湖南柑橘种质资源评价及主要病害分子鉴定
|
摘要
湖南地处南亚热带至温带过渡地带,是柑橘种质资源分布极为丰富的地区之一。在长期的自然演变和栽培驯化过程中,形成了极为丰富的柑橘属种群和变异类型。以往对湖南柑橘种质资源尚缺乏系统的研究。本文在前期研究的基础上,采用植物形态学与分子生物学相结合的方法,对湖南柑橘种质资源分布、种群特征;主要种质资源遗传多样性及主要柑橘病毒类病害的分子鉴定等方面进行了研究。以期为柑橘资源的合理利用和确立南岭山脉宽皮柑橘近缘野生种在柑橘起源和分类中的地位提供科学依据。
主要研究结果如下:
1、在长期的自然演化、天然杂交、自然突变、引种驯化过程中,湖南境内形成了极为丰富的柑橘属种群和变异类型。通过实地调查结合查阅资料、群众申报,对湖南地方柑橘资源进行了收集、整理和归类,按照施文格(Swingle)分类方法分为枳属(Poncirus)、金柑属(Fortunella)和柑橘属(Citrus),共11种。根据湖南省柑橘种质资源自然演化和区域分布特征将地方柑橘种质资源划分为三个柑橘种质资源区。即南岭山脉野生柑橘种质资源区,武陵山脉野生柑橘、天然杂交种种质资源区和雪峰山脉天然杂种、自然突变种质资源区。
2、发掘、收集、选育了一批具有地方特色的柑橘种质资源,并对其潜在利用价值进行了初步评价。南岭山脉宽皮柑橘近缘野生资源类主要有莽山野橘、道县野橘、江永野橘;适宜作新品种培育的优良变异有红皮冰糖橙、大果冰糖橙、冰糖脐橙、红橙1号、浦市无核甜橙、溆浦长形少核甜橙、无核朱红桔、慈利绿肉柚、安江红心柚、安农2号少核蜜柚、金皮蜜柚、洪江冰糖柚、菠萝香柚、慈利杂柑;其中红皮冰糖橙、大果冰糖橙2006年经专家审定登记为新品种。适宜鲜食或加工用的地方品种有永顺蜜橘、安江香柚、大庸菊花心柚、慈利金香柚、早甜柚、浏阳金柑、蓝山金柑、大红甜橙、慈利香橙、黄皮酸橙;可供砧木选择的资源有永顺枳橙、山金柑、宜昌橙、桔红;适宜作育种材料的特异种质有洞口不苦枳、葫芦橘、湘慈33号。
3、为丰富湖南柑橘种质资源,近年从中国柑橘研究所、华中农业大学引进25个国外柑橘优良新品种,集中定植在安化柑橘良繁场进行引种栽培试验,通过生物学特性、产量、品质和生态适应性评价,从中筛选适合本省栽培的品种有纽荷尔新系、红肉脐橙、福本、耐湿脐橙、日南一号、克里曼丁勃那特、天草、秋辉、诺瓦、HB柚、地中海橘、埃及糖橙,其中已有6个品种获湖南省农作物品种登记。
4、采用AFLP技术,对湖南省南岭山脉宽皮橘近缘野生资源、莽山野橘、道县野橘、江永野橘及印度野橘、立花橘等23个材料进行AFLP多态性鉴定。从64对引物组合中,筛选了8对引物组合,共得到334个扩增位点、4612条带数据,其中多态性位点294个,多态性位点比率88%。采用DICE相似系数UPGMA方法聚类,进行亲缘关系分析,23个材料多态性带比率为0.729～0.850。相似系数为0.68～0.91。根据聚类结果,结合植物学性状、花粉形态分析,表明莽山尖叶和圆叶型野橘、道县粗皮小果型野橘、江永粗皮小果型野橘与印度野橘、立花橘亲缘关系较远,是完全不同的宽皮柑橘近缘野生种。道县野橘与江永野橘亲缘关系相对较近,应是同一种的不同地域类型。根据本研究结果,综合前人在形态学、化学成分分析及同工酶等方面的研究,参考历史、生物地理学资料,认为南岭山脉不仅具有真正柑橘亚属宽皮柑橘近缘野生种,而且柑橘近缘野生种具有丰富的遗传多样性。当地存在的不同宽皮橘类古老栽培品种,极有可能是起源于不同类型的宽皮柑橘类野生资源。南岭山脉一带是真正柑橘亚属的起源中心。
5、利用AFLP分析鉴定了从湖南地方柑橘品种中选出的7个柑橘变异类型,从64对引物组合中筛选了8对引物组合,共得到334个扩增位点2099条带数据。7个变异类型与亲本品种之间多态性带为36至92条,变异类型的多态性带数与亲本品种总带数的比例为0.1721至0.4182。说明变异类型的遗传基础已经发生不同程度的改变。7个柑橘变异类型应是芽变,具备了新品种的遗传基础。研究同时表明,AFLP具有扩增谱带多,谱带清晰,扩增信息量大的特点。AFLP是柑橘新品种选育早期鉴定灵敏、可靠、有效的方法。
6、根据国内外柑橘病毒病蔓延的趋势,为加强资源的保护利用,对四种主要柑橘病毒类病害进行了分子鉴定。全省22个柑橘主产区48个样品进行黄龙病分子检测表明,湘南主产区资兴、宜章、临武、永州、道县、祁阳等地以及湘北桃源、华容、岳阳等县市的个别产地已感染黄龙病,PCR带谱大小为563 bp,与阳性对照相同。通过测序和序列分析,检测出的柑橘黄龙病与Genbank中基因序号为(AY342001.1)亚洲黄龙病序列同源性为99.8%,属柑橘黄龙病亚洲种中的一员。与20世纪80年代黄龙病调查结果相比,发现湖南省柑橘黄龙病分布范围有所扩大,以往认为柚类不易感病,本次检测结果表明,椪柑、温州蜜柑、脐橙、琯溪蜜柚等柑橘品种均能感染黄龙病。湘北地区是首次发现黄龙病,但在当地未发现木虱,且同一果园未相互感染,可能从黄龙病疫区滥引种苗所致。
用CTV p25基因特异引物对不同品种、不同地域果园柑橘衰退病进行RT-PCR检测,85个检测样品有72个样品感染了衰退病,感染率为84.7%。说明绝大部分柑橘品种都能感染CTV,通过P25/HinfⅠRFLP分析,发现大部分感染品种的CTV株系既有单一组群又有混合组群,单一组群为1或3组群为主,混合组群以1,3混合为主,存在复杂的株系分化和混合感染。
利用一步法RT-PCR技术对湖南柑橘资源的6大类15个柑橘品种54株候选植株感染CEVd和CTLV的情况进行了鉴定。结果表明只有一个样品感染了裂皮病,其余柑橘品种的53个柑橘材料均呈阴性反应,不带CEVd。且所有检测样品均不带CTLV。
根据以上检测结果,作者提出了以建立柑橘无性系保护和构建无病毒柑橘良种繁育体系为重点的防御对策。
Hunan located at transitional region between south subtropical and temperate, which exist abundant citrus native citrus resources through long term evolution and cultivation. Collection, conservation, identification, evaluation and utilization on citrus native citrus resources in Hunan is very imperative and important. This project was supported by Hunan Scientific and technological Tepartment and Hunan Agricultural Department and focus on the distribution trait, classification ,genetic diversity and major diseases on citrus native citrus resources in Hunan by means of molecular biological methods. It aimed to provide more convincing evidences for origin and taxonomy of citrus plants and supply theoretical basis for foundation of reasonable position in citrus classification system for wild citrus originated from Nanling mountain and for their conservation and utilization as well as technological foundation for cultivar registration, identification and protection in future.The main results are as follows:
1.Through long term natural evolution,hybridization, mutation,selection there are abundant native citrus germplasm resources in hunan,which are classified into Poncirus, Fortunella, Citrus genus with 11 species and distribute in Nanling mountains, Wuling mountains and Xuefeng mountains.
2. 38 native citrus germplasm resources have been collected, conserved and evaluated. Mangshanyeju, Daoxianyeju, Jiangyongyeju are relatives of Eucitrus for study of citrus classification; Bingtangchen with red rind, lager Bingtangchen , Bingtangchen with navel, seedless Zhuhongju, Cili- pumelo with green flesh, Cili- pumelo with red flesh, Annong-pumelo are bud mutants and hybids newly selected in the research;Yongshunmiju, Anjiangxiangyou, Dayongjuhuaxinyou, Cilijingxianyou, Hongjiangbi ngtangyou, Poluoxiangyou, Zaotianyou, Nanshangjingan, Cilixianchen are native cultivars with high-quality and special flavor. Yongshunzhichen, Shangjingan, Ichangenes, Juhong are suitable roostocks and resources for hybridization, Dongkoubukuzhi, Xiangci-33 are very unique germplasm resources.
3. More than 20 patented varieties are recently introduced. New Newhall, CaraCara, Fukumoto, Nova, Fallglo,HB pumelo, behave characteristics of vigorous growth habit, fair productivity and ability to bear fruit at a young age, rich ahd sweet flavor. The study analyzed and evaluated citrus quality of different varieties and made suggestion out for improving citrus structure and citrus quality regionalization in hunan and provide scientific results for construction of dominated citrus production regions.
4. AFLP technique was used to determine DNA polymorphism of the wild Mangshanyeju、Daoxianyeju、Jiangyongyeju、Citus indica and Citrus tachibana and eighteen cultivated germplasm resources of Citrus. Eight AFLP primer pairs was selected out of 64 and 334 PCR bands were obtained. Among which 294 (88%) were polymorphic. The Dice' coefficient of the samples was 0.68-0.91. the results showed Mangshanyeju、Daoxianyeju、Jiangyongyeju are different wild species of Eucitrus in comparison with Citrus indica and Citrus tachibana. Mangshanyeju is more primitive wild species than Daoxianyeju、Jiangyongyeju. The pointed leaf form and round leaf type are found in Mangshan wild mandarins. The small fruit with rough rind and large fruit with smooth rind type are found in Daoxianyeju and Jiangyongyeju. In addition to the existence and the wild distribution of wild Citrus species in Nanling Mountains plus the DNA polymorphic data, pollen morphology and taxonomic characteristics obtained in this study supported that Nanling Mountains is one of the native centers of the subgenus Eucitrus.
5. AFLP technique was used to determine DNA polymorphism of citrus mutants originated from 2different parent cultivars in Hunan province. Eight was selected out among 64 pairs of prime combinations and 2099 polymorphism bands were amplified. There are 36 to 92 polymorphism bands between mutants and parent cultivars. Ratio of polymorphism bands of every mutant to total bands of parent cultivars is 0.1721 to 0.4182. The results show that 7different type of citrus mutants are bud mutant, have got genetic basis to be selected as new variety. AFLP is characterized with abundant, clear polymorphism bands and is very effectively analysis method for identification of citrus mutants.
