棉针织物复合酶精练研究
摘要
角质酶是一种可以降解植物角质的水解酶,也能分解甘油三酯和脂肪酸酯等,对于以聚合物形式存在的酯类化合物角质酶的分解能力更强。作为一种多功能酶,角质酶可以应用于生物催化、洗涤剂、食品等很多领域。此外,它还在合成塑料降解方面起到重要作用。用于纺织工业是角质酶近年来的新的应用方向,特别是在棉织物酶精练加工中的应用。
本文以江南大学自制的角质酶和果胶酶为研究对象,进行棉针织物的精练研究。通过研究角质酶处理后棉针织物各项性能的变化考察角质酶的精练效果;将角质酶和果胶酶复合,比较单一果胶酶和复合酶精练的效果差异。考察角质酶对果胶酶精练的促进作用,并对复合酶精练机理做了初步的研究。
试验结果表明:单独使用角质酶可以提高棉针织物的润湿性能,热水预处理能提高角质酶的作用效果;非离子表面活性剂能够提高角质酶对棉织物的精练效果。通过单因素试验明确了角质酶浓度、处理时间、pH以及预处理条件对角质酶处理的影响规律。角质酶和果胶酶复合酶精练结果显示:角质酶对果胶去除效果较差,而复合酶果胶去除率比单独果胶酶要高;复合酶对润湿性能提升较大。
棉蜡是决定棉纤维润湿性能的关键物质,角质酶和果胶酶去除的棉蜡成分的种类与数量可能存在差异。角质酶能够分解角质等疏水性酯类物质,而果胶酶去除的是附着在果胶质上的棉蜡,这部分间接去除的棉蜡对润湿性的影响不如疏水性酯类物质对润湿性的影响大,导致果胶酶对润湿性的改善不如角质酶。
Cutinase is an enzyme of hydrolyzing plant cuticle and it can also hydrolysis other fatty acid ester and triglyceride. But the capability for hydrolyzing polyester is better for cutinase. As a multifunctional enzyme, cutinase usually used in biocatalysis, detergents, food industry and degradation of synthetic plastics.The textile industry has become a new field for the application of cutinase in recent years.
In this paper cotton knitted fabrics were scoured by cutinase, pectinase and cutinase /pectinase, respectively. The scouring effects were investigated in terms of removal percentage of pectins, removal percentage of cotton waxes, tensile strength and wetting ability of the treated fabrics. We studied the catalytic role of cutinase in the pectinase scouring. The results showed that cutinase can improve the wetting ability of cotton knitted fabric, particularly containing a hot water pretreatment before treatment, the wetting ability was markedly improved. The non-ionic surfactants can improve the scouring effects of cutinases.
The factors such as cutinase concentration, treatment time, pH and pretreatment conditions were studied through single factor circulation experiments.The scouring results using cutinase and pectinase complex showed that cutinase has very poor ability in remaoving pectins. The pectin removal percentage using complex enzymes was higher than pectinase alone.The complex enzymes significantly improved the wetting ability of cotton knitted fabrics Cutin may be the main reason affecting the wetting ability of cotton fiber.The com- ponents of cotton wax which was removed by cutinase and pectinase may be different.
Cutinase can hydrolysis cutin and other hydrophobic esters, but pectinase can just remove the cotton wax that is attached to the pectin.The effect of this part cotton wax is inferior to the wax removed by cutinase.So the wetting ability of cotton frabic treated by cutinase is better than pectinase.
