鸭疫里氏杆菌免疫诊断方法及在感染鸭肝脏差异表达基因的研究
|
摘要
鸭疫里氏杆菌(Riemerella anantipestifer,RA)病是由鸭疫里氏杆菌引起的雏鸭的一种急性、接触性败血性传染病,是严重危害我国养鸭业的重要传染病之一。鸭疫里氏杆菌血清型复杂,各血清型之间缺乏有效的交叉免疫保护,这给使用全菌灭活疫苗或弱毒疫苗进行该病的免疫防治带来了诸多困难。临床分离菌株的鉴定和血清定型及鸭群鸭疫里氏杆菌抗体的监测,对了解鸭疫里氏杆菌的流行情况和制定有效的免疫计划以及评价疫苗免疫效果具有重要意义。鸭疫里氏杆菌在入侵宿主后,迅速在感染位点定居、繁殖,经血液扩散到全身各组织器官,引起全身多发性浆膜炎并发展为败血症。而目前对鸭疫里氏杆菌如何在宿主体内生存、繁殖和逃避免疫系统监视并致病的分子机制知之甚少。本研究针对我国流行的血清1型鸭疫里氏杆菌的42kD外膜蛋白OmpA进行了克隆、表达和免疫原性研究,探索外膜蛋白OmpA的免疫保护作用及其作为包被抗原建立用于鸭疫里氏杆菌分子流行病学调查的ELISA诊断方法;在此基础上,利用选择性捕获转录序列技术对鸭疫里氏杆菌在感染鸭肝脏基因差异表达进行了研究,为进一步开展了鸭疫里氏杆菌致病分子机理的研究,阐明雏鸭腹腔广泛性浆液渗出的分子机制奠定基础。本研究取得的结果如下:
1.鸭疫里氏杆菌平板染色诊断抗原的制备和应用
用本实验室鉴定、保存的血清1型鸭疫里氏杆菌云梦分离株(RA-YM)制备了RA平板染色诊断抗原,该抗原与鸭流感、鸭瘟、鸭肝炎、鸭大肠杆菌和鸭沙门氏菌的等5种病原的阳性血清无交叉反应;染色抗原平板凝集试验与琼脂免疫扩散试验(AGID)对RA疫苗免疫鸭的抗体检出率结果表明,在疫苗免疫后12天,平板凝集法即可检测到RA抗体,检出率为30%,AGID在免疫后31天才能检测到抗体,检出率为10%,而平板凝集法的检出率为80%,平板凝集法抗体检出率明显高于AGID,且染色抗原平板凝集试验比AGID的灵敏度高3个滴度;不同批次的平板染色诊断抗原检测的阳性率基本相当,在凝集程度上有较小的差异,表明不同批次平板染色诊断抗原具有良好的重复性。以RA-YM和纯化的鸭IgG为免疫原免疫鸭和兔,制备了鸭抗1型RA和兔抗鸭IgG的高免血清,平板凝集试验检测鸭抗RA-YM高免血清的效价为1:32,兔抗鸭IgG高免血清的琼扩效价为1:64。以制备的鸭抗血清1型RA血清对分离自湖北省不同地区的23株鸭疫里氏杆菌进行血清型鉴定,结果均为血清1型,表明我省目前流行的RA主要是血清1型。将制备的兔抗鸭IgG高免血清纯化后,经辣根过氧化物酶(HRP)标记,制备出特异性强、免疫学活性高的兔抗鸭IgG-HRP,工作浓度为1:4000,为下一步建立一种用于RA流行病调查的间接ELISA诊断方法奠定了基础。
2.鸭疫里氏杆菌OmpA基因的克隆、表达及重组OmpA蛋白免疫保护性试验研究
根据RA15型CVL110/89株OmpA基因序列(GeneBank登录号:AF104936)合成特异性引物,PCR扩增了RA-YM株OmpA基因,克隆到pMDl8-T,获得重组质粒pT-OmpA并进行了测序;将OmpA克隆到pGEX-KG,构建了重组原核表达质粒pGEX-OmpA,转化E.coli BL21后经IPTG诱导获得高效表达,经SDS-PAGE分析,表达的重组蛋白的分子量为68kD,表达产物主要以包涵体形式存在,Western-blot证实表达产物具有良好的生物学活性。将重组表达的OmpA蛋白以0.5mg、1.0mg、1.5mg三个免疫剂量分别与弗氏完全佐剂乳化后免疫7日龄樱桃谷肉鸭,17日龄以同样剂量加强免疫,二免后8天攻毒(3×10~6CFU/只),在攻毒24h后蛋白免疫组和未免疫空白组均发病,动物试验结果表明重组表达的OmpA不能起到保护作用。
3.鸭疫里氏杆菌间接OmpA-ELISA检测方法的建立及初步应用
用大肠杆菌表达的重组蛋白OmpA建立了RA抗体OmpA-ELISA检测方法,方阵滴定确定了抗原最佳包被浓度为0.81μg/mL,血清最佳稀释倍数为1:160。通过检测84份鸭疫里氏杆菌病阴性血清,确定该方法的阴阳性临界值为0.22。用该方法检测鸭流感,鸭瘟,鸭肝炎,鸭大肠杆菌和鸭沙门氏菌的标准阳性血清OD_(450)值均小于临界值0.22,表明该方法与其它病原抗体无交叉反应,特异性强。重复性试验表明OmpA-ELISA检测方法的批内重复和批间重复的变异系数均小于10%。用建立的OmpA-ELISA检测方法和平板凝集分别对213份送检血清进行检测,结果表明OmpA-ELISA检测方法的敏感性为95.77%,二者的符合率为62.91%。将湖北汉川、荆州和武汉市江夏区、黄陂区等地送检的472份鸭血清采用建立的OmpA-ELISA检测方法进行检测,结果表明RA抗体阳性率为64.6%,说明建立的间接OmpA-ELISA检测方法能够很好的应用于临床RA抗体检测。
4.应用选择性捕获转录序列技术(SCOTS)筛选鸭疫里氏杆菌在感染鸭肝脏中差异表达的基因的研究
根据RAD-24105株16S rRNA基因序列(GeneBank登录号:AY871819),合成特异性引物,PCR扩增了RA-YM株16S rRNA基因,克隆到pMD18-T,获得重组质粒pT-16SrDNA并进行测序,结果表明克隆的RA-YM株16S rRNA为1479bp,其GeneBank登录号为FJ031240。比对分析Genbank中已公布的表皮葡萄球菌(GeneBank登录号:NC_002737),多杀巴氏杆菌(GeneBank登录号:NC_002663)、大肠杆菌(GeneBank登录号:NC_000913)和噬二氧化碳单胞菌(GeneBank登录号:AY661855)23S rRNA基因序列的保守区域,利用Primer premier 5.0设计3对引物,分3段分别扩增了23S rRNA基因,克隆到pMD18-T,获得重组质粒pT-23S2、pT-23S1和pT-23S3并进行测序,拼接结果表明克隆的RA-YM株23S rRNA为2655bp,其GeneBank登录号为FJ031241。对生物素标记的RA-YM基因组DNA和构建好的RA-YM核糖体rDNA质粒(pT-16SrDNA,pT-23S-1rDNA和pT-23S-3rDNA)超声破碎,凝胶电泳结果表明破碎产物大小为100-2000bp。
以血清1型RA-YM感染9日龄樱桃谷肉鸭,提取感染鸭肝脏的总RNA和TSB培养基中生长的RA-YM的总RNA,经SuperScript~(TM)ⅢRTase反转录,Klenow酶合成cDNA第二链后,记为体内感染cDNAs(in vivo cDNAs)和RA-YM体外培养cDNAs(in vitro cDNAs)。对in vivo cDNAs和in vitro cDNAs进行3轮SCOTS,得到均一化的RA-YM在体内感染时转录的cDNAs和在TSB培养时转录的cDNAs,通过3轮差异杂交选择性捕获RA-YM在感染鸭肝脏中差异表达的基因,将RA-YM在感染鸭肝脏中差异基因的PCR产物克隆到pMD18-T载体,转化E.coli DH5α感受态细胞,通过PCR和斑点杂交,从文库中筛选出187个差异克隆,经序列测定和比对分析获得了48个RA-YM在感染鸭肝脏中差异表达的基因。Real-time RT-PCR对甘氨酸裂解酶T亚基,尿黑酸1,2-加氧酶,天冬氨酸转氨酶,触酶,磷酸盐转运蛋白,二肽肽酶Ⅳ,肽酶M28,和RA46等8个基因的差异表达情况进行验证,结果表明与生长于TSB培养基RA的基因表达水平相比,这8个基因在RA感染过程中的表达水平上调1.44-4.62倍,t-检验分析表明8个基因的表达水平差异显著。根据基因功能,将48个基因分为六类:合成与代谢,适应性调节,应激,转运系统,蛋白酶和5个未知功能序列。本研究初步揭示了RA在感染鸭肝脏的基因表达情况,获得的差异表达基因对研究RA在宿主体内的存活、增殖及持续感染具有重要的作用。
本研究筛选到二肽肽酶Ⅳ(Dipeptidyl peptidaseⅣ,DPPⅣ)可能是RA重要的毒力因子。DPPⅣ是一种丝氨酸蛋白酶,能特异性水解氨基端第二位是脯氨酸的多肽。牙龈卟啉单胞菌的DPPⅣ能够降解结缔组织以及介导牙龈卟啉单胞菌与纤粘蛋白的粘附,缺失dppⅣ基因的牙龈卟啉单胞菌的毒力明显降低。格氏链球菌的DPPⅣ可以酶解P物质,协同胞外精氨酸蛋白酶酶解缓激肽,导致血管通透性的变化及感染的内皮组织平滑肌的收缩。雏鸭感染鸭疫里氏杆菌后,主要病理变化是全身各组织器官的浆膜面出现广泛性的纤维素性渗出,RA感染严重损伤了雏鸭的血管内皮系统,导致血管通透性的增高。因此,二肽肽酶Ⅳ可能是RA关键的毒力因子。
下一步研究将克隆、表达和缺失RA的dppⅣ基因,以及利用酵母双杂交系统筛选和鉴定与DPPⅣ互作的宿主蛋白,为进一步研究dppⅣ对RA的毒力以及RA的致病机理奠定基础。
The Riemerella anatipestifer infection is one of the most important diseases to duck industry throughout the world,which usual occurs as an acute,contagious,septic disease in ducks.Infected ducks have fibrinous pericarditis,perihepatitis,meningitis and peritonitis.R.anatipestifer causes increased mortalities,decreased weight gains,and increased condemnations at slaughter and is estimated to cause substantial economic losses to the commercial duck industry each years.R.anatipestifer have complex serotypes,among which lack of effective cross-protection,leading to the difficulty of using inactivated bacterins,live or cell-free filtrates vaccines to immunize ducks against infection.Characterizaton of the field isolates and differentiation of theirs serotype, respectively,and monitoring of antibodies against R.anatipestifer,which play a very important role in understanding epidemic status of R.anatipestifer and formulating immunized procedure and evaluating immunized effect.R.anatipestifer firstly causes local inflammation at the site of entry,then rapidly break through the defensive organizations into blood.After the proliferation in blood,R.anatipestifer located mainly in serous membrane all over the body,cause inflammation in here and result in septicemia. Howerer,at present,little is known about the survive and multiply while avoiding the host immune system within a host organism of R.anatipestifer during infection.Therefore,we explored that development of OmpA-ELISA detecting antibodies against R.anatipestifer and differential expressed genes of R.anatipestifer in infected duck livers by selective capture of transcribed sequence technique.The research contents are summarized as follows:
1.Preparation and preliminary of stain plate agglutination disgnostic antigen of R. anatipestifer
The serotype 1 field isolate,R.anatipestifer RA-YM was used to prepare diagosis antigen of stain plate agglutination.The results showed that has a good specificity, sensitivity and repeatability.It has no cross-reaction with five cluck diseases sera,and titer dectected by stain plate agglutination is higher than agar immune diffusion.This work showed stain plate agglutination could be used for antibodies detection and diagnosis for R.anatipestifer,which will play an active role in prevention and control the R. anatipestifer disease.Ducks were immunized with serovar 1,R.anatipestifer RA-YM emusified with Frund's adjuvant,and sera of duck against RA-YM were acquired after four inoculations,and the titers of plate agglution reach 1:32.Twenty-three R. anatipestifer isolated from Hubei province were identificated by antisera prepared above, the result showed thet are serovar 1.At the same time,purified serum of rabbit anti-duck IgG(AGID:1:64) from rabbit immunized with purified duck IgG was labeled with horseradish peroxidase(HRP).The conjugate is characteristic of high activity of immunity and its work concentration reached 1:4000.This laid a foundation for the development of indirect enzyme-linked immunosorbent assay(ELISA) for R. anatipestifer.
2.Cloning,expression of the OmpA gene of Riemerella anatipestifer and protection test of rOmpA in ducks
The OmpA gene of RA-YM was amplified by PCR technique and cloned into pMD 18-T,and recombinant plasmid of pT-OmpA was sequenced by Sanger's sequencing techqique.Then,the OmpA from pT-OmpA was inserted into the pGEX-KG vector,to yield the recombinant plasmid pGEX-OmpA.After induced by IPTG,a high expression of fusion proteins was obtained.SDS-PAGE analysis showed that the fusion proteins was 68 kD in size.rOmpA existed mainly in form of inclusion bodies and was specific to antisera against R.anatipestifer by western blot analysis.The rOmpA was used to immunize ducks,the result showed rOmpA couldn't protect vaccinated ducks from challenge with RA-YM.
3.Development and preliminary of OmpA-ELISA diagnostic method
The OmpA-ELISA was developed using expressed rOmpA in E.coli.The suitable concentration of coated antigen,0.81μg/mL,and the serum dilutions,1:160,were selected by checkerboard.The cut-off value determined by testing 84 negative sera was 0.22.In the analysis of indirect ELISA cross specificity,it revealed a negative reaction with positive sera of duck influenza,duck hepatitis,duck plague,s.anatum,E.coli(O_(78)),serum of duck uninfected with R.anatipestifer and a positive reaction with standard positive serum of or R.anatipestifer.In order to know its reliability,a repetitive experiment was conducted in different ELISA in the same time and in the different time that result in less than 10%variable coefficient of absoprotion.To evaluate the specificity and sensitivity of the OmpA-ELISA,213 field sera samples were collected from chickens.Using flat plate agglutination test as the reference methods,the sensitivity of the OmpA-ELISA was 95.77%and coincidence 62.91%.Then a total of 472 duck sera samples were axamined that were sent from several field farms such as Hubei e.t.The results above indicated the assay to be a good method with strong specificity,high sensitivity and excellent coincidence and it could be used to fastly,accurately detect the antibodies against R. anatipestifer in the field sera samples.
4.Identification of Riemerella Anatipestifer Genes Differentially Expressed in Infected Duck Livers by Selective Capture of Transcribed Sequences Technique
The published sequences for R.anatipestifer 16S rDNA(AY871819) were used to design primers for the amplification of 16S rDNA of R.anatipestifer RA-YM.Because the 23S rDNA sequence of R.anatipestifer is not available,the 23S rDNA sequences of S. pyogenes(NC_002737),P.multocida(NC_002663),E.coli(NC_000913) and C. canimorsus strain 24231(AY661855) were aligned to identify the conservative regions for designing three sets of primers for the amplification of 23S rDNA of R.anatipestifer RA-YM.The primers were designed by using Primer premier 5.0.The amplified DNA fragments were then ligated into pMD 18-T vector and confirmed by sequencing analysis. The confirmed plasmids(pMD-16rRNA,pMD-23S1rRNA,and pMD-23S3rRNA) and biotinylated R.anatipestifer RA-YM genomic DNA were sonicated microprobe.The fragment of sample were 100-2000bp after soniacation.The sequence data of R. anatipestifer RA-YM rDNA are accessible with GenBank accession numbers:FJ031240 and FJ031241.
The ducks(n=7,9-days old) were experimental infected with R.anatipestifer RA-YM and 24 hours later,the liver samples from these dusks were collected and immediately put into liquid nitrogen.Total RNA from R.anatipestifer RA-YM infected livers or from TSB controls was isolated,and double cDNAs were synthesized,which were named by in vivo and in vitro cDNAs,respectively.After three rounds of normalization,the cDNAs from infected liver-grown R.anatipestifer were enriched and cloned into pMD18-T vectors to generate R.anatipestifer RA-YM-infected liver-specific cDNA libraries. Individual SCOTS clones obtained from liver-specific cDNA libraries were verified by PCR with SCOTS01 primers,and the PCR products of these SCOTS clones were spotted on positively charged membranes in duplicate subjected to dot blot analysis.The positive clones obtained from the dot blot analysis were identified and then sequenced by Sanger's sequencing techqique.BLASTn was performed to identify sequences of genes,and Swiss-Prot database was performed to identify sequences of translated products,there were up to 48 genes that were differentially expressed in the duck livers after R. anatipestifer infection compared to the control cultures.After that,eight genes(gcvT, hmgA,aspC,cat,ptfp,dppⅣ,m28,and RA46) were random chosen and verified by real-time RT-PCR analysis.All were upregulated by R.anatipestifer in infected duck livers,with changes ranging from 1.44- to 4.62- fold compared to in vitro cultures. Totally 48 genes were identified by SCOTS analysis,which can be divided into five functional groups:metabolism,regulatory,stress,transporter and proteinase.In our study, five genes of R.anatipestifer RA-YM encode different proteinase were identified,which have the highly homology with other bacteria.DPP IV is a serine protease that cleaves X-Pro or X-Ala dipeptide from the N-terminal ends of polypeptide chains.It is indicated that DPPIV is a important virulence factor of P.gingivalis by contributing to the degradation of connective tissues,and also mediates the adhesion of P.gingivalis to fibronectin.The proteolysis of substance P by Sg-xPDPP was observed,and the concerted action of an extracellular Arg aminopeptidase and Sg-xPDPP produces a truncated form of bradykinin.The combined effect of these modifications may result in local changes in vascular permeability and smooth muscle contraction at the infected endothelium.The fibrnous exudate is the prominent pathological characterization of R.anatipestifer infection,so the DPPⅣmay serve as a critical virulence factor of this organism for pathogenicity.
