超声胃镜评价食管癌术前临床分期的价值研究
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摘要
目的：分析超声内镜(EUS)术前临床分期(CTNM)与术后病理分期(PTNM)符合率,评估EUS评价食管癌术前分期的临床价值。方法：对临床诊断为食管癌的58例行EUS检查及TNM分期,与手术病理分期结果比较。由于2009年以前病例采用2003年TNM分期,未能符合2009年修改的国际抗癌联盟制定的食管癌TNM分期标准,故EUS食管癌的TNM分期采用2003年修改的国际抗癌联盟制定的食管癌TNM分期法。结果：食管癌术前EUS检查T、N分期的诊断准确率分别为T1期71.4%。T2期71.4%,T3期80.0%,T4期66.7%,T分期总准确率为81.5%；NO期80.8%,N1期63.6%,N分期总准确率为70.7%。结论：EUS对食管癌的诊断及T、N分期诊断准确率较高,对指导术前制定治疗方案、评估预后有重要意义。
Objective:To evaluate the guidance value of EUS in preoperative clinical staging for diagnosis and treatment of esophageal cancer. Methods:58 patients with esophageal cancer were examined preoperatively by EUS, and staged according to the new TNM staging system(2003). The EUS findings were compared with surgical pathologic findings. Results:The accuracy rates of T staging by EUS were71.4% for Tl,71.4 % for T2,80.0% for T3,66.7% for T4, and 81.5% for T; those of N staging were 80.8 % for NO,63.6% for N1, and 70.7% for N. Conclusion:The accuracy rates of EUS are higher for diagnosis in esophageal cancer and preoperative staging. There is great significance for choosing ideal therapy plan for esophageal cancer, and for estimating prognosis of esophageal cancer.
Objective:To evaluate the guidance value of EUS in preoperative clinical staging for diagnosis and treatment of esophageal cancer. Methods:58 patients with esophageal cancer were examined preoperatively by EUS, and staged according to the new TNM staging system(2003). The EUS findings were compared with surgical pathologic findings. Results:The accuracy rates of T staging by EUS were71.4% for Tl,71.4 % for T2,80.0% for T3,66.7% for T4, and 81.5% for T; those of N staging were 80.8 % for NO,63.6% for N1, and 70.7% for N. Conclusion:The accuracy rates of EUS are higher for diagnosis in esophageal cancer and preoperative staging. There is great significance for choosing ideal therapy plan for esophageal cancer, and for estimating prognosis of esophageal cancer.
引文
[1]Hasegawa N, Niwa Y, Arisawa T, et al. Preoperative staging of superficial esophageal carcinoma:comparison of an ultrasound probe and stadard endoscopic ultrasonography[J]. Gastrointest Endosc,1996,44(4):388-393.
[2]Nishimaki T,Tanaka O, Ando N, et al. Evaluation of the accuracy of preoperative staging in thoracic esophageal cancer[J]. Ann Throac Surg,1999,68(6):2059-2064.
[3]Heidemann J, Schilling MK, Schmassmann A, et al. Accuracy of endoscopic ultrasonography in preoperative staging of esophageal carcinoma[J]. Dig Surg, 2000,17(3):219-224.
[4]Faigel DO. EUS in patients with benign and malignant lymphadenopathy[J]. Gastrointest Endosc,2001,53(6):593-598.
[5]Tumur Tsendsuren, Sun MT, Xu HM, et al. Usefulness of endoscopic ultrasonography in preoperative TNM staging of gastric cancer. World J Gastroenterol,2006,12(1):43-47.
[6]Wen TF, Hoichi KatoO, Wen HC, et al. Comparison of surgical management of thoracic esophageal carcinoma between two referral centers in Japan and China[J]. Japanese Journal of Clinical Oncology,2001,31:203-208.
[7]Rahul A. Shimpi, MD, Josh George, MD, Paul Jowell, et al, Staging of esophageal cancer by EUS:staging accuracy revisited, Gastrointestinal endoscopy Volume 66, No.3:2007;475-482
[8]胡炜,傅剑华,戎铁华等.超声内镜检查在食管癌术前临床分期的应用价值.癌症.2005,24(11)；1358-1362
[9]Murata Y, Ohta M, Hayashi K, Ide H, Takasaki K. Preoperative evaluation of lymph node metastasis in esophageal cancers. Ann Thorac Cardiovasc Surg 2003;9:88-92.
