摘要
目的:
观察研究内皮细胞特异性分子-1(endocellular specific molecule-1,ESM-1)在喉癌组织中的表达及与临床病理特征的关系,探讨其表达改变在肿瘤发生、侵袭、转移中可能机制,为喉癌临床诊断、治疗提供依据。
方法:
采用免疫组织化学的方法对50例喉鳞癌及40例癌旁组织中ESM-1的表达进行检测,分析ESM-1的表达与喉癌临床病理特征的关系。同时收集20例新鲜喉癌及15例新鲜癌旁组织标本,采用Westernblot方法检测ESM-1的蛋白表达水平,运用RT-PCR方法检测ESM-1mRNA的表达水平。
结果:
免疫组织化学方法检测表明,喉鳞癌组织中ESM-1表达显著高于癌旁对照组织(P<0.01);Ⅲ、Ⅳ期喉鳞癌组织中ESM-1的表达明显高于Ⅰ、Ⅱ期(P<0.05);伴有颈淋巴结转移的喉鳞癌组织中ESM-1表达明显高于无淋巴结转移的标本(P<0.05)。Western blot结果显示喉鳞癌组织中ESM-1蛋白表达明显高于癌旁组织,差别有统计学意义(P<0.01);RT-PCR检测表明喉鳞癌中ESM-1 mRNA的表达明显高于喉癌旁组织,差别有统计学意义(P<0.01)。
结论:
ESM-1在喉鳞癌组织中高表达,并且其表达程度与喉鳞癌的分期及是否存在淋巴结转移有关,提示ESM-1的表达与喉鳞癌的发生、侵袭、转移可能相关,可作为喉鳞癌预后判断的参考指标,并提供肿瘤干预治疗的靶点。
Objective:
To investigate the expression of human endocellular-specific molecule-1(ESM-1)in laryngeal carcinoma tissues and the relationship between ESM-1 expression and clinicopathological parameters,to study the action of ESM-1 in carcinogenesis,invasion and metastasis of tumor. Then look for a new target for diagnosis and treatment of laryngeal carcinoma
Methods:
The expression of ESM-1 in 50 specimens of laryngeal carcinoma and 40 specimens of para-carcinoma tissue was measured by the method of immunohistochemistry.The relationship between ESM-1 expression and clinicopathological parameters of laryngeal carcinoma was analyzed. Meanwhile,western blot was performed to detect the expression of ESM-1 in 20 fresh specimens of laryngeal carcinoma and 15 specimens of para-carcinoma tissue.Then RT-PCR was performed to detect ESM-1 mRNA expression.
Result:
The immunohistochemistry stain suggested that The qualitative expression of ESM-1 in laryngeal carcinoma tissues was significantly higher than that in para-carcinoma tissues(P<0.01);it also has a higher expression inⅢ、Ⅳstage thanⅠ、Ⅱstage of laryngeal carcinoma.The positive expression of ESM-1 was also significantly correlated with carcinomatous metastasis.Western blot and RT-PCR also suggested that there was a higher expression of both mRNA and protein of ESM-1 in laryngeal carcinoma tissues than in para-carcinoma tissues(P<0.01).
Conclusion:
In laryngeal carcinoma tissues,the expression of ESM-1 was higher than in para-carcinoma tissue and significantly related to metastasis and clinical stage,which may contribute to the carcinogenesis,development and invasion of laryngeal carcinoma.So ESM-1 may be an important index for judging prognosis of laryngeal carcinoma and provide a new target for cancer treatment.
引文
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