扇贝、对虾基因组BAC文库构建及其重要功能基因的初步筛选和分析
|
摘要
基因组大片段BAC文库是进行生物遗传学和基因组学研究必不可少的基础工具。为了深入开展栉孔扇贝(Chlamys farreri)和凡纳滨对虾(Litopenaeus vannamei)基因组学研究、阐明其基因组的结构与功能、图位克隆重要功能基因、构建高密度物理图谱并最终实现与已有遗传连锁图的整合,本研究在植物基因组BAC文库构建技术的基础上,针对海洋生物的特点进行了大胆的改革与尝试,最终成功构建了C. ferrari和L. vannamei两种重要海水养殖动物的基因组BAC文库。
本文构建的栉孔扇贝基因组BAC文库,由BamHI文库和MboI文库构成。其中BamHI文库含有73,728个BAC克隆,空载率约为1%,平均插入片段约为110 kb,覆盖栉孔扇贝单倍体基因组约8倍;MboI文库共有7,680个克隆组成,平均插入片段大小约为145 kb,插入率为100%,覆盖栉孔扇贝单倍体基因组约1.1倍。两个栉孔扇贝基因组BAC文库共由81,408个克隆组成,平均插入片段约为113 kb,覆盖率约为栉孔扇贝单倍体基因组大小的9.1倍。
将栉孔扇贝基因组BAC文库的192个384微孔培养板中的73,728个BAC克隆以4 x 4点阵形式制备了高密度DNA薄膜,用于对感兴趣的基因及DNA序列的筛选。高密度DNA薄膜的覆盖率约为栉孔扇贝单倍体基因组的8.3倍。针对栉孔扇贝先天免疫系统通路的6个重要功能基因,根据栉孔扇贝cDNA序列以及异缘物种DNA序列设计了Overgo探针。利用Overgo探针对高密度DNA薄膜杂交筛选的结果显示,平均每个基因检测到7.3个潜在阳性克隆。
本研究所构建的凡纳滨对虾基因组HindIII酶切BAC文库共有102,528个BAC克隆,存放于267个384微孔培养板中,平均插入片段大小约为101 kb,空载率约为5%,覆盖L. vannamei单倍体基因组约5倍。将其中240个384微孔培养板中的92,160个BAC克隆以4 x 4的矩阵排列形式制作了5张高密度凡纳滨对虾DNA薄膜,约覆盖整个对虾单倍体基因组的4.5倍。针对6个与对虾免疫、生殖生理有关的重要功能基因设计了Overgo探针,杂交筛选出20个阳性克隆,平均每个基因有3.3个潜在阳性克隆。
以上筛选结果不仅为进一步研究这些功能基因的结构与功能、表达与调控,揭示它们在扇贝和对虾以及其他近缘种的免疫系统、抗逆和生殖生理过程中的作用机理打下了基础,同时也间接验证了栉孔扇贝和凡纳滨对虾基因组BAC文库将成为基因筛选、基因的结构与功能分析、基因图位克隆、物理图谱构建以及大规模全基因组测序等方面的有力工具。
Large-insert bacterial artificial chromosome (BAC) libraries are necessary for advanced genetics and genomics research. To facilitate gene cloning and characterization, genome analysis and physical mapping of Zhikong Scallop, Chlamys farreri Jones et Preston and Pacific white shrimp, Litopenaeus vannamei, two BAC libraries were constructed from nuclear DNA of the two species, respectively. The scallop BAC libraries were constructed in the BamHI and MboI sites of the vector pECBAC1, respectively. The BamHI library consists of 73,728 clones, and approximately 99 % of the clones contain scallop nuclear DNA inserts with an average size of 110 kb, covering 8.0 x haploid genome equivalents. Similarly, the MboI library consists of 7,680 clones, with an average insert size of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 x. The combined libraries collectively contain a total of 81,408 BAC clones arrayed in 212 384-well microtiter plates, representing 9.1 x haploid genome equivalents and having a probability of greater than 99% of discovering at least one positive clone with a single-copy sequence. High-density clone filters prepared from a subset of the two libraries were screened with nine pairs of Overgos designed from the cDNA or DNA sequences of six genes involved in the innate immune system of mollusks. Positive clones were identified for every gene, with an average of 7.3 BAC clones per gene probe. These results suggest that the two scallop BAC libraries provide useful tools for gene cloning, genome physical mapping and large-scale sequencing in the species.
The shrimp BAC libraries were constructed in the HindIII site of the vectors pECBAC1 and pCLD04541, respectively. The combined libraries consists of 102,528 clones arrayed in 267 384-well microtiter plates, and approximately 95 % of the clones contain shrimp nuclear DNA inserts with an average size of 101 kb, covering 5.0 x haploid genome equivalents. High-density clone filters prepared from a subset of the pECBAC1 library were screened with Overgos designed from the cDNA sequences of six genes involved in immune system and sex determination related factor. Positive clones were identified for every gene, with an average of 3.3 BAC clones per gene probe. These results suggest that the shrimp BAC libraries provide useful tools for gene cloning, genome physical mapping and large-scale sequencing in the species.
本文构建的栉孔扇贝基因组BAC文库,由BamHI文库和MboI文库构成。其中BamHI文库含有73,728个BAC克隆,空载率约为1%,平均插入片段约为110 kb,覆盖栉孔扇贝单倍体基因组约8倍;MboI文库共有7,680个克隆组成,平均插入片段大小约为145 kb,插入率为100%,覆盖栉孔扇贝单倍体基因组约1.1倍。两个栉孔扇贝基因组BAC文库共由81,408个克隆组成,平均插入片段约为113 kb,覆盖率约为栉孔扇贝单倍体基因组大小的9.1倍。
将栉孔扇贝基因组BAC文库的192个384微孔培养板中的73,728个BAC克隆以4 x 4点阵形式制备了高密度DNA薄膜,用于对感兴趣的基因及DNA序列的筛选。高密度DNA薄膜的覆盖率约为栉孔扇贝单倍体基因组的8.3倍。针对栉孔扇贝先天免疫系统通路的6个重要功能基因,根据栉孔扇贝cDNA序列以及异缘物种DNA序列设计了Overgo探针。利用Overgo探针对高密度DNA薄膜杂交筛选的结果显示,平均每个基因检测到7.3个潜在阳性克隆。
本研究所构建的凡纳滨对虾基因组HindIII酶切BAC文库共有102,528个BAC克隆,存放于267个384微孔培养板中,平均插入片段大小约为101 kb,空载率约为5%,覆盖L. vannamei单倍体基因组约5倍。将其中240个384微孔培养板中的92,160个BAC克隆以4 x 4的矩阵排列形式制作了5张高密度凡纳滨对虾DNA薄膜,约覆盖整个对虾单倍体基因组的4.5倍。针对6个与对虾免疫、生殖生理有关的重要功能基因设计了Overgo探针,杂交筛选出20个阳性克隆,平均每个基因有3.3个潜在阳性克隆。
以上筛选结果不仅为进一步研究这些功能基因的结构与功能、表达与调控,揭示它们在扇贝和对虾以及其他近缘种的免疫系统、抗逆和生殖生理过程中的作用机理打下了基础,同时也间接验证了栉孔扇贝和凡纳滨对虾基因组BAC文库将成为基因筛选、基因的结构与功能分析、基因图位克隆、物理图谱构建以及大规模全基因组测序等方面的有力工具。
Large-insert bacterial artificial chromosome (BAC) libraries are necessary for advanced genetics and genomics research. To facilitate gene cloning and characterization, genome analysis and physical mapping of Zhikong Scallop, Chlamys farreri Jones et Preston and Pacific white shrimp, Litopenaeus vannamei, two BAC libraries were constructed from nuclear DNA of the two species, respectively. The scallop BAC libraries were constructed in the BamHI and MboI sites of the vector pECBAC1, respectively. The BamHI library consists of 73,728 clones, and approximately 99 % of the clones contain scallop nuclear DNA inserts with an average size of 110 kb, covering 8.0 x haploid genome equivalents. Similarly, the MboI library consists of 7,680 clones, with an average insert size of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 x. The combined libraries collectively contain a total of 81,408 BAC clones arrayed in 212 384-well microtiter plates, representing 9.1 x haploid genome equivalents and having a probability of greater than 99% of discovering at least one positive clone with a single-copy sequence. High-density clone filters prepared from a subset of the two libraries were screened with nine pairs of Overgos designed from the cDNA or DNA sequences of six genes involved in the innate immune system of mollusks. Positive clones were identified for every gene, with an average of 7.3 BAC clones per gene probe. These results suggest that the two scallop BAC libraries provide useful tools for gene cloning, genome physical mapping and large-scale sequencing in the species.
The shrimp BAC libraries were constructed in the HindIII site of the vectors pECBAC1 and pCLD04541, respectively. The combined libraries consists of 102,528 clones arrayed in 267 384-well microtiter plates, and approximately 95 % of the clones contain shrimp nuclear DNA inserts with an average size of 101 kb, covering 5.0 x haploid genome equivalents. High-density clone filters prepared from a subset of the pECBAC1 library were screened with Overgos designed from the cDNA sequences of six genes involved in immune system and sex determination related factor. Positive clones were identified for every gene, with an average of 3.3 BAC clones per gene probe. These results suggest that the shrimp BAC libraries provide useful tools for gene cloning, genome physical mapping and large-scale sequencing in the species.
