应用海藻提取物ECLean治理富营养化水体的初步研究
摘要
本论文针对我国亟待解决的地表水体富营养化问题，利用引进的生物制剂——海藻提取物EClean及综合措施处理不同类型富营养水体，探讨EClean治理我国富营养水体的效果与机理，并摸索其适宜使用方法，为今后Eclean生物技术在我国自然水体治理领域中的推广和应用提供科学依据，为我国水体富营养化问题的解决提供一种新的技术方法和思路。
1．室内试验表明，海藻提取物EClean可使水体的总氮、总磷、化学需氧量、浊度降低，藻类细胞浓度显著下降，各项水质指标转化前期快于后期，尤其是前10天变化明显。对于北京动物园这种V类富营养化水体，以Eclean为100mg／L浓度较为适合。
2．采用海藻提取物EClean及综合措施对北京动物园猩猩馆水域进行了两年室外现场处理，结果表明：处理区与对照区相比水体的浊度和总悬浮物下降，水体的总氮、总磷和化学需氧量显著降低，藻类细胞密度减少；各项指标均反映出处理区水质好于动物园其它水域，处理水体的营养状态指数低于动物园内大多数水域的营养状态指数。
3．应用海藻提取物EClean及配套措施对锦绣大地高密度温室鱼塘水体进行处理，试验结果显示该技术能够改善养殖水体水质，改变鱼池浮游植物的优势种类，抑制蓝藻生长，减缓此类水体富营养化的进程，削减有害藻类对鱼类的不利影响，直接或间接的促进了鱼的正常生长发育。
4．经过海藻提取物EClean处理的水体，水中的氨化细菌、硝化细菌、亚硝化细菌的数量比对照明显增多，而且在曝气条件下促进了一定数量的反硝化细菌的生长，从而促进氮素循环和水中氮的释放，从机理上揭示了氮素降低的原因。
5．EClean对不同藻类的抑制性试验表明，EClean对蓝藻门的水华鱼腥藻(Anabaenaflos-aquae)、小颤藻(Oscillatoria tenuis)和绿藻门的小球藻(chlorella vulgaris)有明显的抑制作用，对绿藻门的斜生栅藻(Scenedesmus obliquus)和裸藻门的血红裸藻(Euglena sanguinea)的生长有一定的促进作用，显示出EClean对蓝藻性水华的治理具有重要意义。
The effect of the seaweed extract Eclean was studied in treating eutrophicated water. The purpose of the experiments is to find a way in dealing with the water eutrophication in China.
1. Indoor experiment of treating eutriphcated water with seaweed extract Eclean shows that the TN, TP & NTU were reduced and algae density was descended markedly than the control. Water quality changed apparently in the early days, especially in the early lOdays. The proper concentration of EClean application should be 100mg/L for the V class eutrophication water like the water in Beijing zoo.
2. The experiment in treating the eutrophicated water in Beijing Zoo with seaweed extract EClean and combined measures shows that, the water quality in treated area was improved evidently than the control, including Turbidity, TN, TP, COD, and density of algae.
3. The experiment in the eutrophicated fishpond in Jinxiudadi proved that the seaweed extract Eclean enhanced the capability of water self-purifying, improved water quality and restrained the growth of blue algae. It also made the fish more active in feeding compared with the fish in control pond.
4. The number of ammonifying bacteria, nitrosobacteria, and nitrobacteria got increased in the water treated with seaweed extract. There was also an increasing trend in the number of denitrifying bacteria in aerated-treatment water. This showed that the seaweed extract Eclean could accelerate nitrogen cycle and the release of nitrogen from water, which may be a reason for the lowering of nitrogen in water.
5. The experiments also showed that Eclean restrained the growth of some blue algae such as anabaena flos-aquae and Oscillatoria tennis and some green algae such as chlorella. vulgaris. This shows that the seaweed extract Eclean is very important and very useful in controlling the blue algae water bloom.
1．室内试验表明，海藻提取物EClean可使水体的总氮、总磷、化学需氧量、浊度降低，藻类细胞浓度显著下降，各项水质指标转化前期快于后期，尤其是前10天变化明显。对于北京动物园这种V类富营养化水体，以Eclean为100mg／L浓度较为适合。
2．采用海藻提取物EClean及综合措施对北京动物园猩猩馆水域进行了两年室外现场处理，结果表明：处理区与对照区相比水体的浊度和总悬浮物下降，水体的总氮、总磷和化学需氧量显著降低，藻类细胞密度减少；各项指标均反映出处理区水质好于动物园其它水域，处理水体的营养状态指数低于动物园内大多数水域的营养状态指数。
3．应用海藻提取物EClean及配套措施对锦绣大地高密度温室鱼塘水体进行处理，试验结果显示该技术能够改善养殖水体水质，改变鱼池浮游植物的优势种类，抑制蓝藻生长，减缓此类水体富营养化的进程，削减有害藻类对鱼类的不利影响，直接或间接的促进了鱼的正常生长发育。
4．经过海藻提取物EClean处理的水体，水中的氨化细菌、硝化细菌、亚硝化细菌的数量比对照明显增多，而且在曝气条件下促进了一定数量的反硝化细菌的生长，从而促进氮素循环和水中氮的释放，从机理上揭示了氮素降低的原因。
5．EClean对不同藻类的抑制性试验表明，EClean对蓝藻门的水华鱼腥藻(Anabaenaflos-aquae)、小颤藻(Oscillatoria tenuis)和绿藻门的小球藻(chlorella vulgaris)有明显的抑制作用，对绿藻门的斜生栅藻(Scenedesmus obliquus)和裸藻门的血红裸藻(Euglena sanguinea)的生长有一定的促进作用，显示出EClean对蓝藻性水华的治理具有重要意义。
The effect of the seaweed extract Eclean was studied in treating eutrophicated water. The purpose of the experiments is to find a way in dealing with the water eutrophication in China.
1. Indoor experiment of treating eutriphcated water with seaweed extract Eclean shows that the TN, TP & NTU were reduced and algae density was descended markedly than the control. Water quality changed apparently in the early days, especially in the early lOdays. The proper concentration of EClean application should be 100mg/L for the V class eutrophication water like the water in Beijing zoo.
