射干的化学成分研究及其药动学初步探索
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摘要
射干是鸢尾科射干属植物射干Belamcanda Chinensis(L.)DC的干燥根茎,为常用中药,具有抗炎、止咳、祛痰等作用。近年来很多国家对其化学成分、药理作用进行了大量深入的研究,进一步阐明了射干临床疗效的内在基础,并在此基础上研发新药和保健用品,已取得相关专利和成果。这充分说明了射干具有确切的作用,前景令人乐观,值得进行深入研究。本文从化学成分及其药动学上对其进行研究与探索。
射干中含有丰富的异黄酮,且具有多种药理活性,本文利用硅胶柱色谱,聚酰胺柱色谱,凝胶柱色谱,高效液相色谱,重结晶等多种分离手段对射干的乙醇提取物进行分离,从中分离得到14个化合物。通过波谱解析及理化方法鉴定了其中10个化合物的化学结构。其中异黄酮类化合物5个,甾体类化合物2个,生物碱类化合物1个,三萜类化合物1个,小分子类化合物1个。分别为:次野鸢尾黄素(irisflorentin 1),白射干素(dichotomitin 2),野鸢尾黄素(irigenin 3),鸢尾苷元(tectorigenin 4),3′,5′-二甲氧基鸢尾素-4′-O-β-D-葡萄糖苷(3′,5′-dimethoxy irisolone-4′-O-β-D-glucoside 5),β-胡萝卜苷(β-daucosterol 6),β-谷甾醇(β-sitosterol 7),尿嘧啶核苷(uridine 8),环阿尔廷醇(cycloartanol9),罗布麻宁(apocynin 10),其中化合物9为首次从该属植物中分离得到的已知化合物。
建立了测定大鼠血浆中次野鸢尾黄素含量的RP-HPLC方法,以芹菜素为内标,采用C_(18)色谱柱,甲醇-水(60:40 v/v)为流动相,流速1.0 ml/min,检测波长265 nm。结果未检测到样品,对射干中异黄酮类化合物的药动学特征有了初步的了解,为进一步研制中药复方制剂的药动学行为奠定了基础。
The rhizome of Belamcanda chinensis (L.) DC that belongs to Iridacea, It has been used in Chinese herbs for the treatment of antitussive antiinflammatory and expectorant. It has been studied in many countries, on its pharmacological effects. From the results, the innate bases of its clinical effects are clarified further more, according to which, people developed many new medicines and health products. Those strongly speak volumes for its exact effects, and good prospects, so it is worthy of doing further studies on it. The author has investigated and explored the chemical constitutes and pharmacokinetics.
The rhizome of Belamcanda chinensis (L.) DC is rich in isoflavonoids, and they have many pharmacological effects. 14 compounds from the ethanol extraction were isolated by silica gel, polyamide, Sephadex LH-20, HPLC, recrystallizing. On the basis of NMR, and measuring physic-chemical characters, 10 chemical structures were elucidated as irisflorentin (1),dichotomitin(2),irigenin(3),tectorigenin (4), 3',5'-dimethoxy irisolone-4'-O-β-D-glucoside (5),β-daucosterol(6),β-sitosterol(7), uridine(8), cycloartanol(9), apocynin(10), respectively. Of all these compounds, compound 9 was isolated for the first time from Belamcanda,
RP-HPLC method has been developed to determine irisflorentin in rat plasma. Irisflorentin and apigenin (an internal standard) were extracted from plasma using liquid-liquid extraction with acetoacetate, they were separated on a C_(18) column. Using a mixture of methanol-water (60:40, v/v) as the mobile phase at a flow rate of 1.0 ml/min with UV detection at 265 nm. The Results show that the sample has not been detected. Preliminary understanding the features of pharmacokinetics of isoflavonoids from Belamcanda chinensis (L.) DC. It laid the foundation for future development of the Pharmacokinetics acts of Chinese medicine compound preparation.