Inactivation of the DPPⅣgene in R.anatipestifer RA-YM using genetic tools should make it possible to determine the contribution of this protease to bacterial growth and pathogenicity.In the future,studies will focus on the isolation,expression and knockout of this gene or more of the in vivo expressed genes in order to evaluate their relative contributions to R.anatipestifer RA-YM virulence and survival at the site of infection.
1.鸭疫里氏杆菌平板染色诊断抗原的制备和应用
用本实验室鉴定、保存的血清1型鸭疫里氏杆菌云梦分离株(RA-YM)制备了RA平板染色诊断抗原,该抗原与鸭流感、鸭瘟、鸭肝炎、鸭大肠杆菌和鸭沙门氏菌的等5种病原的阳性血清无交叉反应;染色抗原平板凝集试验与琼脂免疫扩散试验(AGID)对RA疫苗免疫鸭的抗体检出率结果表明,在疫苗免疫后12天,平板凝集法即可检测到RA抗体,检出率为30%,AGID在免疫后31天才能检测到抗体,检出率为10%,而平板凝集法的检出率为80%,平板凝集法抗体检出率明显高于AGID,且染色抗原平板凝集试验比AGID的灵敏度高3个滴度;不同批次的平板染色诊断抗原检测的阳性率基本相当,在凝集程度上有较小的差异,表明不同批次平板染色诊断抗原具有良好的重复性。以RA-YM和纯化的鸭IgG为免疫原免疫鸭和兔,制备了鸭抗1型RA和兔抗鸭IgG的高免血清,平板凝集试验检测鸭抗RA-YM高免血清的效价为1:32,兔抗鸭IgG高免血清的琼扩效价为1:64。以制备的鸭抗血清1型RA血清对分离自湖北省不同地区的23株鸭疫里氏杆菌进行血清型鉴定,结果均为血清1型,表明我省目前流行的RA主要是血清1型。将制备的兔抗鸭IgG高免血清纯化后,经辣根过氧化物酶(HRP)标记,制备出特异性强、免疫学活性高的兔抗鸭IgG-HRP,工作浓度为1:4000,为下一步建立一种用于RA流行病调查的间接ELISA诊断方法奠定了基础。
2.鸭疫里氏杆菌OmpA基因的克隆、表达及重组OmpA蛋白免疫保护性试验研究
根据RA15型CVL110/89株OmpA基因序列(GeneBank登录号:AF104936)合成特异性引物,PCR扩增了RA-YM株OmpA基因,克隆到pMDl8-T,获得重组质粒pT-OmpA并进行了测序;将OmpA克隆到pGEX-KG,构建了重组原核表达质粒pGEX-OmpA,转化E.coli BL21后经IPTG诱导获得高效表达,经SDS-PAGE分析,表达的重组蛋白的分子量为68kD,表达产物主要以包涵体形式存在,Western-blot证实表达产物具有良好的生物学活性。将重组表达的OmpA蛋白以0.5mg、1.0mg、1.5mg三个免疫剂量分别与弗氏完全佐剂乳化后免疫7日龄樱桃谷肉鸭,17日龄以同样剂量加强免疫,二免后8天攻毒(3×10~6CFU/只),在攻毒24h后蛋白免疫组和未免疫空白组均发病,动物试验结果表明重组表达的OmpA不能起到保护作用。
3.鸭疫里氏杆菌间接OmpA-ELISA检测方法的建立及初步应用
用大肠杆菌表达的重组蛋白OmpA建立了RA抗体OmpA-ELISA检测方法,方阵滴定确定了抗原最佳包被浓度为0.81μg/mL,血清最佳稀释倍数为1:160。通过检测84份鸭疫里氏杆菌病阴性血清,确定该方法的阴阳性临界值为0.22。用该方法检测鸭流感,鸭瘟,鸭肝炎,鸭大肠杆菌和鸭沙门氏菌的标准阳性血清OD_(450)值均小于临界值0.22,表明该方法与其它病原抗体无交叉反应,特异性强。重复性试验表明OmpA-ELISA检测方法的批内重复和批间重复的变异系数均小于10%。用建立的OmpA-ELISA检测方法和平板凝集分别对213份送检血清进行检测,结果表明OmpA-ELISA检测方法的敏感性为95.77%,二者的符合率为62.91%。将湖北汉川、荆州和武汉市江夏区、黄陂区等地送检的472份鸭血清采用建立的OmpA-ELISA检测方法进行检测,结果表明RA抗体阳性率为64.6%,说明建立的间接OmpA-ELISA检测方法能够很好的应用于临床RA抗体检测。
4.应用选择性捕获转录序列技术(SCOTS)筛选鸭疫里氏杆菌在感染鸭肝脏中差异表达的基因的研究
根据RAD-24105株16S rRNA基因序列(GeneBank登录号:AY871819),合成特异性引物,PCR扩增了RA-YM株16S rRNA基因,克隆到pMD18-T,获得重组质粒pT-16SrDNA并进行测序,结果表明克隆的RA-YM株16S rRNA为1479bp,其GeneBank登录号为FJ031240。比对分析Genbank中已公布的表皮葡萄球菌(GeneBank登录号:NC_002737),多杀巴氏杆菌(GeneBank登录号:NC_002663)、大肠杆菌(GeneBank登录号:NC_000913)和噬二氧化碳单胞菌(GeneBank登录号:AY661855)23S rRNA基因序列的保守区域,利用Primer premier 5.0设计3对引物,分3段分别扩增了23S rRNA基因,克隆到pMD18-T,获得重组质粒pT-23S2、pT-23S1和pT-23S3并进行测序,拼接结果表明克隆的RA-YM株23S rRNA为2655bp,其GeneBank登录号为FJ031241。对生物素标记的RA-YM基因组DNA和构建好的RA-YM核糖体rDNA质粒(pT-16SrDNA,pT-23S-1rDNA和pT-23S-3rDNA)超声破碎,凝胶电泳结果表明破碎产物大小为100-2000bp。
以血清1型RA-YM感染9日龄樱桃谷肉鸭,提取感染鸭肝脏的总RNA和TSB培养基中生长的RA-YM的总RNA,经SuperScript~(TM)ⅢRTase反转录,Klenow酶合成cDNA第二链后,记为体内感染cDNAs(in vivo cDNAs)和RA-YM体外培养cDNAs(in vitro cDNAs)。对in vivo cDNAs和in vitro cDNAs进行3轮SCOTS,得到均一化的RA-YM在体内感染时转录的cDNAs和在TSB培养时转录的cDNAs,通过3轮差异杂交选择性捕获RA-YM在感染鸭肝脏中差异表达的基因,将RA-YM在感染鸭肝脏中差异基因的PCR产物克隆到pMD18-T载体,转化E.coli DH5α感受态细胞,通过PCR和斑点杂交,从文库中筛选出187个差异克隆,经序列测定和比对分析获得了48个RA-YM在感染鸭肝脏中差异表达的基因。Real-time RT-PCR对甘氨酸裂解酶T亚基,尿黑酸1,2-加氧酶,天冬氨酸转氨酶,触酶,磷酸盐转运蛋白,二肽肽酶Ⅳ,肽酶M28,和RA46等8个基因的差异表达情况进行验证,结果表明与生长于TSB培养基RA的基因表达水平相比,这8个基因在RA感染过程中的表达水平上调1.44-4.62倍,t-检验分析表明8个基因的表达水平差异显著。根据基因功能,将48个基因分为六类:合成与代谢,适应性调节,应激,转运系统,蛋白酶和5个未知功能序列。本研究初步揭示了RA在感染鸭肝脏的基因表达情况,获得的差异表达基因对研究RA在宿主体内的存活、增殖及持续感染具有重要的作用。
本研究筛选到二肽肽酶Ⅳ(Dipeptidyl peptidaseⅣ,DPPⅣ)可能是RA重要的毒力因子。DPPⅣ是一种丝氨酸蛋白酶,能特异性水解氨基端第二位是脯氨酸的多肽。牙龈卟啉单胞菌的DPPⅣ能够降解结缔组织以及介导牙龈卟啉单胞菌与纤粘蛋白的粘附,缺失dppⅣ基因的牙龈卟啉单胞菌的毒力明显降低。格氏链球菌的DPPⅣ可以酶解P物质,协同胞外精氨酸蛋白酶酶解缓激肽,导致血管通透性的变化及感染的内皮组织平滑肌的收缩。雏鸭感染鸭疫里氏杆菌后,主要病理变化是全身各组织器官的浆膜面出现广泛性的纤维素性渗出,RA感染严重损伤了雏鸭的血管内皮系统,导致血管通透性的增高。因此,二肽肽酶Ⅳ可能是RA关键的毒力因子。
下一步研究将克隆、表达和缺失RA的dppⅣ基因,以及利用酵母双杂交系统筛选和鉴定与DPPⅣ互作的宿主蛋白,为进一步研究dppⅣ对RA的毒力以及RA的致病机理奠定基础。
The Riemerella anatipestifer infection is one of the most important diseases to duck industry throughout the world,which usual occurs as an acute,contagious,septic disease in ducks.Infected ducks have fibrinous pericarditis,perihepatitis,meningitis and peritonitis.R.anatipestifer causes increased mortalities,decreased weight gains,and increased condemnations at slaughter and is estimated to cause substantial economic losses to the commercial duck industry each years.R.anatipestifer have complex serotypes,among which lack of effective cross-protection,leading to the difficulty of using inactivated bacterins,live or cell-free filtrates vaccines to immunize ducks against infection.Characterizaton of the field isolates and differentiation of theirs serotype, respectively,and monitoring of antibodies against R.anatipestifer,which play a very important role in understanding epidemic status of R.anatipestifer and formulating immunized procedure and evaluating immunized effect.R.anatipestifer firstly causes local inflammation at the site of entry,then rapidly break through the defensive organizations into blood.After the proliferation in blood,R.anatipestifer located mainly in serous membrane all over the body,cause inflammation in here and result in septicemia. Howerer,at present,little is known about the survive and multiply while avoiding the host immune system within a host organism of R.anatipestifer during infection.Therefore,we explored that development of OmpA-ELISA detecting antibodies against R.anatipestifer and differential expressed genes of R.anatipestifer in infected duck livers by selective capture of transcribed sequence technique.The research contents are summarized as follows:
1.Preparation and preliminary of stain plate agglutination disgnostic antigen of R. anatipestifer
The serotype 1 field isolate,R.anatipestifer RA-YM was used to prepare diagosis antigen of stain plate agglutination.The results showed that has a good specificity, sensitivity and repeatability.It has no cross-reaction with five cluck diseases sera,and titer dectected by stain plate agglutination is higher than agar immune diffusion.This work showed stain plate agglutination could be used for antibodies detection and diagnosis for R.anatipestifer,which will play an active role in prevention and control the R. anatipestifer disease.Ducks were immunized with serovar 1,R.anatipestifer RA-YM emusified with Frund's adjuvant,and sera of duck against RA-YM were acquired after four inoculations,and the titers of plate agglution reach 1:32.Twenty-three R. anatipestifer isolated from Hubei province were identificated by antisera prepared above, the result showed thet are serovar 1.At the same time,purified serum of rabbit anti-duck IgG(AGID:1:64) from rabbit immunized with purified duck IgG was labeled with horseradish peroxidase(HRP).The conjugate is characteristic of high activity of immunity and its work concentration reached 1:4000.This laid a foundation for the development of indirect enzyme-linked immunosorbent assay(ELISA) for R. anatipestifer.
2.Cloning,expression of the OmpA gene of Riemerella anatipestifer and protection test of rOmpA in ducks
The OmpA gene of RA-YM was amplified by PCR technique and cloned into pMD 18-T,and recombinant plasmid of pT-OmpA was sequenced by Sanger's sequencing techqique.Then,the OmpA from pT-OmpA was inserted into the pGEX-KG vector,to yield the recombinant plasmid pGEX-OmpA.After induced by IPTG,a high expression of fusion proteins was obtained.SDS-PAGE analysis showed that the fusion proteins was 68 kD in size.rOmpA existed mainly in form of inclusion bodies and was specific to antisera against R.anatipestifer by western blot analysis.The rOmpA was used to immunize ducks,the result showed rOmpA couldn't protect vaccinated ducks from challenge with RA-YM.
3.Development and preliminary of OmpA-ELISA diagnostic method
The OmpA-ELISA was developed using expressed rOmpA in E.coli.The suitable concentration of coated antigen,0.81μg/mL,and the serum dilutions,1:160,were selected by checkerboard.The cut-off value determined by testing 84 negative sera was 0.22.In the analysis of indirect ELISA cross specificity,it revealed a negative reaction with positive sera of duck influenza,duck hepatitis,duck plague,s.anatum,E.coli(O_(78)),serum of duck uninfected with R.anatipestifer and a positive reaction with standard positive serum of or R.anatipestifer.In order to know its reliability,a repetitive experiment was conducted in different ELISA in the same time and in the different time that result in less than 10%variable coefficient of absoprotion.To evaluate the specificity and sensitivity of the OmpA-ELISA,213 field sera samples were collected from chickens.Using flat plate agglutination test as the reference methods,the sensitivity of the OmpA-ELISA was 95.77%and coincidence 62.91%.Then a total of 472 duck sera samples were axamined that were sent from several field farms such as Hubei e.t.The results above indicated the assay to be a good method with strong specificity,high sensitivity and excellent coincidence and it could be used to fastly,accurately detect the antibodies against R. anatipestifer in the field sera samples.
4.Identification of Riemerella Anatipestifer Genes Differentially Expressed in Infected Duck Livers by Selective Capture of Transcribed Sequences Technique
The published sequences for R.anatipestifer 16S rDNA(AY871819) were used to design primers for the amplification of 16S rDNA of R.anatipestifer RA-YM.Because the 23S rDNA sequence of R.anatipestifer is not available,the 23S rDNA sequences of S. pyogenes(NC_002737),P.multocida(NC_002663),E.coli(NC_000913) and C. canimorsus strain 24231(AY661855) were aligned to identify the conservative regions for designing three sets of primers for the amplification of 23S rDNA of R.anatipestifer RA-YM.The primers were designed by using Primer premier 5.0.The amplified DNA fragments were then ligated into pMD 18-T vector and confirmed by sequencing analysis. The confirmed plasmids(pMD-16rRNA,pMD-23S1rRNA,and pMD-23S3rRNA) and biotinylated R.anatipestifer RA-YM genomic DNA were sonicated microprobe.The fragment of sample were 100-2000bp after soniacation.The sequence data of R. anatipestifer RA-YM rDNA are accessible with GenBank accession numbers:FJ031240 and FJ031241.
The ducks(n=7,9-days old) were experimental infected with R.anatipestifer RA-YM and 24 hours later,the liver samples from these dusks were collected and immediately put into liquid nitrogen.Total RNA from R.anatipestifer RA-YM infected livers or from TSB controls was isolated,and double cDNAs were synthesized,which were named by in vivo and in vitro cDNAs,respectively.After three rounds of normalization,the cDNAs from infected liver-grown R.anatipestifer were enriched and cloned into pMD18-T vectors to generate R.anatipestifer RA-YM-infected liver-specific cDNA libraries. Individual SCOTS clones obtained from liver-specific cDNA libraries were verified by PCR with SCOTS01 primers,and the PCR products of these SCOTS clones were spotted on positively charged membranes in duplicate subjected to dot blot analysis.The positive clones obtained from the dot blot analysis were identified and then sequenced by Sanger's sequencing techqique.BLASTn was performed to identify sequences of genes,and Swiss-Prot database was performed to identify sequences of translated products,there were up to 48 genes that were differentially expressed in the duck livers after R. anatipestifer infection compared to the control cultures.After that,eight genes(gcvT, hmgA,aspC,cat,ptfp,dppⅣ,m28,and RA46) were random chosen and verified by real-time RT-PCR analysis.All were upregulated by R.anatipestifer in infected duck livers,with changes ranging from 1.44- to 4.62- fold compared to in vitro cultures. Totally 48 genes were identified by SCOTS analysis,which can be divided into five functional groups:metabolism,regulatory,stress,transporter and proteinase.In our study, five genes of R.anatipestifer RA-YM encode different proteinase were identified,which have the highly homology with other bacteria.DPP IV is a serine protease that cleaves X-Pro or X-Ala dipeptide from the N-terminal ends of polypeptide chains.It is indicated that DPPIV is a important virulence factor of P.gingivalis by contributing to the degradation of connective tissues,and also mediates the adhesion of P.gingivalis to fibronectin.The proteolysis of substance P by Sg-xPDPP was observed,and the concerted action of an extracellular Arg aminopeptidase and Sg-xPDPP produces a truncated form of bradykinin.The combined effect of these modifications may result in local changes in vascular permeability and smooth muscle contraction at the infected endothelium.The fibrnous exudate is the prominent pathological characterization of R.anatipestifer infection,so the DPPⅣmay serve as a critical virulence factor of this organism for pathogenicity.
