香砂平胃散对胃肠运动影响的实验研究
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摘要
前言
功能性消化不良(Functional dyspepsia FD)是消化系统的常见疾病，在我国传统医学中，许多中药通过调理脾胃等作用，在治疗功能性消化不良方面取得了很好的疗效。香砂平胃散是由平胃散加木香、砂仁而成，主治因湿阻脾胃、运化失常所致的痞满、胃痛、呕吐、泄泻等病症，临床上常用于治疗各种慢性胃炎、肠炎、肠易激综合症等胃肠道疾患。但就香砂平胃散对胃肠运动影响的研究尚未见报道。我们以大分子色素葡聚糖蓝(BD)2000为标记物，以胃内色素残留量和小肠推进比为两项实验指标，采用正交设计方法，进行各因素各水平的比较，分析交互作用，并分析了几味中药的单独效应。
材料
1．研究对象：昆明小鼠，雄性，5周龄，体重18-22克，280只。
2．中药：选用苍术(河北)12克，厚朴(四川)9克，木香(云南)9克，陈皮(四川)9克，砂仁(广东)6克，甘草(内蒙古)6克(购于沈阳市药材采购供应站)，将其混合即香砂平胃散组方。制成煎剂浓度为0.51g／ml(各单味药的浓度分别为0.12g／ml，0.09g／ml，0.09g／ml，0.09g／ml，0.06g／ml，0.06g／ml)。
3．标记物：葡聚糖蓝2000(Blue Dextran2000，BD2000)(瑞典Phamacira公司生产)，以蒸馏水配成2％BD2000溶液。
4．西沙必利片剂[西安杨森制药有限公司，批号(96)卫药准
字－J－16O）号卜以蒸馏水配置为0 15mg／Inl的溶液。
5．仪器：75型分光光度计（上海分析仪器厂生产人
方 法
二．香砂平胃散对正常小鼠的胃肠运动的影响
将小鼠随机分组，每组20只，分成正常对照组、西沙必利组。
香砂平胃散组，各组每日分别给予蒸馏水、西沙必利溶液、香砂平
胃散煎剂各0．4Inl灌胃，共喂养二周，以 2％BD2000溶液为标记
物，测定灌胃后20分钟时的小肠推进比及胃内色素残留量。
2．各单味药物对小鼠胃肠运动的影响
将小鼠随机分组，每组10只，分别为对照组、香砂平胃散组。
苍术组、厚朴组、陈皮组、甘草组、木香组、砂仁组，分别用香砂平胃
散及各单味药进行实验，所用剂量仍为各药在复方药中的含量。
3．各单味药物对小鼠胃肠运动的影响及其交互作用
按正交设计表L；／2‘勺的安排采用主效应不与交互作用混杂
的正交表设计，即进行16组实验，2个水平，15个因子K个因子
为主效应列，7个两两混杂交互作用列，2个空白列人每味药为1
个因子，共6个因子为主效应列。每味药均取“不给药”和“给全
药量”两个水平。根据正交表中主效应列的水平配成16种不同
组合的配方。如第1组6个主效应列均为1水平，配成不含药物
的溶剂；第16组6个主效应列均为2水平，配成含6种药物的全
方剂。不管各组用药的成分有何不同，某一成分的用量固定不变，
而投药的容量也固定不变。将小鼠分16次进行实验、每组10只。
结 果
1．香砂平胃散对小鼠的胃肠运动功能的影响
·2·
香砂平胃散组及西沙必利组的胃残留率及小肠推进比与正常
对照组比较差异有显著性汐＜o．05人而上述埔间胃残留率及
小肠推进比无显著性差异瞩＞o．05人
2．各味药物对小鼠胃排空功能的影响及其交互作用
直观分析表明各单昧药增加胃排空效应的大小为：苍术＞砂
仁＞木香＞陈皮，厚朴和甘草则作用相反。方差分析结果提示苍
术、陈皮、木香、砂仁对胃排空有促进作用，厚朴、甘草作用不明显。
苍术与木香、苍术与陈皮、厚朴与甘草、甘草与砂仁有显著的协同
作用，但苍术与甘草＼厚朴与木香、陈皮与砂仁的交互作用为负效
应。
3．各味药物对小鼠小肠推进功能的影响及其交互作用
直观分析表明各单味药增加小肠推进比效应的大小为：木香
＞厚朴＞陈皮＞砂仁＞甘草，苍术作用则相反。方差分析提示木
香、厚朴、陈皮、砂仁可增加小肠推进比，甘草作用不明显，苍术则
抑制小肠运动功能。苍术与厚朴、苍术与陈皮、苍术与甘草、苍术
与木香、厚朴与甘草、厚朴与木香、陈皮与甘草、陈皮与砂仁、甘草
与砂仁均有协同作用。
4．各单昧药对小鼠胃肠运动功能的影响
除甘草组外，其余各组的胃残留率均明显低于对照组（P＜O．
of人苍术组、陈皮组和甘草组的胃残留率低于香砂平胃散组汐
＜o．05八厚朴组、木香组和砂仁组的胃残留率与香砂平胃散组比
较无显著性差异K＞o．05）。香砂平胃散组、木香组和砂仁组的
小肠推进比显著高于对照组汀＜o．01入苍术组、厚朴组的小肠推
进比高于对照组（P＜o．05厂陈皮组、甘草组与对照组比较无显著
性差异沙＞0．05八苍术组、厚朴组、陈皮组、甘草组和砂仁组的
小肠推进比低于香砂平胃散组（P＜o．05八木香组与香砂平胃散
组的小肠推进比无显著性差异（P＞o．05人
·3·
讨 论
香砂平胃散是由平胃散加木香、砂仁而成，临床上常用于治疗
各种胃肠道疾患。平胃散是《太平惠民和剂局方》中一传统方剂，
它具有燥湿健脾，
Preface
Functional dyspepsia (FD) is a common syndrome. Some of the patients with FD present symptoms suggestive of delayed gastric emptying. So gastrointestinal hypomotility is considered one of the pstho-genesis. In this study, we analysis the effect of Chinese herbs Xiang Sha Ping Wei San ( XSPWS) decoction and its component herbs on gastric emptying and small intestinal propulsion motility of big molecule chromogen Blue Dextron ( BD) 2000 in the experimental animals, and analysis the degree of role of each component herb in the compound herb, and the mutual roles of them.
