光合细菌及活性污泥联合处理高浓度苯胺废水的实验研究
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摘要
苯胺废水的处置,一直以来都以物理化学方法为主,处理规模和效果上往往不够理想。传统活性污泥法及其改进方法,其对苯胺废水的处理不仅效果有限,往往还要经过较长时间的驯化,才能使活性污泥微生物适应苯胺废水的水质特性,耐负荷冲击效果亦不佳。近年来培养针对苯胺降解的专性微生物也是苯胺废水处理的一个重要研究方向,经过专门诱变、筛选、培养的专性微生物具有对苯胺废水适应性好,处理效率高等优点,但其运行成本往往不低,专性微生物的筛选和培养也需要大量的金钱和时间投入。
光合细菌(Photosynthetic Bacteria, PSB)由于其对高浓度有机废水的较好的耐受性,耐冲击能力等优点,近年来在水处理领域的应用日渐广泛。本文研究了实验室条件下,光合细菌及活性污泥法联用处理苯胺类高浓度有机废水的可行性及最佳处理工艺,并通过温度调整,抑制实验中藻类的大量繁殖。
研究结果表明：
经过PSB工艺段处理后的苯胺废水,其COD(用重铬酸钾氧化水中需氧污染物质时所消耗的氧气量)可由2304mg/L降至144-166mg/L,去除率可达92.8%。苯胺含量可由28.4mg/L降至2.85mg/L,去除率为90%。PSB段出水经过活性污泥反应器处理后,COD可降至84-100mg/L,苯胺浓度可降至0.71mg/L,整个工艺总的COD和苯胺去除率分别为96.4%和97.5%,达到国家一级排放标准。
光合细菌与生活污水中微生物可形成复合菌群,不仅增加了该废水的可生化性,也为活性污泥微生物的产生提供了丰富的营养条件,同时光合细菌还可保持优势种群。光合细菌自身的一些特点决定了它在进行一段时间的生长繁殖之后,其优势性会逐渐衰退,进而影响对废水的处理效果,为了解决这个问题,必须定期对反应器内投加新鲜菌液,且光合细菌菌液的扩大培养不能超过3次。
在PSB反应器中,每周期的厌氧时间可达44小时,变相延长了厌氧酸化状态时间,深化了苯胺类物质的酸化水解,保证了后续工艺中光合细菌及活性污泥微生物对苯胺及COD的去除率。
针对PSB反应器中出现的藻类异常繁殖现象,由于光合细菌对温度的耐受范围比藻类大,故用温度控制藻类生长。保证光合细菌处理苯胺类废水的处理效果。当反应器内温度控制在27The treatments of aniline wastewater always are physico-chemical method by primarily, but the treatment scale and effect are both not very good. The traditional activated sludge method and its modified methods are both not have a valuable treatment effect. The microorganism need a long term domestication to accommodate the quality of aniline wastewater, and the anti-shock loading capability of them is not very good as well. The culture of special group bacteria for treating aniline is a main research direction in recently years. After mutagenesis, sifting, culture, the special group bacteria can accommodate the aniline wastewater, and it also can get a better treatment effect, but the operating cost is a little bit higher. The sifting and culture of special group bacteria also need a lot of money and time.
Because the preferable tolerance of high concentration organic wastewater and the strong anti-shock loading capability of Photosynthetic Bacteria (PSB), the using of PSB in wastewater treatment field is more and more. This paper studied the feasibility of PSB combined activated sludge process to treat aniline wastewater, and studied its optimal process. Restraining the mass propagation of alga by controlling the temperature.
Studies showed that:
After being treated by PSB, the COD of aniline wastewater could be reduced from 230 4mg/L to 144-166mg/L, and the removing rate of COD was 92.8%.The content of aniline could be reduced from 28.4mg/L to 2.85mg/L. The removing rate of aniline was 90%. After being treated by activated sludge, the COD of effluent which was treated by PSB could be reduced to 84-100mg/L, and the content of aniline could be reduced to 0.71mg/L. The total COD removing rate of the whole process was 96.4%, and the total aniline removing rate of it was 97.5%. The water quality of the treated water could exceed the Nation's First grade level.
PSB and the microorganism in domestic sewage could form the multiple microorganisms. It not only could increase the biodegradability, but also could provide abundant nutrition to the activated sludge microorganism, while the PSB could keep its dominance at the same time. Some characteristics of PSB itself made it couldn't keep dominance after multiplying some time, and then the treatment effect would be affected. For resolving that question, adding fresh PSB periodically was necessary, and the expending cultivation of PSB must be executed no more 3 times.
In PSB reactor, the time of anaerobic condition could reach 44 hours. That could lengthen the time of anaerobic acidification process disguisedly. It also could deepen the anaerobic acidification of aniline, ensuring the removing rate of both COD and aniline in the followed process.
