采收期及1-MCP对粉红女士苹果低温贮藏特性的影响
|
摘要
本论文以‘粉红女士’苹果为试材,研究了不同采收期对果实低温贮藏特性的影响,以及0℃贮藏期间和随后14d的货架期间,0.5μL·L-1浓度1-MCP(1-甲基环丙烯)对果实呼吸速率、乙烯释放速率、贮藏品质和可溶性蛋白质变化的影响,探讨了1-MCP影响苹果衰老的生理生化机制。试验结果表明:
(1)采收成熟度影响低温贮藏期间果实品质,特别是贮藏期超过3个月的果实。采收成熟度越高的果实,经长期贮藏后硬度、可滴定酸含量和可溶性固型物损失越大。因此,长期贮藏的果实宜在10月中旬采收,短期贮藏的果实宜在10月末或11月初采收。
(2)用0.5μL·L-1的1-MCP在20℃下处理‘粉红女士’苹果24h ,显著降低0℃贮藏期间果实呼吸速率、乙烯释放速率和ACC氧化酶活性,延缓果实硬度和可滴定酸含量下降。虽然不同成熟度的果实呼吸高峰和乙烯释放速率到达平台期的时间不同,但1-MCP处理对成熟度不同的果实品质影响无明显差异。
(3)在0℃1 80d的贮藏过程中,对照果实出现了37.1、18.0、16.6kD三条特异蛋白条带,0.5μL·L-11-MCP处理显著抑制37.1、18.0、16.6kD新蛋白质的表达,推测这些蛋白质与苹果衰老有关并受乙烯调控。
The storage behavior of‘Pink Lady’apples picked at 3 mature stages and the effects of 0.5μL·L-11-methylcyclopropene (1-MCP) on respiration, ethylene production, ACO (ACC oxidase) activity, storage quality and soluble proteins in apples with different maturity were studied during 6-months cold storage. The results were as follows.
(1). Harvest maturity influenced storage quality of‘Pink Lady’apples stored at 0℃, especially when the fruits were stored over 3 months. The more mature the fruits were harvested, the more loss happened in flesh firmness, titratable acids and soluble solids after long-term storage. Therefore, it was recommended that the fruit expected for a long-term storage be harvested at the late middle of October and the fruit expected for a short-term storage be harvest by the end of October.
(2). 1- MCP treatment (0.5μL·L-1 at 20℃for 24 hour) significantly inhibited the respiration, ethylene (C2H4) production and ACO activity, and slowed down the decrease in the flesh firmness and titratable acids of‘Pink Lady’apples during storage at 0℃. Some differences in the respiratory and ethylene peaks of fruits picked at three harvest dates were found. However there was no difference in the effects of 1-MCP on storage quality of 3-harvested fruits.
(3). SDS-PAGE analysis of soluble proteins showed three new protein bands, 37.1, 18.0, 16.6kD, in untreated fruit during 6-months storage at 0℃. The appearance of these proteins were delayed in 1- MCP (0.5μL·L-1 )treated fruits. It is speculated that those new proteins are related to the apple senescence and their synthesis in apples is regulated by ethylene.
(1)采收成熟度影响低温贮藏期间果实品质,特别是贮藏期超过3个月的果实。采收成熟度越高的果实,经长期贮藏后硬度、可滴定酸含量和可溶性固型物损失越大。因此,长期贮藏的果实宜在10月中旬采收,短期贮藏的果实宜在10月末或11月初采收。
(2)用0.5μL·L-1的1-MCP在20℃下处理‘粉红女士’苹果24h ,显著降低0℃贮藏期间果实呼吸速率、乙烯释放速率和ACC氧化酶活性,延缓果实硬度和可滴定酸含量下降。虽然不同成熟度的果实呼吸高峰和乙烯释放速率到达平台期的时间不同,但1-MCP处理对成熟度不同的果实品质影响无明显差异。
(3)在0℃1 80d的贮藏过程中,对照果实出现了37.1、18.0、16.6kD三条特异蛋白条带,0.5μL·L-11-MCP处理显著抑制37.1、18.0、16.6kD新蛋白质的表达,推测这些蛋白质与苹果衰老有关并受乙烯调控。
The storage behavior of‘Pink Lady’apples picked at 3 mature stages and the effects of 0.5μL·L-11-methylcyclopropene (1-MCP) on respiration, ethylene production, ACO (ACC oxidase) activity, storage quality and soluble proteins in apples with different maturity were studied during 6-months cold storage. The results were as follows.
(1). Harvest maturity influenced storage quality of‘Pink Lady’apples stored at 0℃, especially when the fruits were stored over 3 months. The more mature the fruits were harvested, the more loss happened in flesh firmness, titratable acids and soluble solids after long-term storage. Therefore, it was recommended that the fruit expected for a long-term storage be harvested at the late middle of October and the fruit expected for a short-term storage be harvest by the end of October.
(2). 1- MCP treatment (0.5μL·L-1 at 20℃for 24 hour) significantly inhibited the respiration, ethylene (C2H4) production and ACO activity, and slowed down the decrease in the flesh firmness and titratable acids of‘Pink Lady’apples during storage at 0℃. Some differences in the respiratory and ethylene peaks of fruits picked at three harvest dates were found. However there was no difference in the effects of 1-MCP on storage quality of 3-harvested fruits.
(3). SDS-PAGE analysis of soluble proteins showed three new protein bands, 37.1, 18.0, 16.6kD, in untreated fruit during 6-months storage at 0℃. The appearance of these proteins were delayed in 1- MCP (0.5μL·L-1 )treated fruits. It is speculated that those new proteins are related to the apple senescence and their synthesis in apples is regulated by ethylene.
引文
[1] Baile J B. Growth, maturation and senescence in fruits [J]. Science,1964,146: 880~888
[2] 孙希生,王文辉,李志强等. 1-MCP 对苹果采后生理的影响[J]. 保鲜与加工,2002,2(4): 3~7.
[3] 韩冬芳,马书尚,王鹰等. 1-MCP 对新红星苹果乙烯代谢和贮藏品质的影响[J]. 园艺学报,2003, 30(1):11~14
[4] 陈 莉,屠 康 ,赵艺泽等. 采后 1- MCP 和热处理对红富士苹果生理变化和贮藏品质的响[J].果树学报,2005,23(1):59~64.
[5] McMurchie E J, McGlasson W B, Eaks I L. Treatment of fruit with propylene gives information about the biogenesis of ethylene [J]. Nature,1972,237: 235~236
[6] Jiang Y M , Joyce D C , Macnish A J. Extension of the shelf life of banana fruit by 1-methylcyclopropene in combination with polyethylene bags [J]. Postharvest Biology and Technology, 1999, 16: 187~193.
[7] 刘愚. 果实成熟过程中乙烯的生成和作用[J]. 大自然探索,1986,5(3): 127~132.