Materials
1. Experimental animals,; 280 5 - week - old male Kunming mice.
2. Chinese herbs: Atractylodes rhizome 12g, Magnoliae officina-lis 9g, Tangerine peel 9g, Radix glycyrrhizae preparta 6g, Radix aucklandiae 9g, Fructus amomi 6g. XSPWS was made of the 6 Chinese herbs, and decocted into decoction which concentration was 0. 51g/ml (the concentration of every component herb was 0. 12g/ml, 0.09g/ml, 0.09g/ml, 0.06g/ml, 0.09g/ml, 0.06g/ml).
3. Maker; big molecule chromogen Blue Dextron (BD) 2000, compounded into 2% BD2000 solution with distilled water.
4. Cisapride was compounded into 0.15mg/ml cisapride solution
with distilled water.
5. 751G spectropbolometer .
Methods
Mice were randomly allocated to control group, cisapride group, and XSPWS group, and then received distilled water, cisapride, XSPWS decoction respectively by oral everyday during a period of 2 weeks. All experimental animals fasted an overnight before study. We used big molecule chromogen Blue Dextron ( BD) 2000 as the gastrointestinal transporting marker to observe the dosage of chromogen remaining in stomach and small intestinal propulsion rate after 20 minutes giving mice chromogen markers by oral.
Mice were randomly allocated to control group, XSPWS group, atractylodes rhizome group, magnoliae officinalis group, tangerine peel group, radix glycyrrhizae preparta group, radix aucklandiae group and fructus amomi group, and then received corresponding decoctions which contained certain concentration Chinese herbs. The dosage of chromogen remaining in stomach and the small intestinal propulsion rate in mice were measured after a period of 2 weeks being given drugs by oral.
We used the method of orthogonal design list L16(215) to determine the degree of role of each component herb in the compound herb, and the mutual roles of them. There were 6 factors and each factor had 2 levels, level 1 standing for the concentration of herb being 0, level 2 being certain concentration of each herb. We used different decoctions based the level of main effect of the orthogonal design list L16-(215) to make 16 experiments.
Results
Compared with control group, the rates of gastric chromogen emptying in XSPWS group and cisapride group were all significantly increased and their small intestinal propulsion rates increased (P <0. 05). The gastric emptying rate and intestinal propulsion rate had no significant difference between XSPWS group and cisapride group ( P > 0.05).
