低氧低温环境大鼠睡眠剥夺后血清TNF-α和IL-6水平与大脑病理变化相关性研究
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摘要
目的:模拟高原低氧低温环境,对大鼠进行不同时间的睡眠剥夺,研究其血清肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)浓度变化及大脑病理学改变,探讨TNF-α、IL-6在缺氧、冷应激、睡眠剥夺后的变化、表达的生物学效应及与机体之间的影响关系,以便为高原睡眠疾患的发病机制研究提供新的理论依据,更好的了解和解决高原睡眠问题,提高高原人群的睡眠质量和整体健康水平。
方法:选用成年健康Sprague-Dawley大鼠,按随机原则分为5组,每组5只:正常对照组(CC)、大平台实验对照组(TC)和低氧低温环境下睡眠剥夺1 d组、睡眠剥夺4d组、睡眠剥夺7d组。建立低氧低温条件下的睡眠剥夺模型,对SD大鼠进行不同时段的睡眠剥夺后分别取血和大脑,采用酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)检测血清TNF-α和IL-6的浓度,脑组织切片进行HE染色,光镜观察病理变化。数据结果用SPSS15.0统计软件进行分析。
结果:ELISA法检测显示大鼠在低氧低温环境中睡眠剥夺后,血清TNF-α浓度随着睡眠剥夺时间的延长而呈升高趋势,血清IL-6浓度随睡眠剥夺时间延长呈现为先升高而后有所下降,但均显著高于正常对照组和大平台实验对照组。大脑HE染色显示随着缺氧冷刺激及睡眠剥夺时间的延长,脑组织水肿逐渐加重,神经胶质细胞增生明显,血管充血明显,少数神经细胞变性坏死。
结论:①大鼠在低氧低温环境中睡眠剥夺后,随着时间的延长血中细胞因子TNF-α和IL-6的表达发生了改变,TNF-α呈持续升高的趋势,IL-6呈先升高后略有下降的趋势,但较之CC组和TC组均显著升高。②在缺氧冷应激和睡眠剥夺的影响下,大鼠大脑病理切片显示出水肿、神经胶质细胞增生、血管充血,甚至出现少数神经细胞变性坏死。③大鼠血清TNF-α和IL-6的表达水平及大脑组织病理学表现与缺氧、冷应激、睡眠剥夺的严重程度及持续时间有关。④缺氧、冷应激和睡眠剥夺引起的血清TNF-α和IL-6的增高,短期可能对机体脑细胞具有应激性保护作用,而长期持续增高可能对神经元有损伤作用。
Objective:It researches on the concentration of TNF-αand IL-6 in blood serum level and pathological changes in brain of sleep deprivation in Sprague-Dawley rats under hypoxia and hypothermia conditions,and probes into the relationship between the TNF-αand IL-6 and sleep deprivation under hypoxia and hypothermia conditions and the effect on the organism,in order to provide a new theoretical basis about pathogenesis of plateau sleep disorders,comprehend and resovel the issue of high altitude sleep,improve the sleep quality and overall level of health with highland populations.
Methods:Healthy adult Sprague-Dawleys were selected at random in five groups. There are 5 Sprague-Dawley rats in each group.The five groups include antitheses group(CC group for normal and TC group for environment) and sleep deprivation group(SD1d,SD4d,SD7d) under hypoxia and hypothermia conditions.The research establishes sleep deprivation model.Having deprived of sleep at different time,the blood and brain were taken out.ELISA(Enzyme-linked immunosorbent assay) were used to test the concentration of the blood serum of TNF-αand IL-6.The brain tissue was sliced and stained in HE.Then the author observes the pathological changes with microscope.All the results are analyzed by SPSS13.0.
Results:ELISA test indicates that under hypoxia and hypothermia conditions the concentration of blood serum TNF-αtends to rise as the sleep deprivation prolongs while the concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs.But the concentrations of blood serum TNF-αand IL-6 are higher than that of the CC and TC.The HE staining of the brain shows that the brain tissue edema tends to intensify,the glial cells tend to proliferate dramatically,the blood vessels tend to engorge noticeably and few neuronal degeneration and cytoclasis as the sleep deprivation prolongs under hypoxia and hypothermia conditions.
Conclusions:①The expression of TNF-αand IL-6 in blood serum and the pathomorphology of the brain tissue changed as the sleep deprivation of SD rats under hypoxia and hypothermia conditions prolonged,the concentration of blood serum TNF-αtends to rise as the sleep deprivation prolongs while the concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs.But the concentrations of blood serum TNF-αand IL-6 are higher than that of the CC and TC.②The HE staining of the brain shows that the brain tissue edema tends to intensify,the glial cells tend to proliferate dramatically,the blood vessels tend to engorge noticeably and few neuronal degeneration and cytoclasis as the sleep deprivation prolongs under hypoxia and hypothermia conditions.③The level of TNF-αand IL-6 in the blood serum of SD rats and the histopathological chang of cerebrum were related to the degree and duration of hypoxia,hypothermia and sleep deprivation.④The rise in TNF-αand IL-6 caused by hypoxia,hypothermia and sleep deprivation might possibly protect the brain cells in the short run.But long-term continued increase of their level would likely injure the neuron.
