糖蜜酒精废液对甘蔗和蔗田土壤影响的研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
在新植蔗植期,宿根蔗开垄松蔸时直接浇灌不同浓度糖蜜酒精废液,与常规化肥施用量和不施肥的处理作比较试验,研究直接浇灌糖蜜酒精废液对新植蔗和第一年宿根蔗的影响;同时了解一年和连续两年直接浇灌不同浓度糖蜜酒精废液对蔗田土壤一些特性的影响。结果表明:
     1.施用糖蜜酒精废液提高了新植蔗和第一年宿根蔗前中期叶片中苹果酸酶、淀粉酶、过氧化物酶、硝酸还原酶的活性及叶绿素a、叶绿素b、水溶性蛋白、全氮、全钾含量。
     2.工艺成熟期,新植蔗直接浇灌糖蜜酒精废液处理甘蔗叶片中过氧化物酶、中性转化酶、蔗糖磷酸合成酶、蔗糖合成酶(合成方向)活性低于其它处理的,同时甘蔗叶片水溶性蛋白含量增加;宿根蔗,工艺成熟期前期这些酶活性略低于其它处理的,而在后期与其它处理的相当,甘蔗品质的变化趋势与这些酶活性变化基本一致。
     3.浇灌糖蜜酒精废液的新植蔗和宿根蔗的出苗率、分蘖率、株高、有效茎数和产量显著高于施用化肥和不施肥处理的。
     4.新植蔗浇灌(45T糖蜜酒精废液+60T清水)/hm~2以及宿根蔗浇灌(75T糖蜜酒精废液+30T清水)/hm~2糖蜜酒精废液的出苗数、分蘖数、株高和产量与浇灌105T/hm~2糖蜜酒精废液的大致相同甚至略高于浇灌105T/hm~2糖蜜酒精废液的。
     5.施用糖蜜酒精废液提高了蔗地的有机质含量、全N量和速效K含量,但降低了蔗地速效P含量和蔗叶中全P含量。
Different rates of vinasse were applied when the plant cane was planted and the ratoon cane after the earth was broken from the stubbles of sugarcane. The routine fertilization and no fertilization were designed as the controls in the experiment. To investigate the effects of application of different rates of vinasse from cane sugar mill on the plant and the first ratoon crops, and to investigate the effects of vinasse on some characteristics of soil in sugarcane field after applying different rates of vinasse at sugarcane field in one or two years respectively. The results were as follows.
     1. The vinasse increased the activity of NR, NADP-malic enzyme, peroxidase, and amylase, and the contents of Chl.a, Chl.b, water soluble protein, nitrogen and potassium in leaves of the plant and the first ratoon crops of sugarcane until technical maturing stage.
     2. For the plant crop, the activity of peroxidase, neutral invertase, SPS and SS in sugarcane leaves were lower, but the content of water soluble protein in leaves were higher in the vinasse treatments than in the other treatments at the technical maturing stage. For the ratoon crops, the activities of the tested enzymes were lower in the vinasse treatments than the other treatments at early technical maturing stage, but they were similar to the other treatments later. The change tendency of cane quality was consistent with the activities of the enzymes.
     3. The emergence rate, tillering rate, plant height, millable stalks per hectare and cane yield of the plant and the ratoon crops were higher in the vinasse treatments than in the no fertilization control and the routine fertilization treatment.
     4. The emergence rate, tillering rate, millable stalks per hectare and cane yield in the plant crop in the treatments with 45t vinasse + 60t water per hectare and that with 75t vinasse + 30t water per hectare were equal to those in the treatment with 105t vinasse per hectare, or even higher.
     5. The vinasse treatments increased the the contents of organic matter, total N and available K of the soil in the field, but slightly reduced the content of available P in the soil and the content of phosphorus in the plant leaves.
