利用罗非鱼制备低聚肽的研究
|
摘要
随着我国罗非鱼产量的逐年增加,开展罗非鱼的增值加工显得日益迫切。本研究以罗非鱼肉的酶解物为原料,探讨利用树脂分离其酶解液中低聚肽的可行性,从而提高罗非鱼的附加值,并降低低聚肽的生产成本,以便于其产业化,满足人们对保健低聚肽产品的需求。主要结果如下:
(1)罗非鱼酶解工艺:底物浓度(煮熟鱼糜)5%,Alcalase酶添加量为2300μ/g.鱼蛋白,在pH=8.0,55℃下反应4h后,灭酶,离心(过滤),得上清液。在此条件下,可获得平均水解度为17.86%的酶解液。
(2)酶解液中游离氨基酸的脱除:采用阳离子交换树脂732分离酶解液中的游离氨基酸及低聚肽,具体工艺为:料液pH为3.0,室温下以流速1mL/min上柱;用1mol/L NaOH溶液洗脱,洗脱速度为1mL/min。残余蛋白(多肽)、氨基酸含量、平均水解度及离子色谱结果表明,阳离子交换树脂能有效吸附氨基酸,吸附率为82.16%,但对残余蛋白(多肽)吸附少,吸附率仅为36.30%,且平均水解度无显著变化;1mol/L NaOH溶液除能洗脱游离氨基酸外,还能洗脱碱性氨基酸比例大的小分子低聚肽,洗脱率为78.07%。
(3)低聚肽的分离:静态交换实验表明,大孔树脂AB-8能有效吸附低聚肽。通过紫外特征吸收、氨基氮含量、平均水解度、平均肽链长度变化、F值及离子色谱,确定其最佳柱层析工艺为:料液直接上柱,流速1mL/min;采用5%乙醇溶液洗脱,洗脱流速为1mL/min;在此条件下,可获得平均肽链长度分别为1.18～6.43的低聚肽。产物的紫外吸收图谱、离子色谱图和氨基酸自动分析结果表明,产物氨基酸种类较多,表明获得的低聚肽为多种低聚肽的混合物,需进一步分离纯化。
China is the largest country for Tilapia production and the output increases annually,and its value-added processing becomes more and more necessary.In this paper,preparation of oligopeptides from Tilapia enzymatic hydrolysate using resins was investigated,the main results were as follows:
(1)Preparation of enzymatic hydrolysate from Tilapia.The optimal technology is mixed of 5%cooked Tilapia homogenate with 2300u/g fish protein and reacted at pH 8.0 and 55℃for 4h, the undissolved solids in the slurries were removed by centrifugation.Under this enzymatic condition,the average degree of hydrolysis(DH) is 17.86%.
(2)Removal of free amino acids from the enzymatic hydrolysate.Amino acids were separated from the enzymatic hydrolysate using cation exchange resin 732.The adsorption condition of free amino acids by cation exchange chromatography was pH3.0 and the flow rate kept at 1mL/min;amino acids were desorbed with 1mol/L NaOH at elution rate 1mL/min.The changes of remaining protein(or polypeptides),amino acids content and average hydrolysis degree in the eluant showed that cation exchange resin 732 had high capacity for adsorbing free amino acids but not for peptide.The adsorption for free amino acids accounts for 82.16%but only 36.30%for peptides.However,there was no significance between the average hydrolysis degree changes.Apart from free amino acids,some small molecular peptides which have high percentage of alkaline amino acids were also eluted by 1mol/L NaOH.The elution rate was 78.07%.
(3)Seperation of oligopeptides.Static exchange experiment indicated that macroporous resin AB-8 effectively adsorbed oligopeptides from free amino acids-removed enzymatic hydrolysate. The condition for macroporous resin chromatography was optimized by analyzing the changes of oligopeptides ultraviolet adsorption,amino nitrogen content,average hydrolysis degree,average peptide chain length,fischer ratio and ion chromatogram.The purified enzymatic hydrolysate was passed through the column directly and the flow rate kept at 1mL/min;oligopeptides were eluted with 5%ethanol at 1mL/min.Oligopeptides with average peptide chain length from 1.18 to 6.43 were obtained.However,the ultraviolet spectrum and amino acid analysis of oligopeptides showed that the oligopeptide contains more than 6 kinds of amino acids,indicating that the products were not highly purified.
(1)罗非鱼酶解工艺:底物浓度(煮熟鱼糜)5%,Alcalase酶添加量为2300μ/g.鱼蛋白,在pH=8.0,55℃下反应4h后,灭酶,离心(过滤),得上清液。在此条件下,可获得平均水解度为17.86%的酶解液。
(2)酶解液中游离氨基酸的脱除:采用阳离子交换树脂732分离酶解液中的游离氨基酸及低聚肽,具体工艺为:料液pH为3.0,室温下以流速1mL/min上柱;用1mol/L NaOH溶液洗脱,洗脱速度为1mL/min。残余蛋白(多肽)、氨基酸含量、平均水解度及离子色谱结果表明,阳离子交换树脂能有效吸附氨基酸,吸附率为82.16%,但对残余蛋白(多肽)吸附少,吸附率仅为36.30%,且平均水解度无显著变化;1mol/L NaOH溶液除能洗脱游离氨基酸外,还能洗脱碱性氨基酸比例大的小分子低聚肽,洗脱率为78.07%。
(3)低聚肽的分离:静态交换实验表明,大孔树脂AB-8能有效吸附低聚肽。通过紫外特征吸收、氨基氮含量、平均水解度、平均肽链长度变化、F值及离子色谱,确定其最佳柱层析工艺为:料液直接上柱,流速1mL/min;采用5%乙醇溶液洗脱,洗脱流速为1mL/min;在此条件下,可获得平均肽链长度分别为1.18～6.43的低聚肽。产物的紫外吸收图谱、离子色谱图和氨基酸自动分析结果表明,产物氨基酸种类较多,表明获得的低聚肽为多种低聚肽的混合物,需进一步分离纯化。
China is the largest country for Tilapia production and the output increases annually,and its value-added processing becomes more and more necessary.In this paper,preparation of oligopeptides from Tilapia enzymatic hydrolysate using resins was investigated,the main results were as follows:
(1)Preparation of enzymatic hydrolysate from Tilapia.The optimal technology is mixed of 5%cooked Tilapia homogenate with 2300u/g fish protein and reacted at pH 8.0 and 55℃for 4h, the undissolved solids in the slurries were removed by centrifugation.Under this enzymatic condition,the average degree of hydrolysis(DH) is 17.86%.