[8] 石建新,赵迎丽,赵 猛等 .红富士苹果采后生理研究.山西农业科学 1999 , 27(2) :61~64
[9] 李富军. 1-MCP 对几种果实衰老的效应及调控机制研究. [D] .泰安:山东农业大学. 2004
[10] 吴有梅,顾采琴,部根福等. ABA 和乙烯在草莓采后成熟衰老中的作用 植物生理学报 l992.18 :167~168
[11] 顾采琴,关军锋,李广敏等:草莓和番茄果实乙烯自我催化与 Ca2+一 CaM 的关系[J]. 园艺学报,2003,30(3):263~266
[12] 徐昌杰, 陈昆松, 张上隆. 乙烯生物合成及其控制研究进展[J]. 植物学通报,1998,15 (增刊): 54~61
[13] Bayer EM Jr . A potent inhibitor of ethylene action in plant [J]. Plant Physiol,1976,58:268~271
[14] Cameron AC, Reid MS. The use of silver anionic complex as a foliar apray to prevent flowers abscission of Zygocactus [J]. HortSci,1981,16:761~762
[15] Reid MS, Paul JL, Farhoomand MB et al. Pulse treatments with the silver thiosukfate complex extend the vase life of cut carnations[J]. Amer Soc Hort Sci,1980,105:25~27
[16] Serek M, Reid MS. Anti-ethylene teeatments for [otted floweing plants-relative efficacy of inhibitors of ethylene action and biosynthesis [J]. HortSci,1993,28:1180~1181
[17] Sisler EC, Blankenship SM. Diazocyclopentadiene (DACP), a light sensitive reagent for the ethylene receptor on plants[J]. Plant Growth Regulation. 1993a,12:125~132
[18] Sisler EC, Serek M. Compounds controlling ethylene receptor. [J]. Bot Bull of Acad Sinica ,1999, 40:1~7
[19] Sisler EC. Ethylene-binding compenets in plants. In: Mattoo AK,Suttle JC (end). The Plant Hormone Ethylene [J]. Boca Raton: CRC Press,1991,81~89
[20] Fan X.T, Blankenship S M, Mattheis J P. 1-Methycyclopropene inhibits apple ripening [J]. Journal of the American Society for Horticultural Science,1999,124(6):690~695.
[21] Jeong J, Huber D J, Sargent S A. Influence of 1-methylcyclopropene (1-MCP) on ripening and cell wall matrix polysaccharides of avocado (Persea Americana) fruit [J]. Postharvest Biology and Technology,2002,25(3):241~256.
[22] Watkins CB,Nock J F,Whitaker BD. Responses of early,mid and late season apple cultivars to postharvest application of 1-methylcyclopropene (1-MCP) under air and controlled atmosphere storage conditions[J]. Postharvest Biology and Technology,2000,19(1):17~32.
[23] Sisler E, Dupille E, Serek M. Effect of 1-methylcyclopropene and methylcyclopropene on ethylene binding and ethylene action on cut carnations [J]. Plant Grow Regulation,1996,18:79~80
[24] 饶景萍,任小林.园艺产品贮运学 陕西人民出版社 181~18
[25] 高华,鲁玉妙,袁景军. 粉红女士苹果冷藏与常温贮藏的乙烯代谢及果实品质变化[J].西北林学院学报,2005,20(1):46~48
[26] 吴有梅,华雪增,方建雄等. 贮藏温度和气体对苹果乙烯生物合成的影响[J]. 植物生理学报. 1991,17(2):169~176
[27] Lay-Yee M, Dellapenna D, Ross G S. Changes in mRNA and protein during ripening in apple fruit (Malus domestica Borkh. cv Golden Delicious) [J].Plant Physiology,1990,94:850~853
[28] 牟其芸,张华,孙风三.果蔬中多酚氧化酶特性研究[J].天津农业科,1997,42,12~16
[29] 王爱国,罗广华.植物的超氧自由基与羟胺的反应关系.植物生理学通讯.1990,6:55
[30] 王菊.库尔勒香梨采后衰老与褐变关系的研究[D]. 新疆农业大学硕士学位论文,2002
[31] 乜兰春,孙建设,辛蓓等.苹果果实酶促褐变底物及多酚氧化酶活性的研究[J]. 园艺学报,2004,31(4):502~504.
[32] 乜兰春.苹果果实酚类和挥发性物质含量特征及其与果实品质关系的研究[D]. 河北农业大学,优秀博士论文,2004.
[33] 石建新, 赵猛, 赵迎丽, 梁小娥, 祁寿椿. 气调贮藏对富士苹果采后生理及果肉褐变的影响[J]. 果树科学,1999,(01)
[34] Anon. Mature Fuji more at risk for internal browning [J].Good Fruit Grower,1994,45(5):27
[35] 齐灵.富士苹果贮藏病害特点及其原因分析[J]. 果树科学,1995,12(3):179~181.
[36] 蒋跃明.荔枝果实采后果皮褐变的研究[D]. 广州:中山大学,1999
[37] 林植芳,李双顺,张东林等.采后荔枝果实中氧化和过氧化作用的变化[J]. 植物学报,1988b, 30(4): 382~38
[38] 蒋跃明,陈绵达. 林植芳等.香蕉低温酶促褐变[[J]. 植物生理学报,1991, 17(2):157~163
[39] 霍君生.果蔬采后生理衰变机制探讨 [A]. 见:赵可夫.植物抗性生理研究[C].济南:山东科学技术出版社,1992,255~260
[40] 生吉萍,申琳,罗云波.果蔬成熟和衰老中的重要酶—脂氧合酶.果树科学.1999,16(1):72~77
[41] 张玉星.水杨酸在苹果、梨果实成熟衰老中的效应分析[D]. 山东农业大学,1998
[42] Autio WR, Bramlage WJ. Effects of AVG on maturation,ripening,and storage of apples.
[43] Bleecker AB, Kenye WH, Somerville SC, Kende H. Use of monoclonal antibodies in the purification and characterization of 1-aminocyclopropane-1-carboxylate synthase,anenzyme in ethylene biosynthesis. Proc. Natl. Acta. Sci. USA,1986,83:7755~7759
[44] 单积修 脂氧合酶对草莓、苹果采后成熟衰老作用机制研究[D]北京:北京农业大学园艺系,1995.
[45] 陈昆松,徐昌杰,楼健等. 脂氧合酶与猕猴桃果实后熟软化的关系.植物生理学报.1999,25(2):138~144
[46] Bartley I M.Exo-polyglacturociase of apple[J].Phytochemistry.1978,17:213~216
[47] Bartley I 48M Knee M.Thechemistry of textural changes in fruit during storage[J].Food Chem.,1982,9:47~58
[48] 粱玉珐,敖良德,王明鑫等.多聚半乳糖醛酸酶(Pc)在苹果成熟过程中的作用[J].植物学报.1982.24(2):143—146.