引文
[1] 金其荣,金丰秋.糖蜜酒精废液综合利用与治理.酿酒,2002,29(4):63-67
    [2] 唐其展,田忠孝,朱树标,邓亚德.利用糖厂酒精废液配制的液体肥在甘蔗上的肥效研究.广西农业科学,2004,35(3):205-208
    [3] 朱国洪,刘振华,尹国,彭超英,高孔荣.甘蔗糖蜜酒精工业废液治理.四川环境,2000,19(2):45-49
    [4] Saha NK, Balakrishnan M, Batra VS. Improving industrial water use: case study for an Indian distillery. Resources, Conservation and Recycling, 2005, 43(22): 163-174
    [5] Garciaet IG, Venceslada JLB, Pena PRJ, Gomez ER. Biodegradation of phenol compounds in vinasse using Aspergillus Terreus and Geotrichum Candidum. Wat Res, 1997, 31(8): 2005-2011
    [6] 吴忠铨,梁建生,谭礼宁.糖蜜酒精废液处理探讨.广西蔗糖,2000,19(3):44-47
    [7] Wei YA, Xu YJ. Environmental management of sugar industry effluents in Gaungxi, China. Proc. Internl. Syrup. on Sustainable sugarcane & sugar Production Technol, Nanning, P. R. China, 2004: 693-697
    [8] 陶云刚.糖厂酒精废液治理措施及效果.甘蔗糖业,2000,(5):32-37
    [9] 夏学康,江祖嘉,李轩,黄祥宁.利用酒精废液生产有机—无机复合肥.广西轻工业,2003,19(3):23-26
    [10] 欧胜彬,杨辉,梁海秋,周河治,方张明.我国糖蜜酒精废液治理技术的回顾和展望.广西轻工业,2002,(4):10-12
    [11] 许喜林,林福兰,黎锡流,赖凤英.糖蜜酒精生产中的几个问题的探讨.甘蔗糖业,1998,(1):47-49
    [12] 李奇伟,戚荣,张远平.能源甘蔗生产已醇的发展前景.甘蔗糖业,2004,(5):29-33
    [13] Navarro AR, Rubio MC. Bilconcentration of venasse from the alcoholic fermentation of sugar cane molasses. Waste Management, 2000, 20: 581-585.
    [14] 陈立平,陈开正,吴鸿祥,陈央.甘蔗糖厂酒精废液堆肥实现零排放的工 程实践(续).广西轻工业,2000,(2):31-32
    [15] 陈孟林,吴颍瑞,倪小明,何星存.蜜糖酒精废液治理技术的现状与发展方向.现代化工,2002,22卷曾刊:170-174
    [16] 黄伟添,韦乐军.甘蔗糖蜜酒精废液综合利用的探索与实践.甘蔗糖业,1997,(5):41-45
    [17] 江永.利用酒精废液生产商品高效甘蔗专用有机复混肥的若干问题.甘蔗糖业,1999,(4):14-17
    [18] 徐文,李蘅,张生炎,扈胜禄,黄伟.糖蜜酒精废液生化法的研究进展.矿产与地质,2002,(16):56-60
    [19] Baskar M, Sheik dawood M, Balamurugan J, Anthoni raj S, Kandaswamy KG, Ravindran K. Potential use of molasses based on distillery industrials wastewater in agriculture through environmentally safe technologies. Proc. Internl. Symp. on Sustainable sugarcane & sugar Production Technol, Nanning, P. R. Cina, 2004: 718-724
    [20] 彭超英,朱国洪,尹国,高孔荣.甘蔗糖蜜酒精工业废液治理的研究.环境保护科学,2000,26(98):24-26
    [21] 傅其军,苏毅摘译.法国酒精发酵工业的废液处理.广西轻工业,2003,(3):21-26
    [22] Wilkie CA, Riedesel KJ, Owens JM.. Stillage characterization and anaerobic treatment of ethanol stillage from conventional and cellulose feedstocks. Biomass and Biochemistry, 2000, 19: 63-102
    [23] Cuhna RC, Costa ACS, Maser B, Cassarini DCP. Effects of irrigation with vinasse and the dynamics of its constituents in the soil: Ⅰ physical and chemical aspects. Water Science Technology, 1987, 19: 155-165