射干中含有丰富的异黄酮,且具有多种药理活性,本文利用硅胶柱色谱,聚酰胺柱色谱,凝胶柱色谱,高效液相色谱,重结晶等多种分离手段对射干的乙醇提取物进行分离,从中分离得到14个化合物。通过波谱解析及理化方法鉴定了其中10个化合物的化学结构。其中异黄酮类化合物5个,甾体类化合物2个,生物碱类化合物1个,三萜类化合物1个,小分子类化合物1个。分别为:次野鸢尾黄素(irisflorentin 1),白射干素(dichotomitin 2),野鸢尾黄素(irigenin 3),鸢尾苷元(tectorigenin 4),3′,5′-二甲氧基鸢尾素-4′-O-β-D-葡萄糖苷(3′,5′-dimethoxy irisolone-4′-O-β-D-glucoside 5),β-胡萝卜苷(β-daucosterol 6),β-谷甾醇(β-sitosterol 7),尿嘧啶核苷(uridine 8),环阿尔廷醇(cycloartanol9),罗布麻宁(apocynin 10),其中化合物9为首次从该属植物中分离得到的已知化合物。
建立了测定大鼠血浆中次野鸢尾黄素含量的RP-HPLC方法,以芹菜素为内标,采用C_(18)色谱柱,甲醇-水(60:40 v/v)为流动相,流速1.0 ml/min,检测波长265 nm。结果未检测到样品,对射干中异黄酮类化合物的药动学特征有了初步的了解,为进一步研制中药复方制剂的药动学行为奠定了基础。
The rhizome of Belamcanda chinensis (L.) DC that belongs to Iridacea, It has been used in Chinese herbs for the treatment of antitussive antiinflammatory and expectorant. It has been studied in many countries, on its pharmacological effects. From the results, the innate bases of its clinical effects are clarified further more, according to which, people developed many new medicines and health products. Those strongly speak volumes for its exact effects, and good prospects, so it is worthy of doing further studies on it. The author has investigated and explored the chemical constitutes and pharmacokinetics.
The rhizome of Belamcanda chinensis (L.) DC is rich in isoflavonoids, and they have many pharmacological effects. 14 compounds from the ethanol extraction were isolated by silica gel, polyamide, Sephadex LH-20, HPLC, recrystallizing. On the basis of NMR, and measuring physic-chemical characters, 10 chemical structures were elucidated as irisflorentin (1),dichotomitin(2),irigenin(3),tectorigenin (4), 3',5'-dimethoxy irisolone-4'-O-β-D-glucoside (5),β-daucosterol(6),β-sitosterol(7), uridine(8), cycloartanol(9), apocynin(10), respectively. Of all these compounds, compound 9 was isolated for the first time from Belamcanda,
RP-HPLC method has been developed to determine irisflorentin in rat plasma. Irisflorentin and apigenin (an internal standard) were extracted from plasma using liquid-liquid extraction with acetoacetate, they were separated on a C_(18) column. Using a mixture of methanol-water (60:40, v/v) as the mobile phase at a flow rate of 1.0 ml/min with UV detection at 265 nm. The Results show that the sample has not been detected. Preliminary understanding the features of pharmacokinetics of isoflavonoids from Belamcanda chinensis (L.) DC. It laid the foundation for future development of the Pharmacokinetics acts of Chinese medicine compound preparation.
引文
[1]吉文亮,秦民坚.中药射干的化学与药理研究进展[J].国外医药:植物药分册,2000,15(2):57-58.
[2]邱鹰昆,高玉白,徐碧霞等.射干异黄酮类化合物的分离与结构鉴定[J].中国药物化学杂志,2006,16(3):175-177.
[3]吉文亮,秦民坚,王诤涛.射干的化学成分研究(Ⅰ)[J].中国药科大学学报,2001,32(3):197-199.
[4]Hideyuki ITO,Satomi ONOUE,Takashi YOSHIDA.Isoflavonoids from Belameanda chinensis[J].Chem.Pharm.Bull,2001,49(9):1229-1231.
[5]吉文亮,秦民坚,王竫涛.射干的化学成分研究(Ⅱ)[J].中草药,2004,35(5):487-489.
[6]Orawan M.,Wanchai D.,Kaoru U.,et al.Phenolic Constituents of the Rhizomes of the Thai Medicinal Plant Belamcanda chinensis with Proliferative Activity for Two Breast Cancer Cell Lines[J].N.J Nat Prod,2005,68(3):361-364.
[7]邱鹰昆,高玉白,徐碧霞等.射干的化学成分研究[J].中国药学杂志,2006,41(15):1133-1135.
[8]李晓兰.中药射干的研究概况.海峡药学[J],2003,15(5):72-74.
[9]Fukiyama Y.Chem Pharm Bull,1991,39(7):1877.