The PSB's tolerant range of temperature was wider than the alga, so the mass propagation of alga could be restrained by controlling the temperature. That could ensure the treatment effect of aniline wastewater by PSB. When the temperature was between 27≤t<29℃and 31
光合细菌(Photosynthetic Bacteria, PSB)由于其对高浓度有机废水的较好的耐受性,耐冲击能力等优点,近年来在水处理领域的应用日渐广泛。本文研究了实验室条件下,光合细菌及活性污泥法联用处理苯胺类高浓度有机废水的可行性及最佳处理工艺,并通过温度调整,抑制实验中藻类的大量繁殖。
研究结果表明：
经过PSB工艺段处理后的苯胺废水,其COD(用重铬酸钾氧化水中需氧污染物质时所消耗的氧气量)可由2304mg/L降至144-166mg/L,去除率可达92.8%。苯胺含量可由28.4mg/L降至2.85mg/L,去除率为90%。PSB段出水经过活性污泥反应器处理后,COD可降至84-100mg/L,苯胺浓度可降至0.71mg/L,整个工艺总的COD和苯胺去除率分别为96.4%和97.5%,达到国家一级排放标准。
光合细菌与生活污水中微生物可形成复合菌群,不仅增加了该废水的可生化性,也为活性污泥微生物的产生提供了丰富的营养条件,同时光合细菌还可保持优势种群。光合细菌自身的一些特点决定了它在进行一段时间的生长繁殖之后,其优势性会逐渐衰退,进而影响对废水的处理效果,为了解决这个问题,必须定期对反应器内投加新鲜菌液,且光合细菌菌液的扩大培养不能超过3次。
在PSB反应器中,每周期的厌氧时间可达44小时,变相延长了厌氧酸化状态时间,深化了苯胺类物质的酸化水解,保证了后续工艺中光合细菌及活性污泥微生物对苯胺及COD的去除率。
针对PSB反应器中出现的藻类异常繁殖现象,由于光合细菌对温度的耐受范围比藻类大,故用温度控制藻类生长。保证光合细菌处理苯胺类废水的处理效果。当反应器内温度控制在27
Because the preferable tolerance of high concentration organic wastewater and the strong anti-shock loading capability of Photosynthetic Bacteria (PSB), the using of PSB in wastewater treatment field is more and more. This paper studied the feasibility of PSB combined activated sludge process to treat aniline wastewater, and studied its optimal process. Restraining the mass propagation of alga by controlling the temperature.
Studies showed that:
After being treated by PSB, the COD of aniline wastewater could be reduced from 230 4mg/L to 144-166mg/L, and the removing rate of COD was 92.8%.The content of aniline could be reduced from 28.4mg/L to 2.85mg/L. The removing rate of aniline was 90%. After being treated by activated sludge, the COD of effluent which was treated by PSB could be reduced to 84-100mg/L, and the content of aniline could be reduced to 0.71mg/L. The total COD removing rate of the whole process was 96.4%, and the total aniline removing rate of it was 97.5%. The water quality of the treated water could exceed the Nation's First grade level.
PSB and the microorganism in domestic sewage could form the multiple microorganisms. It not only could increase the biodegradability, but also could provide abundant nutrition to the activated sludge microorganism, while the PSB could keep its dominance at the same time. Some characteristics of PSB itself made it couldn't keep dominance after multiplying some time, and then the treatment effect would be affected. For resolving that question, adding fresh PSB periodically was necessary, and the expending cultivation of PSB must be executed no more 3 times.
In PSB reactor, the time of anaerobic condition could reach 44 hours. That could lengthen the time of anaerobic acidification process disguisedly. It also could deepen the anaerobic acidification of aniline, ensuring the removing rate of both COD and aniline in the followed process.
The PSB's tolerant range of temperature was wider than the alga, so the mass propagation of alga could be restrained by controlling the temperature. That could ensure the treatment effect of aniline wastewater by PSB. When the temperature was between 27≤t<29℃and 31
引文
[1]李晓平.国内苯胺的生产消费情况及发展建议[J].氯碱工业，2006,2(2)：29-30.
[2]何杰,刘玉林,等.天然沸石用于去除水体中有机物污染物的效果[J].水处理技术,1998，24(5)：286-288.
[3]王格,卢燕,王蔺.含苯胺类染料废水处理研究[J]环境保护科学,2007,33(6)：58-60.
[4]王守恒,王爱群.醇溶苯胺黑生产废水中苯胺的回收[J]化学工业与工程技术,2009,30(3)：48-50.
[5]宁青,桑爽,张秀平.抗心律失常药盐酸索他落尔的合成[J]中国医药工业杂志,1998,29(10).
[6]蔡彦蓉.国内医药原料药苯胺市场简单分析(图).www.hc360.com.2005-08-03.
[7]佚名.苯胺的毒理特点.www.med66.com.2008-08-16.
[8]陈伟,范瑾初.超声降解水体中有机物污染物的效果及影响因素[J].给水排水,2000,26(5)：19-21.
[9]王晶,岳海燕,蔺岩.树脂吸附法处理含苯胺废水的效果研究[J].天津化工,2004,18(4)：56-57.
[10]任俊革.TiO2光催化转化NOx的研究进展[J].天津理工学院学报,2003，19(4)：13-17.
[11]郭建平.纳米氧化机理及研究进展[J].天津化工,2003,17(4)：1-3.
[12]彭书传.负载型TiO2光催化氧化法处理硝基苯酚工业废水[J].工业水处理,2003,23(11)：34-36.
[13]朱永法.不锈钢金属网上TiO2纳米薄膜光催化剂的研究[J].高等学校化学学报,2003,24(3)：465-468.
[14]范崇政.纳米TiO2光催化氧化正丙醇和异丙醇反应的研究[J].中国科学技术大学学报,2003,33(1)：99-106.
[15]钟俊波.Ti02光催化降解吡啶的研究[J].工业用水与废水,2003,34(5)：22-24.
[16]俞丹青.固载型Ti02光催化处理氨氮污水的研究[J].应用化工,2003,32(6)：17-19.
[17]徐悦华.光催化降解甲胺磷影响因素的研究[J].华南理工大学学报,2001,29(5)：68-71.
[18]王玉军,陈飞,王岩,等.中空纤维膜萃取苯酚的传质及流动特性[J].高等化学工程学报,2002,16(5)：484-489.
[19]王志强,贺高红,李宁，等.硅橡胶复合膜处理含酚废水[J].化工进 展,2006,25(3)：305-309.[20] 李磊,肖泽仪,张志炳,等.硅橡胶复合膜用于渗透蒸发的膜传质动力学(Ⅰ)：膜面上的对流传质[J].化工学报,2002,53(11)：1169-1174.