Except radix glycyrrhizae preparta group, the gastric emptying rates of other component herbal groups were significantly higher than that of control group ( P < 0. 01 ) . Compared with control group, the small intestinal propulsion rates of atractylodes rhizome group, magno-liae officinalis group, radix aucklandiae group, and fructus amomi groups were increased significantly (P <0. 05). The intestinal propulsion rates of tangerine peel group and radix glycyrrhizae preparta group were no significant difference with that of control group.
Based on the analysis of orthogonal design, we could conclude that the gastric emptying rate of six component herbs increased successively; atractylodes rhizom, fructus amomi, radix aucklandiae and tangerine peel. Magnoliae officinalis and radix glycyrrhizae preparta could not increase the rate of gastric emptying. There were wide ranging first grade mutual roles within the six component herbs. Between magnoliae officinalis and radix glycyrrh
功能性消化不良(Functional dyspepsia FD)是消化系统的常见疾病，在我国传统医学中，许多中药通过调理脾胃等作用，在治疗功能性消化不良方面取得了很好的疗效。香砂平胃散是由平胃散加木香、砂仁而成，主治因湿阻脾胃、运化失常所致的痞满、胃痛、呕吐、泄泻等病症，临床上常用于治疗各种慢性胃炎、肠炎、肠易激综合症等胃肠道疾患。但就香砂平胃散对胃肠运动影响的研究尚未见报道。我们以大分子色素葡聚糖蓝(BD)2000为标记物，以胃内色素残留量和小肠推进比为两项实验指标，采用正交设计方法，进行各因素各水平的比较，分析交互作用，并分析了几味中药的单独效应。
材料
1．研究对象：昆明小鼠，雄性，5周龄，体重18-22克，280只。
2．中药：选用苍术(河北)12克，厚朴(四川)9克，木香(云南)9克，陈皮(四川)9克，砂仁(广东)6克，甘草(内蒙古)6克(购于沈阳市药材采购供应站)，将其混合即香砂平胃散组方。制成煎剂浓度为0.51g／ml(各单味药的浓度分别为0.12g／ml，0.09g／ml，0.09g／ml，0.09g／ml，0.06g／ml，0.06g／ml)。
3．标记物：葡聚糖蓝2000(Blue Dextran2000，BD2000)(瑞典Phamacira公司生产)，以蒸馏水配成2％BD2000溶液。
4．西沙必利片剂[西安杨森制药有限公司，批号(96)卫药准
字－J－16O）号卜以蒸馏水配置为0 15mg／Inl的溶液。
5．仪器：75型分光光度计（上海分析仪器厂生产人
方 法
二．香砂平胃散对正常小鼠的胃肠运动的影响
将小鼠随机分组，每组20只，分成正常对照组、西沙必利组。
香砂平胃散组，各组每日分别给予蒸馏水、西沙必利溶液、香砂平
胃散煎剂各0．4Inl灌胃，共喂养二周，以 2％BD2000溶液为标记
物，测定灌胃后20分钟时的小肠推进比及胃内色素残留量。
2．各单味药物对小鼠胃肠运动的影响
将小鼠随机分组，每组10只，分别为对照组、香砂平胃散组。
苍术组、厚朴组、陈皮组、甘草组、木香组、砂仁组，分别用香砂平胃
散及各单味药进行实验，所用剂量仍为各药在复方药中的含量。
3．各单味药物对小鼠胃肠运动的影响及其交互作用
按正交设计表L；／2‘勺的安排采用主效应不与交互作用混杂
的正交表设计，即进行16组实验，2个水平，15个因子K个因子
为主效应列，7个两两混杂交互作用列，2个空白列人每味药为1
个因子，共6个因子为主效应列。每味药均取“不给药”和“给全
药量”两个水平。根据正交表中主效应列的水平配成16种不同
组合的配方。如第1组6个主效应列均为1水平，配成不含药物
的溶剂；第16组6个主效应列均为2水平，配成含6种药物的全
方剂。不管各组用药的成分有何不同，某一成分的用量固定不变，
而投药的容量也固定不变。将小鼠分16次进行实验、每组10只。
结 果
1．香砂平胃散对小鼠的胃肠运动功能的影响
·2·
香砂平胃散组及西沙必利组的胃残留率及小肠推进比与正常
对照组比较差异有显著性汐＜o．05人而上述埔间胃残留率及
小肠推进比无显著性差异瞩＞o．05人
2．各味药物对小鼠胃排空功能的影响及其交互作用
直观分析表明各单昧药增加胃排空效应的大小为：苍术＞砂
仁＞木香＞陈皮，厚朴和甘草则作用相反。方差分析结果提示苍
术、陈皮、木香、砂仁对胃排空有促进作用，厚朴、甘草作用不明显。
苍术与木香、苍术与陈皮、厚朴与甘草、甘草与砂仁有显著的协同
作用，但苍术与甘草＼厚朴与木香、陈皮与砂仁的交互作用为负效
应。
3．各味药物对小鼠小肠推进功能的影响及其交互作用
直观分析表明各单味药增加小肠推进比效应的大小为：木香
＞厚朴＞陈皮＞砂仁＞甘草，苍术作用则相反。方差分析提示木
香、厚朴、陈皮、砂仁可增加小肠推进比，甘草作用不明显，苍术则
抑制小肠运动功能。苍术与厚朴、苍术与陈皮、苍术与甘草、苍术
与木香、厚朴与甘草、厚朴与木香、陈皮与甘草、陈皮与砂仁、甘草