[10]Yoshiyasu Fukuyama,Yuuko Kiriyama,Junko Okino,et al.Belamcandaquinones A and B,novel dimericl,4-benzoquinone derivatives possessing cyclooxygenase inhibitory activity[J].Tetrahedron Letters,1993,34(47):7633-7636.
[11]Hideyuki I,el al.J.Nat,Prod.,1999:62(1):89.
[12]Kunihiko Takahashfi,Yoji Hoshino,Sumiko Suzuki,et al.Iridals from Iris tectorum and Belamcanda chinensis[J]Photochemistry,2000,53(8):925-929.
[13]Kunihiko T,el al.Biol.Pharm.Bull.25(4)432-436.
[14]秦民坚,王强,徐珞珊等.射干和鸢尾的挥发性成分,植物资源与环境,1997,6(2):54-55.
[15]吴泽芳.射干与白射干、川射干的药理作用比较.药物分析杂志[J],1985,5(3):167-168.
[16].钟鸣,关旭俊,黄炳生等.中药射干现代研究进展[J].中药材,2001,24(12):904-907.
[17]Kim Y P,Yamada M,Lim S,et al.Inhibition by tectorigenin and tectoridin of prostaglandin E2production and cyclooxygenase-2 induction in rat peritoneal macrophages[J].Biochimica et biophysica acta.1999,1438(3):399-407.
[18]Ohuchi K,Kim Y P,Lim,et al.Inhibition of cyclooxygenase-2 induction in rat peritoneal macrophages by tectorigenin isolated from the rhizomes of Belamcanda chinensis,and its mechanism of action.Rece- nt Advances in Natural Products Research,Proceedings of the International Symposium on Recent Advances in Natural Products Research,3rd,Seoul,Republic of Korea,1999,19(1999):12-24.
[19]国家医药管理局中草药情报中心站编.植物药效成分分册.北京:人民卫生出版社,1986:612,696,1028.
[20]王吴.10种中药对治病性浅部真菌的抑制实验研究[J].中医药杂志,1997,38(7):431-432.
[21]刘春平,王凤荣,南国荣等.中药射干提取物对皮肤癣茵的抑菌作用研究[J].中华皮肤科杂志,1998,31(5):310-311.
[22]刘春平,南国荣,王凤荣等.射干乙醚部分抗红色毛癣菌的电镜观察[J].中华皮肤科杂志,1999,32(5):341-342.
[23]于军,徐丽华,王云等.射干和马齿苋对46株绿脓杆菌体外试验的研究[J].白求恩医科大学学报,2001,27(2):130-131.
[24]王云,于军,于红.射干提取液对绿脓杆菌体P_(29)朱R质粒体内外消除作用研究[J].长春中医学院学报,1999,15(3):64。
[25]Morrissey C,Bektic J,Spengler B,et al.Phytoestrogens derived from Belamcanda chinensis have an antiproliferative effect on prostate cancer cells in vitro[J].J Urol.2004 Dec;172(6 Pt 1):2426-33.
[26]Thelen P,Scharf JG,Burfeind P,etal.Tectorigenin and other phytochemicals extracted from leopard lily Belamcanda chinensis affect new and established targets for therapies in prostate cancer[J].Carcinogenesis.(2):2005 Aug;26(8):1360-7.
[27]王红武,张明发,沈雅琴等.射干对消化系统及实验性血栓的影响[J].中医研究,1997,13(5):43-45.
[28]李宁.射干致泻与用量浅谈[J].中国中医药杂志,1991,16(4):249.
[29]Hideyuki I,et al.J Nat Prod,1999,62(1):89.
[30]纪志武等。乌柏、射干和巴豆油对3-甲基胆蒽诱发的小白鼠皮肤肿瘤的促进作用研究[J].癌症,1989,8(5):350-351.
[31]Fumiko Abe,Rong-Fu Chen,Tatsuo Yamauchi.Iridals from Belarncanda chinensis and Iris japonica [J].Photochemistry,1991,30(10):3379-3382.
[32]Jung SH,Lee YS,Lim SS,et al.Arch Pharm Res.Antioxidant activities of isoflavones from the rhizomes of Belamcanda chinensis on carbon tetrachloride-induced hepatic injury in rats[J].2004,27(2):184-8.
[33]蔡柳洲.射干瓜蒌饮治疗慢性胃炎68例[J].北京中医.1991(5):20-21.