    [24] 保国裕.1998年甘蔗综合利用及废液治理技术动态.广西蔗糖,1999,(1):37-44
    [25] 胡开林,杨聪,刘惠芳.甘蔗糖厂酒精废液治理技术综述.给水排水,2003,29(1):50-58
    [26] 金江华.糖厂废醪液大面积施肥的应用及探讨.云南环境科学,1999,18(2):51-54
    [27] 李强,庞朝安,韦鸣泽.甘蔗食用糖厂酒精废液试验初报.广西蔗糖,2004(4).23-24
    [28] Casarini DCP, Cuhna RC, Maset B. Effects of irrigation with vinasse and the dynamics of its constituents in the soil: Ⅱ Microbial asPects. Water Science Technology, 1987, 19: 167-176
    [29] Gemtos TA, Chouliaras N, Marakis S. Vinasse Rate, Time of Application and Compaction Effect on Soil Properties and Durum Wheat Crop. Journal of Agricultural Engineering Research, 1999, 73: 283-296
    [30] Patil JD, Savant NK, Deshmane AN. Rate of decomposition of spent wash in soil. Journal Indian Soil Science, 1984, 32: 32-34
    [31] 金江华.糖厂废液蔗地施肥的效应.甘蔗糖业,2000(2):51-52
    [32] Gil-Sotres F, Trasar-Cepeda C, Leiros MC,. Seoane S. Different approaches to evaluating soil quality using biochemical properties. Soil Biology & Biochemistry, 2005, 37(5): 877-887
    [33] Marx MC, Wood M, Jarvis SC. A microplate fluorimetric assay for the study of enzyme diversity in soils. Soil Biology & Biochemistry, 2001, 33(8): 1633-1640
    [34] Kandeler E, Stemmer M, Klimanek EM. Response of soil microbial biomass, urease and xylanase within particle size fractions to long-term soil management. Soil Biology & Biochemistry, 1999, 31(13): 261-273
    [35] 张海燕,张旭东,李军,王冬梅.土壤微生物量测定方法概述.微生物学杂志,2005,25(4):95-99
    [36] 高云超,朱文珊,陈文新.土壤微生物生物量周转的估算[J].生态学杂志,1993,12(6):6-10
    [37] 严旭升主编.土壤肥力研究方法[M].农业出版社,1998
    [38] 周礼恺.土壤酶学[M].北京:科学出版社,1987
    [39] 邱莉萍,刘军,王益权,孙慧敏,文祥.土壤酶活性与土壤肥力的关系研究.植物营养与肥料学报,2004,10(3):277-280
    [40] Marinari S, Masciandaro G, Ceccanti B, Grego S. Influence of organic and mineral fertilisers on soil biological and physical properties. Bioresource Technology, 72: 9-17
    [41] 徐华龙.混合液法测定茶树叶片叶绿素含量[J].龙岩师专学报(自然科学版),1995,13(3):94-95
    [42] 华东师大生物系植物生理教研室编.植物生理实验指导.上海科技出版社,1980:73-75
    [43] 周树,郑相穆.硝酸还原酶体内分析方法探讨.植物生理学通讯,1995,(1):47-49
    [44] 广西大学植物生理教研组编.植物生理实验法,33-35,5-8
    [45] 林炎坤,李杨瑞,叶燕萍.三种生长调节剂对甘蔗若干生理生化特性的影响.广西 农业大学学报,1992,(3):125-130
    [46] 李杨瑞.甘蔗组织中过氧化物酶活性及其与工艺成熟的关系初探[J].广西农学院学报,1990,9(1):13-18
    [47] 范隆葆.甘蔗的几个生理生化参数与蔗糖积累的关系,甘蔗糖业,1989,(3):20-27
    [48] 李合生,陈翠莲,洪玉枝主编.植物生理生化实验原理和技术.1998:140-143
    [49] 广西农学院植物生理教研室编:甘蔗生理学实验指导,1987
