference between the low and the medium dosage group. but the difference between the medium and the high was not significant.
The average grey value was highest in the blank group, compared with that, in the model group, it lowered very significantly (P<0.01). Compared with the model group, it rose in the Bunaozhixian powder groups except the10-day low dosage one, the differences were significant or very significant (P<0.05or P<0.01). Comparisons among the groups treated with the same course, there were significant differences between the low and medium dosage groups, but there was no significant difference between the medium and the high.
Comparisons between the groups treated with the same dosage, either in positive cell number or in average grey value, there was no significant difference between the10-day groups and the21-day ones.6The contents of Glu and GABA
In the blank group, there were only a few Glu positive cells, and the average grey value was highest; while the number of GABA positive cells was largest and the average grey value was lowest. Compared with that, in the model group, the positive cells of Glu increased and the grey value lowered; the number of GABA positive cells decreased and the grey value rose, the differences were all very significant (P<0.01). Compared with the model group, the positive cells of Glu decreased and the grey value rose, the positive cells of GABA increased and the grey value lowered in the Bunaozhixian powder groups, except the10-day low dosage one; the differences were significant or very significant (P<0.05or P<0.01).
Comparisons among the groups treated with the same course, either in positive cell number or in average grey value, the differences between the low and the medium dosage groups were significant or very significant, but the differences between the medium and the high were not significant. Comparisons between the groups treated with the same dosage, either in positive cell number or in average grey value, there was no significant difference between the10-day groups and the21-day ones.
According to the mean ranks, the ratio of Glu and GABA positive cell numbers was smallest in the blank group and was largest in the model. Compared with the model group, it decreased in all the Bunaozhixian powder groups. It was smaller in the high dosage groups than in the medium, as well as in the medium was than in the low; meanwhile, it was smaller in the21-day groups than in the10-day ones. The variation tendency of the ratio of the average grey value among the the Bunaozhixian powder groups was just the opposite.
Conclusions:
1The Bunaozhixian powder can obviously improve the general state of epileptic rats and remedy their trend of losing weight. The effects of the medium and high dosage groups are more significant than the low, as well as the21-day groups are than the10-day ones.
2It can obviously lengthen the seizure incubation period of epileptic rats and decrease their seizure grade. The effects of the medium and high dosage groups are more significant than the low. and the21-day groups are a little more significant than the10-day ones.
3It can obviously alleviate the neuronal injuries in epileptic rats' hippocampus. The effects of the medium and high dosage groups are more significant than the low, as well as the21-day groups are than the10-day ones.
4It can obviously inhibit the expression of c-fos in hippocampus of epileptic rats. The effects of the high dosage groups are more significant than the medium, as well as the medium are than the low. The21-day groups are a little more significant than the10-day ones.
5It can obviously inhibit the expression of PKCa in hippocampus of epileptic rats. The effects of the medium and high dosage groups are more significant than the low. but there is no obvious difference between the21-day groups and the10-day ones.
6It can reduce the contents of Glu and increase that of GABA in hippocampus of epileptic rats, so as to remedy their unbalance conditions. The effects of the medium and high dosage groups are more significant than the low; the21-day groups are a little more significant than the10-day ones.