[10]Rudolf Mennigen, Dirk Tuebergen, Cabriele Koehler, et al, Endoscopic Ultrasound with Conventional Probe and Miniprobe in Preoperative Staging of Esophageal Cancer, J Gastrointest Surg (2008) 12:256-262
[11]H. Q. Peng, M.D.,B. D. Greenwald, M.D. F. R. Tavora, M.D.,et al. Evaluation of Performance of EUS-FNA in Preoperative Lymph Node Staging of Cancers of Esophagus, Lung, and Pancreas. Diagnostic Cytopathology, Vol 36, No 5290-296
[12]Willis J, Cooper GS, Isenberg G, et al. Correlation of EUS measurement with pathologic assessment of neoadjuvant therapy response in esophageal carcinoma. Gastrointest Endosc,2002,55(6):655-661
[13]Lowe VJ, Booya F, Fletcher JG, et al. Comparison of positron emission tomography, computed tomography, and endoscopic ultrasound in the initial Staging of patients with esophageal cancer. MolImagingBiol,2005,7(4): 422-430
[14]程贵余,苏凯,张汝刚等.内镜超声检查术在食管癌术前分期的临床应用.中华消化内镜杂志.2004年10月第21卷15期；310-312
[15]Romagnuolo J, Scott J, Hawes RH, et al. Helical CT versus EUS with fine needle aspiration for celiac nodal assessment in patients with esophageal cancer[J]. Gastrointest Endosc,2002,55(6):648-665.
[16]Eloubeidi MA, Wallace MB, Reed CE, et al. The utility of EUS and EUS-guided fine needle aspiration in detecting celiac lymph node metastasis in patients with esophageal cancer:a single-center experience[J]. Gastrointest Endosc,2001,54(6): 714-719.
[17]戎铁华,林鹏,吴一龙.胸段食管癌淋巴结转移的临床研究(附200例分析).中华胸心血管外科杂志,1994,10(3)：242-244.
[18]Weaver SR, Blackshaw GR.Lewis WG, et al. Comparison of special interest computed tomography.endosonography and histopathologieal stage ofoesophageal cancer[J]. Clin Radiol,2004,59(6):499-504.
[19]Pfau PR, Perlman SB.Stanko P, et al. The role and clinical value of EUS in 8 multimodalily esophageal carcinoma staging program with CT and positron emission tamography[J], Gastrointest Endose,2007,65(3):377-384.
[20]Lerut T, Flamen P, Eetors N, et al. Histopathologie validation of lymph node sta ng with FDG-PET scan in cancer of the esophagus and gastroesophageal junction: a prospective study based on primary surgery with extensive lymphadenec-tomy. Ann Surg,2000.232(6):743-752.
[21]Massari M, Pavoni GM, Cioffi U, et al. The role of echoendoscopy in the staging of squamous-cell carcinoma of the esophagus [J]. Minerva Chir,1999,54(4); 205-212.
[1]吕平,刘芳,吕坤章,戚昭恩,内窥镜发展史.中华医史杂志,2002,32(1)
[2]周殿元.我国消化内镜诊治现状与展望、中国实用内科杂志,2005,3
[3]张澍田,王拥军.内镜诊断技术的回顾与展望,临床消化病杂志,2007,2
[4]李兆申.回望2007：治疗内镜的进展(上).中国医学论坛报,2008,(1090)
[5]李兆申.回望2007：治疗内镜的进展(下).中国医学论坛报,2008,(1091)
[6]李兆申.新技术新希望新挑战-内镜粘膜下剥离术治疗早期胃癌进展,中国医学论坛报.2008,(1090)
[7]李姝,杨玉龙,刘文天.微型探头内镜超声对上消化道粘膜下肿物的诊断价值.中国实用内科杂志.2005,9
[8]Vilmann P, Saftoiu A. Endoscopic ultrasound guided fine needle aspiration biopsy:Equipment and technique. J Gastroenterol Hepatol,2006,21(11): 1646-1655. Review. PMID:16984583.
[9]刘之超,李锐,全怡.内镜超声引导下细针抽吸活检术的护理.现代临床医学.2007,4.