引文
Ackermann G, Tang YJ, Henderson JP, Rodloff AC, Silva Jr. J, Cohen SH. Electroporation of DNA sequences from the pathogenicity locus (PaLoc) of toxinogenic Clostridium difficile into a non-toxinogenic strain. Mol Cell Probes, 2001, 15: 301-306
Adachi K, Wakamatsu K, Ito S, Miyamoto N, Kokubo T. An oxygen transporter hemocyanin can act on the late pathway of melanin synthesis. Pigment Cell Research, 2005, 18: 214-219
Albertson RC, Streelman JT, Kocher TD. Directional selection has shaped the oral jaws of Lake Malawi cichlid fishes. Proc Natl Acad Sci USA, 2003, 100: 5252-5257
Ashida M, Brey PT. “Recent advances on the research of the insect prophenoloxidase cascade”. In: Molecular mechanisms of immune responses in insects, Brey PT, Hultmark D, eds. (London: Chapman and Hall), 1998, pp 135-173
Birren B, Green ED, Klapholz S, Myers RM, Riethman H, Roskams J. Bacterial Artificial Chromosomes. Birren B, et al. Cloning Systems (3), In Genome Analysis: A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1997, 242-295
Burke DT, Carle GF, Olson M. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science, 1987, 236: 806-812
Canilleri C, Lafleuriel J, Macadre C, Varoquaux F, Parmentier Y, Picard G, Caboche M, Bouchez D. A YAC contig map of Arabidopsis thaliana chromosome 3. Plant J, 1998, 14: 633-642
Chang YL, Tao Q, Scheuring C, Ding K, Meksem K, Zhang H-B. An integrated map of Arabidopsis thaliana for functional analysis of its genome sequence. Genetics, 2001, 159: 1231-1242
Chen S, Bao Z, Pan J, Hu J. Genetic diversity and specific markers in four scallop species, Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C. farreri. Acta oceanologica sinica (Acta oceanol sin), 2005, 24(4): 107-113
Choi S, Begum D, Koshinsky H, OW DW, Wing RA. A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination. Nucleic Acids Res, 2000, 28(7): E19
Chow S, Dougherty WJ, Sandifer PA. Meiotic chromosome complements and nuclear DNA contents of four species of shrimps of the genus Penaeus. Journal of Crustacean Biology, 1990, 10, pp29-36
Chumakov IM, Rigault P, Le Gall I, Bellanne-Chantelot C, Billault A, Guillou S, Soularue P, Guasconi G, Poullier E, Gros I et al. A YAC contig map of the human genome. Nature, 1995, 377 (6547 Suppl.): 175-297
Chung CT, Miller RH. A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res, 1988, 16 (8): 3580
Cohen SN, Chang ACY and Hsu L. Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of E. coli by R-factor DNA. Proc Natl Acad Sci USA, 1972, 69: 2110-2114
Cohen SN, Chang ACY, Boyer HW Helling RB. Construction of Biologically Functional Bacterial Plasmids In Vitro. Proc Natl Acad Sci USA, 1973, 70 (11): 3240-3244
Collins J, Hohn B. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. Proc Natl Acad Sci USA, 1978, 75 (9): 4242-4246
Coulson A, Sulston J, Brenner S, Karn J. Toward a physical map of the genome of the nematode Caenorhabditis elegans. Proc Natl Acad Sci, 1986, 83: 7821-7825
Cunningham C, Hikima J, Jenny MJ, Chapman RW, Fang GC, Saski C, Lundqvist ML, Wing RA, Cupit PM, Gross PS, Warr GW, Tomkins JP. New Resources for Marine Genomics: Bacterial Artificial Chromosome Libraries for the Eastern and Pacific Oysters (Crassostrea virginica and C. gigas). Mar Biotechnol, 2006, 8: 521-533
De Tomaso AW, Weissman IL. Construction and characterization of large-insert genomic libraries (BAC and Fosmid) from the ascidian Botryllus schlosseri and initial physical mapping of a histocompatibility locus. Mar Biotechnol, 2003, 5: 103-115
DeBruin K A, Krassowska W. Modeling electroporation in a single cell. I. Effects of field strength and rest potential. Biophys J, 1999, 77: 1213-1223
Ditta G, Stanfield S, Corbin D, Helinski D. Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium melilot. Proc Natl Acad Sci USA, 1980, 77: 7347-7351
Feng J, Vick BA, Lee MK, Zhang H-B, Jan CC. Construction of BAC and BIBAC libraries from sunflower and identification of linkage group-specific clones by overgo hybridization. Theor Appl Genet, 2006, 113: 23-32
Fiedler S, Wirth R. Transformation of bacteria with plasmid DNA by electroporation. Anal Biochem, 1988, 170 (1): 38-44
Frengen E, Weichenhan D, Zhao B, Osoegawa K, van Geel M, de Jong PJ. A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites. Genomics, 1999, 58(3): 250-253
Frijters ACJ, Zhang Z, van Damme M, Wang G, Ronald PC, Michelmore RW. Construction of a bacterial artificial chromosome library containing large EcoR I and Hind III genomic fragments of lettuce. Theor Appl Genet, 1997, 94: 390-399
Fu H, Dooner H K. A gene-enriched BAC library for cloning large allele-specific fragments from maize: isolation of a 240-kb contig of the bronze region. Genome Research, 2000, 10: 866-873
Gahr S A, Palti Y, Rexroad CE III. Genomic Characterization of a novel pair of Id genes in the rainbow trout (Oncorhynchus mykiss). Animal Genetics, 2004, 35: 317-320
G?tz P. “Mechanisms of encapsulation in dipteran hosts”. In: Immune mechanisms in invertebrate vectors, Lackie AM, eds. (Oxford: Oxford University Press), 1986, pp 1e20
Guo X, Ford SE, Zhang F. Molluscan aquaculture in China. Journal of Shellfish Research, 1999, 18: 19-31
Hamilton C M. A binary-BAC system for plant transformation with high-molecular- weight DNA. Gene, 1997, 200: 107-116
Hanahan D, Jersee J, Bloom FR. Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol, 1991, 204: 63-113
Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol, 1983, 166: 557-580
He L, Du C, Li Y, Scheuring C, Zhang H-B. “Large-insert bacterial clone libraries and their applications”. In: Aquaculture Genome Technologies, Liu Z, eds. (Ames: Blackwell Publishing), 2007, pp215-244
Hoffmann JA, Kafatos FC, Janeway CA, Ezekowitz RA. Phylogenetic perspectives in innate immunity. Science, 1999, 284 (5418): 1313-1318.
Hubert S, Hedgecook D. Linkage maps of microsatellite DNA markers for the Pacific oyster Crassosrtea gigas. Genetics, 2004, 168: 351-362
Inoue H, Nojima H, Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene, 1990, 96: 23-28
Ioannou P A, Amemiya C T, Garnes J, Kroisel PM, Shizuya H, Chen C, Batzer MA, de Jong PJ. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nature Genet, 1994, 6: 84-89
Ioannou P A, de Jong P J. Construction of bacterial artificial chromosome libraries using the modified P1 (PAC) system. Dracopoli N C, Haines J N, Korf B R, et al. Current Protocols in Human Genetics (5). New York: John Wiley & Sons, 1996, 5, 15
Jing X, Zhan WB. Characterisation of monoclonal antibodies to haemocyte types of scallop (Chlamys farreri). Fish Shellfish Immunol, 2005, 19(1): 17-25
Jones J D G, Shlumukov L, Carland F, English J, Scofield SR, Bishop GJ, Harrison K. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Research, 1992, 1: 285-297
Karsi A, Waldbieser GC. Partial cloning of t-cell receptor alpha (TCRa) gene and assignment to TCRa and TCRb genes to the catfish linkage map. Animal Genetics, 2004, 35: 150-151
Katagiri T, Asakawa S, Minagawa S, Shimizu N, Hirono I Aoki T. Construction and characterization of BAC libraries for three fish species: rainbow trout, carp and tilapia. Animal Genetics, 2001, 32: 200-204
Katagiri T, Kidd C, Tomasino E, Davis JT, Wishon C, Stern JE, Carleton, K, Howe AE, Kocher TD. A BAC-based physical map of the Nile tilapia genome. BMC Genomics, 2005, 6: 89
Katagiri T, Kidd C, Tomasino E, Davis JT, Wishon C, Stern JE, Carleton KL, Howe AE, Kocher TD. BAC-based physical map of the Nile tilapia genome. BMC Genomics, 2005, 6: 89-94
Kaur GP, Reddy DE, Zimonjic DB, de Riel JK, Athwal RS. Functional identification of a BAC clone from 16q24 carrying a senescence gene SEN16 for breast cancer cells. Oncogene, 2005, 24(1): 47-54
Kim U J, Birren B, Slepak T, Mancino V, Boysen C, Kang HL Simon MI, Shizuya H. Construction and characterization of a human bacterial artificial chromosome library. Genomics, 1996, 34: 213-218
Kim U J, Shizuya H, de Jong P J, Birren B, Simon MI. Stable propagation of cosmid size human DNA insert in an F factor based vector. Nucleic Acids Research, 1992, 20(5): 1083-1085
Kurata N, Umehara Y, Tanoue H, Sasaki T. Physical mapping of the rice genome with YAC clones. Plant Mol Biol, 1997, 35(1-2): 101-113
Lee B Y, Lee W J, Streelman J T, Carleton KL, Howe AE, Hulata G, Slettan A, Stern JE, Terai Y, Kocher TD. A second-generation genetic linkage map of tilapia (Oreochromis spp.). Genetics, 2005, 170(1): 237-244