6. Through PCR detection, we have collected information of citrus Huanglongbing in Hunan Province. 48 samples were collecting from 22 production districts to detect Citrus huanglongbing, Citrus Huanglongbing have been detected from16 samples. The results showed that trees from eight places were infected. The places lie in Xiangnan Citrus producing district. Comparing with the condition that occurred in 1980's, it shows the tendency to spread to the north. Some orchards have citrus psyllas, which may be the major carrying medium. This research has detected citrus Huanglongbing in some orchards in Yueyang and Changde for the first time. The infected variety is only Guanximiyou pommelo. Others are not infected. There are no psyllas in Xiangbei district. The pathogen in Xiangbei may come from young trees from other provinces, where citrus trees are severely infected by Huanglongbing. Test has successfully cloned a sequence of Citrus Huanglongbing pathogen, cloned DNA was 99.8% homogous to the GenBank(AY342001.1) of Asian citrus candidates Liberibacter asiaticus, a member of L.asiaticum pathogen. CTV, CEV, CTLV have been detected simultaneously,results show 82% citrus trees were infected by CTV RFLP was used to examine the cost protein gene of CTV, and it was found that CP/Hinfl RFLP group 1,3,6 were dominant in Hunan. The results btained by RFLP analysis revealed that mixture of CTV strains often ocoured in field trees. Huanglongbing and CTV are going up as dominating Virus and viruslike diseases in Hunan. Procedure, timeline and virus-free propagation system for the introduction and propagation of new varieties should be found to support citrus industry.2
主要研究结果如下:
1、在长期的自然演化、天然杂交、自然突变、引种驯化过程中,湖南境内形成了极为丰富的柑橘属种群和变异类型。通过实地调查结合查阅资料、群众申报,对湖南地方柑橘资源进行了收集、整理和归类,按照施文格(Swingle)分类方法分为枳属(Poncirus)、金柑属(Fortunella)和柑橘属(Citrus),共11种。根据湖南省柑橘种质资源自然演化和区域分布特征将地方柑橘种质资源划分为三个柑橘种质资源区。即南岭山脉野生柑橘种质资源区,武陵山脉野生柑橘、天然杂交种种质资源区和雪峰山脉天然杂种、自然突变种质资源区。
2、发掘、收集、选育了一批具有地方特色的柑橘种质资源,并对其潜在利用价值进行了初步评价。南岭山脉宽皮柑橘近缘野生资源类主要有莽山野橘、道县野橘、江永野橘;适宜作新品种培育的优良变异有红皮冰糖橙、大果冰糖橙、冰糖脐橙、红橙1号、浦市无核甜橙、溆浦长形少核甜橙、无核朱红桔、慈利绿肉柚、安江红心柚、安农2号少核蜜柚、金皮蜜柚、洪江冰糖柚、菠萝香柚、慈利杂柑;其中红皮冰糖橙、大果冰糖橙2006年经专家审定登记为新品种。适宜鲜食或加工用的地方品种有永顺蜜橘、安江香柚、大庸菊花心柚、慈利金香柚、早甜柚、浏阳金柑、蓝山金柑、大红甜橙、慈利香橙、黄皮酸橙;可供砧木选择的资源有永顺枳橙、山金柑、宜昌橙、桔红;适宜作育种材料的特异种质有洞口不苦枳、葫芦橘、湘慈33号。
3、为丰富湖南柑橘种质资源,近年从中国柑橘研究所、华中农业大学引进25个国外柑橘优良新品种,集中定植在安化柑橘良繁场进行引种栽培试验,通过生物学特性、产量、品质和生态适应性评价,从中筛选适合本省栽培的品种有纽荷尔新系、红肉脐橙、福本、耐湿脐橙、日南一号、克里曼丁勃那特、天草、秋辉、诺瓦、HB柚、地中海橘、埃及糖橙,其中已有6个品种获湖南省农作物品种登记。
4、采用AFLP技术,对湖南省南岭山脉宽皮橘近缘野生资源、莽山野橘、道县野橘、江永野橘及印度野橘、立花橘等23个材料进行AFLP多态性鉴定。从64对引物组合中,筛选了8对引物组合,共得到334个扩增位点、4612条带数据,其中多态性位点294个,多态性位点比率88%。采用DICE相似系数UPGMA方法聚类,进行亲缘关系分析,23个材料多态性带比率为0.729～0.850。相似系数为0.68～0.91。根据聚类结果,结合植物学性状、花粉形态分析,表明莽山尖叶和圆叶型野橘、道县粗皮小果型野橘、江永粗皮小果型野橘与印度野橘、立花橘亲缘关系较远,是完全不同的宽皮柑橘近缘野生种。道县野橘与江永野橘亲缘关系相对较近,应是同一种的不同地域类型。根据本研究结果,综合前人在形态学、化学成分分析及同工酶等方面的研究,参考历史、生物地理学资料,认为南岭山脉不仅具有真正柑橘亚属宽皮柑橘近缘野生种,而且柑橘近缘野生种具有丰富的遗传多样性。当地存在的不同宽皮橘类古老栽培品种,极有可能是起源于不同类型的宽皮柑橘类野生资源。南岭山脉一带是真正柑橘亚属的起源中心。
5、利用AFLP分析鉴定了从湖南地方柑橘品种中选出的7个柑橘变异类型,从64对引物组合中筛选了8对引物组合,共得到334个扩增位点2099条带数据。7个变异类型与亲本品种之间多态性带为36至92条,变异类型的多态性带数与亲本品种总带数的比例为0.1721至0.4182。说明变异类型的遗传基础已经发生不同程度的改变。7个柑橘变异类型应是芽变,具备了新品种的遗传基础。研究同时表明,AFLP具有扩增谱带多,谱带清晰,扩增信息量大的特点。AFLP是柑橘新品种选育早期鉴定灵敏、可靠、有效的方法。
6、根据国内外柑橘病毒病蔓延的趋势,为加强资源的保护利用,对四种主要柑橘病毒类病害进行了分子鉴定。全省22个柑橘主产区48个样品进行黄龙病分子检测表明,湘南主产区资兴、宜章、临武、永州、道县、祁阳等地以及湘北桃源、华容、岳阳等县市的个别产地已感染黄龙病,PCR带谱大小为563 bp,与阳性对照相同。通过测序和序列分析,检测出的柑橘黄龙病与Genbank中基因序号为(AY342001.1)亚洲黄龙病序列同源性为99.8%,属柑橘黄龙病亚洲种中的一员。与20世纪80年代黄龙病调查结果相比,发现湖南省柑橘黄龙病分布范围有所扩大,以往认为柚类不易感病,本次检测结果表明,椪柑、温州蜜柑、脐橙、琯溪蜜柚等柑橘品种均能感染黄龙病。湘北地区是首次发现黄龙病,但在当地未发现木虱,且同一果园未相互感染,可能从黄龙病疫区滥引种苗所致。
用CTV p25基因特异引物对不同品种、不同地域果园柑橘衰退病进行RT-PCR检测,85个检测样品有72个样品感染了衰退病,感染率为84.7%。说明绝大部分柑橘品种都能感染CTV,通过P25/HinfⅠRFLP分析,发现大部分感染品种的CTV株系既有单一组群又有混合组群,单一组群为1或3组群为主,混合组群以1,3混合为主,存在复杂的株系分化和混合感染。
利用一步法RT-PCR技术对湖南柑橘资源的6大类15个柑橘品种54株候选植株感染CEVd和CTLV的情况进行了鉴定。结果表明只有一个样品感染了裂皮病,其余柑橘品种的53个柑橘材料均呈阴性反应,不带CEVd。且所有检测样品均不带CTLV。
根据以上检测结果,作者提出了以建立柑橘无性系保护和构建无病毒柑橘良种繁育体系为重点的防御对策。
Hunan located at transitional region between south subtropical and temperate, which exist abundant citrus native citrus resources through long term evolution and cultivation. Collection, conservation, identification, evaluation and utilization on citrus native citrus resources in Hunan is very imperative and important. This project was supported by Hunan Scientific and technological Tepartment and Hunan Agricultural Department and focus on the distribution trait, classification ,genetic diversity and major diseases on citrus native citrus resources in Hunan by means of molecular biological methods. It aimed to provide more convincing evidences for origin and taxonomy of citrus plants and supply theoretical basis for foundation of reasonable position in citrus classification system for wild citrus originated from Nanling mountain and for their conservation and utilization as well as technological foundation for cultivar registration, identification and protection in future.The main results are as follows:
1.Through long term natural evolution,hybridization, mutation,selection there are abundant native citrus germplasm resources in hunan,which are classified into Poncirus, Fortunella, Citrus genus with 11 species and distribute in Nanling mountains, Wuling mountains and Xuefeng mountains.
2. 38 native citrus germplasm resources have been collected, conserved and evaluated. Mangshanyeju, Daoxianyeju, Jiangyongyeju are relatives of Eucitrus for study of citrus classification; Bingtangchen with red rind, lager Bingtangchen , Bingtangchen with navel, seedless Zhuhongju, Cili- pumelo with green flesh, Cili- pumelo with red flesh, Annong-pumelo are bud mutants and hybids newly selected in the research;Yongshunmiju, Anjiangxiangyou, Dayongjuhuaxinyou, Cilijingxianyou, Hongjiangbi ngtangyou, Poluoxiangyou, Zaotianyou, Nanshangjingan, Cilixianchen are native cultivars with high-quality and special flavor. Yongshunzhichen, Shangjingan, Ichangenes, Juhong are suitable roostocks and resources for hybridization, Dongkoubukuzhi, Xiangci-33 are very unique germplasm resources.
3. More than 20 patented varieties are recently introduced. New Newhall, CaraCara, Fukumoto, Nova, Fallglo,HB pumelo, behave characteristics of vigorous growth habit, fair productivity and ability to bear fruit at a young age, rich ahd sweet flavor. The study analyzed and evaluated citrus quality of different varieties and made suggestion out for improving citrus structure and citrus quality regionalization in hunan and provide scientific results for construction of dominated citrus production regions.
4. AFLP technique was used to determine DNA polymorphism of the wild Mangshanyeju、Daoxianyeju、Jiangyongyeju、Citus indica and Citrus tachibana and eighteen cultivated germplasm resources of Citrus. Eight AFLP primer pairs was selected out of 64 and 334 PCR bands were obtained. Among which 294 (88%) were polymorphic. The Dice' coefficient of the samples was 0.68-0.91. the results showed Mangshanyeju、Daoxianyeju、Jiangyongyeju are different wild species of Eucitrus in comparison with Citrus indica and Citrus tachibana. Mangshanyeju is more primitive wild species than Daoxianyeju、Jiangyongyeju. The pointed leaf form and round leaf type are found in Mangshan wild mandarins. The small fruit with rough rind and large fruit with smooth rind type are found in Daoxianyeju and Jiangyongyeju. In addition to the existence and the wild distribution of wild Citrus species in Nanling Mountains plus the DNA polymorphic data, pollen morphology and taxonomic characteristics obtained in this study supported that Nanling Mountains is one of the native centers of the subgenus Eucitrus.
5. AFLP technique was used to determine DNA polymorphism of citrus mutants originated from 2different parent cultivars in Hunan province. Eight was selected out among 64 pairs of prime combinations and 2099 polymorphism bands were amplified. There are 36 to 92 polymorphism bands between mutants and parent cultivars. Ratio of polymorphism bands of every mutant to total bands of parent cultivars is 0.1721 to 0.4182. The results show that 7different type of citrus mutants are bud mutant, have got genetic basis to be selected as new variety. AFLP is characterized with abundant, clear polymorphism bands and is very effectively analysis method for identification of citrus mutants.