本文以江南大学自制的角质酶和果胶酶为研究对象,进行棉针织物的精练研究。通过研究角质酶处理后棉针织物各项性能的变化考察角质酶的精练效果;将角质酶和果胶酶复合,比较单一果胶酶和复合酶精练的效果差异。考察角质酶对果胶酶精练的促进作用,并对复合酶精练机理做了初步的研究。
试验结果表明:单独使用角质酶可以提高棉针织物的润湿性能,热水预处理能提高角质酶的作用效果;非离子表面活性剂能够提高角质酶对棉织物的精练效果。通过单因素试验明确了角质酶浓度、处理时间、pH以及预处理条件对角质酶处理的影响规律。角质酶和果胶酶复合酶精练结果显示:角质酶对果胶去除效果较差,而复合酶果胶去除率比单独果胶酶要高;复合酶对润湿性能提升较大。
棉蜡是决定棉纤维润湿性能的关键物质,角质酶和果胶酶去除的棉蜡成分的种类与数量可能存在差异。角质酶能够分解角质等疏水性酯类物质,而果胶酶去除的是附着在果胶质上的棉蜡,这部分间接去除的棉蜡对润湿性的影响不如疏水性酯类物质对润湿性的影响大,导致果胶酶对润湿性的改善不如角质酶。
Cutinase is an enzyme of hydrolyzing plant cuticle and it can also hydrolysis other fatty acid ester and triglyceride. But the capability for hydrolyzing polyester is better for cutinase. As a multifunctional enzyme, cutinase usually used in biocatalysis, detergents, food industry and degradation of synthetic plastics.The textile industry has become a new field for the application of cutinase in recent years.
In this paper cotton knitted fabrics were scoured by cutinase, pectinase and cutinase /pectinase, respectively. The scouring effects were investigated in terms of removal percentage of pectins, removal percentage of cotton waxes, tensile strength and wetting ability of the treated fabrics. We studied the catalytic role of cutinase in the pectinase scouring. The results showed that cutinase can improve the wetting ability of cotton knitted fabric, particularly containing a hot water pretreatment before treatment, the wetting ability was markedly improved. The non-ionic surfactants can improve the scouring effects of cutinases.
The factors such as cutinase concentration, treatment time, pH and pretreatment conditions were studied through single factor circulation experiments.The scouring results using cutinase and pectinase complex showed that cutinase has very poor ability in remaoving pectins. The pectin removal percentage using complex enzymes was higher than pectinase alone.The complex enzymes significantly improved the wetting ability of cotton knitted fabrics Cutin may be the main reason affecting the wetting ability of cotton fiber.The com- ponents of cotton wax which was removed by cutinase and pectinase may be different.
Cutinase can hydrolysis cutin and other hydrophobic esters, but pectinase can just remove the cotton wax that is attached to the pectin.The effect of this part cotton wax is inferior to the wax removed by cutinase.So the wetting ability of cotton frabic treated by cutinase is better than pectinase.
引文
1. Etters J N.New developments in high performance textiles[J].Textile Chemist and Colorist & American Dyestuff Reporter,1999,1(2):30-31.
2.李群,赵昔慧.酶在纺织印染工业中的应用[M].化工工业出版社,2006,118-125.
3.范雪荣.纺织品染整工业学[M].北京:中国纺织出版社,2006,1-2.
4. Styliani K,Diomi MEffect of pectate lyase bioscouring on physical chemical [J].Bioresource Technology, 2008,81(99):8185-8192.
5.王菊生,孙恺.染整工艺原理(第二册) [M].北京:纺织工业出版社,1987,73-86.
6. Pramod B .Agrawal, Vincent A.Wax removal for accelerated cotton scouring with alkaline pectinase [J].Biotechnology Journal, 2007, 43(5): 306-315.
7.万清余,范雪荣,王强等.纯棉织物生物酶前处理工艺的研究[J].纺织学报, 2004, 25(3):91-93.
8. Protonentis L,Lamm E Jr,Showmaker H,et al.Single bath pectinase biopreparation and dyeing of cotton.Proceedings of the Annual International Conference&Exhibition of the American Association of Textile Chemists&Colorists,2000,9(17-20):449-460.
9.罗巨涛.棉织物生物前处理的理论和实践(上) [J].染整技术,2001,23(5):12-16.
10.周文龙.酶在纺织中的应用[M].中国纺织出版社,2002,206-277.
11.刘欣.食品酶学[M].北京:中国轻工业出版社,2006,5-7.
12.陈惠黎,李文杰.分子酶学[M].北京:人民卫生出版社,1983,58-79.
