绵羊产毛性能的细胞及分子生物学调控机理研究
摘要
为了寻找控制绵羊主要毛用经济性状的基因座位,利用6只优质澳大利亚美利奴种公羊与配160只中国美利奴(新疆军垦型)母羊,产生的F_1代经半同胞交配后获得有性状记录的F_2代母羊68只,然后在F_2群体中选择与祖代公羊羊毛细度近似的6只公羊进行半同胞交配,获得有性状记录的F_3代个体共70只,其中有12对为双羔。记录了这138只F2、F3个体羊毛细度、密度、自然长度、伸直长度、回潮率5项指标,并测定了F_3代所有个体的初生重及六月龄重。对各性状进行统计分析,结果表明,各性状变异程度较大,性状间有不同程度的相关,从一个侧面预示着QTL的存在;同时,对不同交配组合的F2和F3进行了显著性检验,发现在细度、自然长度、伸直长度等性状上存在显著差异,从而可以证明该家系可用于以上性状的QTL分析,为进一步研究提供了参考。
引文
1.盛志廉,陈瑶生主编.数量遗传学.北京:科学出版社
2. Geldmann H. Investigation oninberitanceofquantitative characters in animal by gene markers[J].Thero, and Appl. Genet. 1975, 46:319～330
3. Tautz, D. Hypervariability of single sequences as general source for polymorphism DNA markers[J]. Nucleic Acids Res. 1989,17:6463～6471
4.吴登俊,马丁.费尔斯特.家畜基因组遗传多态性标记——微卫星标记研究进展(上) [J].国外畜牧科技,1999,26(1):33～35
5.张细全,李加琪,杨关福.动物遗传标记[M].北京,中国农业大学出版社。1997
6.龚炎长.畜牧微卫星应用[J].中国畜牧杂志,1998,34(6):51～52
7. Wntero A K, Y.M.Parsons,D.W.Cooper, L.R.Piper.Evidence of Linkage Between High-gtycinetyrosine Keratin Gene Loci and Wool Fibre Diameter in a Merino Half-family[J].Animal Genetics. 1994,25:105～108
8. Barendse W. Characterisation and linkage mapping of ten microsatellite markers derived from a sheep x hamster cell hybrid[J]. Mamm Genome, 1997, 8 (1):21～28
9. Nei M takezaki N. Estimation of genetic distance and phylogenetic trees from DNA analysis[J]. Proc 5th Word Congr genet Appl Livest Prod . Canada, 1994, 21:405～411
10. de Gortari M J,Freking B A,Cuthbertson R P, Kappes S M,Keele J W, Stone R T, Leymaster K A,Dodds K G, Crowford A M,Beattie C W. A second-generation linkage map of the sheep genome[J]. Mammalian genome,1998,9(3):204～209
11.徐宁迎.构建染色体连锁图谱的遗传标记—微卫星[J].浙江畜牧兽医.1998,23(2):10～11
12. Barker J S F. Proceedings of the 5th World congress om Genetics Applied to Livesstok Production, Canda. 1994,21:501～508
13. Lord E A, Davis G H, Dodds K G et al. Identification of Booroola carriwrs using microsatellite markers[J].Wool Technology and Sheep Breeding, 1998,46:245～249
14. Bidanel, J.P.D. Milan, C. Chevalet, et al, mapping of quantitative trait loci in F2 crosses between Meishan and Large White pig breeds in France[A] .Proc of Intern Conf on Pig Ptoduction[C],Beijing, 1998, 51～55
15.李和平.绵羊遗传连锁图研究及应用[A].第六次全国畜牧遗传标记研讨会论文集[C].1998:54～57
16. Allan M. Crawford. Sheep Lingkage Mapping:Nineteen Lingkage Groups Derived Form the Analysis of Paternal Half-Sib Famiollies[J].Genetices:1994,137(6):573～579
17. de Gortari M J,Freking B A,Cuthbertson R P, Kappes S M,Keele J W, Stone R T, Leymaster K A,Dodds K G,Crowford A M,Beattie C W. A second-generation linkage map of the sheep genome[J]. Mammalian genome, 1998,9(3):204～209
18. Maddox J F, Davies K P, Crawford A M, Hulme D J, Vaiman D, Cribiu E P, Freking B A, Beh K J, Cockett N E, Kang N, Riffkin C D, Drinkwater R, Moore S S, Dodds K G, Lumsden J M, van Stijn T C, Phua S H, Adelson D L, Burkin H R, Broom J E, Buitkamp J, Cushwa W T, Gerard E, Galloway S M, Harrison B, Hawken R J, Hiendleder S, Henry H M, Medrano J F, Paterson K A, Schibler L, Stone R T, van Hest B. An enhanced linkage map of the sheep genome comprising more than 1000 loci[J]. Genome Res, 2001,11(7):1275～1289
19.郭源梅.标记辅助选择的研究进展[A].第七次全国畜牧遗传标记研讨会论文集[C].2000:64～68
20.晏兆利.家畜育种中MAS的遗传标记与QTL[J].西南民族学院学报.1996,22(2):206～210
21. Teyssier J, Elsen J M. Three years comparison of productivity of Booroodc carriccr and non carrier Mcrinos D arlcs cwcs[M]. In: Laurie Pipered. Proceeding of 6WCGALP. NSW Australia:University of New England, 1998, Vol. 24:117～120
22. Cockett N.E, Jackson S.P, Shay T. L, et al. Chromosomal Location of the Calolipyge gene in sheep using bovine DNA markers[J].Proc Natl Acad Sci, 1994,91:3019～3023
23.樊斌.标记辅助选择及若干制约效率因素的研究[A].第七次全国畜禽遗传标记研讨会论文集[C],2000:60～63
24.张细权,吴常信.三种遗传标记及其在分析畜禽遗传多样性上的应用[A].第六次全国畜牧遗传标记研讨论文集[C],1998,6～9
25. Buchanan F C et al. A directed search for quantitative trait loci on chromosomes 4 and 7 in pigs[J] Genomics, 1994 Jul 15,22(2):397～403
26. Forbes, S,H,J.t. hogg, F. G. Buchanan, A.M. Crawford and F. W. Allend 0RF,.Microsatellite evolutionin congeneric mammals:domestic and bighorn sheep[J].Mol, Evo1.1995:12:1106～1113
27. Andersson, L. Genetic mapping of quantitative trait loci for growth and fatness in pigs[J].Science, 1994(263) 1771～1774
28. Ashwell,M.S, Rexroad, C,E. Miller, J.R.H. Efficient resolution of parentage in dogs by amplification of microsatellites[J].Anim. Genet.,1996,27:235～242