[49] 方建雄,华雪增,胡愚.贮藏温度和气体状况对苹果果胶多聚半乳糖醛酸酶变化的影响[J].植物生理学报,1991.17(1):99~10
[50] Serek M, Sisler EC,Dupille E. Comparison of cyclopropene,1-methylcyclopropene and 3,3-dimethylcyclopropene as ethylene antagonists in plants. Plant Grow Regul, 1996, 18:169~174
[51] li Z M, Armugam S, Lazan H. β-glycosidase and its significance in ripening mango fruit. Phytochem,1995,38:1109~1114
[52] Aazan H, Selamat M K, Ali Z M. β-galactosidase,polygalacturonase and pectinesterase in differential soften and cell wall modification during papaya fruit ripening. Plant Physiol,1995,95:106~11
[53] Andres P K,Li S. Partial purification and characterization of β-galactosidase from sweet cherry, anonclimacteric fruit. J Agric Food Chem,1994,42:2177~2182
[54] Serek M, Sisler EC, Reid MS. 1-Methylcyclopropene, a novel gaseous inhibitor of ethylene action, improves the life of fruits,cut flowers and potted plants [J]. Acta Hort,1995,394:337~345
[55] Vereridis P , Johan P. Complete recomery in intro of ethylene forming enzyme activity [J]. Phytochemistry,1991,30:725
[56] Ross G F,Wegrzyn T, MouRae E A,et al. Apple β-galactosidase activity against cell wall polysaccharides and characterization of a related cDNA clone[J]. Plant Physiol,1994,106:521~528
[57] Kremer M G Kellogy J,Wayoner W, et al. Reduced ethylene synthesis and ripening control in tomatoes expression sadenosylmethionine hydrolase. NATO ASI Ser,1997,34:307~319
[58] Downs C G,Almira E C. A beta-galactosidase cDNA homolog from broccoli. Plant Physiol,1995,108:1342~1343
[59] Carey A T,Hott K,Picard S,et al. Tomato exo-(1,4) β-D-galactosidase isolation change during ripening in normal and mutant tomato fruit, and characterization of a related cDNA clone. Plant Physiol,1995,108:1099~1107
[60] Raghothama K G,Lowton K A,Goldborough P B,et al. Characterization of an ethylene regulated flower senescence-related gene from carnation. Plant Mol Bilo,1991,17:61~71
[61] 陈昆松,李芳,张上隆.猕猴桃果实成熟进程中木葡聚糖内糖转移酶 mRNA 水平的变化.植物学报,1999,41(11):1231~1234
[62] Siddiqui S,Bangerth F. Effect of pre-harvest application of calcium on flesh firmness and cell-wall composition of apples-influnce of fruit size. J Hortic Sci,1995,70:263~269
[63] King G A, Davies M. Cloning of a harvest-inducedβ-galactosidase from tips of harvest asparagus spears. Plant Physiol,1995,108:419~420
[64] Downs C G, Almira E C. A beta-galactosidase cDNA homolog from broccoli. Plant Physiol,1995,108:1342~1343
[65] Sisler EC. Ethylene activity of some pi acceptor compounds. Tobacco Sci,1977,2
[66] Sisler EC,Serek M. Inhibitors of ethylene responses in plants at the receptor level: resent development. Physiol Plant,1997,100:577~82
[67] Sisler EC. Ethylene-binding compenets in plants. In: Mattoo AK,Suttle JC (end). The Plant Hormone Ethylene. Boca Raton: CRC Press,1991,81~89
[68] Sisler EC. Ethylene activity of some pi acceptor compounds [J]. Tobacco Sci,1977,21:43~45
[69] Serek M, Sisler EC, Reid MS. Novel gaseous inhibitor of ethylene binding prevents ethylene effects in potted flowering plant [J]. J Amer Soc HortSci,1994,119:1230~1233
[70] Jeong J, Huber D J, Sargent S A. The potential benefits of 1-MCP for regulating the ripening and extending the storage of avocados[J] .HortSci,1999,34(3):538
[71] Serek M, Sisler EC, Reid MS. Effect of 1-MCP on vase life and ethylene response of cut flowers. Plant Grow Regul,1995,16:93~97
[72] Serek M, Dupille E, Sisler EC. Effect of 1-methylcyclopropene and methylenecyclopropene on ethylene binding and ethylene action on cut flower. Plant Grow Regul,1996,18:79~86
[73] Jiang Y M, Joyce D C, Macnish A J. Response of banana fruit to treatment with 1-methylcyclopropene. Plant Grow Regul,1999,77~82
[74] Mathooko FM, Tsunashima Y, Owino WZO. Regulation of genes encoding ethylene biosynthetic e nzymes in peach fruit by carbon dioxide and l-methylcyclopropene [J]. Postharvest Biol Technol, 2001,14:265~281
[75] Lelievre J M,Latche A,Bouzyen M,et al. Ethylene and fruit ripening [J]. Plant Physiol.,1997, 101:727~739
[76] Nakatsuka A, Murachi S, Okunishi H, et al. Differential expression and internal feedback regulation of 1-amiocyclopane-1-carboxylate synthase, 1-amiocyclopane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening [J]. Plant Physiol , 1998. 118:1295-1305.
[77] Macnish AJ, Joyce DC, Hofman PJ, et al.. 1-methylcyclopropene treatment efficacy inpreventing ethylene perception in banana fruit and grevillea and waxflower flowers. AusJ. Expl Agric,2000a,40:471~481
[78] Mitcham E , Bower J H , Biasi W V . Effects of repeated application of low concentrations of 1-MCP on storage and ripening of ‘bartlett’ pears. See: ⅩⅩⅥth International Horticultural Congress (Abstracts). Toronto Ganada. 2002. 236
[79] Kim HO, Hewett EW, Lallu N. Softening and ethylene production of kiwifruit reduced with 1-methylcyclopropene. Acta Hort. 2001,553:167~170
[80] Ku VVV, Wills R B H,Ben-Yehoshua S. 1-Methylcyclopropene can differentially affect the postharvest life of strawberries exposed to ethylene. HortiSci,1999,34(1):119~120
[81] Blankship SM, Dole JM. 1-methylcyclopropene: a review. Postharvest Biol. Technol.2003,28:1~25
[82] Fan X, Mattheis JP . Development of apple superficial scald, soft scald, core flush, and greasiness is reduced by MCP. J. Agric. Food Chem. 1999a,47:3063~3068
[83] Jiang YM , Joyce DC , Macnish A J . Effect of abscisic acid on banana fruit ripening inrelation to the role of ethylene. J. Plant Growth Regul.,2000,19:106~111
[84] Sisler E , Dupille E ,Serek M . Effect of 1-methylcyclopropene and methylcyclopropane on ethylene binding and ethylene action on cut carnations. Plant Grow Regul,1996a,18:79~86
[85] Wills RBH,Ku VVV. Use of 1-MCP to extend the time of ripening of green tomatoes and postharvest life of ripening tomatoes. Postharvest Biol. Technol. 2002,26:85~90
[86] Reid M, Dodge L, Joyce D C. Environmental effects on the inhibition of ethylene action by 1-MCP. Biology and Biotechnoligy of the Plant Hormone Ethylene. NATO Advanced Workshop, June 9~13, Creat, Greece. 1996.57