2. The experiment in treating the eutrophicated water in Beijing Zoo with seaweed extract EClean and combined measures shows that, the water quality in treated area was improved evidently than the control, including Turbidity, TN, TP, COD, and density of algae.
3. The experiment in the eutrophicated fishpond in Jinxiudadi proved that the seaweed extract Eclean enhanced the capability of water self-purifying, improved water quality and restrained the growth of blue algae. It also made the fish more active in feeding compared with the fish in control pond.
4. The number of ammonifying bacteria, nitrosobacteria, and nitrobacteria got increased in the water treated with seaweed extract. There was also an increasing trend in the number of denitrifying bacteria in aerated-treatment water. This showed that the seaweed extract Eclean could accelerate nitrogen cycle and the release of nitrogen from water, which may be a reason for the lowering of nitrogen in water.
5. The experiments also showed that Eclean restrained the growth of some blue algae such as anabaena flos-aquae and Oscillatoria tennis and some green algae such as chlorella. vulgaris. This shows that the seaweed extract Eclean is very important and very useful in controlling the blue algae water bloom.
引文
1. Alken Murray Corp,USA. Aquaculture/lake/pond/river remediation, abstract so fcase studies. http://www.alken murray.com/aquastudies.html, 2001-11-02/2001-09-26
2. Benndorf J. Food web manipulation without nutrient control: A useful strategy in lake restoration? Schweiz Z Hydrol, 1987, 49:237-248
3. Buchan L., Possible biological mechanism of phosphorus removal, War. Sci. Tech., 1983,15(34): 87-103
4. Caiola M G, Pellegrini S, Lysis of Microcystis aeruginosa (KOLZ). by Bdellovibrio-like bacteria. J. Phycol., 1984, 20:471-475.
5. Carpenter S R, Kitchell J E Trophic cascade and biomanipulation: interface of research and management. A reply to the comment by DeMelo et al. Limnology and Oceanography, 1992, 37:208-213
6. Carvalhol, Beklioglu M, Moss B, 1995. Changes in a deep lake following sewage diversion-a challenge to the orthodoxy of external phosphorus control as a restoration strategy? Fresh water biology,34(2):399-410
7. Colwell F S, Speidel H K, Diffusion through a double-sided plate; development of a method to study algae-bacterium interactions. Appl. Environm. Microbiol., 1985, 50:1357-1360.
8. Comeau Y., Hall K J., Hankock R. E. W. and Oldham W. K., Bilolgical model for enhanced biological phosphorusre-moval. Wat. Res., 1986, 20:1511～1521
9. Cooke G D. Advanced treatment and diversion of wastewater storm water in lake and reservoir restoration. Butter worth Boston, MA, 1986
10. D.R. Woods and M.Sc., et al. Lea Marston purification lake: Operational and river-quality aspects. Water Pollution Control, 1998, 243
11. Daft M J, Sterwart W O P, Ecological studies on algal-lysing bacteria in fresh waters. Freshwat. Biol., 1957, 5:577-596.
12. Dakhama A, et al. Isolation and identification of antialgal substances produced by Pseudomonas aeruginosa. J. Appl. Phycol., 1993,5:295-306.
13. Dakhama A, et al. Stimulatory and inhibitory effects of Pseudomonas on the growth of algae. Can Tech. Rep. Fish. Aquat. Sci., 1989, 1714:46-51.
14. DeMelo R, France R, Me Queen D J. Biomanipulation: hitormyth. Limnology and Oceanography, 1992.37:192-207
15. Gitelman H J. Aluminium and Health: A Review, 1989
16. Graneli W, Doris Solander, Influe of aquatic macrophytes cycling in lakes. Hydrobiologia, 1988,170:245-266
17. Gromov B V, et al .A flexibacter that lyses blue-green algae. Microbiology. 1972,41:952-956.
18. Gumbricht T. Nutrient removal processes in freshwater submersed macrophyle systems.
Ecological Engineering, 1993, 2:1-30
19. Hiraishia A., Masamune K. and Kitamura H., Characterization of the bacterial population structure in an anaerobicaerobic activasted sludge system on the basis of respiratory quinone profiles, App. Micro., 55:897～901
20. Horppila J, Peltonen H, Malinen T.,et al. Top-down or bottom-up effects by fish: Issues of concern in biomanipulation of lake. Restoration Ecology,1998,6(1):20-28
21. Hosper S H. Stable states, buffers and swiches: an ecosysiem approach to the restoration and mangement of shallow lakes in Netherlands. Wat Sci Tech, 1998, 37(3): 151-164
22. Jacoby J M, Gibbons H L, Stoops K B. Response of a shallow, polymictic lake to buttered alum treatment. Lake Reservoir Manage, 1994, 10(2):103-112
23. Kairesalo T, Laine S, Luokkanen E, etal.1999.Direct and indirect mechanisms behind successful biomanipulation, Hydrobiologia,395/396:99-106
24. Kasprzak P, Krienitz L, Loschel R. Biomanip wlation: a limnological in lake ecotechnology of eutrophication management? Men Ist ltal idrobool, 1993,52:151-169.
25. Kuba T., van Loosdrecht M. C. M. and Heijnen J.J., Biological dephosphation by activated sludge under denitrifyin conditions: pH influence and occurrence of denitrifying dephosphation in a full-scale waster treatment plant, Wat. Sci. Tech., 199, 36:75～82
26. Kubra T. Wachtmeister A., van Loosdrecht M. C. M., Heijinen J.J. Effect of nitriate on phosphorus release in biological phosphorus removal systems, Wat. Sci. Tech., 1994, 30: 263～269
27. Mc Queen D J. Post J R. Mills E L. Trophic relationships in fresh water pelagic interactions. Verb Intern Verein Limnol, 1986, 23:739-747
28. Miller R G & Kopfler F C. The occurance of aluminium in drinking water. J. Water Works Assoc.,1984,76:84-91
29. Milligan K L D, Cosper E M. Isolation of virus capable of lysing the brown tide microalga, Aureococcus anophagefferens. Science., 1994, 266: 805-807.