29. Schroorten C, Bovenhuis H, et al, Whole genome scan to detect quantitative trait loci for conformation and functional traits in dairy cattle[J], J Dairy Sci, 2000,83(4):795～806
30.徐宁迎,H. Thomsen, N. Reinsch. 德国奶牛奶用性状的QTL研究[A].第十次全国动物遗传育种学术讨论会论文集[C],19999:343～346
31. Miche Georges, Case history in animal improvenment:Mapping complex traits in ruminats, Molecular dissetion of traits: 229-240, CRC, Press, boca taton NEW york
32. Liu. B. H. Statistical. Genomics: Linkage Mapping and QTL Analysis[M]. Florida: CRC Press. 1998, 17: 205～208
33. Montgomery G. W., Crawford A. M., Penty J. M., Dodds K. G., Ede A. J., Henry H. M, Pierson C. A., Lord E. A. The Ovine Booroola fecundity gene(FecB) is linked to markers from a region of human chromosomee 4q[J]. Nature genetics 4, 410～414
34.王爱国.猪基因定位及其标记辅助选择在育种的应用[J].中国畜牧杂志.1998,34(3):45～48
35. Le Roy P, Naveau J, Elsen J M. et al. Evidence for a new major geneinfluencing meat quality in Pigs[J]. Genetic Research, 1990, 55: 33～40
36.鲁绍雄,猪主要数量性状主基因研究进展[J].黑龙江畜牧兽医,2000(8):27～29
37.陈红菊,岳永生.动物主基因研究进展[J].山东畜牧兽医,2001,6
38.刘榜.猪微卫星DNA定位方法的研究[J].湖北农业科学,1998,(6):65～67
39. Rotbacbild M F. Identification of quantitative trait loci and interegling candidate gene in the pig[A]. progess and progpeel proceeding 6th world conguess on Genetic Applied to live Production[C]. 1998, 26: 403～409
40. Paszek A A et al. Proc 6th WCGALP, 1998; 26: 418～421
41. Yu. T. P. et al. 1995. J, Anim. Sci. 73: 1282～1288
42.吴晓林,施启顺等.用混合模型定位一个复杂家猪家系13号染色体QTL的研究[J].遗传学报,2002,2
43. Andersson L, Haley C S, Euegren H, et al. Genetic mapping of quantitative trait loci for growth and fatness in pigs[J]. Science, 1994, 263(25). 1771～1774
44.屈彦纯,邓昌彦等.猪六号染色体部分遗传连锁图谱的构建与QTL定位[J].华中农业大学学报,2000,5
45. Walling G A et al. Proc 6th WCGALP, 1998; 26: 410～413
46. Wada Y, T Akita, T Furnkawal, et al. Quantitative trait loci (QTL) analysis in a Meishan x Goettingen cross population[A]. Proceeding of the 6th WCGALP[C]. Armidale, 1998; 26: 320～323
47. De Koning D J et al. Proc 6th WCGALP, 1998; 26: 410～413
48. Bidanel J P et al. Proc Int Conf pig Prod. 1998; 51～55
49.亏开兴,连林生.猪数量性状位点的研究现状[J].动物科学与动物医学,2000(17),5:23～25
50. Georges, M. Mapping quantitative trait loci gor prolificacy and growth by exploiting progeny testing[J], Genetics, 1995(139): 907～920
51. Charlier C, et al. The mh gene causing double muscling in cattle maps to bovine chromosome 2[J]. Mammalian Genome. 1995, 6; 788～792
52.曹红鹤,王雅春,陈幼春.五种微卫星标记在肉牛群体中的研究[J].中国农业科学,1999,32(1):78～82
53. Napolitano F et al. Explotation of microsatellites as genetic markers of beef-performance traits in Pimontese X chianina crossbred cattle[J]. J anim. Breed. Genet. 1996, 113: 157～162
54. Georges, et al, Proc. Natl. Acad. Sci. USA, 1993, 90(3), 1058～1062
55. Ashwell M s. Detection of loci affecting milk production and health traits in an elite US Holstein population using microsatellite markers[J]. Animal Genetics, 1997, 28: 216～222
56.徐宁迎,牛迎冬.德国奶牛奶用性状的QTL研究[A].第十次全国动物遗传育种学术讨论会论文集[C],1999,343～346
57. Ron M. Mapping quantitative trait loci with DNA microsatellite in a commercial dairy cattle population[J]. Animal Genetics, 1994, 25: 259～264
58. Hurt F B Cenetics of the fowl (Vot, Ⅵ). 1936: 105～112
59.邓学梅,等.基于F-2群体的鸡重要生长性状遗传分析[J].遗传学报,2001,9:23～25
60.胡刚安.家禽数量性状基因位点的分子标记[J].中国家禽,1998,(20),5:36～38
61. Riper L R, Bindon B M, Nethery R D. The Booroola Merino. Melbume: CSIRO Division of Animal Production, 1982. 9～20
62. Montgomery G, Lord E A, Penty J M, et al. The Booroola fecundity(Fec B) gene maps to sheep chromosome 6[J]. Genomiocs, 1994, 22: 148～153
63. Montgomerg G M, Penty J M, Lord E A, et al. Mapping producion traits in farm animals The Boorooloo (FecB)Locus, In; Burnside E B ed. Proceedings of 5WCGALP. Guelph: University of Guelph, 1994, Vol. 21; 17～20
64.姜运良,杜立新.Booroola Merino绵羊多态基因Fec。的研究进展[J].中国畜牧杂志,1999,35(4):51～53
65. Noelle E. Cockett. Chromosomal Localization of the Callipyge Gene in Sheep (Ovis Arics) Using Bovinc DNA Markers. 1994
66.王慧,杜立新.滩羊多羔性能的RAPD标记研究[J].中国畜牧杂志,2001,37(2):22～23
67. Y. M Parsons, D. W. Cooper, L. R. Piper Evidence of Linkage Between High-glycine-tyrosine Keratin Gene Loci and Wool Fibre Diameter in a Merino Half-family[J]. Genetics, 1994, 25: 105～108
68.赵俊丽,吴登俊.绵羊QTL定位的研究进展[J].四川畜牧兽医,2001,28(10):29～30
69.刘守仁,李芙蓉编著.羊毛与羊毛品质[M].新疆科技卫生出版社.1995
70.王文正,王韦.人角化细胞在三种培养法中生长的观察[J].第二军医大学学报.1985,6(6)
71.田占标,周薇.含硫氨基酸促羊毛生长研究进展及对策[J].国外畜牧学,草食家畜.1992.4.