Inactivation of the DPPⅣgene in R.anatipestifer RA-YM using genetic tools should make it possible to determine the contribution of this protease to bacterial growth and pathogenicity.In the future,studies will focus on the isolation,expression and knockout of this gene or more of the in vivo expressed genes in order to evaluate their relative contributions to R.anatipestifer RA-YM virulence and survival at the site of infection.
引文
1.[美]SaifY M主编;苏敬良,高福,索勋 主译.禽病学(第十一版)(世界兽医经典著作译丛).北京:中国农业出版社,2005
2.白洁,李雪飞,黄波,李莹,王波,吕申.DNA错配修复基因产物表达与肿瘤恶性程度相关性的初步探讨.大连医科大学学报,1998,20(4):24-25
3.鲍国连,佟承刚,韦强,季全安,费炳荣,陶锡荣,姜为民.鸭传染性浆膜炎流行病学与病原特性研究.浙江农业学报,1997,9(3):161-164
4.鲍国连,佟承刚,韦强,季全安,费炳荣,陶锡荣,姜为民.鸭传染性浆膜炎疫苗的研究.中国预防兽医学报,1999,21(6):414-416
5.毕利军,周亚凤,邓教宇,张先恩,张成刚.DNA错配修复系统研究进展.生物化学与生物物理进展,2003,30(1):32-37
6.蔡畅,曲丰发,郑献进,丁春宇,张大丙.鸭疫里默氏菌16S rDNA基因的单链构象多态性分析.中国兽医杂志,2006,42(4):10-12
7.蔡家利,唐晓丽,杨龙峰,苏晓运。鸭传染性浆膜炎蜂胶疫苗研制.中国兽医学报,2001,21(6):552-553
8.蔡家利,詹晓庆.鸭疫里氏杆菌和大肠杆菌二联灭活疫苗研究.中国家禽,2004,26(20):10-12
9.蔡秀磊,韦强,崔言顺,鲍国连,霍翠梅,季权安,李建亮,张先福.鸭疫里默氏杆菌临床分离株的耐药性与Ⅰ型整合子检测.中国预防兽医学报,2007,29(9):727-731
10.曹素芳,黄青云.禽多杀性巴氏杆菌C_(48-1),成熟外膜蛋白重组亚单位疫苗免疫效果的研究.中国兽医科学,2006,36(6):464-467
11.陈芬芬.鸭疫里氏杆菌体内诱导抗原基因的筛选与应用研究.[硕士学位论文].湖北武汉,华中农业大学,2008
12.陈师勇,莫照兰,张振东,邹玉霞,徐永立,张培军.细菌毒力基因体内表达检测技术研究进展.遗传,2005,27(3):505-511
13.陈兴生,李布勇.海南省鸭疫里默氏杆菌流行病学调查.中国预防兽医学报,1999,21(3):231-233
14.程安春,汪铭书,陈孝跃,钟妮娜,赖为民.鸭疫巴氏杆菌的分离和鉴定.四川畜牧兽医,1996,2:7-9
15.程安春,汪铭书,陈孝跃,朱德康,黄城,刘菲,周毅,郭宇飞,刘兆宇,方鹏飞.我国鸭疫里默菌血清型调查及新血清型的发现和病原特性.中国兽医学报,2003b,23:320-323
16.程安春,汪铭书,方鹏飞,郭宇飞,刘兆宇,陈孝跃,周毅.间接酶联免疫吸附试验(ELISA)检测血清4型鸭疫里默氏杆菌抗体的研究.中国家禽,2004b,16:14-17
17.程安春,汪铭书,方鹏飞,郭宇飞,刘兆字,陈孝跃,周毅.间接酶联免疫吸附试验((ELISA)检测血清2型鸭疫里默氏杆菌抗体的研究.中国兽医杂志,2004a,40:14-17
18.程安春,汪铭书,郭宇飞,方鹏飞,刘兆宇,陈孝跃,周毅.鸭疫里默氏菌铝胶佐剂与铝胶复合佐剂4价灭活疫苗的比较.中国兽医学报,2005,25(2):152-156
19.程安春,汪铭书,郭宇飞,朱德康,陈孝跃,刘维平,孟琼华,李淑梅.鸭疫里墨氏杆菌病及其在我国的流行动态和防治对策.中国家禽,2003a,22:29-31
20.程安春,汪铭书,刘兆宁,周毅,陈孝跃,方鹏飞,郭宇飞.血清2型鸭疫里默氏杆菌外膜蛋白多肤的组成及免疫原性.中国兽医学报,2004c,24:329-331
21.程龙飞,傅光华,施少华,陈红梅,彭春香,黄瑜,林芳,林建生.Ⅰ型鸭疫里氏杆菌基因组质粒表达文库的构建.江西农业大学学报,2007,29(6):887-890
22.程龙飞,施少华,李文杨,傅光华,彭春香,黄瑜.1、2型鸭疫里默氏菌外膜蛋白分析.福建农林大学学报(自然科学版),2005,34(2):260-262
23.程龙飞,施少华,傅光华,李文杨,彭春香,黄瑜,陈溥言.2型鸭疫里氏杆菌抗体间接ELISA检测方法的建立与应用.福建农业学报,2006,21(2):131-134
24.程龙飞,施少华,傅光华,彭春香,黄瑜,陈红梅,万春和.2型鸭疫里默氏菌抗体效价与免疫保护率的相关性测定.福建畜牧兽医,2009,31(2):25-26
25.程龙飞,施少华,李文杨,傅光华,黄瑜.PCR检测鸭疫里氏菌的研究.江西农业大学学报,2004,26(1):131-133
26.程龙飞,钟敏,郑腾,傅光华,陈红梅,施少华,白泉阳,黄瑜.鸭疫里氏杆菌敏感烈性噬菌体的分离鉴定.中国兽医科学,2009,39(02):106-109
27.邓舜洲,叶林方,何后军,朱芝秀,谢伟东.江西地区鸭疫里氏杆菌微生物学特性的研究.江西农业大学学报(自然科学版),2002,24:502-507
28.董传甫,林天龙,龚晖,欧阳岁东,杨苏.嗜水气单胞菌主要外膜蛋白对欧洲鳗鲡的免疫保护试验.水生生物学报,2005,29(3):285-290
29.杜念兴主编.兽医免疫学,第二版.北京:中国农业出版社,1997
30.范京惠,左玉柱,李潭清,宋勤叶,赵坤乾.鸭疫里默氏杆菌河北株的分离鉴定及药敏试验.养禽与禽病防治,2007,9,30-31
31.房宝志,孙晓燕,殷廷秀.微山县首次发现鸭传染性浆膜炎.山东畜牧兽医,2000,2:43
32.高福,郭玉璞,程锡麟.小鸭传染性浆膜炎疫苗的研究:II鸭疫巴氏杆菌灭活苗的初步研究.中国兽医杂志,1987,13(4):51-53
33.高福,郭玉璞,张应国.小鸭传染性浆膜炎疫苗的研究:Ⅲ鸭疫巴氏杆菌矿物油佐剂灭活苗.中国兽医杂志,1990,16:46-48
34.高福,郭玉璞.小鸭传染性浆膜炎疫苗的研究:Ⅰ鸭疫巴氏杆菌的血清型鉴定.中国兽医杂志,1987,13(4):47-48
35.高崧,吴晓东,张扬,刘秀梵,张如宽.禽大肠杆菌外膜蛋白、脂多糖疫苗的免疫保护试验.中国兽医学报,2002,22(5):457-459
36.顾宏伟,陆承平.兔多杀性巴氏杆菌铁调节外膜蛋白的小鼠免疫效果分析.中国农业科学,2007,40(5):1073-1078
37.郭春祥和郭锡琼.介绍一种简单、快速、高效的辣根过氧化物酶标记抗体的过碘酸钠法.上海免疫学杂志,1983,3(2):97-100
38.郭予强,杨连楷,凌育粲,詹乐群.鸭疫巴氏杆菌病及其并发感染的防治.养禽与禽病防治,1994,3:28-29
39.郭予强.鸭疫综合征的研究.华南农学院学报,1983,4(2):11-20
40.郭宇飞,程安春,汪铭书,方鹏飞,刘兆宇,陈孝跃,周毅.鸭疫里氏杆菌病四价灭活疫苗的研究.中国兽医科技,2003,33(6):3-9
41.郭玉璞,陈德威,陈俊杰.鸭疫巴氏杆菌和多杀巴氏杆菌在电子显微镜下的比较研究.北京农业大学学报,1986,12(1):261-270
42.郭玉璞,陈德威,范国雄,刘瑞萍.北京鸭鸭传染性浆膜炎的调查研究.畜牧兽医学报,1982,13(2):107-112
43.郭玉璞,甘孟侯,张中直.应用荧光抗体法诊断小鸭传染性英膜炎的试验.中国畜禽传染病,1998,20(3):183-186
44.何昭阳,胡桂学,王春风主编.动物免疫学实验技术.长春:吉林科学技术出版社,2002,59-61。
45.洪帮兴,江丽芳,张世英,司徒潮满,林奕芝,钟跃星,胡玉山.病原菌23S rRNA基因序列变异性分析.疾病控制杂志,2004,8(5):385-388
46.胡清海,李刚,郑明球,蔡宝祥.间接ELISA检测鸭疫里氏杆菌抗体的研究.中国畜禽传染病,1998,20(6):354-356
47.胡清海,李刚,郑明球,蔡宝祥.鸭疫里氏杆菌江苏株G+C含量同源性研究.南京农业大学学报,1998,21(4):87-91
48.胡清海,刘晓文,苗晋锋,张知良,丁铲,章耀良.一株鹅源鸭疫里氏杆菌与鸭源分离株的致病性比较.畜牧与兽医,2002,34(9):3-4
49.胡清海,刘晓文,赵世华,张知良,丁铲,苗晋锋,刘华雷,赵东伟.应用PCR技术检测鸭疫里氏杆菌的研究.中国预防兽医学报,2002,24(6):471-473
50.胡清海,张知良,黄建芳.野鸭鸭疫里氏杆菌的分离和鉴定.中国家禽,2001,23(7):10-11
51.胡清海,张知良,苗晋锋,刘岳龙,刘晓文,丁铲.江苏安徽两省鸭疫里默氏杆菌病的流行病学调查研究.中国兽医科技,2001,31(8):12-13
52.胡晓娜,朱瑞良,刘红珍,毕建敏.禽波氏杆菌外膜蛋白的提取及其免疫原性的检测.微生物学报,2007,47(4):714-717
53.胡薛英,谷长勤,程国富,李自力,周诗其,毕丁仁,赵雅心.人工感染鸭疫里氏杆菌雏鸭的病理学变化.中国兽医科技,2004,34(3).48-50
54.黄庆洲,郭定章,但麒麟,林明明,伍莉.川渝两地鸭疫里氏杆菌分离鉴定及药效试验.四川畜牧兽医,2002,29(1):19-20
55.黄淑坚,王政富,梁文冠,吴志新,龚中贵,张莹,梁家桥,陈燕萍.粤北地区鸭疫里默氏杆菌的分离鉴定及药敏试验.中国家禽,2007,29(24):53-54
56.黄瑜,李文扬,程龙飞,庄向生,苏敬良,吕艳丽,郭玉璞.福建省血清Ⅱ型鸭疫里氏杆菌病的诊治.中国预防兽医学报,1999,21(1):
57.黄瑜,林世棠.鸭疫巴氏杆菌病的研究进展及存在的问题.中国家禽,1994,5:34-35
58.黄瑜,苏敬良,李文杨,程龙飞,彭春香,施少华,郭玉璞.1型鸭疫里默氏菌3种不同佐剂灭活苗的比较.中国兽医学报,2002.22:561-563
59.柯岩,陈哲生.制备兔抗人IgG抗体的一种简便方法.微生物学通报.1997,24(5):315-317
60.黎德兵,崔恒敏,章蕾.鸭疫里默氏杆菌病发病机理的研究:Ⅰ人工感染雏鸭部分血液指标的动态变化.黑龙江畜牧兽医,2004a,1:8-10
61.黎德兵,崔恒敏,章蕾.鸭疫里默氏杆菌病发病机理的研究:Ⅱ人工感染雏鸭病理形态学变化.黑龙江畜牧兽医,2004b,2:10-12
62.黎德兵,崔恒敏,赵翠燕.鸭疫里默氏杆菌感染后雏鸭血清生化指标变化的研究.畜禽业,2007,223(11):26-27
63.黎满香,董伟,雷红宇,宁玲忠.鸭疫里默氏菌和大肠杆菌混合感染的诊断及防制.畜牧兽医杂志,2004,23(3):46-47
64.黎晓敏,郭丽敏,唐发书,王健.鸭传染性浆膜炎病鸭免疫器官的光镜及电镜观察.畜牧兽医学报,2004,35(6):702-704
65.李继祥,徐刚,王孝友,杜华茂,黄伟.重庆地区鸭传染性浆膜炎病原分离鉴定.中国预防兽医学报,2000,22(6):402-422
66.李家奎,郭定宗,程大池,杨世锦,刘庆龙.武汉地区鸭疫巴氏杆菌的分离鉴定.中国预防兽医学报,2000,22:423-424
67.李康然.鸭疫巴氏杆菌感染.广西畜牧兽医,1996,12(3):52-52
68.李霆,高志勇,王恒梁,冯尔玲,陈宗德,李新月,黄留玉,黄翠芬.应用体内诱导抗原技术筛选结核分枝杆菌体内表达基因.遗传学报,2005,32(2):111-117
69.李文杨,程龙飞,彭春香,黄瑜.福建省鸭疫里默氏菌血清流行病学调查.中国预防兽医学报,2002,24(2):138-139
70.李晓霞,邱玉玉,王海荣.肠致病性大肠杆菌外膜蛋白免疫保护性研究.中国免疫学杂志,2007,23(5):394-397
71.李自力,毕丁仁,许青荣,石德时,程峰,刘梅.鸭传染牲浆膜炎的诊断与防治.湖北畜
牧兽医,2003,4:34-35
72.李自力,熊涛,毕丁仁,石德时,胡薛英,许青荣,程峰.湖北鸭疫里氏杆菌分离株人工感染试验及灭活疫苗研制.湖北农业科学,2004,5:87-89
73.梁月丽,陶海静.鸭疫巴氏杆菌的分离与鉴定.河南畜牧兽医,2003,24:7-8
74.廖立新,曹郁生,李国辉,陈燕,张万山,熊勇华,姚闽.铜绿假单胞菌外膜蛋白的免疫原性及免疫保护性.中华微生物学和免疫学杂志,2004,24(7):571-572
75.林丽超,陈建红,黄盛,张济培,张元跃.鸭疫里氏杆菌疫苗在雏鸭体内诱导细胞免疫的动态研究.佛山科学技术学院学报(自然科学版),2005,23(3):73-76
76.林琳,吴南洋,江斌,吴胜会.儿种药物对鸭疫里氏杆菌的抑菌试验.福建畜牧兽医,2000,22(2):11-12
77.林世堂,郁晓岚,黄瑜.鸭疫巴氏杆菌亚单位成分的提取及免疫原性初步研究.福建畜
78.林禧龄,曾彦钦.福州首次发现鸭疫巴氏杆菌病.中国兽医杂志,1992,18(9):12-13
79.林业杰,林禧龄,曾颜钦.福州首次从康贝尔鸭分离出鸭疫巴氏杆菌.中国兽医科技,1989,2:26-27
80.刘维平,程安春,江铭书,陈孝跃,周毅,朱德康,刘菲,孟琼华,黄诚,宋涌.间接免疫组化法检测鸭疫里默氏杆菌感染和抗原定位.中国兽医学报,2005,25(4):362-365
81.刘文华,苏敬良,刘颖,许秀梅,金鑫,吴培福.16s rRNA基因及外膜蛋白基因分析在鸭疫里莫氏杆菌鉴定中的应刚.中国预防兽医学报,2006,28(6):691-696
82.刘小艳.氟苯尼考注射液治疗鸭疫里默氏杆菌病的疗效试验.吉林畜牧兽医,2004,6:51
83.刘颖,苏敬良,刘文华,李云格.鸭疫里默氏菌的药物敏感性检测.中国兽医杂志,2005,41(5):10-12
84.刘颖,苏敬良,刘文华.鸭疫里默氏菌的gyrA基因的检测与变异位点的分析.中国兽医杂志,2007,43(1):12-14
85.刘永德,吴祖立,叶陈梁,高莉莉.鸭疫巴氏杆菌与大肠杆菌二联苗研究.中国畜禽传染病,1997,93(2):4-6
86.陆承平.兽医微生物学第三版.中国农业出版社,2001,9
87.吕殿红,张毓金,干刚.鸭疫里氏杆菌病的研究进展.动物医学进展,2000,21(4):189-192
88.吕殿红.鸭疫里默氏杆菌菌种保存试验及蜂胶苗的保护试验.动物医学进展,2003,23(1):79-81
89.吕敏娜,黄承锋,张玉团,张健马非,杨海金.鸭传染性浆膜炎荚膜多糖苗研究初报.广东畜牧兽医科技,1999,3:16-18
90.吕敏娜,黄承锋,张毓金.鸭疫里默氏杆菌大肠杆菌二联蜂胶苗的研制.中国兽医杂志,2005,41:48-50
91.罗东生.我省首次发现小鸭传染性浆膜炎.湖南畜牧兽医,1997,6:31
92.毛娅卿,翁崇鹏,刘烈发,章振华,堪南辉.三肤囊素Bursin对鸭疫里氏杆菌病灭活苗免疫效果的影响.江西农业大学学报,2005,27:282-284
93.孟琼华,程安春,汪铭书,陈孝跃,周毅,刘维平,朱德康,刘菲,黄诚,宋涌.间接免疫酶组织化学法检测雏鹅感染鸭疫里默氏杆菌的研究.中国兽医杂志,2004,40(9):10-14牧兽医,1994,增刊:4-6
94.钱忠明,田惠芳,蒋志伟,庄国宏,陈义平,王永坤.肉鸭鸭疫巴氏杆菌病抗原型的研究及其防治:Ⅰ肉鸭鸭疫巴氏杆菌病的流行病学调查及诊断.中国家禽,1997,10:1-2
95.秦春雷,张大丙.鸭疫里默氏菌不同血清型在直接和间接荧光抗体试验中的交叉反应.中国兽医杂志,2006,42(8):25-26
96.曲丰发,蔡畅,郑献进,张大丙.利用16S rDNA建立种特异性PCR快速检测鸭疫里默氏菌.微生物学报,2006,46(1):13-17
97.曲丰发,张大丙,郑献进,郭玉璞.鸭疫里默氏菌16S rDNA基因的PCR-RFLP分析.中国兽医杂志,2005,41:11-14
98.曲永生,高睿,孙健,赵玲玲,赵晓春,孙刚.黑龙江省部分地区鸭疫里默氏杆菌病的流行病学调查与防制.黑龙江畜牧兽医,2002,5,25-26
99.沈关心,周汝麟主编.现代免疫学实验技术.武汉:湖北科学技术出版社,1998
100.石楠,张利平.新的系统发育标记及其应用.微生物学报,2003,30:112-115
101.苏敬良,黄瑜,吕艳丽,郭玉璞,孙艳争,樊丽红.间接荧光抗体技术检测鸭疫里氏杆菌.中国兽医杂志,1999,25(2):12-13
102.苏敬良,郭玉璞,吕艳丽.鸭疫里默氏杆菌免疫原性研究:Ⅱ荚膜提取物免疫原性测定.中国兽医杂志,1998b,24(8):3-5
103.苏敬良,郭玉璞,吕艳丽.鸭疫里氏杆菌免疫原性的研究:Ⅰ荚膜的形态学观察及荚吻质的提取.中国兽医杂志,1998a,24(1):8-10
104.苏敬良,郭玉璞,吕艳丽.鸭疫里氏杆菌外膜蛋白免疫原性研究.畜牧兽医学报,1999,30:444-448
105.苏敬良,黄瑜,吕艳丽,孙艳争,郭玉璞鸭传染性浆膜炎油佐剂灭活疫苗的研究 中国兽医科技,2000,30(6):12-14
106.唐发书,陈思怀.鸭传染性浆膜炎疫苗的研究进展.广东畜牧兽医科技2007,32(2):21-23
107.唐满华,黄青云,周萍,郭业锋.禽大肠埃希氏菌078和巴氏杆菌(5:A)外膜蛋白的免疫研究.中国兽医科技.2004,34(12):41-44
108.汪铭书,程安春,陈孝跃,刘兆宇,方鹏飞,郭宇飞.血清3型鸭疫里默氏杆菌在我国的发现及其病原特性研究.中国家禽,2001,23(22):9-12
109.汪铭书,程安春,陈孝跃,刘兆宇,方鹏飞,郭宇飞.血清5型鸭疫里氏杆菌在我国的发 现及其病原特性研究.中国兽医科技,2002,32(9):16-19
110.汪铭书,程安春,陈孝跃,刘兆宇,方鹏飞,郭宇飞.血清8型鸭疫里墨氏杆菌在我国的发现及其病原特性研究.中国预防兽医学报,2003,25(1):47-52
111.江铭书,程安春,李淑梅,朱德康,陈孝跃.RAPD用于鸭疫里默氏杆菌鸭源致病性大肠杆菌和鸭沙门氏菌的遗传相似性及聚类分析的初步研究.中国兽医杂志,2007,43(9):9-11
112.王春霞,王林川.广东省肇庆地区鸭疫里氏杆菌病的调查.中国兽医科技,2001,21:11-12
113.韦强,鲍国连,季权安,俞坚群,盛楚江,章红兵.鸭疫里氏杆菌形态特征的电镜观察.畜牧兽医学报,2004,35:314-317
114.韦强,鲍国连,王一成,徐丽华,季权安.鸭疫里氏杆菌赘生物提取及其理化特性研究.浙江大学学报(农业与生命科学版),2005,31(3):250-254.