[34]邱志济,邱江峰,邱江东等.自拟速效肝丹治疗高酶高疸急性乙肝举隅[J].实用中医杂志,1997,13(4):24-25.
[35]姜树兰.中药治疗外阴白色病损病380例[J].陕西中医,1986,(5):199-201.
[36]郝玉凤,田美蓉等.中西医结合治疗慢性外阴营养不良86例临床报告[J].陕西中医学院学报,1999,22(1):27-28.
[37]李学军,浦琴,郑茂斌等.百部等五味中药复方乙醇提取物治疗伤科创面感染临床疗效观察[J].贵阳中医学院学报,1998,20(3):47-48.
[38]吉照春.特制脚气灵癣药水治疗足癣580例[J].陕西中医,1994,15(11):505.
[39]王毫.射干散治疗阳痿50例[J].实用中医内科杂志,1999,13(4):32.
[40]Kawaii S,Tomono Y,Ogawa K,Sugiura M,et al.Antiproliferative effect of isopenteny lated coumarins on several cancer cell lines.Anticancer Res.2001,21(33):1905-1911.
[41]郭立玮主编.中药药物动力学方法与应用.北京:人民卫生出版社,2002.
[42]张明,侯世祥,廖工铁等,复方中药制剂药物代谢动力学研究进展(Ⅱ)-研究成果,意义,方法评价与展望.华西药学杂志,1993,8(1):38-41.
[43]曹春林,中药剂型与生物药剂学的相关论述.中成药研究,1981,18(3):41-44.
[44]胡长鸿,施顺清,沈坚.关于中药剂型现代化问题.中成药研究,1986,(1):35-38.
[45]郭立玮,金妙文,加强中药新药生物制剂学研究的意义及措施.中药新药与临床药理,1996,7(1):43-45.
[46]韩立炜,任天池.中药有效成分药物动力学国内研究最新进展.中国中医药信息杂志,2000,7(9):16-19.
[47]赫梅生,詹丽芬,郭景阳等用动物急性死亡率法估计药物体存率与表观半衰期[J].中国药理学报,1985,6(3):213.
[48]雷帆,邢东明等.石榴叶提取物中揉花酸在大鼠体内药物动力学研究.药品评价,2005,2(1):38-41.
[49]邵瑞莹等.高效液相-质谱法联用测定大鼠体内去甲基斑鳌素血药浓度.药物分析杂志,2004,22(1):607-609.
[50]廖文强,沈月华等.毛细管区带电泳方法对大鼠体内地西浮血药浓度的测定.中国医院药学杂志,2002,22(1):9-10.
[51]张国辉,马辰.黄酮类化合物药动学研究进展.中草药,2004,35(5):582-585.
[52]李应勤,陆蕴茹,魏璐雪.白射干黄酮类成分的研究[J].药学学报,1986,21(11):836-841.
[53]Li Jin,Hai Sheng Chen,Zhao Bao Xiang,et al.New flavone and isoflavone glycoside from Belamcanda chinensis[J].Chinese Chemical Letters,2007,18(2):158-160.
[54]PENG Xian,TAN Li.Studies on the constimnets from the the Rhazoma of Alisma orientalis[J]. Chinese Pharmaceutical Sciencs,1998(3):173-174.
[55]乔春峰,李秋文,董辉等.闭鞘姜属两种植物的化学成分研究[J].中国中药杂志,2002,27(2):123-125.
[56]冯卫生,郑晓珂,刘云宝等.石胆草中黄酮碳苷的分离与结构鉴定[J].药学学报,2004,39(2):110-115.
[57]赵秀娟,吴坤.芹菜素代谢动力学研究进展[J].中国公共卫生,2007,23(8):1020-1021.
[2]邱鹰昆,高玉白,徐碧霞等.射干异黄酮类化合物的分离与结构鉴定[J].中国药物化学杂志,2006,16(3):175-177.
[3]吉文亮,秦民坚,王诤涛.射干的化学成分研究(Ⅰ)[J].中国药科大学学报,2001,32(3):197-199.
[4]Hideyuki ITO,Satomi ONOUE,Takashi YOSHIDA.Isoflavonoids from Belameanda chinensis[J].Chem.Pharm.Bull,2001,49(9):1229-1231.
[5]吉文亮,秦民坚,王竫涛.射干的化学成分研究(Ⅱ)[J].中草药,2004,35(5):487-489.