6. Through PCR detection, we have collected information of citrus Huanglongbing in Hunan Province. 48 samples were collecting from 22 production districts to detect Citrus huanglongbing, Citrus Huanglongbing have been detected from16 samples. The results showed that trees from eight places were infected. The places lie in Xiangnan Citrus producing district. Comparing with the condition that occurred in 1980's, it shows the tendency to spread to the north. Some orchards have citrus psyllas, which may be the major carrying medium. This research has detected citrus Huanglongbing in some orchards in Yueyang and Changde for the first time. The infected variety is only Guanximiyou pommelo. Others are not infected. There are no psyllas in Xiangbei district. The pathogen in Xiangbei may come from young trees from other provinces, where citrus trees are severely infected by Huanglongbing. Test has successfully cloned a sequence of Citrus Huanglongbing pathogen, cloned DNA was 99.8% homogous to the GenBank(AY342001.1) of Asian citrus candidates Liberibacter asiaticus, a member of L.asiaticum pathogen. CTV, CEV, CTLV have been detected simultaneously,results show 82% citrus trees were infected by CTV RFLP was used to examine the cost protein gene of CTV, and it was found that CP/Hinfl RFLP group 1,3,6 were dominant in Hunan. The results btained by RFLP analysis revealed that mixture of CTV strains often ocoured in field trees. Huanglongbing and CTV are going up as dominating Virus and viruslike diseases in Hunan. Procedure, timeline and virus-free propagation system for the introduction and propagation of new varieties should be found to support citrus industry.2
引文
[1]陈竹生.柑橘品种改良进展及对我国柑橘品种结构调整的建议[J].中国南方果树,2005,34(2):23-26
[2]叶荫民.柑橘品种改良的世纪回顾与展望[J]中国南方果树.2000,29(3):15-17
[3]伊华林.美国柑橘遗传资源收集保存和和评价体系[J].中国南方果树,2003,32(4):16-20
[4]刘新录,王小兵,邓秀新.巴西柑橘考察报告[J].中国南方果树,2003,32(5):20-26
[5]陈竹生.我国柑橘种质保存评价利用现状分析[J].中国南方果树,2000,29(1):14-15
[6]湖南省园艺研究所.湖南野生柑橘资源的初步调查简报[C].湖南园艺科技资料果树专辑(1975-1976),1976,P.8-10
[7]贺善文.柑橘类种质资源中心问题的初步探讨[J].园艺学报,1978,6(1):19-25
[8]李文斌,贺善文,刘庚峰.湖南野生种类种质过氧化物酶同工酶分析[J].园艺学报,1987,14(3):151-160
[9]Swingle.W.T.and Teece,P.C.The botany and citrus and its wild relatives[M].The Citrus Industry,1967,Vol.1:190-430.Berkeley:Division of Agricultural Sciences,University of California.
[10]Barrett.H.C.Intergeneric hybridization of citrus and other genera in citrus variety improvement.[J].Proc.Int,Soc.Citriculture 1977,PP.586-9.LakeAlfred,FL:ISC.
[11]Hodgson,R.W.Taxonomy and nomenclature in Citrus[J].Int.Org.Citrus Virol.Proc,1961,2:1-7
[12]贺善文.中国柑橘分类苦干问题[J].中国果树,1962,(2):28-30
[13]曾勉.对柑橘分类的认识体系和整理意见[J].中国果树,960,(2):31-37
[14]蒋聪强.论柑橘三属左右线分类系统[J].西南农学院学报,1983,(4):10-23
[15]林正奎.柑橘属11个类群叶精油成分与系统进化关系[J].植物学报,1992,34(2):133-139
[16]H.C.Dass,et al.Phylogenetic studies in Citrus reticulata and related type.[J].Euphytica,1977,26:805-810
[17]H.C.Dass,et al,Leaf flavonoid Patterns of some important citrus speeies.[J].Ind.J.Exp.Bilo,1978,16:62-65
[18]A.Esen et al.Distribution of enzymatic browning of young shoot homogenates in the Aurantinoideae.[J].Amer.J.Bot.,1975,62(10):1078-1803
[19]A.Esen et al.A simple and rapid screening procedure for identification of zygotic citrus seedlings among crosses of certain taxa[J].Amer.Soc.Hort.Sci.,1975,100(5):558-561
[20]A.Esen et al.Seperation of nucellar and zygotic citrus seedlings by use of polyphenoloxidase catalyzed browning[J].Proc.Int.Soc.Citriculture,1977,2:616-618
[21]朱立武.中国柑橘数量化学分类研究.植物分类学报,1988,26(5):353-361
[22]A.M.Torrse,et al.Leaf isozymes as genetic markers in citrus[J].Amer.J.Bot.,1978,65:869-881
[23]R.K.Soost,et al.Leat isozymes as genetic markers in citrus[J].Proc.Int.Soc.Citriculture,1981,1:7-10
[24]吴安仁.用过氧化物同工酶对柑橘分类的探讨[J].园艺学报,1985,12(2):83-87
[25]李文斌,贺善文.湖南野生柑橘类种质过氧化物酶同工酶的分析[J].园艺学报,1987.14(3):153-160
[26]廖映玢.柑橘近缘植物酯酶同工酶的研究[J].植物学报,1988,30(2):163-168
[27]钟广炎,叶荫民.柑橘过氧化物酶同工酶的遗传规律[J].中国柑橘,1989,19(3):22-24
[28]方德秋,章文才.我国果树同工酶研究现状及展望[J].福建果树,1989,(4):16-20
[29]方德秋,章文才.同工酶研究在柑橘上的应用[J].湖北农业科学,1990,(7):23-24
[30]方德秋,章文才.应用同工酶进行宽皮柑橘分类及其进化研究[J].武汉植物学研究,1992,10(4):305-312
[31]方德秋,章文才.柑橘分类研究的新方法[J].果树科学,1993,10(增刊):29-33
[32]叶荫民,孙炎,郑向红.柑橘花粉形态研究[J].中国农业科学,1982,(5):62-65
[33]熊兴耀,魏文娜,李润唐.柑橘染色体组型及Giemsa带型的研究初报[J].湖南农学院学报,1982,(2):61
[34]梁国鲁.柑橘类的细胞分类学研究[J].武汉植物学研究,1990,8(1):1-7
[35]梁国鲁,殷智勇.宽皮柑橘两个分类群的核型及C带带型分析[J].西南农大学报,1992,14(4):320-322
[36]陈喜文,陈梦龙.湖南柑橘资源的植物血清学研究[J].湖南农学院学报,1991,17(4):714-719
[37]尹佟明,黄敏仁.AFLP分子标记及其在育种上的应用[J].生物工程进展,1997,17(1):6-11
[38]吴俊,魏钦平.分子标记及其在果树种质资源研究中的应用[J].安徽农业大学学报,2002,29(2):158-162
[39]Williams,J.G.K.,Kubelik A.R.,Livak K.J.,Rafalski J.A.& TingeyS.V.,O.DNA polymorphisms amplified by arbitrary primers as useful as genetic markers.[J].Nucleic Acid Res,1990,18:6531-6535
[40]Paran,I.and R.W.Michelmore.Development of reliablePCR-based markers linked to downy mildew resistance genes inlettuce.[J].Theor.Appl.Gen.1993,85:985-993
[41]Jung,G.,P.W.Skroch,J.Nienhuis,D.P.Coyne,E.Arnaud-Santana,H.M.Ariyarathne,and J.M.Marita.Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean[J].Crop Sci.1999,39:1448-1455
[42]Hernandez,P.,A.Martin,and G.Dorado,Development of SCARs by direct sequencing of RAPD products:a practical tool for the introgression and marker-assisted selection of wheat[J].Mol.Breed.1999,5:245-253
[43]Vos.P,Hogers R,Blecker M,etal.AFLP:a new technique for DNA fingerprinting[J].Nucl Acids Res,1995,23(21):4407-4414
[44]Cho YG,Blair MW,Panaud O,et al.cloning and mapping of variety specific rice genomic DNA sequences:Amplified Fragment Length Polymorphism(AFLP)from silver-stained polyacrylamide gels.[J].Genome,1996,39:373-378
[45]Donini P,Elles ML,Bongound SM,et al.(AFLP)fingerprinting reveals pattewn differences between template DNA extracted from different plant organs[J].Genome,1997,40:521-526
[46]Stella M,Resmon-Buttner JS,Christian J,et al.AFLP Markers tightly linked to the sex locus in Asparagus officinalis[J].Molecular Breeding,1998,4:91-98
[47]Beeker J,Vos P,Kupr M,et al.Combined mapping of AFLP and RFLP markers in barley.[J].Mol.Gen Genet,1995,249:65-73
[48]Maria Teresa Cervera,Narijke Steenackers,et al.AFLP molecular markers for resistance to melamp soralarici-populina in poplar.Environmental and social issues in Poplar and Willow cultivation and utilization,1996,Vol 1,142-150
[49]Cervera M T,et al.Identification of AFLP molecular markers for resistance against Melampsora larici-populina in populus[J].Theor Appl Genet,1996,9.(5-6):733-737
[50]Hyman BC,et al.Application of mitochondrial DNA polymorphism to Meloidogyne molecular population biology[J].Nematology,1996,28(3):268-276
[51]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20:176-183
[52]Yamamoto M.et al.Polygenic relationships of Citrus reveaded by RFLP analysis of mitochon drial and chloroplast DNA[J].Japan J.Breed.1993,43:355-365
[53]熊光明,梁国鲁,吴清.适于AFLP分析用的柑橘DNA提取方法[J].果树学报,2002,19(4):247-251
[54]范眸天,高俊,吴兴恩.十五种柑橘种质资源的RAPD分析[J].中国南方果树,2002:
[55]Ros A C.Maya MM,Duque M C,et al.AFLP analysis of relationships among cassava and other Manihot species[J]Theoretical and Applied Genetics,1997,95(5-6):741-750
[56]庞晓明,胡春根,邓秀新.AFLP标记在果树遗传研究中的应用[J].生命的化学,2001,21卷第5期
[57]Aggarwal RK,Brar DS,Nandi S,Huang N,Khush GS.Phylogenetic relationships among Oryza species revealed by AFLP markers.[J].Theor Appl Genet,1999,98:1320-1328
[58]Sharma SK.Knox MR.Ellis THN.AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis[J].Theor Appl Genet.1996,93:75
[59]ZabearM,Vos P.Selective restriction fragment amplification,ageneral method for DNA finger printings[J]European Patent Applification,Publication No:858Al,1993.
[60]Vos.P,Hogers R,Blecker M,etal.AFLP:a new technique for DNA fingerpri nting[J].Nucl Acids Res,1995,23(21):4407-4414
[61]徐红梅,秦岭.AFLP技术及其在果树研究中的应用进展[J].北京农学院学报,2003,18(4):308-310
[62]Lin Jhy-jhu,et al.Identification of molecular markers in soybean comparing RFLRRAPD and AFLP DNA mapping techniques[J].Plant Molecular Biology Reporter,1996,14(2):156-169
[63]Mibourne E,et al.Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato[J].Molecular Breeding,1997,3(2):127-136
[64]International plant genome conference Ⅲ January,1995,SanDicgo,USA
[65]陶文静,刘大钧.AFLP技术及其在植物基因组研究中的应用[J].世界农业,1998,12(总57-236)1-758
[66]陈秀蕙.菠萝DNA导入黄瓜的初步研究[J].海南大学学报自然科学版,1998,16(1):62-68
[67]M.F.DuvalJ.L.Noyer · X.PerrierG Coppens d'Eeckenbrugge · P.Hamon.Molecular diversity in pineapple assessed by RFLP markers[J].Theor Appl Genet(2001)102:83-90
[68]Claudete de Fatima Ruas,Paulo Maurlcio Ruas.Assessment of genetic relatedness of the genera Ananas and Pseudananas confirmed by RAPD markers[J].Euphytica 2001.119:245-252
[69]易干军,于晓英,霍合强等.应用AFLP进行香牙蕉品种(系)的鉴别与分类[J].果树学报,2002,19(4):247-251
[70]Lu ZX,et al.Construction of a gentic linkage map and identification of AFLP marke rs for resistance to root-knot nematodes in peach rootstocks[J].Gemome,1998,41(2):199-207
[71]Harvey C.F,Gill GP,Fraser L.G and Mc Neilage M.A.Sex determination in Actinidia.sex-linked markers and progeny sex ratio in diploid.A chinensis[J].Sex Plant Report,1997,10:149-154
[72]张潞生,李传友.猕猴桃雌雄性别的AFLP标记初报[C].武汉:1998国际猕猴桃研讨会论文集,1998,17-18
[73]Testolin R,Huang W-G,Cipriani G.Towards a linkage map in kiwifruit based on microsatellites and saterated with AFLP markers[J].Acta Hort,1999,48/98:79-84
[74]TRAVIS.SE,MASCHINIKIJ,LEIM P.An analysis of biological variation in Asstragalus crem nophylax var.crem nophylax,a critically endangered plant.using AFLP markers[J].Mol Ecol,1996,5 6:735-745
[75]章一华.植物遗传资源研究的一个新领域-核心样品[J].作物品种资源,1993(2):1-3.