115.温娟,梁伯先,李锦泉.广西南宁市鸭疫里氏杆菌病的调查与诊治.中国兽医科技,2002,32(7):35-37
116.吴华,李胜利,侯百枝,陈华,胡功政.鸭疫里氏杆菌病药物防治研究进展.动物医学进展,2007,28:96-100
117.吴礼洁,陈泽祥.鸭疫里氏杆菌不同血清型广西株的分离鉴定,中国兽医科技,2001,31(3):13-14
118.吴南洋,江斌,吴胜会,林琳.氟甲碱霉素对鸭大肠杆菌和鸭疫里默氏杆菌体外抑菌试捡及临床应用效果.福建畜牧兽医,2000,22:9
119.肖海君,闫振贵,崔金生,刘冠华,王小娥,杨春晓,朱瑞良.支气管败血波氏杆菌外膜蛋白的提取及其免疫效果的检测.微生物学通报,2008,35(9):1426-1432
120.肖运才.禽流感病毒ELISA快速检测试剂盒的研制及其重组核蛋白粘膜免疫研究.[博士学位论文].湖北武汉,华中农业大学,2005
121.熊涛,李自力,毕丁仁.鸭疫里氏杆菌的分离与鉴定.长江大学学报,2006,3(3):141-147
122.鄢志强,颜晓芸,潘金谷,王林川.鸭疫里氏杆菌保存条件探讨.黑龙江畜牧兽医,2004,2:38
123.杨鸿,苏子珩,林奕,杨少林,王敏儒,张风仙.常用抗菌药物对珠三角部分地区鸭疫里默氏杆菌敏感性调查.养禽与禽病防治,2006,12:2-3
124.杨建远,邓舜洲,何后军.PCR法快速检测鸭疫里氏杆菌的研究.江西农业大学学报,2005,27(3):39-442
125.杨建远,张良慧,过七根,张炳火,何后军.江西地区鸭疫里氏杆菌的鉴定及药敏试验,中国家禽,2006,28(24):110-115
126.张大丙,郭玉璞.1株难以定型的鸭疫里默氏菌分离株.中国兽医学报,2005b,25(2):148-151
127.张大丙,郭玉璞.15型鸭疫里氏杆菌分离株的鉴定.中国兽医杂志,2002b,38(3):23
128.张大丙,曲丰发,郑献进,郭玉璞.血清10型鸭疫里默氏菌第5个血清亚型的分析.中国兽医杂志,2005c,41(4):3-6
129.张大丙,郑献进,曲丰发.鸭疫里默氏菌一个可能新型的鉴定.2006,28(1):98-100
130.张大丙,郭玉璞.7型鸭疫里氏杆菌分离株的鉴定.中国兽医杂志,2003,39(5):18-19
131.张大丙,郭玉璞.北京地区鸭病毒性肝炎与传染性浆膜炎的流行情况与防制现状.中国兽医杂志,1993,19(12):11-13
132.张大丙,郭玉璞.北京地区鸭传染性浆膜炎的流行病学调查.中国预防兽医学报,1999a,21(4):260-263
133.张大丙,郭玉璞.北京地区鸭疫里氏杆菌及其血清型的分布.中国兽医杂志,1998,24(4):15-17
134.张大丙,郭玉璞.不同血清型鸭疫里氏杆菌分离株的部分特性比较.中国兽医杂志,1999b,25(10):13-15
135.张大丙,郭玉璞.鸭传染性浆膜炎的流行病学调查.中国兽医杂志,1999c,25(12):25
136.张大丙,郭玉璞.鸭疫里默氏菌6型、12型与16型之间的交叉反应.中国兽医学报,2002c,22(6):565-567
137.张大丙,郭玉璞.鸭疫里氏杆菌2型与17型之间交叉反应的研究.中国预防兽医学报,2002a,24(3):192-194
138.张大丙.不同参照菌和不同试验方法对鸭疫里默氏菌分型的影响.中国兽医杂志,2004,40(10):12-14
139.张大丙.血清10型鸭疫里默氏菌4个亚型的分析.畜牧兽医学报,2005a,36(2):181-186
140.张冬冬,张大丙.用间接ELISA检测鸭疫里默氏菌不同血清型之间的交叉反应.中国兽医杂志,2006,42(6):23-25
141.张鹤晓,郭玉璞.间接ELISA检测鸭疫里氏杆菌抗体的研究.中国畜禽传染病,1998,20(3):183-186
142.周淑兰,曹国文,付利芝,姜永康.鸭疫里默氏杆菌的分离鉴定和药物敏感性试验.黑龙江畜牧兽医,2002,9:34
143.朱德康.五种血清型鸭疫里默氏杆菌外膜蛋白特性比较及其免疫原性的研究.[博士学位论文].四川雅安,四川农业大学,2003
144.朱立平,陈学清主编.免疫学常用实验方法.北京:人民军医出版社,2000
145.朱琪,张加勇,刘晓辉.应用间接免疫荧光抗体试验检测鸭疫里氏杆菌,黑龙江兽医杂志,2001,30:31-34
146.Abiko Y,Hayakawa M,Murai S,Takiguchi H.Glycylprolyl dipeptidylaminopeptidase from Bacteroides gingivalis.Dent Res,1985,64:106-111
147.Adams M A,Singh V K,Keller B O,Jia Z.Structural and biochemical characterization of gentisate 1,2-dioxygenase from Escherichia coli O157:H7. Molecular Microbiology, 2006, 61, 1469-1484
148. Akerley B J, Rubin E J, Camilli A, Lampe D J, Robertson H M, Mekalanos J J. Systematic identification of essential genes by invitro mariner mutagenesis. Proc Natl Acad Sci USA, 1998, 95: 8927-8932
149. Alm R A, Manning PA. Characterization of the hlyB gene and its role in the production of the EI Tor haemolysin of Vibrio cholerae Ol.Mol Microbiol, 1990, 4(3): 413-425
150. Ames G F, Mimura C S, Shyamala V. Bacterial periplasmic permeases belong to a family of transport proteins operating from Escherichia coli to human: Traffic ATPases. FEMS Microbiol Rev, 1990, 6(4): 429-446
151. Andreasen J R, Sandhu T. Pasteurella anatipestfer-like bacteria associated with respiratory diseases in pigeons. Avian Diseases, 1993, 37: 908-911
152. Anthony R M, Brown T J, French G L. Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array. J Clin Microbiol, 2000,38(2):781-788
153. Arias-Barrau E, Olivera E R, Luengo J M, Fernandez C, Galan B, Garcia J L, Diaz E, Minambres B. The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida. The Journal of Bacteriology, 2004, 186, 5062-5077
154. Augustyns K G, Bal G, Thonus A, Belyaev X M, Zhang W, Bollaert A M. The unique properties of dipeptidyl-peptidase IV (DPPIV/CD26) and the therapeutic potential of DPPIV inhibitors. Curr. Med. Chem, 1999,6:311-327
155. Baggio L, Morrison M. The NAD(P)H-Utilizing Glutamate Dehydrogenase of Bacteroides thetaiotaomicron Belongs to Enzyme Family, and Its Activity Is Affected by trans-Acting Gene (s) Positioned Downstream of gdhA. Journal of Bacteriology, 1996, 178(24):7212 -7220
156. Baltes N, Tonpitak W, Gerlach G F, Hennig-Pauka I, Hoffmann-Moujahid A, Ganter M, Rothkotter H J. Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response. Infect Immun, 2001, 69:472-478
157. Baltes N, Tonpitak W, Hennig-Pauka I, Gruber A D, Gerlach G F. Actinobacillus pleuropneumoniae serotype 7 siderophore receptor FhuA is not required for virulence. FEMS Microbiol. Lett, 2003, 220:41-48
158. Bangun A, Johnson J L, Tripathy D N. Taxonomy of Pasteurella anatipestifer. 1. DNA base composition and DNA-DNA Hybridization analysis. Avian Diseases, 1987, 31(1):43-45
159. Bangun A, Tripathy D N, Hanson L E. Studies of Pasteurella anatipestifer. An approach to its classification. Avian Diseases, 1981, 25(2):326-337
160. Berger K H, Isberg R R. Two distinct defects in intracellular growth complemented by a single genetic locus in legionella pneurnophila. Mol Microbiol, 1993, 7: 7-19
161. Bernstein J A, Khodursky A B, Lin P H, Lin-Chao S, Cohen S N. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci USA, 2002, 99, 9697-9702
162. Bisgaard M. Antigenic studies on Pasteurella anatipestifer, species incertae sedis, using slide and tube agglutination. Avian Pathology, 1982, 11, 341-350
163. Boyce J D, Wilkie I, Harper M, Paustian M L, Kapur V, Adler B. Genomic scale analysis of Pasteurella multocida gene expression during growth within the natural chicken host. Infection and Immunity, 2002, 70, 6871-6879
164. Brogden K A , Rhoades K L , RimLer R B. Serologic types and physologic characteristic of 46 avian Pasteurella anatipestifer cultures. Avian Dis, 1982, 26:891-896.