[87] Halevy A H,Mayak S. Senescence and posthavest physiology of cut flowers, Part 2. Hort Rew, 1981, 3:59~143
[88] Jiang Y, Joyce D C, Macnish A J. Extension of the shelf life of banana fruit by 1-methylcyclopropene in combination with polyethylene bags [J]. Postharvest Biol Technol, 1999, 16(2):187~193
[89] Mir NA, Curell E, Khan N, Whitaker M, Beaudry RM. Harvest mature, storage temperature, and 1~MCP application frequency after firmness retention and chlorophyllfluorescence of “Redchief Delicious” apples [J] Am. Soc. Hort. Sci. 2001,126:618~624
[90] Fan X, Argenta L, Mattheis JP. Interactive effects of 1-MCP and temperature on ‘Elberta’ peach quality[J]. HortScience. 2002,37(7):134~138
[91] Dauny PT, Joyce DC. 1-MCP improves storability of ‘Queen Cox’ and ‘Bramley’ apple fruit[J]. HortScience. 2002,37(7): 1082~1085
[92] Jiang YM,Joyce DC,Macnish AJ. Response of banana fruit to treatment with 1-methycyclopropene[J]. Plant Growth Regulation. 1999b,28:77~82
[93] 王文辉,孙希生,李志强,王志华,张志云.1-MCP 对软肉梨采后生理的影响[J]. 园艺学进展.2002,5:783~790
[94] Dong L, Luric S, Zhou H. Effects of 1-methylcyclopropene on ripening of “Canino”apricots and “Royal Zee” plums[J]. Postharvest Biol. Technol. 2002,24: 135~145
[95] Porat R, Weiss B, Cohen L, Avinoam D, et al.. Effects of ethylene and1-methylcyclopropene on the postharvest qualities of ‘shamouti’ oranges[J]. PostharvestBiol and Technol,1999, 15:155~163
[96] 王文辉,王志华,李志强等. 1-MCP 对鲜枣采后生理及保鲜效果的影响[J]. 保鲜与加工,2003,3(1):21~23
[97] Hoffman PJ, Jobin-Dccor M, Macnish AJ, Joyce DC. Ripening and quality responses of avocado, custard apple, mango and papaya fruit to 1-methylcyclopropene. Aust J. Exp. Agric. 2001,41:567~572
[98] Watkins C B, Nock J F, Whitaker B D. Responses of early, mid and late season apple cultivars to postharvest application of 1-methylcyclopropene(1-MCP) under air and controlled atmosphere storage conditions [J]. Postharvest Biol Technol,2000,19(1):17~32
[99] Golding J B ,Shearer D ,Wyllie S G ,et al. Application of 1-MCP and propylene to identify ethylene-dependent ripening processes in mature banana fruit. Postharvest Bioland Technol,1998,14:87~98
[100] 吴振先,张廷亮,陈永明,季作梁,陈维信.1-甲基环丙烯处理对不同成熟阶段香蕉果实后熟的影响.华南农业大学学报.2001,22(4):15~18
[101] Nazir A Mir, Erin Curelle, Najma Khan, et al. Harvest maturity, storage temperature, and 1-MCP application frequency alter firmness retention and chlorophyll fluorescence of ‘Redchief Delicious’ apples. J Amer Hort Sci, 2001, 126(5):618~624
[102] 孙希生,王文辉,王志华等. 1-MCP 对“金冠”苹果采后生理效应的影响.园艺学进展[J].2002.5: 729~734
[103] Kremer M G, Kellogy J, Wayoner W, et al. Reduced ethylene synthesis and ripening control in tomatoes expression sadenosylmethionine hydrolase [J]. NATO ASI Ser,1997,34:307~319
[104] Wills RBH,Ku VVV. Use of 1-MCP to extend the time of ripening of green tomatoes and postharvest life of ripening tomatoes [J]. Postharvest Biol. Technol. 2002,26:85~90
[105] Fan X.T., J.P. Mattheis, and S. Blankenship. Development of apple superficial scald, soft scald,core flush, and greasiness is reduced by MCP[J]. Agri. Food Chem.,1999,47 (8):3063~3068.
[106] Fan X.T.,J.P. Mattheis. Impact of 1-methylcyclopropene and methyl jasmonate on apple Volatile production. J. Agri,Food Chem.,1999,47 (7):2847~2853.
[107] Fan X.T., L. Argenta and JMattheis. Inhibition of ethylene action by 1-methylcyclopropene prolongs storage life of apricots. Postharvest Biol. Technol.,2000,20 (2):135~142.
[108] Fan X.T., J.P. Mattheis. Methyl jasmonate promotes apple fruit degreening independently of ethylene action[J]. Hort.Sci. 1999,34(2):310~312
[109] Song J., M.S. Tian, D.R. Dilley, et al. Effect of 1-MCP on fruit ripening and volatile production [J]. Hort. Sci.,1997,32:536.
[110] Fan X, Blankenship SM , Mattheis JP. 1-methylcyclopropene inhibits apple ripening [J]. Amer. Soc. Sci., 1999,124(6):690~695
[111] 孙希生,王文辉,李志强等. 1-MCP 对新红星苹果保鲜效果的影响. 中国园艺学会第九届学术年会论文集[J]. 北京:中国科学技术出版社,2001,51~55
[112] 孙希生,王文辉,王志华等. 1-MCP 对苹果采后生理的影响[J]. 保鲜与加工,2002,4:3~7
[113] Gong Y,Fan X, Mattheis JP. Responses of “Bing”and “Rainier” Sweet cherries to ethylene and 1-methylcyclopropene J Am. Soc. Hort. Sci.2002,127:831~835.
[114] Fan X, Argenta L, Mattheis JP. Inhibiton of ethylene action by 1-methylcyclopropene prolongs storage life of apricots. Postharvest Biol. Technol 2000a,20:135~142
[115] Dong L, Luric S, Zhou H. Effects of 1-methylcyclopropene on ripeningof“Canino”apricotsand “Royal Zee” plums. Postharvest Biol. Technol. 2002,24: 135~145.
[116] Serek M,Reid MS. Ethylene and postharvest performance of potted kalanchoe.Postharvest Biol and Technol,2000,18:43~48.
[117] 唐燕,马书尚, 武春林. 1-MCP 对嘎拉苹果呼吸、乙烯产生及贮藏品质的影响. 果树学报, 2004, 21(1): 42~45.
[118] Mir N A, Curell E, Khan N, et al. Harvest maturity, storage temperature, and 1-MCP application frequency alter firmness retention and chlorophyll fluorescence of `Red chief delicious` apples [J]. J Amer Soc Hort Sci, 2001,126(5):618~624
[119] Mattheis J, Fan X, Argenta L. Impact of 1-MCP on climacteric fruit volatile productionand aroma. See: ⅩⅩⅥ th International Horticultural Congress (Abstracts).Toronto Ganada. 2002. 235
[120] 孙希生, 王文辉, 王志华等. 1-MCP 对“金冠”苹果采后生理效应的影响. 园艺学进展. 2002, 5: 729~734
[121] Fan X, Argenta L, Mattheis JP. Interactive effects of 1-MCP and temperature on ‘Elberta’peach quality. HortScience. 2002,37(7):134~138
[122] Fan X, Argenta L ,Mattheis JP . Impacts of ionizing radiation on volatile production by ripening gala apple fruit. J Agric.Food Chem.,2001,49:254~26
[123] Feng X,Apelbum A,Sisler EC,Goren R. Control of ethylene responses in avocado fruit with 1-methylcyclopropene. Postharvest Biol. Technol. 2000,20:143~150
[124] Mattheis J, Fan X, Argenta L. Responses of apple and pear fruit to 1-methylcyclopropene [R]. Yakima WA: Proceedings of 16th Annual Postharvest Conference, 2000
[125] Trivedi P, Matto A, Solomos T. Effects of MCP and 1.52 kpa oxygen on apple scald control and alpha-farnesene biosynthesis. See: ⅩⅩⅥth International Horticultural Congress (Abstracts). TorontoGanada. 2002. 239
[126] CrippsJEL,Richards L A,Mariata AM.‘PinkLady’apple.Hortscience,1993(10):1057 .