72.郁枫.影响羊毛生长的遗传和环境因素[J].国外畜牧学-草食家畜.1993:1
73.徐子伟.绵羊毛囊活性与羊毛生长[J].国外畜牧学-草食家畜.1990.4
74.吕中法,刘荣卿.细胞因子与毛囊生长周期[J].国外医学、生理、病理科学与临床分册.1999.19(6)
75.伍津津,刘荣卿.重建毛囊的组织学研究[J].第三军医大学学报.1998.2(20)
76.伍津津,刘荣卿.人各段毛囊上皮细胞培养的增殖能力比较[J].第三军医大学学报.1999.11(21)
77.屠军波,杨壮群.毛囊的体外培养及相关生长因子研究进展[J].中国美容医学.2001,4(10)
78.秦俭,钟志红.Williams E无血清培养基中培养人头皮毛囊[J].中华皮肤科杂志.1999.3(32)
79. Philpott MP, SandersDA, KealeyT. Effects of insulin and insulin like growth factors on cultured human hair follicles: IGF I atphysiologic concentrations is an important regulator of hair follicle growth in vitro [J]. J InvestDermatol. 1994, 102(6): 857～861.
80.伍喜林,杨凤.类胰岛素样因子(IGF)的营养生理作用[J].畜牧与兽医.2002.11(34)
81.傅琳玲,朱文元.药物对毛囊细胞的影响研究[J].国外医学皮肤性病学分册.1997,2(23)
82. Hung-Yi su, Jonathan G, Hickford. Insulin-like growth factor-land hair growth[J]. Dermatologyt online Journal. 2000, 5(2): 1
83. JonesJI, Clemmons. Insulin-like growth factors and their binding proteins[J]: Biolgical actions. Endocrine Rev 1995: 16: 3～34.
84. Seiberg M, Marthinuss J, Stenn KS. Changes in expression of apoptosis-associated genes in skin mark early catagen[J]. J Invest Dermatol 1995; 104: 78～82.
85. Su H-Y, Jay NP, Gourley TS. Wool production in transgenic sheep-results from first generation adults and second-generation lambs[J]. Anita Biotechnol 1998; 9: 135～147.
86. Peus D, Pittelkow MR. Growth factors in hair organ development and the hair growth[J]. J Invest Dermatol 1996; 108: 79～87.
87. Harris PM, McBride BW, Gurnsey MP. Direct infusion of a variant of insulin-like growth factor-Ⅰ into skin of sheep and effects on local blood flow, amino acid utilisation and cell replication[J]. J Endocrinol 1993; 139: 463～472.
88. Stewart CEH, Rotwein P. Growth, differentiation, and survival: Multiple physiological functions for insulin-like growth factors[J]. Physiol Rev 1996; 76: 1005～1026.
89. Powell BC, Rogers GE. The role of keratin proteins and their genes in the growth, structure and properties of hair. In: Jolles P, Zahn H, Hocker H (Eds.). Formation and Structure of Human Hair. Birkhauser Verlag Basel, Switzerland. 1997, 59～148.
90. Liu J-P, Baker J, Perkins AS,. Mice carrying null mutations of genes encoding insulin-like growth factor 1 (igf-1) and IGF receptor (IGF-1r)[J]. Cell 1993; 75: 59～72.
91. Reynolds AJ, Jahoda CAB: Cultured dermal papilla cells induce follicle formation and hair growth by transdifferentiation of an adult epidermis[J]. Development 1992; 115: 587～593.
92. Hardy MH: The secret life of the hair follicle[J]. Trends in Genetics I992; 8: 55～61.
93. Stones AJ, Granger SP, Jenkins G. Localisation of cytokines and their receptors in human hair follicles using immunogold histochemistry[J]. J Invest Dermatol 1994; 102: SID Abstract p627.
94. Spencer GSG, Schurmann A, Berry C. Comparison of the effects of recombinant ovine, bovine and porcine growth hormones on growth, efficiency and carcass characteristics in lambs[J]. Livest Prod Sci 1994; 37: 311～321.
95. Adams NR, Briegel JR, Rigby RDG. Responses of sheep to annual cycles in nutrition. 1. Role of endogenous growth hormone during undemutrition[J]. Anim Sci 1996; 62: 279～286.
96. Eicheler W, Huth A, Happle R, Hoffman R. Phorboi-myristate-acetate, but not interleukin-1b or insulin-like growth factor-1, regulates protein kinase C isoenzymes in human dermal papilla cells[J]. Acta Derm Venereol (Stockh) 1997; 77: 361～364.
97. Philpott MP, Sanders DA, Kealey T. Effects of insulin-like growth factors on cultured human hair follicles: IGF-I at physiological concentrations is an important regulator of hair follicle growth in vitro[J]. J Invest Dermatol 1994; 102: 857～861.
98. Hembree JR, Harmon CS, Nevines TD. Regulation of human dermal papilla cell production of insulin-like growth factor binding protein-3 by retinoic acid, glucocorticoids, and insulin-like growth factor-1 [J]. J Cell Physiol 1996; 167: 556～561.
99. Stenn KS, Lawrence L, Veis D. Expression of the bcl-2 protooncogene in the cycling adult mouse hair follicle[J]. J Invest Dermatol 1994; 103: 107～111.
100.范卫新,朱文元.肝细胞生长因子对小鼠触须毛囊体外培养的研究[J].中华皮肤科杂志.1999,5(32)
101.伍津津,刘荣卿,叶庆佾等.人头皮游离毛囊培养[J].中华皮肤科杂志,1996:8:19(4)
102. Lindner G, Botchkareva VA, Botchkareva NV. Analysis of apoptosis during hair follicle regression (catagen)[J]. Am J Pathal 1997; 151: 1601～1617.