[76]LEFEBVRF V,GOFFINET B.CHAUVET JC,etal.Evaluation of Genetic distances between pepper inbred lines for cultivar protection purposes:comparison of AFLP,RAPD and phenotypic data[J].The or ApplGenet,2001,102:741-750
[77]房经贵,马正强.AFLP标记在两个芒果品种间杂交F1代的多态性及分离方式[J].中国农业科学,2000,33(3):19-24
[78]Waugh R,Bonar N,etal.Homology of AFLP products in three mapping populations of barley.[J].Mol Gen Genet,1997,255:311-321
[79]Sosinki B,Sossey-Alaoui K,etal.Use of AFLP and RFLP markers to create a combined linkage map in peach for use in marker assisted selection[J].Acta Hort,1998,465:61-68
[80]张潞生,李传友,贾建航.猕猴桃雌雄性别的AFLP鉴别中DNA模板的制备[J].果树科学,1999,16(3):171-175
[81]祝军,王涛,赵玉军.应用AFLP分子标记鉴定苹果品种[J].园艺学报,2000,27(2):102-106
[82]庞晓明,邓秀新,胡春根.枳属36份特异种质的AFLP指纹图谱构建与分析[J].园艺学报,2003,30(4):394-398
[83]史永忠,郭文武,邓秀新.柑橘RAPD技术体系建立与体细胞杂种鉴定[J].园艺学报,1998,25(2):105-110
[84]郝玉金.柑橘和平果等果树种质资源的离体保存及遗传变异[D],华中农业大学,1999:33-61
[85]CardinerSE,BassettHCM,MADie C.Isozyme,randomLy amplified polymorphic DNA and restriction fragment length polymorphism markers used to deduce aputative panent or the brabure apple[J].Amer SocHort Sci,1996,121(6):996-1001
[86]王丙旭.RAPD在梨种质资源亲缘关系和品种鉴定中的应用.[D],长春,吉林农业大学,1998
[87]柯穗,唐伟文,高乔婉.柑橘黄龙病病原物提纯方法和血清学的初步研究[J].华南农业大学学报,1989,10(1):1-8.
[88]Gamier M,MartinGros G,Bove J M.Monoclonal antibodiesagainst thebacterial-like organism associated with citrus greening disease[J].Ann Microbiol,1987,(138):639-650.
[89]Gamier M,Gao S J,He YL,et al.Study of the greening organism(GO)with monoclonal antibodies:serological identification,morphology,serotypes and purification of the GO[A].Proc 11~(th)Conf IOCV[C].Riverside IOCV,1991,428-435.
[90]Villechanoux S,Gamier M,Laigret F,etal.Detection of several strains of the bacteria-like organism of citrus greening disease by DNA probes[J]Curr Microbiol,1992,(24):89-95.
[91]Villechanoux S,The genome of the non-cultured,bactetia21ike organism of citrus associated with citrus greening disease contains the nusGrplKAJLrpoBC gene cluster and gene for a bacteriophage type DNA polymerase[J].Curr Microbiol,1993,(26):161:166.
[92]Jagoueix S,Bove J M,Gamier M.The phloem-limited bacterium of greening disease of citrus is a member of thea-subdivision of the proteobacteria[J].Int J Syst Bacteriol,1994,(44):379-386.
[93]张天淼.柑橘病毒病[M].北京中国农业出版社,2000.
[94]Kapur,S.P.,Ananda,S.A.,Cheema,S.S.,Kapoor,S.K.1977.Prevalence of greening marker substances in different species of citrus.[J].Indian J.Horticult.34:205-8(Rev.Plant Pathol.60:54)
[95]王运生,戴良英,荒井答.日本柑橘黄龙病病原体电镜观察及PCR检测[J].湖南农业大学学报(自然科学版),2004,10,446-449.
[96]田亚南,柯穗,李韬等.应用电镜与PCR技术检测琯溪蜜袖黄龙病病原[J].植物病理学报.2000,30(1):76-81.
[97]田亚南,柯穗,柯冲.柑橘黄龙病诊断法的研究进展[J].福建农业学报,1998,13(1):27-35.
[98]吴世盘.柑橘黄龙病的直接荧光诊断法[J].华南农业大学学报,1987,8(2):45-48.
[99]林尤剑,高日霞.应用FBA染色法观察柑橘黄龙病病株中的病原难培养细菌[J].福建农学院学报,1990,19(1):1-6.
[100]柯穗,唐伟文,高乔婉等.柑橘黄龙病病原物提纯方法和血清学的初步研究[J].华南农业大学学报,1989,10(1):1-7.
[101]Bar-Joseph M,Roistacher C N,Garnsey S M,et al.A review on tristeza,an on going threat to citriculture[J].Proc,Int Soc Citriculture,1981,1:419-423
[102]张作芳,张成良,相宁等.禁止和限制入境植物病毒的研25-柑橘速衰病毒[J]. 植物检疫研究报告,1990:1-27
[103]Karasev A V,Boyko V P,Gowda S,et al.Conplete sequence of the citrus tristezavirus RNA genome[J].Virology,1995,208:511-520
[104]Pappu H R,Karasev A V,Anderson E J,et al.Nucleotide sequence and organization of 3' open reading frames of the citrus tristeza virus genome[J].Virology,1994,99:35-46
[105]Mawassi M,Mietkiewska E,Gofman R,et al.Unusual sequence relationship between two isolates of citrus tristeza virus[J].J Gen Vinlo,1996,77:2395-2364
[106]Yang Z N,Mathews D H,Dodds J A,et al.Molecular characterization of isolate of citrus tuisteza virus that cause severe symptoms in sweet orange[J].Virus Genes,1999,19:131-142
[107]Vives M C,Rubio L,Lopez C,et al.The complete gonome sequence of the major component of a mild citrus tristeza virus isolate[J].J Gen Virol,1999,80:811-816
[108]Karasev A V,Dawson W O,Hilf M E,et al.Molecular biology of citrus tristeza virus:implications fordisease diagnosis and control[J].Acta Horticulturuae,1998,472:333-337
[109]Whiteside J O,Timmer L W.Compendium of citrus diseases[M].St.Paul:American Phytopathology Society Press,1988:48-50
[110]陈杰忠,陈全友,吴云伦.不同柑橘品种衰退病—茎陷点症状调查[J].中国柑橘,1992,21(2):26-28
[111]Bar-Joseph M,Lee R F.The continuous challenge of citrus tristeza virus control[J].Annual Review Phytopathology,1989,27:291-316
[112]Broadbent P.Biological characterization of Australian isolates of citrus tristeza virus and separation of subisolates by single aphid transmissions[J].Plant Disease,1996,80(3):329-333
[113]Fagoaga C,Semancik J S,Duran-Vila N.A citrus exocortis viroid variant from broad bean(Vicia faba L.):infectivity and pathogenesis[J].Gen Virol.1995,76(9):2271-2277
[114]Garcia-Arenal F,Pallas V,Flores R.The sepuence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid.[J].Nucleic Acids Res.1987,26(15):4203-4210
[115]Visvader J E,Symons R H.Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity[J].Nucleic Acids Res,985,13(8):2907-2920
[116]Mishra M D,Hammond R W,Owens R A,Smith D R,Diener T 0.Indian vunchy top disease of tomato plants is caused by a distinct strain of citrus exocortis viroid.[J].Gen Virol.1991,72:1781-1785
[117]Takao Ito,Hiroyuki Ieki,Katsumi Ozaki.Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction[J].Journal of Virological Methods,2002,106:235-239
[118]Yoshikawa N,Imazumi M,Takabashi T,et al.Striking similarities between the nucleotide sequence and genome organization of citrus tatter leaf and apple stem grooving capilloviruses[J].G Virol,1993,74:2743-2747
[119]Ohira K,Namba S,Rozanov M.Complete sequence of an infectious full-length cDNA clone of citrus tatter leaf capillovirus:comparative seqrence analysis of capillovirus genomes,[J].G Virol,1995,76:2305-2309
[120]Magome H,Yoshekawa N,Takahashi T.Molecular variability of the genomes of capilloviruses from apple,Japanses pear,European pear,and citrus trees[J].Phytopathol,1996,87:389-396
[121]Nishio T,Kawai A,Takahashi T.Purification and propeties of citrus tatter leaf virus.Ann Phytopath Soc Fpn,1989,55:254-258
[122]Inouye N,Maeda T,Mitsuhata K.Citrus tatter leaf virus isolated from lily[J].Ann Phytopathol Soc Jpn,1979,45:712-720
[123]Semancik JS,Weathers LG Partial purification of a mechanically transmissbile virus associated vith tatter leaf of citrus[J].Phytopathol,1965,55:1354-1357
[124]王健华.海南省CMV黄灯笼辣椒分离物的亚组鉴定[D].海南儋州:华南热带农业大学,2004
[125]施曼玲,周雪平.植物病毒的诊断技术[J].微生物学通报,2000,27(2):149-151
[126]Spiegel S,Scott S W,Bowman Vance V,et al.Improved detection of Primus necrotic ringspot virus by the polymerase chain reaction[J].European Journal of Plant Pathology,1996,102(7):681-685
[127]Kinard G R,Scott S W,Barnett 0 W,Detection of apple chlorotic leaf spot and apple stem grooving viruses using RT-PCR[J].Plant Disease,1996,80(6):616-621
[128]张仲凯,李毅.云南植物病毒[M].北京:科学出版社,2001
[129]除为平,谢联辉.黄瓜花叶病毒亚组研究进展[J].福建农业大学学报,1998,27(1):82-91
[130]Hatta T,Francki R L.Cytopathic structures associated with tonoplasts of plant cells infected with cucumber mosaic and tomato aspermy virus[J].Journal of General Virology,1981,53:343-347
[131]陈集双,周雪平,洪健.九种Potyviruses柱状内含体结构的比较研究[J].电子显微学报,1995(1):39-46
[132]Derrick K S.Quantitative assay for plant viruses using serologically specific electronmicroscopy[J].Virology,1973,56:652-657
[133]Louro D,Lesemann D E.Use of proteinA-gold complex for specific labeling of antibodiesbound to plant viruses-Viral antigens in suspensions[J].Journal of Virological Methods,1984,9:107-122
[134]袁小环,李青.血青学方法和分子生物学方法检测植物病毒研究进展[J].热带农业科学,2001,21(6):63-68
[135]孙伟,焦奎.酶联免疫吸附分析法在植物病毒检测中的应用[J].化学研究与应用,2002,14(5):511-514
[136]卢圣栋.现代分子生物学实验技术[M].第2版,北京:中国协和大学出版社,1999,195-220
[137]王明霞,陈菁瑛.两种非放射性DNA探针检测菊花矮化类病毒的比较[J].福建省农科院学报,1997,12(1):56-59