30. Moss B,Stersfield J, Irvine K. Development of Daphnid communities in Diatom and Cyanophyte Dominated lakes and their Relevance to lake Restoration by Biomanipulation. J. Applied Ecology, 1991, 28:585-602
31. Muscutt A D, Harris G L, Baily S W, et al. Bufferzones to improve water qualirt: a review of their potential use in UK agriculture. Agriculture, Ecosystem and Environment, 1993,45: 59-77
32. Palph Mitchell. Water pollution microbiology (Vol.2)[M] Chichester, England: John Wiley & Sons Inc, 1978
33. Perow MR,MeijerM,Dawidowiczp,etal.Bionmanipulation in shallow lake: state of the art.Hydrobiologia,1997,342/343:355～365
34. PU Peimin, WANG Guoxiang, HU Chunhua, etal. Can we control the lake eutrophication by dredging. Eighth international Symposium on the Interactions between Sedimentsand Water.
September 13-17, 1999, Beijing
35. Reddy K R, DeBusk T A. State-of-the-art utilization of aquatic plant in water pollution control. Water Science Technology, 1987,19(10):61-79
36. Redhead K, Wright S J L. Isolation and properties of fungi that lyse blue-green algae. Applied Environment Microbiol., 1978,35:962-969
37. Reim R L, et al. The characterization of a Bacillus capable of blue-green bactericidal activity, Can, J. Microbiol. 1974, 20:981-986.
38. Richerson P.J.,et al. The causes and control of algal blooms in Clear Lake: Clean Lakes diagnostic feasibility study for Clear Lake, California. University of Califomia, Davis, 1994
39. Sand-Jensen K, Jeppese E, Nielsen, et al. Growth of macrophytes and ecosytem consequences in a lowland Danish stream. Freshwater Biology, 1989,22:15-32
40. Sas H. Lake restoration by reduction by reduction of nutrient loading: expectations, experiences, extrapolations. Academic Verlag Richare. St. Augustin, 1989
41. Satoh. Mino T. and Matsuo T., Uptake of organic substrates and accumulating of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic condition in the biological excess phosphate removalprocess, Wat. Sci. Tech., 1992, 26(5):933～942
42. Seip K L. Phoshporus and nitrogen limitation of algal biomass across trophic gradients. Aquatic Science, 1994, 56 (1):16-28
43. Shapiro J. Biomanipulation: The Next Phase Making it Stable. Hydrobiologia, 1990,200/201 : 13-27
44. Shapiro J. Lamarra V, Lynch M. Biomanipulation: an ecosystem approach to lake restoration. In: Brezonik D L, Fox J Leds. Water Quality Management through Biological Ways. Gainesville: University Press of Florida, 1975:85-96
45. Shilo M. Lysis of blue-green algae by myxobacter. J. Bacteriol., 1970,140:453-461.
46. Siebriz. I.P., Ekama G. and Marais G.v.R., A parametric model for biological excess phosphorus removal. War. Sci. Tech., 1983,15(34): 127～152
47. Sieburth J M. et al. Ultrastructure and ecology of Aueococcus anophagefferens Gen. et sp. Nov. (Chrysphyccac): the dominant picoplankton during a bloom in Narragansett Bay, Rhode Island, summer 1985. J. Phycol., 24, 416-425.
48. Sparrow F K. Two new species of Pythium parasitic in green algae. Ann. Bot. (London), 1971,45:257-277.
49. Suttle C A, et al. Infection of phytoplankton by viruses and reduction of primary productivity. Nature., 1990, 347:467-469.
50. Tilley D R, Brown M T. Wetland net works for storm water management in subtropical urban watersheds. Ecological Engineering, 1996,10(2):226
51. U K Environmental Agency. Environmental ISSUES series aquatic eutrophication in England and Wales[R]. U K Environmental Agency Consultative Report, December 1998
52. Van Liere L, Parma S & Gulati R D. Working group water quality research Loosdreht Lakes: its history, structure, research program me, and some results, Hydrobiologia, 1992,233:1-9
