力竭游泳运动后大鼠伏核NF-κB、cAMP表达的变化
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摘要
目的:观察力竭游泳运动后大鼠伏核NF-κB和cAMP的表达变化,探讨力竭运动后大脑伏核的调节机制,从而为指导运动训练、临床疾病的预防和运动康复治疗提供理论依据。
方法:选取80只SD大鼠,于适应性训练后随机分为安静对照组(n=10)和力竭组(n=70)。力竭组进行一周力竭性游泳训练后,按0h,4h,8h,12h,16h,20h,24h时间点,分别按冰水浴分离新鲜脑组织或多聚甲醛固定取脑的程序进行,前者经Western Blot和ELISA反应,分别检测伏核内NF-κB和环磷酸腺苷(cAMP)的含量变化,后者经免疫组织化学染色,观察NF-κB在伏核内的表达变化。对照组同时进行。
结果:(1)Western Blot显示,对照组大鼠伏核内NF-κB表达较少,力竭运动后NF-κB表达明显增多。力竭运动后,8h时达到较高水平,以后维持在较高水平,于16h时达到高峰,然后才开始回落。(2)力竭运动后,0 h时阳性神经元数目与对照组无统计学意义,4h后NF-κB阳性神经元数目均显著高于对照组, 8h达到较高水平,并维持在较高水平,持续至16h达到峰值后才开始下降。(3)对照组cAMP表达较低,力竭运动后在0h时,有一过性的升高,然后有所下降,但于8h又达到高峰,以后一直维持在较高水平,至16h后才开始缓慢下降。(4)力竭运动后, NF-κB和cAMP具有明显相同的时效性关系。
结论:(1)一周力竭游泳训练可以使大鼠大脑伏核中NF-κB与cAMP得到有效的活化。(2)一周力竭性游泳训练大鼠大脑伏核中NF-κB与cAMP两者变化趋势基本一致,提示它们可能具有协同效应。(3)NF-κB和cAMP信号通路可能作为一些与伏核相关的疾病的治疗手段,以及为指导运动训练提供理论依据。
Objective: To observe the expression of NF-κB and cAMP in Rat NAc of adult rats after exhausted swimming, and investigate the mechanism of NAc after exhausted swimming. Providing theoretical evidences for exercise training,and offering new ideas for exercise induced disease prevention and sport treatment.
Methods: Eighty SD rats were divided into the control group (n=10) and exhausted group (n=70) after adaptive training. The fresh cerebral tissues were divided on ice water bath while the paraform-perfused brains were obtained at 0h,4h,8h,12h,16h,20h,24h after exhausted swimming for a week. The former cerebral tissues were detected by Western Blot and ELISA in order to investigate the changes of NF-κB and cAMP in NAc, however, the later perfused brains be done with frozen section, immunohistochemical staining, then the change of expression of NF-κB were observed. The same to control group.
Results: (1). Western Blot showed, the expression of NF-κB was extremely lower in control group, but increased higher after exercise. In NAc, the expression of NF-κB increased to the higher level at 8h after exhausted swimming,then,retainned high level for a monment, peaked at 16h, then descended gradually. (2). Compared with control group, the number of NF-κB+ neurons had no significant difference at 0h. Several NF-κB+ neurons of control group distributed in the NAc dispersively while the exhausted groups expressed significantly higher after 4h, and increased to the higher level at 8h, then, retained the high level, peaked at 16h, then descended dramatically.(3). The expression of cAMP was extremely lower in control group, but transiently increased at 0h after exhausted exercise, then increased to the top at 8h ,retained high level to 16h,and descended gradually.(4). NF-κB is the same as cAMP in time course.
Conclusion: (1). NF-κB and cAMP can be activated effectively in NAc after exhausted exercise. (2).the tendencys of change of NF-κB and cAMP in NAc after exhausted exercise are similar, It is suggested that they have synergistic effect. (3). NF-κB and cAMP signals passways may be offering new therapy means for relative-NAc-disease,and providing theoretical evidences for exercise training.
方法:选取80只SD大鼠,于适应性训练后随机分为安静对照组(n=10)和力竭组(n=70)。力竭组进行一周力竭性游泳训练后,按0h,4h,8h,12h,16h,20h,24h时间点,分别按冰水浴分离新鲜脑组织或多聚甲醛固定取脑的程序进行,前者经Western Blot和ELISA反应,分别检测伏核内NF-κB和环磷酸腺苷(cAMP)的含量变化,后者经免疫组织化学染色,观察NF-κB在伏核内的表达变化。对照组同时进行。
结果:(1)Western Blot显示,对照组大鼠伏核内NF-κB表达较少,力竭运动后NF-κB表达明显增多。力竭运动后,8h时达到较高水平,以后维持在较高水平,于16h时达到高峰,然后才开始回落。(2)力竭运动后,0 h时阳性神经元数目与对照组无统计学意义,4h后NF-κB阳性神经元数目均显著高于对照组, 8h达到较高水平,并维持在较高水平,持续至16h达到峰值后才开始下降。(3)对照组cAMP表达较低,力竭运动后在0h时,有一过性的升高,然后有所下降,但于8h又达到高峰,以后一直维持在较高水平,至16h后才开始缓慢下降。(4)力竭运动后, NF-κB和cAMP具有明显相同的时效性关系。
结论:(1)一周力竭游泳训练可以使大鼠大脑伏核中NF-κB与cAMP得到有效的活化。(2)一周力竭性游泳训练大鼠大脑伏核中NF-κB与cAMP两者变化趋势基本一致,提示它们可能具有协同效应。(3)NF-κB和cAMP信号通路可能作为一些与伏核相关的疾病的治疗手段,以及为指导运动训练提供理论依据。
Objective: To observe the expression of NF-κB and cAMP in Rat NAc of adult rats after exhausted swimming, and investigate the mechanism of NAc after exhausted swimming. Providing theoretical evidences for exercise training,and offering new ideas for exercise induced disease prevention and sport treatment.
Methods: Eighty SD rats were divided into the control group (n=10) and exhausted group (n=70) after adaptive training. The fresh cerebral tissues were divided on ice water bath while the paraform-perfused brains were obtained at 0h,4h,8h,12h,16h,20h,24h after exhausted swimming for a week. The former cerebral tissues were detected by Western Blot and ELISA in order to investigate the changes of NF-κB and cAMP in NAc, however, the later perfused brains be done with frozen section, immunohistochemical staining, then the change of expression of NF-κB were observed. The same to control group.
Results: (1). Western Blot showed, the expression of NF-κB was extremely lower in control group, but increased higher after exercise. In NAc, the expression of NF-κB increased to the higher level at 8h after exhausted swimming,then,retainned high level for a monment, peaked at 16h, then descended gradually. (2). Compared with control group, the number of NF-κB+ neurons had no significant difference at 0h. Several NF-κB+ neurons of control group distributed in the NAc dispersively while the exhausted groups expressed significantly higher after 4h, and increased to the higher level at 8h, then, retained the high level, peaked at 16h, then descended dramatically.(3). The expression of cAMP was extremely lower in control group, but transiently increased at 0h after exhausted exercise, then increased to the top at 8h ,retained high level to 16h,and descended gradually.(4). NF-κB is the same as cAMP in time course.
Conclusion: (1). NF-κB and cAMP can be activated effectively in NAc after exhausted exercise. (2).the tendencys of change of NF-κB and cAMP in NAc after exhausted exercise are similar, It is suggested that they have synergistic effect. (3). NF-κB and cAMP signals passways may be offering new therapy means for relative-NAc-disease,and providing theoretical evidences for exercise training.
引文
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