103. Burgess W, Liu Q, Zhou J, Tang Q. The immune-endocrine loop during aging: role of growth hormone and insulin-like growth factor-I[J]. Neuroimmunomodulation 1999; 6: 56～68.
104. Tamatani M, Ogawa S, Tohyama M: Roles of bcl-2 and caspases in hypoxia-induced neuronal cell death: a possible neuroprotective mechanism of peptide growth factors[J]. Mol Brain Res 1998; 58: 27～39.
105. Hueber AO, Zornig M, Lyon D. Requirement for the CD95 receptor ligand pathway in c-myc-induced apoptosis[J]. Science 1997; 278: 1305～1309.
106. Douglas RG, Gluckman PD, Ball K. The effects of infusion of insulin-like growth factor (IGF)I, IGFII and insulin on glucose and protein metabolism in fasted lambs[J]. J Clin Invest 1991; 88: 614～622.
107. Oddy VH, Owens PC. Insulin-like growth factor I inhibits degradation and improves retention of protein in hindlimb muscle of lambs[J]. Amer J Physiol 1996; 34: E973～982.
108. Hocking Edwards JE, Khalaf SK, Sinclair BR. Metabolic response of sheep skin to a chronic infusion of a variant of insulin-like growth factor I[J]. Biochem J 1995; 308: 411～418.
109.赵锦荣,阎小君,苏成芝.差异显示反转录PCR技术研究进展[J].生物化学与生物物理进展.2000,27(1):28～32
110.钟金城,陈智华.mRNA差异显示技术及其在动物遗传育种中的应用[J].黄牛杂志.1999,25(1):10～12
111.Carl W. Dieffenbach, Gabriela S. Dveksler著,黄培堂等译。PCR技术实验指南[M].1999.295～310
112.池晓菲,舒庆尧.开放的差异基因表达技术研究进展[J].中国生物化学与分子生物学报.2002,18(3):259～264
113.马庆军,宋国立,党耕町.mRNA差异显示方法研究进展[J].中华外科杂志.2000,38(2):154～155
114.张辛燕,冯捷,李刚.mRNA差异显示筛选卵巢癌相关基因[J].北京医科大学学报.2000,32(5).431～425
115. C. E. Vallejos, J. J. Malandro, K. Sheehy M. J. Zimmermann. Detection and cloning of expressed sequences linked to a target gene. Theor Appl Genet. 2000, 101: 1109-1113
116. V. H. -C. Liaoa and J. H. Freedmanb, Differential display analysis of gene expression in invertebrates[J]. CMLS, Cell. Mol. Life Sci. 2002,: 1256～1263
117. A. V. Ivanova, S. V. Ivanov. Differential display analysis of gene expression in yeast[J]. CMLS, Cell. Mol. Life Sci. 2002, 59: 1241～1245
118. J. Stein, P. Liang. Differential display technology: a general guide[J]. CMLS, Cell. Mol. Life Sci. 2002, 59: 1235～1240
119. Liang P, PardeeAB. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction[J]. Science. 1992, 257(5072): 967～971
120.郝纯毅,赵敏,李勇等.降低mRNA差异显示技术假阳性率的一种方法[J].中国生物化学与分子生物学报.2002,18(1):110～114
121.王宏,李春海,陈高明等.mRNA差异显示技术中差异条带的回收与再扩增[J].中国肿瘤临床.1999,26(5):350～351
122.李子银,陈受宦.mRNA差异显示阳性cDNA克隆的快速筛选与鉴定[J].高技术通讯.1999.8:44～48
123.徐国恒,凌雁梁,德勇.银染mRNA差异显示方法的建立和吗啡诱导基因的克隆[J].北京医科大学学报.1998,30(5)
124. Liang P, ZhuW, ZhangX. Differential display using one base anchored oligo dT primers[J]. Nucleic AcidsRes. 1994, 22(25): 5763～5764
125.梁德勇,崔振中,王晓民.mRNA差异显示技术的研究进展[J].中国兽医学报.1999,19(6):618～621
126. Makoto Itoh, Shuichi Tsukada, Takuya Orita etal. Identification by differential display of eight known genes induced during in vivo intimal hyperplasia[J]. J Hum Genet. 1998, 43: 9～13
127. Yunfeng Zhu, Jia Luo, Joshua Barker etal. Identification of genes induced by rapid intraoperative tissue expansion in mouse skin[J]. Arch Dermatol Res. 2002, 293: 560～568
128. Nephew KP, Whaley AE, Christenson RK, Imakawa K. Differential expression of distinct mRNAs for ovine trophoblast protein-1 and related sheep type Ⅰ interferons[J], Biol Reprod. 1993, 48(4): 768～78.