[10]Poppers DM, Haber GB. Endoscopic mucosalresection of colonic lesions:Current applications and future prospects. Med Clin North Am,2008,92(3):687-705. X. Review. PMID:18387382
[11]Hunerbein M, Ulmer C, Handke J, et al. Endosonography of upper gastrointestinal tract cancer on demand using miniprobes or endoscopic ultrasound [J]. Surg Endosc,2003.17(4):615-619.
[12]王强、童强、张卫国等,窄带成像放大内镜联合超声微探头诊断早期食管癌及癌前病变的价值,临床消化病杂志2009年第21卷第4期；217-219
[13]Lerut T, Flamen P, Eetors N, et al. Histopathologie validation of lymph node sta ng with FDG-PET scan in cancer of the esophagus and gastroesophageal junction:a prospective study based on primary surgery with extensive
lymphadenectomy.Ann Surg,2000.232(6):743-752.
[14]Massari M, Pavoni GM, Cioffi U, et al. The role of echoendoscopy in the staging of squamous-cell carcinoma of the esophagus [J]. Minerva Chir,1999,54(4); 205-212.
[15]Rahul A. Shimpi, MD, Josh George, MD, Paul Jowell, et al, Staging of esophageal cancer by EUS:staging accuracy revisited, Gastrointestinal endoscopy Volume 66, No.3:2007;475-482
[16]胡炜,傅剑华,戎铁华等.超声内镜检查在食管癌术前临床分期的应用价值.癌症,2005,24(11)；1358-1362
[17]Murata Y, Ohta M, Hayashi K, Ide H, Takasaki K. Preoperative evaluation of lymph node metastasis in esophageal cancers. Ann Thorac Cardiovasc Surg 2003;9:88-92.
[18]Rudolf Mennigen, Dirk Tuebergen, Cabriele Koehler, et al, Endoscopic Ultrasound with Conventional Probe and Miniprobe in Preoperative Staging of Esophageal Cancer, J Gastrointest Surg (2008) 12:256-262
[19]H. Q. Peng, M.D.,B. D. Greenwald, M.D. F. R. Tavora, M.D.,et al. Evaluation of Performance of EUS-FNA in Preoperative Lymph Node Staging of Cancers of Esophagus, Lung, and Pancreas. Diagnostic Cytopathology, Vol 36, No 5290-296
[20]Willis J, Cooper GS, Isenberg G, et al. Correlation of EUS measurement with pathologic assessment of neoadjuvant therapy response in esophageal carcinoma. Gastrointest Endosc,2002,55(6):655-661
[21]Lowe VJ, Booya F, Fletcher JG, et al. Comparison of positron emission tomography, computed tomography, and endoscopic ultrasound in the initial Staging of patients with esophageal cancer. MolImagingBiol,2005,7(4): 422-430
[22]程贵余,苏凯,张汝刚等.内镜超声检查术在食管癌术前分期的临床应用,中华消化内镜杂志2004年10月第21卷15期；310-312
[23]Richards DG, Brown TH, Manson JM, et al. Endoscopic uhrasound in the staging of tumours of the oesophagus and gastrooesophageal junction. Ann R Coil Surg Engl,2000,82(5):311-317.
[24]Gavin C. Harewood, K Shiva Kumar, Assessment of clinical impact of endoscopic ultrasound on esophageal cancer, Journal of Gastroenterology and Hepatology (2004)19,433-439
[25]郁春景,万卫星.18F-氟脱氧葡萄糖PET、超声内镜及CT在食管癌术前分期中的应用.国际放射医学核医学杂志.2007年5月第31卷第3期144-148.
[26]Pfau PR, Perlman SB. Stanko P, et al. The role and clinical value of EUS in a multimodalily esophageal carcinoma staging program'with CT and positron emission tamography[J], Gastrointest Endose,2007,65(3):377-384.
[27]Romagnuolo J, Scott J,Hawes RH, et al. Helical CT versus EUS with fine needle aspiration for celiac nodal assessment in patients with esophageal cancer [J]. Gastrointest Endosc,2002,55(6):648-665.
[28]张杰.超声内镜与CT仿真内镜对上消化道隆起样病变的诊断价值[J].中华消化内镜杂志,2003,4(2)：110-112.
[29]陈敏章.总论.见李益农,陆星华.消化内镜学[M].北京：科学出版社,1995.8-9.