Li L, Xiang JH, Liu X, Zhang Y, Dong B, Zhang XJ. Construction of AFLP-based genetic linkage map for Zhikong scallop, Chlamys farreri Jones et Preston and mapping of sex-linked markers. Aquaculture, 2005, 245: 63-73
Li Y, Byme K, Miggiano E, Whan V, Moore S, Keys S, Crocos P, Preston N, Lehnert S. Genetic mapping of the kuruma prawn Penaeus japonicus using AFLP markers. Aquaculture, 2003, 219: 143-156
Liu B, You F, Dong B, Xiang J. The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri. Chinese Journal of Oceanology and Limnology, 2006, 24(3): 295-299
Liu X, Chang Y, Xiang JH, Li F, Liu X, Brovkina EP. Studies on hybridization effects of different geographic population of Chlamys farreri. Acta Oceanol Sin, 2003, 25: 255-263
Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D. Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning. Proc Natl Acad Sci USA, 1999, 96: 6535-6540
Liu Z, Karsi A, Li P, Gao D, Dunham R. An AFLP-based genetic linkage map of channel catfish (lctalurus punctantus) constructed by using an interspecific hybrid resource family. Genetics, 2003, 165: 687-694
Mandel M, Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol, 1970, 53 (1): 159-162
Margulies M, Egholm M, Altman WE, et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature, 2005, 437: 376-380
McPherson J D, Marra M, Hillier L, Waterston R H et al. A physical map of the human genome. Nature, 2001, 409 (6822): 934-941
Medzhitov R, Janeway CA. Innate immunity, impact on the adaptive immune response. Current Opinion in Immunology, 1997, 9: 4-9
Meehan D, Xu Z, Zuniga G, Alcivar-Warren A. High frequency and large number of polymorphic microsatellites in cultured shrimp, Penaeus (Litopenaeus) vannamei. Mar Biotechnol, 2003, 5: 311-330
Mozo T, Fischer S, Shizuya H, Altmann T. Construction and characterization of the IGF Arabidopsis BAC library. Mol Gen Genet, 1998, 258: 562-570
Murray N E, Murray K. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature, 1974, 251 (5475): 476-481
Nagai T, Kawabata S. A Link between Blood Coagulation and Prophenol Oxidase Activation in Arthropod Host Defense. J Biol Chem, 2000, 275: 29264-29267
Ng S S H, Artieri C G, Bosdet I E, Chiu R, Danzmann RG, Davidson WS, Ferguson MM, Fjell CD, Hoyheim B, Jones SJ, de Jong PJ, Koop BF, Krzywinski MI, Lubieniecki K, Marra MA, Mitchell LA, Mathewson C, Osoegawa K, Parisotto
SE, Phillips RB, Rise ML, von Schalburg KR, Schein JE, Shin H, Siddiqui A, Thorsen J, Wye N, Yang G, Zhu B. A physical map of the genome of Atlantic salmon, Salmo salar. Genomics, 2005, 86(4): 396-404
Nichols K M, Young W P, Danzmann R G, Robison BD, Rexroad C, Noakes M et al. A consolidated linkage map for rainbow trout (Oncorhynchus mykiss). Animal Genetics, 2003, 34: 102-115
Nishimura A, Morita M, Nishimura Y, Sugino Y. A rapid and highly efficient method for preparation of competent Escherichia coli cells. Nucleic Acids Res, 1990, 18 (20): 6169
Nusbaum C, Slonim D K, Harris K L, Birren BW, Steen RG, Stein LD, Miller J, Dietrich WF, Nahf R, Wang V et al. A YAC-based physical map of the mouse genome. Nature Genet, 1999, 229(4): 388-393
Osoegawa K, Woon P Y, Zhao B, Frengen E, Tateno M, Catanese J, de Jong PJ. An improvement approach for construction of bacterial artificial chromosome libraries. Genomics, 1998, 52: 1-8
Palti Y, Gahr SA, Hansen JD. Characterization of a new BAC library for rainbow trout: evidence for multi-locus duplication. Animal Genetics, 2004, 35(2): 130-133
Pan J, Bao Z, Zhao Y, Wan J, Li Z, Hu X. The genetic diversity of different geographic Zhikong scallop (Chlamys farreri) populations. High Technol Lett, 2002, 12: 78-82
Pan Y, Li Q, Yu R, Wang R, Bao Z. Induction of gynogenesis and effects of ultraviolet irradiation on ultrastructure of sperm of the Zhikong scallop Chlamys farreri. Fisheries Science, 2004, 70(3): 487-496
Pérez F, Erazo C, Zhinaula M, Calderón J, Volckaert F. A sex-specific linkage map of the white shrimp Penaeus (Litopenaeus) vannamei based on AFLP markers. Aquaculture, 2005, 242: 105-118
Phillps R. Physical and genetic mapping of the rainbow trout major histocompatibility regions: evidence for duplication of the class I region. Immunogentics, 2003, 55: 561-569
Qin R, Wei W, Jin W, He G, Ning S Yu S, Song Y. Physical location of rice Gm-6,Pi-5(t) genes in O. officinalis with BAC-FISH. Chinese Science Bulletin, 2001, 46(8): 659-661
Qui L, Song L, Xu W, Ni D, Yu Y. Molecular cloning and expression of a Toll receptor genehomologue from Zhikong Scallop, Chlamys farreri. Fish & Shellfish Immunology, 2007, 22: 451-466
Quiniou SM, Katagiri T, Miller NW, Wilson M, Wolters WR, Waldbieser GC. Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genet Sel Evol, 2003, 35(6): 673-683
Ren C, Lee MK, Yan B, Ding K, Cox B, Romanov MN, Price JA, Dodgson JB, Zhang H-B. A BAC-based physical map of the chicken genome. Genome Res, 2003, 13: 2754-2758
Ren C, Xu ZY, Sun S, Lee MK, Wu C, Zhang H-B. “Genomic libraries for physical mapping”. In: The handbook of plant genome mapping: genetic and physical mapping, Meksem K and Kahl G, eds. (Weinheim: Wiley-VCH Verlag GmbH), 2005, pp 173-213
Rittich B, Spanova A. Electrotransformation of bacteria by plasmid DNAs: statistical evaluation of a model quantitatively describing the relationship between the number of electrotransformants and DNA concentration. Bioelectroch Bioener, 1996, 40: 233-238
Saji S, Umehara Y, Antonio B A, Yamane H, Tanoue H, Baba T, Aoki H, Ishige N, Wu JZ, Koike K, Matsumoto T, Sasaki T. A physical map with yeast artificial chromosome (YAC) clones covering 63% of the 12 rice chromosomes. Genome, 2001, 44: 32-37
Sakamoto T, Danzmann R G, Gharbi K, et al. A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics, 2000, 155: 1331-1345
Sambrook J, Maniatis T, Fritsch EF. Molecular cloning: a laboratory manual. (Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989)
Sambrook J, Russell D. Molecular Cloning: A laboratory Manual, 3rd ed. Science Publisher, Beijing, 2002
Schmidt R, West J, Love K, Lenehan Z, Lister C, Thompson H, Bouchez D, Dean C. Physical map and organization of Arabidopsis thaliana chromosome 4. Science, 1995, 270: 480-483
Serror P, Sasaki T, Ehrlich SD, Maguin E. Electroporation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids. Applied and Environmental Microbiology, 2002, 68: 46-52
Shen Y, Xiang J, Wang B, Li F, Tong W. Discovery of immune related factors in Fenneropenaeus chinensis by annotation of ESTs. Progress in nature science, 2004, 14(1): 47-54
Sheng Y, Mancino V, Birren B. Transformation of Escherichia coli with large DNA molecules by electroporation. Nucleic Acids Research, 1995, 23(11): 1990-1996
Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in
Escherichia coli using an F-factor-based vector. Proc Natl Acad Sci USA, 1992, 89: 8794-8797
Smith M, Jessee J, Landers T et al. High efficiency bacterial electroporation: 1 × 1010 E. coli transformants/μg. Focus, 1990, 12: 38-40
Soderlund C, Humphray S, Dunham A, French L. Contigs built with fingerprints, markers, and FPC V4.7. Genome Research, 2000, 10(11): 1772-1787
Song L, Li J, Li H, Cui Z, Li C, Xu W, Chang Y. The genetic structure and genetic differentiation of the natural population and the hatchery stock of Chlamys farreri revealed by RAPD analysis. High Technol Lett, 2002a, 7: 83-86
Song L, Xu W, Li C, Li H, Guo X, Xiang J. “Potential genes involved in immune response identified by expressed sequence tag analysis from scallop Chlamys farreri”. In: 5th Symposium on Diseases in Asia Aquaculture, 2002b, pp 141
Song L, Zou H, Chang Y, Xu W, Wu L. The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri. Dev Comp Immunol, 2006, 30(3): 265-73
Stein N, Feuillet C, Wicker T, Schlagenhauf E, Keller B. Subgenome chromosome walking in wheat: a 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.). Proc Natl Acad Sci USA, 2000, 97(24): 13436-41
Sternberg N. Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. Proc Natl Acad Sci USA, 1990, 87: 103-107
Su J, Song L, Xu W, Wu L, Li H, Xiang J. cDNA cloning and mRNA expression of the lipopolysaccharide- and beta-1,3-glucan-binding protein gene from scallop Chlamys farreri. Aquaculture, 2004, 239 (1/4): 69-80
Taketo A. DNA transfection of Escherichia coli by electroporation. Biochim Biophys Acta, 1988, 949 (3): 318-324
Tao Q, Chang YL, Wang J, Chen H, Islam-Faridi MN, Scheuring C, Wang B, Stelly DM, Zhang HB. Bacterial artificial chromosome-based physical map of the rice genome constructed by restriction fingerprint analysis. Genetics, 2001, 158: 1711-1724