129.潘佩文,赵叔红,刘榜.利用差显技术检测中外猪种肌肉组织差异表达基因的初步研究[A].中国动物遗传育种研究进展,第11次全国动物遗传育种学会会议论文[C].1999:92～95
130. M. D. Li, Overexpression of B-subunit of thyroid-stimulation hormore in Meishan swine identified by differential display[J]. J, Anim, Sci. 1996, 74: 2104～2111
131. F. Schmoll, K. Wimmers, Porcine ESTs detected by differential display representing possible candidated for the traitseye muscle area[J]. Journal of Animal breeding and genetics. 2000, 117: 25～35
132. Claute Robert, Differential display and suppressive subtractive hybridization used to identify granulosa cell messenger RNA associated with bovine occyte developmental competence[J]. Biology of Reproduction. 2001, 64: 910～917
133. Dean Tatlow. Differential display analysis of gene expression during the induction of mucosal immunity[J], Immunogenetics, 2000, 52(23): 73～80
134. YunFengZhu, Idenfication of estrogen-responsive genes in chick liver[J]. Cell and Tissue research. 2001, 305(9): 357～363
135. A. M. Gillett, Pre-and postnatal lung development, maturation and plasticity inceased expression of the lung stimulates calmodulion expressionin the sheep[J]. Immunogenetics, 2002, 54(3): 18～22
136. Karl Hilgers, Angiolensin dependent gene expression in the developing rat kidney[J]. Kidney International 1998, 54(67): 146～153
137. Makoto Itoh, Identification by differential display of eight known genes induced during in vivo intimal hyperplasia[J]. Journal of human genticss. 1998, 43(1): 9～13
138. Akiya, Watakabe, Journal of neurochemistry, 2001, 76(5): 1445～1452
139. Ute Dorroch, Isolation and characterization of hepatic and intestinal expressed sepuence taqs potentially invoved in trait differentiation between cows of different
2. Geldmann H. Investigation oninberitanceofquantitative characters in animal by gene markers[J].Thero, and Appl. Genet. 1975, 46:319～330
3. Tautz, D. Hypervariability of single sequences as general source for polymorphism DNA markers[J]. Nucleic Acids Res. 1989,17:6463～6471
4.吴登俊,马丁.费尔斯特.家畜基因组遗传多态性标记——微卫星标记研究进展(上) [J].国外畜牧科技,1999,26(1):33～35
5.张细全,李加琪,杨关福.动物遗传标记[M].北京,中国农业大学出版社。1997
6.龚炎长.畜牧微卫星应用[J].中国畜牧杂志,1998,34(6):51～52
7. Wntero A K, Y.M.Parsons,D.W.Cooper, L.R.Piper.Evidence of Linkage Between High-gtycinetyrosine Keratin Gene Loci and Wool Fibre Diameter in a Merino Half-family[J].Animal Genetics. 1994,25:105～108
8. Barendse W. Characterisation and linkage mapping of ten microsatellite markers derived from a sheep x hamster cell hybrid[J]. Mamm Genome, 1997, 8 (1):21～28
9. Nei M takezaki N. Estimation of genetic distance and phylogenetic trees from DNA analysis[J]. Proc 5th Word Congr genet Appl Livest Prod . Canada, 1994, 21:405～411
10. de Gortari M J,Freking B A,Cuthbertson R P, Kappes S M,Keele J W, Stone R T, Leymaster K A,Dodds K G, Crowford A M,Beattie C W. A second-generation linkage map of the sheep genome[J]. Mammalian genome,1998,9(3):204～209
11.徐宁迎.构建染色体连锁图谱的遗传标记—微卫星[J].浙江畜牧兽医.1998,23(2):10～11
12. Barker J S F. Proceedings of the 5th World congress om Genetics Applied to Livesstok Production, Canda. 1994,21:501～508
13. Lord E A, Davis G H, Dodds K G et al. Identification of Booroola carriwrs using microsatellite markers[J].Wool Technology and Sheep Breeding, 1998,46:245～249
14. Bidanel, J.P.D. Milan, C. Chevalet, et al, mapping of quantitative trait loci in F2 crosses between Meishan and Large White pig breeds in France[A] .Proc of Intern Conf on Pig Ptoduction[C],Beijing, 1998, 51～55
15.李和平.绵羊遗传连锁图研究及应用[A].第六次全国畜牧遗传标记研讨会论文集[C].1998:54～57
16. Allan M. Crawford. Sheep Lingkage Mapping:Nineteen Lingkage Groups Derived Form the Analysis of Paternal Half-Sib Famiollies[J].Genetices:1994,137(6):573～579
17. de Gortari M J,Freking B A,Cuthbertson R P, Kappes S M,Keele J W, Stone R T, Leymaster K A,Dodds K G,Crowford A M,Beattie C W. A second-generation linkage map of the sheep genome[J]. Mammalian genome, 1998,9(3):204～209
18. Maddox J F, Davies K P, Crawford A M, Hulme D J, Vaiman D, Cribiu E P, Freking B A, Beh K J, Cockett N E, Kang N, Riffkin C D, Drinkwater R, Moore S S, Dodds K G, Lumsden J M, van Stijn T C, Phua S H, Adelson D L, Burkin H R, Broom J E, Buitkamp J, Cushwa W T, Gerard E, Galloway S M, Harrison B, Hawken R J, Hiendleder S, Henry H M, Medrano J F, Paterson K A, Schibler L, Stone R T, van Hest B. An enhanced linkage map of the sheep genome comprising more than 1000 loci[J]. Genome Res, 2001,11(7):1275～1289
19.郭源梅.标记辅助选择的研究进展[A].第七次全国畜牧遗传标记研讨会论文集[C].2000:64～68
20.晏兆利.家畜育种中MAS的遗传标记与QTL[J].西南民族学院学报.1996,22(2):206～210
21. Teyssier J, Elsen J M. Three years comparison of productivity of Booroodc carriccr and non carrier Mcrinos D arlcs cwcs[M]. In: Laurie Pipered. Proceeding of 6WCGALP. NSW Australia:University of New England, 1998, Vol. 24:117～120
22. Cockett N.E, Jackson S.P, Shay T. L, et al. Chromosomal Location of the Calolipyge gene in sheep using bovine DNA markers[J].Proc Natl Acad Sci, 1994,91:3019～3023
23.樊斌.标记辅助选择及若干制约效率因素的研究[A].第七次全国畜禽遗传标记研讨会论文集[C],2000:60～63
24.张细权,吴常信.三种遗传标记及其在分析畜禽遗传多样性上的应用[A].第六次全国畜牧遗传标记研讨论文集[C],1998,6～9