[30]Abad R, Varas MJ, Espinos JC, et al. Transendoscopic ultrasonography with miniprobes[J]. Rev Esp Enferm Dig,2001,93(4):259-260.
[31]Weaver SR, Blackshaw GR.Lewis WG, et al. Comparison of special interest computed tomography.endosonography and histopathologieal stage ofoesophageal cancer[J]. Clin Radiol,2004,59(6):499-504.
[32]Pfau PR, Perlman SB.Stanko P, et al. The role and clinical value of EUS in 8 multimodalily esophageal carcinoma staging program with CT and positron emission tamography[J], Gastrointest Endose,2007,65(3):377-384.
[1]Cubitt AB, HeimT, AdamsSR, etal. Understanding,improving andusing green fluorescent proteins[J].TrendsBiochemSci,1995,Nov,20 (11):448-551
[2]KatzMH,TakimotoS,SpivackD,etal. Animageable highly metastatic orthotopic red fluorescent protein model of pancreatic cancer[J].ClinExpMetastasis,2004,21 (1):7-12.
[3]Heim R, Prasher DC, Tsien RY, etal. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA, 1994,91:12501-12504.
[4]Cunha J, Corgits A L, Herndon J E, etal. Molecular staging of polymerase chain reaction estimation of lymph node micrometastatic tumor cell burden in stage 1 non-small cell lung cancer:preliminary results of Cancer and Leukemia Group B Trial 9761 [J].J Thorac Cardiovasc Surg,2002,123:484-491.
[5]Babak Rashidi,Meng Yang, Ping Jiang, etal. A highly metastatic Lewis lung carcinoma orthotopic green fluorescent protein model[J].J Clinical & Experimental Metastasis,2000,18:57-60.
[6]Cunha J, Corgits A L, Herndon J E, etal. Molecular staging of polymerase chain reaction estimation of lymph node micrometastatic tumor cell burden in stage 1 non-small cell lung cancer:preliminary results of Cancer and Leukemia Group B Trial 9761[J].J Thorac Cardiovasc Surg,2002,123:484-491.
[7]李成继等.影响肺癌外科治疗预后因素的研究[J].中国肺癌杂志,2000,3：464-467.
[8]Yang M,Baranov E,Jiang P,etal.Whole-body optical imaging of green fluorescent protein-expression tumors and metastases [J].Proc Natl Acad USA,2000,97(3): 1206-1211.
[9]陈芳琳,陈正堂等.绿色荧光蛋白基因标记的肺癌细胞株的建立[J].重庆医学,
2007,36(19)：1938-1940.
[10]陈芳琳,辇伟奇,谢启超等.GFP标记的肺癌细胞生长和转移的在体荧光成像[J].现在生物医学进展,2008,8(7)：1214-1216.
[11]Yang M, Baranov E, Wang JW, etal. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc Natl Acad Sci USA,2002,99:3824-3829.
[12]Chishima T, Miyagi Y, Wang X, etal. Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression[J]. CancerRes, 1997,57:2042-2047.
[13]Chishima T, Miyagi Y,Wang X, etal. Metastatic patterns of lung cancer visualized live and in process by green fluorescent protein expression[J]. ClinExpMetastasis, 1997,15:547-552.
[14]Katz MH,Takimoto S,Spivack D,etal.Animageable highlymetastatic orthotopic red fluorescent protein model of pancreatic cancer[J]. ClinExpMetastasis,2004,21 (1):7-12.
[15]Kimura H, Hayashi K, Yamauchi K, et al. Real-time imaging of single cancer-cell dynamics of lung metastasis[J]. Journal of Cellular Biochemistry,2009, Nov 12,online.
[16]Kimie Nakagawa, Yuko Sasaki, Shigeaki Kato, etal.22-Oxa-1a,25-dihydroxy-vitamin D3 inhibits metastasis and angiogenesis in lung cancer[J]. Carcinogenesis, 2005,26(6):1044-1054.
[17]才志刚,张绍明,吴胜勇等.巨噬细胞炎症蛋白1a修饰的小鼠肺癌疫苗的制备及体内抗肿瘤活性研究[J].上海医学,2007,30(4)：269-272.
[18]Klein D, Indraccolo S, Von Rombs K, et al. Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker. Gene Ther,1997,4:1256-1260.