与砂仁均有协同作用。
4．各单昧药对小鼠胃肠运动功能的影响
除甘草组外，其余各组的胃残留率均明显低于对照组（P＜O．
of人苍术组、陈皮组和甘草组的胃残留率低于香砂平胃散组汐
＜o．05八厚朴组、木香组和砂仁组的胃残留率与香砂平胃散组比
较无显著性差异K＞o．05）。香砂平胃散组、木香组和砂仁组的
小肠推进比显著高于对照组汀＜o．01入苍术组、厚朴组的小肠推
进比高于对照组（P＜o．05厂陈皮组、甘草组与对照组比较无显著
性差异沙＞0．05八苍术组、厚朴组、陈皮组、甘草组和砂仁组的
小肠推进比低于香砂平胃散组（P＜o．05八木香组与香砂平胃散
组的小肠推进比无显著性差异（P＞o．05人
·3·
讨 论
香砂平胃散是由平胃散加木香、砂仁而成，临床上常用于治疗
各种胃肠道疾患。平胃散是《太平惠民和剂局方》中一传统方剂，
它具有燥湿健脾，
Preface
Functional dyspepsia (FD) is a common syndrome. Some of the patients with FD present symptoms suggestive of delayed gastric emptying. So gastrointestinal hypomotility is considered one of the pstho-genesis. In this study, we analysis the effect of Chinese herbs Xiang Sha Ping Wei San ( XSPWS) decoction and its component herbs on gastric emptying and small intestinal propulsion motility of big molecule chromogen Blue Dextron ( BD) 2000 in the experimental animals, and analysis the degree of role of each component herb in the compound herb, and the mutual roles of them.
Materials
1. Experimental animals,; 280 5 - week - old male Kunming mice.
2. Chinese herbs: Atractylodes rhizome 12g, Magnoliae officina-lis 9g, Tangerine peel 9g, Radix glycyrrhizae preparta 6g, Radix aucklandiae 9g, Fructus amomi 6g. XSPWS was made of the 6 Chinese herbs, and decocted into decoction which concentration was 0. 51g/ml (the concentration of every component herb was 0. 12g/ml, 0.09g/ml, 0.09g/ml, 0.06g/ml, 0.09g/ml, 0.06g/ml).
3. Maker; big molecule chromogen Blue Dextron (BD) 2000, compounded into 2% BD2000 solution with distilled water.
4. Cisapride was compounded into 0.15mg/ml cisapride solution
with distilled water.
5. 751G spectropbolometer .
Methods
Mice were randomly allocated to control group, cisapride group, and XSPWS group, and then received distilled water, cisapride, XSPWS decoction respectively by oral everyday during a period of 2 weeks. All experimental animals fasted an overnight before study. We used big molecule chromogen Blue Dextron ( BD) 2000 as the gastrointestinal transporting marker to observe the dosage of chromogen remaining in stomach and small intestinal propulsion rate after 20 minutes giving mice chromogen markers by oral.
Mice were randomly allocated to control group, XSPWS group, atractylodes rhizome group, magnoliae officinalis group, tangerine peel group, radix glycyrrhizae preparta group, radix aucklandiae group and fructus amomi group, and then received corresponding decoctions which contained certain concentration Chinese herbs. The dosage of chromogen remaining in stomach and the small intestinal propulsion rate in mice were measured after a period of 2 weeks being given drugs by oral.
