三十烷醇（TA）纳米制剂的制备及对小麦、绿豆种苗生长的影响
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摘要
三十烷醇(TA)作为一种新型植物调节剂,目前在我国有着广泛的应用和研究。本论文在综述三十烷醇研究进展及应用的基础上,采用纳米技术方法处理,制备出三十烷醇纳米制剂,通过比较,初步研究了该制剂对小麦、绿豆种苗生长的影响。主要研究结果如下:
1、通过超声波振荡法处理制备三十烷醇的纳米制剂,经过扫描电镜及透射电镜检测及粒径计算分布软件系统分析,三十烷醇颗粒粒径可达到50-200nm;将三十烷醇纳米制剂配制成不同浓度(0-2mg/L范围内:0.5mg/L、1.0mg/L、1.5mg/L、2.0mg/L),以相同浓度TA原剂及水为对照,研究对小麦和绿豆种苗生长发育的影响。
2、不同浓度的TA纳米制剂及相同浓度的TA原剂处理后均能提高种子的萌发率和萌发势,而TA纳米制剂促进作用更加明显,其中最为明显的是0.5mg/L的处理组,TA纳米制剂处理种子后,与相同浓度TA原剂和水对照比较,萌发率分别提高了1.2%和3.2%。
3、TA纳米制剂对处理的幼苗生长促壮效应更明显,表现在增加苗高、根长、根数以及增加叶片鲜重、干重。其中最为明显的是0.5mg/L的处理组,TA纳米制剂处理后,与相同浓度TA原剂和水对照比较,小麦苗高分别增加了6.5%和8.6%、根长分别增加了7.2%和9.5%、鲜重分别增加了20.5%和21.3%、干重分别增加了23.1%和26.5%。
4、TA纳米制剂对处理的幼苗生理指标有一定影响,表现在提高叶片叶绿素含量、提高MDA含量、增加酶的活性。其中最为明显的是0.5mg/L的处理组,TA纳米制剂处理后,与相同浓度TA原剂和水对照比较,小麦叶绿素含量分别增加了26.1%和31.2%、MDA含量分别增加了11.2%和13.5%、CAT含量分别增加了20.5%和22.7%、POD含量分别增加了21.3%和26.3%。
经过对超声波振荡法、球磨法等多种纳米制剂制备方法的探索后,本研究采用超声波震荡法,此法制备的三十烷醇纳米制剂物相纯净、无杂质存在,且颗粒大小分布均匀。关于纳米生物制剂更加适宜的制备方法及其作用机理,有待进一步研究。
Triacontanol plants as a new regulator, is currently in our country have a wide range of applications and research. Summary of the papers in Triacontanol (TA) and application of research based on the means of dealing with the use of nano-technology, nano-prepared Triacontanol agents, through comparison, the preliminary study of the preparation of the wheat, the growth of mung bean seedlings impact. The main findings are as follows:
1, By ultrasonic oscillation method to deal with the preparation of nano-Triacontanol agents, after scanning electron microscopy and transmission electron microscopy detection and calculation of the distribution of particle size analysis software the particle size Triacontanol up to 50-200nm; will Triacontanol the original agents Triacontanol preparation of nano-agents at different concentrations (0-2mg / L range:0.5mg/L、1.0mg/L、1.5mg/L、2.0mg/L) to cultivate wheat and mung bean seeds and seedlings to study the different concentrations of nano-agents and general agents of wheat and mung bean seedling growth and development.
2, Different concentrations of TA can be improved germination rate and germination potential after soaking the seed, and preparations to deal with nano-TA on seed germination after the more obvious role. In, 0.5mg / L nano-agent deal with the TA after seed germination rate than the control group increased 1.2% and 3.2%..
3, TA Nano-agents to deal with the strong effect of seedling growth and promote more obvious in the increase in seedling height, root length, root number and increased leaf fresh weight, dry weight. Morphological indicators of the overall performance of the most obvious is that when the concentration of 0.5mg / L TA treated nano-formulations, compared with the control group increased by 6.5% and 8.6% in seedling height, root length increased by 7.2% and 9.5%, fresh weight increase of20.5% and 21.3%, dry weight an increase of 23.1% and 26.5%.
4, TA Nano-agents to deal with the physical growth of the seedlings have a certain influence, reflected in the chlorophyll content to increase and improve MDA content, increased the enzyme activity. Physiological indicators of the performance of the most obvious is that when the concentration of 0.5mg / L TA treated nano-formulations, compared with the control group, chlorophyll content increased by 26.1% and 31.2%, MDA content increased by 11.2% and 13.5%, CAT concentration increased by 20.5% and 22.7%, POD was increased of 21.3% and 26.3%.
