磁场—趋磁细菌工艺处理含Cu~（2+）、Zn~（2+）废水的应用研究
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摘要
近年来,利用磁场-趋磁细菌工艺处理重金属废水是国内外一个新型的研究领域,它具有速度快、选择性高等优点,而且不造成二次污染,以该复合工艺处理重金属废水,这方面的研究鲜有报道。
论文通过实验研究,证明该复合工艺用于处理重金属废水及趋磁细菌菌体的吸附分离是可行和有效的。
论文通过研究发现:
1)微好氧条件下较好氧条件下有利于趋磁细菌的培养。微好氧培养条件下趋磁细菌菌量较多,磁性较强。
2)分离筛选出两个磁性较强的单株菌J-1和J-3,J-1为球菌,J-3为杆菌,且两种菌都呈革兰氏阴性。J-1菌对含Cu~(2+)、Zn~(2+)废水的去除率分别达到61.2%,55.7%;J-3菌对含Cu~(2+)、Zn~(2+)废水的去除率分别达到45.2%,41.8%。J-1菌吸附效果较好。3)J-1菌吸附Cu~(2+)最佳操作条件为pH=5、温度25℃、吸附时间1.5h、趋磁细菌量为60g/l。吸附Zn~(2+)最佳操作条件为pH=5、温度25℃、吸附时间2h、趋磁细菌量为80g/l。趋磁细菌对Cu~(2+)、Zn~(2+)的吸附等温线较符合Langmuir模型。
4)Cu~(2+)、Zn~(2+)共存体系在两种离子浓度相同时,与单一体系相比,吸附率分别降低了36.76%,24.5%。在Cu~(2+)、Zn~(2+)二元竞争吸附体系中优先吸附Zn~(2+),Zn~(2+)与趋磁细菌的亲和力大Cu~(2+)。
5)利用复合工艺对含Cu~(2+)、Zn~(2+)废水进行处理。发现磁分离器对附着有重金属离子的趋磁细菌有吸附作用,溶液OD600值分别降低0.128和0.107。对铜、锌离子去除率分别为66%,62.5%去除效果理想。
Magnetic Field-Magnetotactic Bacteria(MTB) Comprehensive Technology removing heavy mental from wastewater has become a new hotspot reeently. It not only has the advantages of high speed and high seleetivity but also donotereate the second Pollution, microorganism which adsorb heavy metal ions can be easily isolated from the wastewater. But there are few reports concerning application of the technologies in dealing with the wastewater.
In the study of this thesis,the result using magnetic separators absorbed Cu~(2+)、Zn~(2+) indicated the method was effective and feasible.
The results of the research show us:
1) MTB are more easily acclimated in slightly aerobic condition.the quantity and magnetism of MTB are more greater in this condition.
2) The two strains named J-1,J-3 can remove Cu~(2+),Zn~(2+). Remove efficiency of J-1 is 61.2%,55.7%;and J-3 is 5.2%,41.8%。J-1 have higher remove efficiency.
3) The best condition absorb Cu~(2+) is pH=5,T=25℃、t=1.5h、MTB=60g/l。the best condition absorb Zn~(2+) is pH=5,T=25℃, t= 2h、MTB=80g/l。A comparison of different isotherm models revealed that Langmuir model fitted the experimental data best.
4) When Cu~(2+),Zn~(2+)coexist in water the remove efficiency descend 36.76%,24.5% respectively and MTB adsorb Zn~(2+) preferential.
5) Use magnetic separators dealing with the wastewater.the OD600 have descend and the remove efficiency of heavy metal irons have arised .
论文通过实验研究,证明该复合工艺用于处理重金属废水及趋磁细菌菌体的吸附分离是可行和有效的。
论文通过研究发现:
1)微好氧条件下较好氧条件下有利于趋磁细菌的培养。微好氧培养条件下趋磁细菌菌量较多,磁性较强。
2)分离筛选出两个磁性较强的单株菌J-1和J-3,J-1为球菌,J-3为杆菌,且两种菌都呈革兰氏阴性。J-1菌对含Cu~(2+)、Zn~(2+)废水的去除率分别达到61.2%,55.7%;J-3菌对含Cu~(2+)、Zn~(2+)废水的去除率分别达到45.2%,41.8%。J-1菌吸附效果较好。3)J-1菌吸附Cu~(2+)最佳操作条件为pH=5、温度25℃、吸附时间1.5h、趋磁细菌量为60g/l。吸附Zn~(2+)最佳操作条件为pH=5、温度25℃、吸附时间2h、趋磁细菌量为80g/l。趋磁细菌对Cu~(2+)、Zn~(2+)的吸附等温线较符合Langmuir模型。
4)Cu~(2+)、Zn~(2+)共存体系在两种离子浓度相同时,与单一体系相比,吸附率分别降低了36.76%,24.5%。在Cu~(2+)、Zn~(2+)二元竞争吸附体系中优先吸附Zn~(2+),Zn~(2+)与趋磁细菌的亲和力大Cu~(2+)。
5)利用复合工艺对含Cu~(2+)、Zn~(2+)废水进行处理。发现磁分离器对附着有重金属离子的趋磁细菌有吸附作用,溶液OD600值分别降低0.128和0.107。对铜、锌离子去除率分别为66%,62.5%去除效果理想。
Magnetic Field-Magnetotactic Bacteria(MTB) Comprehensive Technology removing heavy mental from wastewater has become a new hotspot reeently. It not only has the advantages of high speed and high seleetivity but also donotereate the second Pollution, microorganism which adsorb heavy metal ions can be easily isolated from the wastewater. But there are few reports concerning application of the technologies in dealing with the wastewater.
In the study of this thesis,the result using magnetic separators absorbed Cu~(2+)、Zn~(2+) indicated the method was effective and feasible.
The results of the research show us:
1) MTB are more easily acclimated in slightly aerobic condition.the quantity and magnetism of MTB are more greater in this condition.
2) The two strains named J-1,J-3 can remove Cu~(2+),Zn~(2+). Remove efficiency of J-1 is 61.2%,55.7%;and J-3 is 5.2%,41.8%。J-1 have higher remove efficiency.
3) The best condition absorb Cu~(2+) is pH=5,T=25℃、t=1.5h、MTB=60g/l。the best condition absorb Zn~(2+) is pH=5,T=25℃, t= 2h、MTB=80g/l。A comparison of different isotherm models revealed that Langmuir model fitted the experimental data best.
4) When Cu~(2+),Zn~(2+)coexist in water the remove efficiency descend 36.76%,24.5% respectively and MTB adsorb Zn~(2+) preferential.
5) Use magnetic separators dealing with the wastewater.the OD600 have descend and the remove efficiency of heavy metal irons have arised .
引文
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