(2)Removal of free amino acids from the enzymatic hydrolysate.Amino acids were separated from the enzymatic hydrolysate using cation exchange resin 732.The adsorption condition of free amino acids by cation exchange chromatography was pH3.0 and the flow rate kept at 1mL/min;amino acids were desorbed with 1mol/L NaOH at elution rate 1mL/min.The changes of remaining protein(or polypeptides),amino acids content and average hydrolysis degree in the eluant showed that cation exchange resin 732 had high capacity for adsorbing free amino acids but not for peptide.The adsorption for free amino acids accounts for 82.16%but only 36.30%for peptides.However,there was no significance between the average hydrolysis degree changes.Apart from free amino acids,some small molecular peptides which have high percentage of alkaline amino acids were also eluted by 1mol/L NaOH.The elution rate was 78.07%.
(3)Seperation of oligopeptides.Static exchange experiment indicated that macroporous resin AB-8 effectively adsorbed oligopeptides from free amino acids-removed enzymatic hydrolysate. The condition for macroporous resin chromatography was optimized by analyzing the changes of oligopeptides ultraviolet adsorption,amino nitrogen content,average hydrolysis degree,average peptide chain length,fischer ratio and ion chromatogram.The purified enzymatic hydrolysate was passed through the column directly and the flow rate kept at 1mL/min;oligopeptides were eluted with 5%ethanol at 1mL/min.Oligopeptides with average peptide chain length from 1.18 to 6.43 were obtained.However,the ultraviolet spectrum and amino acid analysis of oligopeptides showed that the oligopeptide contains more than 6 kinds of amino acids,indicating that the products were not highly purified.
引文
[1]刘石生,易美华,黄文艳.热水提取罗非鱼鱼鳞胶原蛋白的研究[J].现代食品科技,2006,22(4):139-141。
[2]孔美兰,吉宏武,章超桦.罗非鱼下脚料自溶条件的初步探讨[J].湛江海洋大学学报,2005,25(4):27-31.
[3]吴燕燕,李来好.罗非鱼片干的微波烘烤技术[J].渔业现代化,2002,(3):31-33.
[4]吴文龙.罗非鱼鱼丸的加工工艺研究[J].食品科技,2006,(2):39-41.
[5]邓后勤,夏延斌,曹小彦,危小湘,曹薇,徐发.用罗非鱼碎肉制作鱼松的生产工艺研究[[J].现代食品科技,2005,21(3):80-82.
[6]洪鹏志,杨萍,章超桦,吉宏武,刘书成,郝记明,张静.酶解罗非鱼下脚料制备的超微罗非鱼骨粉营养价值分析[J].食品与机械,2007,23(3):125-126,131.
[7]段振华,汪菊兰,王志国,易美华.罗非鱼的加工技术[J].科学养鱼,2006,1:69-70.
[8]段振华,尚军,徐松,王锡彬,易美华.罗非鱼的热风干燥特性及其主要成分含量变化研究[J].食品科学,2006,27(12):479-482.
[9]刁石强,李来好,杨贤庆,吴燕燕,徐泽智.冻罗非鱼片加工技术工艺研究[J].制冷,2005,24(3):6-10.
[10]粟桂娇,阎欲晓,申柯,覃子海.酶法制取罗非鱼水解动物蛋白的工艺研究[J].食品科学,2005,26(4):177-182.
[11]朱志伟,曾庆孝,林奕封,张立彦,阮征.采用控制酶解法从罗非鱼肉中制备血管紧张素转化酶抑制肽[J].食品与发酵工业,2004,30(12):71-76.
[12]吴燕燕,李来好,岑剑伟,周婉君,杨贤庆,陈胜军,刁石强.酶法由罗非鱼加工废弃物制取调味料的研究[J].南方水产,2006,2(1):49-53.
[13]吴林泽,李从发.罗非鱼下脚料酶解产物中抗菌肽的初步研究[J].科技信息(科学教研),2007,(17):283,428.
[14]丁卓平,刘振华,李仁冬,朱卫钦.罗非鱼鱼皮胶原蛋白提取条件的研究[J].水产科技情报,2006,33(4):190-192.
[15]陈胜军,李来好,曾名勇,杨贤庆,吴燕燕,刁石强。罗非鱼鱼皮胶原蛋白降血压酶解液的制备与活性研究[J].食品科学,2005,26(8):229-233.
[16]李小勇,李洪军.高F值低聚肽研究进展[J].粮食与油脂,2006,(6):9-11.
[17]易维学,胡顺涛,娄宇.大豆低聚肽的开发与应用[J].精细与专用化学品,2001,(1):15-17.
[18]Yamakawa M.Peptide digestion and absorption in humans:portal vein hepatic vein,and peripheral venous amino acid concentration[J].Asia Pacific Journal of Clinical Nutrition,1997,(6):88-91.
[19]曹文红,章超桦,秦小明.食品蛋白来源生物活性肽[J].粮食与油脂,2007,(2):3-6.
[20]Jolles P,Parker F,Migliore D.Immunostimulating substances from human casein[J].Journal of Immumnopharmacology,1981,(3):363-369.
[21]王梅,沈辉.食物蛋白酶解物中的生物活性肽[J].氨基酸和生物资源,1997,19(1):40-43.
[22]王秋韫,庞广昌,陈庆森.免疫活性肽的研究进展与展望[J].食品科学,2002,23(7):136-139.
[23]钟英英.天然免疫调节肽的研究概况[J].重庆科技学院学报(自然科学版),2005,7(2):69-72.
[24]阚建全主编.食品化学[M].北京:中国农业大学出版社,2002.154-156.
[25]杨金良,李文平,王克诚等.促吞噬肽抗肿瘤作用的研究[J].中国医药杂志,2000,35(1):23-25.
[26]黄艳春.酶解淡水鱼蛋白制取血管紧张素转化酶抑制肽的研究[D].武汉:华中农业大学硕士学位论文,2004.4,14,16,28-29.
[27]Ferreira S H.A bradykinin-potentiating factor(BPF) present in the venom of Bathrops jararaca[J].British Journal of Pharmacology,1965,24:163-165.
[28]Li G H,Le G W,Shi Y H,Shrestha S.Angiotensin Ⅰ-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects[J].Nutrtion Research,2004,27:469-486.
[29]安桂香,庄桂东,徐振凯,姚明经,迟玉森.食物中血管紧张素转化酶抑制肽的研究进展[J].食品研究与开发,2006,27(6):173-175.
[30]Kohmura M,Nio N,Ariyosh Y.Inhibition of angiotensin-converting enzyme by synthetic peptide fragments of human-casein[J].Agricultural and Biological Chemistry,1990,54:835-836.
[31]Fujita H,Yokayama K,Yoshikawa M.Classification and antihypertensive activity of angiotensin Ⅰ-converting enzyme inhibitory peptides derived from food proteins[J].Journal of Food Science,2000,65:564-569.
[32]卢燕婉,李作孝,荣本兵.神经肽和血管性痴呆[J].医学综述,2006,12(6):348-350.
[33]刘伟.血管加压素的研究进展[J].国外医学神经病学神经外科学分册,2002,29(1):38-41.
[34]Bicego-Nahas K C,Steiner A A,Carnio E C.Antipyretic effect of arginine vasotocin in toads[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2000,278(6):1408-1414.