53. Vander Does J, Verstraelen P, Boers P, etal. Lake restoration with and without dredging of phosphorus-enriched upper sediment layers. Hydrobiologia, 1992,233:197-210
54. Vollenweider R A. OECD Technical Report DAS/CSI/68.27, Paris,OECD. 1968.159pp
55. Welch E B,Barbiero R P, Bouchard D, et al. Lake trophic state change and constant algal composition following dilution and diversion. Ecological Engineerion, 1992,1 : 173-197
56. Wetzel R G. Factors influe ncing photosyn thisis and excretion of dissolved organic matter by aquaic macrophytes in hard water lakes. Verh Int Verein Limnol, 1969, 17:72-85
57. WU R S S. Eutrophication, water borne pathogens and xenobiotic compounds: environmental risks and challenges. Marine pollution bulletin, 1999, 39(1): 11-22
58．北京城市节水办公室，北京实现水资源可持续利用的对策，能源保持与环境保护．2002，3
59．边归国，湖泊和水库中藻类去除方法的研究进展．福建环境，2003，20(5)：62-65
60．陈绍明，郑福寿．水生生物学实验法．北京：海洋出版社．1985．81-86，227-241
61．陈水勇，吴振明，水体富营养化的形成、危害和防治．环境科学与技术，1999
62．陈秀为，张克强，牛成玉．光合细菌在模拟试验中净化水质作用的研究．农业环境保护，1995，14(3)：135-136
63．邓如福，裴炎，王瑜宁等．海藻糖对水稻幼苗抗寒性研究．西南农业大学学报，1991，13(3)：347-349
64．《地表水环境质量标准基本项目标准限制》GB3838-2002
65．丁彦文，艾红．微生物在水产养殖中的应用．湛江海洋大学学报，2003，20(1)：68-73
66．东野脉兴，樊竹青，张灼，夏学惠，田升平，周建民．云南滇池微生物对磷循环与沉积作用的实验研究．化工矿产地质2003(6)：65-76
67．杜桂森，孟繁艳，李学东，张为华，王建厅，武殿伟．密云水库水质现状及发展趋势．环境科学．1999,Vol．20(2)：110-113
68．杜桂森，王建厅，张为华．北京河湖的营养状态分析，北京水利．2002(6)：25-28
69．范晓、韩丽君．我国常见海藻的营养分析．中国海洋药物．1993(4)：32
70．顾宗濂．中国富营养化湖泊的生物修复．农村生态环境，2002，18(1)：42-45
71．顾祖宜．应用光合细菌处理有机废水的研究[J]．中国环境科学，1985，5(2)：29-33
72．郭慧光，马丕京．滇池环境综合治理框架及其投资估算．云南环境科学，2000，19(增)：106-113
73．国家环境保护总局编委会．水和废水监测分析方法(第四版)．北京：中国环境科学出版社，2002．
74．韩丽君，周天成．海藻植物生长调节剂中的活性组分．海洋科学，1999，5：21-22
75．胡先文，董元彦，张新萍，叶发兵．可见分光光度法测定水华鱼腥藻．华中农业大学学报，2002，21(3)，295-297
76．胡先文，张新萍，董元彦等．甲磺隆对水华鱼腥藻的抑制效应．农药，2003，42(12)：39-40
77．黄鹄，陈卓如，王丹青．城市缺水问题的对策探讨．环境保护，2001，11：14-15
78．黄文钰，吴延根，舒金华，中国主要湖泊水库的水环境问题与防治建议．湖泊科学，1998，10(3)
79．季民，卢燕玲，庞金钊，郝建东，薛广宁．人工细菌对水体污染净化效果的实验研究，天津城市建设学院学报 2002，Vol．8 No．1，1-4
80．蒋道松，刘其城，章俭，尉苏娟．除藻技术新进展．常德师范学院学报(自然科学版)，2000，12(1)：25-31
81．金送笛，李永函，倪彩虹等．菹草(Potamogeton crispus)对水中氮磷的吸收及若干影响因素．生态学报，1994，14(2)：168-173
82．金相灿，屠清瑛主编，湖泊富营养化调查规范．第二版．北京：中国环境科学出版社，1990
83．金相灿等，湖泊富营养化控制和管理技术．北京：化学工业出版社，2001
84．金相灿等，中国湖泊富营养化调查规范(第一版)．北京：中国环境科学出版社，1987
85．柯清水．硝化细菌与水产养殖的关系如何．养鱼世界，1999(1)：83～86
86．李春选．海洋天然药物—抗爱滋病活性物质的来源．中国海洋药物，1993(3)：22
87．李春雁，崔毅．生物操纵法对养殖水体富营养化防治的探讨．2002，23(1)海洋水产研究，71-75
88．李维炯，倪永珍．EM(有效微生物群)的研究与应用．生态学杂志，1995，14(5)：58-62
89．李雪梅，杨中艺，简曙光，等．有效微生物群控制富营养化湖泊蓝藻的效应．中山大学学报，2000，39(1)：81-85
90．李正魁，濮培民．固定化增殖氮循环细菌群S B R法净化富营养化湖水．核技术，2001(24)：8，674-679
91．廖日红，井艳文．北京城区河湖水体水华现象成因的初步分析，北京水利，2002(5)：32-35
92．刘鸿志，卢雪云．禁磷不能“一刀切”．中国环境报，2001-07-27
93．刘鸿志．中国太湖和日本琵琶湖水污染防治状况比较．中国环境报，2001-07-13
94．刘淑媛，任久长，由文辉．利用人工基质无土栽培经济植物净化富营养化水体的研究．北京大学学报，1999，35(4)：1-5
95．刘文祥．人工湿地在农业污染面源污染控制的应用研究．环境科学研究，1997，10(4)：15-19
96．柳建生，杨清凉．水体富营养化检测评估与防治．1989，北京：环境科学出版社
97．楼春华，廖日红，侯德，龚应安，孟庆义．北京城区河湖富营养化特征及对策分析．北京水利．2003(6)：18-21
98．吕军仪，陈志胜，杨大伟，等，广东省粤东地区工厂化高密度养鲍业病害情况调查报告．水产科技．1999，(4)：1-4．
99．吕琴，陈声明．光合细菌在有机废水处理中的应用．食品与发酵工业，1995，6：66
100．马登军，张凤娥，高云霞，陈沾，张艳敏．官厅水库富营养的评价．中国环境监测．2002，18(1)：41-44
101．马立珊，骆永明，吴文华，等．浮床香根草对富营养化水体氮磷去除动态及效率的初步研究[J]土壤2002(2)：99-101
102．马世骏．生态工程—生态系统原理的应用．生态学杂志，1983(4)：20—22
103．马文漪，杨柳燕．环境微生物工程[M]．南京：南京大学出版社，1998：175-196
104．孟庆义，国内湖泊水质污染及富营养化治理，北京水利，2001(5)：45-49
105．濮培民，胡维平，王国祥等．太湖环境治理．见：蔡启铭主编．太湖环境生态研究．北京：气象出版社，1998：188-201
106．濮培民，王国祥，胡春华，等．底泥疏浚能控制湖泊富营养化吗．湖泊科学，2000，12(3)：269-279[9]
107．钱薇薇，朱鑫华．海水养殖业的发展及前景．中国水产，2000(2)：18-19
108．任翱，史家梁．应用微生物技术改善育鳖池水质的实验研究．农村生态环境2001，17(1)：48-51
109．史家梁，朱核光．粪便污水的光合细菌液肥化及其应用研究．应用与环境生物学报，1995，1(2)：188
110．史家梁．光合细菌(PSB)与日本的水产养殖．水产科技情报，1995，22(5)：212-216