13.张树政.酶制剂工业(下册)[M].北京:科学出版社,1984,34-39.
14.荆丽珍,王宝维,魏笑笑,冯强,于世浩,王亚超.绿色饲料添加剂-果胶酶的研究[J].饲料工业,2007,28 (14):6-8.
15.陈坚,华兆哲.新型纺织酶制剂的发酵与应用[M].北京:化学工业出版社,2007,171-172.
16.何中琴.酶精练的评定方法和一浴酶退浆、精练[J].印染译丛,2000,(2):27-31.
17.何中琴.原果胶酶的基础和它在棉纤维精练中的应用[J].印染译丛,1997,(8):74-81.
18.何中琴.利用生态学的纤维加工以原果胶质可溶性酶的纤维精练为主[J].印染译丛,2000,(2):35-39.
19. Sakai T, Masuda A. Scouring of fiber [P].日本专利, JP0620772, 1994-08-09.
20.何中琴.用浸轧法连续酶精练的可能性[J].印染译丛,2001,(5):27-31.
21. Etters J N.Emerging opportunities for enzymes in textiles[Z].Colourage Annual,1998.87 -92.
22. Etters J N.Cotton preparation with alkaline pectinase:an environmental advance[J]. Textile Chemist and Colorist & American Dyestuff Reporter, 1999, 79(3):33-36.
23. Etters J N, Husain P A, Lange N K.Alkaline pectinase:an eco-friendly approach to cotton preparation[J].Textile Asia,1999, 79(5) :83-85.
24. Etters J N,Lange N K,Husain.P.A.Scouring the matrix[J].Americans Textiles International, 1999,28 (5):132-135.
25. Etters J N,Condon B D,Husain P A ,et al. Dyeing properties of caustic scoured versus alkaline pectinase prepared fabric[Z].Colourage Annual,1999.41-46.
26. Durden D K,Etters J N,Sarkar A K,et al. Advances in commercial biopreparation of cotton with alkaline pectinase [J].AATCC Review,2001, 48(8) :28-30.
27. Li Y,Hardin I R.Enzymatic scouring of cotton:effects on structure and properties [J].TextileChemist and Colorist,1997,29(8):71-76.
28. Li Y,Hardin I R.Treating cotton with cellulases and pectinases:effects on cuticle and fiberproperties[J].Textile Research Journal,1998,68(9):671-679.
29. Li Y,Hardin I R.Enzymatic scouring of cotton-surfactants,agitation,and selection of enzymes[J].Textile Chemist and Colorist,1998,30(9):23-29.
30. Maria Thiry C.Enzymes in the toolbox[J].AATCC Review,2001,1(8):14-19.
31.王强,范雪荣,高卫东,陈坚.复合生物酶精练棉织物的漂白与染色性能[J].纺织导报,2006, (2):79:81.
32. Lenting H B M,Zwier E,Nierstrasz V A.Identifying important parameters for a continuous bioscouring process[J].Textile Research Journal,2002,72(9):825-831.
33. Hebeish A, Hashem M, ShakerN, et al. New development for combined bioscouring and bleaching of cotton based fabrics. Carbohydrate Polymers, 2009, 78: 961-972.
34. Hartzell M M, Hsieh Y. Enzymatic scouring to improve cotton fabric wettability [J].Textile Research Journal, 1998,68(4):233-241.
35.刘龙,汪志浩,张东旭,李江华,堵国成.陈坚碱性果胶酶的生物制造及其在纺织工业清洁生产中的应用研究进展[J].生物工程学报, 2009, 25(12): 1819-1828.
36.李鸿玉,厉重先,李祖明.磁性壳聚糖微球固定化果胶酶的酶学性质研究.食品科学, 2009, 30 (19) : 210-213.
37. Csiszar E,Urbánszki K,Szakács G.Biotreatment of desized cotton fabrics by commercial cellulase and xylanase enzymes[J]Molecular Catalysis B:Enzymatic,2001,11(4): 1065-1072.