研究戊四氮致痫大鼠在使用补脑止痫散治疗前后一般状态、行为学海马组织病理学以及海马内c-fos、PKCa表达和谷氨酸(Glu)、γ-氨基丁酸(GABA)含量的变化情况,探讨补脑止痫散的抗痫机制,为该方的临床应用提供参考依据
方法
成年Wistar大鼠100只,随机选取10只作为空白组,其余90只用于癫痫模型点燃。点燃成功的大鼠随机平均分配到以下7组：模型组、补脑止痫散低、中、高剂量10天组以及低、中、高剂量21天组。在开始灌胃治疗的前1天以及疗程中的第10和21天观察相应组别大鼠的一般状态并称取体质量,记录痫性发作潜伏期和发作级别。HE染色观察海马组织病理学变化情况。免疫组化法检测海马c-fos、PKCa、Glu、GABA阳性细胞数,计算阳性细胞的平均灰度值；并计算Glu和GABA阳性细胞数的比值以及二者平均灰度值的比值。实验数据均输入SPSS17.0进行统计学处理,P<0.05为有显著差异,P<0.01为有极显著差异。
结果
共有70只大鼠成功点燃。灌胃过程中,低、中剂量10天组各死亡1只,模型组和中、高剂量21天组各死亡2只
1一般状态和体质量变化情况
模型组大鼠一般状态明显较空白组差,补脑止痫散各组与模型组相比均有改善,中、高剂量组好于低剂量组,21天组好于10天组。
治疗前,模型组、补脑止痫散各组大鼠的体质量与空白组相比均明显变小(均为P<0.01)。治疗10天后,与模型组相比,低剂量组无显著变化(P>0.05),而中、高剂量组明显增加(均为P<0.01)。治疗21天后,补脑止痫散各组的体质量与模型组相比均明显增加(均为P<0.01)。
2行为学观察
空白组无痫性发作。灌胃前1天,模型组和补脑止痫散各组均可出现Racine4级或以上发作。治疗10天后,与模型组相比,低剂量组发作潜伏期无显著变化(P>0.05),但发作级别明显降低(P<0.05)；而中、高剂量组的发作潜伏期均明显延长,发作级别均明显降低(均为P<0.01)。治疗21天后,与模型组相比,补脑止痫散各组的发作潜伏期均明显延长,发作级别均明显降低(P<0.05或P<0.01)。
3HE染色
空白组大鼠海马细胞数量和结构均正常。模型组细胞数量明显减少,层次紊乱,细胞肿胀变形,胞浆染色加深,胞核固缩、碎裂。补脑止痫散各组较模型组均有改善,中、高剂量组的改善程度大于低剂量组,21天组大于10天组。
4c-fos的表达情况
空白组c-fos阳性细胞很少,平均灰度值最高,与之相比模型组阳性细胞数明显增多,平均灰度值明显降低(均为P<0.01)。与模型组相比,除低剂量10天组外,补脑止痫散各组阳性细胞数均明显减少,平均灰度值均明显升高(P<0.05或P<0.01)。无论是阳性细胞数还是平均灰度值,疗程相同的低、中、高剂量组之间两两比较均有极显著差异。剂量相同的10天组和21天组之间阳性细胞数均无显著差异,而平均灰度值均有显著差异。
5PKCa的表达情况
阳性细胞数方面,空白组很少,与之相比模型组明显增多(P<0.01)。与模型组相比,补脑止痫散各组均明显减少(P<0.05或P<0.01)。低、中高剂量10天组之间均有显著或极显著差异。低、中剂量21天组之间有极显著差异,中、高剂量21天组之间无显著差异。平均灰度值方面,模型组较空白组明显降低(P<0.01)。与模型组相比,除低剂量10天组外,补脑止痫散各组均明显升高(P<0.05或P<0.01)。疗程相同的低、中剂量组之间均有显著差异,中、高剂量组之间均无显著差异。无论是阳性细胞数还是平均灰度值,剂量相同的10天组和21天组之间均无显著差异
6Glu和GABA的含量变化情况
空白组Gl u阳性细胞数量最少,平均灰度值最高；GABA阳性细胞数量最多,平均灰度值最低。与之相比模型组Glu阳性细胞数明显增多,平均灰度值明显降低：GABA阳性细胞数明显减少,平均灰度值明显升高(均为P<0.01)。与模型组相比,除低剂量10天组外,补脑止痫散各组的Glu阳性细胞数均明显减少,平均灰度值均明显升高；GABA阳性细胞数均明显增多,平均灰度值均明显降低(P<0.05或P<0.01)。无论是Glu和GABA的阳性细胞数还是二者的平均灰度值,疗程相同的低、中剂量组之间均有显著或极显著差异,而中、高剂量组之间均无显著差异；剂量相同的10天组和21天组之间均无显著差异。
根据秩均值,Glu和GABA阳性细胞数的比值在空白组最小,模型组最大。与模型组相比,补脑止痫散各组均明显变小,且高剂量组小于中剂量组,中剂量组小于低剂量组,21天组小于10天组。Glu和GABA平均灰度值的比值在各组之间的变化趋势与阳性细胞数的比值相反。
结论
1、补脑止痫散能明显改善癫痫大鼠的一般状态,纠正其体质量减轻的趋势；中、高剂量组作用强于低剂量组,21天组强于10天组。
2、本方能明显延长癫痫大鼠的发作潜伏期,降低其痫性发作级别；中、高剂量组作用强于低剂量组,21天组略强于10天组。
3、本方能明显减轻癫痫大鼠海马神经元的病理损伤；中、高剂量组作用强于低剂量组,21天组强于10天组。
4、本方能明显抑制癫痫大鼠海马c-fos的表达；高剂量组作用最强中剂量组次之,低剂量组最弱,21天组略强于10天组
5、本方能明显抑制癫痫大鼠海马PKCa的表达；中、高剂量组作用强十低剂量组,21天组与10天组相当。
6、本方能降低癫痫大鼠海马G1u含量,提高GABA含量,纠正二者的失衡状态；中、高剂量组作用强于低剂量组,21天组略强于10天组。
Obj ect i ve:
We induced kindling epileptic rats using pentylenetetrazole, observed their changes in general state, behavioristics and hippocampal pathology, examined the changes in the expression of c-fos, PKCa and in the contents of Glutamate(Glu), GABA in the hippocampus of them before and after they were treated with the Bunaozhixian powder. According to the experimental results, we explored the antiepileptic mechanism of this Chinese medicinal formule, so as to provide reference basis for its clinical use.
