马尾松毛虫质型多角体病毒全基因组序列的测定与分析
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摘要
本论文由两章组成。
第一章对质型多角体病毒的研究作了综述性介绍，包括对质型多角体病毒的分类、多角体及病毒粒子、复制及组装、基因组结构和功能等方面作了阐述。另外，本章还对马尾松毛虫质型多角体病毒的研究作了重点的介绍。
第二章进行了马尾松毛虫质型多角体病毒（DpCPV）全基因组序列的测定与分析研究。马尾松毛虫质型多角体病毒属于呼肠孤病毒科、质型多角体病毒属，基因组由十条分段的dsRNA组成。在本文中，我们完成了DpCPV全基因组序列的测定工作。对测得的序列分析表明，每一基因组片段含一个开放性阅读框，两末端存在保守的核苷酸序列，分别为5'(AGUAA)和3'(GUUAGCC)。根据DpCPV-Hn编码的蛋白和呼肠孤病毒科其它成员编码的蛋白的比较结果推测，DpCPV-Hn S1、S3、S4、S6、S7分别编码病毒的结构蛋白VP1、VP2、VP3、VP4、VP5，S2编码蛋白为病毒的依赖于RNA的RNA聚合酶（RdRp），S5、S8、S9编码蛋白为病毒的非结构蛋白，S10为多角体蛋白基因。功能性结构域分析显示，VP3 氨基酸序列部分区域与Methanosarcina mazei Goel 的甲基化酶有同源性，推测VP3在病毒mRNA的加帽过程中起重要作用。VP4含有亮氨酸锌指结构和ATP/GTP结合位点，推测VP4具有核酸结合活性。S5编码蛋白的氨基酸序列部分区域与口蹄疫病毒的2A蛋白酶（ FMDV 2Apro）非常相似。对几种CPV的RNA聚合酶基因和多角体蛋白基因的进化分析表明，DpCPV-Hn为I型CPV，和BmCPV的进化关系非常相近
There are two chapters in this thesis.
Chapter one is a general introduction of cytoplasmic polyhedrosis virus (CPV), including the classification about CPV, the polyhedrin and virus particle of CPV and gene structures and functions of CPV. The research on Dendrolimus punctatus CPV (DpCPV) also has been introduced in this chapter.
Chapter two is about the sequence determination and analyse of complete genome segments of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV). DpCPV belongs to genus Cypovirus in the family Reoviridae. Its genome consist ten dsRNA segments. Each segment posses a single open reading frame. Conserved motifs 5'(AGUAA) and 3'(GUUAGCC) were found at the ends of each segment. Comparison of proteins of DpCPV-Hn with those of other members in the family Reoviridae lead us suggest that S1, S3, S4, S6 and S7 encode viral structural protein VP1, VP2, VP3, VP4 and VP5, respectively. S5, S8 and S9 encode viral non-stuctural proteins. S2 encodes an RNA-dependent-RNA polymerase (RdRp). S10 encodes polyhedrin. Motif analysis shows that VP3 is similar to the methyltransferase of Methanosarcina mazei Goel, VP4 has motifs for leucine zipper and ATP/GTP-binding sites, and the protein encoded by DpCPV S5 is remarkably similar to foot-and-mouth disease virus 2A protease (FMDV 2Apro). Phylogenetic analysis of RdRps from nine viruses of the family Reoviridae and polyhedrin from several characterized cypoviruses indicate that DpCPV-Hn is a type 1 cypovirus, more related to Bombyx mori cypovirus (BmCPV) than to other cypovirus species
第一章对质型多角体病毒的研究作了综述性介绍，包括对质型多角体病毒的分类、多角体及病毒粒子、复制及组装、基因组结构和功能等方面作了阐述。另外，本章还对马尾松毛虫质型多角体病毒的研究作了重点的介绍。
第二章进行了马尾松毛虫质型多角体病毒（DpCPV）全基因组序列的测定与分析研究。马尾松毛虫质型多角体病毒属于呼肠孤病毒科、质型多角体病毒属，基因组由十条分段的dsRNA组成。在本文中，我们完成了DpCPV全基因组序列的测定工作。对测得的序列分析表明，每一基因组片段含一个开放性阅读框，两末端存在保守的核苷酸序列，分别为5'(AGUAA)和3'(GUUAGCC)。根据DpCPV-Hn编码的蛋白和呼肠孤病毒科其它成员编码的蛋白的比较结果推测，DpCPV-Hn S1、S3、S4、S6、S7分别编码病毒的结构蛋白VP1、VP2、VP3、VP4、VP5，S2编码蛋白为病毒的依赖于RNA的RNA聚合酶（RdRp），S5、S8、S9编码蛋白为病毒的非结构蛋白，S10为多角体蛋白基因。功能性结构域分析显示，VP3 氨基酸序列部分区域与Methanosarcina mazei Goel 的甲基化酶有同源性，推测VP3在病毒mRNA的加帽过程中起重要作用。VP4含有亮氨酸锌指结构和ATP/GTP结合位点，推测VP4具有核酸结合活性。S5编码蛋白的氨基酸序列部分区域与口蹄疫病毒的2A蛋白酶（ FMDV 2Apro）非常相似。对几种CPV的RNA聚合酶基因和多角体蛋白基因的进化分析表明，DpCPV-Hn为I型CPV，和BmCPV的进化关系非常相近
There are two chapters in this thesis.