111．舒金华，黄文钰，高锡芸等．发达国家禁用(限用)含磷洗衣粉的措施．湖泊科学，1998，10(1)：90-96
112．宋秀杰，郑希伟．北京市生态环境现状及生态保护发展战略探讨．环境保护2001(1)：30-32
113．孙克年．海藻饲料资源在水产养殖中的开发应用．广东饲料，2001(4)：58-69
114．孙铁珩，周启星，李培军．污染生态学．北京：科学出版社，2002，160-166
115．孙文浩，俞子文，余叔文．城市富营养化水域的生物治理和凤眼莲抑制藻类生长机理．环境科学学报，1989，9(2)：188—195
116．孙文浩．凤眼莲根系分泌物中的克藻化合物．植物生理学报，1993
117．汤琳，袁峻峰，陈德辉．十二烷基苯磺酸钠对于几种藻类的毒性试验．上海师范大学学报(自然科学版)，2000，29(2)：70-74
118．万育生，靳顶．北京及上游周边地区水资源问题对策研究．水资源保护．2002(1)：11-16
119．万育生，靳顶．北京及上游周边地区水资源问题对策研究．水资源保护．2002(1)：11-16
120．王朝晖，江天久，杞桑．水网藻(Hydrodictyon reticulatum)对富营养化水样中氮磷去除能力的研究．环境科学学报，1999(7)：448-453
121．王福表．网箱养殖水污染及治理对策．2002，26(7)，海洋科学，24-26
122．王国祥，濮培民．若干人工调控措施对富营养化湖泊藻类种群的影响．环境科学，1999，20(2)：71-74
123．王三根，邓如福，裴炎，等．海藻糖提高绵阳15号小麦幼苗耐盐能力的研究．西南农业大学学报，1992，14(2)：182-185
124．王卫平．食品品质改良剂：亲水胶体的性质及应用—海藻胶．食品与发酵工业，1996(1)：72
125．王肇鼎，彭云辉，孙丽华，陈沽如．大鹏澳网箱养鱼水体自身污染及富营养化研究．2003，27(2)，海洋科学，77-81
126．魏开金．光合细菌及其在水产养殖上的应用．淡水渔业，1991，2：41
127．温志良．海水养殖对环境的影响．南海研究与开发，2000，(1)：37-41
128．翁稣颖等．环境微生物学．科学出版社．1985，134-146
129．香山科学会议．我国湖泊富营养化的发生机制与控制对策．地球科学进展，2004(2)
130．徐秀丽，范晓，宋福行．中国经济海藻提取物生物活性．海洋与湖沼，2004，35(1)：55-64
131．杨维本，高效复合微生物菌群处理城市污水的初步研究：[硕士学位论文]．南京：南京工业大学，2002
132．于明，周云龙．从浮游植物的变化看光合细菌在治理富营养化水体中的作用．北京师范大学学报(自然科学版)．2001，37(5)：679-684
133．余冉，昌锡武，费治文．富营养化水体藻类和藻毒素处理研究．环境导报，2002(4)：14-16
134．袁旭音，中国湖泊污染状况的基本评价．火山地质与矿产，2000，21(2)：128-136
135．曾宇，秦松．光合细菌法在水处理中的应用．城市环境与城市生态，2000，19(1)：38-39
136．张丽萍，袁文权，张锡辉．底泥污染物释放动力学研究．环境污染治理技术与设备，2003(2)：22—26
137．张锡辉．水环境修复工程学原理与应用．北京：化学工业出版社，2002
138．张以勤．从《本草纲目》收载的谈海药的历史及现代应用．中国中药杂志，1993(8)：505
139．赵以军，刘永定．有害藻类及其微生物防治的基础——藻菌关系的研究动态．水生生物学报，1996，20(2)：173-181
140．赵章元．用综合除藻法治理滇池蓝藻污染．环境保护，2000，(5)：12-14
141．中华人民共和国水利部，中国水资源公报，1997-2001年
142．周宏．杭州西湖水体中叶绿素a含量与水质的关系．浙江大学学报(理学版)，2001，28(4)：439：443
143．周歧存，肖风波．海藻在南美白对虾饲料中的应用研究．海洋科学，2003，27(3)：66-69
144．周群英，高廷耀．环境工程微生物学，北京：高等教育出版社，2000：197-204
145．周少奇，周吉林．生物脱氮新技术研究进展．环境污染治理技术与设备，2000(12)：11-18
2. Benndorf J. Food web manipulation without nutrient control: A useful strategy in lake restoration? Schweiz Z Hydrol, 1987, 49:237-248
3. Buchan L., Possible biological mechanism of phosphorus removal, War. Sci. Tech., 1983,15(34): 87-103
4. Caiola M G, Pellegrini S, Lysis of Microcystis aeruginosa (KOLZ). by Bdellovibrio-like bacteria. J. Phycol., 1984, 20:471-475.
5. Carpenter S R, Kitchell J E Trophic cascade and biomanipulation: interface of research and management. A reply to the comment by DeMelo et al. Limnology and Oceanography, 1992, 37:208-213
6. Carvalhol, Beklioglu M, Moss B, 1995. Changes in a deep lake following sewage diversion-a challenge to the orthodoxy of external phosphorus control as a restoration strategy? Fresh water biology,34(2):399-410
7. Colwell F S, Speidel H K, Diffusion through a double-sided plate; development of a method to study algae-bacterium interactions. Appl. Environm. Microbiol., 1985, 50:1357-1360.
8. Comeau Y., Hall K J., Hankock R. E. W. and Oldham W. K., Bilolgical model for enhanced biological phosphorusre-moval. Wat. Res., 1986, 20:1511～1521
9. Cooke G D. Advanced treatment and diversion of wastewater storm water in lake and reservoir restoration. Butter worth Boston, MA, 1986
10. D.R. Woods and M.Sc., et al. Lea Marston purification lake: Operational and river-quality aspects. Water Pollution Control, 1998, 243
11. Daft M J, Sterwart W O P, Ecological studies on algal-lysing bacteria in fresh waters. Freshwat. Biol., 1957, 5:577-596.
12. Dakhama A, et al. Isolation and identification of antialgal substances produced by Pseudomonas aeruginosa. J. Appl. Phycol., 1993,5:295-306.
13. Dakhama A, et al. Stimulatory and inhibitory effects of Pseudomonas on the growth of algae. Can Tech. Rep. Fish. Aquat. Sci., 1989, 1714:46-51.