[127] 樊秀彩,张继澍. 1-甲基环丙烯对采后猕猴桃果实生理效应的影响[J]. 园艺学报,2001,28(5):399~402
[128] 袁景军,赵政阳,王雷存. 粉红女士苹果发展建议[J]. 西北园艺 2003 04: 5~6
[129] WANG Q M,GUO D P,KYIKYI W YUAN J..Effect of 1-MCP on Delaying the Senescence of Broccoli and Its Possible Physiological Mechanism[J].Acta Hortculturae Sinica,2004,(31):205~209.
[130] Renate M, Edwarad C M, Margrethe S. Stress induced ethylene production, ethylene binding, and the response to the ethylene action inhibitor 1-MCP in miniature roses [J]. Scientia Hoeticulturae, 2000, 83: 51~59.
[131] Nadeau J A,Zhang X S, Nair H, O’Neill S D. Temporal and spatial regulation of 1-aminocyclopropane-1-carboxylate oxidase in the pollination-induced senescence of orchid flowers [J]. Plant Physiology,1993,103:31—39
[132] 陈毓醛. 生物化学研究技术[M]. 北京: 中国农业出版社, 1993: 198~200
[133] 牛锐敏 ,饶景萍 ,韩新花等. 不同采收期对红富士苹果贮藏品质的影响[J]. 西北农业学报 2006 15(3):171~174
[134] 王赵改,马书尚,王瑞庆等.1-MCP 对粉红女士苹果采后生理的影响[J].西北农林科技大学学报,2005,33:(5)123~126
[135] E A Curry. Effect of harvest Date and Oxygen level on Storability of Late Season Apple Cultivars. Controlled Atmosphere Research Conference,1989,1:103~110
[136] Lau O L. Harvest Indices for British Columbla-grown Apples. Controled Atmosphere Research Conference,1989,1:115~120.
[137] 颜志梅,盛宝龙,蔺 经等 翠冠梨不同采收期对贮藏性的影响[J].江苏农业科学,2005,1: 100~101
[138] 孙芳娟,韩明玉,赵彩平等. 采收成熟度对油桃贮藏品质的影响[J].西北植物学报,2007,27:(1)183~185
[139] 王瑞庆, 马书尚, 武春林等. ‘嘎拉’苹果对不同浓度 1-MCP 处理的反应[J]. 西北植物学报, 2005,25(2):256~261
[140] Lay-Yee M, Dean D, Gavin S R. Changes in mRNA and protein during ripening in apple fruit (Malus domestia Borkh. cv Golden Delicious)[J]. Plant Physiology,1990,94:850—853.
[141] Eva Dominguez-Puigjaner, Miguel Vendrell, M Dolors Ludevid. Differential protein accumulation in banana fruit during ripening[J]. Plant Physiol,1992,98:157~162
[2] 孙希生,王文辉,李志强等. 1-MCP 对苹果采后生理的影响[J]. 保鲜与加工,2002,2(4): 3~7.
[3] 韩冬芳,马书尚,王鹰等. 1-MCP 对新红星苹果乙烯代谢和贮藏品质的影响[J]. 园艺学报,2003, 30(1):11~14
[4] 陈 莉,屠 康 ,赵艺泽等. 采后 1- MCP 和热处理对红富士苹果生理变化和贮藏品质的响[J].果树学报,2005,23(1):59~64.
[5] McMurchie E J, McGlasson W B, Eaks I L. Treatment of fruit with propylene gives information about the biogenesis of ethylene [J]. Nature,1972,237: 235~236
[6] Jiang Y M , Joyce D C , Macnish A J. Extension of the shelf life of banana fruit by 1-methylcyclopropene in combination with polyethylene bags [J]. Postharvest Biology and Technology, 1999, 16: 187~193.
[7] 刘愚. 果实成熟过程中乙烯的生成和作用[J]. 大自然探索,1986,5(3): 127~132.
[8] 石建新,赵迎丽,赵 猛等 .红富士苹果采后生理研究.山西农业科学 1999 , 27(2) :61~64
[9] 李富军. 1-MCP 对几种果实衰老的效应及调控机制研究. [D] .泰安:山东农业大学. 2004
[10] 吴有梅,顾采琴,部根福等. ABA 和乙烯在草莓采后成熟衰老中的作用 植物生理学报 l992.18 :167~168
[11] 顾采琴,关军锋,李广敏等:草莓和番茄果实乙烯自我催化与 Ca2+一 CaM 的关系[J]. 园艺学报,2003,30(3):263~266
[12] 徐昌杰, 陈昆松, 张上隆. 乙烯生物合成及其控制研究进展[J]. 植物学通报,1998,15 (增刊): 54~61
[13] Bayer EM Jr . A potent inhibitor of ethylene action in plant [J]. Plant Physiol,1976,58:268~271
[14] Cameron AC, Reid MS. The use of silver anionic complex as a foliar apray to prevent flowers abscission of Zygocactus [J]. HortSci,1981,16:761~762
[15] Reid MS, Paul JL, Farhoomand MB et al. Pulse treatments with the silver thiosukfate complex extend the vase life of cut carnations[J]. Amer Soc Hort Sci,1980,105:25~27
[16] Serek M, Reid MS. Anti-ethylene teeatments for [otted floweing plants-relative efficacy of inhibitors of ethylene action and biosynthesis [J]. HortSci,1993,28:1180~1181
[17] Sisler EC, Blankenship SM. Diazocyclopentadiene (DACP), a light sensitive reagent for the ethylene receptor on plants[J]. Plant Growth Regulation. 1993a,12:125~132
[18] Sisler EC, Serek M. Compounds controlling ethylene receptor. [J]. Bot Bull of Acad Sinica ,1999, 40:1~7
[19] Sisler EC. Ethylene-binding compenets in plants. In: Mattoo AK,Suttle JC (end). The Plant Hormone Ethylene [J]. Boca Raton: CRC Press,1991,81~89
[20] Fan X.T, Blankenship S M, Mattheis J P. 1-Methycyclopropene inhibits apple ripening [J]. Journal of the American Society for Horticultural Science,1999,124(6):690~695.
[21] Jeong J, Huber D J, Sargent S A. Influence of 1-methylcyclopropene (1-MCP) on ripening and cell wall matrix polysaccharides of avocado (Persea Americana) fruit [J]. Postharvest Biology and Technology,2002,25(3):241~256.
[22] Watkins CB,Nock J F,Whitaker BD. Responses of early,mid and late season apple cultivars to postharvest application of 1-methylcyclopropene (1-MCP) under air and controlled atmosphere storage conditions[J]. Postharvest Biology and Technology,2000,19(1):17~32.