[35]雷治海.家畜中枢神经系统神经肽的分布定位研究进展[J].畜牧与兽医,2002,24(4):39-42.
[36]Tirelli A,De Noni I,Resmini P.Bioactive peptides in milk products[J].Italy Journal of Food Science,1997,(2):91-98.
[37]Jolles P,Loucheux-Lefebvre M H,Henschen A.Structural relatedness of κ-casein and fibrinogen γ-chain[J].Journal of Molecular Evolution,1978,(11):271-277.
[38]Fiat A M,Migliore-Samour D,Holies P.Biologically active peptides from milk proteins with emphasis on two samples concerning antitrombotic and immunomodulating activities[J].Journal of Dairy Science,1993,76:301-310.
[39]赵利,苏伟,胡火根,戴银银,杨玉玲.胶原蛋白生物活性肽的研究进展[J].食品科学,2005,26(9):578-582.
[40]Nonaka I,Katsuda S,Ohmori T.In vitro and in vivo anti-platelet effects of enzymatic hydrolysates of collagen and collagen-related peptides[J].Bioscience Biotechnology and Biochemistry,1997,61(5):712-725.
[41]Saiga A,Tanabe S,Nishimura T.Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment[J].Journal of Agricultural and Food Chemistry,2003,51:3661-3667.
[42]Hattori M,Yamaji-Tukamoto K,Kumagai H.Antioxidative activity of soluble elastin peptides[J].Journal of Agricultural and Food Chemistry,1998,46:2167-2170.
[43]Kim S K,Kim Y T,Byun H G.Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin[J].Journal of Agricultural and Food Chemistry,2001,49:1984-1989.
[44]Chen H M,Muramoto K,Yamauchi F.Antioxidative properties of histidine-containing peptides desigened from peptide fragments found in the digests of a soybean protein[J].Journal of Agriculture and Food Chemistry,1998,46:49-53.
[45]Suetsuna K,Ukeda H,Ochi H.Isolation and characterization of free radical scavenging activities peptides derived from casein[J].Journal of Nutritional Biochemistry,2000,11:128-131.
[46]彭德翔,丁卓平.抗衰老活性肽的研究进展[J].现代食品科技,2006,22(2):267-270.
[47]McFarland G A,Holliday R.Retardation of the senescence of cultured human diploid fibroblasts by camosine[J].Experimental Cell Research,1994,212:167-175.
[48]McFarland G A,Holliday R.Further evidence for the rejuvenating effects of the dipeptide L-carnosine on cultured human diploid fibroblasts[J].Experimental Gernotology,1999,34:35-45.
[49]Fischer J E.The utilization of BCAA in the treatment of hepatic coma[A].Capocaccia L,Fischer J E,Rossi-Fanelli Feds.Hepatic encephalopathy in chronic liver failure[C].New York:Plenum,1984.11-12.
[50]Chan D I,Prenner E J,Vogel H J.Tryptophan- and arginine-rich antimicrobial peptides:Structures and mechanisms of action[J].Biochimica et Biophysica Acta,2006,1758:1184-1202.
[51]Brantl V,Teschemacher H.A material with opioid activity in bovine milk and milk products[J].Naunyn-Schmeidebergs Arch Pharmacology,1979,306:301-304.
[52]Zioudrou C,Streaty R A,Klee W A.Opioid peptides derived from food proteins[J].Journal of Biology Chemistry,1979,254:2446-2449.
[53]Meisel H.Overview on milk protein-derived peptides[J].International Dairy Journal,1998,8(6):363-373.
[54]聂剑初,吴国利,张翼伸,杨绍钟,刘鸿铭合编.生物化学简明教程[M].北京:高等教育出版社,1999.15-16.
[55]舒鼎铭,覃健萍,曹永长.缩胆囊素(CCK)的生物学功能研究进展[J].饲料工业,2004,25(11):12-16.
[56]陈大庆,倪宏.缩胆囊素生物学作用的多样性[J].国外医学儿科学分册,2002,29(3):164-166
[57]Crawley J N,Corwin R L.Biological actions of cholecystokinin[J].Peptides,1994,15(4): 731-755.
[58]Nomoto S,Miyake M,Ohta M.Impaired learning and memory in OLETF rats without cholecystokinin(CCK)-A receptor[J].Physiology and Behavior,1999,66(5):869-872.
[59]Rezayat M,Ravandeh N,Zarrindast M R.Cholecystokinin and morphine-induced hypothermia[J].European Neuropsychopharmacology,1999,9(3):219-225.
[60]姜杰,熊忠,胡健饶.神经肽与学习记忆[J].生物学通报,2002,37(3):12-14.
[61]董华强,刘本国,吴琼,曹劲松,宁正祥.几种新海洋动物活性肽研究概述[J].食品科学,2005,26(9):570-573.
[62]McWilliams D F,Watson S A,Crosbee D M.Coexpression of gastrin and gastrin receptors (CCK-B and delta CCK-B) in gastrointestinal tumour cell lines[J].Gut,1998,42:795-798.
[63]Smith J P,Shin A,Wu Y.Gastrin regulates growth of human pancreatic cancer in a tonic and autocrine fashion[J].American Journal of Physiology,1996,270:1078-1084.
[64]Caplin M E,Khan K,Grimes S.Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines[J].Digestive Diseases and Sciences,2001,46:1356-1366.
[65]方富贵,章孝荣.P物质研究进展[J].动物医学进展,2005,26(1):6-8.
[66]包新民,舒斯云,包蓉,张魁华.P物质和受体在纹状体边缘区内的分布及其与学习记忆功能的关系[J].第一军医大学学报,2002,22(2):102-105.
[67]邓云川,李世荣,丛林.P物质及其受体对成纤维细胞的作用[J].实用美容整形外科杂志,2002,13(6):317-319.
[68]岑怡红,方俊,李宗军,孟德连,邓近平.制备活性肽的主要方法研究[J].现代农业科技,2006,(12):151,153.
[69]Ondetti M A,Rubin B,Cushman D W.Design of specific inhibitors of angiotensin-converting enzyme:New class of orally active antihypertensive agents[J].Science,1977,196:441-444.
[70]黎观红.食物蛋白源血管紧张素转化酶抑制肽的研究[D].无锡:江南大学博士学位论文,2005.1,5,10.
[71]王利亚,徐建荣,吴复初,姚毅敏,彭清洋.新一代甜味剂天苯二肽的合成方法及应用前景[J].江苏化工,1999,27(2):9-12.
[72]梁汉萦,刘成梅,刘伟.食源性ACE抑制肽的研究进展[J].食品研究与开发,2007,28(8):156-158.
[73]Korhonen H,Pihlanto A.Bioactive peptides:Production and functionality[J].International Dairy Journal,2006,16:945-960.
[74]Ravallec-Ple R,Wormhoudt A.Secretagogue activities in cod(Gadus morhua) and Shrimp (Penaeus aztecus) extracts and alcalase hydrolysates determined in AR4-2J pancreatic tumour cells[J].Comparative Biochemistry and Physiology Part B,2003,134:669-679.