Tao Q, Zhang H-B. Cloning and stable maintenance of DNA fragments over 300 kb in Escherichia coli with conventional plasmid-based vectors. Nucleic Acids Research, 1998, 26: 4901-4909
Thorsen J, Zhu B, Frengen E, Osoegawa K, de Jong PJ, Koop BF, Davidson WS, H?yheim B. A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects. BMC Genomics, 2005, 6: 50
Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, Smith HO, Yandell M, Evans CA, Holt RA, Gocayne JD, Amanatides P, Ballew RM, Huson DH, Wortman JR, Zhang Q, Kodira CD, Zheng XQH, Chen L, Skupski M, Subramanian G, Thomas PD, Zhang JH, Miklos GLG, Nelson C, Broder S, Clark AG, Nadeau C, McKusick VA, Zinder N, Levine AJ, Roberts RJ, Simon M, Slayman C, Hunkapiller M, Bolanos R, Delcher A, Dew I, Fasulo D, Flanigan M, Florea L, Halpern A, Hannenhalli S, Kravitz S, Levy S, Mobarry C, Reinert K, Remington K, Abu-Threideh J, Beasley E, Biddick K, Bonazzi V, Brandon R, Cargill M, Chandramouliswaran I, Charlab R, Chaturvedi K, Deng ZM, Di Francesco V, Dunn P, Eilbeck K, Evangelista C, Gabrielian AE, Gan W, Ge WM, Gong FC, Gu ZP, Guan P, Heiman TJ, Higgins ME, Ji RR, Ke ZX, Ketchum KA, Lai ZW, Lei YD, Li ZY, Li JY, Liang Y, Lin XY, Lu F, Merkulov GV, Milshina N, Moore HM, Naik AK, Narayan VA, Neelam B, Nusskern D, Rusch DB, Salzberg S, Shao W, Shue BX, Sun JT, Wang ZY, Wang AH, Wang X, Wang J, Wei MH, Wides R, Xiao CL, Yan CH, Yao A, Ye J, Zhan M, Zhang WQ, Zhang HY, Zhao Q, Zheng LS, Zhong F, Zhong WY, Zhu SPC, Zhao SY, Gilbert D, Baumhueter S, Spier G, Carter C, Cravchik A, Woodage T, Ali F, An HJ, Awe A, Baldwin D, Baden H, Barnstead M, Barrow I, Beeson K, Busam D, Carver A, Center A, Cheng ML, Curry L, Danaher S, Davenport L, Desilets R, Dietz S, Dodson K, Doup L, Ferriera S, Garg N, Gluecksmann A, Hart B, Haynes J, Haynes C, Heiner C, Hladun S, Hostin D, Houck J, Howland T, Ibegwam C, Johnson J, Kalush F, Kline L, Koduru S, Love A, Mann F, May D, McCawley S, McIntosh T, McMullen I, Moy M, Moy L, Murphy B, Nelson K, Pfannkoch C, Pratts E, Puri V, Qureshi H, Reardon M, Rodriguez R, Rogers YH, Romblad D, Ruhfel B, Scott R, Sitter C, Smallwood M, Stewart E, Strong R, Suh E, Thomas R, Tint NN, Tse S, Vech C, Wang G, Wetter J, Williams S, Williams M, Windsor S, Winn-Deen E, Wolfe K, Zaveri J, Zaveri K, Abril JF, Guigo R, Campbell MJ, Sjolander KV, Karlak B, Kejariwal A, Mi HY, Lazareva B, Hatton T, Narechania A, Diemer K, Muruganujan A, Guo N, Sato S, Bafna V, Istrail S, Lippert R, Schwartz R, Walenz B, Yooseph S, Allen D, Basu A, Baxendale J, Blick L, Caminha M, Carnes-Stine J, Caulk P, Chiang YH, Coyne M, Dahlke C, Mays AD, Dombroski M, Donnelly M, Ely D, Esparham S, Fosler C, Gire H, Glanowski S, Glasser K, Glodek A, Gorokhov M, Graham K, Gropman B, Harris M, Heil J, Henderson S, Hoover J, Jennings D, Jordan C, Jordan J, Kasha J, Kagan L, Kraft C, Levitsky A, Lewis M, Liu XJ, Lopez J, Ma D, Majoros W, McDaniel J, Murphy S, Newman M, Nguyen T, Nguyen N, Nodell M, Pan S, Peck J, Peterson M, Rowe W, Sanders R, Scott J, Simpson M, Smith T, Sprague A, Stockwell T, Turner R, Venter E, Wang M, Wen MY, Wu D, Wu M, Xia A, Zandieh A, Zhu XH. The sequence of the human genome. Science, 2001, 291(5507): 1304-1351
Waldbieser GC, Bosworth BG, Nonneman DJ, Wolters WR. A microsatellite-based genetic linkage map of the channel catfish, lctalurus punctantus. Genetics, 2001, 158: 727-734
Waldbieser GC, Karsi A, Gahr SA, Rexroad CE. Rapid development of gene-tagged microsatellite markers from bac clones of channel catfish, ictalurus punctatus, and rainbow trout, oncorhynchus mykiss. Genetics in Aquaculture Symp. VII. 2003, Nov 9-15, p22.
Wallis JW, Martien J, Groenen M, Crooijmans R, Layman D, Graves T, Scheer D, Kremitzki C, Fedele M, Mudd N, Cardenas M, Higginbotham J, Carter J, McGrane R, Gaige T, Mead K, Walker J, Albracht D, Davito J, Yang S, Leong S, Chinwalla A, Sekhon M, Wylie K, Dodgson J, Romanov M, Cheng H, de Jong PJ, Osoegawa K, Nefedov M, Zhang H-B McPherson J, Krzywinski M, Schein J, Hillier L, Mardis E, Wilson R, Warren W. A physical map of the chicken genome. Nature, 2004, 432: 761-764
Wang B, Li F, Dong B, Zhang X, Zhang C, Xiang JH. Discovery of genes in response to whit spot syndrome virus (WSSV) infection in Fenneropenaeus chinensis through cDNA microarray. Marine biotechnology, 2006, 8, 491-500
Wang H, Song L, Li C, Zhao J, Zhang H, Ni D, Xu W. Cloning and characterization of a novel C-type lectin from Zhikong scallop Chlamys farreri. Mol Immunol, 2007, 44(5): 722-31
Wang J, He X, Dai M, Ruan J, Chen J, Zhang Y, Hu Y, Ye C, Li S, Cong L, Fang L, Liu B, Li S, Wang J, Burt D, Wong G, Yu J, Yang H, Wang J. ChickVD: A sequence variation database in the chicken genome. Nucleic Acids Res, 2005, 33: 438-441
Wang L, Song L, Chang Y, i Xu W, Ni D, Guo X. A preliminary geneticmap of Zhikong scallop (Chlamys farreri Jones et Preston 1904). Aquaculture Research, 2005, 36: 643-653
Wang L, Song L, Zhang H, Gao Q, Guo X. Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819). Aquaculture Research, 2007, 38 (4): 409-419.
Wang S, Bao Z, Pan J. AFLP linkage map of an intraspecific cross in C. farreri. Journal of Shellfish Research, 2004, 23: 491-499
Wang S, Xu P, Thorsen J, Zhu B, de Jong PJ, Waldbieser G, Kucukatas H, Liu Z. Characterization of a BAC Library from Channel Catfish Ictalurus punctatus: Indications of High Levels of Chromosomal Reshuffling Among Teleost Genomes. Mar Biotechnol, 2007: 701-711
Wang TT, Choi YJ, Lee BH. Transformation Systems of non-Saccharomyces Yeast. Crit Rev Biotechnol, 2001, 21: 177-218
Wang Y, Guo X. Chromosomal rearrangement in Pectinidae revealed by rRNA loci and implications in bivalve evolution. Biological Bulletin, 2004, 207: 247-256
Watanabe M, Kobayashi N, Fujiyama A, Okada N. Construction of a BAC library for Haplochromis chilotes, a cichlid fish from Lake Victoria. Genes Genet Syst, 2003, 78: 103-105
Wernersson R, Schierup MH, Jorgensen FG, Gorodkin J, Panitz F, Staerfeldt HH, Christensen OF, Mailund T, Hornshoj H, Klein A, et al. Pigs in sequence space: a 0.66X coverage pig genome survey based on shotgun sequencing. BMC Genomics, 2005, 6, 70
Wu C, Sun S, Padmavathi N, Santos FA, Springman R, Ding K, Meksem K, Lightfoot DA, Zhang H-B. A BAC and BIBAC-based physical map of the soybean genome. Genome Res, 2004, 14: 319-326
Wu CC, Nimmakayala P, Santos FA, Springman R, Scheuring C, Meksem K, Lightfoot DA, Zhang HB. Construction and characterization of a soybean bacterial artificial chromosome library and use of multiple complementary libraries for genome physical mapping. Theor Appl Genet, 2004, 109(5): 1041-1050
Xia Q, Zhou Z, Lu C. A Draft Sequence for the Genome of the Domesticated Silkworm (Bombyx mori). Science, 2004, 306(5703): 1937-1940
Xiang JH, Wang B, Liu B, Li L, Wang ZZ, Wang XG, Tong W, Li FH. Over 10,000 expressed sequence tags from Fenneropenaeus chinensis. In: Plant, Animal & Microbe Genomes X Conference, Town & Country Convention Center, San Diego, CA, 2002
Xu P, Wang S, Liu L, Peatman E, Somridhivej B, Thimmapuram J, Gong G, Liu Z. Channel catfish BAC-end sequences for marker development and assessment of syntenic conservation with other fish species. Anim Genet, 2006, 37(4): 321-326
Xu Z, Sun S, Covaleda L, et al. Genome physical mapping with large-insert bacterial clones by fingerprint analysis: methodologies, source clone genome coverage, and contig map quality. Genomics, 2004, 84(6): 941-951
Yang H, Zhang F, Guo X. Triploid and Tetraploid Zhikong Scallop, Chlamys farreri Jones et Preston, Produced by Inhibiting Polar Body I. Mar Biotechnol, 2000, 2(5): 466-475
Yu J, Hu S, Wang J, Wong GK, Li S, Liu B, Deng Y, Dai L, Zhou Y, Zhang X, Cao M, Liu J, Sun J, Tang J, Chen Y, Huang X, Lin W, Ye C, Tong W, Cong L, Geng J, Han Y, Li L, Li W, Hu G, Huang X, Li W, Li J, Liu Z, Li L, Liu J, Qi Q, Liu J, Li L, Li T, Wang X, Lu H, Wu T, Zhu M, Ni P, Han H, Dong W, Ren X, Feng X, Cui P, Li X, Wang H, Xu X, Zhai W, Xu Z, Zhang J, He S, Zhang J, Xu J, Zhang K, Zheng X, Dong J, Zeng W, Tao L, Ye J, Tan J, Ren X, Chen X, He J, Liu D, Tian W, Tian C, Xia H, Bao Q, Li G, Gao H, Cao T, Wang J, Zhao W, Li P, Chen W, Wang X, Zhang Y, Hu J, Wang J, Liu S, Yang J, Zhang G, Xiong Y, Li Z, Mao L, Zhou C, Zhu Z, Chen R, Hao B, Zheng W, Chen S, Guo W, Li G, Liu S, Tao M, Wang J, Zhu L, Yuan Y, Yang H. A draft sequence of the rice genome (Oryza sativa L. ssp. indica).Science, 2002, 296(5565): 79-92
Yu Z, Guo X. Genetic linkage map of the eastern oyster Crassostrea virginica Gmelin. Biol Bull, 2003, 204: 327-338