方法:选用成年健康Sprague-Dawley大鼠,按随机原则分为5组,每组5只:正常对照组(CC)、大平台实验对照组(TC)和低氧低温环境下睡眠剥夺1 d组、睡眠剥夺4d组、睡眠剥夺7d组。建立低氧低温条件下的睡眠剥夺模型,对SD大鼠进行不同时段的睡眠剥夺后分别取血和大脑,采用酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)检测血清TNF-α和IL-6的浓度,脑组织切片进行HE染色,光镜观察病理变化。数据结果用SPSS15.0统计软件进行分析。
结果:ELISA法检测显示大鼠在低氧低温环境中睡眠剥夺后,血清TNF-α浓度随着睡眠剥夺时间的延长而呈升高趋势,血清IL-6浓度随睡眠剥夺时间延长呈现为先升高而后有所下降,但均显著高于正常对照组和大平台实验对照组。大脑HE染色显示随着缺氧冷刺激及睡眠剥夺时间的延长,脑组织水肿逐渐加重,神经胶质细胞增生明显,血管充血明显,少数神经细胞变性坏死。
结论:①大鼠在低氧低温环境中睡眠剥夺后,随着时间的延长血中细胞因子TNF-α和IL-6的表达发生了改变,TNF-α呈持续升高的趋势,IL-6呈先升高后略有下降的趋势,但较之CC组和TC组均显著升高。②在缺氧冷应激和睡眠剥夺的影响下,大鼠大脑病理切片显示出水肿、神经胶质细胞增生、血管充血,甚至出现少数神经细胞变性坏死。③大鼠血清TNF-α和IL-6的表达水平及大脑组织病理学表现与缺氧、冷应激、睡眠剥夺的严重程度及持续时间有关。④缺氧、冷应激和睡眠剥夺引起的血清TNF-α和IL-6的增高,短期可能对机体脑细胞具有应激性保护作用,而长期持续增高可能对神经元有损伤作用。
Objective:It researches on the concentration of TNF-αand IL-6 in blood serum level and pathological changes in brain of sleep deprivation in Sprague-Dawley rats under hypoxia and hypothermia conditions,and probes into the relationship between the TNF-αand IL-6 and sleep deprivation under hypoxia and hypothermia conditions and the effect on the organism,in order to provide a new theoretical basis about pathogenesis of plateau sleep disorders,comprehend and resovel the issue of high altitude sleep,improve the sleep quality and overall level of health with highland populations.
Methods:Healthy adult Sprague-Dawleys were selected at random in five groups. There are 5 Sprague-Dawley rats in each group.The five groups include antitheses group(CC group for normal and TC group for environment) and sleep deprivation group(SD1d,SD4d,SD7d) under hypoxia and hypothermia conditions.The research establishes sleep deprivation model.Having deprived of sleep at different time,the blood and brain were taken out.ELISA(Enzyme-linked immunosorbent assay) were used to test the concentration of the blood serum of TNF-αand IL-6.The brain tissue was sliced and stained in HE.Then the author observes the pathological changes with microscope.All the results are analyzed by SPSS13.0.
Results:ELISA test indicates that under hypoxia and hypothermia conditions the concentration of blood serum TNF-αtends to rise as the sleep deprivation prolongs while the concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs.But the concentrations of blood serum TNF-αand IL-6 are higher than that of the CC and TC.The HE staining of the brain shows that the brain tissue edema tends to intensify,the glial cells tend to proliferate dramatically,the blood vessels tend to engorge noticeably and few neuronal degeneration and cytoclasis as the sleep deprivation prolongs under hypoxia and hypothermia conditions.
Conclusions:①The expression of TNF-αand IL-6 in blood serum and the pathomorphology of the brain tissue changed as the sleep deprivation of SD rats under hypoxia and hypothermia conditions prolonged,the concentration of blood serum TNF-αtends to rise as the sleep deprivation prolongs while the concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs.But the concentrations of blood serum TNF-αand IL-6 are higher than that of the CC and TC.②The HE staining of the brain shows that the brain tissue edema tends to intensify,the glial cells tend to proliferate dramatically,the blood vessels tend to engorge noticeably and few neuronal degeneration and cytoclasis as the sleep deprivation prolongs under hypoxia and hypothermia conditions.③The level of TNF-αand IL-6 in the blood serum of SD rats and the histopathological chang of cerebrum were related to the degree and duration of hypoxia,hypothermia and sleep deprivation.④The rise in TNF-αand IL-6 caused by hypoxia,hypothermia and sleep deprivation might possibly protect the brain cells in the short run.But long-term continued increase of their level would likely injure the neuron.
引文
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