[75]Kim Y E,Yoon S,Yu D Y,Lonnerdal B.Chung B H.Novel angiotensin-Ⅰ-converting enzyme inhibitory peptides derived from recombinant human α_(sl)-casein expressed in Escherichia coli[J].Journal of Dairy Research,1999,66:431-439.
[76]范长胜.氨基酸二肽甜味剂的开发研究进展[J].工业微生物,2002,32(2):37-40,47.
[77]汪江波.发酵工业中的膜分离技术[J].中国酿造,1999,(6):1-3.
[78]Mulder M.Basic Principles of Membrane Technology[M].Netherlands:Kluwer Academic publish.1996:1.
[79]霍建新.膜分离法的应用[J].安徽农学通报,2007,13(14):41-42.
[80]Righetti P G,Nembri F,Bossi A,Mortarino M.Continuous enzymatic hydrolysis of beta-casein and isoelectric collection of some of the biologically active peptides in an electric field[J].Biotechnology Progress,1997,13:258-264.
[81]Turgeon S L,Gauthier S F.Whey peptide fractions obtained with a two-step ultrafiltration process:Production and characterization[J].Journal of Food Science,1990,55:106-110.
[82]Pihlanto-Leppala A,Koskinen P,Paakkari I,Tupasela T,Korhonen H.Opioid whey protein peptides obtained by membrane filtration[J].IDF Bulletin,1996,311:36-38.
[83]Pihlanto-Leppala A,Koskinen P,Piilola K,Tupasela T,Korhonen H.Angiotensin Ⅰ-converting enzyme inhibitory properties of whey protein digests:Concentration and characterization of active peptides[J].Journal of Dairy Research,2000,67:53-64.
[84]尚庆坤,祝红,阎吉昌,向前.高效制备液相色谱法分离制备虻虫中的多肽样品[J].分析化学,1999,27(8):924-926.
[85]白泉,葛小娟,耿信笃.反相液相色谱对多肽的分离、纯化与制备[J].分析化学,2002,30(9):1126-1129.
[86]朱晓囡,苏志国.反相液相色谱在蛋白质及多肽分离分析中的应用[J].分析化学,2004,32(2):248-254.
[87]张玉奎.现代生物样品分离分析方法[M].北京:科学出版社,2003.18-19.
[88]Fulton S P,Afeyan N B,Gordon N F.Very high speed separations of proteins with a 20μm reverse-phase sorbent[J].Journal of Chromatography,1991,452-547.
[89]钟芳,张晓梅,麻建国.大豆肽的离子交换色谱分离及其活性评价[J].食品与机械,2006,22(5):16-19.
[90]张晓峰,陈庆森,庞广昌.液相色谱在多肽物质分离分析上的应用[J].食品科学,2006,27(3):239-241.
[91]Application Note No.Separation of Tryptophan and Methionine Oxidized Peptides from Their Unoxidized Forms,129 Dionex Corporation,Sunnyvale,CA,2002,5.
[92]钟芳,张晓梅,麻建国.大豆肽的大孔吸附树脂以及凝胶过滤色谱分离[J].食品与机械,2006,22(4):25-28,45.
[93]田益玲,陈冠华,祝彦忠.高效毛细管电泳在食品添加剂中应用的研究进展[J].河北农业大学学报,2002,25:227-229.
[94]李卫红,徐宁,杨宝刚,丘竹贤.电泳研究及应用的进展[J].有色矿冶,1998,(3):18-21.
[95]Jorgenson J W,Lukacs K D.Zone electrophoresis in open-tubular class capillaries[J].Analytical Chemistry,1981,53(8):1298-1302.
[96]舒友琴,罗国安.毛细管电泳在食品分析中的应用[J].食品科学,1999,7:8-12.
[97]刘静,陈均志.大豆多肽分离纯化方法研究进展[J].食品工业科技,2006,27(9):171-174.
[98]刘展眉,熊绪杰,吕钱江,万岩雄.毛细管电泳及其在天然产物分离分析中的应用进展[J].仲凯农业技术学院学报,2002,15(3):59-65.
[99]冯淑波,陈萍.高效毛细管电泳的应用[J].保定师范专科学校学报,2006,19(2):31-33.
[100]孟祥平,刘建学.毛细管电泳在食品分析中的应用进展[J].食品与发酵工业,2005,31(11):88-92.
[101]Issaq H J,Janini G M,Atamana I Z,Muschik G M,Lukszo J.Capillary electrophoresis separation of small peptides,effect of pH buffer additives,and temperature[J].Journal of Liquid Chromatography,1992,15:1129-1142.
[102]Kristensen H K,Hansen S H.Separation of polymyxins by mieellar electrokinetie capillary chromatography[J].Journal of Chromatography,1993,628:309-315.
[103]Knox J H,Grant I H.Electrochromatography in packed tubes using 1.5 to 50μm silica gels and ODS bonded silica gels[J].Chromatographia,1991,32(7-8):317-328.
[104]王喜波,迟玉杰.活性炭色谱法分离芳香族氨基酸制备高F值蛋清低聚肽混合物的研究[J].食品工业科技,2005,26(6):55-57.
[105]Nokihara K.Procedures leading to primary structure determination of proteins in complex mixtures by gel electrophoresis and modern micro-scale analysis[J].Analytica Chimica Acta,1998,372(1-2):21-32.
[106]梁振江,纪明慧,李繁,黄澜.粘度法测定高聚物相对分子质量方法的改进[J].海南师范学院学报(自然科学版).2002,15(1):73-77.
[107]马永强,赵毅,石彦国.大豆蛋白水解物中肽分子分布的研究[J].中国粮油学报,2001,16(3):15-17.
[108]陈均志,张海平,顾玲,陈立红.高效毛细管电泳—紫外检测法分离测定微波复合酶法大豆蛋白水解物中的多肽[J].食品工业科技,2005,26(8):163-165.
[109]汤国营,戴军,蔡木易,易维学,谷瑞增.大豆低聚肽分子质量分布的测定及体外消化实验[J].食品与发酵工业,2006,32(5):131-133.
[110]Mercier A,Gauthier S F,Fliss I.Immunomodulating effects of whey proteins and their enzymatic digests[J].International Dairy Journal,2004,14:175-183.
[111]赵芊,王洪允,江冀,胡蓓.质谱与蛋白质和多肽的定量分析[J].药物分析,2005,25(2):244-247.
[112]Wilbert S M,Engfissei G,Yau E K,Grainger D J,Tatalick L,Axworthy D B.Quantitative analysis of a synthetic peptides,NR58-3.14.3,in serum by LC-MS with inclusion of a diastereomer as internal standard[J].Analytical Biochemistry,2000,278:14-21.
[113]梁振,张维冰,张玉奎.CZE-ESI-MS联用测定小肽混合物的研究[J].高等学校化学学报,2004,25(3):448-450.