[6]Orawan M.,Wanchai D.,Kaoru U.,et al.Phenolic Constituents of the Rhizomes of the Thai Medicinal Plant Belamcanda chinensis with Proliferative Activity for Two Breast Cancer Cell Lines[J].N.J Nat Prod,2005,68(3):361-364.
[7]邱鹰昆,高玉白,徐碧霞等.射干的化学成分研究[J].中国药学杂志,2006,41(15):1133-1135.
[8]李晓兰.中药射干的研究概况.海峡药学[J],2003,15(5):72-74.
[9]Fukiyama Y.Chem Pharm Bull,1991,39(7):1877.
[10]Yoshiyasu Fukuyama,Yuuko Kiriyama,Junko Okino,et al.Belamcandaquinones A and B,novel dimericl,4-benzoquinone derivatives possessing cyclooxygenase inhibitory activity[J].Tetrahedron Letters,1993,34(47):7633-7636.
[11]Hideyuki I,el al.J.Nat,Prod.,1999:62(1):89.
[12]Kunihiko Takahashfi,Yoji Hoshino,Sumiko Suzuki,et al.Iridals from Iris tectorum and Belamcanda chinensis[J]Photochemistry,2000,53(8):925-929.
[13]Kunihiko T,el al.Biol.Pharm.Bull.25(4)432-436.
[14]秦民坚,王强,徐珞珊等.射干和鸢尾的挥发性成分,植物资源与环境,1997,6(2):54-55.
[15]吴泽芳.射干与白射干、川射干的药理作用比较.药物分析杂志[J],1985,5(3):167-168.
[16].钟鸣,关旭俊,黄炳生等.中药射干现代研究进展[J].中药材,2001,24(12):904-907.
[17]Kim Y P,Yamada M,Lim S,et al.Inhibition by tectorigenin and tectoridin of prostaglandin E2production and cyclooxygenase-2 induction in rat peritoneal macrophages[J].Biochimica et biophysica acta.1999,1438(3):399-407.
[18]Ohuchi K,Kim Y P,Lim,et al.Inhibition of cyclooxygenase-2 induction in rat peritoneal macrophages by tectorigenin isolated from the rhizomes of Belamcanda chinensis,and its mechanism of action.Rece- nt Advances in Natural Products Research,Proceedings of the International Symposium on Recent Advances in Natural Products Research,3rd,Seoul,Republic of Korea,1999,19(1999):12-24.
[19]国家医药管理局中草药情报中心站编.植物药效成分分册.北京:人民卫生出版社,1986:612,696,1028.
[20]王吴.10种中药对治病性浅部真菌的抑制实验研究[J].中医药杂志,1997,38(7):431-432.
[21]刘春平,王凤荣,南国荣等.中药射干提取物对皮肤癣茵的抑菌作用研究[J].中华皮肤科杂志,1998,31(5):310-311.
[22]刘春平,南国荣,王凤荣等.射干乙醚部分抗红色毛癣菌的电镜观察[J].中华皮肤科杂志,1999,32(5):341-342.
[23]于军,徐丽华,王云等.射干和马齿苋对46株绿脓杆菌体外试验的研究[J].白求恩医科大学学报,2001,27(2):130-131.
[24]王云,于军,于红.射干提取液对绿脓杆菌体P_(29)朱R质粒体内外消除作用研究[J].长春中医学院学报,1999,15(3):64。
[25]Morrissey C,Bektic J,Spengler B,et al.Phytoestrogens derived from Belamcanda chinensis have an antiproliferative effect on prostate cancer cells in vitro[J].J Urol.2004 Dec;172(6 Pt 1):2426-33.
[26]Thelen P,Scharf JG,Burfeind P,etal.Tectorigenin and other phytochemicals extracted from leopard lily Belamcanda chinensis affect new and established targets for therapies in prostate cancer[J].Carcinogenesis.(2):2005 Aug;26(8):1360-7.
[27]王红武,张明发,沈雅琴等.射干对消化系统及实验性血栓的影响[J].中医研究,1997,13(5):43-45.
[28]李宁.射干致泻与用量浅谈[J].中国中医药杂志,1991,16(4):249.
[29]Hideyuki I,et al.J Nat Prod,1999,62(1):89.
[30]纪志武等。乌柏、射干和巴豆油对3-甲基胆蒽诱发的小白鼠皮肤肿瘤的促进作用研究[J].癌症,1989,8(5):350-351.