Chapter one is a general introduction of cytoplasmic polyhedrosis virus (CPV), including the classification about CPV, the polyhedrin and virus particle of CPV and gene structures and functions of CPV. The research on Dendrolimus punctatus CPV (DpCPV) also has been introduced in this chapter.
Chapter two is about the sequence determination and analyse of complete genome segments of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV). DpCPV belongs to genus Cypovirus in the family Reoviridae. Its genome consist ten dsRNA segments. Each segment posses a single open reading frame. Conserved motifs 5'(AGUAA) and 3'(GUUAGCC) were found at the ends of each segment. Comparison of proteins of DpCPV-Hn with those of other members in the family Reoviridae lead us suggest that S1, S3, S4, S6 and S7 encode viral structural protein VP1, VP2, VP3, VP4 and VP5, respectively. S5, S8 and S9 encode viral non-stuctural proteins. S2 encodes an RNA-dependent-RNA polymerase (RdRp). S10 encodes polyhedrin. Motif analysis shows that VP3 is similar to the methyltransferase of Methanosarcina mazei Goel, VP4 has motifs for leucine zipper and ATP/GTP-binding sites, and the protein encoded by DpCPV S5 is remarkably similar to foot-and-mouth disease virus 2A protease (FMDV 2Apro). Phylogenetic analysis of RdRps from nine viruses of the family Reoviridae and polyhedrin from several characterized cypoviruses indicate that DpCPV-Hn is a type 1 cypovirus, more related to Bombyx mori cypovirus (BmCPV) than to other cypovirus species
引文
1. 中山大学生物系昆虫专业、电子显微镜室. 1976. 中山大学学报. 3：11.
2. 田静等. 1999.草鱼出血病病毒基因组基因组的cDNA合成、克隆、及部分序列分析. 中国病毒学. 14（1）：87.
3. 陈昌洁. 1990. 松毛虫综合管理. 中国林业出版社.
4. 陈昌洁. 1988. 赤松毛虫质型多角体病毒的引进和利用研究. 林业科学研究. 11, 14.
5. 陈昌洁. 1990. 利用棉铃虫为宿主增殖松毛虫质型多角体病毒.林业科学研究. 3：263-265.
6. 陈延伟等. 1979. 微生物学报. 19（3）：292-296.
7. 陈延伟等. 1979. 赤松毛虫的一种新的质型多角体病毒的研究. 微生物学报. 19（3）：292.
8. 华中师院生物系昆虫病毒研究室. 1978. 昆虫学报. 21（2）：133-136.
9. 吕鸿声. 1982. 昆虫病毒与昆虫病毒病. 科学出版社.
10. 吕鸿声. 1998. 昆虫病毒分子生物学. 中国农业科技出版社.
11. 许耀先等. 1999. 病毒命名与分类系统研究进展.中国病毒学. 14（3）：190.
12. 杜建宇等. 2001. 马尾松毛虫质型多角体病毒多角体蛋白基因的cDNA克隆及序列分析. 中国病毒学. 16（4）：350.
13. 沈中建等. 1994. 马尾松毛虫质型多角体病毒在昆虫细胞系中离体增殖的研究. 病毒学报. 10（1）：44.
14. 苏德明、乐云仙、陈梅琴、杨琰云. 1978. 复旦大学学报. 1：74-78.
15. 余学奎等. 1998. 家蚕质型多角体病毒（BmCPV）的结构研究. 电子显微镜学报. 17（4）：389.
16. 余学奎等. 1999. 家蚕质型多角体病毒（BmCPV）结构研究. 生物化学与生物物理学报. 31（5）：563.
17. 金冬雁等译. 1992. 分子克隆实验指南. 第二版. 科学出版社.
18. 周小毛等. 1993. 马尾松毛虫质型多角体病毒替代宿主的研究. 华南农业大学学报. 14（1），114-121.
19. 侯云德. 1990. 分子病毒学. 学苑出版社.
20. 陶粮等. 1998. 七株松毛虫质型多角体病毒RNA基因图谱比较研究. 林业科学. 24（1）：29.
21. 曹诒孙、钱元骏. 1957. 蚕业科学通讯. 1：11-14.