[19]朱圣明,王艳萍,陈晓未等.人端粒酶催化亚单位启动子驱动的EGFP真核表达 载体的构建及在肺癌细胞的表达[J].医学研究生学报,2007,20(2)：123-126.
[20]魏玲,宋现让,王兴武等.endostatin基因重组腺病毒的构建及在人肺腺癌细胞的表达[J].中国现代医学杂志.2008,18(6)：710-713.
[21]Umeoka, T. et al. Visualization of intrathoracically disseminated solid tumors in mice with optical imaging by telomerase-specific amplification of transferred green fluorescent protein gene. Cancer Res,2004,64,6259-6265.
[22]Hiroyuki Kishimoto, et al. In vivo imaging of lymph node metastasis withtelomerase-specific replication-selective adenovirus. Nature Medicine,2006,10 (12),1213-1219.
[23]Kishimoto H, et al. In vivo internal tumor illumination by telomerase-dependent adenoviral GFP for precise surgical navigation. Proc Natl Acad Sci U S A, 2009,106(34):14514-7.
[24]Depierre A, Mileron B, Moro Sibilot D,et al. Preoperative chemotherapy fo lowed by surgery compared with primary surgery in respectable stage I (excepTINO), Ⅱ and Ⅲa non-small cell lung cancer[J] J Clin Oncol,2002,20(1):247-253.
[2]Nishimaki T,Tanaka O, Ando N, et al. Evaluation of the accuracy of preoperative staging in thoracic esophageal cancer[J]. Ann Throac Surg,1999,68(6):2059-2064.
[3]Heidemann J, Schilling MK, Schmassmann A, et al. Accuracy of endoscopic ultrasonography in preoperative staging of esophageal carcinoma[J]. Dig Surg, 2000,17(3):219-224.
[4]Faigel DO. EUS in patients with benign and malignant lymphadenopathy[J]. Gastrointest Endosc,2001,53(6):593-598.
[5]Tumur Tsendsuren, Sun MT, Xu HM, et al. Usefulness of endoscopic ultrasonography in preoperative TNM staging of gastric cancer. World J Gastroenterol,2006,12(1):43-47.
[6]Wen TF, Hoichi KatoO, Wen HC, et al. Comparison of surgical management of thoracic esophageal carcinoma between two referral centers in Japan and China[J]. Japanese Journal of Clinical Oncology,2001,31:203-208.
[7]Rahul A. Shimpi, MD, Josh George, MD, Paul Jowell, et al, Staging of esophageal cancer by EUS:staging accuracy revisited, Gastrointestinal endoscopy Volume 66, No.3:2007;475-482
[8]胡炜,傅剑华,戎铁华等.超声内镜检查在食管癌术前临床分期的应用价值.癌症.2005,24(11)；1358-1362
[9]Murata Y, Ohta M, Hayashi K, Ide H, Takasaki K. Preoperative evaluation of lymph node metastasis in esophageal cancers. Ann Thorac Cardiovasc Surg 2003;9:88-92.
[10]Rudolf Mennigen, Dirk Tuebergen, Cabriele Koehler, et al, Endoscopic Ultrasound with Conventional Probe and Miniprobe in Preoperative Staging of Esophageal Cancer, J Gastrointest Surg (2008) 12:256-262
[11]H. Q. Peng, M.D.,B. D. Greenwald, M.D. F. R. Tavora, M.D.,et al. Evaluation of Performance of EUS-FNA in Preoperative Lymph Node Staging of Cancers of Esophagus, Lung, and Pancreas. Diagnostic Cytopathology, Vol 36, No 5290-296
[12]Willis J, Cooper GS, Isenberg G, et al. Correlation of EUS measurement with pathologic assessment of neoadjuvant therapy response in esophageal carcinoma. Gastrointest Endosc,2002,55(6):655-661
[13]Lowe VJ, Booya F, Fletcher JG, et al. Comparison of positron emission tomography, computed tomography, and endoscopic ultrasound in the initial Staging of patients with esophageal cancer. MolImagingBiol,2005,7(4): 422-430
[14]程贵余,苏凯,张汝刚等.内镜超声检查术在食管癌术前分期的临床应用.中华消化内镜杂志.2004年10月第21卷15期；310-312
[15]Romagnuolo J, Scott J, Hawes RH, et al. Helical CT versus EUS with fine needle aspiration for celiac nodal assessment in patients with esophageal cancer[J]. Gastrointest Endosc,2002,55(6):648-665.
