人参皂苷Re对心肌缺血再灌注损伤中炎症反应及PGI_2/TXA_2系统的影响
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摘要
机体内组织器官的正常代谢和功能维持,都有赖于良好的血液循环,各种原因造成的局部组织或器官缺血,常常使组织细胞发生缺血性损伤。及时恢复缺血心肌组织的血液供应,不仅可以纠正由于缺血所引起的心肌代谢障碍,也可以使已发生可逆性结构变化的心肌恢复正常状态。但通过动物实验和临床观察发现,在一定条件下恢复血液再灌注后,部分动物或患者反而出现功能代谢障碍及结构破坏加重的现象,因而将这种血液再灌注使缺血性损伤进一步加重的现象称为缺血再灌注损伤。
目前尚缺乏预防与治疗心肌缺血再灌注损伤的特效中药,研究表明,人参皂苷Re具有保护心肌缺血再灌注损伤心肌的作用。本实验通过制备大鼠在体心肌缺血再灌注损伤模型,观察人参皂苷Re对心肌缺血再灌注损伤大鼠血流动力学、炎症反应,PGI2 /TXA2系统,以及氧自由基变化的影响及作用,为寻找安全有效的防治心肌缺血再灌注损伤的中药成分提供了实验依据。
结果表明,人参皂苷Re可以改变缺血再灌注损伤心肌的血流动力学参数,对缺血再灌注损伤心肌的舒缩功能有明显的改善作用;人参皂苷Re能够降低炎性因子的表达,减轻心肌组织的炎症损伤;人参皂苷Re能够起到调节PGI2 /TXA2平衡、降低血栓形成机率的作用;人参皂苷Re能够抑制脂质过氧化反应,保护抗氧化酶的活性,减少并清除有害降解产物的含量,起到减轻心肌缺血再灌注损伤的作用。
The normal metabolism and function of the tissues and organs are dependent on good blood circulation,if the blood supply of hearts decreases ,myocardium will be injured because of absolute or relative ischemia.By the experiments, we know that blood stream resuming after brief ischemia may caused myocardium injuried worse because of myocardial ischemia reperfusion injury (MIRI).Because the cardiovascular disease incidence rate increase,it is necessary to search a effective and safe traditional Chinese medicine to prevent MIRI. In this study ,We have evaluated the influence of Ginsenoside Re on the parameter of blood stream dynamics of Myocardium Ischemia-Reperfusion Injury,inflammation,PGI2/ TXA2 System,and MDA、SOD of serum.
Method:The MIRI models of Wistar rats in vivo were induced by ischemia left anterior descending coronary artery(LAD) for 30min and reperfused for 12 hours. We detected the effect of Ginsenoside Re on blood plasma of blood stream dynamics ;the inflammatory cytokines TNF-αand IL-1βof serum with RIA; and detected the SOD and MDA of serum;Observed the PGI2/ TXA2 System by detected the content of 6-keto-PGF1αand TXB2.
Result: 1. Compare with the MIRI group, SBP,DBP,MAP,LVSP,±dp/dtmax and HR of much dose group all increased obviously.In little dose group, SBP,DBP,LVSP,MAP,- dp/dtmax and HR increased,others have no significant changes; 2.Ginsenoside Re can decrease the inflammatory cytokines of TNF-αand IL1-βsignificantly; 3.Compare with the MIRI group,the group with Ginsenoside Re can decrease the content of TXB2 and the ratio of TXB2/6-keto-PGF1α,raise the content of 6-keto-PGF1αat the same time;4. Ginsenoside Re can decrease the content of MDA,and raise the SOD activeness prominent.
Conclusions: 1. Ginsenoside Re can improve the parameter of blood stream dynamics of Myocardium Ischemia-Reperfusion Injury; 2. Ginsenoside Re can decrease inflammatory factors of TNF-αand IL1-β, reduce the injury of myocardial tissue; 3.Adjusts the PGI2 /TXA2 balance; 4. Reduce the ROS,and reduces the injured myocardium of ischemia-reperfusion.
目前尚缺乏预防与治疗心肌缺血再灌注损伤的特效中药,研究表明,人参皂苷Re具有保护心肌缺血再灌注损伤心肌的作用。本实验通过制备大鼠在体心肌缺血再灌注损伤模型,观察人参皂苷Re对心肌缺血再灌注损伤大鼠血流动力学、炎症反应,PGI2 /TXA2系统,以及氧自由基变化的影响及作用,为寻找安全有效的防治心肌缺血再灌注损伤的中药成分提供了实验依据。
结果表明,人参皂苷Re可以改变缺血再灌注损伤心肌的血流动力学参数,对缺血再灌注损伤心肌的舒缩功能有明显的改善作用;人参皂苷Re能够降低炎性因子的表达,减轻心肌组织的炎症损伤;人参皂苷Re能够起到调节PGI2 /TXA2平衡、降低血栓形成机率的作用;人参皂苷Re能够抑制脂质过氧化反应,保护抗氧化酶的活性,减少并清除有害降解产物的含量,起到减轻心肌缺血再灌注损伤的作用。
The normal metabolism and function of the tissues and organs are dependent on good blood circulation,if the blood supply of hearts decreases ,myocardium will be injured because of absolute or relative ischemia.By the experiments, we know that blood stream resuming after brief ischemia may caused myocardium injuried worse because of myocardial ischemia reperfusion injury (MIRI).Because the cardiovascular disease incidence rate increase,it is necessary to search a effective and safe traditional Chinese medicine to prevent MIRI. In this study ,We have evaluated the influence of Ginsenoside Re on the parameter of blood stream dynamics of Myocardium Ischemia-Reperfusion Injury,inflammation,PGI2/ TXA2 System,and MDA、SOD of serum.
Method:The MIRI models of Wistar rats in vivo were induced by ischemia left anterior descending coronary artery(LAD) for 30min and reperfused for 12 hours. We detected the effect of Ginsenoside Re on blood plasma of blood stream dynamics ;the inflammatory cytokines TNF-αand IL-1βof serum with RIA; and detected the SOD and MDA of serum;Observed the PGI2/ TXA2 System by detected the content of 6-keto-PGF1αand TXB2.
Result: 1. Compare with the MIRI group, SBP,DBP,MAP,LVSP,±dp/dtmax and HR of much dose group all increased obviously.In little dose group, SBP,DBP,LVSP,MAP,- dp/dtmax and HR increased,others have no significant changes; 2.Ginsenoside Re can decrease the inflammatory cytokines of TNF-αand IL1-βsignificantly; 3.Compare with the MIRI group,the group with Ginsenoside Re can decrease the content of TXB2 and the ratio of TXB2/6-keto-PGF1α,raise the content of 6-keto-PGF1αat the same time;4. Ginsenoside Re can decrease the content of MDA,and raise the SOD activeness prominent.
Conclusions: 1. Ginsenoside Re can improve the parameter of blood stream dynamics of Myocardium Ischemia-Reperfusion Injury; 2. Ginsenoside Re can decrease inflammatory factors of TNF-αand IL1-β, reduce the injury of myocardial tissue; 3.Adjusts the PGI2 /TXA2 balance; 4. Reduce the ROS,and reduces the injured myocardium of ischemia-reperfusion.
引文
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