[21]HAN Shejiao, FERREIRA F C, LIVINGSTON A G. Membrane aromatic recovery system(MARS)——a new membrane process for the recovery of phenols from wastewaters[J].Journal Membrane Science,2001,188:219-233.
[22]FERREIRA F C,HAN Shejiao, LIVINGSTON A G. Recovery of aniline from aqueous solution using the membrane aromatic recovery system(MARS)[J]. Industrial & Engineering Chemical Research,2002,41:2766-2774.
[23]. FERREIRA F C,HAN Shejiao, LIVINGSTON A G, et al. Membrane aromatic recovery system (MARS):lab bench to industrial pilot scale[J].Desalination, 2002,148:267-273.
[24]张婷婷,张爱丽,周集体.活性炭吸附分离-生物再生法处理高盐苯胺废水[J]化工环保,2006，26(2)：107-110.
[25]PAN Zhiyan, YU Shangqing, HUANG Haifeng, et al. Treatment of high concentration pesticide wastewater by pressurized biochemical process[C]. Japan:The 10th Asian-Pacific Confederation of Chemical Engineering Congress,2004:1-8.
[26]陈立波,柏承志,石林锋.味精废水加压生物氧化处理工艺设计[J].给水排水,2001,27(11)：52-53.
[27]东秀珠,蔡妙英.常见细菌系统鉴定手册(第一版)[M].北京：科学出版社,2001,9-43.
[28]吴向华,杨启银,刘五星.光合细菌的研究进展及其应用[J].中国农业科技导报,2004,6(2)：35-38.
[29]Claus D, Berkeley, RCW:in Bergey's Manual of Systematic Bacteriology[J]. Williams and Wilkins Co., Baltimore. London Los Angelis, Sydney, 1984(2):1105-1139.
[30]Imhoffj, F Petrir, Su ling J. Reclassification of species of the spiral shaped phototrophic purple on sulfur bacteria of the α-Protein bacteria[J].International Journal of Systematic Bacteriology,1998(48):793-798.
[31]丁彦文,艾红.微生物在水产养殖中的应用[J].湛江海洋大学学报,2000,20(1)：68-73.
[32]龙思思,谢数涛,段舜山.光合细菌及其应用现状[J].生态科学,2002,21(1)：91-94.
[33]崔战利.光合细菌的基本特性及应用价值[J].黑龙江八一农垦大学学 报,1999,11(1)：20-25.
[34]Yu JA, Ye Y J, Lin Z X, et al. Effect of carotenoids in photo-synthetic bacteria on inhibiting lipid per-oxidation [J].Shanghai Traffic Univ.,1998,32(3):107-109.
[35]吕红,周集体,王竞.光合细菌降解有机污染物的研究进展[J].工业水处理,2003,23(10)：9-12.
[36]宋志文,郭本华,曹军.光合细菌及其在化工有机废水处理方面的应用[J]化工环保,2004,23(4)：209-212.
[37]陈福杰.光合细菌的性质和应用[J].生物学教学,2000,25(1)：46-47.
[38]谢娟,艾翠玲.光合细菌对味精废水处理效果的影响研究[J].工业安全与环保,2006,32(4)：16-18.
[39]温志英,安郁琴.光合细菌在我国食品工业废水处理中的应用[J].陕西科技大学学报,2006,24(3)：146-149.
[40]王菲凤,刘文伟.应用光合细菌处理味精废水[J].福建环境,1999,16(2)：17-20.
[41]孟春,邱荔,石贤爱,等.厌氧-微氧生物法处理味精生产废水的研究[J].福州大学学报,2000,28(1)：106-109.
[42]程树培,崔益斌,申为民,等.外循环气升式反应器中光合细菌处理味精废水的研究[J].环境科学,1994,15(2)：6-10.
[43]刘军义,李丽华.光合细菌与酵母菌综合处理柠檬酸废水的初步研究[J].微生物学杂志,1998,18(3)：30-33.
[44]朱核光.光合细菌法处理酒糟废水的试验研究[J].上海环境科学,1995,14(1)：8-10.
[45]曾宇,成华.光合细菌法处理酒糟废水[J].四川大学学报,2002,39(2)：382-384.
[46]王宇新,刘春朝,钱新民.光合细菌处理淀粉废水的中试研究[J].环境科学,1994,16(3)：39-40.
[47]闫琦涛,王维坚,韩克文等.玉米淀粉黄浆水PSB资源化开发的应用前景[J].吉林粮食高等专科学校学报,2000,15(4)：18-20.
[48]郑爱榕,蔡阿根,许伟斌等.光合细菌和螺旋藻对啤酒废水的净化与利用[J].环境科学学报,1999,19(1)：22-27.
[49]谢红刚,王三反,褚润.光合细菌对啤酒废水处理的研究[J].工业用水与废水,2006,37(5)：38-40.
[50]刘双江，杨惠芳,周培瑾等.固定化光合细菌处理豆制品废水产氢研究[J].环境科学,1995,16(1)：42-44.
[51]何萍,吴海青,杨启银等.光合细菌处理豆制品废水的研究[J].江苏农业研究,2001,22(4)：63-65.
[52]何萍,杨启银,陈育如等.光合细菌与小球藻复合处理豆制品废水[J].无锡轻工大学学报,2001,20(6)：578-581.
[53]王有志,王凤君,鲍利等.光合细菌处理中药废水的试验研究[J].东北农业大学学报,2005,36(5)：579-583.
[54]陈繁忠.利用光合细菌处理养猪场污水的试验研究[J].环境工程,2000,18(5)：10-12.
[55]谢冰.有机废水的复合光合细菌法处理及机理初探[J].应用与环境生物学报,1999,(5)：88-90.