22. 曾陈湘等 . 1997. 利用替代宿主生产马尾松毛虫质型多角体病毒的研究. 广东林业科技. 13（4）: 6-13.
23．Anzola, J.V., Xu, Z., Asamizu, T., Nuss, D.L., 1987. Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Pro NAS. 84, 8301-8305.
24．Arella, M., Lavallee, C., Belloncik, S., Furuichi, Y., 1988. Molecular cloning and characteration of cytoplasmic polyhedrosis virus polyhedrin and a viable deletion mutant gene. J. Virol. 62, 211-217
25．Attoui, H., Billoir, F., Biagini, P., Micco, P., Lamballerie, X., 2000. Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J. Gen. Virol. 81,1507-1515.
26．Azuhata, F., Uyeda, I., Shikata, E., 1992. Conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus. J. Gen. Virol. 73, 1593-1595.
27． Belloncik, S., 1989. Cytoplasmic polyhedrosis viruses-Reoviridae. Adv. Virus Res. 37, 173-209.
28. Belloncik, S and Mori, H., 1998. Cypoviruses, P. 337-369. In L.K.Miller and L. A. Bauled., The insect viruses. Plenum publishing corporation, New York, N. Y.Belloncik, S., 1989. Cytoplasmic polyhedrosis viruses-Reoviridae. Adv. Virus. Res. 37, 173-209.
29. Belloncik, S. J., Liu, D.S., Arella, M., 1996. Identification and characteration of a new cypovirus, type 14, isolated from Heliothis armigera. J. Invertebr. Pathol. 67, 41-47.
30． Brentano, L., Noah, D. L., Brown, E.G and Sherry, B., 1998. The reovirus protein u2, encoded by the M1 gene, is an RNA-binding protein. J. Virol. 72, 8354-8357.
31. Cashdollar, L.M., Esparza, J., Hudson, G.R., 1982. Cloning the double stranded RNA genes of reovirus: sequence of cloned S2 gene. Proc Natl Acad Sci USA. 79, 7644-7648
32． Clewey, J.P., 1998. A user's guide to producing and interpreting tree diagram in taxonomy and phylogenetics. Communicable Disease and Public Health. 30, 201-205.
33. Desselberger, U. et al., 1980. The 3' and 5' terminal sequence of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene. 8, 315.
34． Dryden, K. A., Farsetta, D. L., Wang, G., Keegan, J. M., Fields, B. N., Baker, T. S., Nibert, M.L., 1998. Internal structures containing transcriptase-related proteins in top component particles of mammalian orthoreovirus. Virology. 245, 33-46.
35. Du, J.Y., Zhang, J.M., Guo, H.T., 2001. Molecular cloning and sequence analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus polyhedrin gene. Virologica Sinica. 16,350-354.
36． Echeverry, F., Bergeron, J., Kaupp, W., Guertin, C., Arella, M., 1997. Sequence analysis and expression of the polyhedrin gene of Choristoneura fumiferana cytoplasmic polyhedrosis virus (CfCPV). Gene. 198, 399-406.
37． Estes, M.K., Cohen, J., 1989. Rotavirus gene structure and function. Microbiol. Rev. 53,410-449.
38． Fossiez, F., Belloncik, S., Arella, M., 1989. Nucleotide sequence of the polyhedrin gene of Euxoa scandens cytoplasmic polyhedrosis virus(EsCPV). Virology. 169, 462-465.
39. Fouilaud M, Morel G. 1994. Characterisation of cytoplasmic and nuclear polyhedrosis viruses recovered from the nest of Polistes hebraeus F. J. Invertebr. Pathol. 42, 401.
40． Francki, R.I.B., Faiquet, C.M., Knudson, D.L., Brown, F., 1991. Classification and nonenclature of viruses. Fifth Report of the International Committee on Taxonomy of viruses. Arch Virol Suppl 2 Springer Vienna.
41． Galinski, M.S., Yu, Y., Heminway, B.R., Beaudreau, G.S., 1994. Analysis of the C-polyhedrin genes from different geographical isolates of a type 5 cytoplasmic polyhedrosis virus. J. Gen. Virol. 75, 1969-1974.
42． Hagiwara, K., Kobayashi, J., Tomita, M., Yoshimura, T., 2001. Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1. Arch. Virol. 146, 181-187.
43． Hagiwarw, K., Matsumoto, T., 2000. Nucleotide sequences of genome segments 6 and 7 of Bombyx mori cypovirus1,encoding the viral structural proteins V4 and V5, respectively. J. Gen. Virol. 61, 1143-1147.
44． Hagiwara, K., Rao, S., .Scott, S. W., Carner, G.R., 2002. Nucleotide sequences of segments 1,3 and 4 of the genome of Bombyx mori cypovirus 1 encoding putative capsid proteins VP1,VP3 and VP4, respectively. J. Gen. Virol. 83, 1477-1482.