We used the method of orthogonal design list L16(215) to determine the degree of role of each component herb in the compound herb, and the mutual roles of them. There were 6 factors and each factor had 2 levels, level 1 standing for the concentration of herb being 0, level 2 being certain concentration of each herb. We used different decoctions based the level of main effect of the orthogonal design list L16-(215) to make 16 experiments.
Results
Compared with control group, the rates of gastric chromogen emptying in XSPWS group and cisapride group were all significantly increased and their small intestinal propulsion rates increased (P <0. 05). The gastric emptying rate and intestinal propulsion rate had no significant difference between XSPWS group and cisapride group ( P > 0.05).
Except radix glycyrrhizae preparta group, the gastric emptying rates of other component herbal groups were significantly higher than that of control group ( P < 0. 01 ) . Compared with control group, the small intestinal propulsion rates of atractylodes rhizome group, magno-liae officinalis group, radix aucklandiae group, and fructus amomi groups were increased significantly (P <0. 05). The intestinal propulsion rates of tangerine peel group and radix glycyrrhizae preparta group were no significant difference with that of control group.
Based on the analysis of orthogonal design, we could conclude that the gastric emptying rate of six component herbs increased successively; atractylodes rhizom, fructus amomi, radix aucklandiae and tangerine peel. Magnoliae officinalis and radix glycyrrhizae preparta could not increase the rate of gastric emptying. There were wide ranging first grade mutual roles within the six component herbs. Between magnoliae officinalis and radix glycyrrh
引文
1．柯美云．功能性消化不良的研究动向．新消化病学杂志，1996；4(11)：601-602
2．窦艳玲，柯美云．功能性消化不良．华人消化杂志，1998；6(2)：165-167
3．朱金照，杨国汉，冷恩仁，陈东风．中药的促胃肠动力作用．世界华人消化杂志，1999；7：689-690
4．马晓松，樊雪品萍，陈忠，李崇文，邢诒善．白术对离体豚鼠回肠收缩的影响．新消化病学杂志，1996；4：603-604
5．王振华，吕立生．中药胃肠通治疗胃肠功能紊乱．新消化病学杂志，1997；5：448-449
6．李岩，孙思予，周卓，陈苏宁，王兴尧．健脾行气及消食中药组合对小鼠胃肠动力的影响．华人消化杂志，1998；6(特刊7)：208-209
7．郝庆，李岩，尹洪涛．不同品种柴胡枳实对小鼠胃肠动力作用的比较研究。华人消化杂志．，1998；6(特刊7)：205-207
8．冯卫红，侯家玉．沙参麦冬汤对动物在体胃运动的影响．中国中西医结合杂志，1996；16：164-165
9．吴皓，蔡宝昌，荣根新，叶定江．半夏姜制对动物胃肠道功能的影响．中国中药杂志，1994：19：535-537
10．李胜利，郭宏春，张梅．中西药合用治疗胃节律紊乱综合症临床观察．中国中西医结合脾胃杂志，1995；3：87-88
11．朱金照，冷恩仁，陈东风等．15味中药促胃肠动力作用的筛选研究．第三军医大学学报。2000，22(5)：436-438
12．陈少夫，李宇权，何凤云，潘丽丽．木香对胃酸分泌、胃排空及血清胃泌素、生长抑素、胃动素水平的影响．中国中西医结合杂志，1994；14：406-408