[56]黄宝兴,李兰生,赵亮等.固定化海洋光合细菌处理生活污水的研究[J].海洋湖沼通报,2006,(2)：69-74.
[57]凌云,冯贵颖,刘建党,等.酵母菌——光合细菌联用处理皂素废水的试验研究[J].西北农业学报,2006,15(1)：109-112.
[58]应翼,张信娣,张雅萍.光合细菌的分离鉴定及对印染污水的处理[J].绍兴文理学院学报,2006,26(7)：40-43.
[59]冯国丽.光合细菌法处理焦化废水[J].科技情报开发与经济,2005,15(16)：132-133.
[60]谷军,杨旭,田洁萍.光合细菌在水产养殖业的应用研究概况[J].黑龙江水产,2002,(4)：1-4.
[61]张道南,孙其焕,陈乃松.红螺菌科光合细菌的分离培养及其作为鱼虾类饵料添加剂的初步研究[J].水产学报,1988,12(4)：367-369.
[62]杨素萍,赵春贵,曲音波.光合细菌产氢研究进展[J].水生生物学报,2003,27(1)：85-91.
[63]Sweet W J, Burris R H. Inhibition of nitrogenase activity by NH+4 in R hodospirillum rubrum [J].J.Bacteriol,1981,145(2):824-831.
[64]翁酥颖.环境微生物学[M].北京：科学出版社,1985,21-45.
[65]丁雷,赵德炳.光合细菌在水产养殖上的应用研究与进展[J].水利渔业,2001,21(1)：23-25.
[66]王永忠,廖强,朱恂.底物初始浓度对光合细菌产氢动力学特性的影响[J].环境工程学报,2007,(7)：127-131.
[67]周汝雁,张全国.环流罐式光合生物反应器连续产氢研究[J].武汉理工大学学报,2006,(21)：132-138.
[68]康铸慧,王磊,郑广宏.微生物产氢研究[J].工业微生物,2005,35(2)：41-49.
[69]Ooshima H, Takakuwa S. Production of hydrogen by a hydrogenasedeficient mutant of R hodobacter capsulatus [J]. J. Ferment Bioeng,1998,85(5):470-475.
[70]Kern M, Klipp W, Klemme J H. Increased nitrogenase-dependent H2 production by hup mutants of R hodospirillum rubrum [J]. Appl Env. Microbiol,1994, 60(6):1786-1774.
[71]Nakada E, Nishikate S. Photosynthetic bacterial hydrogen production combined with a fuel cell[J].Int. J. Hydrogen Energy,1999,(24):1053-1057.
[72]Otsuki T, Uchiyama S, John R, et al. Hydrogen production by floating type bioreactor [M].New York:Plenum Publishing,1998,369-374.
[73]Ogbonna J C, Soejima T, Tanaka H. An integrated solar and artificial light system for internal illumination of photo bioreactors[J]. Biothchnol,1999,(70): 289-297.
[74]Miyake J, Tatsuki W. Simulation of the daily sunlight illumination pattern for bacterial photo-hydrigen production [J].J.BiosciBioen,1999,88(6):659-663.
[75]Koku H, Eroglu I. Kinetics of biological hydrogen production Rhodobacter sphaetoides OU 001 [J]. Int. J. Hydrogen Energy,2003,(28):381-388.
[76]Turkarslan S, Yigit D O. Photo biological hydrogen production Rhodobacter sphaetoides OU 001 by utilization of waste water from milk industry[M]. New York:Plenum Press,1998,274-385.
[77]Zhu H, Suzuki T. Hydrogen production from tofu wastewater R hodobacter phaetoies immobilized in agargels[J]. Int. J. Hydrgen Energy,1999,(24): 305-310.
[78]Yu JA, Zhang C K, Chen, et al. Study on the mechanism eria on the anti-oxidation[J].Chin.J.Lumines,2000,21(3):219-223.
[79]Gao L, Zhang Z M, Yang G E. Immunity function and the antitum or effect of photosynthetic bacteria preparation sontumorigenic mice[J]. J. ShanxiMed. Univ.,2004,35(1):8-9.
[80]Yu J A, Zhang C K, Fan X B, et al. Evaluation on the immunology activity of R hodobactersphaeroides No.1 strain [J].China J.Microecol,2002,14(2):14-16.
[81]韩梅,陈锡时,张良.光合细菌研究概况及其应用进展[J].沈阳农业大学学报,2002,33(5)：387-389.
[82]刘春朝,钱新民.光合细菌发酵产类胡萝素的中试研究[J].食品与发酵工业,1994,(6)：47-49.
[83]李福枝,刘飞,江南.沼泽红假单胞菌产类胡萝卜素培养条件的优化研究[J].食品与机械,2007,(1)：54-59.
[84]孙军德,张恩禄,赵春燕.沼泽红假单胞菌培养条件研究[J].沈阳农业大学学报,2003,(1)：29-32.
[85]钱森和,厉荣玉,汤斌.紫色非硫光合细菌培养基条件优化的研究[J].安徽工程 科技学院学报(自然科学版),2007,(2)：7-11.
[86]顾青,梁新乐,励建荣.光合细菌R1发酵产类胡萝卜素的研究[J].食品与发酵工业,2001,(10)：20-24.
[87]Hajime Yoshida, Yukinobu Kotani, KeikoOchiai, et al. Production of ubiquino-ne 10 using bacteria [J]. The Journal of General and Applied Micro-biology, 1998,(44):19-26.
[88]袁静,魏泓.光合细菌产辅酶Q10发酵条件的研究[J].氨基酸和生物资源,2003,(2)：37-40.
[89]王春林,韩进.辅酶Q10口服剂型及其临床应用[J].世界临床药物,1986,(2)：33-36.