25. Buchanan F C et al. A directed search for quantitative trait loci on chromosomes 4 and 7 in pigs[J] Genomics, 1994 Jul 15,22(2):397～403
26. Forbes, S,H,J.t. hogg, F. G. Buchanan, A.M. Crawford and F. W. Allend 0RF,.Microsatellite evolutionin congeneric mammals:domestic and bighorn sheep[J].Mol, Evo1.1995:12:1106～1113
27. Andersson, L. Genetic mapping of quantitative trait loci for growth and fatness in pigs[J].Science, 1994(263) 1771～1774
28. Ashwell,M.S, Rexroad, C,E. Miller, J.R.H. Efficient resolution of parentage in dogs by amplification of microsatellites[J].Anim. Genet.,1996,27:235～242
29. Schroorten C, Bovenhuis H, et al, Whole genome scan to detect quantitative trait loci for conformation and functional traits in dairy cattle[J], J Dairy Sci, 2000,83(4):795～806
30.徐宁迎,H. Thomsen, N. Reinsch. 德国奶牛奶用性状的QTL研究[A].第十次全国动物遗传育种学术讨论会论文集[C],19999:343～346
31. Miche Georges, Case history in animal improvenment:Mapping complex traits in ruminats, Molecular dissetion of traits: 229-240, CRC, Press, boca taton NEW york
32. Liu. B. H. Statistical. Genomics: Linkage Mapping and QTL Analysis[M]. Florida: CRC Press. 1998, 17: 205～208
33. Montgomery G. W., Crawford A. M., Penty J. M., Dodds K. G., Ede A. J., Henry H. M, Pierson C. A., Lord E. A. The Ovine Booroola fecundity gene(FecB) is linked to markers from a region of human chromosomee 4q[J]. Nature genetics 4, 410～414
34.王爱国.猪基因定位及其标记辅助选择在育种的应用[J].中国畜牧杂志.1998,34(3):45～48
35. Le Roy P, Naveau J, Elsen J M. et al. Evidence for a new major geneinfluencing meat quality in Pigs[J]. Genetic Research, 1990, 55: 33～40
36.鲁绍雄,猪主要数量性状主基因研究进展[J].黑龙江畜牧兽医,2000(8):27～29
37.陈红菊,岳永生.动物主基因研究进展[J].山东畜牧兽医,2001,6
38.刘榜.猪微卫星DNA定位方法的研究[J].湖北农业科学,1998,(6):65～67
39. Rotbacbild M F. Identification of quantitative trait loci and interegling candidate gene in the pig[A]. progess and progpeel proceeding 6th world conguess on Genetic Applied to live Production[C]. 1998, 26: 403～409
40. Paszek A A et al. Proc 6th WCGALP, 1998; 26: 418～421
41. Yu. T. P. et al. 1995. J, Anim. Sci. 73: 1282～1288
42.吴晓林,施启顺等.用混合模型定位一个复杂家猪家系13号染色体QTL的研究[J].遗传学报,2002,2
43. Andersson L, Haley C S, Euegren H, et al. Genetic mapping of quantitative trait loci for growth and fatness in pigs[J]. Science, 1994, 263(25). 1771～1774
44.屈彦纯,邓昌彦等.猪六号染色体部分遗传连锁图谱的构建与QTL定位[J].华中农业大学学报,2000,5
45. Walling G A et al. Proc 6th WCGALP, 1998; 26: 410～413
46. Wada Y, T Akita, T Furnkawal, et al. Quantitative trait loci (QTL) analysis in a Meishan x Goettingen cross population[A]. Proceeding of the 6th WCGALP[C]. Armidale, 1998; 26: 320～323
47. De Koning D J et al. Proc 6th WCGALP, 1998; 26: 410～413
48. Bidanel J P et al. Proc Int Conf pig Prod. 1998; 51～55
49.亏开兴,连林生.猪数量性状位点的研究现状[J].动物科学与动物医学,2000(17),5:23～25
50. Georges, M. Mapping quantitative trait loci gor prolificacy and growth by exploiting progeny testing[J], Genetics, 1995(139): 907～920
51. Charlier C, et al. The mh gene causing double muscling in cattle maps to bovine chromosome 2[J]. Mammalian Genome. 1995, 6; 788～792
52.曹红鹤,王雅春,陈幼春.五种微卫星标记在肉牛群体中的研究[J].中国农业科学,1999,32(1):78～82
53. Napolitano F et al. Explotation of microsatellites as genetic markers of beef-performance traits in Pimontese X chianina crossbred cattle[J]. J anim. Breed. Genet. 1996, 113: 157～162
54. Georges, et al, Proc. Natl. Acad. Sci. USA, 1993, 90(3), 1058～1062
55. Ashwell M s. Detection of loci affecting milk production and health traits in an elite US Holstein population using microsatellite markers[J]. Animal Genetics, 1997, 28: 216～222
56.徐宁迎,牛迎冬.德国奶牛奶用性状的QTL研究[A].第十次全国动物遗传育种学术讨论会论文集[C],1999,343～346
57. Ron M. Mapping quantitative trait loci with DNA microsatellite in a commercial dairy cattle population[J]. Animal Genetics, 1994, 25: 259～264
58. Hurt F B Cenetics of the fowl (Vot, Ⅵ). 1936: 105～112
59.邓学梅,等.基于F-2群体的鸡重要生长性状遗传分析[J].遗传学报,2001,9:23～25
60.胡刚安.家禽数量性状基因位点的分子标记[J].中国家禽,1998,(20),5:36～38
61. Riper L R, Bindon B M, Nethery R D. The Booroola Merino. Melbume: CSIRO Division of Animal Production, 1982. 9～20
62. Montgomery G, Lord E A, Penty J M, et al. The Booroola fecundity(Fec B) gene maps to sheep chromosome 6[J]. Genomiocs, 1994, 22: 148～153
63. Montgomerg G M, Penty J M, Lord E A, et al. Mapping producion traits in farm animals The Boorooloo (FecB)Locus, In; Burnside E B ed. Proceedings of 5WCGALP. Guelph: University of Guelph, 1994, Vol. 21; 17～20
64.姜运良,杜立新.Booroola Merino绵羊多态基因Fec。的研究进展[J].中国畜牧杂志,1999,35(4):51～53
65. Noelle E. Cockett. Chromosomal Localization of the Callipyge Gene in Sheep (Ovis Arics) Using Bovinc DNA Markers. 1994
66.王慧,杜立新.滩羊多羔性能的RAPD标记研究[J].中国畜牧杂志,2001,37(2):22～23
67. Y. M Parsons, D. W. Cooper, L. R. Piper Evidence of Linkage Between High-glycine-tyrosine Keratin Gene Loci and Wool Fibre Diameter in a Merino Half-family[J]. Genetics, 1994, 25: 105～108
68.赵俊丽,吴登俊.绵羊QTL定位的研究进展[J].四川畜牧兽医,2001,28(10):29～30
69.刘守仁,李芙蓉编著.羊毛与羊毛品质[M].新疆科技卫生出版社.1995
70.王文正,王韦.人角化细胞在三种培养法中生长的观察[J].第二军医大学学报.1985,6(6)
71.田占标,周薇.含硫氨基酸促羊毛生长研究进展及对策[J].国外畜牧学,草食家畜.1992.4.