[138]Dietzgen R G,Xu Z,et al.Digoxigenin-labeled cRNA probe for the detection of two potyviruses infecting Peanut[J].Plant Disease,1994,78:708-711
[139]Harber S,Wakarchuk D A,et al.Diagnosis of flame chlorosis,A viruslike disease of Cereals by detection of disease specific RNA with Digoxigenin-labeled RNA[J].Plant Disease,1992,76:590-594
[140]Harper K,Creamer R,et al.Hybridization detection of insert transmitted plant virus with Digoxigenin-labeled probes[J].Plant Disease,1995,79:563-567
[141]邓晓东,刘志等.地高辛标记的DNA探针和ID-ELISA检测芜菁花叶病毒的比 较[J].热带作物学报,1997,18:48-52
[142]朱水芳,相宁.PCR和DIG-cRNA探针检测番茄环斑病毒[J].植物检疫,1995(4):29-31
[143]邱文平.植物病毒分子生物学检测方法[J].热带作物研究,1992(2):65-69
[144]于绍夫.落叶果树的主要病毒病害[M].北京农业出版社,1993,80
[145]牛建新,李东栋.葡萄病毒病的双链RNA(dsRNA)检测技术研究[J].果树学报,2002,19(3):149-152
[146]穆里斯K B,费里F,吉布斯R等.聚合酶链式反应[M].北京科学出版社,1997
[147]Borja M J,Ponz F.An appraisal of different methods for the detection of the walnut strain of cherry leaf roll virus[J].Journal of Virological Methods,1992(36):78-83
[148]Parakh D B,Shamioul AM,Hadidi A,et al.Detection of prune dwarf ilarvirus from infected stone fruits using reverse transcription-polymerase chain reaction[J].Acta HorticμLture,1995(386):421-430
[149]Wetze T,Candresse T.A polymerase chain reaction assay adapted to plum pox potyvirus detection[J].Journal of Virological Methods,1991(33):355-365
[150]Wetzel T,Candresse T.A highly sensitive immunocapture polymerase chain reaction for plum pox potyvirus detection[J].Journal of Virological Methods,1992(39):27-37
[151]孔宝华,陈海如.RT-PCR检测李坏死环斑病毒的研究[J].植物检疫,2000,14(5):257-260
[152]Hyun Ryu K,Yoon K E,Wok P.Detection by RT-PCR of Cymbidium mosaic virus in Orchids[J]Journal of Phytopathology,1995(143):643-646
[153]潘俊松,刘志昕.剑兰花叶病毒外壳蛋白基因序列分析及病毒检测[J].农业生物技术学报,1999,7(4):325-328
[154]吴淑华,王朝辉.江苏省玉米粗缩病病毒原病毒的RT-PCR检测[J].农业生物技术学报,2000,8(4):369-372
[155]闻伟刚,赵秀玲,翁志平.一步RT-PCR检测南芥菜花叶病毒方法的建立[J].植物检疫,2003,17(6):330-332
[156]Rowhani A,Maningas M A,Lile L S,et al.Development of a detection system for viruses of woody plant based on PCR analysis of immobilized virions[J].Phytopathology,1995,83(3):347-352
[157]赵文军,安德荣,朱水芳等.番茄环斑病毒HC-RT-PCR-ELISA检测[J].微生物学报,2003,43(2):174-179
[158]朱建裕,朱水芳,廖晓兰等.实时荧光RT-PCR一步法检测番茄环斑病毒[J].植物病理学报,2003,33(4):338-341
[159]Chamberian J S,Gibbs R A,Ranier J E,et al.Detection Screeninn of the Duchenne Muscular Dystrophy Locus via MultiplexDNA Amplifieation[J].Nuel Acids Res,1988,16:1141-1156
[160]Poncc M R,Robles P,Micol J L.High- throughput genetic mapping in Arabidopsos thaliana[J].Mol Cen Cenet,1999,261:408-415
[161]Rosenfield S I,Javkus L A.A.multiplx reverse transcription polymerase chain reaction method for the detection of foodhorne viruses[J].J Food Prot,1999,62:1210-1214
[162]邹浪萍,杨燕,褚嘉等.多重PCR检测CSFIPO,TPOX和THUI基因座在中国汉族中的多态性[J].遗传学报,1998,25(3):199-204
[163]Rowhani A.,Maningas M.A.,L.S.Development of a detection system for viruses of woody plants based on PCR analysis of immobilized virions[J].Phytopatholo- gy,1995,85:347-351
[164]Mathews D.M.,Riley K.,Dodds J.A.Comparison of detection methods for Citrus tristeza virus in field trees during months of Nonoptimal Titer.[J].Plant Disease,1997,81(5):525-529
[165]Takao Ito,Hiroyuki Ieki,Katsumi Ozaki.Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction[J].Journal of Virological Methods,2002,106:235-239
[166]Sandrine Jagonix,Joseph Marie Bove,Monique Garnier.The phloem-limited bacterium of greening disease of citrus is a member of the alpha subdivision of the proteo-bacteria.[J].International Journal of systematic bacteriology,1994,44:379-386
[167]田亚南,柯穗,柯冲.应用多聚酶链式反应(PCR)技术检测和定量分析柑橘黄龙病病原[J].植物病理学报,1996,26(3)243-250
[168]王中康,孙宪晌,夏玉先等.柑橘溃疡病菌PCR快速检验检疫技术研究[J].植物病理学报,2004,34(1):14-20
[169]袁青,王中康,殷幼平,夏玉先.三重RT-PCR同步检测马铃薯多种病毒影响因素[J].微生物通报,2004,31(5):1-4
[170]吴乃虎.基因工程原理第2版[M].北京科学出版社,2000,100
[171]梁国栋.最新分子生物学实验技术[M].北京科学出版社,2001,129
[172]Rowhani A,Maningas M A,Lile L S,et al.Development of a detection system for viruses of woody plant based on PCR analysis of immobilized virions.[J].Phytopathology,1995,83(3):347-352
[173]陈建军,刘崇怀,古勤生等.DAS-ELISA、RT-PCR和IC-RT-PCR检测葡萄卷叶病毒Ⅲ的比较研究[J].果树学报,2003,20(3):173-177
[174]王继华,瞿素萍.百合无症病毒的RT-PCR和IC-RT-PCR检测[J].云南农业大学学报,2004,19(2):148-150
[175]黄银花,胡晓湘,徐慰卓等.影响多重PCR扩增效果的因素[J].遗传,2003,25(1):65-68
[176]Xianzhou Nie,Rudra P,Singh.Detection of multiple potato viruses using anoligo(dT)as a common cDNA primer in multiplex RT-PCR.[J]Journal of Virological Methods,2000,86:179-185
[177]扬锋.湖南土壤[M].农业出版社,1989,P9-10
[178]湖南省志编篡委员会.湖南省志第二卷地理志(下)[M].1962,P57-68
[179]沈德绪,王元裕,陈力耕.柑橘遗传育种学[M].科学出版社,1998,11-12
[180]沈德绪,,王元裕,陈力耕.柑橘遗传育种学[M].科学出版社,1998,68-72
[181]廖振坤,张秋明.湖南省柑橘品质鉴定及区划研究[J].福建果树,2006(4)P9-12.
[182]沈德绪,王元裕,陈力耕.柑橘遗传育种学[M].科学出版社,1998:173-175.
[183]尹佟明AFLP Molecular Markers and Application in Plant Breeding[J]生物工作进展,1997,17(1):6-11.
[184]祝军,王涛,赵玉军等.Identification of Apple Varieties with AFLP Molecular Markers[J].园艺学报,2000,27(2):102-106.
[185]潘新法,孟祥勋,曹广力等.Application of RAPD-PCR technique for Identification of Varieties in Loqum[J].果树学报,2002,19(2):136-138.
[186]鲍露,徐昌杰,江文彬等.Establishment of AFLP protocol for grape and cultivar distinquishing of chaoteng and Fujinori[J].果树学报,2005,22(4):422-425.
[187]郑学勤,李嘉瑞,吴燕民等.Identification of Apricot(Ameniaca spp.)Using RAPD Analysis[J].园艺学报,2000,27(1):55-56.
[188]刘成明,梅曼彤.Identification of Stenospermocarpy Mutants of Litichi by RAPD Analysis[J].园艺学报,2002,29(1):57-59.
[189]易干军,于晓英,霍合强等.Identification and Classification of Banana(Musa AAA Group Cavendish)V arieties byAFLP[J].果树学报,2002,19(4):247-251.
[190]陈晓流,陈学森,束怀瑞等.RAPD Analysis of 15 Cherry Cultivars[J].果树学报,2004,21(6):556-559.
[191]谭晓风,胡芳名,张党权等.Molecular Identification of Main Cultivars of Torreya grandis by RAPD Markers[J].园艺学报,2002,29(1):69-71.
[192]宁允叶,熊庆蛾,曾伟光等.Studies on Red Flesh Sport from‘Red Sun'Kiwifruit Using RAPD Marker[J].园艺学报,2003,30(5):511-513.
[193]乔玉山,章镇,房经贵.Optical Reaction System of RAPD in Chinese Plum and Its Preliminary Use for Cultivar Identification[J]果树学报,2003,20(6):445-449.
[194]马兵钢,牛建新,潘立忠.Study on the Genetic Relationships Among 18 Pear Cultivars by RAPD[J]果树学报,2004,21(6)521-525.
[195]房经贵,章镇,周兰华.The Possibility of Using RAPD Marker for the Identification of Fruit Sport[J].果树学报,2001,18(3):182-183.
[196]乔飞,王力荣,范崇辉等.利用AFLP和RAPD标记构建桃的遗传连锁图[J].果树学报,2006,23(5):766-769.
[197]吴子龙,方连玉,王军等.15份葡萄种质亲缘关系的ISSR分析[J].果树学部委员报,2006,23(4):638-641
[198]徐崇志,廖胜刚.分子标记技术在果树种质资源及遗传育种研究中的应用[J].塔里木大学学报,2006,vol.18 No23:39-44
[199]文晓鹏.厐晓明,邓秀新.刺梨及部分近缘种形态学性状和RAPD分析[J].园艺学报,2003,30(2):204-206
[200]扬亚洲,王跃进,张剑侠等.中国葡萄属野生种抗旱基因的分子标记及遗传分析[J].园艺学报,2007,34(5):1087-1092
[201]吴锦程,杨向辉,林顺权等.枇杷AFLP分析体系的建立与应用[J].果树学报,2006,23(5):774-778.
[202]王斐,林盛华,方成泉等.梨新品种及其亲本的AFLP分析[J].园艺学报,2007,34(4):847-852
[203]江东,李喜庆,熊光明等.DNA Extraction Method for AFLP Analysis in Citrus[J]果树学报)2002,19(4)267-268.
[204]Moller EM,Bahnweg G,A simpleand efficient protocol for isolation of high molecular weight DNA from fiamentous fungi,fruit bodies,and infected plant tissues[J].Nucleic Acids Research,1992,20(22):6115-6116.
[205]GREEN,R.M.,VARDI,A.and E.GALUN.The plastome of Citrus physical map,Variation among Citrus cultivars and species and comparison with related genera thoer[m].Appl.genet.72:170-177
[206]SWINGLE,W.T.and P.C.REECE.The Botany of Citrus and Its Wild Relatives.[m]Citrus Industry Vol.Ⅰ 1967:279-381
[207]He.S.W,Liu,G R,and Xiang.D.M.On Three Problems in The Study of Germplasm Resources of Citrus[m].Proc Int Soc.Citriculture,Vol Ⅰ 1984:100-103,
[208]刘庚峰,贺善文,李文斌.莽山野橘.我国南岭山脉又一柑橘野生新种[J].湖南园艺,1987:1-4
[209]刘庚峰,贺善文,李文斌.中国真正柑橘亚属两野生新种[C].全国果树种质资源学术讨论会中国园艺学会,1987:43-45
[210]李文斌,贺善文,刘庚峰.湖南野生柑橘类种质过氧化物酶同功酶分析[J].园艺学报1987,14(3):151-160
[211]陈梦龙.湖南几种野生宽皮柑橘的亲缘关系研究.植物学性状调查与花粉粒形态观察[C].全国果树种质资源学术讨论会.中国园艺学会,1987:26-29
[212]易干军,谭卫萍,霍合强.龙眼品种种(系)遗传多样性及亲缘关系的AFLP分析[J].园艺学报,2003,30(3):272-276
[213]易干军,霍合强,陈大成,等.荔枝品种亲缘关系的AFLP分析[J].园艺学报,2003,30(4):399-403
[214]田亚南,柯穗,李韬等.应用电镜与PCR技术检测珀溪蜜袖黄龙病病原[J].植物病理学报,2000,30(1):76-81.