[90]方立超,黄雪峰,杜珍辉.产生辅酶Q10的光合细菌菌株的分离及鉴定[J].微生物学报,2005,(5)：32-35.
[91]赵玲,沈朝平,陈为民.光合细菌(PSB)在稚鳖养殖中的应用[J].内陆水产,2005,(10)：17.
[92]李东风.光合细菌的开发应用动态[J].微生物学杂志,1998，18(2)：44-50.
[93]周茂洪,何洋.光合细菌及其在养殖业中应用研究进展[J].温州大学学报,2001,(1)：54-57.
[94]俞吉安,林克新,言世贤.应用光合细菌铒料添加剂养鱼的研究报告[J].淡水渔业,1991,(3)：10-13.
[95]张信娣,陈瑛.光合细菌对三角帆蚌养殖水体水质的影响[J].淡水渔业,2007,(1)：30-34.
[96]王俊卿,张肇铭.光合细菌对鸡蛋产量及抗氧化活性的影响[J].中国家禽,2002,(11)：35-38.
[97]R.E布坎南,N.E吉本斯,等.伯杰细菌鉴定手册第八版[M].科学出版社,1984,北京.
[98]J G Ormerod. The Phototrophic Bacteria:Anaerobic Life in the light[M]. USA: University of California Press,1983.
[99]史家棵,徐亚同,张圣章.环境微生物学[M].上海：华东师范大学出版社,1993.
[100]朱章玉,俞吉安,林志新,李宝.光合细菌的研究及其利用[M].上海：上海交通大学出版社,1991.
[101]刘如林,刁虎欣,梁凤来,赵大健.光合细菌及其应用[M].北京：中国农业科技出版社,1991.
[102]沈同,王镜岩.生物化学(第二版)[M].北京：高等教育出版社,1990.
[103]Harwood, S., et al. Shedding light on anaerobic benzene degradation:a process unique to prokaryotes [J]. J. Bacterial,1997,179:2,301-309.
[104]H.Sawada,P.L.Rogers.Photosynthetic Bacteria in Waste Treatment——Pure Culture Studies with R hodopseudomonas capsulata.J.Ferment Technol, 1977,55(4):297-310.
[105]孔秀琴,郑伟华.光合细菌(PSB)新膜法工艺处理高浓度淀粉废水实验研究[J].2008年高浊度水净化及污水资源化工程学术暨建设成果论文集,2008.09,151-157.
[106]黄翔峰,李春鞠,章非娟.光合细菌法处理高浓度有机废水工艺探讨[J].中国给水排水,2005,21(2)：27-30.
[107]谢冰,史家梁,徐亚同.有机废水的复合光合细菌法处理及机理初探[J]应用与环境生物学报,1999,Suppl(5):88-90.
[108]王小文,张雁秋.水污染控制工程[M].煤炭工业出版社,2002：271-273.
[109]陈吉升,苗群,刘志强,等.难降解氯碱污水的预处理试验研究[J].工业安全和环保.2005,34(1)：12-15.
[110]王德强.生活污水中藻类生长对培养光合细菌的影响[J].安徽工业大学学报,2002,19(2)：120-122.
[111]佚名.衣藻属[EB/OL].(2007-01-06)[2007-01-06]. http://baike.baidu.com/view/699544.htm.
[112]中国科学院南京地质古生物研究所.柄裸藻属[EB/OL].(2007-06-15) [2007-06-18].http://www.uua.cn/Paleobotany/show.6052-1.html.
[113]中国科学院南京地质古生物研究所.双鞭藻属[EB/OL].(2007-06-15) [2007-06-18].http://www.uua.cn/Paleobotany/show-6052-1.html.
[114]GAO M.The responses of antioxidases of the different seaweeds to the different levels of temperatures and pH[J].Journal of Ocean University of qingdao,1953, 28(3):21-27.
[115]丁燏,缪锦来,王全富.等.温度对南极衣藻ICE-L(Chlamy-domonassp.ICE-L)谷胱甘肽含量及其相关酶活性的影响[J].海洋与湖沼,2006,37(2)：154-161.
[116]孙菊燕,黄鹤忠.环境因子对海洋藻类酶活性的影响及其应用[J].海洋科学进展,2005，,23(12)：74-79.
[117]M.Kobayashi.Advances in Agricultural Microbiology(Edited by Rao N S S)[M]. London:Butterworth Scientific.,1982:643-661.
[2]何杰,刘玉林,等.天然沸石用于去除水体中有机物污染物的效果[J].水处理技术,1998，24(5)：286-288.
[3]王格,卢燕,王蔺.含苯胺类染料废水处理研究[J]环境保护科学,2007,33(6)：58-60.
[4]王守恒,王爱群.醇溶苯胺黑生产废水中苯胺的回收[J]化学工业与工程技术,2009,30(3)：48-50.
[5]宁青,桑爽,张秀平.抗心律失常药盐酸索他落尔的合成[J]中国医药工业杂志,1998,29(10).
[6]蔡彦蓉.国内医药原料药苯胺市场简单分析(图).www.hc360.com.2005-08-03.
[7]佚名.苯胺的毒理特点.www.med66.com.2008-08-16.
[8]陈伟,范瑾初.超声降解水体中有机物污染物的效果及影响因素[J].给水排水,2000,26(5)：19-21.
[9]王晶,岳海燕,蔺岩.树脂吸附法处理含苯胺废水的效果研究[J].天津化工,2004,18(4)：56-57.
[10]任俊革.TiO2光催化转化NOx的研究进展[J].天津理工学院学报,2003，19(4)：13-17.
[11]郭建平.纳米氧化机理及研究进展[J].天津化工,2003,17(4)：1-3.