Zhan A, Bao Z, Yao B, Wang X, Hui M, Hu J. Polymorphic microsatellite markers in the Zhikong scallop Chlamys farreri. Molecular Ecology Notes, 2006, 6(1): 127-129
Zhang F, Yang H. Analysis of the causes of mass mortality of farming Chlamys farreri in summer in costal areas of Shandong, China. Mar Sci, 1999, 1: 44-47
Zhang H-B, Woo S, Wing RA. “BAC, YAC and Cosmid Library Construction”. In: Plant Gene Isolation: Principles and Practice, Foster G and Twell D, eds. (England: John Wiley & Sons, Ltd), 1996, pp 75-99
Zhang H-B, Wu C. BAC as tools for genome sequencing. Plant Physiol Biochem, 2001, 39: 1-15
Zhang H-B, Zhao X, Ding X, Paterson AH, Wing RA. Preparation of megabase-size DNA from plant nuclei. Plant J, 1995, 7: 175-184
Zhang H-B. Construction and manipulation of large-insert bacterial clone libraries-manual. Texas A&M University, Texas, 2000. (http://hbz7.tamu.edu) Zhang H-B. Manual for construction and manipulation of large-insert bacterial clone libraries. Texas, USA: Texas A&M University, 2000
Zhang L, Bao Z, Cheng J, Li H, Huang X, Wang S, Zhang C, Hu J. Fosmid library construction and initial analysis of end sequences in Zhikong Scallop (Chlamys farreri). Mar Biotechnol, 2007, 9: 606-612
Zhang L, Bao Z, Wang S, Huang X, Hu J. Chromosome rearrangements in Pectinidae (Bivalvia: Pteriomorphia) implied based on chromosomal localization of histone H3 gene in four scallops. Genetica, 2007, 130: 193-198
Zhang L, Yang C, Zhang Y, Li L, Zhang X, Zhang Q, Xiang J. A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates. Genetica, 2007, 131: 37-49
Zhang Y, Zhan S, Cao P, Liu N, Chen X, Wang Y, Wang C. The Polypeptide in Chlamys farreri Can Protect Human Dermal Fibroblasts from Ultraviolet B Damage. Chinese Journal of Oceanology and Limnology, 2005, 23(3): 357-362
Zhao J, Song L, Li C, Zou H, Ni D, Wang W, Xu W. Molecular cloning of an invertebrate goose-type lysozyme gene from Chlamys farreri, and lytic activity of the recombinant protein. Mol Immun, 2007, 44(6): 1198-1208
Zhu L, Song L, Zhao J, Xu W, Chang Y. Molecular cloning, characterization and expression of a serine protease with clip-domain homologue from scallop Chlamys farreri, Fish & Shellfish Immunology, 2007, 22: 556-566
陈凡国,张学勇.大片段DNA插入文库的研究进展. 生物技术通报,2002(2): 1-5
贺桂珍, 王秀华,王崇明, 黄剑宇,王秀华,宋微波. 栉孔扇贝和海湾扇贝病原
体感染与疾病发生关系探讨. 高技术通讯, 2003, 13(3): 75-79
洪国藩. 水稻基因组工程. 上海:上海科学技术出版社,1999
李红蕾,宋林生,王玲玲,胥炜,相建海. 栉孔扇贝EST中微卫星标记的筛选.高技术通讯,2003(12): 72-75
倪多娇. 栉孔扇贝抗氧化酶基因的克隆与表达分析. 中国科学院博士学位论文,2007
宋微波,王崇明,王秀华,王秀华,李筠. 栉孔扇贝大规模死亡的病原研究新进展. 海洋科学,2001, 25(12): 23-26
王兵, 李富花, 谢玉素, 相建海. 中国明对虾凝血栓蛋白基因全长 cDNA 的克隆及其表达特征. 高技术通讯, 2006, 16(9): 964-969
相建海,周岭华,刘瑞玉.长毛对虾,短沟对虾和日本对虾的染色体研究.海洋科学, 1991, 4: 72-73
张福绥, 何义朝, 亓玲欣, 孙鲁宁. 海湾扇贝引种复状的研究. 海洋与湖沼, 1997, 28: 146-152.
张福绥,何义朝,杨红生.海湾扇贝引种工程及其综合效应. 中国工程科学, 2000, 2(2): 30-35
张国范, 李霞, 薛真福. 我国养殖扇贝大规模死亡的原因分析及防治对策. 中国水产, 1999, 9: 34-39.
张留所. 凡纳滨对虾分子标记筛选、连锁图谱构建和QTL定位. 中国科学院博士学位论文,2006.
张维翥, 吴信忠, 李登峰, 孙敬锋, 张扬, 杨霞. 海湾扇贝养殖过程中的流行病学调查研究. 海洋学报, 2005, 27(5): 137-144
Adachi K, Wakamatsu K, Ito S, Miyamoto N, Kokubo T. An oxygen transporter hemocyanin can act on the late pathway of melanin synthesis. Pigment Cell Research, 2005, 18: 214-219
Albertson RC, Streelman JT, Kocher TD. Directional selection has shaped the oral jaws of Lake Malawi cichlid fishes. Proc Natl Acad Sci USA, 2003, 100: 5252-5257
Ashida M, Brey PT. “Recent advances on the research of the insect prophenoloxidase cascade”. In: Molecular mechanisms of immune responses in insects, Brey PT, Hultmark D, eds. (London: Chapman and Hall), 1998, pp 135-173
Birren B, Green ED, Klapholz S, Myers RM, Riethman H, Roskams J. Bacterial Artificial Chromosomes. Birren B, et al. Cloning Systems (3), In Genome Analysis: A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1997, 242-295
Burke DT, Carle GF, Olson M. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science, 1987, 236: 806-812
Canilleri C, Lafleuriel J, Macadre C, Varoquaux F, Parmentier Y, Picard G, Caboche M, Bouchez D. A YAC contig map of Arabidopsis thaliana chromosome 3. Plant J, 1998, 14: 633-642
Chang YL, Tao Q, Scheuring C, Ding K, Meksem K, Zhang H-B. An integrated map of Arabidopsis thaliana for functional analysis of its genome sequence. Genetics, 2001, 159: 1231-1242
Chen S, Bao Z, Pan J, Hu J. Genetic diversity and specific markers in four scallop species, Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C. farreri. Acta oceanologica sinica (Acta oceanol sin), 2005, 24(4): 107-113
Choi S, Begum D, Koshinsky H, OW DW, Wing RA. A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination. Nucleic Acids Res, 2000, 28(7): E19
Chow S, Dougherty WJ, Sandifer PA. Meiotic chromosome complements and nuclear DNA contents of four species of shrimps of the genus Penaeus. Journal of Crustacean Biology, 1990, 10, pp29-36
Chumakov IM, Rigault P, Le Gall I, Bellanne-Chantelot C, Billault A, Guillou S, Soularue P, Guasconi G, Poullier E, Gros I et al. A YAC contig map of the human genome. Nature, 1995, 377 (6547 Suppl.): 175-297
Chung CT, Miller RH. A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res, 1988, 16 (8): 3580
Cohen SN, Chang ACY and Hsu L. Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of E. coli by R-factor DNA. Proc Natl Acad Sci USA, 1972, 69: 2110-2114
Cohen SN, Chang ACY, Boyer HW Helling RB. Construction of Biologically Functional Bacterial Plasmids In Vitro. Proc Natl Acad Sci USA, 1973, 70 (11): 3240-3244
Collins J, Hohn B. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. Proc Natl Acad Sci USA, 1978, 75 (9): 4242-4246
Coulson A, Sulston J, Brenner S, Karn J. Toward a physical map of the genome of the nematode Caenorhabditis elegans. Proc Natl Acad Sci, 1986, 83: 7821-7825
Cunningham C, Hikima J, Jenny MJ, Chapman RW, Fang GC, Saski C, Lundqvist ML, Wing RA, Cupit PM, Gross PS, Warr GW, Tomkins JP. New Resources for Marine Genomics: Bacterial Artificial Chromosome Libraries for the Eastern and Pacific Oysters (Crassostrea virginica and C. gigas). Mar Biotechnol, 2006, 8: 521-533
De Tomaso AW, Weissman IL. Construction and characterization of large-insert genomic libraries (BAC and Fosmid) from the ascidian Botryllus schlosseri and initial physical mapping of a histocompatibility locus. Mar Biotechnol, 2003, 5: 103-115
DeBruin K A, Krassowska W. Modeling electroporation in a single cell. I. Effects of field strength and rest potential. Biophys J, 1999, 77: 1213-1223
Ditta G, Stanfield S, Corbin D, Helinski D. Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium melilot. Proc Natl Acad Sci USA, 1980, 77: 7347-7351
Feng J, Vick BA, Lee MK, Zhang H-B, Jan CC. Construction of BAC and BIBAC libraries from sunflower and identification of linkage group-specific clones by overgo hybridization. Theor Appl Genet, 2006, 113: 23-32
Fiedler S, Wirth R. Transformation of bacteria with plasmid DNA by electroporation. Anal Biochem, 1988, 170 (1): 38-44
Frengen E, Weichenhan D, Zhao B, Osoegawa K, van Geel M, de Jong PJ. A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites. Genomics, 1999, 58(3): 250-253
Frijters ACJ, Zhang Z, van Damme M, Wang G, Ronald PC, Michelmore RW. Construction of a bacterial artificial chromosome library containing large EcoR I and Hind III genomic fragments of lettuce. Theor Appl Genet, 1997, 94: 390-399
Fu H, Dooner H K. A gene-enriched BAC library for cloning large allele-specific fragments from maize: isolation of a 240-kb contig of the bronze region. Genome Research, 2000, 10: 866-873
Gahr S A, Palti Y, Rexroad CE III. Genomic Characterization of a novel pair of Id genes in the rainbow trout (Oncorhynchus mykiss). Animal Genetics, 2004, 35: 317-320
G?tz P. “Mechanisms of encapsulation in dipteran hosts”. In: Immune mechanisms in invertebrate vectors, Lackie AM, eds. (Oxford: Oxford University Press), 1986, pp 1e20
Guo X, Ford SE, Zhang F. Molluscan aquaculture in China. Journal of Shellfish Research, 1999, 18: 19-31
Hamilton C M. A binary-BAC system for plant transformation with high-molecular- weight DNA. Gene, 1997, 200: 107-116
Hanahan D, Jersee J, Bloom FR. Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol, 1991, 204: 63-113
Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol, 1983, 166: 557-580
He L, Du C, Li Y, Scheuring C, Zhang H-B. “Large-insert bacterial clone libraries and their applications”. In: Aquaculture Genome Technologies, Liu Z, eds. (Ames: Blackwell Publishing), 2007, pp215-244
Hoffmann JA, Kafatos FC, Janeway CA, Ezekowitz RA. Phylogenetic perspectives in innate immunity. Science, 1999, 284 (5418): 1313-1318.