165. Brunet D W. A Strain of Moraxella anatipestifer isolated form ducks. Cornell Vet, 1952, 44:461-464
166. Brunet D W, Fabricant J. A strain of Moraxella anatipestifer (Pfeifferella anatipestifer) isolated form ducks. Cornell Vet, 1954, 44:461-464
167. Chang C F, Hung P E, Chang Y F. Molecular characterization of a plasmid isolated from Riemerella anatipestifer. Avian pathology, 1998, 27(4): 339-345
168. Cheng S, Clancy C J, Checkley M A, Handheld M, Hillman J D, Progulske-Fox A, Lewin A S, Fidel P L, Nguyen M H. Identification of Candida albicans genes induced during thrush offers insight into pathogenesis. Mol Microbiol, 2003, 48:1275-1288
169. Chiang S L, Mekalanos J J, Holden D W. In vivo genetic analysis of bacterial virulence. Annual Review of Microbiology, 1999, 53, 129-154
170. Cooper G L. Pasteurella anatipestifer infectiond in California turkey flocks circumstantial evidence of a mosquito vector. Avian Diseases, 1989, 33: 809-815
171. Crasta K C, Chua K L, Subramaniam S, Frey J, Loh H, Tan H M. Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer. The Journal of Bacteriology, 2002, 184, 1932-1939
172. Daigle F, Graham J E, Curtiss R III. Identification of Salmonella typhi genes expressed within macrophages by selective capture of transcribed sequences (SCOTS). Mol Microbiol, 2001,41 (5):1211-1222
173. Deb D K, Dahiya P, Srivastava K K, Srivastava R, Srivastava B S. Selective identification of new therapeutic targets of Mycobacterium tuberculosis by IVIAT approach. Tuberculosis (Edinb), 2002,82:175-182
174. De Lay N R, Cronan J E. Gene-Specific Random Mutagenesis of Escherichia coli In Vivo: Isolation of Temperature-Sensitive Mutations in the Acyl Carrier Protein of Fatty Acid Synthesis. Journal of bacteriology, 2006, 188(1): 287-296
175. Dougherty E, Saunders L Z, Parsons E H. The pathology of infectious serostis of ducks. Am J Pathol, 1955,31:475-487
176. Dozois C M, Daigle F, Curtiss R III. Identification of pathogenspecific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain. Proc. Natl. Acad. Sci. USA , 2003, 100:247-252
177. Fang H M, Ge R, Sin Y M. Cloning, characterisation and expression of Aeromonas hydrophila major adhesion. Fish Shellfish Immunoi. 2004, 16(5): 645-58
178. Faucher S P, Porwollik S, Dozois C M, McClelland M, Daigle F. Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences. Proc. Natl. Acad. Sci. USA, 2006, 103, 1906-1911
179. Frank V, Eva V, James F D, Dirk I. The role of active oxygen species in plant signal transduction. Plant Science, 2001. 161: 405-414
180. Glunder G, Hinz K H. Isolation of Moraxella anatipestifer from embryonated goose eggs. Avian pathology, 1989, 18:351-355
181. Goldstein J M, BanbuLa A, KorduLa T, Mayo J A, Travis J. Novel extracellμlar x-prolyl dipeptidyl-peptidase (DPP) from Streptococcus gordonii FSS2: an emerging subfamily of viridans streptococcal x-prolyl DPPs. Infect Immun, 2001, 69:5494-5501
182. Gottesman S. Proteases and their targets in Escherichia coli. Annu. Rev. Genet, 1996, 30:465-506
183. Graham J E, Clark-Curtiss J E. Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS). Proc Natl Acad Sci USA, 1999, 96:11554-11559
184. Graham J E, Peek R M, Krishna U, Cover T L. Global analysis of Helicobacter pylori gene expression in human gastric mucosa. Gastroenterology, 2002, 123, 1637-1648
185. Handfield M, Brady L J, Progulske-FoxA, Hillman J D. IVIAT: a novel method to identify microbial genes expressed specifically during human infections. Trends in Microbiology, 2000, 8, 336-339
186. Hang L, John M, Asaduzzaman M, Bridges E A, Vanderspurt C, Kirn T J, Taylor R K, Hillman J D, Progulske-Fox A, Handfield M, Ryan E T, Calderwood S B. Use of in vivo induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibriocholerae. Proc Natl Acad Sci USA, 2003, 100: 8508-8513
187. Harris J B, Baresch-Bernal A, Rollins S M, Alam A, Larocque R C, Bikowski M, Peppercorn A F, Handheld M, Hillman J D, Qadri F, Calderwood S B, Hohmann E,Breiman R F, Brooks W A, Ryan E T. Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi. Infect Immune, 2006, 74: 5161-5168
188. Harry E G. Pasteurella anatipestifer serotypes isolated from cases of Riemerella anatipestefer septicaemia in ducks. Vet Rec, 1969, 84: 673
189. Hatfield R M, Morris B A, Henry R R. Development of an enzyme-linked immunosorbent assay for the detection of humoral antibody to Pasteurella anatipestifer. Avian Pathol, 1988, 16:123-140
190. Heifer D H, Helmboldt C F. Pasteurella anatipestifer infection in Turkeys. Avian Diseases, 1977, 21(4): 712-715
191. Helling R B. Pathway choice in glutamate synthesis in Escherichia coli. The Journal of Bacteriology, 1998, 180,4571-4575
192. Hendrickson J M, Hilbert KF.A new and serious septicemic disease of young ducks with a description of the causative organism Riemerella anatiestifer. N. S. Cornell Vet, 1932, 22:239-252
193. Hensel M, Shea J E, Gleeson C, Jones M D, Dalton E, Holden D W. Simultaneous identification of bacterial virulence genes by negative selection. Science, 1995, 269, 400-403
194. Hou J Y, Graham J E, Clark-Curtiss J E. Mycobacterium avium genes expressed during growth in human macrophages detected by selective capture of transcribed sequences (SCOTS). Infect. Immun, 2002, 70:3714-3726
195. Huang B, Subramaniam S, Chua K L, Kwang J, Loh H, Frey J, Tan H M. Molecular fingerprinting of Riemerella anatipestifer by repetitive sequence PCR. Veterinary Microbiology, 1999,67,213-219 identification of bacterial virulence genes by negative selection. Science, 1995, 269: 400-403
196. Jager J, Pauptit R A. Three dimensional structure of a mutant E.coli aspartate amino transferase with increased enzymic activity. Protein Engineering, 1994, 7 (5):605-612
197. Kawai K, Liu Y, Ohnishi K, Oshima S. A conserved 37 kD outer membrane protein of Edwardsiella tarda is an effective vaccine candidate. Vaccine 2004, 22(25-26): 3411-3418
198. Khushiramani R, Girisha S K, Karunasagar I. Prorective efficacy of recombinant OmpTS protein of Aeromonas hydrophila in Indian major carp. Vaccine,2007, 25(7): 1157-1158
199. Kim Y R, Lee S E, Kim C M, Kim S Y, Shin E K, Shin D H, Chung S S, Choy H E, Progulske-Fox A, Hillman J D, Handfield M, Rhee J H. Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients Infect Immun, 2003, 71:5461-5471
200. Kumagai Y, Konishi K, Gomi T, Yagishita H, Yajima A, And Yoshikawa M. Enzymatic properties of dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis and its participation in viruLence. Infect. Immun, 2000, 68, 716-724
201. Kumagai Y, Yagishita H, Yajima, A. Molecular mechanism for connective tissue destruction by dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis. Infection and Immunity, 2005, 73(26):55-64
202. Lamarche M G, Dozois C M, Daigle F, Caza M, CurtissIII R, Dubreuil J Danie, Harel J. Inactivation of the Pst System Reduces the Virulence of an Avian Pathogenic Escherichia coli O78 Strain. Infection and Immunity, 2005, 73(7): 4138-4145
203. Lisitsyn N, Wigler M. Cloning the differences between genomes, Science, 1993, 259, 946-951
204. Liu S Q, Graham J E, Bigelow L, Morse II P D, Wilkinson B J. Identification of Listeria monocytogenes genes expressed in response to growth at low temperature. Appl. Environ. Microbiol, 2002, 68(4): 1697-1705
205. Liu Y, Oshima S, Kurohara K, Ohnishi K, Kawai K. Vaccine efficacy of recombinant GAPDH of Edwardsiella tarda against Edwardsiellosis. Microbiol Immunol. 2005, 49(7): 605-12
206. Loh H, Teo T P, Tan H. Serotypes of Pasteurella anatipestiper isolated form ducks in Singapore: a proposal of new serotypes. Avian Pathology, 1992, 21:453-459
207. Ludwig W, Kirchhof G, Klugbauer N. Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G+C content. J. Syst Appl Microbiol, 1992, 15:487-501.
208. Lyerly D M, Barroso L A, Wilkins T D. Identification of the latex test-reactive protein of Clostridium difficile as glutamate dehydrogenase. Clin. Microbiol, 1991, 29:2639-2642
209. Mahan M j, Slauch J M, Mekalanos J J. Selection of bacterial virulence genes that are specifically induced in host tissues. Science, 1993, 259, 686-688
210. Mannheim W. Family III. Pasteurellaceae Pohl 1981a, 382. In N. R. Krieg and J. G. Holt eds. Bergey' Manual of Systematic Bacteriology, 9th ed., vol. Williams & Wilkins, Baltimore. MD, 1984, pp,550-557
211. Marshak D R, Kadonaga J T, Burgess R R, Knuth M W, Brennan Jr W A, Lin S H. Strategies for protein purification and characterization: A Laboratory course manual. New York: Cold Spring Harbor Laboratory Press, 1996
212. Mekalanos J J. Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol, 1992, 174(1): 1-7
213. Milcamps A, de Bruijn F J. Identification of a novel, nutrient deprivation induced gene (hmgA) in Sinorhizobium meliloti, involved in tyrosine degradation. Microbiology, 1999, 145, 935-947
214. Modrich P. Mechanisms and biological effects of mismatch repair. Annu Rev Genet, 1991, 25: 229-253
215. Mori E, Fulchieri M, Indorato C, Fani R, Bazzicalupo M. Cloning, nucleotide sequencing, and expression of the Azospirillum brasilense lon gene: involvement in iron uptake. J Bacteriol, 1996, 178:3440-3446
216. Munday B L, Corbould A, Heddleston K L, Harry E G. Isolation of Pasteurella anatipestifer from Black Swan (Cygnus atratus). Aust Vet J, 1970, 46(7):322-325
217. Nausch I, Heymann E. Substance P in human plasma is degraded by dipeptidyl peptidase IV, not by cholinesterase. Neurochem, 1985, 44:1354-1357
218. Ogawa N, Tzeng C M, Fraley C D, Kornberg A. Inorganic polyphosphate in Vibrio cholerae: genetic, biochemical, and physiologic features. The Journal of Bacteriology, 2000, 182, 6687-6693
219. Ogi Okwumabua, JuLia S, Persaud P G. Cloning and Characterization of the Gene Encoding the Glutamate Dehydrogenase of Streptococcus suis Serotype 2. Clin Diagn Lab Immunol, 2001, 8(2):251-257
220. Pathanasophon P, Phuektes P, Tanticharoenyos T, Narongsak W, Sawada T. A potential new serotype of Riemerella anatipestifer isolated from ducks in Thailand. Avian Pathology, 2002, 31, 267-270
221. Pathanasophon P, Sawada T, Pramoolsinsap T, Tanticharoenyos T. Immunogenicity of Riemerella anatipestifer broth culture bacterin and cell-free culture filtrate in ducks. Avian Pathology, 1996a, 25, 705-719
222. Pathanasophon P, Tanticharoenyos T. study on the immunogenecity of Riemerella anatipestifer. Journal of the Thai Veterinary Medical Association, 1996b, 47(1): 13-20
223. Pathanasophon P, Tanticharoenyos T, Sawada T. Physiological characteristics, antimicrobial susceptibility and serotypes of Pasteurella anatipestifer isolated from ducks in Thailand. Veterinary Microbiology, 1994, 39, 179-185
224. Pathansophon P, Sawada T, Tanticharonenyos T. New serotypes of Riemerella isolated from ducks in Thaiand. Avian Pathology, 1995, 24:195-199
225. Piechulla K, Pohl S, Mannheim W. Phenotypic and genetic relationships of so-called Moraxella (Pasteurella) anatipestifer to the Flavobacterium/Cytophaga group. Vet. Microbiol, 1986 , 11(3):261-270
226. Pierce R L, Vorhies M W. Pasteurella anatipestifer infection in geese. Avian Diseases, 1973, 17 (4):868-870
227. Pourmand M R, Clarke S R, Schuman R F, Mond J J, Foster S J. Identification of Antigenic Components of Staphylococcus epidermidis Expressed during Human Infection. Infection and Immunity, 2006, 74(8): 4644-4654
228. Radcliff F J, Hazell S L, Kolesnikow T, Doidge C, Lee A. Catalase, a novel antigen for Helicobacter pylori vaccination. Infect Immun, 1997, 65(11): 4668-4674
229. Rashid M H, Rumbaugh K, Passador L, Davies D G, Hamood AN, Iglewski B H, Kornberg V. Polyphosphate kinase is essential for biofilm development, quorum sensing, and virulence of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA, 2000, 97, 9636-9641
230. RimLer R B, Nordholm G E. DNA fingerprinting of Riemerella anatipestifer. Avian Disease, 1998,42,101-105.
231. Robertson, G. T., Kovach M E, Allen C A, Ficht T A, RoopII R M. The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB/c mice. Mol Microbiol, 2000, 35:577-588
232. Rosenfeld L E. Pasteurella anatipestifer infection in fowls in Australia. Aust Vet J, 1973, 49(1): 55-56
233. Ryll M, Christensen H, Bisgaard M, Christensen J P, Hinz K H, Kohler B. Studies on the prevalence of Riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains. J. Vet. Med. B, 2001, 48, 537-546
234. Sandhu T S, Harry E G. Serotypes of Pasteurella anatipestifer isolated from commercial White Pekin ducks in the United States. Avian Dis, 1981, 25(2):497-502
235. Sandhu T S, Layton H W. Laboratory and field trials with formalin-inactivated Escherichia coli (O_(78))-Pasteurella anatipestifer bacterin in white pekin ducks. Avian Dis, 1985, 29(1): 128-35
236. Sandhu T S, Leister M L. Serotypes of Pasteurella anatipestifer isolated from poultry in different countries. Avian Pathology, 1991, 20:233-239
237. Sandhu T S. Immunization of white pekin ducklings against Pasteurella anatipestifer infection. Avian Disease, 1979, 23(2):662-669
238. Sandhu T S. Immunogenicity and safety of a live Pasteurella anatipestifer vaccine in White Pekin ducklings: laboratory and field trials. Avian Pathology, 1991, 20, 423-432
239. Sarver C F, Morishita T Y, Nersessian B. The effect of route of inoculation and challenge dosage on Riemerella anatipestifer infection in Pekin Ducks (Anas platyrhynchos). Avian Diseases, 2002, 49, 104-107.
240. Scherf U, Buckel W. Purification and Properties of 4-Hydroxybutyrate Coenzyme A Transferase from Clostridium aminobutyricum. Applied and enviromental microbiology, 1991, 57(9): 2699-2702
241. Schmidt R, Decatur A L, Rather P N, Moran C P, Jr, Losick R. Bacillus subtilis Lon protease prevents inappropriate transcription of genes under the control of the sporulation transcription factor σ~G. J Bacteriol, 1994, 176:6528-6537
242. Schoemaker J M, Randall C G, Markovitz A. Regulation of cell division in Escherichia coli: SOS induction and cellular location of the SulA protein, a key to /on-associated filamentation and death. J Bacteriol, 1984, 158:551-561
243. Segers P, Mannheim W, Vancanneyt M, De Brandt K, Hinz K H, Kersters K, Vandamme P. Riemerella anatipestifer gen. nov., comb.nov., the causative agent of septicaemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group. International Journal of Systematic and Evolutionary Microbiology, 1993, 43, 768-776.
244. Smith A J, Savery N J. RNA polymerase mutants defective in the initiation of transcription-coupled DNA repair. Nucleic Acids Research, 2005, 33(2): 755-764
245. Smith J M, Frame D D, Cooper G, Bickford A A, Ghazikhanian G Y, Kelly B J. Pasteurella anatipestifer infection in Commercial Meat-Type Turkeys in California. Avian Diseases, 1987, 31(4):913-917
246. Soo P C, Horng Y T, Lai M J, Wei J R, Hsieh S C, Chang Y L, Tsai Y H, Lai H C. Pirin Regulates Pyruvate Catabolism by Interacting with the Pyruvate Dehydrogenase E1 Subunit and Modulating Pyruvate Dehydrogenase Activity. Journal of bacteriology, 2007, 189(1): 109-118
247. Stanley P, Hyland C, Koronakis V, Hughes C. An ordered reaction mechanism for bacterial toxin acylation by the specialized acyltransferase HlyC: formation of a ternary complex with acylACP and protoxin substrates. Molecular Microbiology, 1999, 34(5): 887-901
248. Stewart B J, Enos-Berlage J L, McCarter L L. The lonS gene regulates swarmer cell differentiation of Vibrio parahaemolyticus. J Bacteriol, 1997, 179:107-114
249. Subramaniam S, Chua K L, Tan H M, Loh H, Kuhnert P, Frey J. Phylogenetic position of R. anatipestifer based on 16S rRNA gene sequences. International Journal of Systematic and Evolutionary Microbiology, 1997, 47, 562-565.
250. Subramaniam S, Huang B, Loh H, Kwang J, Tan H, Chua K, Frey J. Characterization of a predominant immunogenic outer membrane protein of Riemerella anatipestifer. Clin Diagn Lab Immun,2000, 7(2): 168-174
251. Takaya A, Tomoyasu T, Tokumitsu A, Morioka M, Yamamoto T. The ATP-Dependent Lon Protease of Salmonella enterica Serovar Typhimurium Regulates Invasion and Expression of Genes Carried on Salmonella Pathogenicity Island 1. Journal of bacteriology, 2002, 184(1): 224-232
252. Tojo N, Inouye S, Komanq T. The lonD gene is homologous to the Ion gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus. J Bacteriol, 1993, 175:4545-4549
253. Torres-Cabassa A S, Gottesman S. Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis. J. Bacteriol, 1987, 169:981-989
254. Tiireci O, Luxemburger U, Heinen H, Mack U, Sybrecht G W, Huber C, Sahin U. CrELISA: a fast and robust enzyme-linked immunosorbent assay bypassing the need for purification of recombinant protein. J Immuno Meth, 2004, 289: 191-199
255. Valdivia R H, Falkow S. Fluorescence-based isolation of bacterial genes expressed within host cells. Science, 1997, 277, 2007-2011
256. Valdvia R H, Falkow S. Fluorescence-based isolation of bacterial genes expressed within hpost cells. Science, 1997, 277: 2007-2011
257. Webster S P, Alexeev D, Campopiano D J, Watt R M, Alexeeva M, Sawyer L, Baxter R L. Mechanism of 8-Amino-7-oxononanoate Synthase: Spectroscopic, Kinetic, and Crystallographic Studies. Biochemistry, 2000, 39, 516-528
258. Wen Z, Morrison M. The NAD(P)H Dependent Glutamate Dehydrogenase Activitiesof Prevotella ruminicola B14 Can Be Attributed to One Enzyme (GdhA), and gdhA Expression Is Regulated in Response to the Nitrogen Source Available for Growth. Appliedand Environmental Microbiology, 1996,62:3826-3833
259. Weng S, Lin W, Chang Y, Chang C. Identification of a virulence-associated protein homolog gene and ISRal in a plasmid of Riemerella anatipestifer. FEMS Microbiology Letters, 1999, 179, 11-19
260. Wilson M, DeRisi J, Kristensen H H, Imboden P, Rane S, Brown P O, Schoolnik G K. Exploring drug-induced alteration in gene expression in Mycobacterium tuberculosis by microarray hybridization. Proc. Natl. Acad. Sci. USA, 1999, 96, 12833-12838.