45． Hagiwara, K., Tomita, M., Kobayashi, J., Miyajima, S., Yoshimura, T., 1998. Nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 8. Biochem. Biophys. Res. Commun. 247, 549-553.
46． Hagiwara, K., Tomita, M., Nakai, K., Kobayashi, J., Miyajima, S., Yoshimura, T., 1998. Determination of the nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells. J. Virol . 72, 5762-5768.
47． Hatta, T and Francki, R.I.B., 1977. Morphology of Fiji disease virus. Virology. 76, 797-807.
48. Hayashi, Y., Bird, F.T., 1970. The isolation of cytoplasmic polyhedrosis virus from the white-marked tussock moth. Canadian Journal of Microbiology. 6,695-701.
49． Hill, C.l., Booth, T.F., Prasad B.V.V., Grimes, J.M., Mertens, P.P.C., Sutton, G.C., Stuart, D.I., 1999. The structure of a cypovirus and the functional organization of dsRNA virus. Nature Struct Biol. 6,565-568.
50． Hoimes, I.H., Boccardo, G., Estes, M.K., Furuichi, M.K., Hoshino, Y., Joklik, W.K., McCrae, M., Mertens, P.P.C., Milne, R.G., Samal, K.S.K., Shikata, E., Winton, J.R., Uyeda, I., Nuss, D.L., 1994. Family Reoviridae, pp208-239.
51． Ikeda, K., Nagaoka, S., Winkler, S., Kotani, K., Yagi, H., Nakanishi, K., Miyajima, S., Kobayashi, J., Mori, H., 2001. Molecular characteration of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4. J. Virol. 75, 988-995.
52. James, E.A. et al . 1982. Sequence at both terminai of 10 genes of reovirus serotype 3(Strain Dearing). Virology. 121:307.
53． Kuchino, Y., Nishimura, S., Smith, R.E., Furuichi, Y., 1982. Homologous terminal sequences in the double-stranded RNA genome segments of cytoplasmic polyhedrosis virus of the silkworm Bombyx mori. J. Virol. 44, 538-543.
54． Kudo, H., Uyeda, I., Shikata, E., 1991. Viruses in the Phytoreovirus genus of the Reoviridae family have the same conserved terminal sequences. J. Gen. Virol. 72, 2857-2866.
55． Kumar, S., Tamura, K., Nei, M., 1993. Molecular Evolutionary Genetic Analysis, Version 1.01. Pennsylvania State University.
56. Lewandowski, L. J., Traynor, B. L., 1972. Comparison of the strucure and polypeptide composition of three double-stranded ribonucleic acid-containing viruses (Diplornaviruses): cytoplasmic polyhedrosis, wound tumor virus, and reovirus. J. Virol. 10, 1053-1070.
57． Liu, R.Z., 1992. Studies on fine structure and some physico-chemical characteristics of a cytoplasmic polyhedrosis virus from Dendnolimus punctatus Wensh- anensis. Virologica Sinica. 17, 69-73.
58． Lambden, P. R., Cooke, S. J., Caul, E. O., Clarke, I. N., 1992. Cloning of noncultivatable human rotavirus by single primer amplification. J. Virol. 66, 1817-1822.
59． MaCrae, M.A., Mertens, P.P.C., 1983. In vitro translation studies on and RNA coding assignments for cytoplasmic polyhedrosis viruses. In: Compans RW, Bishop DHI(eds) Double-stranded RNA viruses. Elsevier, New York, pp 35-41
60． Matthews, R.E.F., 1982. Classification and nomemclature of viruses. Intervirology. pp171-190.
61． Mattion, N.M., Harnish, E.C., Crowley, J.C., Reilly, P.A., 1996. Foot-and-mouth disease virus 2A protease mediates cleavage in attenuated sabin 3 poliovirus vectors engineered for delivery of foreign antigen. J. Virol. 70, 8124-8127.
62. Mertens, P.P.C. et al. 1999. A comparison of six cypovirus isolates by cross-hybridization
of their dsRNA genome segments. Arch. Virol. 144(3), 561.
63． Mertens, P. P. C., Crook, N., Rubinstein, R., Pedley, S., Payne, C., 1989. Cytoplasmic polyhedrosis virus classification by electropherotype: validation by serological analyses and agarose gel electrophoresis. J Gen. Virol. 70, 173-185
64． Mertens, P.P.S., Arella, M., Attoui, H., Bellonik, S., Bergoin, M et al., 2000. Reoviridae. In Van Regenmortel, M.H.V., Fauquet, C.M., Bioshop, D.H.L., Calisher, C.H., Carsten, E.B., Estes, M.K., Lemon, S.M., Maniloff, J., Mayo, M.A., McGeoch, D.J., Pringle, C.R., Wickner, R.B (eds) Virus Taxonomy. Seventh Report of the International Committen for the Taxonomy of Viruses. Academic Press, NewYork, pp395-480.