[215]李农一,唐庚元,毛晓林.柑橘木虱在湖南省的分布[J].植物检疫,1992(6)5:326-328.
[2]叶荫民.柑橘品种改良的世纪回顾与展望[J]中国南方果树.2000,29(3):15-17
[3]伊华林.美国柑橘遗传资源收集保存和和评价体系[J].中国南方果树,2003,32(4):16-20
[4]刘新录,王小兵,邓秀新.巴西柑橘考察报告[J].中国南方果树,2003,32(5):20-26
[5]陈竹生.我国柑橘种质保存评价利用现状分析[J].中国南方果树,2000,29(1):14-15
[6]湖南省园艺研究所.湖南野生柑橘资源的初步调查简报[C].湖南园艺科技资料果树专辑(1975-1976),1976,P.8-10
[7]贺善文.柑橘类种质资源中心问题的初步探讨[J].园艺学报,1978,6(1):19-25
[8]李文斌,贺善文,刘庚峰.湖南野生种类种质过氧化物酶同工酶分析[J].园艺学报,1987,14(3):151-160
[9]Swingle.W.T.and Teece,P.C.The botany and citrus and its wild relatives[M].The Citrus Industry,1967,Vol.1:190-430.Berkeley:Division of Agricultural Sciences,University of California.
[10]Barrett.H.C.Intergeneric hybridization of citrus and other genera in citrus variety improvement.[J].Proc.Int,Soc.Citriculture 1977,PP.586-9.LakeAlfred,FL:ISC.
[11]Hodgson,R.W.Taxonomy and nomenclature in Citrus[J].Int.Org.Citrus Virol.Proc,1961,2:1-7
[12]贺善文.中国柑橘分类苦干问题[J].中国果树,1962,(2):28-30
[13]曾勉.对柑橘分类的认识体系和整理意见[J].中国果树,960,(2):31-37
[14]蒋聪强.论柑橘三属左右线分类系统[J].西南农学院学报,1983,(4):10-23
[15]林正奎.柑橘属11个类群叶精油成分与系统进化关系[J].植物学报,1992,34(2):133-139
[16]H.C.Dass,et al.Phylogenetic studies in Citrus reticulata and related type.[J].Euphytica,1977,26:805-810
[17]H.C.Dass,et al,Leaf flavonoid Patterns of some important citrus speeies.[J].Ind.J.Exp.Bilo,1978,16:62-65
[18]A.Esen et al.Distribution of enzymatic browning of young shoot homogenates in the Aurantinoideae.[J].Amer.J.Bot.,1975,62(10):1078-1803
[19]A.Esen et al.A simple and rapid screening procedure for identification of zygotic citrus seedlings among crosses of certain taxa[J].Amer.Soc.Hort.Sci.,1975,100(5):558-561
[20]A.Esen et al.Seperation of nucellar and zygotic citrus seedlings by use of polyphenoloxidase catalyzed browning[J].Proc.Int.Soc.Citriculture,1977,2:616-618
[21]朱立武.中国柑橘数量化学分类研究.植物分类学报,1988,26(5):353-361
[22]A.M.Torrse,et al.Leaf isozymes as genetic markers in citrus[J].Amer.J.Bot.,1978,65:869-881
[23]R.K.Soost,et al.Leat isozymes as genetic markers in citrus[J].Proc.Int.Soc.Citriculture,1981,1:7-10
[24]吴安仁.用过氧化物同工酶对柑橘分类的探讨[J].园艺学报,1985,12(2):83-87
[25]李文斌,贺善文.湖南野生柑橘类种质过氧化物酶同工酶的分析[J].园艺学报,1987.14(3):153-160
[26]廖映玢.柑橘近缘植物酯酶同工酶的研究[J].植物学报,1988,30(2):163-168
[27]钟广炎,叶荫民.柑橘过氧化物酶同工酶的遗传规律[J].中国柑橘,1989,19(3):22-24
[28]方德秋,章文才.我国果树同工酶研究现状及展望[J].福建果树,1989,(4):16-20
[29]方德秋,章文才.同工酶研究在柑橘上的应用[J].湖北农业科学,1990,(7):23-24
[30]方德秋,章文才.应用同工酶进行宽皮柑橘分类及其进化研究[J].武汉植物学研究,1992,10(4):305-312
[31]方德秋,章文才.柑橘分类研究的新方法[J].果树科学,1993,10(增刊):29-33
[32]叶荫民,孙炎,郑向红.柑橘花粉形态研究[J].中国农业科学,1982,(5):62-65
[33]熊兴耀,魏文娜,李润唐.柑橘染色体组型及Giemsa带型的研究初报[J].湖南农学院学报,1982,(2):61
[34]梁国鲁.柑橘类的细胞分类学研究[J].武汉植物学研究,1990,8(1):1-7
[35]梁国鲁,殷智勇.宽皮柑橘两个分类群的核型及C带带型分析[J].西南农大学报,1992,14(4):320-322
[36]陈喜文,陈梦龙.湖南柑橘资源的植物血清学研究[J].湖南农学院学报,1991,17(4):714-719
[37]尹佟明,黄敏仁.AFLP分子标记及其在育种上的应用[J].生物工程进展,1997,17(1):6-11
[38]吴俊,魏钦平.分子标记及其在果树种质资源研究中的应用[J].安徽农业大学学报,2002,29(2):158-162
[39]Williams,J.G.K.,Kubelik A.R.,Livak K.J.,Rafalski J.A.& TingeyS.V.,O.DNA polymorphisms amplified by arbitrary primers as useful as genetic markers.[J].Nucleic Acid Res,1990,18:6531-6535
[40]Paran,I.and R.W.Michelmore.Development of reliablePCR-based markers linked to downy mildew resistance genes inlettuce.[J].Theor.Appl.Gen.1993,85:985-993
[41]Jung,G.,P.W.Skroch,J.Nienhuis,D.P.Coyne,E.Arnaud-Santana,H.M.Ariyarathne,and J.M.Marita.Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean[J].Crop Sci.1999,39:1448-1455
[42]Hernandez,P.,A.Martin,and G.Dorado,Development of SCARs by direct sequencing of RAPD products:a practical tool for the introgression and marker-assisted selection of wheat[J].Mol.Breed.1999,5:245-253
[43]Vos.P,Hogers R,Blecker M,etal.AFLP:a new technique for DNA fingerprinting[J].Nucl Acids Res,1995,23(21):4407-4414
[44]Cho YG,Blair MW,Panaud O,et al.cloning and mapping of variety specific rice genomic DNA sequences:Amplified Fragment Length Polymorphism(AFLP)from silver-stained polyacrylamide gels.[J].Genome,1996,39:373-378
[45]Donini P,Elles ML,Bongound SM,et al.(AFLP)fingerprinting reveals pattewn differences between template DNA extracted from different plant organs[J].Genome,1997,40:521-526
[46]Stella M,Resmon-Buttner JS,Christian J,et al.AFLP Markers tightly linked to the sex locus in Asparagus officinalis[J].Molecular Breeding,1998,4:91-98
[47]Beeker J,Vos P,Kupr M,et al.Combined mapping of AFLP and RFLP markers in barley.[J].Mol.Gen Genet,1995,249:65-73
[48]Maria Teresa Cervera,Narijke Steenackers,et al.AFLP molecular markers for resistance to melamp soralarici-populina in poplar.Environmental and social issues in Poplar and Willow cultivation and utilization,1996,Vol 1,142-150
[49]Cervera M T,et al.Identification of AFLP molecular markers for resistance against Melampsora larici-populina in populus[J].Theor Appl Genet,1996,9.(5-6):733-737
[50]Hyman BC,et al.Application of mitochondrial DNA polymorphism to Meloidogyne molecular population biology[J].Nematology,1996,28(3):268-276
[51]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20:176-183
[52]Yamamoto M.et al.Polygenic relationships of Citrus reveaded by RFLP analysis of mitochon drial and chloroplast DNA[J].Japan J.Breed.1993,43:355-365
[53]熊光明,梁国鲁,吴清.适于AFLP分析用的柑橘DNA提取方法[J].果树学报,2002,19(4):247-251
[54]范眸天,高俊,吴兴恩.十五种柑橘种质资源的RAPD分析[J].中国南方果树,2002:
[55]Ros A C.Maya MM,Duque M C,et al.AFLP analysis of relationships among cassava and other Manihot species[J]Theoretical and Applied Genetics,1997,95(5-6):741-750
[56]庞晓明,胡春根,邓秀新.AFLP标记在果树遗传研究中的应用[J].生命的化学,2001,21卷第5期
[57]Aggarwal RK,Brar DS,Nandi S,Huang N,Khush GS.Phylogenetic relationships among Oryza species revealed by AFLP markers.[J].Theor Appl Genet,1999,98:1320-1328
[58]Sharma SK.Knox MR.Ellis THN.AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis[J].Theor Appl Genet.1996,93:75
[59]ZabearM,Vos P.Selective restriction fragment amplification,ageneral method for DNA finger printings[J]European Patent Applification,Publication No:858Al,1993.
[60]Vos.P,Hogers R,Blecker M,etal.AFLP:a new technique for DNA fingerpri nting[J].Nucl Acids Res,1995,23(21):4407-4414
[61]徐红梅,秦岭.AFLP技术及其在果树研究中的应用进展[J].北京农学院学报,2003,18(4):308-310
[62]Lin Jhy-jhu,et al.Identification of molecular markers in soybean comparing RFLRRAPD and AFLP DNA mapping techniques[J].Plant Molecular Biology Reporter,1996,14(2):156-169
[63]Mibourne E,et al.Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato[J].Molecular Breeding,1997,3(2):127-136
[64]International plant genome conference Ⅲ January,1995,SanDicgo,USA
[65]陶文静,刘大钧.AFLP技术及其在植物基因组研究中的应用[J].世界农业,1998,12(总57-236)1-758
[66]陈秀蕙.菠萝DNA导入黄瓜的初步研究[J].海南大学学报自然科学版,1998,16(1):62-68
[67]M.F.DuvalJ.L.Noyer · X.PerrierG Coppens d'Eeckenbrugge · P.Hamon.Molecular diversity in pineapple assessed by RFLP markers[J].Theor Appl Genet(2001)102:83-90
[68]Claudete de Fatima Ruas,Paulo Maurlcio Ruas.Assessment of genetic relatedness of the genera Ananas and Pseudananas confirmed by RAPD markers[J].Euphytica 2001.119:245-252
[69]易干军,于晓英,霍合强等.应用AFLP进行香牙蕉品种(系)的鉴别与分类[J].果树学报,2002,19(4):247-251
[70]Lu ZX,et al.Construction of a gentic linkage map and identification of AFLP marke rs for resistance to root-knot nematodes in peach rootstocks[J].Gemome,1998,41(2):199-207
[71]Harvey C.F,Gill GP,Fraser L.G and Mc Neilage M.A.Sex determination in Actinidia.sex-linked markers and progeny sex ratio in diploid.A chinensis[J].Sex Plant Report,1997,10:149-154
[72]张潞生,李传友.猕猴桃雌雄性别的AFLP标记初报[C].武汉:1998国际猕猴桃研讨会论文集,1998,17-18
[73]Testolin R,Huang W-G,Cipriani G.Towards a linkage map in kiwifruit based on microsatellites and saterated with AFLP markers[J].Acta Hort,1999,48/98:79-84
[74]TRAVIS.SE,MASCHINIKIJ,LEIM P.An analysis of biological variation in Asstragalus crem nophylax var.crem nophylax,a critically endangered plant.using AFLP markers[J].Mol Ecol,1996,5 6:735-745
[75]章一华.植物遗传资源研究的一个新领域-核心样品[J].作物品种资源,1993(2):1-3.