[23] Sisler E, Dupille E, Serek M. Effect of 1-methylcyclopropene and methylcyclopropene on ethylene binding and ethylene action on cut carnations [J]. Plant Grow Regulation,1996,18:79~80
[24] 饶景萍,任小林.园艺产品贮运学 陕西人民出版社 181~18
[25] 高华,鲁玉妙,袁景军. 粉红女士苹果冷藏与常温贮藏的乙烯代谢及果实品质变化[J].西北林学院学报,2005,20(1):46~48
[26] 吴有梅,华雪增,方建雄等. 贮藏温度和气体对苹果乙烯生物合成的影响[J]. 植物生理学报. 1991,17(2):169~176
[27] Lay-Yee M, Dellapenna D, Ross G S. Changes in mRNA and protein during ripening in apple fruit (Malus domestica Borkh. cv Golden Delicious) [J].Plant Physiology,1990,94:850~853
[28] 牟其芸,张华,孙风三.果蔬中多酚氧化酶特性研究[J].天津农业科,1997,42,12~16
[29] 王爱国,罗广华.植物的超氧自由基与羟胺的反应关系.植物生理学通讯.1990,6:55
[30] 王菊.库尔勒香梨采后衰老与褐变关系的研究[D]. 新疆农业大学硕士学位论文,2002
[31] 乜兰春,孙建设,辛蓓等.苹果果实酶促褐变底物及多酚氧化酶活性的研究[J]. 园艺学报,2004,31(4):502~504.
[32] 乜兰春.苹果果实酚类和挥发性物质含量特征及其与果实品质关系的研究[D]. 河北农业大学,优秀博士论文,2004.
[33] 石建新, 赵猛, 赵迎丽, 梁小娥, 祁寿椿. 气调贮藏对富士苹果采后生理及果肉褐变的影响[J]. 果树科学,1999,(01)
[34] Anon. Mature Fuji more at risk for internal browning [J].Good Fruit Grower,1994,45(5):27
[35] 齐灵.富士苹果贮藏病害特点及其原因分析[J]. 果树科学,1995,12(3):179~181.
[36] 蒋跃明.荔枝果实采后果皮褐变的研究[D]. 广州:中山大学,1999
[37] 林植芳,李双顺,张东林等.采后荔枝果实中氧化和过氧化作用的变化[J]. 植物学报,1988b, 30(4): 382~38
[38] 蒋跃明,陈绵达. 林植芳等.香蕉低温酶促褐变[[J]. 植物生理学报,1991, 17(2):157~163
[39] 霍君生.果蔬采后生理衰变机制探讨 [A]. 见:赵可夫.植物抗性生理研究[C].济南:山东科学技术出版社,1992,255~260
[40] 生吉萍,申琳,罗云波.果蔬成熟和衰老中的重要酶—脂氧合酶.果树科学.1999,16(1):72~77
[41] 张玉星.水杨酸在苹果、梨果实成熟衰老中的效应分析[D]. 山东农业大学,1998
[42] Autio WR, Bramlage WJ. Effects of AVG on maturation,ripening,and storage of apples.
[43] Bleecker AB, Kenye WH, Somerville SC, Kende H. Use of monoclonal antibodies in the purification and characterization of 1-aminocyclopropane-1-carboxylate synthase,anenzyme in ethylene biosynthesis. Proc. Natl. Acta. Sci. USA,1986,83:7755~7759
[44] 单积修 脂氧合酶对草莓、苹果采后成熟衰老作用机制研究[D]北京:北京农业大学园艺系,1995.
[45] 陈昆松,徐昌杰,楼健等. 脂氧合酶与猕猴桃果实后熟软化的关系.植物生理学报.1999,25(2):138~144
[46] Bartley I M.Exo-polyglacturociase of apple[J].Phytochemistry.1978,17:213~216
[47] Bartley I 48M Knee M.Thechemistry of textural changes in fruit during storage[J].Food Chem.,1982,9:47~58
[48] 粱玉珐,敖良德,王明鑫等.多聚半乳糖醛酸酶(Pc)在苹果成熟过程中的作用[J].植物学报.1982.24(2):143—146.
[49] 方建雄,华雪增,胡愚.贮藏温度和气体状况对苹果果胶多聚半乳糖醛酸酶变化的影响[J].植物生理学报,1991.17(1):99~10
[50] Serek M, Sisler EC,Dupille E. Comparison of cyclopropene,1-methylcyclopropene and 3,3-dimethylcyclopropene as ethylene antagonists in plants. Plant Grow Regul, 1996, 18:169~174
[51] li Z M, Armugam S, Lazan H. β-glycosidase and its significance in ripening mango fruit. Phytochem,1995,38:1109~1114
[52] Aazan H, Selamat M K, Ali Z M. β-galactosidase,polygalacturonase and pectinesterase in differential soften and cell wall modification during papaya fruit ripening. Plant Physiol,1995,95:106~11
[53] Andres P K,Li S. Partial purification and characterization of β-galactosidase from sweet cherry, anonclimacteric fruit. J Agric Food Chem,1994,42:2177~2182
[54] Serek M, Sisler EC, Reid MS. 1-Methylcyclopropene, a novel gaseous inhibitor of ethylene action, improves the life of fruits,cut flowers and potted plants [J]. Acta Hort,1995,394:337~345
[55] Vereridis P , Johan P. Complete recomery in intro of ethylene forming enzyme activity [J]. Phytochemistry,1991,30:725
[56] Ross G F,Wegrzyn T, MouRae E A,et al. Apple β-galactosidase activity against cell wall polysaccharides and characterization of a related cDNA clone[J]. Plant Physiol,1994,106:521~528
[57] Kremer M G Kellogy J,Wayoner W, et al. Reduced ethylene synthesis and ripening control in tomatoes expression sadenosylmethionine hydrolase. NATO ASI Ser,1997,34:307~319
[58] Downs C G,Almira E C. A beta-galactosidase cDNA homolog from broccoli. Plant Physiol,1995,108:1342~1343
[59] Carey A T,Hott K,Picard S,et al. Tomato exo-(1,4) β-D-galactosidase isolation change during ripening in normal and mutant tomato fruit, and characterization of a related cDNA clone. Plant Physiol,1995,108:1099~1107
[60] Raghothama K G,Lowton K A,Goldborough P B,et al. Characterization of an ethylene regulated flower senescence-related gene from carnation. Plant Mol Bilo,1991,17:61~71
[61] 陈昆松,李芳,张上隆.猕猴桃果实成熟进程中木葡聚糖内糖转移酶 mRNA 水平的变化.植物学报,1999,41(11):1231~1234
[62] Siddiqui S,Bangerth F. Effect of pre-harvest application of calcium on flesh firmness and cell-wall composition of apples-influnce of fruit size. J Hortic Sci,1995,70:263~269
[63] King G A, Davies M. Cloning of a harvest-inducedβ-galactosidase from tips of harvest asparagus spears. Plant Physiol,1995,108:419~420
[64] Downs C G, Almira E C. A beta-galactosidase cDNA homolog from broccoli. Plant Physiol,1995,108:1342~1343
[65] Sisler EC. Ethylene activity of some pi acceptor compounds. Tobacco Sci,1977,2
[66] Sisler EC,Serek M. Inhibitors of ethylene responses in plants at the receptor level: resent development. Physiol Plant,1997,100:577~82
[67] Sisler EC. Ethylene-binding compenets in plants. In: Mattoo AK,Suttle JC (end). The Plant Hormone Ethylene. Boca Raton: CRC Press,1991,81~89
[68] Sisler EC. Ethylene activity of some pi acceptor compounds [J]. Tobacco Sci,1977,21:43~45
[69] Serek M, Sisler EC, Reid MS. Novel gaseous inhibitor of ethylene binding prevents ethylene effects in potted flowering plant [J]. J Amer Soc HortSci,1994,119:1230~1233
[70] Jeong J, Huber D J, Sargent S A. The potential benefits of 1-MCP for regulating the ripening and extending the storage of avocados[J] .HortSci,1999,34(3):538
[71] Serek M, Sisler EC, Reid MS. Effect of 1-MCP on vase life and ethylene response of cut flowers. Plant Grow Regul,1995,16:93~97
[72] Serek M, Dupille E, Sisler EC. Effect of 1-methylcyclopropene and methylenecyclopropene on ethylene binding and ethylene action on cut flower. Plant Grow Regul,1996,18:79~86
[73] Jiang Y M, Joyce D C, Macnish A J. Response of banana fruit to treatment with 1-methylcyclopropene. Plant Grow Regul,1999,77~82
[74] Mathooko FM, Tsunashima Y, Owino WZO. Regulation of genes encoding ethylene biosynthetic e nzymes in peach fruit by carbon dioxide and l-methylcyclopropene [J]. Postharvest Biol Technol, 2001,14:265~281
[75] Lelievre J M,Latche A,Bouzyen M,et al. Ethylene and fruit ripening [J]. Plant Physiol.,1997, 101:727~739
[76] Nakatsuka A, Murachi S, Okunishi H, et al. Differential expression and internal feedback regulation of 1-amiocyclopane-1-carboxylate synthase, 1-amiocyclopane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening [J]. Plant Physiol , 1998. 118:1295-1305.