65． Mori, H., Minobe, Y., Sasaki, T., Kawase, S., 1989. Nucleotide sequence of the polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus A strain with nuclear localization of polyhedra. J. Gen. Virol. 70, 1885-1888.
66. Nakazawa, H.F. et al. 1996. Effect of mutation on the intracellar localization of Bonbyx mori cytoplasmic polyhedrosis virus polyhedrin. J.Gen.Virol. 77:147.
67． Noda, H., Nakashima, N., Omura, T., 1994. Cloning of the Nilaparvata lugens reovirus genome: conserved terminal nucleotide sequences and nucleotide sequence of genome segment S10. J. Gen. Virol. 75, 221-225.
68． Page, R.D., 1996. Treeview: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences. 12, 357-358.
69. Patten, J.T., Spencer,E., 2000. Genome replication of packaging of segmented double-stranded RNA viruses. Virology. 277,217-225.
70. Patton, J. T., 1995. Structure and function of the rotavirus RNA-binding proteins. J. Gen. Virol. 76, 2633-2644.
71. Patton, J. T., 2000. Genome replication and packaging of segmented double-stranded RNA viruses. Virology. 277, 217-225.
72. Payne, C.C., Rivers, C.F., 1976. A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. J. Gen. Virol. 33, 71-81.
73. Payne, C.C., Serafin, M.P., Pilley, B., 1977. The properties of two recent isolates of cytoplasmic polyhedrosis viruses. Intervirology. 8, 155-163.
74. Qanungo, K.R., Kundu, S.C., Ghosh, A.K., 2000. Characterization of cypovirus isolates from tropical and temperate Indian Saturniidaee silkworms. Acta. Virol. 44(6), 349-357.
75． Qanungo, K.R., Kundu, S.C., Mullins, J.I., Ghosh, A.K., 2002. Molecular cloning and characterization of Antheraea mylitta cytoplasmic polyhedrosis virus genome segment 9. J. Gen. Virol. 83, 1483-1491.
76. Ryan, M.D., King, A.M.Q., Thomas, G.P., 1991. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence. J. Gen. Virol. 72, 2727-2732.
77. Smith, K.M. and Wychoff, R.W., 1950. Nature. 166, 861.
78. Smith, K.M. and Xeros, N., 1953. Parasitology. 43, 178-185.
79． Suzuki, N., 1995. Molecular analysis of rice dwart virus genome. Semin. Virol. 6, 89-95.
80. Xeros, N., 1952. Nature. 170, 1073.
81. Upadhyaya, N.M., Yang, M., Kositratana, W., Ghosh, A., Waterhouse, P.M., 1995. Molecular analysis of rice ragged stunt Oryzavirus segment 9 and sequence conservation among isolates from Thailand and India. Arch. Virol. 140, 1945-1956.
82. Xia, D. et al. X-ray power pattern analysis of cytoplasmic polyhedrosis virus of
silkworm Bombyx mori. J. Virol. 44(2), 538.
83． Zhang,H., Zhang,J., Yu,X., Lu,X., Zhang,Q., Jakana, J., Chen, D.H., Zhang,X., and Zhou, Z.H., 1999. Visulization of protein-RNA interactions in cytoplasmic polyhedrosis virus. J. Virol. 73,1624-1629.
84． Zhong, B.X., 2001. NTP binding proteins of Bombyx mori cytoplasmic polyhedrosis virus. Science of Sericulture. 27(1), 38-41.
2. 田静等. 1999.草鱼出血病病毒基因组基因组的cDNA合成、克隆、及部分序列分析. 中国病毒学. 14（1）：87.
3. 陈昌洁. 1990. 松毛虫综合管理. 中国林业出版社.
4. 陈昌洁. 1988. 赤松毛虫质型多角体病毒的引进和利用研究. 林业科学研究. 11, 14.
5. 陈昌洁. 1990. 利用棉铃虫为宿主增殖松毛虫质型多角体病毒.林业科学研究. 3：263-265.
6. 陈延伟等. 1979. 微生物学报. 19（3）：292-296.
7. 陈延伟等. 1979. 赤松毛虫的一种新的质型多角体病毒的研究. 微生物学报. 19（3）：292.
8. 华中师院生物系昆虫病毒研究室. 1978. 昆虫学报. 21（2）：133-136.
9. 吕鸿声. 1982. 昆虫病毒与昆虫病毒病. 科学出版社.
10. 吕鸿声. 1998. 昆虫病毒分子生物学. 中国农业科技出版社.
11. 许耀先等. 1999. 病毒命名与分类系统研究进展.中国病毒学. 14（3）：190.
12. 杜建宇等. 2001. 马尾松毛虫质型多角体病毒多角体蛋白基因的cDNA克隆及序列分析. 中国病毒学. 16（4）：350.