Methods:
10rats were randomly chosen from100adult Wistar ones to make up the blank group, and the remaining90were used to induce epileptic models by PTZ. The kindled rats were randomly divided into7groups:the model group, the low, medium and high Bunaozhixian powder groups treated for10days and the low, medium and high Bunaozhixian powder groups treated for21days. All the rats in the Bunaozhixian powder groups were intragastrically treated with Bunaozhixian powder with certain dosages. We observed the general state of the rats in certain groups and weighed them on the day before the treatment was given and the10th,21st day of the treatment. Meanwhile, their incubation period and seizure grade were also recorded. The hippocampal slices were stained using HE, in this way the hippocampal pathological changes were observed. Meanwhile, we examined the positive cell numbers of c-fos, PKCa, Glu and GABA, and calculated the average grey values by immunohistochemistry. At the same time, we calculated the ratios of the positive cell number of Glu and GABA, as well as the ratios of the grey values of them. The experimental data were input into SPSS17.0for statistical process, P<0.05was considered to be significant difference and P<0.01to be very significant difference.
Results:
70rats were successfully kindled. During the treatment course.1rat in the10-day low dosage group died, as well as in the10-day medium group.2rats in the model group died, as well as in the21-day medium and high dosage group.
1The changes in general state and weight
The rats'general state was apparently worse in the model group than in the blank. Compared with the model, it improved obviously in the Bunaozhixian powder groups, and the effects of the medium and high dosage groups are more obvious than the low, as well as the21-day groups are than the10-day ones.
On the day before the treatment was given, compared with the blank group, weight of the rats in the model and all the Bunaozhixian powder groups lightened very significantly (P<0.01). On the10th day of the treatment course, compared with the model group, it increased very significantly in the medium and high dosage group (P<0.01), but it had no significant change in the low (P>0.05). On the21st day. weight of the rats in all the Bunaozhixian powder groups increased very significantly (P<0.01).
2The changes in behavioristics
Rats in the blank group had no seizures. On the day before the treatment was given, all the rats' seizures in the model and the Bunaozhixian powder groups reached4grade or higher according to the Racine scale. On the10th day, compared with the model group, the incubation period of the low dosage group has no significant change (P>0.05). but its seizure grade decreased significantly (P<0.05). The incubation period of the medium and high lengthened and their seizure grade decreased, the differences were both very significant (P<0.01). On the21st day. the incubation period of all the Bunaozhixian powder groups lengthened and their seizure grade decreased, the differences were significant or very significant (P<0.05or P<0.01).
3Hippocampal pathological changes
Microscopically, in the blank group, the number and structure of neurons were normal in the hippocampus. However, the situation in the model group was quite different:the number of neurons decreased obviously, their arrangement was in disorder. The neurons swelled and their cytoplasms were stained darker than normal, their nucleuses were pyknotic and cataclastic. Compared with the model group, the situation in the Bunaozhixian powder groups improved apparently, the effect of the medium and high dosage groups are more obvious than the low, and the21-day groups are more obvious than the10-day ones.
