不同浓度川芎嗪对吊尾大鼠比目鱼肌收缩功能的影响及机制的初步研究
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摘要
研究目的：
1、观察不同浓度川芎嗪对吊尾大鼠比目鱼收缩功能及肌浆网Ca2+-ATP酶活性的影响。
2、对川芎嗪影响大鼠比目鱼收缩功能的可能机制进行初步探讨。
研究方法：
1、尾部悬吊法建立大鼠废用性肌萎缩模型,使用PowerLab系统、电子刺激器(SEN.3301)等仪器测定不同浓度川芎嗪灌胃给药后,大鼠比目鱼肌肌肉收缩功能的变化。
2、利用定磷法测定不同浓度川芎嗪给药后,吊尾大鼠比目鱼肌肌浆网Ca2+-ATP酶活性。
研究结果：
1、与吊尾组比较：1)不同浓度川芎嗪灌胃给药组中,大鼠比目鱼肌的等长、强直收缩最大张力值均有显著增加,其中高浓度组等长收缩最大张力值增长179.02%,强直收缩最大张力值增长90.29%,低浓度组等长收缩最大张力值增长186.65%,强直收缩最大张力值增长123.61%。高低浓度之间无显著差异；2)不同浓度川芎嗪组收缩时程的收缩相TP50、TPT参数均显著增加,高浓度组分别增长148.09%和80.28%,低浓度组分别增长95.38%和31.67%,高低浓度之间无显著差异。3)高浓度川芎嗪组收缩时程的舒张相RT50参数增加21.36%(P<0.05)。
2、与吊尾组比较,灌胃给药川芎嗪后,高浓度组大鼠比目鱼肌肌浆网Ca2+-ATP酶活性升高55.60%(P<0.01),低浓度组升高71.84%(P<0.01)；不同浓度实验组中,低浓度组效果稍明显,但不具统计意义(P>0.05)。
研究结论：
1、不同浓度川芎嗪均能有效缓解吊尾造成的肌萎缩影响；有效对抗吊尾引发的比目鱼肌质网Ca2+-ATP酶活性降低。
2、川芎嗪可通过提高肌浆网Ca2+-ATP酶活性、抑制肌纤维类型转换等途径,对抗废用对肌肉收缩功能的影响。
Objectives:
1, To observe the effects of different concentrations of TMP on the soleus's contratility and on the sarcoplasmic reticulum Ca2+-ATPase activity in the tail-suspended rat.
2, To discuss the possible mechanism of TMP's effects on the soleus's contractility in rat
Methods:
1, The disuse-atrophy model was achieved by tail-suspension on rat; using PowerLab system and electronic stimulator (SEN.3301) to testify the soleus's contractility in rat.
2, The sarcoplasmic reticulum Ca2+-ATPase activity was measured by the Phosphate Determination method.
Results:
1, Compared with the tail-suspended group:1) both the peak twitch tension (Pt) and peak tetanic tension (Po) of soleus in rat have increased significantly in those groups of intragastric administration with different TMP concentrations; the Pt and P0 have increased 179.02%, 90.29% respectively in the high TMP concentration group, and have increased 186.65%, 123.61% respectively in the low TMP concentration group; there was no significant difference between the high and low TMP concentration groups.2) the time to 50% peak tension (TP50) and time to peak tension (TPT) have incerased notably in those groups of intragastric administration with different TMP concentrations; the TP50 and TPT have rised 148.09% and 80.28% respectively in the high TMP concentration group, and have increased 95.38% and 31.67% respectively in the low TMP concentration group; no significant changes was observed between the high and low TMP concentration groups.3) time from peak tension to 50% relaxation (RTso) has increased 21.36%(P<0.05) in the high TMP concentration group.
2, Compared with the tail-suspended group, after intragastric administration with TMP, the sarcoplasmic reticulum Ca2+-ATPase activity of the rat's soleus has increased 55.60%(P <0.01) and 71.84%(P<0.01) in high and low TMP concentration groups respectively; low TMP concentration was more effective than high one on the sarcoplasmic reticulum Ca2+-ATPase activity but was not statistically significant (P>0.05).
Conclusions:
1, Different concentrations of TMP can effectively alleviate the Tail on the impact caused by muscle atrophy; effective against the Tail on the soleus muscle induced endoplasmic reticulum Ca2+-ATP activity decreased.
2, TMP can increase sarcoplasmic reticulum Ca2+-ATP activity, inhibition of muscle fiber type conversion way, against the disuse of the muscle contraction function.