13. 沈中建等. 1994. 马尾松毛虫质型多角体病毒在昆虫细胞系中离体增殖的研究. 病毒学报. 10（1）：44.
14. 苏德明、乐云仙、陈梅琴、杨琰云. 1978. 复旦大学学报. 1：74-78.
15. 余学奎等. 1998. 家蚕质型多角体病毒（BmCPV）的结构研究. 电子显微镜学报. 17（4）：389.
16. 余学奎等. 1999. 家蚕质型多角体病毒（BmCPV）结构研究. 生物化学与生物物理学报. 31（5）：563.
17. 金冬雁等译. 1992. 分子克隆实验指南. 第二版. 科学出版社.
18. 周小毛等. 1993. 马尾松毛虫质型多角体病毒替代宿主的研究. 华南农业大学学报. 14（1），114-121.
19. 侯云德. 1990. 分子病毒学. 学苑出版社.
20. 陶粮等. 1998. 七株松毛虫质型多角体病毒RNA基因图谱比较研究. 林业科学. 24（1）：29.
21. 曹诒孙、钱元骏. 1957. 蚕业科学通讯. 1：11-14.
22. 曾陈湘等 . 1997. 利用替代宿主生产马尾松毛虫质型多角体病毒的研究. 广东林业科技. 13（4）: 6-13.
23．Anzola, J.V., Xu, Z., Asamizu, T., Nuss, D.L., 1987. Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Pro NAS. 84, 8301-8305.
24．Arella, M., Lavallee, C., Belloncik, S., Furuichi, Y., 1988. Molecular cloning and characteration of cytoplasmic polyhedrosis virus polyhedrin and a viable deletion mutant gene. J. Virol. 62, 211-217
25．Attoui, H., Billoir, F., Biagini, P., Micco, P., Lamballerie, X., 2000. Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J. Gen. Virol. 81,1507-1515.
26．Azuhata, F., Uyeda, I., Shikata, E., 1992. Conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus. J. Gen. Virol. 73, 1593-1595.
27． Belloncik, S., 1989. Cytoplasmic polyhedrosis viruses-Reoviridae. Adv. Virus Res. 37, 173-209.
28. Belloncik, S and Mori, H., 1998. Cypoviruses, P. 337-369. In L.K.Miller and L. A. Bauled., The insect viruses. Plenum publishing corporation, New York, N. Y.Belloncik, S., 1989. Cytoplasmic polyhedrosis viruses-Reoviridae. Adv. Virus. Res. 37, 173-209.
29. Belloncik, S. J., Liu, D.S., Arella, M., 1996. Identification and characteration of a new cypovirus, type 14, isolated from Heliothis armigera. J. Invertebr. Pathol. 67, 41-47.
30． Brentano, L., Noah, D. L., Brown, E.G and Sherry, B., 1998. The reovirus protein u2, encoded by the M1 gene, is an RNA-binding protein. J. Virol. 72, 8354-8357.
31. Cashdollar, L.M., Esparza, J., Hudson, G.R., 1982. Cloning the double stranded RNA genes of reovirus: sequence of cloned S2 gene. Proc Natl Acad Sci USA. 79, 7644-7648
32． Clewey, J.P., 1998. A user's guide to producing and interpreting tree diagram in taxonomy and phylogenetics. Communicable Disease and Public Health. 30, 201-205.
33. Desselberger, U. et al., 1980. The 3' and 5' terminal sequence of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene. 8, 315.
34． Dryden, K. A., Farsetta, D. L., Wang, G., Keegan, J. M., Fields, B. N., Baker, T. S., Nibert, M.L., 1998. Internal structures containing transcriptase-related proteins in top component particles of mammalian orthoreovirus. Virology. 245, 33-46.
35. Du, J.Y., Zhang, J.M., Guo, H.T., 2001. Molecular cloning and sequence analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus polyhedrin gene. Virologica Sinica. 16,350-354.
36． Echeverry, F., Bergeron, J., Kaupp, W., Guertin, C., Arella, M., 1997. Sequence analysis and expression of the polyhedrin gene of Choristoneura fumiferana cytoplasmic polyhedrosis virus (CfCPV). Gene. 198, 399-406.
37． Estes, M.K., Cohen, J., 1989. Rotavirus gene structure and function. Microbiol. Rev. 53,410-449.
38． Fossiez, F., Belloncik, S., Arella, M., 1989. Nucleotide sequence of the polyhedrin gene of Euxoa scandens cytoplasmic polyhedrosis virus(EsCPV). Virology. 169, 462-465.
39. Fouilaud M, Morel G. 1994. Characterisation of cytoplasmic and nuclear polyhedrosis viruses recovered from the nest of Polistes hebraeus F. J. Invertebr. Pathol. 42, 401.
