过瘤胃保护胆碱的研究和开发
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摘要
本文选用乙基纤维素(EC)、壳聚糖(CS)、丙烯酸树脂Ⅳ三种壁料采用空气悬浮法制备了不同复合壁材包被的过瘤胃氯化胆碱,并利用体外模拟消化液法、瘤胃尼龙袋法和小肠冻干粉法测定了不同过瘤胃氯化胆碱产品的瘤胃释放率和小肠释放率,从而选出最佳的包被壁材,最后通过饲养试验研究了过瘤胃氯化胆碱对山羊生长性能和血液生化指标的影响。
1.试验分别选用白炭黑、环糊精做液态氯化胆碱固化造粒的载体,羧甲基纤维素钠(CMC)做粘合剂,微晶纤维素(MCC)做赋形剂,对液态氯化胆碱的最佳固化造粒工艺进行研究。试验结果表明:白炭黑吸附能力优于环糊精,白炭黑与氯化胆碱的最佳比例为1:1。
2.试验选用乙基纤维素(EC)、壳聚糖(CS)、丙烯酸树脂Ⅳ三种材料做壁材,通过空气悬浮法制备过瘤胃氯化胆碱,利用体外模拟消化液法对过瘤胃氯化胆碱的有效释放率进行检测。试验结果表明:综合比较成本和制备效果,2号产品(由内到外壁材组合:5%Ⅳ、3%CS和5%EC)和6号产品(由内到外壁材组合:5%Ⅳ、3%EC和5%CS)的过瘤胃效果优于其他产品。
3.试验通过瘤胃尼龙袋法和小肠冻干粉法测定过瘤胃氯化胆碱的瘤胃释放率和小肠释放率,研究不同壁材对过瘤胃氯化胆碱释控性能影响的研究。试验结果表明:5%丙烯酸树脂Ⅳ、3%壳聚糖(CS)、5%乙基纤维素(EC)包被的过瘤胃氯化胆碱效果最好。
4.本试验选用2年龄健康麻城黑山羊12只,采用单因子随即区组设计,按体重相近的原则,分为4组,每组3个重复,每个重复1只。对照组为基础日粮组,处理Ⅰ组为精料补充料+0.1%过瘤胃氯化胆碱组,处理Ⅱ组为精料补充料+0.2%过瘤胃氯化胆碱组,处理Ⅲ组为精料补充料+0.3%过瘤胃氯化胆碱组。试验期5周,测定了山羊的平均日采食量(ADFI)、平均日增重(ADG)和料肉比(F/G)以及山羊血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、尿素氮(BUN)、碱性磷酸酶(AKP)、甘油三酯(TG)和总胆固醇(TCH)的含量。试验结果表明:试验结果表明:在本试验的条件下,在1.2倍维持需要的基础上,生长山羊精料补充料中添加0.1%RPC后,山羊的平均日增重提高了60.29g(P＜0.05),料重比降低了7.21(P＜0.05):山羊血清中谷丙转氨酶(ALT)降低了5.83(P＜0.05)。综合比较得出过瘤胃保护胆碱产品在山羊精料补充料中的最佳添加量为0.1%。
This article optional ethylcellulose(EC),chitosan(CS),acrylic resin materialⅣused three air suspension prepared by a different compound wall material of the package was Rumen choline chloride,and use of simulation in vitro digestion Of law,Rumen nylon bags of freeze-dried powder and small intestine was determined by a different Rumen choline chloride products Rumen stability and digestibility small intestine,thus select the best package was wall material,the final adoption of a feeding trial on the rumen Choline chloride on the growth performance of goats. This research including four experiments.
1.Test were selected silicon dioxide,cyclodextrin do granulation liquid choline chloride curing the carrier,CMC(CMC) do adhesives,microcrystalline cellulose(MCC) do excipients,the liquid chlorine Choline of the best cure granulation technology research.The results showed that:silicon dioxide absorption capacity than the cyclodextrin better,the best ratio is 1:1.
2.Selection of Test selection ethylcellulose(EC),chitosan(CS),Ⅳthree acrylic resin materials,through the air suspended prepared by the rumen choline chloride,used in vitro method to simulate the digestive juice Rumen chloride Gall to release the effective rate for testing.The results showed that,comparison of costs and effects of No.2,products(from the wall to build combinations:5%Ⅳ, 3%CS and 5%EC) and 6 products(from the wall to build combinations:5%Ⅳ, 3%EC and 5%CS) in the rumen is better than other products.
3.Rumen test through nylon bags of freeze-dried powder and small intestine Determination of the rumen choline chloride Rumen stability of the small intestine and digestibility,research on a different wall material Rumen choline chloride controlled release properties of the study.The results showed that:5%acrylic resinⅣ,3%chitosan(CS),5%ethylcellulose(EC) of the package was the best Rumen choline chloride.
4.This test optional 8-month-old black goat health Macheng 12,was a one-block design,according to the principle of similar weight,divided into four groups,each with three repeat,repeat each one.The control group-based diet group, for the treatment groupⅠconcentrate supplementary materials +0.1%Rumen choline chloride group had to deal with groupⅡconcentrate +0.2%expected to add a group Rumen choline chloride,for the treatment groupⅢConcentrate supplementary material + 0.3%over the rumen choline chloride group.5-week trial period,the average of a goat on the intake(ADFI),average daily gain(ADG) and Liao Roubi(FCR) and the goat serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total Protein(TP),albumin(ALB),MG(GLO),urea nitrogen(BUN),ALP(AKP),triglycerides(TG) and total cholesterol(TCH) the content.The results showed that:The results show that:in this test under the condition of 1.2 times the need to maintain,on the basis of goats concentrate added compound add 0.1%RPC,goats,the average daily gain increased 60.29 g(P＜0.05),feed conversion ratio decreased 7.21(P＜0.05);goat serum alanine aminotransferase(ALT) decreased 5.83(P＜0.05).Draw a comparison of Rumen-protected chlorine chloride products in the rumen goat concentrate the best content compound added 0.1 percent.