[77] Macnish AJ, Joyce DC, Hofman PJ, et al.. 1-methylcyclopropene treatment efficacy inpreventing ethylene perception in banana fruit and grevillea and waxflower flowers. AusJ. Expl Agric,2000a,40:471~481
[78] Mitcham E , Bower J H , Biasi W V . Effects of repeated application of low concentrations of 1-MCP on storage and ripening of ‘bartlett’ pears. See: ⅩⅩⅥth International Horticultural Congress (Abstracts). Toronto Ganada. 2002. 236
[79] Kim HO, Hewett EW, Lallu N. Softening and ethylene production of kiwifruit reduced with 1-methylcyclopropene. Acta Hort. 2001,553:167~170
[80] Ku VVV, Wills R B H,Ben-Yehoshua S. 1-Methylcyclopropene can differentially affect the postharvest life of strawberries exposed to ethylene. HortiSci,1999,34(1):119~120
[81] Blankship SM, Dole JM. 1-methylcyclopropene: a review. Postharvest Biol. Technol.2003,28:1~25
[82] Fan X, Mattheis JP . Development of apple superficial scald, soft scald, core flush, and greasiness is reduced by MCP. J. Agric. Food Chem. 1999a,47:3063~3068
[83] Jiang YM , Joyce DC , Macnish A J . Effect of abscisic acid on banana fruit ripening inrelation to the role of ethylene. J. Plant Growth Regul.,2000,19:106~111
[84] Sisler E , Dupille E ,Serek M . Effect of 1-methylcyclopropene and methylcyclopropane on ethylene binding and ethylene action on cut carnations. Plant Grow Regul,1996a,18:79~86
[85] Wills RBH,Ku VVV. Use of 1-MCP to extend the time of ripening of green tomatoes and postharvest life of ripening tomatoes. Postharvest Biol. Technol. 2002,26:85~90
[86] Reid M, Dodge L, Joyce D C. Environmental effects on the inhibition of ethylene action by 1-MCP. Biology and Biotechnoligy of the Plant Hormone Ethylene. NATO Advanced Workshop, June 9~13, Creat, Greece. 1996.57
[87] Halevy A H,Mayak S. Senescence and posthavest physiology of cut flowers, Part 2. Hort Rew, 1981, 3:59~143
[88] Jiang Y, Joyce D C, Macnish A J. Extension of the shelf life of banana fruit by 1-methylcyclopropene in combination with polyethylene bags [J]. Postharvest Biol Technol, 1999, 16(2):187~193
[89] Mir NA, Curell E, Khan N, Whitaker M, Beaudry RM. Harvest mature, storage temperature, and 1~MCP application frequency after firmness retention and chlorophyllfluorescence of “Redchief Delicious” apples [J] Am. Soc. Hort. Sci. 2001,126:618~624
[90] Fan X, Argenta L, Mattheis JP. Interactive effects of 1-MCP and temperature on ‘Elberta’ peach quality[J]. HortScience. 2002,37(7):134~138
[91] Dauny PT, Joyce DC. 1-MCP improves storability of ‘Queen Cox’ and ‘Bramley’ apple fruit[J]. HortScience. 2002,37(7): 1082~1085
[92] Jiang YM,Joyce DC,Macnish AJ. Response of banana fruit to treatment with 1-methycyclopropene[J]. Plant Growth Regulation. 1999b,28:77~82
[93] 王文辉,孙希生,李志强,王志华,张志云.1-MCP 对软肉梨采后生理的影响[J]. 园艺学进展.2002,5:783~790
[94] Dong L, Luric S, Zhou H. Effects of 1-methylcyclopropene on ripening of “Canino”apricots and “Royal Zee” plums[J]. Postharvest Biol. Technol. 2002,24: 135~145
[95] Porat R, Weiss B, Cohen L, Avinoam D, et al.. Effects of ethylene and1-methylcyclopropene on the postharvest qualities of ‘shamouti’ oranges[J]. PostharvestBiol and Technol,1999, 15:155~163
[96] 王文辉,王志华,李志强等. 1-MCP 对鲜枣采后生理及保鲜效果的影响[J]. 保鲜与加工,2003,3(1):21~23
[97] Hoffman PJ, Jobin-Dccor M, Macnish AJ, Joyce DC. Ripening and quality responses of avocado, custard apple, mango and papaya fruit to 1-methylcyclopropene. Aust J. Exp. Agric. 2001,41:567~572
[98] Watkins C B, Nock J F, Whitaker B D. Responses of early, mid and late season apple cultivars to postharvest application of 1-methylcyclopropene(1-MCP) under air and controlled atmosphere storage conditions [J]. Postharvest Biol Technol,2000,19(1):17~32
[99] Golding J B ,Shearer D ,Wyllie S G ,et al. Application of 1-MCP and propylene to identify ethylene-dependent ripening processes in mature banana fruit. Postharvest Bioland Technol,1998,14:87~98
[100] 吴振先,张廷亮,陈永明,季作梁,陈维信.1-甲基环丙烯处理对不同成熟阶段香蕉果实后熟的影响.华南农业大学学报.2001,22(4):15~18
[101] Nazir A Mir, Erin Curelle, Najma Khan, et al. Harvest maturity, storage temperature, and 1-MCP application frequency alter firmness retention and chlorophyll fluorescence of ‘Redchief Delicious’ apples. J Amer Hort Sci, 2001, 126(5):618~624
[102] 孙希生,王文辉,王志华等. 1-MCP 对“金冠”苹果采后生理效应的影响.园艺学进展[J].2002.5: 729~734
[103] Kremer M G, Kellogy J, Wayoner W, et al. Reduced ethylene synthesis and ripening control in tomatoes expression sadenosylmethionine hydrolase [J]. NATO ASI Ser,1997,34:307~319
[104] Wills RBH,Ku VVV. Use of 1-MCP to extend the time of ripening of green tomatoes and postharvest life of ripening tomatoes [J]. Postharvest Biol. Technol. 2002,26:85~90
[105] Fan X.T., J.P. Mattheis, and S. Blankenship. Development of apple superficial scald, soft scald,core flush, and greasiness is reduced by MCP[J]. Agri. Food Chem.,1999,47 (8):3063~3068.