38. Kolattukudy PE. Biopolyester membranes of plants: cutin and suberin. Science,1980, 208: 990-1000.
39.江昌俊.油菜花药中酯酶和柱头中角质酶的提纯[J].安徽农业大学学报,1996,(01) .
40. Cristina, Maria, Joaquim Cutinase: from molecular level to bioprocess development. B iotechnol Bioeng, 1999, 66:17-34.
41. Kolattukudy PE. Cutinase cleaning compositions. US Patent, 4981611.1991-01-01.
42. Degani O,Gepstein S,Dosoretz C G.Potential use of cutinase in enzymatic scouring of cotton fibercuticle.Appl Biochem and Biotechnol,2002,103(1):277-290.
43. Csiszár E,Losonczi A,Koczka B,et al.Degradation of lignin-containing Materials by xylanase inbiopreparation of cotton,Biotechnol 2006,(28):749-753.
44. Agrawal P B,Nierstrasz V A,Klug-Santner B G,et al.Wax removal for accelerated cotton scouringwith alkaline pectinase.Biotechnol,2007,(2):306-315.
45.毕凤珍.纺织用角质酶的发酵法生产及其应用[D].江南大学, 2005 .
46.孙越.果胶酶活性的测定方法[J]食品科技,1997,18(3):37-38.
47.顾燕松.纺织生物助剂果胶酶酶活的测定方法[J].纺织科学研究,2002,3:29-35.
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2.李群,赵昔慧.酶在纺织印染工业中的应用[M].化工工业出版社,2006,118-125.
3.范雪荣.纺织品染整工业学[M].北京:中国纺织出版社,2006,1-2.
4. Styliani K,Diomi MEffect of pectate lyase bioscouring on physical chemical [J].Bioresource Technology, 2008,81(99):8185-8192.
5.王菊生,孙恺.染整工艺原理(第二册) [M].北京:纺织工业出版社,1987,73-86.
6. Pramod B .Agrawal, Vincent A.Wax removal for accelerated cotton scouring with alkaline pectinase [J].Biotechnology Journal, 2007, 43(5): 306-315.
7.万清余,范雪荣,王强等.纯棉织物生物酶前处理工艺的研究[J].纺织学报, 2004, 25(3):91-93.
8. Protonentis L,Lamm E Jr,Showmaker H,et al.Single bath pectinase biopreparation and dyeing of cotton.Proceedings of the Annual International Conference&Exhibition of the American Association of Textile Chemists&Colorists,2000,9(17-20):449-460.
9.罗巨涛.棉织物生物前处理的理论和实践(上) [J].染整技术,2001,23(5):12-16.
10.周文龙.酶在纺织中的应用[M].中国纺织出版社,2002,206-277.
11.刘欣.食品酶学[M].北京:中国轻工业出版社,2006,5-7.
12.陈惠黎,李文杰.分子酶学[M].北京:人民卫生出版社,1983,58-79.
13.张树政.酶制剂工业(下册)[M].北京:科学出版社,1984,34-39.
14.荆丽珍,王宝维,魏笑笑,冯强,于世浩,王亚超.绿色饲料添加剂-果胶酶的研究[J].饲料工业,2007,28 (14):6-8.
15.陈坚,华兆哲.新型纺织酶制剂的发酵与应用[M].北京:化学工业出版社,2007,171-172.
16.何中琴.酶精练的评定方法和一浴酶退浆、精练[J].印染译丛,2000,(2):27-31.
17.何中琴.原果胶酶的基础和它在棉纤维精练中的应用[J].印染译丛,1997,(8):74-81.
18.何中琴.利用生态学的纤维加工以原果胶质可溶性酶的纤维精练为主[J].印染译丛,2000,(2):35-39.
19. Sakai T, Masuda A. Scouring of fiber [P].日本专利, JP0620772, 1994-08-09.
20.何中琴.用浸轧法连续酶精练的可能性[J].印染译丛,2001,(5):27-31.