14. DeMelo R, France R, Me Queen D J. Biomanipulation: hitormyth. Limnology and Oceanography, 1992.37:192-207
15. Gitelman H J. Aluminium and Health: A Review, 1989
16. Graneli W, Doris Solander, Influe of aquatic macrophytes cycling in lakes. Hydrobiologia, 1988,170:245-266
17. Gromov B V, et al .A flexibacter that lyses blue-green algae. Microbiology. 1972,41:952-956.
18. Gumbricht T. Nutrient removal processes in freshwater submersed macrophyle systems.
Ecological Engineering, 1993, 2:1-30
19. Hiraishia A., Masamune K. and Kitamura H., Characterization of the bacterial population structure in an anaerobicaerobic activasted sludge system on the basis of respiratory quinone profiles, App. Micro., 55:897～901
20. Horppila J, Peltonen H, Malinen T.,et al. Top-down or bottom-up effects by fish: Issues of concern in biomanipulation of lake. Restoration Ecology,1998,6(1):20-28
21. Hosper S H. Stable states, buffers and swiches: an ecosysiem approach to the restoration and mangement of shallow lakes in Netherlands. Wat Sci Tech, 1998, 37(3): 151-164
22. Jacoby J M, Gibbons H L, Stoops K B. Response of a shallow, polymictic lake to buttered alum treatment. Lake Reservoir Manage, 1994, 10(2):103-112
23. Kairesalo T, Laine S, Luokkanen E, etal.1999.Direct and indirect mechanisms behind successful biomanipulation, Hydrobiologia,395/396:99-106
24. Kasprzak P, Krienitz L, Loschel R. Biomanip wlation: a limnological in lake ecotechnology of eutrophication management? Men Ist ltal idrobool, 1993,52:151-169.
25. Kuba T., van Loosdrecht M. C. M. and Heijnen J.J., Biological dephosphation by activated sludge under denitrifyin conditions: pH influence and occurrence of denitrifying dephosphation in a full-scale waster treatment plant, Wat. Sci. Tech., 199, 36:75～82
26. Kubra T. Wachtmeister A., van Loosdrecht M. C. M., Heijinen J.J. Effect of nitriate on phosphorus release in biological phosphorus removal systems, Wat. Sci. Tech., 1994, 30: 263～269
27. Mc Queen D J. Post J R. Mills E L. Trophic relationships in fresh water pelagic interactions. Verb Intern Verein Limnol, 1986, 23:739-747
28. Miller R G & Kopfler F C. The occurance of aluminium in drinking water. J. Water Works Assoc.,1984,76:84-91
29. Milligan K L D, Cosper E M. Isolation of virus capable of lysing the brown tide microalga, Aureococcus anophagefferens. Science., 1994, 266: 805-807.
30. Moss B,Stersfield J, Irvine K. Development of Daphnid communities in Diatom and Cyanophyte Dominated lakes and their Relevance to lake Restoration by Biomanipulation. J. Applied Ecology, 1991, 28:585-602
31. Muscutt A D, Harris G L, Baily S W, et al. Bufferzones to improve water qualirt: a review of their potential use in UK agriculture. Agriculture, Ecosystem and Environment, 1993,45: 59-77
32. Palph Mitchell. Water pollution microbiology (Vol.2)[M] Chichester, England: John Wiley & Sons Inc, 1978
33. Perow MR,MeijerM,Dawidowiczp,etal.Bionmanipulation in shallow lake: state of the art.Hydrobiologia,1997,342/343:355～365
34. PU Peimin, WANG Guoxiang, HU Chunhua, etal. Can we control the lake eutrophication by dredging. Eighth international Symposium on the Interactions between Sedimentsand Water.
September 13-17, 1999, Beijing
35. Reddy K R, DeBusk T A. State-of-the-art utilization of aquatic plant in water pollution control. Water Science Technology, 1987,19(10):61-79
36. Redhead K, Wright S J L. Isolation and properties of fungi that lyse blue-green algae. Applied Environment Microbiol., 1978,35:962-969
37. Reim R L, et al. The characterization of a Bacillus capable of blue-green bactericidal activity, Can, J. Microbiol. 1974, 20:981-986.
38. Richerson P.J.,et al. The causes and control of algal blooms in Clear Lake: Clean Lakes diagnostic feasibility study for Clear Lake, California. University of Califomia, Davis, 1994
39. Sand-Jensen K, Jeppese E, Nielsen, et al. Growth of macrophytes and ecosytem consequences in a lowland Danish stream. Freshwater Biology, 1989,22:15-32
40. Sas H. Lake restoration by reduction by reduction of nutrient loading: expectations, experiences, extrapolations. Academic Verlag Richare. St. Augustin, 1989
41. Satoh. Mino T. and Matsuo T., Uptake of organic substrates and accumulating of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic condition in the biological excess phosphate removalprocess, Wat. Sci. Tech., 1992, 26(5):933～942
42. Seip K L. Phoshporus and nitrogen limitation of algal biomass across trophic gradients. Aquatic Science, 1994, 56 (1):16-28
43. Shapiro J. Biomanipulation: The Next Phase Making it Stable. Hydrobiologia, 1990,200/201 : 13-27
44. Shapiro J. Lamarra V, Lynch M. Biomanipulation: an ecosystem approach to lake restoration. In: Brezonik D L, Fox J Leds. Water Quality Management through Biological Ways. Gainesville: University Press of Florida, 1975:85-96
45. Shilo M. Lysis of blue-green algae by myxobacter. J. Bacteriol., 1970,140:453-461.
46. Siebriz. I.P., Ekama G. and Marais G.v.R., A parametric model for biological excess phosphorus removal. War. Sci. Tech., 1983,15(34): 127～152
47. Sieburth J M. et al. Ultrastructure and ecology of Aueococcus anophagefferens Gen. et sp. Nov. (Chrysphyccac): the dominant picoplankton during a bloom in Narragansett Bay, Rhode Island, summer 1985. J. Phycol., 24, 416-425.
48. Sparrow F K. Two new species of Pythium parasitic in green algae. Ann. Bot. (London), 1971,45:257-277.
49. Suttle C A, et al. Infection of phytoplankton by viruses and reduction of primary productivity. Nature., 1990, 347:467-469.