[114]Opiteck G J,Lewis K C,Jorgenson J W,Anderegg R J.Comprehensive on-line LC/LC/MS of proteins[J].Analytical Chemistry,1997,69(8):1518-1524.
[115]中华人民共和国行业标准QB 1805.3-93.
[116]韩雅珊主编.食品化学实验指导[M].北京:北京农业大学出版社,1992.47.
[117]西北农学院汇编.基础生物化学实验指导[M].杨凌:西北农学院,1984.43-45.
[118]张水华主编.食品分析[M].北京:中国轻工业出版社,2004.156-160.
[119]廖俊仪编著.食品分析实验讲义[M].广州:暨南大学出版社,2002.13-14.
[120]郭兴凤.蛋白质水解度的测定[J].中国油脂,2000,25(6):176-177.
[121]赵新淮,冯志彪.蛋白质水解物水解度的测定[J].食品科学,1994,179(11):65-67.
[122]邓后勤,夏延斌,邓友好,危小湘.鱼制品脱腥技术研究进展[J].食品与发酵工业,2006,32(5):109-112.
[123]林莉.玉米蛋白酶解制备高F值低聚肽及应用研究[D].哈尔滨:东北农业大学硕士学位论文,2003.26.
[124]Jens Adler-Nissen.Enzymic Hydrolysis of Food Proteins[M].London:Elsevier,1985.132-152.
[125]裘迪红,周涛,戴志远,娄永江.鲐鱼蛋白水解液脱苦脱腥的研究[J].食品科学,2001,22(5):37-39.
[126]李林.辛烯基琥珀酸桃胶酯的研究[D].广州:暨南大学硕士学位论文,2007.20.
[127]张永秀,王世平,周若兰.Flavourzyme蛋白酶酶解牛骨制备低聚肽的处理条件研究[J].食品工业科技,2006,27(7):141-143.
[128]罗艳玲,欧仕益.大孔树脂在食品活性成分分离中的应用[J].食品与机械,2005,21(5):81-83.
[129]周林,蔡妙颜,郭祁远,覃宇悦.大孔吸附树脂应用的研究进展[J].昆明理工大学学报,2003,28(6):99.
[130]汪洪武,刘艳清.大孔吸附树脂的应用研究进展[J].中药材,2005,28(4):353-356.
[131]张虹,柳正良,王洪泉.大孔吸附树脂在药学领域的应用[J].中国医药工业杂志,2001,32(1):41-44.
[132]宓晓黎,杨焱,成恒嵩.大孔吸附树脂技术应用于复方中成药制备工艺的研究[J].离子交换与吸附,1997,13(6):594-598.
[133]赵彦杰.用树脂法提取龙葵果紫红色素的研究[J].西北农林科技大学学报(自然科学版),2006,34(9):93-97.
[134]高生彬,赵华,刘洪杰,刘润静,胡永琪.大孔吸附树脂对维生素C脱色研究[J].河北化工,2006,29(12):17-18.
[135]中华人民共和国国家推荐标准GB/T 5009.124-2003.
[136]李红,李炳奇,刘红,毛雁升.几种大孔树脂对甘草黄酮吸附及解吸性能的研究[J].现代食品科技,2007,23(1):11-12,16.
[137]陆健.蛋白质纯化技术及应用[M].北京:化学工业出版社,2005.37-38.
[138]刘子立,欧仕益,黄雪松,罗艳玲,谢静,徐海英,蒋笃孝.离子交换法从酶解液中提取阿魏酸[J].中药材,2004,27(12):910-913.
[139]赵彦杰.树脂法提取毛樱桃红色素的研究[J].食品科学,2007,28(9):219-222.
[140]张华,杨鑫,张春英,王静.玉米蛋白中可溶性蛋白水解氨基酸组成的测定[J].中国粮油学报,2007,22(6):19-22.
[141]邵金良,黎其万,刘家富,刘宏程,董宝生,汪禄祥,和丽忠,束继红.青刺尖中水解氨基酸含量测定分析[J].中国食品添加剂,2007,(5):148-152.
[142]刘雅楠,王青华,赵征.蓝纹干酪成熟期间氨基酸的变化研究[J].中国乳品工业,2008,36(1):22-25.
[2]孔美兰,吉宏武,章超桦.罗非鱼下脚料自溶条件的初步探讨[J].湛江海洋大学学报,2005,25(4):27-31.
[3]吴燕燕,李来好.罗非鱼片干的微波烘烤技术[J].渔业现代化,2002,(3):31-33.
[4]吴文龙.罗非鱼鱼丸的加工工艺研究[J].食品科技,2006,(2):39-41.
[5]邓后勤,夏延斌,曹小彦,危小湘,曹薇,徐发.用罗非鱼碎肉制作鱼松的生产工艺研究[[J].现代食品科技,2005,21(3):80-82.
[6]洪鹏志,杨萍,章超桦,吉宏武,刘书成,郝记明,张静.酶解罗非鱼下脚料制备的超微罗非鱼骨粉营养价值分析[J].食品与机械,2007,23(3):125-126,131.
[7]段振华,汪菊兰,王志国,易美华.罗非鱼的加工技术[J].科学养鱼,2006,1:69-70.
[8]段振华,尚军,徐松,王锡彬,易美华.罗非鱼的热风干燥特性及其主要成分含量变化研究[J].食品科学,2006,27(12):479-482.
[9]刁石强,李来好,杨贤庆,吴燕燕,徐泽智.冻罗非鱼片加工技术工艺研究[J].制冷,2005,24(3):6-10.
[10]粟桂娇,阎欲晓,申柯,覃子海.酶法制取罗非鱼水解动物蛋白的工艺研究[J].食品科学,2005,26(4):177-182.
[11]朱志伟,曾庆孝,林奕封,张立彦,阮征.采用控制酶解法从罗非鱼肉中制备血管紧张素转化酶抑制肽[J].食品与发酵工业,2004,30(12):71-76.
[12]吴燕燕,李来好,岑剑伟,周婉君,杨贤庆,陈胜军,刁石强.酶法由罗非鱼加工废弃物制取调味料的研究[J].南方水产,2006,2(1):49-53.
[13]吴林泽,李从发.罗非鱼下脚料酶解产物中抗菌肽的初步研究[J].科技信息(科学教研),2007,(17):283,428.
[14]丁卓平,刘振华,李仁冬,朱卫钦.罗非鱼鱼皮胶原蛋白提取条件的研究[J].水产科技情报,2006,33(4):190-192.
[15]陈胜军,李来好,曾名勇,杨贤庆,吴燕燕,刁石强。罗非鱼鱼皮胶原蛋白降血压酶解液的制备与活性研究[J].食品科学,2005,26(8):229-233.
[16]李小勇,李洪军.高F值低聚肽研究进展[J].粮食与油脂,2006,(6):9-11.
[17]易维学,胡顺涛,娄宇.大豆低聚肽的开发与应用[J].精细与专用化学品,2001,(1):15-17.