[16]Eloubeidi MA, Wallace MB, Reed CE, et al. The utility of EUS and EUS-guided fine needle aspiration in detecting celiac lymph node metastasis in patients with esophageal cancer:a single-center experience[J]. Gastrointest Endosc,2001,54(6): 714-719.
[17]戎铁华,林鹏,吴一龙.胸段食管癌淋巴结转移的临床研究(附200例分析).中华胸心血管外科杂志,1994,10(3)：242-244.
[18]Weaver SR, Blackshaw GR.Lewis WG, et al. Comparison of special interest computed tomography.endosonography and histopathologieal stage ofoesophageal cancer[J]. Clin Radiol,2004,59(6):499-504.
[19]Pfau PR, Perlman SB.Stanko P, et al. The role and clinical value of EUS in 8 multimodalily esophageal carcinoma staging program with CT and positron emission tamography[J], Gastrointest Endose,2007,65(3):377-384.
[20]Lerut T, Flamen P, Eetors N, et al. Histopathologie validation of lymph node sta ng with FDG-PET scan in cancer of the esophagus and gastroesophageal junction: a prospective study based on primary surgery with extensive lymphadenec-tomy. Ann Surg,2000.232(6):743-752.
[21]Massari M, Pavoni GM, Cioffi U, et al. The role of echoendoscopy in the staging of squamous-cell carcinoma of the esophagus [J]. Minerva Chir,1999,54(4); 205-212.
[1]吕平,刘芳,吕坤章,戚昭恩,内窥镜发展史.中华医史杂志,2002,32(1)
[2]周殿元.我国消化内镜诊治现状与展望、中国实用内科杂志,2005,3
[3]张澍田,王拥军.内镜诊断技术的回顾与展望,临床消化病杂志,2007,2
[4]李兆申.回望2007：治疗内镜的进展(上).中国医学论坛报,2008,(1090)
[5]李兆申.回望2007：治疗内镜的进展(下).中国医学论坛报,2008,(1091)
[6]李兆申.新技术新希望新挑战-内镜粘膜下剥离术治疗早期胃癌进展,中国医学论坛报.2008,(1090)
[7]李姝,杨玉龙,刘文天.微型探头内镜超声对上消化道粘膜下肿物的诊断价值.中国实用内科杂志.2005,9
[8]Vilmann P, Saftoiu A. Endoscopic ultrasound guided fine needle aspiration biopsy:Equipment and technique. J Gastroenterol Hepatol,2006,21(11): 1646-1655. Review. PMID:16984583.
[9]刘之超,李锐,全怡.内镜超声引导下细针抽吸活检术的护理.现代临床医学.2007,4.
[10]Poppers DM, Haber GB. Endoscopic mucosalresection of colonic lesions:Current applications and future prospects. Med Clin North Am,2008,92(3):687-705. X. Review. PMID:18387382
[11]Hunerbein M, Ulmer C, Handke J, et al. Endosonography of upper gastrointestinal tract cancer on demand using miniprobes or endoscopic ultrasound [J]. Surg Endosc,2003.17(4):615-619.
[12]王强、童强、张卫国等,窄带成像放大内镜联合超声微探头诊断早期食管癌及癌前病变的价值,临床消化病杂志2009年第21卷第4期；217-219
[13]Lerut T, Flamen P, Eetors N, et al. Histopathologie validation of lymph node sta ng with FDG-PET scan in cancer of the esophagus and gastroesophageal junction:a prospective study based on primary surgery with extensive
lymphadenectomy.Ann Surg,2000.232(6):743-752.
[14]Massari M, Pavoni GM, Cioffi U, et al. The role of echoendoscopy in the staging of squamous-cell carcinoma of the esophagus [J]. Minerva Chir,1999,54(4); 205-212.
[15]Rahul A. Shimpi, MD, Josh George, MD, Paul Jowell, et al, Staging of esophageal cancer by EUS:staging accuracy revisited, Gastrointestinal endoscopy Volume 66, No.3:2007;475-482
[16]胡炜,傅剑华,戎铁华等.超声内镜检查在食管癌术前临床分期的应用价值.癌症,2005,24(11)；1358-1362
[17]Murata Y, Ohta M, Hayashi K, Ide H, Takasaki K. Preoperative evaluation of lymph node metastasis in esophageal cancers. Ann Thorac Cardiovasc Surg 2003;9:88-92.