261. Wobeser G, Ward G E. Pasteurella anatipestifer infection in migrating whistling swans. J. Wild Dis, 1974, 10(4):466-470
262. Wright R, Stephens C., Zweiger G, Shapiro L, Alley M R. Caulobacter Lon protease has a critical role in cell-cycle control of DNA methylation. Genes Dev, 1996, 15:1532-1542
263. Yagishita H, Kumagai Y, Konishi K, Takahashi Y, Aoba T, Yoshikawa M. Histopathological studies on virulence of dipeptidyl aminopeptidase IV (DPPIV) of Porphyromonas gingivalis in a mouse abscess model: use of a DPPIV-deficient mutant. Infect. Immun, 2001, 69, 7159-7161
264. Yoonsuk P, Yilmaz O, Jung I Y, Lamont R J.Identification of Porphyromonas gingivalis Genes Specifically Expressed in Human Gingival Epithelial Cells by Using Differential Display Reverse Transcription-PCR. Infection and Immunity, 2004, 72(7): 3752-3758
265. Yu C Y, Liu Y W, Chou S J, Chao M R, Weng B C, Tsay J G, Chiu C H, Ching Wu C, Long Lin T, Chang C C, Chu C. Genomic diversity and molecular differentiation of Riemerella anatipestifer associated with eight outbreaks in five farms. Avian Pathol, 2008, 37(3):273-279
266. Zhao Y X, Jansen R, Gaastra W, Arkesteijn G, van der Zeijst Bernard A M, van Putten Jose P M. Identification of Genes Affecting Salmonella enterica Serovar Enteritidis Infection of Chicken Macrophages. Infection and Immunity, 2002, 70(9): 5319-5321
2.白洁,李雪飞,黄波,李莹,王波,吕申.DNA错配修复基因产物表达与肿瘤恶性程度相关性的初步探讨.大连医科大学学报,1998,20(4):24-25
3.鲍国连,佟承刚,韦强,季全安,费炳荣,陶锡荣,姜为民.鸭传染性浆膜炎流行病学与病原特性研究.浙江农业学报,1997,9(3):161-164
4.鲍国连,佟承刚,韦强,季全安,费炳荣,陶锡荣,姜为民.鸭传染性浆膜炎疫苗的研究.中国预防兽医学报,1999,21(6):414-416
5.毕利军,周亚凤,邓教宇,张先恩,张成刚.DNA错配修复系统研究进展.生物化学与生物物理进展,2003,30(1):32-37
6.蔡畅,曲丰发,郑献进,丁春宇,张大丙.鸭疫里默氏菌16S rDNA基因的单链构象多态性分析.中国兽医杂志,2006,42(4):10-12
7.蔡家利,唐晓丽,杨龙峰,苏晓运。鸭传染性浆膜炎蜂胶疫苗研制.中国兽医学报,2001,21(6):552-553
8.蔡家利,詹晓庆.鸭疫里氏杆菌和大肠杆菌二联灭活疫苗研究.中国家禽,2004,26(20):10-12
9.蔡秀磊,韦强,崔言顺,鲍国连,霍翠梅,季权安,李建亮,张先福.鸭疫里默氏杆菌临床分离株的耐药性与Ⅰ型整合子检测.中国预防兽医学报,2007,29(9):727-731
10.曹素芳,黄青云.禽多杀性巴氏杆菌C_(48-1),成熟外膜蛋白重组亚单位疫苗免疫效果的研究.中国兽医科学,2006,36(6):464-467
11.陈芬芬.鸭疫里氏杆菌体内诱导抗原基因的筛选与应用研究.[硕士学位论文].湖北武汉,华中农业大学,2008
12.陈师勇,莫照兰,张振东,邹玉霞,徐永立,张培军.细菌毒力基因体内表达检测技术研究进展.遗传,2005,27(3):505-511
13.陈兴生,李布勇.海南省鸭疫里默氏杆菌流行病学调查.中国预防兽医学报,1999,21(3):231-233
14.程安春,汪铭书,陈孝跃,钟妮娜,赖为民.鸭疫巴氏杆菌的分离和鉴定.四川畜牧兽医,1996,2:7-9
15.程安春,汪铭书,陈孝跃,朱德康,黄城,刘菲,周毅,郭宇飞,刘兆宇,方鹏飞.我国鸭疫里默菌血清型调查及新血清型的发现和病原特性.中国兽医学报,2003b,23:320-323
16.程安春,汪铭书,方鹏飞,郭宇飞,刘兆宇,陈孝跃,周毅.间接酶联免疫吸附试验(ELISA)检测血清4型鸭疫里默氏杆菌抗体的研究.中国家禽,2004b,16:14-17
17.程安春,汪铭书,方鹏飞,郭宇飞,刘兆字,陈孝跃,周毅.间接酶联免疫吸附试验((ELISA)检测血清2型鸭疫里默氏杆菌抗体的研究.中国兽医杂志,2004a,40:14-17
18.程安春,汪铭书,郭宇飞,方鹏飞,刘兆宇,陈孝跃,周毅.鸭疫里默氏菌铝胶佐剂与铝胶复合佐剂4价灭活疫苗的比较.中国兽医学报,2005,25(2):152-156
19.程安春,汪铭书,郭宇飞,朱德康,陈孝跃,刘维平,孟琼华,李淑梅.鸭疫里墨氏杆菌病及其在我国的流行动态和防治对策.中国家禽,2003a,22:29-31
20.程安春,汪铭书,刘兆宁,周毅,陈孝跃,方鹏飞,郭宇飞.血清2型鸭疫里默氏杆菌外膜蛋白多肤的组成及免疫原性.中国兽医学报,2004c,24:329-331
21.程龙飞,傅光华,施少华,陈红梅,彭春香,黄瑜,林芳,林建生.Ⅰ型鸭疫里氏杆菌基因组质粒表达文库的构建.江西农业大学学报,2007,29(6):887-890
22.程龙飞,施少华,李文杨,傅光华,彭春香,黄瑜.1、2型鸭疫里默氏菌外膜蛋白分析.福建农林大学学报(自然科学版),2005,34(2):260-262
23.程龙飞,施少华,傅光华,李文杨,彭春香,黄瑜,陈溥言.2型鸭疫里氏杆菌抗体间接ELISA检测方法的建立与应用.福建农业学报,2006,21(2):131-134
24.程龙飞,施少华,傅光华,彭春香,黄瑜,陈红梅,万春和.2型鸭疫里默氏菌抗体效价与免疫保护率的相关性测定.福建畜牧兽医,2009,31(2):25-26
25.程龙飞,施少华,李文杨,傅光华,黄瑜.PCR检测鸭疫里氏菌的研究.江西农业大学学报,2004,26(1):131-133
26.程龙飞,钟敏,郑腾,傅光华,陈红梅,施少华,白泉阳,黄瑜.鸭疫里氏杆菌敏感烈性噬菌体的分离鉴定.中国兽医科学,2009,39(02):106-109
27.邓舜洲,叶林方,何后军,朱芝秀,谢伟东.江西地区鸭疫里氏杆菌微生物学特性的研究.江西农业大学学报(自然科学版),2002,24:502-507
28.董传甫,林天龙,龚晖,欧阳岁东,杨苏.嗜水气单胞菌主要外膜蛋白对欧洲鳗鲡的免疫保护试验.水生生物学报,2005,29(3):285-290
29.杜念兴主编.兽医免疫学,第二版.北京:中国农业出版社,1997
30.范京惠,左玉柱,李潭清,宋勤叶,赵坤乾.鸭疫里默氏杆菌河北株的分离鉴定及药敏试验.养禽与禽病防治,2007,9,30-31
31.房宝志,孙晓燕,殷廷秀.微山县首次发现鸭传染性浆膜炎.山东畜牧兽医,2000,2:43
32.高福,郭玉璞,程锡麟.小鸭传染性浆膜炎疫苗的研究:II鸭疫巴氏杆菌灭活苗的初步研究.中国兽医杂志,1987,13(4):51-53
33.高福,郭玉璞,张应国.小鸭传染性浆膜炎疫苗的研究:Ⅲ鸭疫巴氏杆菌矿物油佐剂灭活苗.中国兽医杂志,1990,16:46-48
34.高福,郭玉璞.小鸭传染性浆膜炎疫苗的研究:Ⅰ鸭疫巴氏杆菌的血清型鉴定.中国兽医杂志,1987,13(4):47-48
35.高崧,吴晓东,张扬,刘秀梵,张如宽.禽大肠杆菌外膜蛋白、脂多糖疫苗的免疫保护试验.中国兽医学报,2002,22(5):457-459
36.顾宏伟,陆承平.兔多杀性巴氏杆菌铁调节外膜蛋白的小鼠免疫效果分析.中国农业科学,2007,40(5):1073-1078
37.郭春祥和郭锡琼.介绍一种简单、快速、高效的辣根过氧化物酶标记抗体的过碘酸钠法.上海免疫学杂志,1983,3(2):97-100
38.郭予强,杨连楷,凌育粲,詹乐群.鸭疫巴氏杆菌病及其并发感染的防治.养禽与禽病防治,1994,3:28-29
39.郭予强.鸭疫综合征的研究.华南农学院学报,1983,4(2):11-20
40.郭宇飞,程安春,汪铭书,方鹏飞,刘兆宇,陈孝跃,周毅.鸭疫里氏杆菌病四价灭活疫苗的研究.中国兽医科技,2003,33(6):3-9
41.郭玉璞,陈德威,陈俊杰.鸭疫巴氏杆菌和多杀巴氏杆菌在电子显微镜下的比较研究.北京农业大学学报,1986,12(1):261-270
42.郭玉璞,陈德威,范国雄,刘瑞萍.北京鸭鸭传染性浆膜炎的调查研究.畜牧兽医学报,1982,13(2):107-112
43.郭玉璞,甘孟侯,张中直.应用荧光抗体法诊断小鸭传染性英膜炎的试验.中国畜禽传染病,1998,20(3):183-186
44.何昭阳,胡桂学,王春风主编.动物免疫学实验技术.长春:吉林科学技术出版社,2002,59-61。
45.洪帮兴,江丽芳,张世英,司徒潮满,林奕芝,钟跃星,胡玉山.病原菌23S rRNA基因序列变异性分析.疾病控制杂志,2004,8(5):385-388
46.胡清海,李刚,郑明球,蔡宝祥.间接ELISA检测鸭疫里氏杆菌抗体的研究.中国畜禽传染病,1998,20(6):354-356
47.胡清海,李刚,郑明球,蔡宝祥.鸭疫里氏杆菌江苏株G+C含量同源性研究.南京农业大学学报,1998,21(4):87-91
48.胡清海,刘晓文,苗晋锋,张知良,丁铲,章耀良.一株鹅源鸭疫里氏杆菌与鸭源分离株的致病性比较.畜牧与兽医,2002,34(9):3-4
49.胡清海,刘晓文,赵世华,张知良,丁铲,苗晋锋,刘华雷,赵东伟.应用PCR技术检测鸭疫里氏杆菌的研究.中国预防兽医学报,2002,24(6):471-473
50.胡清海,张知良,黄建芳.野鸭鸭疫里氏杆菌的分离和鉴定.中国家禽,2001,23(7):10-11
51.胡清海,张知良,苗晋锋,刘岳龙,刘晓文,丁铲.江苏安徽两省鸭疫里默氏杆菌病的流行病学调查研究.中国兽医科技,2001,31(8):12-13
52.胡晓娜,朱瑞良,刘红珍,毕建敏.禽波氏杆菌外膜蛋白的提取及其免疫原性的检测.微生物学报,2007,47(4):714-717
53.胡薛英,谷长勤,程国富,李自力,周诗其,毕丁仁,赵雅心.人工感染鸭疫里氏杆菌雏鸭的病理学变化.中国兽医科技,2004,34(3).48-50
54.黄庆洲,郭定章,但麒麟,林明明,伍莉.川渝两地鸭疫里氏杆菌分离鉴定及药效试验.四川畜牧兽医,2002,29(1):19-20
55.黄淑坚,王政富,梁文冠,吴志新,龚中贵,张莹,梁家桥,陈燕萍.粤北地区鸭疫里默氏杆菌的分离鉴定及药敏试验.中国家禽,2007,29(24):53-54
56.黄瑜,李文扬,程龙飞,庄向生,苏敬良,吕艳丽,郭玉璞.福建省血清Ⅱ型鸭疫里氏杆菌病的诊治.中国预防兽医学报,1999,21(1):
57.黄瑜,林世棠.鸭疫巴氏杆菌病的研究进展及存在的问题.中国家禽,1994,5:34-35
58.黄瑜,苏敬良,李文杨,程龙飞,彭春香,施少华,郭玉璞.1型鸭疫里默氏菌3种不同佐剂灭活苗的比较.中国兽医学报,2002.22:561-563
59.柯岩,陈哲生.制备兔抗人IgG抗体的一种简便方法.微生物学通报.1997,24(5):315-317
60.黎德兵,崔恒敏,章蕾.鸭疫里默氏杆菌病发病机理的研究:Ⅰ人工感染雏鸭部分血液指标的动态变化.黑龙江畜牧兽医,2004a,1:8-10
61.黎德兵,崔恒敏,章蕾.鸭疫里默氏杆菌病发病机理的研究:Ⅱ人工感染雏鸭病理形态学变化.黑龙江畜牧兽医,2004b,2:10-12
62.黎德兵,崔恒敏,赵翠燕.鸭疫里默氏杆菌感染后雏鸭血清生化指标变化的研究.畜禽业,2007,223(11):26-27
63.黎满香,董伟,雷红宇,宁玲忠.鸭疫里默氏菌和大肠杆菌混合感染的诊断及防制.畜牧兽医杂志,2004,23(3):46-47
64.黎晓敏,郭丽敏,唐发书,王健.鸭传染性浆膜炎病鸭免疫器官的光镜及电镜观察.畜牧兽医学报,2004,35(6):702-704
65.李继祥,徐刚,王孝友,杜华茂,黄伟.重庆地区鸭传染性浆膜炎病原分离鉴定.中国预防兽医学报,2000,22(6):402-422
66.李家奎,郭定宗,程大池,杨世锦,刘庆龙.武汉地区鸭疫巴氏杆菌的分离鉴定.中国预防兽医学报,2000,22:423-424
67.李康然.鸭疫巴氏杆菌感染.广西畜牧兽医,1996,12(3):52-52
68.李霆,高志勇,王恒梁,冯尔玲,陈宗德,李新月,黄留玉,黄翠芬.应用体内诱导抗原技术筛选结核分枝杆菌体内表达基因.遗传学报,2005,32(2):111-117
69.李文杨,程龙飞,彭春香,黄瑜.福建省鸭疫里默氏菌血清流行病学调查.中国预防兽医学报,2002,24(2):138-139
70.李晓霞,邱玉玉,王海荣.肠致病性大肠杆菌外膜蛋白免疫保护性研究.中国免疫学杂志,2007,23(5):394-397
71.李自力,毕丁仁,许青荣,石德时,程峰,刘梅.鸭传染牲浆膜炎的诊断与防治.湖北畜
牧兽医,2003,4:34-35
72.李自力,熊涛,毕丁仁,石德时,胡薛英,许青荣,程峰.湖北鸭疫里氏杆菌分离株人工感染试验及灭活疫苗研制.湖北农业科学,2004,5:87-89
73.梁月丽,陶海静.鸭疫巴氏杆菌的分离与鉴定.河南畜牧兽医,2003,24:7-8
74.廖立新,曹郁生,李国辉,陈燕,张万山,熊勇华,姚闽.铜绿假单胞菌外膜蛋白的免疫原性及免疫保护性.中华微生物学和免疫学杂志,2004,24(7):571-572
75.林丽超,陈建红,黄盛,张济培,张元跃.鸭疫里氏杆菌疫苗在雏鸭体内诱导细胞免疫的动态研究.佛山科学技术学院学报(自然科学版),2005,23(3):73-76
76.林琳,吴南洋,江斌,吴胜会.儿种药物对鸭疫里氏杆菌的抑菌试验.福建畜牧兽医,2000,22(2):11-12
77.林世堂,郁晓岚,黄瑜.鸭疫巴氏杆菌亚单位成分的提取及免疫原性初步研究.福建畜
78.林禧龄,曾彦钦.福州首次发现鸭疫巴氏杆菌病.中国兽医杂志,1992,18(9):12-13
79.林业杰,林禧龄,曾颜钦.福州首次从康贝尔鸭分离出鸭疫巴氏杆菌.中国兽医科技,1989,2:26-27
80.刘维平,程安春,江铭书,陈孝跃,周毅,朱德康,刘菲,孟琼华,黄诚,宋涌.间接免疫组化法检测鸭疫里默氏杆菌感染和抗原定位.中国兽医学报,2005,25(4):362-365
81.刘文华,苏敬良,刘颖,许秀梅,金鑫,吴培福.16s rRNA基因及外膜蛋白基因分析在鸭疫里莫氏杆菌鉴定中的应刚.中国预防兽医学报,2006,28(6):691-696
82.刘小艳.氟苯尼考注射液治疗鸭疫里默氏杆菌病的疗效试验.吉林畜牧兽医,2004,6:51
83.刘颖,苏敬良,刘文华,李云格.鸭疫里默氏菌的药物敏感性检测.中国兽医杂志,2005,41(5):10-12
84.刘颖,苏敬良,刘文华.鸭疫里默氏菌的gyrA基因的检测与变异位点的分析.中国兽医杂志,2007,43(1):12-14
85.刘永德,吴祖立,叶陈梁,高莉莉.鸭疫巴氏杆菌与大肠杆菌二联苗研究.中国畜禽传染病,1997,93(2):4-6
86.陆承平.兽医微生物学第三版.中国农业出版社,2001,9
87.吕殿红,张毓金,干刚.鸭疫里氏杆菌病的研究进展.动物医学进展,2000,21(4):189-192
88.吕殿红.鸭疫里默氏杆菌菌种保存试验及蜂胶苗的保护试验.动物医学进展,2003,23(1):79-81
89.吕敏娜,黄承锋,张玉团,张健马非,杨海金.鸭传染性浆膜炎荚膜多糖苗研究初报.广东畜牧兽医科技,1999,3:16-18
90.吕敏娜,黄承锋,张毓金.鸭疫里默氏杆菌大肠杆菌二联蜂胶苗的研制.中国兽医杂志,2005,41:48-50
91.罗东生.我省首次发现小鸭传染性浆膜炎.湖南畜牧兽医,1997,6:31
92.毛娅卿,翁崇鹏,刘烈发,章振华,堪南辉.三肤囊素Bursin对鸭疫里氏杆菌病灭活苗免疫效果的影响.江西农业大学学报,2005,27:282-284
93.孟琼华,程安春,汪铭书,陈孝跃,周毅,刘维平,朱德康,刘菲,黄诚,宋涌.间接免疫酶组织化学法检测雏鹅感染鸭疫里默氏杆菌的研究.中国兽医杂志,2004,40(9):10-14牧兽医,1994,增刊:4-6
94.钱忠明,田惠芳,蒋志伟,庄国宏,陈义平,王永坤.肉鸭鸭疫巴氏杆菌病抗原型的研究及其防治:Ⅰ肉鸭鸭疫巴氏杆菌病的流行病学调查及诊断.中国家禽,1997,10:1-2
95.秦春雷,张大丙.鸭疫里默氏菌不同血清型在直接和间接荧光抗体试验中的交叉反应.中国兽医杂志,2006,42(8):25-26
96.曲丰发,蔡畅,郑献进,张大丙.利用16S rDNA建立种特异性PCR快速检测鸭疫里默氏菌.微生物学报,2006,46(1):13-17
97.曲丰发,张大丙,郑献进,郭玉璞.鸭疫里默氏菌16S rDNA基因的PCR-RFLP分析.中国兽医杂志,2005,41:11-14
98.曲永生,高睿,孙健,赵玲玲,赵晓春,孙刚.黑龙江省部分地区鸭疫里默氏杆菌病的流行病学调查与防制.黑龙江畜牧兽医,2002,5,25-26
99.沈关心,周汝麟主编.现代免疫学实验技术.武汉:湖北科学技术出版社,1998
100.石楠,张利平.新的系统发育标记及其应用.微生物学报,2003,30:112-115
101.苏敬良,黄瑜,吕艳丽,郭玉璞,孙艳争,樊丽红.间接荧光抗体技术检测鸭疫里氏杆菌.中国兽医杂志,1999,25(2):12-13
102.苏敬良,郭玉璞,吕艳丽.鸭疫里默氏杆菌免疫原性研究:Ⅱ荚膜提取物免疫原性测定.中国兽医杂志,1998b,24(8):3-5
103.苏敬良,郭玉璞,吕艳丽.鸭疫里氏杆菌免疫原性的研究:Ⅰ荚膜的形态学观察及荚吻质的提取.中国兽医杂志,1998a,24(1):8-10
104.苏敬良,郭玉璞,吕艳丽.鸭疫里氏杆菌外膜蛋白免疫原性研究.畜牧兽医学报,1999,30:444-448
105.苏敬良,黄瑜,吕艳丽,孙艳争,郭玉璞鸭传染性浆膜炎油佐剂灭活疫苗的研究 中国兽医科技,2000,30(6):12-14
106.唐发书,陈思怀.鸭传染性浆膜炎疫苗的研究进展.广东畜牧兽医科技2007,32(2):21-23
107.唐满华,黄青云,周萍,郭业锋.禽大肠埃希氏菌078和巴氏杆菌(5:A)外膜蛋白的免疫研究.中国兽医科技.2004,34(12):41-44
108.汪铭书,程安春,陈孝跃,刘兆宇,方鹏飞,郭宇飞.血清3型鸭疫里默氏杆菌在我国的发现及其病原特性研究.中国家禽,2001,23(22):9-12
109.汪铭书,程安春,陈孝跃,刘兆宇,方鹏飞,郭宇飞.血清5型鸭疫里氏杆菌在我国的发 现及其病原特性研究.中国兽医科技,2002,32(9):16-19
110.汪铭书,程安春,陈孝跃,刘兆宇,方鹏飞,郭宇飞.血清8型鸭疫里墨氏杆菌在我国的发现及其病原特性研究.中国预防兽医学报,2003,25(1):47-52
111.江铭书,程安春,李淑梅,朱德康,陈孝跃.RAPD用于鸭疫里默氏杆菌鸭源致病性大肠杆菌和鸭沙门氏菌的遗传相似性及聚类分析的初步研究.中国兽医杂志,2007,43(9):9-11
112.王春霞,王林川.广东省肇庆地区鸭疫里氏杆菌病的调查.中国兽医科技,2001,21:11-12
113.韦强,鲍国连,季权安,俞坚群,盛楚江,章红兵.鸭疫里氏杆菌形态特征的电镜观察.畜牧兽医学报,2004,35:314-317
114.韦强,鲍国连,王一成,徐丽华,季权安.鸭疫里氏杆菌赘生物提取及其理化特性研究.浙江大学学报(农业与生命科学版),2005,31(3):250-254.