40． Francki, R.I.B., Faiquet, C.M., Knudson, D.L., Brown, F., 1991. Classification and nonenclature of viruses. Fifth Report of the International Committee on Taxonomy of viruses. Arch Virol Suppl 2 Springer Vienna.
41． Galinski, M.S., Yu, Y., Heminway, B.R., Beaudreau, G.S., 1994. Analysis of the C-polyhedrin genes from different geographical isolates of a type 5 cytoplasmic polyhedrosis virus. J. Gen. Virol. 75, 1969-1974.
42． Hagiwara, K., Kobayashi, J., Tomita, M., Yoshimura, T., 2001. Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1. Arch. Virol. 146, 181-187.
43． Hagiwarw, K., Matsumoto, T., 2000. Nucleotide sequences of genome segments 6 and 7 of Bombyx mori cypovirus1,encoding the viral structural proteins V4 and V5, respectively. J. Gen. Virol. 61, 1143-1147.
44． Hagiwara, K., Rao, S., .Scott, S. W., Carner, G.R., 2002. Nucleotide sequences of segments 1,3 and 4 of the genome of Bombyx mori cypovirus 1 encoding putative capsid proteins VP1,VP3 and VP4, respectively. J. Gen. Virol. 83, 1477-1482.
45． Hagiwara, K., Tomita, M., Kobayashi, J., Miyajima, S., Yoshimura, T., 1998. Nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 8. Biochem. Biophys. Res. Commun. 247, 549-553.
46． Hagiwara, K., Tomita, M., Nakai, K., Kobayashi, J., Miyajima, S., Yoshimura, T., 1998. Determination of the nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells. J. Virol . 72, 5762-5768.
47． Hatta, T and Francki, R.I.B., 1977. Morphology of Fiji disease virus. Virology. 76, 797-807.
48. Hayashi, Y., Bird, F.T., 1970. The isolation of cytoplasmic polyhedrosis virus from the white-marked tussock moth. Canadian Journal of Microbiology. 6,695-701.
49． Hill, C.l., Booth, T.F., Prasad B.V.V., Grimes, J.M., Mertens, P.P.C., Sutton, G.C., Stuart, D.I., 1999. The structure of a cypovirus and the functional organization of dsRNA virus. Nature Struct Biol. 6,565-568.
50． Hoimes, I.H., Boccardo, G., Estes, M.K., Furuichi, M.K., Hoshino, Y., Joklik, W.K., McCrae, M., Mertens, P.P.C., Milne, R.G., Samal, K.S.K., Shikata, E., Winton, J.R., Uyeda, I., Nuss, D.L., 1994. Family Reoviridae, pp208-239.
51． Ikeda, K., Nagaoka, S., Winkler, S., Kotani, K., Yagi, H., Nakanishi, K., Miyajima, S., Kobayashi, J., Mori, H., 2001. Molecular characteration of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4. J. Virol. 75, 988-995.
52. James, E.A. et al . 1982. Sequence at both terminai of 10 genes of reovirus serotype 3(Strain Dearing). Virology. 121:307.
53． Kuchino, Y., Nishimura, S., Smith, R.E., Furuichi, Y., 1982. Homologous terminal sequences in the double-stranded RNA genome segments of cytoplasmic polyhedrosis virus of the silkworm Bombyx mori. J. Virol. 44, 538-543.
54． Kudo, H., Uyeda, I., Shikata, E., 1991. Viruses in the Phytoreovirus genus of the Reoviridae family have the same conserved terminal sequences. J. Gen. Virol. 72, 2857-2866.
55． Kumar, S., Tamura, K., Nei, M., 1993. Molecular Evolutionary Genetic Analysis, Version 1.01. Pennsylvania State University.
56. Lewandowski, L. J., Traynor, B. L., 1972. Comparison of the strucure and polypeptide composition of three double-stranded ribonucleic acid-containing viruses (Diplornaviruses): cytoplasmic polyhedrosis, wound tumor virus, and reovirus. J. Virol. 10, 1053-1070.
57． Liu, R.Z., 1992. Studies on fine structure and some physico-chemical characteristics of a cytoplasmic polyhedrosis virus from Dendnolimus punctatus Wensh- anensis. Virologica Sinica. 17, 69-73.
58． Lambden, P. R., Cooke, S. J., Caul, E. O., Clarke, I. N., 1992. Cloning of noncultivatable human rotavirus by single primer amplification. J. Virol. 66, 1817-1822.
59． MaCrae, M.A., Mertens, P.P.C., 1983. In vitro translation studies on and RNA coding assignments for cytoplasmic polyhedrosis viruses. In: Compans RW, Bishop DHI(eds) Double-stranded RNA viruses. Elsevier, New York, pp 35-41
60． Matthews, R.E.F., 1982. Classification and nomemclature of viruses. Intervirology. pp171-190.