Through the ultrasonic oscillation, ball milling and other methods of preparation of nano-formulations, after exploration, the selection method using ultrasonic vibration, the Triacontanol prepared phase pure nano-agents without the existence of impurities, and particle size distribution.On to explore a wide range of preparation methods of nano-biological agents and their mechanism of action, pending further study.
1、通过超声波振荡法处理制备三十烷醇的纳米制剂,经过扫描电镜及透射电镜检测及粒径计算分布软件系统分析,三十烷醇颗粒粒径可达到50-200nm;将三十烷醇纳米制剂配制成不同浓度(0-2mg/L范围内:0.5mg/L、1.0mg/L、1.5mg/L、2.0mg/L),以相同浓度TA原剂及水为对照,研究对小麦和绿豆种苗生长发育的影响。
2、不同浓度的TA纳米制剂及相同浓度的TA原剂处理后均能提高种子的萌发率和萌发势,而TA纳米制剂促进作用更加明显,其中最为明显的是0.5mg/L的处理组,TA纳米制剂处理种子后,与相同浓度TA原剂和水对照比较,萌发率分别提高了1.2%和3.2%。
3、TA纳米制剂对处理的幼苗生长促壮效应更明显,表现在增加苗高、根长、根数以及增加叶片鲜重、干重。其中最为明显的是0.5mg/L的处理组,TA纳米制剂处理后,与相同浓度TA原剂和水对照比较,小麦苗高分别增加了6.5%和8.6%、根长分别增加了7.2%和9.5%、鲜重分别增加了20.5%和21.3%、干重分别增加了23.1%和26.5%。
4、TA纳米制剂对处理的幼苗生理指标有一定影响,表现在提高叶片叶绿素含量、提高MDA含量、增加酶的活性。其中最为明显的是0.5mg/L的处理组,TA纳米制剂处理后,与相同浓度TA原剂和水对照比较,小麦叶绿素含量分别增加了26.1%和31.2%、MDA含量分别增加了11.2%和13.5%、CAT含量分别增加了20.5%和22.7%、POD含量分别增加了21.3%和26.3%。
经过对超声波振荡法、球磨法等多种纳米制剂制备方法的探索后,本研究采用超声波震荡法,此法制备的三十烷醇纳米制剂物相纯净、无杂质存在,且颗粒大小分布均匀。关于纳米生物制剂更加适宜的制备方法及其作用机理,有待进一步研究。
Triacontanol plants as a new regulator, is currently in our country have a wide range of applications and research. Summary of the papers in Triacontanol (TA) and application of research based on the means of dealing with the use of nano-technology, nano-prepared Triacontanol agents, through comparison, the preliminary study of the preparation of the wheat, the growth of mung bean seedlings impact. The main findings are as follows:
1, By ultrasonic oscillation method to deal with the preparation of nano-Triacontanol agents, after scanning electron microscopy and transmission electron microscopy detection and calculation of the distribution of particle size analysis software the particle size Triacontanol up to 50-200nm; will Triacontanol the original agents Triacontanol preparation of nano-agents at different concentrations (0-2mg / L range:0.5mg/L、1.0mg/L、1.5mg/L、2.0mg/L) to cultivate wheat and mung bean seeds and seedlings to study the different concentrations of nano-agents and general agents of wheat and mung bean seedling growth and development.
2, Different concentrations of TA can be improved germination rate and germination potential after soaking the seed, and preparations to deal with nano-TA on seed germination after the more obvious role. In, 0.5mg / L nano-agent deal with the TA after seed germination rate than the control group increased 1.2% and 3.2%..
3, TA Nano-agents to deal with the strong effect of seedling growth and promote more obvious in the increase in seedling height, root length, root number and increased leaf fresh weight, dry weight. Morphological indicators of the overall performance of the most obvious is that when the concentration of 0.5mg / L TA treated nano-formulations, compared with the control group increased by 6.5% and 8.6% in seedling height, root length increased by 7.2% and 9.5%, fresh weight increase of20.5% and 21.3%, dry weight an increase of 23.1% and 26.5%.
4, TA Nano-agents to deal with the physical growth of the seedlings have a certain influence, reflected in the chlorophyll content to increase and improve MDA content, increased the enzyme activity. Physiological indicators of the performance of the most obvious is that when the concentration of 0.5mg / L TA treated nano-formulations, compared with the control group, chlorophyll content increased by 26.1% and 31.2%, MDA content increased by 11.2% and 13.5%, CAT concentration increased by 20.5% and 22.7%, POD was increased of 21.3% and 26.3%.
Through the ultrasonic oscillation, ball milling and other methods of preparation of nano-formulations, after exploration, the selection method using ultrasonic vibration, the Triacontanol prepared phase pure nano-agents without the existence of impurities, and particle size distribution.On to explore a wide range of preparation methods of nano-biological agents and their mechanism of action, pending further study.
引文
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