    [50] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem, 1976, 72: 248-254
    [51] 上海植物生理学会主编.植物生理实验手册.上海:上海科学技术出版社,1985
    [52] 中国科学院上海植物生理研究所,上海植物生理学会主编.植物生理实验指南.上 海:上海科学技术出版社,1999
    [53] Zhu YJ, Komor E, Moore PH. Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate syntheses. Plant Phyiol, 1997, 115: 609-616
    [54] 赵越,魏自民,马凤鸣.铵态氮对甜菜蔗糖合成酶和蔗糖磷酸合成酶的影响.中国糖料,2003,(3):1-4
    [55] 鲍世旦主编.土壤农化分析,第3版.北京:中国农业出版社,2000,266:270-271
    [56] 蔡武城,袁厚积.生物物质常用化学分析法.科学出版社,1982,115-117
    [57] 张笃思主编.甘蔗制糖工业分析.北京:中国轻工业出版社,1991,34-42,46-51
    [58] 张笃思主编.甘蔗制糖工业分析.北京:中国轻工业出版社,1991,3442,46-51
    [59] 陆国盈.甘蔗检糖技术(甘蔗专业用)广西大学农学院内部讲义,1994,27-76
    [60] 王威豪,莫云川,王一丁,叶燕萍,李杨瑞.灌施糖蜜酒精废液对甘蔗苗期生长及光合特性的影响.亚热带农业研究,2006,2(2):26-30
    [61] 汪沛洪主编.植物生物化学.北京:中国农业出版社,1993,45-53
    [62] 农友业,李杨瑞,张逢友,陈锦朝.甘蔗生长后期喷施乙烯利的效应.广西农业大学学报,1998,17(3):222-226
    [63] 莫家让,周承圣编.甘蔗栽培育种生理基础.福州:福建科学技术出版社,1984
    [64] 黄卓烈,潘涛.几个甘蔗品种的过氧化物酶活性及其同工酶多样性的比较研究.华南农业大学学报,1998,19(4):72-76
    [65] 周人刚,韩雅珊,阎隆飞.植物株高与过氧化物酶的关系.作物学报,1983,9(4):267-273
    [66] Su LY, Cruz A, Moore PH and Markets A. The relationship of glyphsate treatment to sugar metabolism in sugarcane: new physic ological insights. J. Plant Physiology, 1992, 140: 168-173
    [67] Alexander AG. Hydrolytic protein of sugarcane: Amylase. J. Agric Univ.P. R, 1965, 49: 308-324
    [68] 苏广达,叶振邦,吴伯烇,李玉潜,陈俊昌编著.甘蔗栽培生物学.轻工业出版社,1980
    [69] 叶振邦.甘蔗不同品种叶片中酶活性差异研究.作物学报,1987,6(2):157-162
    [70] Huber SC. Biochemical mechanism for regulation of sucrose accumulation in leaves during photosynthesis. Plant physiology, 1989, 91: 656-662
    [71] Sehtiya HL, Dendsay JPS, Dhawan AK. Internodal invertases and stalk maturity in surgarcan. Journal of Agricultural science, Cambridge, 1991, 116(2): 239-243
    [72] 陈伟栋,陈西凯.甘蔗叶片转化酶在不同叶片的分布及叶片分类.四川甘蔗,1990(2):4-9
    [73] 陈伟栋,陈西凯.甘蔗叶片转化酶与进化生长和糖分积累的关系.四川甘蔗科技,1985,(3):27-36
    [74] Huber SC. Role of sucrose - phosphate syntheses in partitioning of carbon in leaves. Plant Physiology, 1983, 71: 818-821
    [75] 王宁宁,朱新建,王淑芳,朱亮基.苦参碱对小麦旗叶中蔗糖磷酸合成酶活性的调节.南开大学学报(自然科学报),2001,33(1):19-22
    [76] Botha FC, Black KG. sucrose phosphate syntheses and sucrose syntheses activity during maturation of internodal tissue in sugarcane. Aust. J. Plant Physiol, 2000, 27(1): 81-85
    [77] 王一丁,韦鸣泽,毕黎明,李杨瑞,王威豪,叶燕萍.定量施用糖蜜酒精废液对甘蔗苗期3种酶活性和农艺性状的影响.西南农业学报,2006,19(3):482-485

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700