Hubert S, Hedgecook D. Linkage maps of microsatellite DNA markers for the Pacific oyster Crassosrtea gigas. Genetics, 2004, 168: 351-362
Inoue H, Nojima H, Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene, 1990, 96: 23-28
Ioannou P A, Amemiya C T, Garnes J, Kroisel PM, Shizuya H, Chen C, Batzer MA, de Jong PJ. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nature Genet, 1994, 6: 84-89
Ioannou P A, de Jong P J. Construction of bacterial artificial chromosome libraries using the modified P1 (PAC) system. Dracopoli N C, Haines J N, Korf B R, et al. Current Protocols in Human Genetics (5). New York: John Wiley & Sons, 1996, 5, 15
Jing X, Zhan WB. Characterisation of monoclonal antibodies to haemocyte types of scallop (Chlamys farreri). Fish Shellfish Immunol, 2005, 19(1): 17-25
Jones J D G, Shlumukov L, Carland F, English J, Scofield SR, Bishop GJ, Harrison K. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Research, 1992, 1: 285-297
Karsi A, Waldbieser GC. Partial cloning of t-cell receptor alpha (TCRa) gene and assignment to TCRa and TCRb genes to the catfish linkage map. Animal Genetics, 2004, 35: 150-151
Katagiri T, Asakawa S, Minagawa S, Shimizu N, Hirono I Aoki T. Construction and characterization of BAC libraries for three fish species: rainbow trout, carp and tilapia. Animal Genetics, 2001, 32: 200-204
Katagiri T, Kidd C, Tomasino E, Davis JT, Wishon C, Stern JE, Carleton, K, Howe AE, Kocher TD. A BAC-based physical map of the Nile tilapia genome. BMC Genomics, 2005, 6: 89
Katagiri T, Kidd C, Tomasino E, Davis JT, Wishon C, Stern JE, Carleton KL, Howe AE, Kocher TD. BAC-based physical map of the Nile tilapia genome. BMC Genomics, 2005, 6: 89-94
Kaur GP, Reddy DE, Zimonjic DB, de Riel JK, Athwal RS. Functional identification of a BAC clone from 16q24 carrying a senescence gene SEN16 for breast cancer cells. Oncogene, 2005, 24(1): 47-54
Kim U J, Birren B, Slepak T, Mancino V, Boysen C, Kang HL Simon MI, Shizuya H. Construction and characterization of a human bacterial artificial chromosome library. Genomics, 1996, 34: 213-218
Kim U J, Shizuya H, de Jong P J, Birren B, Simon MI. Stable propagation of cosmid size human DNA insert in an F factor based vector. Nucleic Acids Research, 1992, 20(5): 1083-1085
Kurata N, Umehara Y, Tanoue H, Sasaki T. Physical mapping of the rice genome with YAC clones. Plant Mol Biol, 1997, 35(1-2): 101-113
Lee B Y, Lee W J, Streelman J T, Carleton KL, Howe AE, Hulata G, Slettan A, Stern JE, Terai Y, Kocher TD. A second-generation genetic linkage map of tilapia (Oreochromis spp.). Genetics, 2005, 170(1): 237-244
Li L, Xiang JH, Liu X, Zhang Y, Dong B, Zhang XJ. Construction of AFLP-based genetic linkage map for Zhikong scallop, Chlamys farreri Jones et Preston and mapping of sex-linked markers. Aquaculture, 2005, 245: 63-73
Li Y, Byme K, Miggiano E, Whan V, Moore S, Keys S, Crocos P, Preston N, Lehnert S. Genetic mapping of the kuruma prawn Penaeus japonicus using AFLP markers. Aquaculture, 2003, 219: 143-156
Liu B, You F, Dong B, Xiang J. The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri. Chinese Journal of Oceanology and Limnology, 2006, 24(3): 295-299
Liu X, Chang Y, Xiang JH, Li F, Liu X, Brovkina EP. Studies on hybridization effects of different geographic population of Chlamys farreri. Acta Oceanol Sin, 2003, 25: 255-263
Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D. Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning. Proc Natl Acad Sci USA, 1999, 96: 6535-6540
Liu Z, Karsi A, Li P, Gao D, Dunham R. An AFLP-based genetic linkage map of channel catfish (lctalurus punctantus) constructed by using an interspecific hybrid resource family. Genetics, 2003, 165: 687-694
Mandel M, Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol, 1970, 53 (1): 159-162
Margulies M, Egholm M, Altman WE, et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature, 2005, 437: 376-380
McPherson J D, Marra M, Hillier L, Waterston R H et al. A physical map of the human genome. Nature, 2001, 409 (6822): 934-941
Medzhitov R, Janeway CA. Innate immunity, impact on the adaptive immune response. Current Opinion in Immunology, 1997, 9: 4-9
Meehan D, Xu Z, Zuniga G, Alcivar-Warren A. High frequency and large number of polymorphic microsatellites in cultured shrimp, Penaeus (Litopenaeus) vannamei. Mar Biotechnol, 2003, 5: 311-330
Mozo T, Fischer S, Shizuya H, Altmann T. Construction and characterization of the IGF Arabidopsis BAC library. Mol Gen Genet, 1998, 258: 562-570
Murray N E, Murray K. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature, 1974, 251 (5475): 476-481
Nagai T, Kawabata S. A Link between Blood Coagulation and Prophenol Oxidase Activation in Arthropod Host Defense. J Biol Chem, 2000, 275: 29264-29267
Ng S S H, Artieri C G, Bosdet I E, Chiu R, Danzmann RG, Davidson WS, Ferguson MM, Fjell CD, Hoyheim B, Jones SJ, de Jong PJ, Koop BF, Krzywinski MI, Lubieniecki K, Marra MA, Mitchell LA, Mathewson C, Osoegawa K, Parisotto
SE, Phillips RB, Rise ML, von Schalburg KR, Schein JE, Shin H, Siddiqui A, Thorsen J, Wye N, Yang G, Zhu B. A physical map of the genome of Atlantic salmon, Salmo salar. Genomics, 2005, 86(4): 396-404
Nichols K M, Young W P, Danzmann R G, Robison BD, Rexroad C, Noakes M et al. A consolidated linkage map for rainbow trout (Oncorhynchus mykiss). Animal Genetics, 2003, 34: 102-115
Nishimura A, Morita M, Nishimura Y, Sugino Y. A rapid and highly efficient method for preparation of competent Escherichia coli cells. Nucleic Acids Res, 1990, 18 (20): 6169
Nusbaum C, Slonim D K, Harris K L, Birren BW, Steen RG, Stein LD, Miller J, Dietrich WF, Nahf R, Wang V et al. A YAC-based physical map of the mouse genome. Nature Genet, 1999, 229(4): 388-393
Osoegawa K, Woon P Y, Zhao B, Frengen E, Tateno M, Catanese J, de Jong PJ. An improvement approach for construction of bacterial artificial chromosome libraries. Genomics, 1998, 52: 1-8
Palti Y, Gahr SA, Hansen JD. Characterization of a new BAC library for rainbow trout: evidence for multi-locus duplication. Animal Genetics, 2004, 35(2): 130-133
Pan J, Bao Z, Zhao Y, Wan J, Li Z, Hu X. The genetic diversity of different geographic Zhikong scallop (Chlamys farreri) populations. High Technol Lett, 2002, 12: 78-82
Pan Y, Li Q, Yu R, Wang R, Bao Z. Induction of gynogenesis and effects of ultraviolet irradiation on ultrastructure of sperm of the Zhikong scallop Chlamys farreri. Fisheries Science, 2004, 70(3): 487-496
Pérez F, Erazo C, Zhinaula M, Calderón J, Volckaert F. A sex-specific linkage map of the white shrimp Penaeus (Litopenaeus) vannamei based on AFLP markers. Aquaculture, 2005, 242: 105-118
Phillps R. Physical and genetic mapping of the rainbow trout major histocompatibility regions: evidence for duplication of the class I region. Immunogentics, 2003, 55: 561-569
Qin R, Wei W, Jin W, He G, Ning S Yu S, Song Y. Physical location of rice Gm-6,Pi-5(t) genes in O. officinalis with BAC-FISH. Chinese Science Bulletin, 2001, 46(8): 659-661
Qui L, Song L, Xu W, Ni D, Yu Y. Molecular cloning and expression of a Toll receptor genehomologue from Zhikong Scallop, Chlamys farreri. Fish & Shellfish Immunology, 2007, 22: 451-466
Quiniou SM, Katagiri T, Miller NW, Wilson M, Wolters WR, Waldbieser GC. Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genet Sel Evol, 2003, 35(6): 673-683
Ren C, Lee MK, Yan B, Ding K, Cox B, Romanov MN, Price JA, Dodgson JB, Zhang H-B. A BAC-based physical map of the chicken genome. Genome Res, 2003, 13: 2754-2758
Ren C, Xu ZY, Sun S, Lee MK, Wu C, Zhang H-B. “Genomic libraries for physical mapping”. In: The handbook of plant genome mapping: genetic and physical mapping, Meksem K and Kahl G, eds. (Weinheim: Wiley-VCH Verlag GmbH), 2005, pp 173-213
Rittich B, Spanova A. Electrotransformation of bacteria by plasmid DNAs: statistical evaluation of a model quantitatively describing the relationship between the number of electrotransformants and DNA concentration. Bioelectroch Bioener, 1996, 40: 233-238