[12]彭书传.负载型TiO2光催化氧化法处理硝基苯酚工业废水[J].工业水处理,2003,23(11)：34-36.
[13]朱永法.不锈钢金属网上TiO2纳米薄膜光催化剂的研究[J].高等学校化学学报,2003,24(3)：465-468.
[14]范崇政.纳米TiO2光催化氧化正丙醇和异丙醇反应的研究[J].中国科学技术大学学报,2003,33(1)：99-106.
[15]钟俊波.Ti02光催化降解吡啶的研究[J].工业用水与废水,2003,34(5)：22-24.
[16]俞丹青.固载型Ti02光催化处理氨氮污水的研究[J].应用化工,2003,32(6)：17-19.
[17]徐悦华.光催化降解甲胺磷影响因素的研究[J].华南理工大学学报,2001,29(5)：68-71.
[18]王玉军,陈飞,王岩,等.中空纤维膜萃取苯酚的传质及流动特性[J].高等化学工程学报,2002,16(5)：484-489.
[19]王志强,贺高红,李宁，等.硅橡胶复合膜处理含酚废水[J].化工进 展,2006,25(3)：305-309.[20] 李磊,肖泽仪,张志炳,等.硅橡胶复合膜用于渗透蒸发的膜传质动力学(Ⅰ)：膜面上的对流传质[J].化工学报,2002,53(11)：1169-1174.
[21]HAN Shejiao, FERREIRA F C, LIVINGSTON A G. Membrane aromatic recovery system(MARS)——a new membrane process for the recovery of phenols from wastewaters[J].Journal Membrane Science,2001,188:219-233.
[22]FERREIRA F C,HAN Shejiao, LIVINGSTON A G. Recovery of aniline from aqueous solution using the membrane aromatic recovery system(MARS)[J]. Industrial & Engineering Chemical Research,2002,41:2766-2774.
[23]. FERREIRA F C,HAN Shejiao, LIVINGSTON A G, et al. Membrane aromatic recovery system (MARS):lab bench to industrial pilot scale[J].Desalination, 2002,148:267-273.
[24]张婷婷,张爱丽,周集体.活性炭吸附分离-生物再生法处理高盐苯胺废水[J]化工环保,2006，26(2)：107-110.
[25]PAN Zhiyan, YU Shangqing, HUANG Haifeng, et al. Treatment of high concentration pesticide wastewater by pressurized biochemical process[C]. Japan:The 10th Asian-Pacific Confederation of Chemical Engineering Congress,2004:1-8.
[26]陈立波,柏承志,石林锋.味精废水加压生物氧化处理工艺设计[J].给水排水,2001,27(11)：52-53.
[27]东秀珠,蔡妙英.常见细菌系统鉴定手册(第一版)[M].北京：科学出版社,2001,9-43.
[28]吴向华,杨启银,刘五星.光合细菌的研究进展及其应用[J].中国农业科技导报,2004,6(2)：35-38.
[29]Claus D, Berkeley, RCW:in Bergey's Manual of Systematic Bacteriology[J]. Williams and Wilkins Co., Baltimore. London Los Angelis, Sydney, 1984(2):1105-1139.
[30]Imhoffj, F Petrir, Su ling J. Reclassification of species of the spiral shaped phototrophic purple on sulfur bacteria of the α-Protein bacteria[J].International Journal of Systematic Bacteriology,1998(48):793-798.
[31]丁彦文,艾红.微生物在水产养殖中的应用[J].湛江海洋大学学报,2000,20(1)：68-73.
[32]龙思思,谢数涛,段舜山.光合细菌及其应用现状[J].生态科学,2002,21(1)：91-94.
[33]崔战利.光合细菌的基本特性及应用价值[J].黑龙江八一农垦大学学 报,1999,11(1)：20-25.
[34]Yu JA, Ye Y J, Lin Z X, et al. Effect of carotenoids in photo-synthetic bacteria on inhibiting lipid per-oxidation [J].Shanghai Traffic Univ.,1998,32(3):107-109.
[35]吕红,周集体,王竞.光合细菌降解有机污染物的研究进展[J].工业水处理,2003,23(10)：9-12.
[36]宋志文,郭本华,曹军.光合细菌及其在化工有机废水处理方面的应用[J]化工环保,2004,23(4)：209-212.
[37]陈福杰.光合细菌的性质和应用[J].生物学教学,2000,25(1)：46-47.
[38]谢娟,艾翠玲.光合细菌对味精废水处理效果的影响研究[J].工业安全与环保,2006,32(4)：16-18.
[39]温志英,安郁琴.光合细菌在我国食品工业废水处理中的应用[J].陕西科技大学学报,2006,24(3)：146-149.
[40]王菲凤,刘文伟.应用光合细菌处理味精废水[J].福建环境,1999,16(2)：17-20.
[41]孟春,邱荔,石贤爱,等.厌氧-微氧生物法处理味精生产废水的研究[J].福州大学学报,2000,28(1)：106-109.
[42]程树培,崔益斌,申为民,等.外循环气升式反应器中光合细菌处理味精废水的研究[J].环境科学,1994,15(2)：6-10.
[43]刘军义,李丽华.光合细菌与酵母菌综合处理柠檬酸废水的初步研究[J].微生物学杂志,1998,18(3)：30-33.
[44]朱核光.光合细菌法处理酒糟废水的试验研究[J].上海环境科学,1995,14(1)：8-10.
[45]曾宇,成华.光合细菌法处理酒糟废水[J].四川大学学报,2002,39(2)：382-384.
[46]王宇新,刘春朝,钱新民.光合细菌处理淀粉废水的中试研究[J].环境科学,1994,16(3)：39-40.
