棉花DNA分子标记图谱的构建及重要性状基因的定位
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摘要
以陆地棉(Gossypium hirsutum L．)多标记基因系T582和T586为亲本，构建了一个由120个单株组成的用于分子标记图谱构建的F_2作图群体，在考察和分析亲本、F_1和F_2群体质量性状和数量性状的基础上，利用SSR和AFLP分析技术，首先对各群体进行DNA分子标记的多态性分析，然后对显示多念性的标记进行连锁分析、图谱构建和农艺性状基因的定位。实验结果如下：1、对T582和T586两亲本间多态性分析，共获得了30个SSR标记和69个AFLP标记；2、应用Mapmaker3.0统计软件对形态标记和分子标记数据进行连锁分析，得到了一个由24个SSR标记、41个AFLP标记和8个形态标记共73个标记的棉花遗传图谱，该图谱有18个连锁群组成；3、根据形态标记基因所在的染色体信息，确定了5个连锁群与染色体的对应关系；4、利用QTL1.0统计软件对12个数量性状进行QTL分析，得到了8个性状的11个QTL位点，并分别进行了定位。
本研究首次用两个重要的棉花多基因标记系T582和T586作为作图亲本，对13个形态标记与DNA分子标记进行了连锁分析，并首次标记了3个单基因控制的形态性状，有效地将经典遗传连锁图谱与DNA分子标记连锁图谱联系起来，这对于促进棉花遗传学研究的深入具有重要意义。
Two multiple-markers lines (T582 and T586) of upland cotton (Gossypium hirsutum L. ) were used as parents to produce a F2 population consisting of 120 individual plants, which were used to construct a molecular linkage map. On the basis of observing the qualitative and quantative traits of the map population, Two types of DNA molecular marker methods, simple sequence repeals (SSR) and amplified fragment length polymorphism (AFLP), were used to construct a linkage map of molecular markers and to map genes of agronomic characters. The main results are as follows:
1. 30 SSR markers and 69 AFLP markers were polymorphic between T582 and T586.
2. All the polymorphic molecular markers and morphological markers were used to construct linkage map with Mapmaker3.0. This linkage map was composed of 73 markers including 24 SSR markers, 41 AFLP markers and 8 morphological markers. These 73 markers were assigned to 18 linkage groups.
3.According to the information of morphological markers in chromosomes, 5 linkage groups were assigned to the chromosomes.
4. 12 quantitive characters were analyzed with QTL1. 0 and 11 QTLs of 8 quantitive characters were detected.
This study first tagged three genes (cl,, fg, Lc1) of mophorlogical traits with DNA molecular markers and set up a relationship between classical genetic map and DNA molecular linkage map. It will benefit the studies of genetics and breeding in cotton.
本研究首次用两个重要的棉花多基因标记系T582和T586作为作图亲本，对13个形态标记与DNA分子标记进行了连锁分析，并首次标记了3个单基因控制的形态性状，有效地将经典遗传连锁图谱与DNA分子标记连锁图谱联系起来，这对于促进棉花遗传学研究的深入具有重要意义。
Two multiple-markers lines (T582 and T586) of upland cotton (Gossypium hirsutum L. ) were used as parents to produce a F2 population consisting of 120 individual plants, which were used to construct a molecular linkage map. On the basis of observing the qualitative and quantative traits of the map population, Two types of DNA molecular marker methods, simple sequence repeals (SSR) and amplified fragment length polymorphism (AFLP), were used to construct a linkage map of molecular markers and to map genes of agronomic characters. The main results are as follows:
1. 30 SSR markers and 69 AFLP markers were polymorphic between T582 and T586.
2. All the polymorphic molecular markers and morphological markers were used to construct linkage map with Mapmaker3.0. This linkage map was composed of 73 markers including 24 SSR markers, 41 AFLP markers and 8 morphological markers. These 73 markers were assigned to 18 linkage groups.
3.According to the information of morphological markers in chromosomes, 5 linkage groups were assigned to the chromosomes.
4. 12 quantitive characters were analyzed with QTL1. 0 and 11 QTLs of 8 quantitive characters were detected.
This study first tagged three genes (cl,, fg, Lc1) of mophorlogical traits with DNA molecular markers and set up a relationship between classical genetic map and DNA molecular linkage map. It will benefit the studies of genetics and breeding in cotton.
引文
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