[18]Rudolf Mennigen, Dirk Tuebergen, Cabriele Koehler, et al, Endoscopic Ultrasound with Conventional Probe and Miniprobe in Preoperative Staging of Esophageal Cancer, J Gastrointest Surg (2008) 12:256-262
[19]H. Q. Peng, M.D.,B. D. Greenwald, M.D. F. R. Tavora, M.D.,et al. Evaluation of Performance of EUS-FNA in Preoperative Lymph Node Staging of Cancers of Esophagus, Lung, and Pancreas. Diagnostic Cytopathology, Vol 36, No 5290-296
[20]Willis J, Cooper GS, Isenberg G, et al. Correlation of EUS measurement with pathologic assessment of neoadjuvant therapy response in esophageal carcinoma. Gastrointest Endosc,2002,55(6):655-661
[21]Lowe VJ, Booya F, Fletcher JG, et al. Comparison of positron emission tomography, computed tomography, and endoscopic ultrasound in the initial Staging of patients with esophageal cancer. MolImagingBiol,2005,7(4): 422-430
[22]程贵余,苏凯,张汝刚等.内镜超声检查术在食管癌术前分期的临床应用,中华消化内镜杂志2004年10月第21卷15期；310-312
[23]Richards DG, Brown TH, Manson JM, et al. Endoscopic uhrasound in the staging of tumours of the oesophagus and gastrooesophageal junction. Ann R Coil Surg Engl,2000,82(5):311-317.
[24]Gavin C. Harewood, K Shiva Kumar, Assessment of clinical impact of endoscopic ultrasound on esophageal cancer, Journal of Gastroenterology and Hepatology (2004)19,433-439
[25]郁春景,万卫星.18F-氟脱氧葡萄糖PET、超声内镜及CT在食管癌术前分期中的应用.国际放射医学核医学杂志.2007年5月第31卷第3期144-148.
[26]Pfau PR, Perlman SB. Stanko P, et al. The role and clinical value of EUS in a multimodalily esophageal carcinoma staging program'with CT and positron emission tamography[J], Gastrointest Endose,2007,65(3):377-384.
[27]Romagnuolo J, Scott J,Hawes RH, et al. Helical CT versus EUS with fine needle aspiration for celiac nodal assessment in patients with esophageal cancer [J]. Gastrointest Endosc,2002,55(6):648-665.
[28]张杰.超声内镜与CT仿真内镜对上消化道隆起样病变的诊断价值[J].中华消化内镜杂志,2003,4(2)：110-112.
[29]陈敏章.总论.见李益农,陆星华.消化内镜学[M].北京：科学出版社,1995.8-9.
[30]Abad R, Varas MJ, Espinos JC, et al. Transendoscopic ultrasonography with miniprobes[J]. Rev Esp Enferm Dig,2001,93(4):259-260.
[31]Weaver SR, Blackshaw GR.Lewis WG, et al. Comparison of special interest computed tomography.endosonography and histopathologieal stage ofoesophageal cancer[J]. Clin Radiol,2004,59(6):499-504.
[32]Pfau PR, Perlman SB.Stanko P, et al. The role and clinical value of EUS in 8 multimodalily esophageal carcinoma staging program with CT and positron emission tamography[J], Gastrointest Endose,2007,65(3):377-384.
[1]Cubitt AB, HeimT, AdamsSR, etal. Understanding,improving andusing green fluorescent proteins[J].TrendsBiochemSci,1995,Nov,20 (11):448-551
[2]KatzMH,TakimotoS,SpivackD,etal. Animageable highly metastatic orthotopic red fluorescent protein model of pancreatic cancer[J].ClinExpMetastasis,2004,21 (1):7-12.
[3]Heim R, Prasher DC, Tsien RY, etal. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA, 1994,91:12501-12504.
[4]Cunha J, Corgits A L, Herndon J E, etal. Molecular staging of polymerase chain reaction estimation of lymph node micrometastatic tumor cell burden in stage 1 non-small cell lung cancer:preliminary results of Cancer and Leukemia Group B Trial 9761 [J].J Thorac Cardiovasc Surg,2002,123:484-491.