Saji S, Umehara Y, Antonio B A, Yamane H, Tanoue H, Baba T, Aoki H, Ishige N, Wu JZ, Koike K, Matsumoto T, Sasaki T. A physical map with yeast artificial chromosome (YAC) clones covering 63% of the 12 rice chromosomes. Genome, 2001, 44: 32-37
Sakamoto T, Danzmann R G, Gharbi K, et al. A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics, 2000, 155: 1331-1345
Sambrook J, Maniatis T, Fritsch EF. Molecular cloning: a laboratory manual. (Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989)
Sambrook J, Russell D. Molecular Cloning: A laboratory Manual, 3rd ed. Science Publisher, Beijing, 2002
Schmidt R, West J, Love K, Lenehan Z, Lister C, Thompson H, Bouchez D, Dean C. Physical map and organization of Arabidopsis thaliana chromosome 4. Science, 1995, 270: 480-483
Serror P, Sasaki T, Ehrlich SD, Maguin E. Electroporation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids. Applied and Environmental Microbiology, 2002, 68: 46-52
Shen Y, Xiang J, Wang B, Li F, Tong W. Discovery of immune related factors in Fenneropenaeus chinensis by annotation of ESTs. Progress in nature science, 2004, 14(1): 47-54
Sheng Y, Mancino V, Birren B. Transformation of Escherichia coli with large DNA molecules by electroporation. Nucleic Acids Research, 1995, 23(11): 1990-1996
Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in
Escherichia coli using an F-factor-based vector. Proc Natl Acad Sci USA, 1992, 89: 8794-8797
Smith M, Jessee J, Landers T et al. High efficiency bacterial electroporation: 1 × 1010 E. coli transformants/μg. Focus, 1990, 12: 38-40
Soderlund C, Humphray S, Dunham A, French L. Contigs built with fingerprints, markers, and FPC V4.7. Genome Research, 2000, 10(11): 1772-1787
Song L, Li J, Li H, Cui Z, Li C, Xu W, Chang Y. The genetic structure and genetic differentiation of the natural population and the hatchery stock of Chlamys farreri revealed by RAPD analysis. High Technol Lett, 2002a, 7: 83-86
Song L, Xu W, Li C, Li H, Guo X, Xiang J. “Potential genes involved in immune response identified by expressed sequence tag analysis from scallop Chlamys farreri”. In: 5th Symposium on Diseases in Asia Aquaculture, 2002b, pp 141
Song L, Zou H, Chang Y, Xu W, Wu L. The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri. Dev Comp Immunol, 2006, 30(3): 265-73
Stein N, Feuillet C, Wicker T, Schlagenhauf E, Keller B. Subgenome chromosome walking in wheat: a 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.). Proc Natl Acad Sci USA, 2000, 97(24): 13436-41
Sternberg N. Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. Proc Natl Acad Sci USA, 1990, 87: 103-107
Su J, Song L, Xu W, Wu L, Li H, Xiang J. cDNA cloning and mRNA expression of the lipopolysaccharide- and beta-1,3-glucan-binding protein gene from scallop Chlamys farreri. Aquaculture, 2004, 239 (1/4): 69-80
Taketo A. DNA transfection of Escherichia coli by electroporation. Biochim Biophys Acta, 1988, 949 (3): 318-324
Tao Q, Chang YL, Wang J, Chen H, Islam-Faridi MN, Scheuring C, Wang B, Stelly DM, Zhang HB. Bacterial artificial chromosome-based physical map of the rice genome constructed by restriction fingerprint analysis. Genetics, 2001, 158: 1711-1724
Tao Q, Zhang H-B. Cloning and stable maintenance of DNA fragments over 300 kb in Escherichia coli with conventional plasmid-based vectors. Nucleic Acids Research, 1998, 26: 4901-4909
Thorsen J, Zhu B, Frengen E, Osoegawa K, de Jong PJ, Koop BF, Davidson WS, H?yheim B. A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects. BMC Genomics, 2005, 6: 50
Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, Smith HO, Yandell M, Evans CA, Holt RA, Gocayne JD, Amanatides P, Ballew RM, Huson DH, Wortman JR, Zhang Q, Kodira CD, Zheng XQH, Chen L, Skupski M, Subramanian G, Thomas PD, Zhang JH, Miklos GLG, Nelson C, Broder S, Clark AG, Nadeau C, McKusick VA, Zinder N, Levine AJ, Roberts RJ, Simon M, Slayman C, Hunkapiller M, Bolanos R, Delcher A, Dew I, Fasulo D, Flanigan M, Florea L, Halpern A, Hannenhalli S, Kravitz S, Levy S, Mobarry C, Reinert K, Remington K, Abu-Threideh J, Beasley E, Biddick K, Bonazzi V, Brandon R, Cargill M, Chandramouliswaran I, Charlab R, Chaturvedi K, Deng ZM, Di Francesco V, Dunn P, Eilbeck K, Evangelista C, Gabrielian AE, Gan W, Ge WM, Gong FC, Gu ZP, Guan P, Heiman TJ, Higgins ME, Ji RR, Ke ZX, Ketchum KA, Lai ZW, Lei YD, Li ZY, Li JY, Liang Y, Lin XY, Lu F, Merkulov GV, Milshina N, Moore HM, Naik AK, Narayan VA, Neelam B, Nusskern D, Rusch DB, Salzberg S, Shao W, Shue BX, Sun JT, Wang ZY, Wang AH, Wang X, Wang J, Wei MH, Wides R, Xiao CL, Yan CH, Yao A, Ye J, Zhan M, Zhang WQ, Zhang HY, Zhao Q, Zheng LS, Zhong F, Zhong WY, Zhu SPC, Zhao SY, Gilbert D, Baumhueter S, Spier G, Carter C, Cravchik A, Woodage T, Ali F, An HJ, Awe A, Baldwin D, Baden H, Barnstead M, Barrow I, Beeson K, Busam D, Carver A, Center A, Cheng ML, Curry L, Danaher S, Davenport L, Desilets R, Dietz S, Dodson K, Doup L, Ferriera S, Garg N, Gluecksmann A, Hart B, Haynes J, Haynes C, Heiner C, Hladun S, Hostin D, Houck J, Howland T, Ibegwam C, Johnson J, Kalush F, Kline L, Koduru S, Love A, Mann F, May D, McCawley S, McIntosh T, McMullen I, Moy M, Moy L, Murphy B, Nelson K, Pfannkoch C, Pratts E, Puri V, Qureshi H, Reardon M, Rodriguez R, Rogers YH, Romblad D, Ruhfel B, Scott R, Sitter C, Smallwood M, Stewart E, Strong R, Suh E, Thomas R, Tint NN, Tse S, Vech C, Wang G, Wetter J, Williams S, Williams M, Windsor S, Winn-Deen E, Wolfe K, Zaveri J, Zaveri K, Abril JF, Guigo R, Campbell MJ, Sjolander KV, Karlak B, Kejariwal A, Mi HY, Lazareva B, Hatton T, Narechania A, Diemer K, Muruganujan A, Guo N, Sato S, Bafna V, Istrail S, Lippert R, Schwartz R, Walenz B, Yooseph S, Allen D, Basu A, Baxendale J, Blick L, Caminha M, Carnes-Stine J, Caulk P, Chiang YH, Coyne M, Dahlke C, Mays AD, Dombroski M, Donnelly M, Ely D, Esparham S, Fosler C, Gire H, Glanowski S, Glasser K, Glodek A, Gorokhov M, Graham K, Gropman B, Harris M, Heil J, Henderson S, Hoover J, Jennings D, Jordan C, Jordan J, Kasha J, Kagan L, Kraft C, Levitsky A, Lewis M, Liu XJ, Lopez J, Ma D, Majoros W, McDaniel J, Murphy S, Newman M, Nguyen T, Nguyen N, Nodell M, Pan S, Peck J, Peterson M, Rowe W, Sanders R, Scott J, Simpson M, Smith T, Sprague A, Stockwell T, Turner R, Venter E, Wang M, Wen MY, Wu D, Wu M, Xia A, Zandieh A, Zhu XH. The sequence of the human genome. Science, 2001, 291(5507): 1304-1351
Waldbieser GC, Bosworth BG, Nonneman DJ, Wolters WR. A microsatellite-based genetic linkage map of the channel catfish, lctalurus punctantus. Genetics, 2001, 158: 727-734
Waldbieser GC, Karsi A, Gahr SA, Rexroad CE. Rapid development of gene-tagged microsatellite markers from bac clones of channel catfish, ictalurus punctatus, and rainbow trout, oncorhynchus mykiss. Genetics in Aquaculture Symp. VII. 2003, Nov 9-15, p22.
Wallis JW, Martien J, Groenen M, Crooijmans R, Layman D, Graves T, Scheer D, Kremitzki C, Fedele M, Mudd N, Cardenas M, Higginbotham J, Carter J, McGrane R, Gaige T, Mead K, Walker J, Albracht D, Davito J, Yang S, Leong S, Chinwalla A, Sekhon M, Wylie K, Dodgson J, Romanov M, Cheng H, de Jong PJ, Osoegawa K, Nefedov M, Zhang H-B McPherson J, Krzywinski M, Schein J, Hillier L, Mardis E, Wilson R, Warren W. A physical map of the chicken genome. Nature, 2004, 432: 761-764
Wang B, Li F, Dong B, Zhang X, Zhang C, Xiang JH. Discovery of genes in response to whit spot syndrome virus (WSSV) infection in Fenneropenaeus chinensis through cDNA microarray. Marine biotechnology, 2006, 8, 491-500
Wang H, Song L, Li C, Zhao J, Zhang H, Ni D, Xu W. Cloning and characterization of a novel C-type lectin from Zhikong scallop Chlamys farreri. Mol Immunol, 2007, 44(5): 722-31
Wang J, He X, Dai M, Ruan J, Chen J, Zhang Y, Hu Y, Ye C, Li S, Cong L, Fang L, Liu B, Li S, Wang J, Burt D, Wong G, Yu J, Yang H, Wang J. ChickVD: A sequence variation database in the chicken genome. Nucleic Acids Res, 2005, 33: 438-441
Wang L, Song L, Chang Y, i Xu W, Ni D, Guo X. A preliminary geneticmap of Zhikong scallop (Chlamys farreri Jones et Preston 1904). Aquaculture Research, 2005, 36: 643-653
Wang L, Song L, Zhang H, Gao Q, Guo X. Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819). Aquaculture Research, 2007, 38 (4): 409-419.
