金花茶降低衰老机体动脉粥样硬化风险的作用
摘要
目的探讨金花茶降低衰老机体动脉粥样硬化发病风险的作用。方法雄性SD大鼠40只,体重180-220克,随机分成4组,每组10只,分别为正常对照组(N组),衰老模型组(A组),高浓度金花茶组(HC组)和低浓度金花茶组(LC组)。模型组和2组金花茶组采用D-半乳糖连续腹腔注射200mg/(kg·d)制备亚急性衰老动物模型,1次/d,共40天。衰老模型组造模成功后不再做任何处理。金花茶用药组大鼠造模成功后,低浓度组每天用金茶花2g/kg煮水灌胃,高浓度组每天用4g/kg煮水灌胃,1次/d,共40天。正常对照组大鼠每天灌胃同等剂量的生理盐水,时间同金花茶组大鼠。最后一次灌胃后取大鼠尾部静脉血测TC、TG、HDL-C、LDL-C含量,然后断颈处死大鼠,取肝脏组织测MDA、SOD、TC、TG含量及bax、be1-2mRNA表达水平。结果(1)衰老模型组肝脏MDA含量较正常对照组明显升高(P<0.05)而SOD含量较正常对照组明显降低(P<0.05)；高、低浓度金花茶组肝脏MDA含量较衰老模型组明显降低(P<0.05)而SOD含量较正常对照组明显升高(P<0.05)；高、低浓度金花茶组间比较,上述指标有统计学差异(P<0.05)。(2)衰老模型组血清HDL-C含量较正常对照组明显降低(P<0.05)而血清TC、TG、LDL-C含量较正常对照组明显升高(P<0.05)；高、低浓度金花茶组血清HDL-C含量较衰老模型组显著升高(P<0.05)且血清TC、TG、LDL-C含量较衰老模型组明显下降(P<0.05),高、低浓度金花茶组间比较,上述指标有统计学差异(P<0.05)。(3)衰老模型组肝脏TC、TG含量较正常对照组明显升高(P<0.05)；高、低浓度金花茶组肝组织TC、TG含量较衰老模型组明显下降(P<0.05),高、低浓度金花茶组间比较,上述指标有统计学差异(P<0.05)。(4)衰老模型组肝脏be1-2mRNA表达水平较正常对照组明显降低(P<0.05)而bax mRNA表达水平较对照组明显降低(P<0.05)；高、低浓度金花茶组肝脏be1-2mRNA表达水平较衰老模型组显著升高(P<0.05)且bax mRNA表达水平较模型组明显下降(P<0.05),高、低浓度金花茶组间比较,上述指标有统计学差异(P<0.05)。结论金花茶可能通过提高SOD含量和减少MDA堆积,上调bcl-2和下调bax mRNA表达水平,减慢肝脏凋亡,以延缓机体衰老；并调节血脂,降低TC、TG、LDL-C,增加HDL-C含量,延缓肝细胞凋亡,提高肝细胞活性并减少肝脏脂肪堆积,以降低衰老进程中动脉粥样硬化的发病风险。
Objective:Discussion on the role of Camellia chrysantha prevent atherosclerosis in the aging body. Methods:40male SD rats, weight180-220g, randomly divided into4groups, each group of10, normal control group (N), respectively, aging group (A), high concentrations of Camellia chrysantha group(HC) and low concentrations of Camellia chrysantha group(LC). Group of drugs used in model groups and2group d-Galactose successive intraperitoneal injection of200mg/(kg·d) preparation of animal model of acute senile,1/d, a total of40days. A group modeled after the success of the model is no longer doing anything. Tea set of rat model of drug use after the success of Camellia chrysantha one set every day intragastric infusion with2g/kg boiled water, the other one group4g/kg intragastric infusion with water every day,1/d, a total of40days. Intragastric infusion with N group rats every day the same dose of normal saline, groups of rats with medication. Tail vein in rats after intragastric infusion with last access contents of blood testing TC, TG, HDL-C, LDL-C, and broken neck executed preparation of liver tissue of rats measured MDA, SOD, TC, TG content and expression of bax, bcl-2mRNA levels.Results:(1)The A group liver MDA levels significantly elevated compared with N group (P<0.05) and SOD contents significantly lower than N group(P<0.05); HC and LC group significantly reduced liver MDA levels than the A group(P<0.05) and SOD contents significantly higher than N group(P<0.05); Comparison between HC and LC group, the indicators there are statistically significant differences (P<0.05).(2) the A group serum HDL-C levels significantly lower than N groups (P<0.05) and serum TC and TG, LDL-C content significantly higher than N group(P<0.05);HC and LC group HDL-C content model for A group significantly increased (P<0.05) and serum TC and TG, LDL-C content than the A group decreased markedly (P<0.05), between the HC and LC group, the indicators there are statistically significant differences (P<0.05).(3) the liver of A group TC and TG levels significantly elevated compared with N group (P<0.05); HC and LC groups TC, TG content than the A group decreased markedly (P<0.05), between the HC and LC group, the indicators there are statistically significant differences (P<0.05).(4) the A group of bcl-2mRNA expression in liver levels significantly lower than N groups (P<0.05) and the expression of bax mRNA levels significantly lower than N groups (P<0.05); Comparison between HC and LC group, the indicators there are statistically significant differences (P<0.05). Conclusions:Camellia chrysantha by increasing the content of SOD and MDA accumulation reduction, increase bcl-2and reduced expression of bax mRNA levels, slow down liver apoptosis, delaying the senescence of organism; And may by regulation of blood lipid, lower TC, TG, LDL-C, increased HDL-C content, slow down liver cell apoptosis, increase the activity of liver cells and reduce the fat accumulation in liver, to reduce the risk of atherosclerosis in the aging process.