1、观察不同浓度川芎嗪对吊尾大鼠比目鱼收缩功能及肌浆网Ca2+-ATP酶活性的影响。
2、对川芎嗪影响大鼠比目鱼收缩功能的可能机制进行初步探讨。
研究方法：
1、尾部悬吊法建立大鼠废用性肌萎缩模型,使用PowerLab系统、电子刺激器(SEN.3301)等仪器测定不同浓度川芎嗪灌胃给药后,大鼠比目鱼肌肌肉收缩功能的变化。
2、利用定磷法测定不同浓度川芎嗪给药后,吊尾大鼠比目鱼肌肌浆网Ca2+-ATP酶活性。
研究结果：
1、与吊尾组比较：1)不同浓度川芎嗪灌胃给药组中,大鼠比目鱼肌的等长、强直收缩最大张力值均有显著增加,其中高浓度组等长收缩最大张力值增长179.02%,强直收缩最大张力值增长90.29%,低浓度组等长收缩最大张力值增长186.65%,强直收缩最大张力值增长123.61%。高低浓度之间无显著差异；2)不同浓度川芎嗪组收缩时程的收缩相TP50、TPT参数均显著增加,高浓度组分别增长148.09%和80.28%,低浓度组分别增长95.38%和31.67%,高低浓度之间无显著差异。3)高浓度川芎嗪组收缩时程的舒张相RT50参数增加21.36%(P<0.05)。
2、与吊尾组比较,灌胃给药川芎嗪后,高浓度组大鼠比目鱼肌肌浆网Ca2+-ATP酶活性升高55.60%(P<0.01),低浓度组升高71.84%(P<0.01)；不同浓度实验组中,低浓度组效果稍明显,但不具统计意义(P>0.05)。
研究结论：
1、不同浓度川芎嗪均能有效缓解吊尾造成的肌萎缩影响；有效对抗吊尾引发的比目鱼肌质网Ca2+-ATP酶活性降低。
2、川芎嗪可通过提高肌浆网Ca2+-ATP酶活性、抑制肌纤维类型转换等途径,对抗废用对肌肉收缩功能的影响。
Objectives:
1, To observe the effects of different concentrations of TMP on the soleus's contratility and on the sarcoplasmic reticulum Ca2+-ATPase activity in the tail-suspended rat.
2, To discuss the possible mechanism of TMP's effects on the soleus's contractility in rat
Methods:
1, The disuse-atrophy model was achieved by tail-suspension on rat; using PowerLab system and electronic stimulator (SEN.3301) to testify the soleus's contractility in rat.
2, The sarcoplasmic reticulum Ca2+-ATPase activity was measured by the Phosphate Determination method.
Results:
1, Compared with the tail-suspended group:1) both the peak twitch tension (Pt) and peak tetanic tension (Po) of soleus in rat have increased significantly in those groups of intragastric administration with different TMP concentrations; the Pt and P0 have increased 179.02%, 90.29% respectively in the high TMP concentration group, and have increased 186.65%, 123.61% respectively in the low TMP concentration group; there was no significant difference between the high and low TMP concentration groups.2) the time to 50% peak tension (TP50) and time to peak tension (TPT) have incerased notably in those groups of intragastric administration with different TMP concentrations; the TP50 and TPT have rised 148.09% and 80.28% respectively in the high TMP concentration group, and have increased 95.38% and 31.67% respectively in the low TMP concentration group; no significant changes was observed between the high and low TMP concentration groups.3) time from peak tension to 50% relaxation (RTso) has increased 21.36%(P<0.05) in the high TMP concentration group.
2, Compared with the tail-suspended group, after intragastric administration with TMP, the sarcoplasmic reticulum Ca2+-ATPase activity of the rat's soleus has increased 55.60%(P <0.01) and 71.84%(P<0.01) in high and low TMP concentration groups respectively; low TMP concentration was more effective than high one on the sarcoplasmic reticulum Ca2+-ATPase activity but was not statistically significant (P>0.05).
Conclusions:
1, Different concentrations of TMP can effectively alleviate the Tail on the impact caused by muscle atrophy; effective against the Tail on the soleus muscle induced endoplasmic reticulum Ca2+-ATP activity decreased.
2, TMP can increase sarcoplasmic reticulum Ca2+-ATP activity, inhibition of muscle fiber type conversion way, against the disuse of the muscle contraction function.
引文
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