50. Tilley D R, Brown M T. Wetland net works for storm water management in subtropical urban watersheds. Ecological Engineering, 1996,10(2):226
51. U K Environmental Agency. Environmental ISSUES series aquatic eutrophication in England and Wales[R]. U K Environmental Agency Consultative Report, December 1998
52. Van Liere L, Parma S & Gulati R D. Working group water quality research Loosdreht Lakes: its history, structure, research program me, and some results, Hydrobiologia, 1992,233:1-9
53. Vander Does J, Verstraelen P, Boers P, etal. Lake restoration with and without dredging of phosphorus-enriched upper sediment layers. Hydrobiologia, 1992,233:197-210
54. Vollenweider R A. OECD Technical Report DAS/CSI/68.27, Paris,OECD. 1968.159pp
55. Welch E B,Barbiero R P, Bouchard D, et al. Lake trophic state change and constant algal composition following dilution and diversion. Ecological Engineerion, 1992,1 : 173-197
56. Wetzel R G. Factors influe ncing photosyn thisis and excretion of dissolved organic matter by aquaic macrophytes in hard water lakes. Verh Int Verein Limnol, 1969, 17:72-85
57. WU R S S. Eutrophication, water borne pathogens and xenobiotic compounds: environmental risks and challenges. Marine pollution bulletin, 1999, 39(1): 11-22
58．北京城市节水办公室，北京实现水资源可持续利用的对策，能源保持与环境保护．2002，3
59．边归国，湖泊和水库中藻类去除方法的研究进展．福建环境，2003，20(5)：62-65
60．陈绍明，郑福寿．水生生物学实验法．北京：海洋出版社．1985．81-86，227-241
61．陈水勇，吴振明，水体富营养化的形成、危害和防治．环境科学与技术，1999
62．陈秀为，张克强，牛成玉．光合细菌在模拟试验中净化水质作用的研究．农业环境保护，1995，14(3)：135-136
63．邓如福，裴炎，王瑜宁等．海藻糖对水稻幼苗抗寒性研究．西南农业大学学报，1991，13(3)：347-349
64．《地表水环境质量标准基本项目标准限制》GB3838-2002
65．丁彦文，艾红．微生物在水产养殖中的应用．湛江海洋大学学报，2003，20(1)：68-73
66．东野脉兴，樊竹青，张灼，夏学惠，田升平，周建民．云南滇池微生物对磷循环与沉积作用的实验研究．化工矿产地质2003(6)：65-76
67．杜桂森，孟繁艳，李学东，张为华，王建厅，武殿伟．密云水库水质现状及发展趋势．环境科学．1999,Vol．20(2)：110-113
68．杜桂森，王建厅，张为华．北京河湖的营养状态分析，北京水利．2002(6)：25-28
69．范晓、韩丽君．我国常见海藻的营养分析．中国海洋药物．1993(4)：32
70．顾宗濂．中国富营养化湖泊的生物修复．农村生态环境，2002，18(1)：42-45
71．顾祖宜．应用光合细菌处理有机废水的研究[J]．中国环境科学，1985，5(2)：29-33
72．郭慧光，马丕京．滇池环境综合治理框架及其投资估算．云南环境科学，2000，19(增)：106-113
73．国家环境保护总局编委会．水和废水监测分析方法(第四版)．北京：中国环境科学出版社，2002．
74．韩丽君，周天成．海藻植物生长调节剂中的活性组分．海洋科学，1999，5：21-22
75．胡先文，董元彦，张新萍，叶发兵．可见分光光度法测定水华鱼腥藻．华中农业大学学报，2002，21(3)，295-297
76．胡先文，张新萍，董元彦等．甲磺隆对水华鱼腥藻的抑制效应．农药，2003，42(12)：39-40
77．黄鹄，陈卓如，王丹青．城市缺水问题的对策探讨．环境保护，2001，11：14-15
78．黄文钰，吴延根，舒金华，中国主要湖泊水库的水环境问题与防治建议．湖泊科学，1998，10(3)
79．季民，卢燕玲，庞金钊，郝建东，薛广宁．人工细菌对水体污染净化效果的实验研究，天津城市建设学院学报 2002，Vol．8 No．1，1-4
80．蒋道松，刘其城，章俭，尉苏娟．除藻技术新进展．常德师范学院学报(自然科学版)，2000，12(1)：25-31
81．金送笛，李永函，倪彩虹等．菹草(Potamogeton crispus)对水中氮磷的吸收及若干影响因素．生态学报，1994，14(2)：168-173
82．金相灿，屠清瑛主编，湖泊富营养化调查规范．第二版．北京：中国环境科学出版社，1990
83．金相灿等，湖泊富营养化控制和管理技术．北京：化学工业出版社，2001
84．金相灿等，中国湖泊富营养化调查规范(第一版)．北京：中国环境科学出版社，1987
85．柯清水．硝化细菌与水产养殖的关系如何．养鱼世界，1999(1)：83～86
86．李春选．海洋天然药物—抗爱滋病活性物质的来源．中国海洋药物，1993(3)：22
87．李春雁，崔毅．生物操纵法对养殖水体富营养化防治的探讨．2002，23(1)海洋水产研究，71-75
88．李维炯，倪永珍．EM(有效微生物群)的研究与应用．生态学杂志，1995，14(5)：58-62
89．李雪梅，杨中艺，简曙光，等．有效微生物群控制富营养化湖泊蓝藻的效应．中山大学学报，2000，39(1)：81-85