1.试验分别选用白炭黑、环糊精做液态氯化胆碱固化造粒的载体,羧甲基纤维素钠(CMC)做粘合剂,微晶纤维素(MCC)做赋形剂,对液态氯化胆碱的最佳固化造粒工艺进行研究。试验结果表明:白炭黑吸附能力优于环糊精,白炭黑与氯化胆碱的最佳比例为1:1。
2.试验选用乙基纤维素(EC)、壳聚糖(CS)、丙烯酸树脂Ⅳ三种材料做壁材,通过空气悬浮法制备过瘤胃氯化胆碱,利用体外模拟消化液法对过瘤胃氯化胆碱的有效释放率进行检测。试验结果表明:综合比较成本和制备效果,2号产品(由内到外壁材组合:5%Ⅳ、3%CS和5%EC)和6号产品(由内到外壁材组合:5%Ⅳ、3%EC和5%CS)的过瘤胃效果优于其他产品。
3.试验通过瘤胃尼龙袋法和小肠冻干粉法测定过瘤胃氯化胆碱的瘤胃释放率和小肠释放率,研究不同壁材对过瘤胃氯化胆碱释控性能影响的研究。试验结果表明:5%丙烯酸树脂Ⅳ、3%壳聚糖(CS)、5%乙基纤维素(EC)包被的过瘤胃氯化胆碱效果最好。
4.本试验选用2年龄健康麻城黑山羊12只,采用单因子随即区组设计,按体重相近的原则,分为4组,每组3个重复,每个重复1只。对照组为基础日粮组,处理Ⅰ组为精料补充料+0.1%过瘤胃氯化胆碱组,处理Ⅱ组为精料补充料+0.2%过瘤胃氯化胆碱组,处理Ⅲ组为精料补充料+0.3%过瘤胃氯化胆碱组。试验期5周,测定了山羊的平均日采食量(ADFI)、平均日增重(ADG)和料肉比(F/G)以及山羊血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、尿素氮(BUN)、碱性磷酸酶(AKP)、甘油三酯(TG)和总胆固醇(TCH)的含量。试验结果表明:试验结果表明:在本试验的条件下,在1.2倍维持需要的基础上,生长山羊精料补充料中添加0.1%RPC后,山羊的平均日增重提高了60.29g(P＜0.05),料重比降低了7.21(P＜0.05):山羊血清中谷丙转氨酶(ALT)降低了5.83(P＜0.05)。综合比较得出过瘤胃保护胆碱产品在山羊精料补充料中的最佳添加量为0.1%。
This article optional ethylcellulose(EC),chitosan(CS),acrylic resin materialⅣused three air suspension prepared by a different compound wall material of the package was Rumen choline chloride,and use of simulation in vitro digestion Of law,Rumen nylon bags of freeze-dried powder and small intestine was determined by a different Rumen choline chloride products Rumen stability and digestibility small intestine,thus select the best package was wall material,the final adoption of a feeding trial on the rumen Choline chloride on the growth performance of goats. This research including four experiments.
1.Test were selected silicon dioxide,cyclodextrin do granulation liquid choline chloride curing the carrier,CMC(CMC) do adhesives,microcrystalline cellulose(MCC) do excipients,the liquid chlorine Choline of the best cure granulation technology research.The results showed that:silicon dioxide absorption capacity than the cyclodextrin better,the best ratio is 1:1.
2.Selection of Test selection ethylcellulose(EC),chitosan(CS),Ⅳthree acrylic resin materials,through the air suspended prepared by the rumen choline chloride,used in vitro method to simulate the digestive juice Rumen chloride Gall to release the effective rate for testing.The results showed that,comparison of costs and effects of No.2,products(from the wall to build combinations:5%Ⅳ, 3%CS and 5%EC) and 6 products(from the wall to build combinations:5%Ⅳ, 3%EC and 5%CS) in the rumen is better than other products.
3.Rumen test through nylon bags of freeze-dried powder and small intestine Determination of the rumen choline chloride Rumen stability of the small intestine and digestibility,research on a different wall material Rumen choline chloride controlled release properties of the study.The results showed that:5%acrylic resinⅣ,3%chitosan(CS),5%ethylcellulose(EC) of the package was the best Rumen choline chloride.
4.This test optional 8-month-old black goat health Macheng 12,was a one-block design,according to the principle of similar weight,divided into four groups,each with three repeat,repeat each one.The control group-based diet group, for the treatment groupⅠconcentrate supplementary materials +0.1%Rumen choline chloride group had to deal with groupⅡconcentrate +0.2%expected to add a group Rumen choline chloride,for the treatment groupⅢConcentrate supplementary material + 0.3%over the rumen choline chloride group.5-week trial period,the average of a goat on the intake(ADFI),average daily gain(ADG) and Liao Roubi(FCR) and the goat serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total Protein(TP),albumin(ALB),MG(GLO),urea nitrogen(BUN),ALP(AKP),triglycerides(TG) and total cholesterol(TCH) the content.The results showed that:The results show that:in this test under the condition of 1.2 times the need to maintain,on the basis of goats concentrate added compound add 0.1%RPC,goats,the average daily gain increased 60.29 g(P＜0.05),feed conversion ratio decreased 7.21(P＜0.05);goat serum alanine aminotransferase(ALT) decreased 5.83(P＜0.05).Draw a comparison of Rumen-protected chlorine chloride products in the rumen goat concentrate the best content compound added 0.1 percent.
引文
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