72.郁枫.影响羊毛生长的遗传和环境因素[J].国外畜牧学-草食家畜.1993:1
73.徐子伟.绵羊毛囊活性与羊毛生长[J].国外畜牧学-草食家畜.1990.4
74.吕中法,刘荣卿.细胞因子与毛囊生长周期[J].国外医学、生理、病理科学与临床分册.1999.19(6)
75.伍津津,刘荣卿.重建毛囊的组织学研究[J].第三军医大学学报.1998.2(20)
76.伍津津,刘荣卿.人各段毛囊上皮细胞培养的增殖能力比较[J].第三军医大学学报.1999.11(21)
77.屠军波,杨壮群.毛囊的体外培养及相关生长因子研究进展[J].中国美容医学.2001,4(10)
78.秦俭,钟志红.Williams E无血清培养基中培养人头皮毛囊[J].中华皮肤科杂志.1999.3(32)
79. Philpott MP, SandersDA, KealeyT. Effects of insulin and insulin like growth factors on cultured human hair follicles: IGF I atphysiologic concentrations is an important regulator of hair follicle growth in vitro [J]. J InvestDermatol. 1994, 102(6): 857～861.
80.伍喜林,杨凤.类胰岛素样因子(IGF)的营养生理作用[J].畜牧与兽医.2002.11(34)
81.傅琳玲,朱文元.药物对毛囊细胞的影响研究[J].国外医学皮肤性病学分册.1997,2(23)
82. Hung-Yi su, Jonathan G, Hickford. Insulin-like growth factor-land hair growth[J]. Dermatologyt online Journal. 2000, 5(2): 1
83. JonesJI, Clemmons. Insulin-like growth factors and their binding proteins[J]: Biolgical actions. Endocrine Rev 1995: 16: 3～34.
84. Seiberg M, Marthinuss J, Stenn KS. Changes in expression of apoptosis-associated genes in skin mark early catagen[J]. J Invest Dermatol 1995; 104: 78～82.
85. Su H-Y, Jay NP, Gourley TS. Wool production in transgenic sheep-results from first generation adults and second-generation lambs[J]. Anita Biotechnol 1998; 9: 135～147.
86. Peus D, Pittelkow MR. Growth factors in hair organ development and the hair growth[J]. J Invest Dermatol 1996; 108: 79～87.
87. Harris PM, McBride BW, Gurnsey MP. Direct infusion of a variant of insulin-like growth factor-Ⅰ into skin of sheep and effects on local blood flow, amino acid utilisation and cell replication[J]. J Endocrinol 1993; 139: 463～472.
88. Stewart CEH, Rotwein P. Growth, differentiation, and survival: Multiple physiological functions for insulin-like growth factors[J]. Physiol Rev 1996; 76: 1005～1026.
89. Powell BC, Rogers GE. The role of keratin proteins and their genes in the growth, structure and properties of hair. In: Jolles P, Zahn H, Hocker H (Eds.). Formation and Structure of Human Hair. Birkhauser Verlag Basel, Switzerland. 1997, 59～148.
90. Liu J-P, Baker J, Perkins AS,. Mice carrying null mutations of genes encoding insulin-like growth factor 1 (igf-1) and IGF receptor (IGF-1r)[J]. Cell 1993; 75: 59～72.
91. Reynolds AJ, Jahoda CAB: Cultured dermal papilla cells induce follicle formation and hair growth by transdifferentiation of an adult epidermis[J]. Development 1992; 115: 587～593.
92. Hardy MH: The secret life of the hair follicle[J]. Trends in Genetics I992; 8: 55～61.
93. Stones AJ, Granger SP, Jenkins G. Localisation of cytokines and their receptors in human hair follicles using immunogold histochemistry[J]. J Invest Dermatol 1994; 102: SID Abstract p627.
94. Spencer GSG, Schurmann A, Berry C. Comparison of the effects of recombinant ovine, bovine and porcine growth hormones on growth, efficiency and carcass characteristics in lambs[J]. Livest Prod Sci 1994; 37: 311～321.
95. Adams NR, Briegel JR, Rigby RDG. Responses of sheep to annual cycles in nutrition. 1. Role of endogenous growth hormone during undemutrition[J]. Anim Sci 1996; 62: 279～286.
96. Eicheler W, Huth A, Happle R, Hoffman R. Phorboi-myristate-acetate, but not interleukin-1b or insulin-like growth factor-1, regulates protein kinase C isoenzymes in human dermal papilla cells[J]. Acta Derm Venereol (Stockh) 1997; 77: 361～364.
97. Philpott MP, Sanders DA, Kealey T. Effects of insulin-like growth factors on cultured human hair follicles: IGF-I at physiological concentrations is an important regulator of hair follicle growth in vitro[J]. J Invest Dermatol 1994; 102: 857～861.
98. Hembree JR, Harmon CS, Nevines TD. Regulation of human dermal papilla cell production of insulin-like growth factor binding protein-3 by retinoic acid, glucocorticoids, and insulin-like growth factor-1 [J]. J Cell Physiol 1996; 167: 556～561.
99. Stenn KS, Lawrence L, Veis D. Expression of the bcl-2 protooncogene in the cycling adult mouse hair follicle[J]. J Invest Dermatol 1994; 103: 107～111.
100.范卫新,朱文元.肝细胞生长因子对小鼠触须毛囊体外培养的研究[J].中华皮肤科杂志.1999,5(32)
101.伍津津,刘荣卿,叶庆佾等.人头皮游离毛囊培养[J].中华皮肤科杂志,1996:8:19(4)
102. Lindner G, Botchkareva VA, Botchkareva NV. Analysis of apoptosis during hair follicle regression (catagen)[J]. Am J Pathal 1997; 151: 1601～1617.
103. Burgess W, Liu Q, Zhou J, Tang Q. The immune-endocrine loop during aging: role of growth hormone and insulin-like growth factor-I[J]. Neuroimmunomodulation 1999; 6: 56～68.