[76]LEFEBVRF V,GOFFINET B.CHAUVET JC,etal.Evaluation of Genetic distances between pepper inbred lines for cultivar protection purposes:comparison of AFLP,RAPD and phenotypic data[J].The or ApplGenet,2001,102:741-750
[77]房经贵,马正强.AFLP标记在两个芒果品种间杂交F1代的多态性及分离方式[J].中国农业科学,2000,33(3):19-24
[78]Waugh R,Bonar N,etal.Homology of AFLP products in three mapping populations of barley.[J].Mol Gen Genet,1997,255:311-321
[79]Sosinki B,Sossey-Alaoui K,etal.Use of AFLP and RFLP markers to create a combined linkage map in peach for use in marker assisted selection[J].Acta Hort,1998,465:61-68
[80]张潞生,李传友,贾建航.猕猴桃雌雄性别的AFLP鉴别中DNA模板的制备[J].果树科学,1999,16(3):171-175
[81]祝军,王涛,赵玉军.应用AFLP分子标记鉴定苹果品种[J].园艺学报,2000,27(2):102-106
[82]庞晓明,邓秀新,胡春根.枳属36份特异种质的AFLP指纹图谱构建与分析[J].园艺学报,2003,30(4):394-398
[83]史永忠,郭文武,邓秀新.柑橘RAPD技术体系建立与体细胞杂种鉴定[J].园艺学报,1998,25(2):105-110
[84]郝玉金.柑橘和平果等果树种质资源的离体保存及遗传变异[D],华中农业大学,1999:33-61
[85]CardinerSE,BassettHCM,MADie C.Isozyme,randomLy amplified polymorphic DNA and restriction fragment length polymorphism markers used to deduce aputative panent or the brabure apple[J].Amer SocHort Sci,1996,121(6):996-1001
[86]王丙旭.RAPD在梨种质资源亲缘关系和品种鉴定中的应用.[D],长春,吉林农业大学,1998
[87]柯穗,唐伟文,高乔婉.柑橘黄龙病病原物提纯方法和血清学的初步研究[J].华南农业大学学报,1989,10(1):1-8.
[88]Gamier M,MartinGros G,Bove J M.Monoclonal antibodiesagainst thebacterial-like organism associated with citrus greening disease[J].Ann Microbiol,1987,(138):639-650.
[89]Gamier M,Gao S J,He YL,et al.Study of the greening organism(GO)with monoclonal antibodies:serological identification,morphology,serotypes and purification of the GO[A].Proc 11~(th)Conf IOCV[C].Riverside IOCV,1991,428-435.
[90]Villechanoux S,Gamier M,Laigret F,etal.Detection of several strains of the bacteria-like organism of citrus greening disease by DNA probes[J]Curr Microbiol,1992,(24):89-95.
[91]Villechanoux S,The genome of the non-cultured,bactetia21ike organism of citrus associated with citrus greening disease contains the nusGrplKAJLrpoBC gene cluster and gene for a bacteriophage type DNA polymerase[J].Curr Microbiol,1993,(26):161:166.
[92]Jagoueix S,Bove J M,Gamier M.The phloem-limited bacterium of greening disease of citrus is a member of thea-subdivision of the proteobacteria[J].Int J Syst Bacteriol,1994,(44):379-386.
[93]张天淼.柑橘病毒病[M].北京中国农业出版社,2000.
[94]Kapur,S.P.,Ananda,S.A.,Cheema,S.S.,Kapoor,S.K.1977.Prevalence of greening marker substances in different species of citrus.[J].Indian J.Horticult.34:205-8(Rev.Plant Pathol.60:54)
[95]王运生,戴良英,荒井答.日本柑橘黄龙病病原体电镜观察及PCR检测[J].湖南农业大学学报(自然科学版),2004,10,446-449.
[96]田亚南,柯穗,李韬等.应用电镜与PCR技术检测琯溪蜜袖黄龙病病原[J].植物病理学报.2000,30(1):76-81.
[97]田亚南,柯穗,柯冲.柑橘黄龙病诊断法的研究进展[J].福建农业学报,1998,13(1):27-35.
[98]吴世盘.柑橘黄龙病的直接荧光诊断法[J].华南农业大学学报,1987,8(2):45-48.
[99]林尤剑,高日霞.应用FBA染色法观察柑橘黄龙病病株中的病原难培养细菌[J].福建农学院学报,1990,19(1):1-6.
[100]柯穗,唐伟文,高乔婉等.柑橘黄龙病病原物提纯方法和血清学的初步研究[J].华南农业大学学报,1989,10(1):1-7.
[101]Bar-Joseph M,Roistacher C N,Garnsey S M,et al.A review on tristeza,an on going threat to citriculture[J].Proc,Int Soc Citriculture,1981,1:419-423
[102]张作芳,张成良,相宁等.禁止和限制入境植物病毒的研25-柑橘速衰病毒[J]. 植物检疫研究报告,1990:1-27
[103]Karasev A V,Boyko V P,Gowda S,et al.Conplete sequence of the citrus tristezavirus RNA genome[J].Virology,1995,208:511-520
[104]Pappu H R,Karasev A V,Anderson E J,et al.Nucleotide sequence and organization of 3' open reading frames of the citrus tristeza virus genome[J].Virology,1994,99:35-46
[105]Mawassi M,Mietkiewska E,Gofman R,et al.Unusual sequence relationship between two isolates of citrus tristeza virus[J].J Gen Vinlo,1996,77:2395-2364
[106]Yang Z N,Mathews D H,Dodds J A,et al.Molecular characterization of isolate of citrus tuisteza virus that cause severe symptoms in sweet orange[J].Virus Genes,1999,19:131-142
[107]Vives M C,Rubio L,Lopez C,et al.The complete gonome sequence of the major component of a mild citrus tristeza virus isolate[J].J Gen Virol,1999,80:811-816
[108]Karasev A V,Dawson W O,Hilf M E,et al.Molecular biology of citrus tristeza virus:implications fordisease diagnosis and control[J].Acta Horticulturuae,1998,472:333-337
[109]Whiteside J O,Timmer L W.Compendium of citrus diseases[M].St.Paul:American Phytopathology Society Press,1988:48-50
[110]陈杰忠,陈全友,吴云伦.不同柑橘品种衰退病—茎陷点症状调查[J].中国柑橘,1992,21(2):26-28
[111]Bar-Joseph M,Lee R F.The continuous challenge of citrus tristeza virus control[J].Annual Review Phytopathology,1989,27:291-316
[112]Broadbent P.Biological characterization of Australian isolates of citrus tristeza virus and separation of subisolates by single aphid transmissions[J].Plant Disease,1996,80(3):329-333
[113]Fagoaga C,Semancik J S,Duran-Vila N.A citrus exocortis viroid variant from broad bean(Vicia faba L.):infectivity and pathogenesis[J].Gen Virol.1995,76(9):2271-2277
[114]Garcia-Arenal F,Pallas V,Flores R.The sepuence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid.[J].Nucleic Acids Res.1987,26(15):4203-4210
[115]Visvader J E,Symons R H.Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity[J].Nucleic Acids Res,985,13(8):2907-2920
[116]Mishra M D,Hammond R W,Owens R A,Smith D R,Diener T 0.Indian vunchy top disease of tomato plants is caused by a distinct strain of citrus exocortis viroid.[J].Gen Virol.1991,72:1781-1785
[117]Takao Ito,Hiroyuki Ieki,Katsumi Ozaki.Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction[J].Journal of Virological Methods,2002,106:235-239
[118]Yoshikawa N,Imazumi M,Takabashi T,et al.Striking similarities between the nucleotide sequence and genome organization of citrus tatter leaf and apple stem grooving capilloviruses[J].G Virol,1993,74:2743-2747
[119]Ohira K,Namba S,Rozanov M.Complete sequence of an infectious full-length cDNA clone of citrus tatter leaf capillovirus:comparative seqrence analysis of capillovirus genomes,[J].G Virol,1995,76:2305-2309
[120]Magome H,Yoshekawa N,Takahashi T.Molecular variability of the genomes of capilloviruses from apple,Japanses pear,European pear,and citrus trees[J].Phytopathol,1996,87:389-396
[121]Nishio T,Kawai A,Takahashi T.Purification and propeties of citrus tatter leaf virus.Ann Phytopath Soc Fpn,1989,55:254-258
[122]Inouye N,Maeda T,Mitsuhata K.Citrus tatter leaf virus isolated from lily[J].Ann Phytopathol Soc Jpn,1979,45:712-720
[123]Semancik JS,Weathers LG Partial purification of a mechanically transmissbile virus associated vith tatter leaf of citrus[J].Phytopathol,1965,55:1354-1357
[124]王健华.海南省CMV黄灯笼辣椒分离物的亚组鉴定[D].海南儋州:华南热带农业大学,2004
[125]施曼玲,周雪平.植物病毒的诊断技术[J].微生物学通报,2000,27(2):149-151
[126]Spiegel S,Scott S W,Bowman Vance V,et al.Improved detection of Primus necrotic ringspot virus by the polymerase chain reaction[J].European Journal of Plant Pathology,1996,102(7):681-685
[127]Kinard G R,Scott S W,Barnett 0 W,Detection of apple chlorotic leaf spot and apple stem grooving viruses using RT-PCR[J].Plant Disease,1996,80(6):616-621
[128]张仲凯,李毅.云南植物病毒[M].北京:科学出版社,2001
[129]除为平,谢联辉.黄瓜花叶病毒亚组研究进展[J].福建农业大学学报,1998,27(1):82-91
[130]Hatta T,Francki R L.Cytopathic structures associated with tonoplasts of plant cells infected with cucumber mosaic and tomato aspermy virus[J].Journal of General Virology,1981,53:343-347
[131]陈集双,周雪平,洪健.九种Potyviruses柱状内含体结构的比较研究[J].电子显微学报,1995(1):39-46
[132]Derrick K S.Quantitative assay for plant viruses using serologically specific electronmicroscopy[J].Virology,1973,56:652-657
[133]Louro D,Lesemann D E.Use of proteinA-gold complex for specific labeling of antibodiesbound to plant viruses-Viral antigens in suspensions[J].Journal of Virological Methods,1984,9:107-122
[134]袁小环,李青.血青学方法和分子生物学方法检测植物病毒研究进展[J].热带农业科学,2001,21(6):63-68
[135]孙伟,焦奎.酶联免疫吸附分析法在植物病毒检测中的应用[J].化学研究与应用,2002,14(5):511-514
[136]卢圣栋.现代分子生物学实验技术[M].第2版,北京:中国协和大学出版社,1999,195-220
[137]王明霞,陈菁瑛.两种非放射性DNA探针检测菊花矮化类病毒的比较[J].福建省农科院学报,1997,12(1):56-59
[138]Dietzgen R G,Xu Z,et al.Digoxigenin-labeled cRNA probe for the detection of two potyviruses infecting Peanut[J].Plant Disease,1994,78:708-711