21. Etters J N.Emerging opportunities for enzymes in textiles[Z].Colourage Annual,1998.87 -92.
22. Etters J N.Cotton preparation with alkaline pectinase:an environmental advance[J]. Textile Chemist and Colorist & American Dyestuff Reporter, 1999, 79(3):33-36.
23. Etters J N, Husain P A, Lange N K.Alkaline pectinase:an eco-friendly approach to cotton preparation[J].Textile Asia,1999, 79(5) :83-85.
24. Etters J N,Lange N K,Husain.P.A.Scouring the matrix[J].Americans Textiles International, 1999,28 (5):132-135.
25. Etters J N,Condon B D,Husain P A ,et al. Dyeing properties of caustic scoured versus alkaline pectinase prepared fabric[Z].Colourage Annual,1999.41-46.
26. Durden D K,Etters J N,Sarkar A K,et al. Advances in commercial biopreparation of cotton with alkaline pectinase [J].AATCC Review,2001, 48(8) :28-30.
27. Li Y,Hardin I R.Enzymatic scouring of cotton:effects on structure and properties [J].TextileChemist and Colorist,1997,29(8):71-76.
28. Li Y,Hardin I R.Treating cotton with cellulases and pectinases:effects on cuticle and fiberproperties[J].Textile Research Journal,1998,68(9):671-679.
29. Li Y,Hardin I R.Enzymatic scouring of cotton-surfactants,agitation,and selection of enzymes[J].Textile Chemist and Colorist,1998,30(9):23-29.
30. Maria Thiry C.Enzymes in the toolbox[J].AATCC Review,2001,1(8):14-19.
31.王强,范雪荣,高卫东,陈坚.复合生物酶精练棉织物的漂白与染色性能[J].纺织导报,2006, (2):79:81.
32. Lenting H B M,Zwier E,Nierstrasz V A.Identifying important parameters for a continuous bioscouring process[J].Textile Research Journal,2002,72(9):825-831.
33. Hebeish A, Hashem M, ShakerN, et al. New development for combined bioscouring and bleaching of cotton based fabrics. Carbohydrate Polymers, 2009, 78: 961-972.
34. Hartzell M M, Hsieh Y. Enzymatic scouring to improve cotton fabric wettability [J].Textile Research Journal, 1998,68(4):233-241.
35.刘龙,汪志浩,张东旭,李江华,堵国成.陈坚碱性果胶酶的生物制造及其在纺织工业清洁生产中的应用研究进展[J].生物工程学报, 2009, 25(12): 1819-1828.
36.李鸿玉,厉重先,李祖明.磁性壳聚糖微球固定化果胶酶的酶学性质研究.食品科学, 2009, 30 (19) : 210-213.
37. Csiszar E,Urbánszki K,Szakács G.Biotreatment of desized cotton fabrics by commercial cellulase and xylanase enzymes[J]Molecular Catalysis B:Enzymatic,2001,11(4): 1065-1072.
38. Kolattukudy PE. Biopolyester membranes of plants: cutin and suberin. Science,1980, 208: 990-1000.
39.江昌俊.油菜花药中酯酶和柱头中角质酶的提纯[J].安徽农业大学学报,1996,(01) .
40. Cristina, Maria, Joaquim Cutinase: from molecular level to bioprocess development. B iotechnol Bioeng, 1999, 66:17-34.
41. Kolattukudy PE. Cutinase cleaning compositions. US Patent, 4981611.1991-01-01.
42. Degani O,Gepstein S,Dosoretz C G.Potential use of cutinase in enzymatic scouring of cotton fibercuticle.Appl Biochem and Biotechnol,2002,103(1):277-290.
43. Csiszár E,Losonczi A,Koczka B,et al.Degradation of lignin-containing Materials by xylanase inbiopreparation of cotton,Biotechnol 2006,(28):749-753.
44. Agrawal P B,Nierstrasz V A,Klug-Santner B G,et al.Wax removal for accelerated cotton scouringwith alkaline pectinase.Biotechnol,2007,(2):306-315.
45.毕凤珍.纺织用角质酶的发酵法生产及其应用[D].江南大学, 2005 .
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