104. Tamatani M, Ogawa S, Tohyama M: Roles of bcl-2 and caspases in hypoxia-induced neuronal cell death: a possible neuroprotective mechanism of peptide growth factors[J]. Mol Brain Res 1998; 58: 27～39.
105. Hueber AO, Zornig M, Lyon D. Requirement for the CD95 receptor ligand pathway in c-myc-induced apoptosis[J]. Science 1997; 278: 1305～1309.
106. Douglas RG, Gluckman PD, Ball K. The effects of infusion of insulin-like growth factor (IGF)I, IGFII and insulin on glucose and protein metabolism in fasted lambs[J]. J Clin Invest 1991; 88: 614～622.
107. Oddy VH, Owens PC. Insulin-like growth factor I inhibits degradation and improves retention of protein in hindlimb muscle of lambs[J]. Amer J Physiol 1996; 34: E973～982.
108. Hocking Edwards JE, Khalaf SK, Sinclair BR. Metabolic response of sheep skin to a chronic infusion of a variant of insulin-like growth factor I[J]. Biochem J 1995; 308: 411～418.
109.赵锦荣,阎小君,苏成芝.差异显示反转录PCR技术研究进展[J].生物化学与生物物理进展.2000,27(1):28～32
110.钟金城,陈智华.mRNA差异显示技术及其在动物遗传育种中的应用[J].黄牛杂志.1999,25(1):10～12
111.Carl W. Dieffenbach, Gabriela S. Dveksler著,黄培堂等译。PCR技术实验指南[M].1999.295～310
112.池晓菲,舒庆尧.开放的差异基因表达技术研究进展[J].中国生物化学与分子生物学报.2002,18(3):259～264
113.马庆军,宋国立,党耕町.mRNA差异显示方法研究进展[J].中华外科杂志.2000,38(2):154～155
114.张辛燕,冯捷,李刚.mRNA差异显示筛选卵巢癌相关基因[J].北京医科大学学报.2000,32(5).431～425
115. C. E. Vallejos, J. J. Malandro, K. Sheehy M. J. Zimmermann. Detection and cloning of expressed sequences linked to a target gene. Theor Appl Genet. 2000, 101: 1109-1113
116. V. H. -C. Liaoa and J. H. Freedmanb, Differential display analysis of gene expression in invertebrates[J]. CMLS, Cell. Mol. Life Sci. 2002,: 1256～1263
117. A. V. Ivanova, S. V. Ivanov. Differential display analysis of gene expression in yeast[J]. CMLS, Cell. Mol. Life Sci. 2002, 59: 1241～1245
118. J. Stein, P. Liang. Differential display technology: a general guide[J]. CMLS, Cell. Mol. Life Sci. 2002, 59: 1235～1240
119. Liang P, PardeeAB. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction[J]. Science. 1992, 257(5072): 967～971
120.郝纯毅,赵敏,李勇等.降低mRNA差异显示技术假阳性率的一种方法[J].中国生物化学与分子生物学报.2002,18(1):110～114
121.王宏,李春海,陈高明等.mRNA差异显示技术中差异条带的回收与再扩增[J].中国肿瘤临床.1999,26(5):350～351
122.李子银,陈受宦.mRNA差异显示阳性cDNA克隆的快速筛选与鉴定[J].高技术通讯.1999.8:44～48
123.徐国恒,凌雁梁,德勇.银染mRNA差异显示方法的建立和吗啡诱导基因的克隆[J].北京医科大学学报.1998,30(5)
124. Liang P, ZhuW, ZhangX. Differential display using one base anchored oligo dT primers[J]. Nucleic AcidsRes. 1994, 22(25): 5763～5764
125.梁德勇,崔振中,王晓民.mRNA差异显示技术的研究进展[J].中国兽医学报.1999,19(6):618～621
126. Makoto Itoh, Shuichi Tsukada, Takuya Orita etal. Identification by differential display of eight known genes induced during in vivo intimal hyperplasia[J]. J Hum Genet. 1998, 43: 9～13
127. Yunfeng Zhu, Jia Luo, Joshua Barker etal. Identification of genes induced by rapid intraoperative tissue expansion in mouse skin[J]. Arch Dermatol Res. 2002, 293: 560～568
128. Nephew KP, Whaley AE, Christenson RK, Imakawa K. Differential expression of distinct mRNAs for ovine trophoblast protein-1 and related sheep type Ⅰ interferons[J], Biol Reprod. 1993, 48(4): 768～78.
129.潘佩文,赵叔红,刘榜.利用差显技术检测中外猪种肌肉组织差异表达基因的初步研究[A].中国动物遗传育种研究进展,第11次全国动物遗传育种学会会议论文[C].1999:92～95
130. M. D. Li, Overexpression of B-subunit of thyroid-stimulation hormore in Meishan swine identified by differential display[J]. J, Anim, Sci. 1996, 74: 2104～2111
131. F. Schmoll, K. Wimmers, Porcine ESTs detected by differential display representing possible candidated for the traitseye muscle area[J]. Journal of Animal breeding and genetics. 2000, 117: 25～35
132. Claute Robert, Differential display and suppressive subtractive hybridization used to identify granulosa cell messenger RNA associated with bovine occyte developmental competence[J]. Biology of Reproduction. 2001, 64: 910～917
133. Dean Tatlow. Differential display analysis of gene expression during the induction of mucosal immunity[J], Immunogenetics, 2000, 52(23): 73～80
134. YunFengZhu, Idenfication of estrogen-responsive genes in chick liver[J]. Cell and Tissue research. 2001, 305(9): 357～363
135. A. M. Gillett, Pre-and postnatal lung development, maturation and plasticity inceased expression of the lung stimulates calmodulion expressionin the sheep[J]. Immunogenetics, 2002, 54(3): 18～22
136. Karl Hilgers, Angiolensin dependent gene expression in the developing rat kidney[J]. Kidney International 1998, 54(67): 146～153
137. Makoto Itoh, Identification by differential display of eight known genes induced during in vivo intimal hyperplasia[J]. Journal of human genticss. 1998, 43(1): 9～13
138. Akiya, Watakabe, Journal of neurochemistry, 2001, 76(5): 1445～1452
139. Ute Dorroch, Isolation and characterization of hepatic and intestinal expressed sepuence taqs potentially invoved in trait differentiation between cows of different