Objective:Discussion on the role of Camellia chrysantha prevent atherosclerosis in the aging body. Methods:40male SD rats, weight180-220g, randomly divided into4groups, each group of10, normal control group (N), respectively, aging group (A), high concentrations of Camellia chrysantha group(HC) and low concentrations of Camellia chrysantha group(LC). Group of drugs used in model groups and2group d-Galactose successive intraperitoneal injection of200mg/(kg·d) preparation of animal model of acute senile,1/d, a total of40days. A group modeled after the success of the model is no longer doing anything. Tea set of rat model of drug use after the success of Camellia chrysantha one set every day intragastric infusion with2g/kg boiled water, the other one group4g/kg intragastric infusion with water every day,1/d, a total of40days. Intragastric infusion with N group rats every day the same dose of normal saline, groups of rats with medication. Tail vein in rats after intragastric infusion with last access contents of blood testing TC, TG, HDL-C, LDL-C, and broken neck executed preparation of liver tissue of rats measured MDA, SOD, TC, TG content and expression of bax, bcl-2mRNA levels.Results:(1)The A group liver MDA levels significantly elevated compared with N group (P<0.05) and SOD contents significantly lower than N group(P<0.05); HC and LC group significantly reduced liver MDA levels than the A group(P<0.05) and SOD contents significantly higher than N group(P<0.05); Comparison between HC and LC group, the indicators there are statistically significant differences (P<0.05).(2) the A group serum HDL-C levels significantly lower than N groups (P<0.05) and serum TC and TG, LDL-C content significantly higher than N group(P<0.05);HC and LC group HDL-C content model for A group significantly increased (P<0.05) and serum TC and TG, LDL-C content than the A group decreased markedly (P<0.05), between the HC and LC group, the indicators there are statistically significant differences (P<0.05).(3) the liver of A group TC and TG levels significantly elevated compared with N group (P<0.05); HC and LC groups TC, TG content than the A group decreased markedly (P<0.05), between the HC and LC group, the indicators there are statistically significant differences (P<0.05).(4) the A group of bcl-2mRNA expression in liver levels significantly lower than N groups (P<0.05) and the expression of bax mRNA levels significantly lower than N groups (P<0.05); Comparison between HC and LC group, the indicators there are statistically significant differences (P<0.05). Conclusions:Camellia chrysantha by increasing the content of SOD and MDA accumulation reduction, increase bcl-2and reduced expression of bax mRNA levels, slow down liver apoptosis, delaying the senescence of organism; And may by regulation of blood lipid, lower TC, TG, LDL-C, increased HDL-C content, slow down liver cell apoptosis, increase the activity of liver cells and reduce the fat accumulation in liver, to reduce the risk of atherosclerosis in the aging process.
引文
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