90．李正魁，濮培民．固定化增殖氮循环细菌群S B R法净化富营养化湖水．核技术，2001(24)：8，674-679
91．廖日红，井艳文．北京城区河湖水体水华现象成因的初步分析，北京水利，2002(5)：32-35
92．刘鸿志，卢雪云．禁磷不能“一刀切”．中国环境报，2001-07-27
93．刘鸿志．中国太湖和日本琵琶湖水污染防治状况比较．中国环境报，2001-07-13
94．刘淑媛，任久长，由文辉．利用人工基质无土栽培经济植物净化富营养化水体的研究．北京大学学报，1999，35(4)：1-5
95．刘文祥．人工湿地在农业污染面源污染控制的应用研究．环境科学研究，1997，10(4)：15-19
96．柳建生，杨清凉．水体富营养化检测评估与防治．1989，北京：环境科学出版社
97．楼春华，廖日红，侯德，龚应安，孟庆义．北京城区河湖富营养化特征及对策分析．北京水利．2003(6)：18-21
98．吕军仪，陈志胜，杨大伟，等，广东省粤东地区工厂化高密度养鲍业病害情况调查报告．水产科技．1999，(4)：1-4．
99．吕琴，陈声明．光合细菌在有机废水处理中的应用．食品与发酵工业，1995，6：66
100．马登军，张凤娥，高云霞，陈沾，张艳敏．官厅水库富营养的评价．中国环境监测．2002，18(1)：41-44
101．马立珊，骆永明，吴文华，等．浮床香根草对富营养化水体氮磷去除动态及效率的初步研究[J]土壤2002(2)：99-101
102．马世骏．生态工程—生态系统原理的应用．生态学杂志，1983(4)：20—22
103．马文漪，杨柳燕．环境微生物工程[M]．南京：南京大学出版社，1998：175-196
104．孟庆义，国内湖泊水质污染及富营养化治理，北京水利，2001(5)：45-49
105．濮培民，胡维平，王国祥等．太湖环境治理．见：蔡启铭主编．太湖环境生态研究．北京：气象出版社，1998：188-201
106．濮培民，王国祥，胡春华，等．底泥疏浚能控制湖泊富营养化吗．湖泊科学，2000，12(3)：269-279[9]
107．钱薇薇，朱鑫华．海水养殖业的发展及前景．中国水产，2000(2)：18-19
108．任翱，史家梁．应用微生物技术改善育鳖池水质的实验研究．农村生态环境2001，17(1)：48-51
109．史家梁，朱核光．粪便污水的光合细菌液肥化及其应用研究．应用与环境生物学报，1995，1(2)：188
110．史家梁．光合细菌(PSB)与日本的水产养殖．水产科技情报，1995，22(5)：212-216
111．舒金华，黄文钰，高锡芸等．发达国家禁用(限用)含磷洗衣粉的措施．湖泊科学，1998，10(1)：90-96
112．宋秀杰，郑希伟．北京市生态环境现状及生态保护发展战略探讨．环境保护2001(1)：30-32
113．孙克年．海藻饲料资源在水产养殖中的开发应用．广东饲料，2001(4)：58-69
114．孙铁珩，周启星，李培军．污染生态学．北京：科学出版社，2002，160-166
115．孙文浩，俞子文，余叔文．城市富营养化水域的生物治理和凤眼莲抑制藻类生长机理．环境科学学报，1989，9(2)：188—195
116．孙文浩．凤眼莲根系分泌物中的克藻化合物．植物生理学报，1993
117．汤琳，袁峻峰，陈德辉．十二烷基苯磺酸钠对于几种藻类的毒性试验．上海师范大学学报(自然科学版)，2000，29(2)：70-74
118．万育生，靳顶．北京及上游周边地区水资源问题对策研究．水资源保护．2002(1)：11-16
119．万育生，靳顶．北京及上游周边地区水资源问题对策研究．水资源保护．2002(1)：11-16
120．王朝晖，江天久，杞桑．水网藻(Hydrodictyon reticulatum)对富营养化水样中氮磷去除能力的研究．环境科学学报，1999(7)：448-453
121．王福表．网箱养殖水污染及治理对策．2002，26(7)，海洋科学，24-26
122．王国祥，濮培民．若干人工调控措施对富营养化湖泊藻类种群的影响．环境科学，1999，20(2)：71-74
123．王三根，邓如福，裴炎，等．海藻糖提高绵阳15号小麦幼苗耐盐能力的研究．西南农业大学学报，1992，14(2)：182-185
124．王卫平．食品品质改良剂：亲水胶体的性质及应用—海藻胶．食品与发酵工业，1996(1)：72
125．王肇鼎，彭云辉，孙丽华，陈沽如．大鹏澳网箱养鱼水体自身污染及富营养化研究．2003，27(2)，海洋科学，77-81
126．魏开金．光合细菌及其在水产养殖上的应用．淡水渔业，1991，2：41
127．温志良．海水养殖对环境的影响．南海研究与开发，2000，(1)：37-41
128．翁稣颖等．环境微生物学．科学出版社．1985，134-146
129．香山科学会议．我国湖泊富营养化的发生机制与控制对策．地球科学进展，2004(2)
130．徐秀丽，范晓，宋福行．中国经济海藻提取物生物活性．海洋与湖沼，2004，35(1)：55-64
131．杨维本，高效复合微生物菌群处理城市污水的初步研究：[硕士学位论文]．南京：南京工业大学，2002
132．于明，周云龙．从浮游植物的变化看光合细菌在治理富营养化水体中的作用．北京师范大学学报(自然科学版)．2001，37(5)：679-684
133．余冉，昌锡武，费治文．富营养化水体藻类和藻毒素处理研究．环境导报，2002(4)：14-16
134．袁旭音，中国湖泊污染状况的基本评价．火山地质与矿产，2000，21(2)：128-136
135．曾宇，秦松．光合细菌法在水处理中的应用．城市环境与城市生态，2000，19(1)：38-39
136．张丽萍，袁文权，张锡辉．底泥污染物释放动力学研究．环境污染治理技术与设备，2003(2)：22—26
137．张锡辉．水环境修复工程学原理与应用．北京：化学工业出版社，2002
138．张以勤．从《本草纲目》收载的谈海药的历史及现代应用．中国中药杂志，1993(8)：505
139．赵以军，刘永定．有害藻类及其微生物防治的基础——藻菌关系的研究动态．水生生物学报，1996，20(2)：173-181
140．赵章元．用综合除藻法治理滇池蓝藻污染．环境保护，2000，(5)：12-14
141．中华人民共和国水利部，中国水资源公报，1997-2001年
142．周宏．杭州西湖水体中叶绿素a含量与水质的关系．浙江大学学报(理学版)，2001，28(4)：439：443
143．周歧存，肖风波．海藻在南美白对虾饲料中的应用研究．海洋科学，2003，27(3)：66-69
144．周群英，高廷耀．环境工程微生物学，北京：高等教育出版社，2000：197-204
145．周少奇，周吉林．生物脱氮新技术研究进展．环境污染治理技术与设备，2000(12)：11-18