[139]Harber S,Wakarchuk D A,et al.Diagnosis of flame chlorosis,A viruslike disease of Cereals by detection of disease specific RNA with Digoxigenin-labeled RNA[J].Plant Disease,1992,76:590-594
[140]Harper K,Creamer R,et al.Hybridization detection of insert transmitted plant virus with Digoxigenin-labeled probes[J].Plant Disease,1995,79:563-567
[141]邓晓东,刘志等.地高辛标记的DNA探针和ID-ELISA检测芜菁花叶病毒的比 较[J].热带作物学报,1997,18:48-52
[142]朱水芳,相宁.PCR和DIG-cRNA探针检测番茄环斑病毒[J].植物检疫,1995(4):29-31
[143]邱文平.植物病毒分子生物学检测方法[J].热带作物研究,1992(2):65-69
[144]于绍夫.落叶果树的主要病毒病害[M].北京农业出版社,1993,80
[145]牛建新,李东栋.葡萄病毒病的双链RNA(dsRNA)检测技术研究[J].果树学报,2002,19(3):149-152
[146]穆里斯K B,费里F,吉布斯R等.聚合酶链式反应[M].北京科学出版社,1997
[147]Borja M J,Ponz F.An appraisal of different methods for the detection of the walnut strain of cherry leaf roll virus[J].Journal of Virological Methods,1992(36):78-83
[148]Parakh D B,Shamioul AM,Hadidi A,et al.Detection of prune dwarf ilarvirus from infected stone fruits using reverse transcription-polymerase chain reaction[J].Acta HorticμLture,1995(386):421-430
[149]Wetze T,Candresse T.A polymerase chain reaction assay adapted to plum pox potyvirus detection[J].Journal of Virological Methods,1991(33):355-365
[150]Wetzel T,Candresse T.A highly sensitive immunocapture polymerase chain reaction for plum pox potyvirus detection[J].Journal of Virological Methods,1992(39):27-37
[151]孔宝华,陈海如.RT-PCR检测李坏死环斑病毒的研究[J].植物检疫,2000,14(5):257-260
[152]Hyun Ryu K,Yoon K E,Wok P.Detection by RT-PCR of Cymbidium mosaic virus in Orchids[J]Journal of Phytopathology,1995(143):643-646
[153]潘俊松,刘志昕.剑兰花叶病毒外壳蛋白基因序列分析及病毒检测[J].农业生物技术学报,1999,7(4):325-328
[154]吴淑华,王朝辉.江苏省玉米粗缩病病毒原病毒的RT-PCR检测[J].农业生物技术学报,2000,8(4):369-372
[155]闻伟刚,赵秀玲,翁志平.一步RT-PCR检测南芥菜花叶病毒方法的建立[J].植物检疫,2003,17(6):330-332
[156]Rowhani A,Maningas M A,Lile L S,et al.Development of a detection system for viruses of woody plant based on PCR analysis of immobilized virions[J].Phytopathology,1995,83(3):347-352
[157]赵文军,安德荣,朱水芳等.番茄环斑病毒HC-RT-PCR-ELISA检测[J].微生物学报,2003,43(2):174-179
[158]朱建裕,朱水芳,廖晓兰等.实时荧光RT-PCR一步法检测番茄环斑病毒[J].植物病理学报,2003,33(4):338-341
[159]Chamberian J S,Gibbs R A,Ranier J E,et al.Detection Screeninn of the Duchenne Muscular Dystrophy Locus via MultiplexDNA Amplifieation[J].Nuel Acids Res,1988,16:1141-1156
[160]Poncc M R,Robles P,Micol J L.High- throughput genetic mapping in Arabidopsos thaliana[J].Mol Cen Cenet,1999,261:408-415
[161]Rosenfield S I,Javkus L A.A.multiplx reverse transcription polymerase chain reaction method for the detection of foodhorne viruses[J].J Food Prot,1999,62:1210-1214
[162]邹浪萍,杨燕,褚嘉等.多重PCR检测CSFIPO,TPOX和THUI基因座在中国汉族中的多态性[J].遗传学报,1998,25(3):199-204
[163]Rowhani A.,Maningas M.A.,L.S.Development of a detection system for viruses of woody plants based on PCR analysis of immobilized virions[J].Phytopatholo- gy,1995,85:347-351
[164]Mathews D.M.,Riley K.,Dodds J.A.Comparison of detection methods for Citrus tristeza virus in field trees during months of Nonoptimal Titer.[J].Plant Disease,1997,81(5):525-529
[165]Takao Ito,Hiroyuki Ieki,Katsumi Ozaki.Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction[J].Journal of Virological Methods,2002,106:235-239
[166]Sandrine Jagonix,Joseph Marie Bove,Monique Garnier.The phloem-limited bacterium of greening disease of citrus is a member of the alpha subdivision of the proteo-bacteria.[J].International Journal of systematic bacteriology,1994,44:379-386
[167]田亚南,柯穗,柯冲.应用多聚酶链式反应(PCR)技术检测和定量分析柑橘黄龙病病原[J].植物病理学报,1996,26(3)243-250
[168]王中康,孙宪晌,夏玉先等.柑橘溃疡病菌PCR快速检验检疫技术研究[J].植物病理学报,2004,34(1):14-20
[169]袁青,王中康,殷幼平,夏玉先.三重RT-PCR同步检测马铃薯多种病毒影响因素[J].微生物通报,2004,31(5):1-4
[170]吴乃虎.基因工程原理第2版[M].北京科学出版社,2000,100
[171]梁国栋.最新分子生物学实验技术[M].北京科学出版社,2001,129
[172]Rowhani A,Maningas M A,Lile L S,et al.Development of a detection system for viruses of woody plant based on PCR analysis of immobilized virions.[J].Phytopathology,1995,83(3):347-352
[173]陈建军,刘崇怀,古勤生等.DAS-ELISA、RT-PCR和IC-RT-PCR检测葡萄卷叶病毒Ⅲ的比较研究[J].果树学报,2003,20(3):173-177
[174]王继华,瞿素萍.百合无症病毒的RT-PCR和IC-RT-PCR检测[J].云南农业大学学报,2004,19(2):148-150
[175]黄银花,胡晓湘,徐慰卓等.影响多重PCR扩增效果的因素[J].遗传,2003,25(1):65-68
[176]Xianzhou Nie,Rudra P,Singh.Detection of multiple potato viruses using anoligo(dT)as a common cDNA primer in multiplex RT-PCR.[J]Journal of Virological Methods,2000,86:179-185
[177]扬锋.湖南土壤[M].农业出版社,1989,P9-10
[178]湖南省志编篡委员会.湖南省志第二卷地理志(下)[M].1962,P57-68
[179]沈德绪,王元裕,陈力耕.柑橘遗传育种学[M].科学出版社,1998,11-12
[180]沈德绪,,王元裕,陈力耕.柑橘遗传育种学[M].科学出版社,1998,68-72
[181]廖振坤,张秋明.湖南省柑橘品质鉴定及区划研究[J].福建果树,2006(4)P9-12.
[182]沈德绪,王元裕,陈力耕.柑橘遗传育种学[M].科学出版社,1998:173-175.
[183]尹佟明AFLP Molecular Markers and Application in Plant Breeding[J]生物工作进展,1997,17(1):6-11.
[184]祝军,王涛,赵玉军等.Identification of Apple Varieties with AFLP Molecular Markers[J].园艺学报,2000,27(2):102-106.
[185]潘新法,孟祥勋,曹广力等.Application of RAPD-PCR technique for Identification of Varieties in Loqum[J].果树学报,2002,19(2):136-138.
[186]鲍露,徐昌杰,江文彬等.Establishment of AFLP protocol for grape and cultivar distinquishing of chaoteng and Fujinori[J].果树学报,2005,22(4):422-425.
[187]郑学勤,李嘉瑞,吴燕民等.Identification of Apricot(Ameniaca spp.)Using RAPD Analysis[J].园艺学报,2000,27(1):55-56.
[188]刘成明,梅曼彤.Identification of Stenospermocarpy Mutants of Litichi by RAPD Analysis[J].园艺学报,2002,29(1):57-59.
[189]易干军,于晓英,霍合强等.Identification and Classification of Banana(Musa AAA Group Cavendish)V arieties byAFLP[J].果树学报,2002,19(4):247-251.
[190]陈晓流,陈学森,束怀瑞等.RAPD Analysis of 15 Cherry Cultivars[J].果树学报,2004,21(6):556-559.
[191]谭晓风,胡芳名,张党权等.Molecular Identification of Main Cultivars of Torreya grandis by RAPD Markers[J].园艺学报,2002,29(1):69-71.
[192]宁允叶,熊庆蛾,曾伟光等.Studies on Red Flesh Sport from‘Red Sun'Kiwifruit Using RAPD Marker[J].园艺学报,2003,30(5):511-513.
[193]乔玉山,章镇,房经贵.Optical Reaction System of RAPD in Chinese Plum and Its Preliminary Use for Cultivar Identification[J]果树学报,2003,20(6):445-449.
[194]马兵钢,牛建新,潘立忠.Study on the Genetic Relationships Among 18 Pear Cultivars by RAPD[J]果树学报,2004,21(6)521-525.
[195]房经贵,章镇,周兰华.The Possibility of Using RAPD Marker for the Identification of Fruit Sport[J].果树学报,2001,18(3):182-183.
[196]乔飞,王力荣,范崇辉等.利用AFLP和RAPD标记构建桃的遗传连锁图[J].果树学报,2006,23(5):766-769.
[197]吴子龙,方连玉,王军等.15份葡萄种质亲缘关系的ISSR分析[J].果树学部委员报,2006,23(4):638-641
[198]徐崇志,廖胜刚.分子标记技术在果树种质资源及遗传育种研究中的应用[J].塔里木大学学报,2006,vol.18 No23:39-44
[199]文晓鹏.厐晓明,邓秀新.刺梨及部分近缘种形态学性状和RAPD分析[J].园艺学报,2003,30(2):204-206
[200]扬亚洲,王跃进,张剑侠等.中国葡萄属野生种抗旱基因的分子标记及遗传分析[J].园艺学报,2007,34(5):1087-1092
[201]吴锦程,杨向辉,林顺权等.枇杷AFLP分析体系的建立与应用[J].果树学报,2006,23(5):774-778.
[202]王斐,林盛华,方成泉等.梨新品种及其亲本的AFLP分析[J].园艺学报,2007,34(4):847-852
[203]江东,李喜庆,熊光明等.DNA Extraction Method for AFLP Analysis in Citrus[J]果树学报)2002,19(4)267-268.
[204]Moller EM,Bahnweg G,A simpleand efficient protocol for isolation of high molecular weight DNA from fiamentous fungi,fruit bodies,and infected plant tissues[J].Nucleic Acids Research,1992,20(22):6115-6116.
[205]GREEN,R.M.,VARDI,A.and E.GALUN.The plastome of Citrus physical map,Variation among Citrus cultivars and species and comparison with related genera thoer[m].Appl.genet.72:170-177
[206]SWINGLE,W.T.and P.C.REECE.The Botany of Citrus and Its Wild Relatives.[m]Citrus Industry Vol.Ⅰ 1967:279-381
[207]He.S.W,Liu,G R,and Xiang.D.M.On Three Problems in The Study of Germplasm Resources of Citrus[m].Proc Int Soc.Citriculture,Vol Ⅰ 1984:100-103,
[208]刘庚峰,贺善文,李文斌.莽山野橘.我国南岭山脉又一柑橘野生新种[J].湖南园艺,1987:1-4
[209]刘庚峰,贺善文,李文斌.中国真正柑橘亚属两野生新种[C].全国果树种质资源学术讨论会中国园艺学会,1987:43-45
[210]李文斌,贺善文,刘庚峰.湖南野生柑橘类种质过氧化物酶同功酶分析[J].园艺学报1987,14(3):151-160
[211]陈梦龙.湖南几种野生宽皮柑橘的亲缘关系研究.植物学性状调查与花粉粒形态观察[C].全国果树种质资源学术讨论会.中国园艺学会,1987:26-29
[212]易干军,谭卫萍,霍合强.龙眼品种种(系)遗传多样性及亲缘关系的AFLP分析[J].园艺学报,2003,30(3):272-276
[213]易干军,霍合强,陈大成,等.荔枝品种亲缘关系的AFLP分析[J].园艺学报,2003,30(4):399-403
[214]田亚南,柯穗,李韬等.应用电镜与PCR技术检测珀溪蜜袖黄龙病病原[J].植物病理学报,2000,30(1):76-81.
[215]李农一,唐庚元,毛晓林.柑橘木虱在湖南省的分布[J].植物检疫,1992(6)5:326-328.