高番茄红素性状的遗传分析及分子标记
摘要
番茄红素是番茄重要的品质指标之一。番茄红素含量越高,品质越好。最近研究还表明番茄红素具有降低人类患某些恶性肿瘤风险的保健功能。因此,通过遗传改良,增加果实番茄红素的含量成为番茄品质育种的重要目标。
本试验利用高番茄红素含量的07009与低番茄红素含量的07922番茄品种为试验材料,以该组合的F2群体作为作图和遗传规律鉴定群体,对亲本和F2群体分离后代的番茄红素含量进行了鉴定,采用SSR分子标记构建遗传连锁图谱,利用复合区间定位方法对番茄红素含量性状进行QTL定位。主要研究成果如下:
1.对番茄中番茄红素含量进行遗传分析,结果表明:番茄红素含量在F2分离群体中,呈现连续性变异,χ2测验均不显著。据此判断番茄红素含量属于数量性状。ABC尺度遗传检验表明,番茄红素含量符合加性-显性遗传模型。番茄红素的狭义遗传力为44.47%,广义遗传力为75.8%。
2.应用SSR分子标记技术筛选得到与番茄红素含量相关的12个SSR标记。利用复合区间作图法在第9连锁群上检测到2个与番茄红素含量相关的QTLs。Qchl1与分子标记SSR237、T24紧密连锁,与二者分别相距7.0 cM和10.1 cM变异贡献率为7.23%。Qchl2与分子标记T24、FR119紧密连锁,与二者分别相距12.5 cM和6.1 cM,变异贡献率为10.64%。
3.通过对30份种质资源的分析鉴定,将30份番茄种质资源分成2组,高番茄红素含量组和低番茄红素含量组的分组准确率均为100%,由此可初步证明,对番茄红素含量性状QTL位点的SSR标记结果真实可靠。
Lycopene is one of the important characters for tomoto quality.Moreover,lycopene play the function on reducing the risk of certain types of cancer including carcinogenic,alimentary canal,respiratory tract and cataract formation.therefore,to increase lycopene content has been a major objective in tomato breeding.
A F2 population derived from 07009,a tomato inbred line with high lycopene content, and 07922, a cultivar with low lycopene content,was used to identity quantitative trait loci(QTL) associated with tomato lycopene content. lycopene content of parents and F2 population were identified.in addition,a molecular marker linkage mao was constructed with SSRmarker,and QTLananlysis was performed with composite interval mapping(CIM).The main results are as followe:
1. The genetic analysis of Lycopene Content in tomato has been carried out.Result indicates that:Theχ2 test of the Lycopene Content in tomato breeding showed the Lycopene Content was continual variation,theχ2 test was not significant,and come to the comclusion that the Lycopene Content in tomato was belong to the quantitative trait.The analysis of ABCshowed:the character of the Lycopene Content in tomato was fit for a model of additive-dominance, the broad heritability was 75.8% and the narrow heritability was 44.47%.
2. Twelve polymorphic SSR markers were used for QTLdetection .Qchl1 was linked with SSR markers SSR237 and T24 by 7.0 cM and 10.1 cM,respectively.It explained 7.23% of phenotypic variation. Qchl2 was linked with SSR markers T24 and FR119 by 12.5cM and 6.1 cM,respectively.It explained 10.64% of phenotypic variation.
3.The result of 30 germplasm identification is according with field performance,the high lycopene content and the low lycopene content,and the veracity of grouping were 100% , Based on this we can make a initiative conclusion that is the SSRmarket result which is about lycopene content character QTL position is correct..
本试验利用高番茄红素含量的07009与低番茄红素含量的07922番茄品种为试验材料,以该组合的F2群体作为作图和遗传规律鉴定群体,对亲本和F2群体分离后代的番茄红素含量进行了鉴定,采用SSR分子标记构建遗传连锁图谱,利用复合区间定位方法对番茄红素含量性状进行QTL定位。主要研究成果如下:
1.对番茄中番茄红素含量进行遗传分析,结果表明:番茄红素含量在F2分离群体中,呈现连续性变异,χ2测验均不显著。据此判断番茄红素含量属于数量性状。ABC尺度遗传检验表明,番茄红素含量符合加性-显性遗传模型。番茄红素的狭义遗传力为44.47%,广义遗传力为75.8%。
2.应用SSR分子标记技术筛选得到与番茄红素含量相关的12个SSR标记。利用复合区间作图法在第9连锁群上检测到2个与番茄红素含量相关的QTLs。Qchl1与分子标记SSR237、T24紧密连锁,与二者分别相距7.0 cM和10.1 cM变异贡献率为7.23%。Qchl2与分子标记T24、FR119紧密连锁,与二者分别相距12.5 cM和6.1 cM,变异贡献率为10.64%。
3.通过对30份种质资源的分析鉴定,将30份番茄种质资源分成2组,高番茄红素含量组和低番茄红素含量组的分组准确率均为100%,由此可初步证明,对番茄红素含量性状QTL位点的SSR标记结果真实可靠。
Lycopene is one of the important characters for tomoto quality.Moreover,lycopene play the function on reducing the risk of certain types of cancer including carcinogenic,alimentary canal,respiratory tract and cataract formation.therefore,to increase lycopene content has been a major objective in tomato breeding.
A F2 population derived from 07009,a tomato inbred line with high lycopene content, and 07922, a cultivar with low lycopene content,was used to identity quantitative trait loci(QTL) associated with tomato lycopene content. lycopene content of parents and F2 population were identified.in addition,a molecular marker linkage mao was constructed with SSRmarker,and QTLananlysis was performed with composite interval mapping(CIM).The main results are as followe:
1. The genetic analysis of Lycopene Content in tomato has been carried out.Result indicates that:Theχ2 test of the Lycopene Content in tomato breeding showed the Lycopene Content was continual variation,theχ2 test was not significant,and come to the comclusion that the Lycopene Content in tomato was belong to the quantitative trait.The analysis of ABCshowed:the character of the Lycopene Content in tomato was fit for a model of additive-dominance, the broad heritability was 75.8% and the narrow heritability was 44.47%.
2. Twelve polymorphic SSR markers were used for QTLdetection .Qchl1 was linked with SSR markers SSR237 and T24 by 7.0 cM and 10.1 cM,respectively.It explained 7.23% of phenotypic variation. Qchl2 was linked with SSR markers T24 and FR119 by 12.5cM and 6.1 cM,respectively.It explained 10.64% of phenotypic variation.
3.The result of 30 germplasm identification is according with field performance,the high lycopene content and the low lycopene content,and the veracity of grouping were 100% , Based on this we can make a initiative conclusion that is the SSRmarket result which is about lycopene content character QTL position is correct..
引文
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邓宇,张卫强. 2002.番茄红素提取方法的研究.现代化工. 22(2):25～28
范丙友,刘玉梅,高水平.2004.AFLP技术在蔬菜作物遗传育种研究上的应用.北方园艺2: 50～51
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郭绍贵,许勇,张海英等. 2005.不同环境效应对西瓜果实可溶性固形物含量的QTL影响.中国蔬菜. 13～16.
国艳梅. 2006.源于多毛番茄控制果实颜色等性状的QTL定位及相关基因的研究.北京:中国农业科学院.
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