[18]Yamakawa M.Peptide digestion and absorption in humans:portal vein hepatic vein,and peripheral venous amino acid concentration[J].Asia Pacific Journal of Clinical Nutrition,1997,(6):88-91.
[19]曹文红,章超桦,秦小明.食品蛋白来源生物活性肽[J].粮食与油脂,2007,(2):3-6.
[20]Jolles P,Parker F,Migliore D.Immunostimulating substances from human casein[J].Journal of Immumnopharmacology,1981,(3):363-369.
[21]王梅,沈辉.食物蛋白酶解物中的生物活性肽[J].氨基酸和生物资源,1997,19(1):40-43.
[22]王秋韫,庞广昌,陈庆森.免疫活性肽的研究进展与展望[J].食品科学,2002,23(7):136-139.
[23]钟英英.天然免疫调节肽的研究概况[J].重庆科技学院学报(自然科学版),2005,7(2):69-72.
[24]阚建全主编.食品化学[M].北京:中国农业大学出版社,2002.154-156.
[25]杨金良,李文平,王克诚等.促吞噬肽抗肿瘤作用的研究[J].中国医药杂志,2000,35(1):23-25.
[26]黄艳春.酶解淡水鱼蛋白制取血管紧张素转化酶抑制肽的研究[D].武汉:华中农业大学硕士学位论文,2004.4,14,16,28-29.
[27]Ferreira S H.A bradykinin-potentiating factor(BPF) present in the venom of Bathrops jararaca[J].British Journal of Pharmacology,1965,24:163-165.
[28]Li G H,Le G W,Shi Y H,Shrestha S.Angiotensin Ⅰ-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects[J].Nutrtion Research,2004,27:469-486.
[29]安桂香,庄桂东,徐振凯,姚明经,迟玉森.食物中血管紧张素转化酶抑制肽的研究进展[J].食品研究与开发,2006,27(6):173-175.
[30]Kohmura M,Nio N,Ariyosh Y.Inhibition of angiotensin-converting enzyme by synthetic peptide fragments of human-casein[J].Agricultural and Biological Chemistry,1990,54:835-836.
[31]Fujita H,Yokayama K,Yoshikawa M.Classification and antihypertensive activity of angiotensin Ⅰ-converting enzyme inhibitory peptides derived from food proteins[J].Journal of Food Science,2000,65:564-569.
[32]卢燕婉,李作孝,荣本兵.神经肽和血管性痴呆[J].医学综述,2006,12(6):348-350.
[33]刘伟.血管加压素的研究进展[J].国外医学神经病学神经外科学分册,2002,29(1):38-41.
[34]Bicego-Nahas K C,Steiner A A,Carnio E C.Antipyretic effect of arginine vasotocin in toads[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2000,278(6):1408-1414.
[35]雷治海.家畜中枢神经系统神经肽的分布定位研究进展[J].畜牧与兽医,2002,24(4):39-42.
[36]Tirelli A,De Noni I,Resmini P.Bioactive peptides in milk products[J].Italy Journal of Food Science,1997,(2):91-98.
[37]Jolles P,Loucheux-Lefebvre M H,Henschen A.Structural relatedness of κ-casein and fibrinogen γ-chain[J].Journal of Molecular Evolution,1978,(11):271-277.
[38]Fiat A M,Migliore-Samour D,Holies P.Biologically active peptides from milk proteins with emphasis on two samples concerning antitrombotic and immunomodulating activities[J].Journal of Dairy Science,1993,76:301-310.
[39]赵利,苏伟,胡火根,戴银银,杨玉玲.胶原蛋白生物活性肽的研究进展[J].食品科学,2005,26(9):578-582.
[40]Nonaka I,Katsuda S,Ohmori T.In vitro and in vivo anti-platelet effects of enzymatic hydrolysates of collagen and collagen-related peptides[J].Bioscience Biotechnology and Biochemistry,1997,61(5):712-725.
[41]Saiga A,Tanabe S,Nishimura T.Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment[J].Journal of Agricultural and Food Chemistry,2003,51:3661-3667.
[42]Hattori M,Yamaji-Tukamoto K,Kumagai H.Antioxidative activity of soluble elastin peptides[J].Journal of Agricultural and Food Chemistry,1998,46:2167-2170.
[43]Kim S K,Kim Y T,Byun H G.Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin[J].Journal of Agricultural and Food Chemistry,2001,49:1984-1989.
[44]Chen H M,Muramoto K,Yamauchi F.Antioxidative properties of histidine-containing peptides desigened from peptide fragments found in the digests of a soybean protein[J].Journal of Agriculture and Food Chemistry,1998,46:49-53.
[45]Suetsuna K,Ukeda H,Ochi H.Isolation and characterization of free radical scavenging activities peptides derived from casein[J].Journal of Nutritional Biochemistry,2000,11:128-131.
[46]彭德翔,丁卓平.抗衰老活性肽的研究进展[J].现代食品科技,2006,22(2):267-270.
[47]McFarland G A,Holliday R.Retardation of the senescence of cultured human diploid fibroblasts by camosine[J].Experimental Cell Research,1994,212:167-175.
[48]McFarland G A,Holliday R.Further evidence for the rejuvenating effects of the dipeptide L-carnosine on cultured human diploid fibroblasts[J].Experimental Gernotology,1999,34:35-45.
[49]Fischer J E.The utilization of BCAA in the treatment of hepatic coma[A].Capocaccia L,Fischer J E,Rossi-Fanelli Feds.Hepatic encephalopathy in chronic liver failure[C].New York:Plenum,1984.11-12.
[50]Chan D I,Prenner E J,Vogel H J.Tryptophan- and arginine-rich antimicrobial peptides:Structures and mechanisms of action[J].Biochimica et Biophysica Acta,2006,1758:1184-1202.
[51]Brantl V,Teschemacher H.A material with opioid activity in bovine milk and milk products[J].Naunyn-Schmeidebergs Arch Pharmacology,1979,306:301-304.
[52]Zioudrou C,Streaty R A,Klee W A.Opioid peptides derived from food proteins[J].Journal of Biology Chemistry,1979,254:2446-2449.
[53]Meisel H.Overview on milk protein-derived peptides[J].International Dairy Journal,1998,8(6):363-373.
[54]聂剑初,吴国利,张翼伸,杨绍钟,刘鸿铭合编.生物化学简明教程[M].北京:高等教育出版社,1999.15-16.
[55]舒鼎铭,覃健萍,曹永长.缩胆囊素(CCK)的生物学功能研究进展[J].饲料工业,2004,25(11):12-16.
[56]陈大庆,倪宏.缩胆囊素生物学作用的多样性[J].国外医学儿科学分册,2002,29(3):164-166
[57]Crawley J N,Corwin R L.Biological actions of cholecystokinin[J].Peptides,1994,15(4): 731-755.
[58]Nomoto S,Miyake M,Ohta M.Impaired learning and memory in OLETF rats without cholecystokinin(CCK)-A receptor[J].Physiology and Behavior,1999,66(5):869-872.