61． Mattion, N.M., Harnish, E.C., Crowley, J.C., Reilly, P.A., 1996. Foot-and-mouth disease virus 2A protease mediates cleavage in attenuated sabin 3 poliovirus vectors engineered for delivery of foreign antigen. J. Virol. 70, 8124-8127.
62. Mertens, P.P.C. et al. 1999. A comparison of six cypovirus isolates by cross-hybridization
of their dsRNA genome segments. Arch. Virol. 144(3), 561.
63． Mertens, P. P. C., Crook, N., Rubinstein, R., Pedley, S., Payne, C., 1989. Cytoplasmic polyhedrosis virus classification by electropherotype: validation by serological analyses and agarose gel electrophoresis. J Gen. Virol. 70, 173-185
64． Mertens, P.P.S., Arella, M., Attoui, H., Bellonik, S., Bergoin, M et al., 2000. Reoviridae. In Van Regenmortel, M.H.V., Fauquet, C.M., Bioshop, D.H.L., Calisher, C.H., Carsten, E.B., Estes, M.K., Lemon, S.M., Maniloff, J., Mayo, M.A., McGeoch, D.J., Pringle, C.R., Wickner, R.B (eds) Virus Taxonomy. Seventh Report of the International Committen for the Taxonomy of Viruses. Academic Press, NewYork, pp395-480.
65． Mori, H., Minobe, Y., Sasaki, T., Kawase, S., 1989. Nucleotide sequence of the polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus A strain with nuclear localization of polyhedra. J. Gen. Virol. 70, 1885-1888.
66. Nakazawa, H.F. et al. 1996. Effect of mutation on the intracellar localization of Bonbyx mori cytoplasmic polyhedrosis virus polyhedrin. J.Gen.Virol. 77:147.
67． Noda, H., Nakashima, N., Omura, T., 1994. Cloning of the Nilaparvata lugens reovirus genome: conserved terminal nucleotide sequences and nucleotide sequence of genome segment S10. J. Gen. Virol. 75, 221-225.
68． Page, R.D., 1996. Treeview: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences. 12, 357-358.
69. Patten, J.T., Spencer,E., 2000. Genome replication of packaging of segmented double-stranded RNA viruses. Virology. 277,217-225.
70. Patton, J. T., 1995. Structure and function of the rotavirus RNA-binding proteins. J. Gen. Virol. 76, 2633-2644.
71. Patton, J. T., 2000. Genome replication and packaging of segmented double-stranded RNA viruses. Virology. 277, 217-225.
72. Payne, C.C., Rivers, C.F., 1976. A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. J. Gen. Virol. 33, 71-81.
73. Payne, C.C., Serafin, M.P., Pilley, B., 1977. The properties of two recent isolates of cytoplasmic polyhedrosis viruses. Intervirology. 8, 155-163.
74. Qanungo, K.R., Kundu, S.C., Ghosh, A.K., 2000. Characterization of cypovirus isolates from tropical and temperate Indian Saturniidaee silkworms. Acta. Virol. 44(6), 349-357.
75． Qanungo, K.R., Kundu, S.C., Mullins, J.I., Ghosh, A.K., 2002. Molecular cloning and characterization of Antheraea mylitta cytoplasmic polyhedrosis virus genome segment 9. J. Gen. Virol. 83, 1483-1491.
76. Ryan, M.D., King, A.M.Q., Thomas, G.P., 1991. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence. J. Gen. Virol. 72, 2727-2732.
77. Smith, K.M. and Wychoff, R.W., 1950. Nature. 166, 861.
78. Smith, K.M. and Xeros, N., 1953. Parasitology. 43, 178-185.
79． Suzuki, N., 1995. Molecular analysis of rice dwart virus genome. Semin. Virol. 6, 89-95.
80. Xeros, N., 1952. Nature. 170, 1073.
81. Upadhyaya, N.M., Yang, M., Kositratana, W., Ghosh, A., Waterhouse, P.M., 1995. Molecular analysis of rice ragged stunt Oryzavirus segment 9 and sequence conservation among isolates from Thailand and India. Arch. Virol. 140, 1945-1956.
82. Xia, D. et al. X-ray power pattern analysis of cytoplasmic polyhedrosis virus of
silkworm Bombyx mori. J. Virol. 44(2), 538.
83． Zhang,H., Zhang,J., Yu,X., Lu,X., Zhang,Q., Jakana, J., Chen, D.H., Zhang,X., and Zhou, Z.H., 1999. Visulization of protein-RNA interactions in cytoplasmic polyhedrosis virus. J. Virol. 73,1624-1629.
84． Zhong, B.X., 2001. NTP binding proteins of Bombyx mori cytoplasmic polyhedrosis virus. Science of Sericulture. 27(1), 38-41.