[47]闫琦涛,王维坚,韩克文等.玉米淀粉黄浆水PSB资源化开发的应用前景[J].吉林粮食高等专科学校学报,2000,15(4)：18-20.
[48]郑爱榕,蔡阿根,许伟斌等.光合细菌和螺旋藻对啤酒废水的净化与利用[J].环境科学学报,1999,19(1)：22-27.
[49]谢红刚,王三反,褚润.光合细菌对啤酒废水处理的研究[J].工业用水与废水,2006,37(5)：38-40.
[50]刘双江，杨惠芳,周培瑾等.固定化光合细菌处理豆制品废水产氢研究[J].环境科学,1995,16(1)：42-44.
[51]何萍,吴海青,杨启银等.光合细菌处理豆制品废水的研究[J].江苏农业研究,2001,22(4)：63-65.
[52]何萍,杨启银,陈育如等.光合细菌与小球藻复合处理豆制品废水[J].无锡轻工大学学报,2001,20(6)：578-581.
[53]王有志,王凤君,鲍利等.光合细菌处理中药废水的试验研究[J].东北农业大学学报,2005,36(5)：579-583.
[54]陈繁忠.利用光合细菌处理养猪场污水的试验研究[J].环境工程,2000,18(5)：10-12.
[55]谢冰.有机废水的复合光合细菌法处理及机理初探[J].应用与环境生物学报,1999,(5)：88-90.
[56]黄宝兴,李兰生,赵亮等.固定化海洋光合细菌处理生活污水的研究[J].海洋湖沼通报,2006,(2)：69-74.
[57]凌云,冯贵颖,刘建党,等.酵母菌——光合细菌联用处理皂素废水的试验研究[J].西北农业学报,2006,15(1)：109-112.
[58]应翼,张信娣,张雅萍.光合细菌的分离鉴定及对印染污水的处理[J].绍兴文理学院学报,2006,26(7)：40-43.
[59]冯国丽.光合细菌法处理焦化废水[J].科技情报开发与经济,2005,15(16)：132-133.
[60]谷军,杨旭,田洁萍.光合细菌在水产养殖业的应用研究概况[J].黑龙江水产,2002,(4)：1-4.
[61]张道南,孙其焕,陈乃松.红螺菌科光合细菌的分离培养及其作为鱼虾类饵料添加剂的初步研究[J].水产学报,1988,12(4)：367-369.
[62]杨素萍,赵春贵,曲音波.光合细菌产氢研究进展[J].水生生物学报,2003,27(1)：85-91.
[63]Sweet W J, Burris R H. Inhibition of nitrogenase activity by NH+4 in R hodospirillum rubrum [J].J.Bacteriol,1981,145(2):824-831.
[64]翁酥颖.环境微生物学[M].北京：科学出版社,1985,21-45.
[65]丁雷,赵德炳.光合细菌在水产养殖上的应用研究与进展[J].水利渔业,2001,21(1)：23-25.
[66]王永忠,廖强,朱恂.底物初始浓度对光合细菌产氢动力学特性的影响[J].环境工程学报,2007,(7)：127-131.
[67]周汝雁,张全国.环流罐式光合生物反应器连续产氢研究[J].武汉理工大学学报,2006,(21)：132-138.
[68]康铸慧,王磊,郑广宏.微生物产氢研究[J].工业微生物,2005,35(2)：41-49.
[69]Ooshima H, Takakuwa S. Production of hydrogen by a hydrogenasedeficient mutant of R hodobacter capsulatus [J]. J. Ferment Bioeng,1998,85(5):470-475.
[70]Kern M, Klipp W, Klemme J H. Increased nitrogenase-dependent H2 production by hup mutants of R hodospirillum rubrum [J]. Appl Env. Microbiol,1994, 60(6):1786-1774.
[71]Nakada E, Nishikate S. Photosynthetic bacterial hydrogen production combined with a fuel cell[J].Int. J. Hydrogen Energy,1999,(24):1053-1057.
[72]Otsuki T, Uchiyama S, John R, et al. Hydrogen production by floating type bioreactor [M].New York:Plenum Publishing,1998,369-374.
[73]Ogbonna J C, Soejima T, Tanaka H. An integrated solar and artificial light system for internal illumination of photo bioreactors[J]. Biothchnol,1999,(70): 289-297.
[74]Miyake J, Tatsuki W. Simulation of the daily sunlight illumination pattern for bacterial photo-hydrigen production [J].J.BiosciBioen,1999,88(6):659-663.
[75]Koku H, Eroglu I. Kinetics of biological hydrogen production Rhodobacter sphaetoides OU 001 [J]. Int. J. Hydrogen Energy,2003,(28):381-388.
[76]Turkarslan S, Yigit D O. Photo biological hydrogen production Rhodobacter sphaetoides OU 001 by utilization of waste water from milk industry[M]. New York:Plenum Press,1998,274-385.
[77]Zhu H, Suzuki T. Hydrogen production from tofu wastewater R hodobacter phaetoies immobilized in agargels[J]. Int. J. Hydrgen Energy,1999,(24): 305-310.
[78]Yu JA, Zhang C K, Chen, et al. Study on the mechanism eria on the anti-oxidation[J].Chin.J.Lumines,2000,21(3):219-223.
[79]Gao L, Zhang Z M, Yang G E. Immunity function and the antitum or effect of photosynthetic bacteria preparation sontumorigenic mice[J]. J. ShanxiMed. Univ.,2004,35(1):8-9.
[80]Yu J A, Zhang C K, Fan X B, et al. Evaluation on the immunology activity of R hodobactersphaeroides No.1 strain [J].China J.Microecol,2002,14(2):14-16.
[81]韩梅,陈锡时,张良.光合细菌研究概况及其应用进展[J].沈阳农业大学学报,2002,33(5)：387-389.