[106] Fan X.T.,J.P. Mattheis. Impact of 1-methylcyclopropene and methyl jasmonate on apple Volatile production. J. Agri,Food Chem.,1999,47 (7):2847~2853.
[107] Fan X.T., L. Argenta and JMattheis. Inhibition of ethylene action by 1-methylcyclopropene prolongs storage life of apricots. Postharvest Biol. Technol.,2000,20 (2):135~142.
[108] Fan X.T., J.P. Mattheis. Methyl jasmonate promotes apple fruit degreening independently of ethylene action[J]. Hort.Sci. 1999,34(2):310~312
[109] Song J., M.S. Tian, D.R. Dilley, et al. Effect of 1-MCP on fruit ripening and volatile production [J]. Hort. Sci.,1997,32:536.
[110] Fan X, Blankenship SM , Mattheis JP. 1-methylcyclopropene inhibits apple ripening [J]. Amer. Soc. Sci., 1999,124(6):690~695
[111] 孙希生,王文辉,李志强等. 1-MCP 对新红星苹果保鲜效果的影响. 中国园艺学会第九届学术年会论文集[J]. 北京:中国科学技术出版社,2001,51~55
[112] 孙希生,王文辉,王志华等. 1-MCP 对苹果采后生理的影响[J]. 保鲜与加工,2002,4:3~7
[113] Gong Y,Fan X, Mattheis JP. Responses of “Bing”and “Rainier” Sweet cherries to ethylene and 1-methylcyclopropene J Am. Soc. Hort. Sci.2002,127:831~835.
[114] Fan X, Argenta L, Mattheis JP. Inhibiton of ethylene action by 1-methylcyclopropene prolongs storage life of apricots. Postharvest Biol. Technol 2000a,20:135~142
[115] Dong L, Luric S, Zhou H. Effects of 1-methylcyclopropene on ripeningof“Canino”apricotsand “Royal Zee” plums. Postharvest Biol. Technol. 2002,24: 135~145.
[116] Serek M,Reid MS. Ethylene and postharvest performance of potted kalanchoe.Postharvest Biol and Technol,2000,18:43~48.
[117] 唐燕,马书尚, 武春林. 1-MCP 对嘎拉苹果呼吸、乙烯产生及贮藏品质的影响. 果树学报, 2004, 21(1): 42~45.
[118] Mir N A, Curell E, Khan N, et al. Harvest maturity, storage temperature, and 1-MCP application frequency alter firmness retention and chlorophyll fluorescence of `Red chief delicious` apples [J]. J Amer Soc Hort Sci, 2001,126(5):618~624
[119] Mattheis J, Fan X, Argenta L. Impact of 1-MCP on climacteric fruit volatile productionand aroma. See: ⅩⅩⅥ th International Horticultural Congress (Abstracts).Toronto Ganada. 2002. 235
[120] 孙希生, 王文辉, 王志华等. 1-MCP 对“金冠”苹果采后生理效应的影响. 园艺学进展. 2002, 5: 729~734
[121] Fan X, Argenta L, Mattheis JP. Interactive effects of 1-MCP and temperature on ‘Elberta’peach quality. HortScience. 2002,37(7):134~138
[122] Fan X, Argenta L ,Mattheis JP . Impacts of ionizing radiation on volatile production by ripening gala apple fruit. J Agric.Food Chem.,2001,49:254~26
[123] Feng X,Apelbum A,Sisler EC,Goren R. Control of ethylene responses in avocado fruit with 1-methylcyclopropene. Postharvest Biol. Technol. 2000,20:143~150
[124] Mattheis J, Fan X, Argenta L. Responses of apple and pear fruit to 1-methylcyclopropene [R]. Yakima WA: Proceedings of 16th Annual Postharvest Conference, 2000
[125] Trivedi P, Matto A, Solomos T. Effects of MCP and 1.52 kpa oxygen on apple scald control and alpha-farnesene biosynthesis. See: ⅩⅩⅥth International Horticultural Congress (Abstracts). TorontoGanada. 2002. 239
[126] CrippsJEL,Richards L A,Mariata AM.‘PinkLady’apple.Hortscience,1993(10):1057 .
[127] 樊秀彩,张继澍. 1-甲基环丙烯对采后猕猴桃果实生理效应的影响[J]. 园艺学报,2001,28(5):399~402
[128] 袁景军,赵政阳,王雷存. 粉红女士苹果发展建议[J]. 西北园艺 2003 04: 5~6
[129] WANG Q M,GUO D P,KYIKYI W YUAN J..Effect of 1-MCP on Delaying the Senescence of Broccoli and Its Possible Physiological Mechanism[J].Acta Hortculturae Sinica,2004,(31):205~209.
[130] Renate M, Edwarad C M, Margrethe S. Stress induced ethylene production, ethylene binding, and the response to the ethylene action inhibitor 1-MCP in miniature roses [J]. Scientia Hoeticulturae, 2000, 83: 51~59.
[131] Nadeau J A,Zhang X S, Nair H, O’Neill S D. Temporal and spatial regulation of 1-aminocyclopropane-1-carboxylate oxidase in the pollination-induced senescence of orchid flowers [J]. Plant Physiology,1993,103:31—39
[132] 陈毓醛. 生物化学研究技术[M]. 北京: 中国农业出版社, 1993: 198~200
[133] 牛锐敏 ,饶景萍 ,韩新花等. 不同采收期对红富士苹果贮藏品质的影响[J]. 西北农业学报 2006 15(3):171~174
[134] 王赵改,马书尚,王瑞庆等.1-MCP 对粉红女士苹果采后生理的影响[J].西北农林科技大学学报,2005,33:(5)123~126
[135] E A Curry. Effect of harvest Date and Oxygen level on Storability of Late Season Apple Cultivars. Controlled Atmosphere Research Conference,1989,1:103~110
[136] Lau O L. Harvest Indices for British Columbla-grown Apples. Controled Atmosphere Research Conference,1989,1:115~120.
[137] 颜志梅,盛宝龙,蔺 经等 翠冠梨不同采收期对贮藏性的影响[J].江苏农业科学,2005,1: 100~101
[138] 孙芳娟,韩明玉,赵彩平等. 采收成熟度对油桃贮藏品质的影响[J].西北植物学报,2007,27:(1)183~185
[139] 王瑞庆, 马书尚, 武春林等. ‘嘎拉’苹果对不同浓度 1-MCP 处理的反应[J]. 西北植物学报, 2005,25(2):256~261
[140] Lay-Yee M, Dean D, Gavin S R. Changes in mRNA and protein during ripening in apple fruit (Malus domestia Borkh. cv Golden Delicious)[J]. Plant Physiology,1990,94:850—853.
[141] Eva Dominguez-Puigjaner, Miguel Vendrell, M Dolors Ludevid. Differential protein accumulation in banana fruit during ripening[J]. Plant Physiol,1992,98:157~162