115.温娟,梁伯先,李锦泉.广西南宁市鸭疫里氏杆菌病的调查与诊治.中国兽医科技,2002,32(7):35-37
116.吴华,李胜利,侯百枝,陈华,胡功政.鸭疫里氏杆菌病药物防治研究进展.动物医学进展,2007,28:96-100
117.吴礼洁,陈泽祥.鸭疫里氏杆菌不同血清型广西株的分离鉴定,中国兽医科技,2001,31(3):13-14
118.吴南洋,江斌,吴胜会,林琳.氟甲碱霉素对鸭大肠杆菌和鸭疫里默氏杆菌体外抑菌试捡及临床应用效果.福建畜牧兽医,2000,22:9
119.肖海君,闫振贵,崔金生,刘冠华,王小娥,杨春晓,朱瑞良.支气管败血波氏杆菌外膜蛋白的提取及其免疫效果的检测.微生物学通报,2008,35(9):1426-1432
120.肖运才.禽流感病毒ELISA快速检测试剂盒的研制及其重组核蛋白粘膜免疫研究.[博士学位论文].湖北武汉,华中农业大学,2005
121.熊涛,李自力,毕丁仁.鸭疫里氏杆菌的分离与鉴定.长江大学学报,2006,3(3):141-147
122.鄢志强,颜晓芸,潘金谷,王林川.鸭疫里氏杆菌保存条件探讨.黑龙江畜牧兽医,2004,2:38
123.杨鸿,苏子珩,林奕,杨少林,王敏儒,张风仙.常用抗菌药物对珠三角部分地区鸭疫里默氏杆菌敏感性调查.养禽与禽病防治,2006,12:2-3
124.杨建远,邓舜洲,何后军.PCR法快速检测鸭疫里氏杆菌的研究.江西农业大学学报,2005,27(3):39-442
125.杨建远,张良慧,过七根,张炳火,何后军.江西地区鸭疫里氏杆菌的鉴定及药敏试验,中国家禽,2006,28(24):110-115
126.张大丙,郭玉璞.1株难以定型的鸭疫里默氏菌分离株.中国兽医学报,2005b,25(2):148-151
127.张大丙,郭玉璞.15型鸭疫里氏杆菌分离株的鉴定.中国兽医杂志,2002b,38(3):23
128.张大丙,曲丰发,郑献进,郭玉璞.血清10型鸭疫里默氏菌第5个血清亚型的分析.中国兽医杂志,2005c,41(4):3-6
129.张大丙,郑献进,曲丰发.鸭疫里默氏菌一个可能新型的鉴定.2006,28(1):98-100
130.张大丙,郭玉璞.7型鸭疫里氏杆菌分离株的鉴定.中国兽医杂志,2003,39(5):18-19
131.张大丙,郭玉璞.北京地区鸭病毒性肝炎与传染性浆膜炎的流行情况与防制现状.中国兽医杂志,1993,19(12):11-13
132.张大丙,郭玉璞.北京地区鸭传染性浆膜炎的流行病学调查.中国预防兽医学报,1999a,21(4):260-263
133.张大丙,郭玉璞.北京地区鸭疫里氏杆菌及其血清型的分布.中国兽医杂志,1998,24(4):15-17
134.张大丙,郭玉璞.不同血清型鸭疫里氏杆菌分离株的部分特性比较.中国兽医杂志,1999b,25(10):13-15
135.张大丙,郭玉璞.鸭传染性浆膜炎的流行病学调查.中国兽医杂志,1999c,25(12):25
136.张大丙,郭玉璞.鸭疫里默氏菌6型、12型与16型之间的交叉反应.中国兽医学报,2002c,22(6):565-567
137.张大丙,郭玉璞.鸭疫里氏杆菌2型与17型之间交叉反应的研究.中国预防兽医学报,2002a,24(3):192-194
138.张大丙.不同参照菌和不同试验方法对鸭疫里默氏菌分型的影响.中国兽医杂志,2004,40(10):12-14
139.张大丙.血清10型鸭疫里默氏菌4个亚型的分析.畜牧兽医学报,2005a,36(2):181-186
140.张冬冬,张大丙.用间接ELISA检测鸭疫里默氏菌不同血清型之间的交叉反应.中国兽医杂志,2006,42(6):23-25
141.张鹤晓,郭玉璞.间接ELISA检测鸭疫里氏杆菌抗体的研究.中国畜禽传染病,1998,20(3):183-186
142.周淑兰,曹国文,付利芝,姜永康.鸭疫里默氏杆菌的分离鉴定和药物敏感性试验.黑龙江畜牧兽医,2002,9:34
143.朱德康.五种血清型鸭疫里默氏杆菌外膜蛋白特性比较及其免疫原性的研究.[博士学位论文].四川雅安,四川农业大学,2003
144.朱立平,陈学清主编.免疫学常用实验方法.北京:人民军医出版社,2000
145.朱琪,张加勇,刘晓辉.应用间接免疫荧光抗体试验检测鸭疫里氏杆菌,黑龙江兽医杂志,2001,30:31-34
146.Abiko Y,Hayakawa M,Murai S,Takiguchi H.Glycylprolyl dipeptidylaminopeptidase from Bacteroides gingivalis.Dent Res,1985,64:106-111
147.Adams M A,Singh V K,Keller B O,Jia Z.Structural and biochemical characterization of gentisate 1,2-dioxygenase from Escherichia coli O157:H7. Molecular Microbiology, 2006, 61, 1469-1484
148. Akerley B J, Rubin E J, Camilli A, Lampe D J, Robertson H M, Mekalanos J J. Systematic identification of essential genes by invitro mariner mutagenesis. Proc Natl Acad Sci USA, 1998, 95: 8927-8932
149. Alm R A, Manning PA. Characterization of the hlyB gene and its role in the production of the EI Tor haemolysin of Vibrio cholerae Ol.Mol Microbiol, 1990, 4(3): 413-425
150. Ames G F, Mimura C S, Shyamala V. Bacterial periplasmic permeases belong to a family of transport proteins operating from Escherichia coli to human: Traffic ATPases. FEMS Microbiol Rev, 1990, 6(4): 429-446
151. Andreasen J R, Sandhu T. Pasteurella anatipestfer-like bacteria associated with respiratory diseases in pigeons. Avian Diseases, 1993, 37: 908-911
152. Anthony R M, Brown T J, French G L. Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array. J Clin Microbiol, 2000,38(2):781-788
153. Arias-Barrau E, Olivera E R, Luengo J M, Fernandez C, Galan B, Garcia J L, Diaz E, Minambres B. The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida. The Journal of Bacteriology, 2004, 186, 5062-5077
154. Augustyns K G, Bal G, Thonus A, Belyaev X M, Zhang W, Bollaert A M. The unique properties of dipeptidyl-peptidase IV (DPPIV/CD26) and the therapeutic potential of DPPIV inhibitors. Curr. Med. Chem, 1999,6:311-327
155. Baggio L, Morrison M. The NAD(P)H-Utilizing Glutamate Dehydrogenase of Bacteroides thetaiotaomicron Belongs to Enzyme Family, and Its Activity Is Affected by trans-Acting Gene (s) Positioned Downstream of gdhA. Journal of Bacteriology, 1996, 178(24):7212 -7220
156. Baltes N, Tonpitak W, Gerlach G F, Hennig-Pauka I, Hoffmann-Moujahid A, Ganter M, Rothkotter H J. Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response. Infect Immun, 2001, 69:472-478
157. Baltes N, Tonpitak W, Hennig-Pauka I, Gruber A D, Gerlach G F. Actinobacillus pleuropneumoniae serotype 7 siderophore receptor FhuA is not required for virulence. FEMS Microbiol. Lett, 2003, 220:41-48
158. Bangun A, Johnson J L, Tripathy D N. Taxonomy of Pasteurella anatipestifer. 1. DNA base composition and DNA-DNA Hybridization analysis. Avian Diseases, 1987, 31(1):43-45
159. Bangun A, Tripathy D N, Hanson L E. Studies of Pasteurella anatipestifer. An approach to its classification. Avian Diseases, 1981, 25(2):326-337
160. Berger K H, Isberg R R. Two distinct defects in intracellular growth complemented by a single genetic locus in legionella pneurnophila. Mol Microbiol, 1993, 7: 7-19
161. Bernstein J A, Khodursky A B, Lin P H, Lin-Chao S, Cohen S N. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci USA, 2002, 99, 9697-9702
162. Bisgaard M. Antigenic studies on Pasteurella anatipestifer, species incertae sedis, using slide and tube agglutination. Avian Pathology, 1982, 11, 341-350
163. Boyce J D, Wilkie I, Harper M, Paustian M L, Kapur V, Adler B. Genomic scale analysis of Pasteurella multocida gene expression during growth within the natural chicken host. Infection and Immunity, 2002, 70, 6871-6879
164. Brogden K A , Rhoades K L , RimLer R B. Serologic types and physologic characteristic of 46 avian Pasteurella anatipestifer cultures. Avian Dis, 1982, 26:891-896.
