水稻不同抗性品种抗稻瘟病生理生化机制的研究
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摘要
本研究以普粘7、龙选9707、空育131和丽江新团黑谷4个品种为材料,3个抗性品种分别接种相对应的弱毒力菌株,探讨了水稻幼苗期叶片中各种生化因子与抗瘟性的关系。采用比色法测定叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、抗坏血酸过氧化物酶(APX)和过氧化氢酶(CAT)的活性及丙二醛(MDA)含量,采用聚丙烯酰胺凝胶电泳分析POD、SOD、酯酶(EST)等同工酶变化,采用考马斯亮兰G-250法测定可溶性蛋白质含量,采用SDS-聚丙烯酰胺凝胶电泳分析蛋白质的变化,采用蒽酮法测定可溶性糖含量等。
本试验筛选得到空育131、龙选9707和普粘7对应弱毒菌株分别为LBDM-2、LBTJ-3和M1032。测得三个抗性品种表现一定的抗扩展能力。
研究结果表明,MDA的含量和POD、PPO、PAL、APX、CAT等活性是水稻抗瘟性重要机制之一,在水稻幼苗期,MDA含量稳定有利于水稻抗稻瘟病;CAT活性在发生抗瘟性反应时表现为下降,其它酶活性在发生抗瘟性反应时表现为升高。水稻幼苗期可溶性糖类和蛋白质含量在发生抗瘟性反应时表现为明显升高。不同处理EST同工酶和SOD同工酶电泳谱带几乎没有差异,二者与抗瘟性无关。不同品种在各生化因子含量和表现上有一定的差异。
In this study,we used three rice varieties Punian7,Longxuan9707 and Kongyu 131 that respectively,one susceptible variety Li jiangxingu that did not conclude anti-disease genes.We studied the relationship between blast resistance and various physiological and biochemical factors in rice seeding stage,such as determining anti-expansion of all varieties by DD Act,the content of MDA in leaves and activities of POD、PPO、PAL、APX and CAT by colorimetry, the content of soluble ptotein by coomassie brilliant-G-250, of the content of soluble sugar by anthrone method,analysing changes of POD、SOD and EST by polyacrylamide gelectrophoresis.
In this experiment we got the corresponding low-toxicity strains of Kongyu 131、Longxuan9707 and Punian7 were LBDM-2、LBTJ-3 and M1032 by streening. The three resistant varieties expressed anti-expansion capacity.
The results showed that,the content of MDA, activities of SOD、POD、PPO、PAL、APX and CAT were all important mechanisms of rice resistance to blast .In rice seedling stage,the stable content of MDA is helpful to rice blast resistance, the CAT activity declined in performance when the reaction of rice resistance to blast occured,and other E activities increased in performance. In rice seedling stage the content of soluble sugar and soluble protein increased obviously when the reaction of rice resistance to blast occurred .There were no bands occurring in the electrophoresis of isozenzyme EST and SOD,they had nothing to do with the blast resistance.Different varieties performed differently in various biochemical factors.
本试验筛选得到空育131、龙选9707和普粘7对应弱毒菌株分别为LBDM-2、LBTJ-3和M1032。测得三个抗性品种表现一定的抗扩展能力。
研究结果表明,MDA的含量和POD、PPO、PAL、APX、CAT等活性是水稻抗瘟性重要机制之一,在水稻幼苗期,MDA含量稳定有利于水稻抗稻瘟病;CAT活性在发生抗瘟性反应时表现为下降,其它酶活性在发生抗瘟性反应时表现为升高。水稻幼苗期可溶性糖类和蛋白质含量在发生抗瘟性反应时表现为明显升高。不同处理EST同工酶和SOD同工酶电泳谱带几乎没有差异,二者与抗瘟性无关。不同品种在各生化因子含量和表现上有一定的差异。
In this study,we used three rice varieties Punian7,Longxuan9707 and Kongyu 131 that respectively,one susceptible variety Li jiangxingu that did not conclude anti-disease genes.We studied the relationship between blast resistance and various physiological and biochemical factors in rice seeding stage,such as determining anti-expansion of all varieties by DD Act,the content of MDA in leaves and activities of POD、PPO、PAL、APX and CAT by colorimetry, the content of soluble ptotein by coomassie brilliant-G-250, of the content of soluble sugar by anthrone method,analysing changes of POD、SOD and EST by polyacrylamide gelectrophoresis.
In this experiment we got the corresponding low-toxicity strains of Kongyu 131、Longxuan9707 and Punian7 were LBDM-2、LBTJ-3 and M1032 by streening. The three resistant varieties expressed anti-expansion capacity.
The results showed that,the content of MDA, activities of SOD、POD、PPO、PAL、APX and CAT were all important mechanisms of rice resistance to blast .In rice seedling stage,the stable content of MDA is helpful to rice blast resistance, the CAT activity declined in performance when the reaction of rice resistance to blast occured,and other E activities increased in performance. In rice seedling stage the content of soluble sugar and soluble protein increased obviously when the reaction of rice resistance to blast occurred .There were no bands occurring in the electrophoresis of isozenzyme EST and SOD,they had nothing to do with the blast resistance.Different varieties performed differently in various biochemical factors.
引文
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