[31]Fumiko Abe,Rong-Fu Chen,Tatsuo Yamauchi.Iridals from Belarncanda chinensis and Iris japonica [J].Photochemistry,1991,30(10):3379-3382.
[32]Jung SH,Lee YS,Lim SS,et al.Arch Pharm Res.Antioxidant activities of isoflavones from the rhizomes of Belamcanda chinensis on carbon tetrachloride-induced hepatic injury in rats[J].2004,27(2):184-8.
[33]蔡柳洲.射干瓜蒌饮治疗慢性胃炎68例[J].北京中医.1991(5):20-21.
[34]邱志济,邱江峰,邱江东等.自拟速效肝丹治疗高酶高疸急性乙肝举隅[J].实用中医杂志,1997,13(4):24-25.
[35]姜树兰.中药治疗外阴白色病损病380例[J].陕西中医,1986,(5):199-201.
[36]郝玉凤,田美蓉等.中西医结合治疗慢性外阴营养不良86例临床报告[J].陕西中医学院学报,1999,22(1):27-28.
[37]李学军,浦琴,郑茂斌等.百部等五味中药复方乙醇提取物治疗伤科创面感染临床疗效观察[J].贵阳中医学院学报,1998,20(3):47-48.
[38]吉照春.特制脚气灵癣药水治疗足癣580例[J].陕西中医,1994,15(11):505.
[39]王毫.射干散治疗阳痿50例[J].实用中医内科杂志,1999,13(4):32.
[40]Kawaii S,Tomono Y,Ogawa K,Sugiura M,et al.Antiproliferative effect of isopenteny lated coumarins on several cancer cell lines.Anticancer Res.2001,21(33):1905-1911.
[41]郭立玮主编.中药药物动力学方法与应用.北京:人民卫生出版社,2002.
[42]张明,侯世祥,廖工铁等,复方中药制剂药物代谢动力学研究进展(Ⅱ)-研究成果,意义,方法评价与展望.华西药学杂志,1993,8(1):38-41.
[43]曹春林,中药剂型与生物药剂学的相关论述.中成药研究,1981,18(3):41-44.
[44]胡长鸿,施顺清,沈坚.关于中药剂型现代化问题.中成药研究,1986,(1):35-38.
[45]郭立玮,金妙文,加强中药新药生物制剂学研究的意义及措施.中药新药与临床药理,1996,7(1):43-45.
[46]韩立炜,任天池.中药有效成分药物动力学国内研究最新进展.中国中医药信息杂志,2000,7(9):16-19.
[47]赫梅生,詹丽芬,郭景阳等用动物急性死亡率法估计药物体存率与表观半衰期[J].中国药理学报,1985,6(3):213.
[48]雷帆,邢东明等.石榴叶提取物中揉花酸在大鼠体内药物动力学研究.药品评价,2005,2(1):38-41.
[49]邵瑞莹等.高效液相-质谱法联用测定大鼠体内去甲基斑鳌素血药浓度.药物分析杂志,2004,22(1):607-609.
[50]廖文强,沈月华等.毛细管区带电泳方法对大鼠体内地西浮血药浓度的测定.中国医院药学杂志,2002,22(1):9-10.
[51]张国辉,马辰.黄酮类化合物药动学研究进展.中草药,2004,35(5):582-585.
[52]李应勤,陆蕴茹,魏璐雪.白射干黄酮类成分的研究[J].药学学报,1986,21(11):836-841.
[53]Li Jin,Hai Sheng Chen,Zhao Bao Xiang,et al.New flavone and isoflavone glycoside from Belamcanda chinensis[J].Chinese Chemical Letters,2007,18(2):158-160.
[54]PENG Xian,TAN Li.Studies on the constimnets from the the Rhazoma of Alisma orientalis[J]. Chinese Pharmaceutical Sciencs,1998(3):173-174.
[55]乔春峰,李秋文,董辉等.闭鞘姜属两种植物的化学成分研究[J].中国中药杂志,2002,27(2):123-125.
[56]冯卫生,郑晓珂,刘云宝等.石胆草中黄酮碳苷的分离与结构鉴定[J].药学学报,2004,39(2):110-115.
[57]赵秀娟,吴坤.芹菜素代谢动力学研究进展[J].中国公共卫生,2007,23(8):1020-1021.