Wang S, Bao Z, Pan J. AFLP linkage map of an intraspecific cross in C. farreri. Journal of Shellfish Research, 2004, 23: 491-499
Wang S, Xu P, Thorsen J, Zhu B, de Jong PJ, Waldbieser G, Kucukatas H, Liu Z. Characterization of a BAC Library from Channel Catfish Ictalurus punctatus: Indications of High Levels of Chromosomal Reshuffling Among Teleost Genomes. Mar Biotechnol, 2007: 701-711
Wang TT, Choi YJ, Lee BH. Transformation Systems of non-Saccharomyces Yeast. Crit Rev Biotechnol, 2001, 21: 177-218
Wang Y, Guo X. Chromosomal rearrangement in Pectinidae revealed by rRNA loci and implications in bivalve evolution. Biological Bulletin, 2004, 207: 247-256
Watanabe M, Kobayashi N, Fujiyama A, Okada N. Construction of a BAC library for Haplochromis chilotes, a cichlid fish from Lake Victoria. Genes Genet Syst, 2003, 78: 103-105
Wernersson R, Schierup MH, Jorgensen FG, Gorodkin J, Panitz F, Staerfeldt HH, Christensen OF, Mailund T, Hornshoj H, Klein A, et al. Pigs in sequence space: a 0.66X coverage pig genome survey based on shotgun sequencing. BMC Genomics, 2005, 6, 70
Wu C, Sun S, Padmavathi N, Santos FA, Springman R, Ding K, Meksem K, Lightfoot DA, Zhang H-B. A BAC and BIBAC-based physical map of the soybean genome. Genome Res, 2004, 14: 319-326
Wu CC, Nimmakayala P, Santos FA, Springman R, Scheuring C, Meksem K, Lightfoot DA, Zhang HB. Construction and characterization of a soybean bacterial artificial chromosome library and use of multiple complementary libraries for genome physical mapping. Theor Appl Genet, 2004, 109(5): 1041-1050
Xia Q, Zhou Z, Lu C. A Draft Sequence for the Genome of the Domesticated Silkworm (Bombyx mori). Science, 2004, 306(5703): 1937-1940
Xiang JH, Wang B, Liu B, Li L, Wang ZZ, Wang XG, Tong W, Li FH. Over 10,000 expressed sequence tags from Fenneropenaeus chinensis. In: Plant, Animal & Microbe Genomes X Conference, Town & Country Convention Center, San Diego, CA, 2002
Xu P, Wang S, Liu L, Peatman E, Somridhivej B, Thimmapuram J, Gong G, Liu Z. Channel catfish BAC-end sequences for marker development and assessment of syntenic conservation with other fish species. Anim Genet, 2006, 37(4): 321-326
Xu Z, Sun S, Covaleda L, et al. Genome physical mapping with large-insert bacterial clones by fingerprint analysis: methodologies, source clone genome coverage, and contig map quality. Genomics, 2004, 84(6): 941-951
Yang H, Zhang F, Guo X. Triploid and Tetraploid Zhikong Scallop, Chlamys farreri Jones et Preston, Produced by Inhibiting Polar Body I. Mar Biotechnol, 2000, 2(5): 466-475
Yu J, Hu S, Wang J, Wong GK, Li S, Liu B, Deng Y, Dai L, Zhou Y, Zhang X, Cao M, Liu J, Sun J, Tang J, Chen Y, Huang X, Lin W, Ye C, Tong W, Cong L, Geng J, Han Y, Li L, Li W, Hu G, Huang X, Li W, Li J, Liu Z, Li L, Liu J, Qi Q, Liu J, Li L, Li T, Wang X, Lu H, Wu T, Zhu M, Ni P, Han H, Dong W, Ren X, Feng X, Cui P, Li X, Wang H, Xu X, Zhai W, Xu Z, Zhang J, He S, Zhang J, Xu J, Zhang K, Zheng X, Dong J, Zeng W, Tao L, Ye J, Tan J, Ren X, Chen X, He J, Liu D, Tian W, Tian C, Xia H, Bao Q, Li G, Gao H, Cao T, Wang J, Zhao W, Li P, Chen W, Wang X, Zhang Y, Hu J, Wang J, Liu S, Yang J, Zhang G, Xiong Y, Li Z, Mao L, Zhou C, Zhu Z, Chen R, Hao B, Zheng W, Chen S, Guo W, Li G, Liu S, Tao M, Wang J, Zhu L, Yuan Y, Yang H. A draft sequence of the rice genome (Oryza sativa L. ssp. indica).Science, 2002, 296(5565): 79-92
Yu Z, Guo X. Genetic linkage map of the eastern oyster Crassostrea virginica Gmelin. Biol Bull, 2003, 204: 327-338
Zhan A, Bao Z, Yao B, Wang X, Hui M, Hu J. Polymorphic microsatellite markers in the Zhikong scallop Chlamys farreri. Molecular Ecology Notes, 2006, 6(1): 127-129
Zhang F, Yang H. Analysis of the causes of mass mortality of farming Chlamys farreri in summer in costal areas of Shandong, China. Mar Sci, 1999, 1: 44-47
Zhang H-B, Woo S, Wing RA. “BAC, YAC and Cosmid Library Construction”. In: Plant Gene Isolation: Principles and Practice, Foster G and Twell D, eds. (England: John Wiley & Sons, Ltd), 1996, pp 75-99
Zhang H-B, Wu C. BAC as tools for genome sequencing. Plant Physiol Biochem, 2001, 39: 1-15
Zhang H-B, Zhao X, Ding X, Paterson AH, Wing RA. Preparation of megabase-size DNA from plant nuclei. Plant J, 1995, 7: 175-184
Zhang H-B. Construction and manipulation of large-insert bacterial clone libraries-manual. Texas A&M University, Texas, 2000. (http://hbz7.tamu.edu) Zhang H-B. Manual for construction and manipulation of large-insert bacterial clone libraries. Texas, USA: Texas A&M University, 2000
Zhang L, Bao Z, Cheng J, Li H, Huang X, Wang S, Zhang C, Hu J. Fosmid library construction and initial analysis of end sequences in Zhikong Scallop (Chlamys farreri). Mar Biotechnol, 2007, 9: 606-612
Zhang L, Bao Z, Wang S, Huang X, Hu J. Chromosome rearrangements in Pectinidae (Bivalvia: Pteriomorphia) implied based on chromosomal localization of histone H3 gene in four scallops. Genetica, 2007, 130: 193-198
Zhang L, Yang C, Zhang Y, Li L, Zhang X, Zhang Q, Xiang J. A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates. Genetica, 2007, 131: 37-49
Zhang Y, Zhan S, Cao P, Liu N, Chen X, Wang Y, Wang C. The Polypeptide in Chlamys farreri Can Protect Human Dermal Fibroblasts from Ultraviolet B Damage. Chinese Journal of Oceanology and Limnology, 2005, 23(3): 357-362
Zhao J, Song L, Li C, Zou H, Ni D, Wang W, Xu W. Molecular cloning of an invertebrate goose-type lysozyme gene from Chlamys farreri, and lytic activity of the recombinant protein. Mol Immun, 2007, 44(6): 1198-1208
Zhu L, Song L, Zhao J, Xu W, Chang Y. Molecular cloning, characterization and expression of a serine protease with clip-domain homologue from scallop Chlamys farreri, Fish & Shellfish Immunology, 2007, 22: 556-566
陈凡国,张学勇.大片段DNA插入文库的研究进展. 生物技术通报,2002(2): 1-5
贺桂珍, 王秀华,王崇明, 黄剑宇,王秀华,宋微波. 栉孔扇贝和海湾扇贝病原
体感染与疾病发生关系探讨. 高技术通讯, 2003, 13(3): 75-79
洪国藩. 水稻基因组工程. 上海:上海科学技术出版社,1999
李红蕾,宋林生,王玲玲,胥炜,相建海. 栉孔扇贝EST中微卫星标记的筛选.高技术通讯,2003(12): 72-75
倪多娇. 栉孔扇贝抗氧化酶基因的克隆与表达分析. 中国科学院博士学位论文,2007
宋微波,王崇明,王秀华,王秀华,李筠. 栉孔扇贝大规模死亡的病原研究新进展. 海洋科学,2001, 25(12): 23-26
王兵, 李富花, 谢玉素, 相建海. 中国明对虾凝血栓蛋白基因全长 cDNA 的克隆及其表达特征. 高技术通讯, 2006, 16(9): 964-969
相建海,周岭华,刘瑞玉.长毛对虾,短沟对虾和日本对虾的染色体研究.海洋科学, 1991, 4: 72-73
张福绥, 何义朝, 亓玲欣, 孙鲁宁. 海湾扇贝引种复状的研究. 海洋与湖沼, 1997, 28: 146-152.
张福绥,何义朝,杨红生.海湾扇贝引种工程及其综合效应. 中国工程科学, 2000, 2(2): 30-35
张国范, 李霞, 薛真福. 我国养殖扇贝大规模死亡的原因分析及防治对策. 中国水产, 1999, 9: 34-39.
张留所. 凡纳滨对虾分子标记筛选、连锁图谱构建和QTL定位. 中国科学院博士学位论文,2006.
张维翥, 吴信忠, 李登峰, 孙敬锋, 张扬, 杨霞. 海湾扇贝养殖过程中的流行病学调查研究. 海洋学报, 2005, 27(5): 137-144