13．李岩，孙思宇，周卓．消食行气中药对小鼠胃肠动力的影响．新消化病学杂志，1997，5(3)：153
14．苗明三主编《实验动物和实验动物技术》．中国中医药出版社，1997年6月；142-145
15．段丽平，郑芝田．中华消化杂志，1992；12(2)66-69
16．李增烈，廖宁逊．我国功能性消化不良临床类型概况及治疗回顾的研究．中华消化杂志，1997；17：13-15
17. Labo Etal Interdigestine gastroduodenal motility and serum motilin levels in patients with idiopathic delay in gastric emptying. Gastroenterology, 1986;90(1):20-26
18．赵荣莱．功能性消化不良的治疗进展。华人消化杂志，1998；6(4)：340-341
19. Haarst AD, Klooster G, Gerven JM. The influence of cisapride and clarithromycin on QT intervals in healthy volunteers. Clin Pharmacol Ther, 1998 Nov;64(5):542-6
20．郭金龙，颜正华，周吕．不换金正气散芳香化湿醒脾的实验研究．中国医药学报，1989；4(4)：265-268
21．郑天珍，李伟，翟颂义．理气类中药对大鼠离体平滑肌条的作用．兰州医学源院学报，1998，24(4)：1-3
22．郑天珍，李伟，翟颂义．补气类中药对大鼠离体平滑肌条的作用．兰州医学源院学报，1998，24(3)：3-5
23．邱赛红，陈立峰，柳克玲等．芳香化湿药开胃作用机理的实验研究．中药药理与临床，1995；(4)：24-26
24．李伟，郑天珍，翟颂义．芳香化湿类中药对大鼠离体平滑肌条的作用．兰州医学院学报，1998，24(4)：6-8
25．李岩，陈苏宁，田代真一．葡聚糖蓝2000标记五积散对鼠胃排空及小肠推进功能的影响。中国医科大学学报，1996；25：160-161。
2．窦艳玲，柯美云．功能性消化不良．华人消化杂志，1998；6(2)：165-167
3．朱金照，杨国汉，冷恩仁，陈东风．中药的促胃肠动力作用．世界华人消化杂志，1999；7：689-690
4．马晓松，樊雪品萍，陈忠，李崇文，邢诒善．白术对离体豚鼠回肠收缩的影响．新消化病学杂志，1996；4：603-604
5．王振华，吕立生．中药胃肠通治疗胃肠功能紊乱．新消化病学杂志，1997；5：448-449
6．李岩，孙思予，周卓，陈苏宁，王兴尧．健脾行气及消食中药组合对小鼠胃肠动力的影响．华人消化杂志，1998；6(特刊7)：208-209
7．郝庆，李岩，尹洪涛．不同品种柴胡枳实对小鼠胃肠动力作用的比较研究。华人消化杂志．，1998；6(特刊7)：205-207
8．冯卫红，侯家玉．沙参麦冬汤对动物在体胃运动的影响．中国中西医结合杂志，1996；16：164-165
9．吴皓，蔡宝昌，荣根新，叶定江．半夏姜制对动物胃肠道功能的影响．中国中药杂志，1994：19：535-537
10．李胜利，郭宏春，张梅．中西药合用治疗胃节律紊乱综合症临床观察．中国中西医结合脾胃杂志，1995；3：87-88
11．朱金照，冷恩仁，陈东风等．15味中药促胃肠动力作用的筛选研究．第三军医大学学报。2000，22(5)：436-438
12．陈少夫，李宇权，何凤云，潘丽丽．木香对胃酸分泌、胃排空及血清胃泌素、生长抑素、胃动素水平的影响．中国中西医结合杂志，1994；14：406-408
13．李岩，孙思宇，周卓．消食行气中药对小鼠胃肠动力的影响．新消化病学杂志，1997，5(3)：153
14．苗明三主编《实验动物和实验动物技术》．中国中医药出版社，1997年6月；142-145
15．段丽平，郑芝田．中华消化杂志，1992；12(2)66-69
16．李增烈，廖宁逊．我国功能性消化不良临床类型概况及治疗回顾的研究．中华消化杂志，1997；17：13-15
17. Labo Etal Interdigestine gastroduodenal motility and serum motilin levels in patients with idiopathic delay in gastric emptying. Gastroenterology, 1986;90(1):20-26
18．赵荣莱．功能性消化不良的治疗进展。华人消化杂志，1998；6(4)：340-341
19. Haarst AD, Klooster G, Gerven JM. The influence of cisapride and clarithromycin on QT intervals in healthy volunteers. Clin Pharmacol Ther, 1998 Nov;64(5):542-6
20．郭金龙，颜正华，周吕．不换金正气散芳香化湿醒脾的实验研究．中国医药学报，1989；4(4)：265-268
21．郑天珍，李伟，翟颂义．理气类中药对大鼠离体平滑肌条的作用．兰州医学源院学报，1998，24(4)：1-3
22．郑天珍，李伟，翟颂义．补气类中药对大鼠离体平滑肌条的作用．兰州医学源院学报，1998，24(3)：3-5
23．邱赛红，陈立峰，柳克玲等．芳香化湿药开胃作用机理的实验研究．中药药理与临床，1995；(4)：24-26
24．李伟，郑天珍，翟颂义．芳香化湿类中药对大鼠离体平滑肌条的作用．兰州医学院学报，1998，24(4)：6-8
25．李岩，陈苏宁，田代真一．葡聚糖蓝2000标记五积散对鼠胃排空及小肠推进功能的影响。中国医科大学学报，1996；25：160-161。