4The expression of c-fos
In the blank group, only a few c-fos positive cells could be found, and the average grey value was highest. Compared with that, in the model group, positive cells increased and the grey value lowered, the differences were both very significant (P<0.01). Compared with the model group, positive cells decreased and the grey value rose in the Bunaozhixian powder groups except the10-day low dosage group, the differences were significant or very significant (P<0.05or P<0.01).
Comparisons among the groups treated with the same course, either in positive cell number or in average grey value, there were very significant differences among the low, the medium and the high dosage groups. Comparisons between the groups treated with the same dosage, there was no significant difference in positive cell number between the10-day groups and the21-day ones, but there were significant differences in average grey value between them.
5The expression of PKCa
There were only a few PKCa positive cells in the blank group. Compared with that, it increased very significantly in the model group (P<0.01). Compared with the model group, it decreased in the Bunaozhixian powder groups, the differences were significant or very significant (P<0.05or P<0.01). Comparisons among the10-day groups, the differences were all significant or very significant. Comparisons among the21-day groups, there was very significant difference between the low and the medium dosage group. but the difference between the medium and the high was not significant.
The average grey value was highest in the blank group, compared with that, in the model group, it lowered very significantly (P<0.01). Compared with the model group, it rose in the Bunaozhixian powder groups except the10-day low dosage one, the differences were significant or very significant (P<0.05or P<0.01). Comparisons among the groups treated with the same course, there were significant differences between the low and medium dosage groups, but there was no significant difference between the medium and the high.
Comparisons between the groups treated with the same dosage, either in positive cell number or in average grey value, there was no significant difference between the10-day groups and the21-day ones.6The contents of Glu and GABA
In the blank group, there were only a few Glu positive cells, and the average grey value was highest; while the number of GABA positive cells was largest and the average grey value was lowest. Compared with that, in the model group, the positive cells of Glu increased and the grey value lowered; the number of GABA positive cells decreased and the grey value rose, the differences were all very significant (P<0.01). Compared with the model group, the positive cells of Glu decreased and the grey value rose, the positive cells of GABA increased and the grey value lowered in the Bunaozhixian powder groups, except the10-day low dosage one; the differences were significant or very significant (P<0.05or P<0.01).
Comparisons among the groups treated with the same course, either in positive cell number or in average grey value, the differences between the low and the medium dosage groups were significant or very significant, but the differences between the medium and the high were not significant. Comparisons between the groups treated with the same dosage, either in positive cell number or in average grey value, there was no significant difference between the10-day groups and the21-day ones.
According to the mean ranks, the ratio of Glu and GABA positive cell numbers was smallest in the blank group and was largest in the model. Compared with the model group, it decreased in all the Bunaozhixian powder groups. It was smaller in the high dosage groups than in the medium, as well as in the medium was than in the low; meanwhile, it was smaller in the21-day groups than in the10-day ones. The variation tendency of the ratio of the average grey value among the the Bunaozhixian powder groups was just the opposite.
Conclusions:
1The Bunaozhixian powder can obviously improve the general state of epileptic rats and remedy their trend of losing weight. The effects of the medium and high dosage groups are more significant than the low, as well as the21-day groups are than the10-day ones.
2It can obviously lengthen the seizure incubation period of epileptic rats and decrease their seizure grade. The effects of the medium and high dosage groups are more significant than the low. and the21-day groups are a little more significant than the10-day ones.
3It can obviously alleviate the neuronal injuries in epileptic rats' hippocampus. The effects of the medium and high dosage groups are more significant than the low, as well as the21-day groups are than the10-day ones.
4It can obviously inhibit the expression of c-fos in hippocampus of epileptic rats. The effects of the high dosage groups are more significant than the medium, as well as the medium are than the low. The21-day groups are a little more significant than the10-day ones.
5It can obviously inhibit the expression of PKCa in hippocampus of epileptic rats. The effects of the medium and high dosage groups are more significant than the low. but there is no obvious difference between the21-day groups and the10-day ones.
6It can reduce the contents of Glu and increase that of GABA in hippocampus of epileptic rats, so as to remedy their unbalance conditions. The effects of the medium and high dosage groups are more significant than the low; the21-day groups are a little more significant than the10-day ones.
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