[59]Rezayat M,Ravandeh N,Zarrindast M R.Cholecystokinin and morphine-induced hypothermia[J].European Neuropsychopharmacology,1999,9(3):219-225.
[60]姜杰,熊忠,胡健饶.神经肽与学习记忆[J].生物学通报,2002,37(3):12-14.
[61]董华强,刘本国,吴琼,曹劲松,宁正祥.几种新海洋动物活性肽研究概述[J].食品科学,2005,26(9):570-573.
[62]McWilliams D F,Watson S A,Crosbee D M.Coexpression of gastrin and gastrin receptors (CCK-B and delta CCK-B) in gastrointestinal tumour cell lines[J].Gut,1998,42:795-798.
[63]Smith J P,Shin A,Wu Y.Gastrin regulates growth of human pancreatic cancer in a tonic and autocrine fashion[J].American Journal of Physiology,1996,270:1078-1084.
[64]Caplin M E,Khan K,Grimes S.Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines[J].Digestive Diseases and Sciences,2001,46:1356-1366.
[65]方富贵,章孝荣.P物质研究进展[J].动物医学进展,2005,26(1):6-8.
[66]包新民,舒斯云,包蓉,张魁华.P物质和受体在纹状体边缘区内的分布及其与学习记忆功能的关系[J].第一军医大学学报,2002,22(2):102-105.
[67]邓云川,李世荣,丛林.P物质及其受体对成纤维细胞的作用[J].实用美容整形外科杂志,2002,13(6):317-319.
[68]岑怡红,方俊,李宗军,孟德连,邓近平.制备活性肽的主要方法研究[J].现代农业科技,2006,(12):151,153.
[69]Ondetti M A,Rubin B,Cushman D W.Design of specific inhibitors of angiotensin-converting enzyme:New class of orally active antihypertensive agents[J].Science,1977,196:441-444.
[70]黎观红.食物蛋白源血管紧张素转化酶抑制肽的研究[D].无锡:江南大学博士学位论文,2005.1,5,10.
[71]王利亚,徐建荣,吴复初,姚毅敏,彭清洋.新一代甜味剂天苯二肽的合成方法及应用前景[J].江苏化工,1999,27(2):9-12.
[72]梁汉萦,刘成梅,刘伟.食源性ACE抑制肽的研究进展[J].食品研究与开发,2007,28(8):156-158.
[73]Korhonen H,Pihlanto A.Bioactive peptides:Production and functionality[J].International Dairy Journal,2006,16:945-960.
[74]Ravallec-Ple R,Wormhoudt A.Secretagogue activities in cod(Gadus morhua) and Shrimp (Penaeus aztecus) extracts and alcalase hydrolysates determined in AR4-2J pancreatic tumour cells[J].Comparative Biochemistry and Physiology Part B,2003,134:669-679.
[75]Kim Y E,Yoon S,Yu D Y,Lonnerdal B.Chung B H.Novel angiotensin-Ⅰ-converting enzyme inhibitory peptides derived from recombinant human α_(sl)-casein expressed in Escherichia coli[J].Journal of Dairy Research,1999,66:431-439.
[76]范长胜.氨基酸二肽甜味剂的开发研究进展[J].工业微生物,2002,32(2):37-40,47.
[77]汪江波.发酵工业中的膜分离技术[J].中国酿造,1999,(6):1-3.
[78]Mulder M.Basic Principles of Membrane Technology[M].Netherlands:Kluwer Academic publish.1996:1.
[79]霍建新.膜分离法的应用[J].安徽农学通报,2007,13(14):41-42.
[80]Righetti P G,Nembri F,Bossi A,Mortarino M.Continuous enzymatic hydrolysis of beta-casein and isoelectric collection of some of the biologically active peptides in an electric field[J].Biotechnology Progress,1997,13:258-264.
[81]Turgeon S L,Gauthier S F.Whey peptide fractions obtained with a two-step ultrafiltration process:Production and characterization[J].Journal of Food Science,1990,55:106-110.
[82]Pihlanto-Leppala A,Koskinen P,Paakkari I,Tupasela T,Korhonen H.Opioid whey protein peptides obtained by membrane filtration[J].IDF Bulletin,1996,311:36-38.
[83]Pihlanto-Leppala A,Koskinen P,Piilola K,Tupasela T,Korhonen H.Angiotensin Ⅰ-converting enzyme inhibitory properties of whey protein digests:Concentration and characterization of active peptides[J].Journal of Dairy Research,2000,67:53-64.
[84]尚庆坤,祝红,阎吉昌,向前.高效制备液相色谱法分离制备虻虫中的多肽样品[J].分析化学,1999,27(8):924-926.
[85]白泉,葛小娟,耿信笃.反相液相色谱对多肽的分离、纯化与制备[J].分析化学,2002,30(9):1126-1129.
[86]朱晓囡,苏志国.反相液相色谱在蛋白质及多肽分离分析中的应用[J].分析化学,2004,32(2):248-254.
[87]张玉奎.现代生物样品分离分析方法[M].北京:科学出版社,2003.18-19.
[88]Fulton S P,Afeyan N B,Gordon N F.Very high speed separations of proteins with a 20μm reverse-phase sorbent[J].Journal of Chromatography,1991,452-547.
[89]钟芳,张晓梅,麻建国.大豆肽的离子交换色谱分离及其活性评价[J].食品与机械,2006,22(5):16-19.
[90]张晓峰,陈庆森,庞广昌.液相色谱在多肽物质分离分析上的应用[J].食品科学,2006,27(3):239-241.
[91]Application Note No.Separation of Tryptophan and Methionine Oxidized Peptides from Their Unoxidized Forms,129 Dionex Corporation,Sunnyvale,CA,2002,5.
[92]钟芳,张晓梅,麻建国.大豆肽的大孔吸附树脂以及凝胶过滤色谱分离[J].食品与机械,2006,22(4):25-28,45.
[93]田益玲,陈冠华,祝彦忠.高效毛细管电泳在食品添加剂中应用的研究进展[J].河北农业大学学报,2002,25:227-229.
[94]李卫红,徐宁,杨宝刚,丘竹贤.电泳研究及应用的进展[J].有色矿冶,1998,(3):18-21.
[95]Jorgenson J W,Lukacs K D.Zone electrophoresis in open-tubular class capillaries[J].Analytical Chemistry,1981,53(8):1298-1302.
[96]舒友琴,罗国安.毛细管电泳在食品分析中的应用[J].食品科学,1999,7:8-12.
[97]刘静,陈均志.大豆多肽分离纯化方法研究进展[J].食品工业科技,2006,27(9):171-174.
[98]刘展眉,熊绪杰,吕钱江,万岩雄.毛细管电泳及其在天然产物分离分析中的应用进展[J].仲凯农业技术学院学报,2002,15(3):59-65.