[5]Babak Rashidi,Meng Yang, Ping Jiang, etal. A highly metastatic Lewis lung carcinoma orthotopic green fluorescent protein model[J].J Clinical & Experimental Metastasis,2000,18:57-60.
[6]Cunha J, Corgits A L, Herndon J E, etal. Molecular staging of polymerase chain reaction estimation of lymph node micrometastatic tumor cell burden in stage 1 non-small cell lung cancer:preliminary results of Cancer and Leukemia Group B Trial 9761[J].J Thorac Cardiovasc Surg,2002,123:484-491.
[7]李成继等.影响肺癌外科治疗预后因素的研究[J].中国肺癌杂志,2000,3：464-467.
[8]Yang M,Baranov E,Jiang P,etal.Whole-body optical imaging of green fluorescent protein-expression tumors and metastases [J].Proc Natl Acad USA,2000,97(3): 1206-1211.
[9]陈芳琳,陈正堂等.绿色荧光蛋白基因标记的肺癌细胞株的建立[J].重庆医学,
2007,36(19)：1938-1940.
[10]陈芳琳,辇伟奇,谢启超等.GFP标记的肺癌细胞生长和转移的在体荧光成像[J].现在生物医学进展,2008,8(7)：1214-1216.
[11]Yang M, Baranov E, Wang JW, etal. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc Natl Acad Sci USA,2002,99:3824-3829.
[12]Chishima T, Miyagi Y, Wang X, etal. Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression[J]. CancerRes, 1997,57:2042-2047.
[13]Chishima T, Miyagi Y,Wang X, etal. Metastatic patterns of lung cancer visualized live and in process by green fluorescent protein expression[J]. ClinExpMetastasis, 1997,15:547-552.
[14]Katz MH,Takimoto S,Spivack D,etal.Animageable highlymetastatic orthotopic red fluorescent protein model of pancreatic cancer[J]. ClinExpMetastasis,2004,21 (1):7-12.
[15]Kimura H, Hayashi K, Yamauchi K, et al. Real-time imaging of single cancer-cell dynamics of lung metastasis[J]. Journal of Cellular Biochemistry,2009, Nov 12,online.
[16]Kimie Nakagawa, Yuko Sasaki, Shigeaki Kato, etal.22-Oxa-1a,25-dihydroxy-vitamin D3 inhibits metastasis and angiogenesis in lung cancer[J]. Carcinogenesis, 2005,26(6):1044-1054.
[17]才志刚,张绍明,吴胜勇等.巨噬细胞炎症蛋白1a修饰的小鼠肺癌疫苗的制备及体内抗肿瘤活性研究[J].上海医学,2007,30(4)：269-272.
[18]Klein D, Indraccolo S, Von Rombs K, et al. Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker. Gene Ther,1997,4:1256-1260.
[19]朱圣明,王艳萍,陈晓未等.人端粒酶催化亚单位启动子驱动的EGFP真核表达 载体的构建及在肺癌细胞的表达[J].医学研究生学报,2007,20(2)：123-126.
[20]魏玲,宋现让,王兴武等.endostatin基因重组腺病毒的构建及在人肺腺癌细胞的表达[J].中国现代医学杂志.2008,18(6)：710-713.
[21]Umeoka, T. et al. Visualization of intrathoracically disseminated solid tumors in mice with optical imaging by telomerase-specific amplification of transferred green fluorescent protein gene. Cancer Res,2004,64,6259-6265.
[22]Hiroyuki Kishimoto, et al. In vivo imaging of lymph node metastasis withtelomerase-specific replication-selective adenovirus. Nature Medicine,2006,10 (12),1213-1219.
[23]Kishimoto H, et al. In vivo internal tumor illumination by telomerase-dependent adenoviral GFP for precise surgical navigation. Proc Natl Acad Sci U S A, 2009,106(34):14514-7.
[24]Depierre A, Mileron B, Moro Sibilot D,et al. Preoperative chemotherapy fo lowed by surgery compared with primary surgery in respectable stage I (excepTINO), Ⅱ and Ⅲa non-small cell lung cancer[J] J Clin Oncol,2002,20(1):247-253.