[82]刘春朝,钱新民.光合细菌发酵产类胡萝素的中试研究[J].食品与发酵工业,1994,(6)：47-49.
[83]李福枝,刘飞,江南.沼泽红假单胞菌产类胡萝卜素培养条件的优化研究[J].食品与机械,2007,(1)：54-59.
[84]孙军德,张恩禄,赵春燕.沼泽红假单胞菌培养条件研究[J].沈阳农业大学学报,2003,(1)：29-32.
[85]钱森和,厉荣玉,汤斌.紫色非硫光合细菌培养基条件优化的研究[J].安徽工程 科技学院学报(自然科学版),2007,(2)：7-11.
[86]顾青,梁新乐,励建荣.光合细菌R1发酵产类胡萝卜素的研究[J].食品与发酵工业,2001,(10)：20-24.
[87]Hajime Yoshida, Yukinobu Kotani, KeikoOchiai, et al. Production of ubiquino-ne 10 using bacteria [J]. The Journal of General and Applied Micro-biology, 1998,(44):19-26.
[88]袁静,魏泓.光合细菌产辅酶Q10发酵条件的研究[J].氨基酸和生物资源,2003,(2)：37-40.
[89]王春林,韩进.辅酶Q10口服剂型及其临床应用[J].世界临床药物,1986,(2)：33-36.
[90]方立超,黄雪峰,杜珍辉.产生辅酶Q10的光合细菌菌株的分离及鉴定[J].微生物学报,2005,(5)：32-35.
[91]赵玲,沈朝平,陈为民.光合细菌(PSB)在稚鳖养殖中的应用[J].内陆水产,2005,(10)：17.
[92]李东风.光合细菌的开发应用动态[J].微生物学杂志,1998，18(2)：44-50.
[93]周茂洪,何洋.光合细菌及其在养殖业中应用研究进展[J].温州大学学报,2001,(1)：54-57.
[94]俞吉安,林克新,言世贤.应用光合细菌铒料添加剂养鱼的研究报告[J].淡水渔业,1991,(3)：10-13.
[95]张信娣,陈瑛.光合细菌对三角帆蚌养殖水体水质的影响[J].淡水渔业,2007,(1)：30-34.
[96]王俊卿,张肇铭.光合细菌对鸡蛋产量及抗氧化活性的影响[J].中国家禽,2002,(11)：35-38.
[97]R.E布坎南,N.E吉本斯,等.伯杰细菌鉴定手册第八版[M].科学出版社,1984,北京.
[98]J G Ormerod. The Phototrophic Bacteria:Anaerobic Life in the light[M]. USA: University of California Press,1983.
[99]史家棵,徐亚同,张圣章.环境微生物学[M].上海：华东师范大学出版社,1993.
[100]朱章玉,俞吉安,林志新,李宝.光合细菌的研究及其利用[M].上海：上海交通大学出版社,1991.
[101]刘如林,刁虎欣,梁凤来,赵大健.光合细菌及其应用[M].北京：中国农业科技出版社,1991.
[102]沈同,王镜岩.生物化学(第二版)[M].北京：高等教育出版社,1990.
[103]Harwood, S., et al. Shedding light on anaerobic benzene degradation:a process unique to prokaryotes [J]. J. Bacterial,1997,179:2,301-309.
[104]H.Sawada,P.L.Rogers.Photosynthetic Bacteria in Waste Treatment——Pure Culture Studies with R hodopseudomonas capsulata.J.Ferment Technol, 1977,55(4):297-310.
[105]孔秀琴,郑伟华.光合细菌(PSB)新膜法工艺处理高浓度淀粉废水实验研究[J].2008年高浊度水净化及污水资源化工程学术暨建设成果论文集,2008.09,151-157.
[106]黄翔峰,李春鞠,章非娟.光合细菌法处理高浓度有机废水工艺探讨[J].中国给水排水,2005,21(2)：27-30.
[107]谢冰,史家梁,徐亚同.有机废水的复合光合细菌法处理及机理初探[J]应用与环境生物学报,1999,Suppl(5):88-90.
[108]王小文,张雁秋.水污染控制工程[M].煤炭工业出版社,2002：271-273.
[109]陈吉升,苗群,刘志强,等.难降解氯碱污水的预处理试验研究[J].工业安全和环保.2005,34(1)：12-15.
[110]王德强.生活污水中藻类生长对培养光合细菌的影响[J].安徽工业大学学报,2002,19(2)：120-122.
[111]佚名.衣藻属[EB/OL].(2007-01-06)[2007-01-06]. http://baike.baidu.com/view/699544.htm.
[112]中国科学院南京地质古生物研究所.柄裸藻属[EB/OL].(2007-06-15) [2007-06-18].http://www.uua.cn/Paleobotany/show.6052-1.html.
[113]中国科学院南京地质古生物研究所.双鞭藻属[EB/OL].(2007-06-15) [2007-06-18].http://www.uua.cn/Paleobotany/show-6052-1.html.
[114]GAO M.The responses of antioxidases of the different seaweeds to the different levels of temperatures and pH[J].Journal of Ocean University of qingdao,1953, 28(3):21-27.
[115]丁燏,缪锦来,王全富.等.温度对南极衣藻ICE-L(Chlamy-domonassp.ICE-L)谷胱甘肽含量及其相关酶活性的影响[J].海洋与湖沼,2006,37(2)：154-161.
[116]孙菊燕,黄鹤忠.环境因子对海洋藻类酶活性的影响及其应用[J].海洋科学进展,2005，,23(12)：74-79.
[117]M.Kobayashi.Advances in Agricultural Microbiology(Edited by Rao N S S)[M]. London:Butterworth Scientific.,1982:643-661.