165. Brunet D W. A Strain of Moraxella anatipestifer isolated form ducks. Cornell Vet, 1952, 44:461-464
166. Brunet D W, Fabricant J. A strain of Moraxella anatipestifer (Pfeifferella anatipestifer) isolated form ducks. Cornell Vet, 1954, 44:461-464
167. Chang C F, Hung P E, Chang Y F. Molecular characterization of a plasmid isolated from Riemerella anatipestifer. Avian pathology, 1998, 27(4): 339-345
168. Cheng S, Clancy C J, Checkley M A, Handheld M, Hillman J D, Progulske-Fox A, Lewin A S, Fidel P L, Nguyen M H. Identification of Candida albicans genes induced during thrush offers insight into pathogenesis. Mol Microbiol, 2003, 48:1275-1288
169. Chiang S L, Mekalanos J J, Holden D W. In vivo genetic analysis of bacterial virulence. Annual Review of Microbiology, 1999, 53, 129-154
170. Cooper G L. Pasteurella anatipestifer infectiond in California turkey flocks circumstantial evidence of a mosquito vector. Avian Diseases, 1989, 33: 809-815
171. Crasta K C, Chua K L, Subramaniam S, Frey J, Loh H, Tan H M. Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer. The Journal of Bacteriology, 2002, 184, 1932-1939
172. Daigle F, Graham J E, Curtiss R III. Identification of Salmonella typhi genes expressed within macrophages by selective capture of transcribed sequences (SCOTS). Mol Microbiol, 2001,41 (5):1211-1222
173. Deb D K, Dahiya P, Srivastava K K, Srivastava R, Srivastava B S. Selective identification of new therapeutic targets of Mycobacterium tuberculosis by IVIAT approach. Tuberculosis (Edinb), 2002,82:175-182
174. De Lay N R, Cronan J E. Gene-Specific Random Mutagenesis of Escherichia coli In Vivo: Isolation of Temperature-Sensitive Mutations in the Acyl Carrier Protein of Fatty Acid Synthesis. Journal of bacteriology, 2006, 188(1): 287-296
175. Dougherty E, Saunders L Z, Parsons E H. The pathology of infectious serostis of ducks. Am J Pathol, 1955,31:475-487
176. Dozois C M, Daigle F, Curtiss R III. Identification of pathogenspecific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain. Proc. Natl. Acad. Sci. USA , 2003, 100:247-252
177. Fang H M, Ge R, Sin Y M. Cloning, characterisation and expression of Aeromonas hydrophila major adhesion. Fish Shellfish Immunoi. 2004, 16(5): 645-58
178. Faucher S P, Porwollik S, Dozois C M, McClelland M, Daigle F. Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences. Proc. Natl. Acad. Sci. USA, 2006, 103, 1906-1911
179. Frank V, Eva V, James F D, Dirk I. The role of active oxygen species in plant signal transduction. Plant Science, 2001. 161: 405-414
180. Glunder G, Hinz K H. Isolation of Moraxella anatipestifer from embryonated goose eggs. Avian pathology, 1989, 18:351-355
181. Goldstein J M, BanbuLa A, KorduLa T, Mayo J A, Travis J. Novel extracellμlar x-prolyl dipeptidyl-peptidase (DPP) from Streptococcus gordonii FSS2: an emerging subfamily of viridans streptococcal x-prolyl DPPs. Infect Immun, 2001, 69:5494-5501
182. Gottesman S. Proteases and their targets in Escherichia coli. Annu. Rev. Genet, 1996, 30:465-506
183. Graham J E, Clark-Curtiss J E. Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS). Proc Natl Acad Sci USA, 1999, 96:11554-11559
184. Graham J E, Peek R M, Krishna U, Cover T L. Global analysis of Helicobacter pylori gene expression in human gastric mucosa. Gastroenterology, 2002, 123, 1637-1648
185. Handfield M, Brady L J, Progulske-FoxA, Hillman J D. IVIAT: a novel method to identify microbial genes expressed specifically during human infections. Trends in Microbiology, 2000, 8, 336-339
186. Hang L, John M, Asaduzzaman M, Bridges E A, Vanderspurt C, Kirn T J, Taylor R K, Hillman J D, Progulske-Fox A, Handfield M, Ryan E T, Calderwood S B. Use of in vivo induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibriocholerae. Proc Natl Acad Sci USA, 2003, 100: 8508-8513
187. Harris J B, Baresch-Bernal A, Rollins S M, Alam A, Larocque R C, Bikowski M, Peppercorn A F, Handheld M, Hillman J D, Qadri F, Calderwood S B, Hohmann E,Breiman R F, Brooks W A, Ryan E T. Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi. Infect Immune, 2006, 74: 5161-5168
188. Harry E G. Pasteurella anatipestifer serotypes isolated from cases of Riemerella anatipestefer septicaemia in ducks. Vet Rec, 1969, 84: 673
189. Hatfield R M, Morris B A, Henry R R. Development of an enzyme-linked immunosorbent assay for the detection of humoral antibody to Pasteurella anatipestifer. Avian Pathol, 1988, 16:123-140
190. Heifer D H, Helmboldt C F. Pasteurella anatipestifer infection in Turkeys. Avian Diseases, 1977, 21(4): 712-715
191. Helling R B. Pathway choice in glutamate synthesis in Escherichia coli. The Journal of Bacteriology, 1998, 180,4571-4575
192. Hendrickson J M, Hilbert KF.A new and serious septicemic disease of young ducks with a description of the causative organism Riemerella anatiestifer. N. S. Cornell Vet, 1932, 22:239-252
193. Hensel M, Shea J E, Gleeson C, Jones M D, Dalton E, Holden D W. Simultaneous identification of bacterial virulence genes by negative selection. Science, 1995, 269, 400-403
194. Hou J Y, Graham J E, Clark-Curtiss J E. Mycobacterium avium genes expressed during growth in human macrophages detected by selective capture of transcribed sequences (SCOTS). Infect. Immun, 2002, 70:3714-3726
195. Huang B, Subramaniam S, Chua K L, Kwang J, Loh H, Frey J, Tan H M. Molecular fingerprinting of Riemerella anatipestifer by repetitive sequence PCR. Veterinary Microbiology, 1999,67,213-219 identification of bacterial virulence genes by negative selection. Science, 1995, 269: 400-403
196. Jager J, Pauptit R A. Three dimensional structure of a mutant E.coli aspartate amino transferase with increased enzymic activity. Protein Engineering, 1994, 7 (5):605-612
197. Kawai K, Liu Y, Ohnishi K, Oshima S. A conserved 37 kD outer membrane protein of Edwardsiella tarda is an effective vaccine candidate. Vaccine 2004, 22(25-26): 3411-3418
198. Khushiramani R, Girisha S K, Karunasagar I. Prorective efficacy of recombinant OmpTS protein of Aeromonas hydrophila in Indian major carp. Vaccine,2007, 25(7): 1157-1158
199. Kim Y R, Lee S E, Kim C M, Kim S Y, Shin E K, Shin D H, Chung S S, Choy H E, Progulske-Fox A, Hillman J D, Handfield M, Rhee J H. Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients Infect Immun, 2003, 71:5461-5471
200. Kumagai Y, Konishi K, Gomi T, Yagishita H, Yajima A, And Yoshikawa M. Enzymatic properties of dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis and its participation in viruLence. Infect. Immun, 2000, 68, 716-724
201. Kumagai Y, Yagishita H, Yajima, A. Molecular mechanism for connective tissue destruction by dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis. Infection and Immunity, 2005, 73(26):55-64
202. Lamarche M G, Dozois C M, Daigle F, Caza M, CurtissIII R, Dubreuil J Danie, Harel J. Inactivation of the Pst System Reduces the Virulence of an Avian Pathogenic Escherichia coli O78 Strain. Infection and Immunity, 2005, 73(7): 4138-4145
203. Lisitsyn N, Wigler M. Cloning the differences between genomes, Science, 1993, 259, 946-951
204. Liu S Q, Graham J E, Bigelow L, Morse II P D, Wilkinson B J. Identification of Listeria monocytogenes genes expressed in response to growth at low temperature. Appl. Environ. Microbiol, 2002, 68(4): 1697-1705
205. Liu Y, Oshima S, Kurohara K, Ohnishi K, Kawai K. Vaccine efficacy of recombinant GAPDH of Edwardsiella tarda against Edwardsiellosis. Microbiol Immunol. 2005, 49(7): 605-12
206. Loh H, Teo T P, Tan H. Serotypes of Pasteurella anatipestiper isolated form ducks in Singapore: a proposal of new serotypes. Avian Pathology, 1992, 21:453-459
207. Ludwig W, Kirchhof G, Klugbauer N. Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G+C content. J. Syst Appl Microbiol, 1992, 15:487-501.
208. Lyerly D M, Barroso L A, Wilkins T D. Identification of the latex test-reactive protein of Clostridium difficile as glutamate dehydrogenase. Clin. Microbiol, 1991, 29:2639-2642
209. Mahan M j, Slauch J M, Mekalanos J J. Selection of bacterial virulence genes that are specifically induced in host tissues. Science, 1993, 259, 686-688
210. Mannheim W. Family III. Pasteurellaceae Pohl 1981a, 382. In N. R. Krieg and J. G. Holt eds. Bergey' Manual of Systematic Bacteriology, 9th ed., vol. Williams & Wilkins, Baltimore. MD, 1984, pp,550-557
211. Marshak D R, Kadonaga J T, Burgess R R, Knuth M W, Brennan Jr W A, Lin S H. Strategies for protein purification and characterization: A Laboratory course manual. New York: Cold Spring Harbor Laboratory Press, 1996
212. Mekalanos J J. Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol, 1992, 174(1): 1-7
213. Milcamps A, de Bruijn F J. Identification of a novel, nutrient deprivation induced gene (hmgA) in Sinorhizobium meliloti, involved in tyrosine degradation. Microbiology, 1999, 145, 935-947
214. Modrich P. Mechanisms and biological effects of mismatch repair. Annu Rev Genet, 1991, 25: 229-253
215. Mori E, Fulchieri M, Indorato C, Fani R, Bazzicalupo M. Cloning, nucleotide sequencing, and expression of the Azospirillum brasilense lon gene: involvement in iron uptake. J Bacteriol, 1996, 178:3440-3446
216. Munday B L, Corbould A, Heddleston K L, Harry E G. Isolation of Pasteurella anatipestifer from Black Swan (Cygnus atratus). Aust Vet J, 1970, 46(7):322-325
217. Nausch I, Heymann E. Substance P in human plasma is degraded by dipeptidyl peptidase IV, not by cholinesterase. Neurochem, 1985, 44:1354-1357
218. Ogawa N, Tzeng C M, Fraley C D, Kornberg A. Inorganic polyphosphate in Vibrio cholerae: genetic, biochemical, and physiologic features. The Journal of Bacteriology, 2000, 182, 6687-6693
219. Ogi Okwumabua, JuLia S, Persaud P G. Cloning and Characterization of the Gene Encoding the Glutamate Dehydrogenase of Streptococcus suis Serotype 2. Clin Diagn Lab Immunol, 2001, 8(2):251-257
220. Pathanasophon P, Phuektes P, Tanticharoenyos T, Narongsak W, Sawada T. A potential new serotype of Riemerella anatipestifer isolated from ducks in Thailand. Avian Pathology, 2002, 31, 267-270
221. Pathanasophon P, Sawada T, Pramoolsinsap T, Tanticharoenyos T. Immunogenicity of Riemerella anatipestifer broth culture bacterin and cell-free culture filtrate in ducks. Avian Pathology, 1996a, 25, 705-719
222. Pathanasophon P, Tanticharoenyos T. study on the immunogenecity of Riemerella anatipestifer. Journal of the Thai Veterinary Medical Association, 1996b, 47(1): 13-20
223. Pathanasophon P, Tanticharoenyos T, Sawada T. Physiological characteristics, antimicrobial susceptibility and serotypes of Pasteurella anatipestifer isolated from ducks in Thailand. Veterinary Microbiology, 1994, 39, 179-185
224. Pathansophon P, Sawada T, Tanticharonenyos T. New serotypes of Riemerella isolated from ducks in Thaiand. Avian Pathology, 1995, 24:195-199
225. Piechulla K, Pohl S, Mannheim W. Phenotypic and genetic relationships of so-called Moraxella (Pasteurella) anatipestifer to the Flavobacterium/Cytophaga group. Vet. Microbiol, 1986 , 11(3):261-270
226. Pierce R L, Vorhies M W. Pasteurella anatipestifer infection in geese. Avian Diseases, 1973, 17 (4):868-870
227. Pourmand M R, Clarke S R, Schuman R F, Mond J J, Foster S J. Identification of Antigenic Components of Staphylococcus epidermidis Expressed during Human Infection. Infection and Immunity, 2006, 74(8): 4644-4654
228. Radcliff F J, Hazell S L, Kolesnikow T, Doidge C, Lee A. Catalase, a novel antigen for Helicobacter pylori vaccination. Infect Immun, 1997, 65(11): 4668-4674
229. Rashid M H, Rumbaugh K, Passador L, Davies D G, Hamood AN, Iglewski B H, Kornberg V. Polyphosphate kinase is essential for biofilm development, quorum sensing, and virulence of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA, 2000, 97, 9636-9641
230. RimLer R B, Nordholm G E. DNA fingerprinting of Riemerella anatipestifer. Avian Disease, 1998,42,101-105.
231. Robertson, G. T., Kovach M E, Allen C A, Ficht T A, RoopII R M. The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB/c mice. Mol Microbiol, 2000, 35:577-588
232. Rosenfeld L E. Pasteurella anatipestifer infection in fowls in Australia. Aust Vet J, 1973, 49(1): 55-56
233. Ryll M, Christensen H, Bisgaard M, Christensen J P, Hinz K H, Kohler B. Studies on the prevalence of Riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains. J. Vet. Med. B, 2001, 48, 537-546
234. Sandhu T S, Harry E G. Serotypes of Pasteurella anatipestifer isolated from commercial White Pekin ducks in the United States. Avian Dis, 1981, 25(2):497-502
235. Sandhu T S, Layton H W. Laboratory and field trials with formalin-inactivated Escherichia coli (O_(78))-Pasteurella anatipestifer bacterin in white pekin ducks. Avian Dis, 1985, 29(1): 128-35
236. Sandhu T S, Leister M L. Serotypes of Pasteurella anatipestifer isolated from poultry in different countries. Avian Pathology, 1991, 20:233-239
237. Sandhu T S. Immunization of white pekin ducklings against Pasteurella anatipestifer infection. Avian Disease, 1979, 23(2):662-669
238. Sandhu T S. Immunogenicity and safety of a live Pasteurella anatipestifer vaccine in White Pekin ducklings: laboratory and field trials. Avian Pathology, 1991, 20, 423-432
239. Sarver C F, Morishita T Y, Nersessian B. The effect of route of inoculation and challenge dosage on Riemerella anatipestifer infection in Pekin Ducks (Anas platyrhynchos). Avian Diseases, 2002, 49, 104-107.
240. Scherf U, Buckel W. Purification and Properties of 4-Hydroxybutyrate Coenzyme A Transferase from Clostridium aminobutyricum. Applied and enviromental microbiology, 1991, 57(9): 2699-2702
241. Schmidt R, Decatur A L, Rather P N, Moran C P, Jr, Losick R. Bacillus subtilis Lon protease prevents inappropriate transcription of genes under the control of the sporulation transcription factor σ~G. J Bacteriol, 1994, 176:6528-6537
242. Schoemaker J M, Randall C G, Markovitz A. Regulation of cell division in Escherichia coli: SOS induction and cellular location of the SulA protein, a key to /on-associated filamentation and death. J Bacteriol, 1984, 158:551-561
243. Segers P, Mannheim W, Vancanneyt M, De Brandt K, Hinz K H, Kersters K, Vandamme P. Riemerella anatipestifer gen. nov., comb.nov., the causative agent of septicaemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group. International Journal of Systematic and Evolutionary Microbiology, 1993, 43, 768-776.
244. Smith A J, Savery N J. RNA polymerase mutants defective in the initiation of transcription-coupled DNA repair. Nucleic Acids Research, 2005, 33(2): 755-764
245. Smith J M, Frame D D, Cooper G, Bickford A A, Ghazikhanian G Y, Kelly B J. Pasteurella anatipestifer infection in Commercial Meat-Type Turkeys in California. Avian Diseases, 1987, 31(4):913-917
246. Soo P C, Horng Y T, Lai M J, Wei J R, Hsieh S C, Chang Y L, Tsai Y H, Lai H C. Pirin Regulates Pyruvate Catabolism by Interacting with the Pyruvate Dehydrogenase E1 Subunit and Modulating Pyruvate Dehydrogenase Activity. Journal of bacteriology, 2007, 189(1): 109-118
247. Stanley P, Hyland C, Koronakis V, Hughes C. An ordered reaction mechanism for bacterial toxin acylation by the specialized acyltransferase HlyC: formation of a ternary complex with acylACP and protoxin substrates. Molecular Microbiology, 1999, 34(5): 887-901
248. Stewart B J, Enos-Berlage J L, McCarter L L. The lonS gene regulates swarmer cell differentiation of Vibrio parahaemolyticus. J Bacteriol, 1997, 179:107-114
249. Subramaniam S, Chua K L, Tan H M, Loh H, Kuhnert P, Frey J. Phylogenetic position of R. anatipestifer based on 16S rRNA gene sequences. International Journal of Systematic and Evolutionary Microbiology, 1997, 47, 562-565.
250. Subramaniam S, Huang B, Loh H, Kwang J, Tan H, Chua K, Frey J. Characterization of a predominant immunogenic outer membrane protein of Riemerella anatipestifer. Clin Diagn Lab Immun,2000, 7(2): 168-174
251. Takaya A, Tomoyasu T, Tokumitsu A, Morioka M, Yamamoto T. The ATP-Dependent Lon Protease of Salmonella enterica Serovar Typhimurium Regulates Invasion and Expression of Genes Carried on Salmonella Pathogenicity Island 1. Journal of bacteriology, 2002, 184(1): 224-232
252. Tojo N, Inouye S, Komanq T. The lonD gene is homologous to the Ion gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus. J Bacteriol, 1993, 175:4545-4549
253. Torres-Cabassa A S, Gottesman S. Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis. J. Bacteriol, 1987, 169:981-989
254. Tiireci O, Luxemburger U, Heinen H, Mack U, Sybrecht G W, Huber C, Sahin U. CrELISA: a fast and robust enzyme-linked immunosorbent assay bypassing the need for purification of recombinant protein. J Immuno Meth, 2004, 289: 191-199
255. Valdivia R H, Falkow S. Fluorescence-based isolation of bacterial genes expressed within host cells. Science, 1997, 277, 2007-2011
256. Valdvia R H, Falkow S. Fluorescence-based isolation of bacterial genes expressed within hpost cells. Science, 1997, 277: 2007-2011
257. Webster S P, Alexeev D, Campopiano D J, Watt R M, Alexeeva M, Sawyer L, Baxter R L. Mechanism of 8-Amino-7-oxononanoate Synthase: Spectroscopic, Kinetic, and Crystallographic Studies. Biochemistry, 2000, 39, 516-528
258. Wen Z, Morrison M. The NAD(P)H Dependent Glutamate Dehydrogenase Activitiesof Prevotella ruminicola B14 Can Be Attributed to One Enzyme (GdhA), and gdhA Expression Is Regulated in Response to the Nitrogen Source Available for Growth. Appliedand Environmental Microbiology, 1996,62:3826-3833
259. Weng S, Lin W, Chang Y, Chang C. Identification of a virulence-associated protein homolog gene and ISRal in a plasmid of Riemerella anatipestifer. FEMS Microbiology Letters, 1999, 179, 11-19
260. Wilson M, DeRisi J, Kristensen H H, Imboden P, Rane S, Brown P O, Schoolnik G K. Exploring drug-induced alteration in gene expression in Mycobacterium tuberculosis by microarray hybridization. Proc. Natl. Acad. Sci. USA, 1999, 96, 12833-12838.
261. Wobeser G, Ward G E. Pasteurella anatipestifer infection in migrating whistling swans. J. Wild Dis, 1974, 10(4):466-470
262. Wright R, Stephens C., Zweiger G, Shapiro L, Alley M R. Caulobacter Lon protease has a critical role in cell-cycle control of DNA methylation. Genes Dev, 1996, 15:1532-1542
263. Yagishita H, Kumagai Y, Konishi K, Takahashi Y, Aoba T, Yoshikawa M. Histopathological studies on virulence of dipeptidyl aminopeptidase IV (DPPIV) of Porphyromonas gingivalis in a mouse abscess model: use of a DPPIV-deficient mutant. Infect. Immun, 2001, 69, 7159-7161
264. Yoonsuk P, Yilmaz O, Jung I Y, Lamont R J.Identification of Porphyromonas gingivalis Genes Specifically Expressed in Human Gingival Epithelial Cells by Using Differential Display Reverse Transcription-PCR. Infection and Immunity, 2004, 72(7): 3752-3758
265. Yu C Y, Liu Y W, Chou S J, Chao M R, Weng B C, Tsay J G, Chiu C H, Ching Wu C, Long Lin T, Chang C C, Chu C. Genomic diversity and molecular differentiation of Riemerella anatipestifer associated with eight outbreaks in five farms. Avian Pathol, 2008, 37(3):273-279
266. Zhao Y X, Jansen R, Gaastra W, Arkesteijn G, van der Zeijst Bernard A M, van Putten Jose P M. Identification of Genes Affecting Salmonella enterica Serovar Enteritidis Infection of Chicken Macrophages. Infection and Immunity, 2002, 70(9): 5319-5321