[99]冯淑波,陈萍.高效毛细管电泳的应用[J].保定师范专科学校学报,2006,19(2):31-33.
[100]孟祥平,刘建学.毛细管电泳在食品分析中的应用进展[J].食品与发酵工业,2005,31(11):88-92.
[101]Issaq H J,Janini G M,Atamana I Z,Muschik G M,Lukszo J.Capillary electrophoresis separation of small peptides,effect of pH buffer additives,and temperature[J].Journal of Liquid Chromatography,1992,15:1129-1142.
[102]Kristensen H K,Hansen S H.Separation of polymyxins by mieellar electrokinetie capillary chromatography[J].Journal of Chromatography,1993,628:309-315.
[103]Knox J H,Grant I H.Electrochromatography in packed tubes using 1.5 to 50μm silica gels and ODS bonded silica gels[J].Chromatographia,1991,32(7-8):317-328.
[104]王喜波,迟玉杰.活性炭色谱法分离芳香族氨基酸制备高F值蛋清低聚肽混合物的研究[J].食品工业科技,2005,26(6):55-57.
[105]Nokihara K.Procedures leading to primary structure determination of proteins in complex mixtures by gel electrophoresis and modern micro-scale analysis[J].Analytica Chimica Acta,1998,372(1-2):21-32.
[106]梁振江,纪明慧,李繁,黄澜.粘度法测定高聚物相对分子质量方法的改进[J].海南师范学院学报(自然科学版).2002,15(1):73-77.
[107]马永强,赵毅,石彦国.大豆蛋白水解物中肽分子分布的研究[J].中国粮油学报,2001,16(3):15-17.
[108]陈均志,张海平,顾玲,陈立红.高效毛细管电泳—紫外检测法分离测定微波复合酶法大豆蛋白水解物中的多肽[J].食品工业科技,2005,26(8):163-165.
[109]汤国营,戴军,蔡木易,易维学,谷瑞增.大豆低聚肽分子质量分布的测定及体外消化实验[J].食品与发酵工业,2006,32(5):131-133.
[110]Mercier A,Gauthier S F,Fliss I.Immunomodulating effects of whey proteins and their enzymatic digests[J].International Dairy Journal,2004,14:175-183.
[111]赵芊,王洪允,江冀,胡蓓.质谱与蛋白质和多肽的定量分析[J].药物分析,2005,25(2):244-247.
[112]Wilbert S M,Engfissei G,Yau E K,Grainger D J,Tatalick L,Axworthy D B.Quantitative analysis of a synthetic peptides,NR58-3.14.3,in serum by LC-MS with inclusion of a diastereomer as internal standard[J].Analytical Biochemistry,2000,278:14-21.
[113]梁振,张维冰,张玉奎.CZE-ESI-MS联用测定小肽混合物的研究[J].高等学校化学学报,2004,25(3):448-450.
[114]Opiteck G J,Lewis K C,Jorgenson J W,Anderegg R J.Comprehensive on-line LC/LC/MS of proteins[J].Analytical Chemistry,1997,69(8):1518-1524.
[115]中华人民共和国行业标准QB 1805.3-93.
[116]韩雅珊主编.食品化学实验指导[M].北京:北京农业大学出版社,1992.47.
[117]西北农学院汇编.基础生物化学实验指导[M].杨凌:西北农学院,1984.43-45.
[118]张水华主编.食品分析[M].北京:中国轻工业出版社,2004.156-160.
[119]廖俊仪编著.食品分析实验讲义[M].广州:暨南大学出版社,2002.13-14.
[120]郭兴凤.蛋白质水解度的测定[J].中国油脂,2000,25(6):176-177.
[121]赵新淮,冯志彪.蛋白质水解物水解度的测定[J].食品科学,1994,179(11):65-67.
[122]邓后勤,夏延斌,邓友好,危小湘.鱼制品脱腥技术研究进展[J].食品与发酵工业,2006,32(5):109-112.
[123]林莉.玉米蛋白酶解制备高F值低聚肽及应用研究[D].哈尔滨:东北农业大学硕士学位论文,2003.26.
[124]Jens Adler-Nissen.Enzymic Hydrolysis of Food Proteins[M].London:Elsevier,1985.132-152.
[125]裘迪红,周涛,戴志远,娄永江.鲐鱼蛋白水解液脱苦脱腥的研究[J].食品科学,2001,22(5):37-39.
[126]李林.辛烯基琥珀酸桃胶酯的研究[D].广州:暨南大学硕士学位论文,2007.20.
[127]张永秀,王世平,周若兰.Flavourzyme蛋白酶酶解牛骨制备低聚肽的处理条件研究[J].食品工业科技,2006,27(7):141-143.
[128]罗艳玲,欧仕益.大孔树脂在食品活性成分分离中的应用[J].食品与机械,2005,21(5):81-83.
[129]周林,蔡妙颜,郭祁远,覃宇悦.大孔吸附树脂应用的研究进展[J].昆明理工大学学报,2003,28(6):99.
[130]汪洪武,刘艳清.大孔吸附树脂的应用研究进展[J].中药材,2005,28(4):353-356.
[131]张虹,柳正良,王洪泉.大孔吸附树脂在药学领域的应用[J].中国医药工业杂志,2001,32(1):41-44.
[132]宓晓黎,杨焱,成恒嵩.大孔吸附树脂技术应用于复方中成药制备工艺的研究[J].离子交换与吸附,1997,13(6):594-598.
[133]赵彦杰.用树脂法提取龙葵果紫红色素的研究[J].西北农林科技大学学报(自然科学版),2006,34(9):93-97.
[134]高生彬,赵华,刘洪杰,刘润静,胡永琪.大孔吸附树脂对维生素C脱色研究[J].河北化工,2006,29(12):17-18.
[135]中华人民共和国国家推荐标准GB/T 5009.124-2003.
[136]李红,李炳奇,刘红,毛雁升.几种大孔树脂对甘草黄酮吸附及解吸性能的研究[J].现代食品科技,2007,23(1):11-12,16.
[137]陆健.蛋白质纯化技术及应用[M].北京:化学工业出版社,2005.37-38.
[138]刘子立,欧仕益,黄雪松,罗艳玲,谢静,徐海英,蒋笃孝.离子交换法从酶解液中提取阿魏酸[J].中药材,2004,27(12):910-913.
[139]赵彦杰.树脂法提取毛樱桃红色素的研究[J].食品科学,2007,28(9):219-222.
[140]张华,杨鑫,张春英,王静.玉米蛋白中可溶性蛋白水解氨基酸组成的测定[J].中国粮油学报,2007,22(6):19-22.
[141]邵金良,黎其万,刘家富,刘宏程,董宝生,汪禄祥,和丽忠,束继红.青刺尖中水解氨基酸含量测定分析[J].中国食品添加剂,2007,(5):148-152.
[142]刘雅楠,王青华,赵征.蓝纹干酪成熟期间氨基酸的变化研究[J].中国乳品工业,2008,36(1):22-25.